CN103461116B - Method for early screening of fine varieties of nobile-types - Google Patents
Method for early screening of fine varieties of nobile-types Download PDFInfo
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- CN103461116B CN103461116B CN201310397973.9A CN201310397973A CN103461116B CN 103461116 B CN103461116 B CN 103461116B CN 201310397973 A CN201310397973 A CN 201310397973A CN 103461116 B CN103461116 B CN 103461116B
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- noble dendrobium
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Abstract
The invention discloses a method for early screening of fine varieties of nobile-types. The method comprises the following steps of a, measuring plant heights and leaf lengths of annual cutting seedlings; b, calculating the quality comprehensive score of a nobile-types flower by substituting the average values of the plant heights and the leaf lengths into a regression equation y=0.511*1 (plant height)+0.845*2 (leaf length)-7.247; and c, predicating the quality of the nobile-types flower according to the quality comprehensive score of the nobile-types flower and variety grading reference criteria. The method provided by the invention improves the variety screening efficiency, shortens the screening cycle, and saves the production cost.
Description
Technical field
The present invention relates to plant variety screening technique, particularly a kind of method of spring stem of noble dendrobium kind early screening.
Background technology
The spring stem of noble dendrobium is liked by global consumer deeply because of features such as pattern are abundant, the florescence is long, be ought today, English, the popular high-grade potted flower of Mei Deng state.Again because its nature florescence focuses mostly in 2 ~ April of spring, also become important on China flowers market in recent years and view and admire potted flower.Therefore, spring stem of noble dendrobium good variety selection is carried out and large-scale production all has wide market prospects.
In the world, Japan is main product state and the country of consumption of dendrobium nobile potted flower, and the production system of its spring stem of noble dendrobium is comparatively perfect, and forcing culture Technical comparing is ripe.Along with deepening continuously of spring stem of noble dendrobium breed of variety work sutdy, the different new varieties of genetic background continue to bring out, but there is not been reported about the research of spring stem of noble dendrobium improved seeds early screening.
Although start late for spring dendrobe cultivation and research domestic, scale, the factorial praluction of the spring stem of noble dendrobium are very fast.Some Enterprises, except Introduced From Abroad excellent spring stem of noble dendrobium kind, starts autonomous cultivation and researches and develops spring stem of noble dendrobium new varieties.But owing to being subject to the impact of the factors such as milpa weather conditions, breed difference, the one-tenth flower quality for new varieties can not ensure.Therefore, the short time selects the improved seeds that the cultivation of suitable China is produced, and is convenient to marketing.At present, the method that enterprise's many employings large area is planted experimentally carries out screening varieties to external introduced variety and independent research kind.Because the spring stem of noble dendrobium is perennial plant, the trophophase is longer, and Plant technique just can be bloomed for 3 years, and the screening cycle is long, and production cost is high.Therefore, the research carrying out spring stem of noble dendrobium improved seeds early screening is very necessary.
Summary of the invention
In order to solve the problem, the invention provides a kind of method of spring stem of noble dendrobium improved seeds early screening, comprising the following steps:
A. measure annual cuttage seeding morphological index: in spring stem of noble dendrobium cuttage seeding stop the leaf phase measure its plant height and leaf long;
B. calculate the spring stem of noble dendrobium and become flower quality integrate score: randomly draw the annual cuttage seeding being in the leaf phase of stopping, measure plant height and leaf long value, get average and substitute into regression equation y=0.511x
1+ 0.845x
2-7.247, calculate the spring stem of noble dendrobium and become flower quality integrate score y value, described x
1for plant height value, described x
2for leaf long value;
C. predict that the spring stem of noble dendrobium becomes flower quality grade: according to regression equation calculation gained y value prediction in step b, when becoming flower quality integrate score y >=0.21, become to spend best in quality; During-0.58 < y < 0.21, become flower quality medium; During y≤-0.58, become flower inferior quality.
D. the spring stem of noble dendrobium becomes the kind of flower quality integrate score y >-0.58, namely can be used for cultivation and produces.
Wherein, the leaf phase of stopping described in step a, the wide 0.4 ~ 0.6cm of leaf started, ripe gradually to the leaf that stops from the Ye Ye that stops long 2 ~ 3cm, and the long 4 ~ 5cm of the Ye Ye that stops, till the wide 1 ~ 1.5cm of leaf.
Wherein, in described step a, when the spring stem of noble dendrobium enters the leaf after date that stops, when down several 2nd leaf blade is about 5cm from top, carry out plant height and the long mensuration of leaf.
Wherein, in described step a, when the spring stem of noble dendrobium enters the leaf after date that stops, when down several 2nd leaf blade length is 4 ~ 6cm from top, carry out plant height and the long mensuration of leaf.
Wherein, plant height is the height of collar place to stem top; The long length for top blade of leaf.
Beneficial effect of the present invention:
The present invention establishes a kind of index system of spring stem of noble dendrobium improved seeds early screening, shortens the time limit of screening varieties, shortens to 1.5 years by 3 ~ 4 years, substantially increase the efficiency of screening varieties, reduce production cost.Simultaneously for other plant early screening provides the reference of theoretical foundation and method.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
If no special instructions, the kind related in embodiment is the commercially available kind of Beijing Sen Hezhong industry limited company.
Embodiment 1
Select into colored quality and have gradient disparities, there are extensively representational 6 spring stem of noble dendrobium Hybrids: improved seeds ' gloomy standing grain 2006 ' and ' gloomy standing grain H1 ', medium kind ' gloomy standing grain 2005 ' and ' gloomy standing grain 2007 ', poor kind ' gloomy standing grain 818 ' and ' gloomy standing grain 2002 ', to bloom plant full-bloom stage respectively at stop leaf phase and the corresponding lifes in 3 years of the annual cuttage seeding of the spring stem of noble dendrobium, carry out growth indexes and become flower index of quality mensuration, the data obtained all adopts Excel and SPSS V18.0 software to analyze.
Growth of cuttage seedlings index comprises that stem is thick, joint length, joint number, the number of sheets, leaf length, Ye Kuan, plant height, fresh weight, root are long, its assay method:
Fresh weight (g): not containing the weight of the whole cuttage plant of cuttings stipes.
Plant height (cm): for collar place is to the height on stem top, measures with meter ruler, and reading to be accurate to after decimal point 1.
Stem thick (cm): the mean value that two vertical direction stems of the thickest stipes of plant are thick.
Leaf long (cm): the leaf of top blade is long, with meter ruler measurement, reading to be accurate to after decimal point 1.
Leaf wide (cm): survey the width that the widest position of blade is decided to be blade.
Root long (cm): when every plant radical >=3, measure the length of 3 representational.
Each kind observes 30 strains respectively, gets average (see table 1).
Table 1 stops the upgrowth situation of leaf phase different spring stem of noble dendrobium kind cuttage seeding
Note: adopt Duncan multiple comparison graph, small English alphabet represents that the different spring stem of noble dendrobium becomes the significant difference of the flower index of quality in 0.05 level.Lower same
Become the flower index of quality be individual plant flowering rate, continuously bloom joint number, open 3 colored joint numbers, open 2 colored joint numbers and open 1 colored joint number, its assay method:
Individual plant flowering rate (%): (individual plant bloom the total joint number of joint number/individual plant) × 100%.
Joint number of blooming continuously (joint): the perpetual total joint number of plant.
Open 3 colored joint numbers (joint): plant often saves the joint number that 3 flowers are opened in position.
Open 2 colored joint numbers (joint): plant often saves the joint number that 2 flowers are opened in position.
Open 1 colored joint number (joint): plant often saves the joint number that 1 flower is opened in position.
Each kind is random observation 30 strain respectively, gets average (see table 2).
The situation of blooming of the different spring stem of noble dendrobium kind strain of table 2
Become flower quality quantitatively evaluating: utilize SPSS18.0 software kit to each kind individual plant flowering rate (X
1), joint number of blooming continuously (X
2), open 3 colored joint number (X
3), open 2 colored joint number (X
4), open 1 colored joint number (X
5) 5 indexs of blooming carry out principal component analysis, are extracted 2 principal component: F1=0.518ZX
1+ 0.251ZX
2-0.380ZX
3+ 0.641ZX
4+ 0.335ZX
5, F2=0.429ZX
1+ 0.632ZX
2+ 0.555ZX
3-0.100ZX
4-0.315ZX
5, its eigen value is respectively 2.184 and 2.045, explains total variance 84.57%.Using the eigen value corresponding to each principal component account for the ratio of the total eigen value sum of extraction principal component to obtain the collective model of principal component as weight calculation: F=0.475ZX1+0.435ZX2+0.072ZX3+0.283ZX4+0.021ZX5, wherein ZX1, ZX2,, ZX5 is the value of original variable through standardization.Substitute in collective model by after table 2 data normalization, obtain each kind and become the integrate score of flower quality to be followed successively by from high to low: ' the gloomy standing grain 2002 ' (-1.14) of gloomy standing grain 2006 ' (1.88) > ' gloomy standing grain H1 ' (0.21) > ' gloomy standing grain 2007 ' (-0.24) > ' gloomy standing grain 818 ' (-0.58) > '.Kind grade classification normative reference is: when becoming flower quality integrate score y >=0.21, become to spend best in quality; During-0.58 < y < 0.21, become flower quality medium; During y≤-0.58, become flower inferior quality.
Use bivariate Correlation analyses to carry out cuttage seeding to stop leaf phase growth indexes and the correlation analysis becoming colored quality integrate score, result (see table 3).
Table 3 stop leaf phase growth indexes and become flower quality correlation coefficient
Note: adopt Pearson correlation coefficient, two-sided criterion of significance, in * 0.05 level significantly; * remarkable in * 0.01 level.
Obtain with become the cuttage seeding of colored quality significant correlation stop leaf phase growth indexes be leaf long, save length, plant height, stem are thick.Utilize multiple linear regression method to carry out significance index of correlation and the regression analysis becoming flower quality integrate score, set up effective regression equation: y=0.511x
1(plant height)+0.845x
2(leaf is long)-7.247, correlation coefficient r=0.981, effectively can predict that the spring stem of noble dendrobium becomes flower quality integrate score.
Embodiment 2
When the annual cuttage seeding of the spring stem of noble dendrobium enters the leaf phase of stopping, when by top, down several 2nd leaf is about 5cm, ' gloomy standing grain 4001 ' (production being evaluated as poor kind) measures its leaf length, plant height (method is with embodiment 1) to random selecting 30 strain spring stem of noble dendrobium kind.The long leaf for top blade of leaf is long, and plant height is the height of collar place to stem top.
Get the average (cm) of two indices, x1=3.94, x2=5.49, substitute into regression equation y=0.511x1+0.845x2-7.247, obtain it and become flower quality integrate score y=-0.59 <-0.58, belong to poor kind, should not production be cultivated, above evaluate consistent with production.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (1)
1. a method for spring stem of noble dendrobium improved seeds early screening, comprises the following steps:
A. annual cuttage seeding morphological index is measured: when the spring stem of noble dendrobium enters the leaf after date that stops, when from top, down several 2nd leaf blade length is 4 ~ 6cm, carry out plant height and the long mensuration of leaf, the described leaf phase of stopping, the wide 0.4 ~ 0.6cm of leaf started from the Ye Ye that stops long 2 ~ 3cm, ripe gradually to the leaf that stops, long 4 ~ the 5cm of the Ye Ye that stops, till the wide 1 ~ 1.5cm of leaf, wherein, plant height is the height of collar place to stem top, the long length for top blade of leaf;
B. calculate the spring stem of noble dendrobium and become flower quality integrate score: randomly draw the annual cuttage seeding being in the leaf phase of stopping, measure plant height and leaf long value, get average and substitute into regression equation y=0.511x
1+ 0.845x
2-7.247, calculate the spring stem of noble dendrobium and become flower quality integrate score y value, described x
1for plant height value, described x
2for leaf long value;
C. predict that the spring stem of noble dendrobium becomes flower quality grade: according to regression equation calculation gained y value prediction in step b, when becoming flower quality integrate score y >=0.21, become to spend best in quality; During-0.58 < y < 0.21, become flower quality medium; During y≤-0.58, become flower inferior quality;
D. the spring stem of noble dendrobium becomes the kind of flower quality integrate score y >-0.58, namely can be used for cultivation and produces.
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| CN104542025B (en) * | 2014-12-01 | 2017-02-22 | 北京林业大学 | Early screening method for good varieties of bundle plant type nobile-type dendrobium |
| CN107067016B (en) * | 2016-11-10 | 2020-03-17 | 浙江农林大学 | Loquat endophyte classification method based on leaf color |
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| USPP16746P3 (en) * | 2004-09-17 | 2006-07-04 | Yuen Foong Yu Paper Mfg. Co., Ltd. | Dendrobium plant named ‘YFY-HS1’ |
| JP2010220596A (en) * | 2009-03-19 | 2010-10-07 | Nicchu Kokusai Tsusho Kk | Dendrobium growing downward |
| CN101750471A (en) * | 2009-12-17 | 2010-06-23 | 华中农业大学 | Method for fast screening dendrobium nobile potted flower with high quality |
| CN102928373B (en) * | 2012-11-26 | 2015-05-13 | 广东省农业科学院花卉研究所 | Method for testing content of dendrobe polysaccharide and application thereof |
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