CN107991299B - DNA sample detection system of IVD (in vitro-detection device) - Google Patents
DNA sample detection system of IVD (in vitro-detection device) Download PDFInfo
- Publication number
- CN107991299B CN107991299B CN201711128313.5A CN201711128313A CN107991299B CN 107991299 B CN107991299 B CN 107991299B CN 201711128313 A CN201711128313 A CN 201711128313A CN 107991299 B CN107991299 B CN 107991299B
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- China
- Prior art keywords
- light
- optical fiber
- ivd
- dna sample
- detection system
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- 238000001514 detection method Methods 0.000 title claims abstract description 37
- 239000013307 optical fiber Substances 0.000 claims abstract description 57
- 230000003287 optical effect Effects 0.000 claims abstract description 20
- 238000000338 in vitro Methods 0.000 claims abstract description 13
- 229910052736 halogen Inorganic materials 0.000 claims description 5
- 150000002367 halogens Chemical class 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 3
- 230000004544 DNA amplification Effects 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 abstract description 6
- 239000008280 blood Substances 0.000 abstract description 3
- 210000004369 blood Anatomy 0.000 abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 1
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
Abstract
The invention discloses a DNA sample detection system of IVD in-vitro detection equipment, which comprises two light sources, a photoelectric detector, a PC and an optical signal collection device; the photoelectric detector is electrically connected with the PC, and the optical signal collecting device comprises a top cover and a plurality of incident optical fibers which are arranged on the lower surface of the top cover at equal intervals in parallel; a plurality of light-emitting notches which are arranged at equal intervals are formed in each incident optical fiber, and a reaction test tube is correspondingly placed below each light-emitting notch; two ends of the incident optical fiber extend out of the top cover and are converged into two integrated optical fibers, and light inlets of the two integrated optical fibers and light sources on corresponding sides of the two integrated optical fibers are positioned on the same straight line; meanwhile, a receiving optical fiber is arranged on one side of the side wall of the bottom of the reaction test tube, and a light outlet of the receiving optical fiber is opposite to the photoelectric detector; the invention has the advantages of solving the DNA amplification detection of clinical specimens such as blood, body cavity liquid, wash-rinse liquid, hair, cells, living tissues and the like, along with more stable lighting and higher signal-to-noise ratio.
Description
Technical Field
The invention relates to a detection system, in particular to a DNA sample detection system of IVD in-vitro detection equipment.
Background
The biological polymerase chain reaction is a molecular biological technology for amplifying specific DNA fragments, can be regarded as special DNA replication outside organisms, has the greatest characteristics of PCR, can greatly increase trace DNA, and can react to a PC end in detail through optical fiber conduction and CCD imaging, but the lighting is unstable and the signal to noise is lower in the existing DNA amplification detection.
Disclosure of Invention
The invention aims at: the DNA sample detection system is used for solving the DNA amplification detection of clinical specimens such as blood, body cavity liquid, wash liquid, hair, cells, living tissues and the like, and is provided with IVD in vitro detection equipment with more stable lighting and higher signal to noise ratio.
The technical scheme of the invention is as follows: a DNA sample detection system of IVD in vitro detection equipment comprises two light sources, a photoelectric detector, a PC and an optical signal collection device; the photoelectric detector is electrically connected with the PC, and the optical signal collecting device comprises a top cover and a plurality of incident optical fibers which are arranged on the lower surface of the top cover at equal intervals in parallel;
a plurality of light-emitting notches which are arranged at equal intervals are formed in each incident optical fiber, and a reaction test tube is correspondingly placed below each light-emitting notch; two ends of the incident optical fiber extend out of the top cover and are converged into two integrated optical fibers, and light inlets of the two integrated optical fibers and light sources on corresponding sides of the two integrated optical fibers are positioned on the same straight line; meanwhile, a receiving optical fiber is arranged on one side of the side wall of the bottom of the reaction test tube, and a light outlet of the receiving optical fiber is opposite to the photoelectric detector.
As the preferable technical scheme, the light emergent notch is triangular, the depth is 0.1-0.5mm, and the included angle is 60-120 degrees.
As a preferable technical scheme, a first optical filter is arranged between the light inlets at the two ends of the incident optical fiber and the light source at the corresponding side of the incident optical fiber.
As an optimal technical scheme, a second optical filter is arranged between the light outlet of the receiving optical fiber and the photoelectric detector.
As a preferable technical scheme, the lower surface of the top cover is provided with optical fiber placing grooves which are longitudinally arranged, transversely arranged or grid-shaped arranged.
As a preferable technical scheme, the device further comprises a reaction plate, wherein the reaction test tube is arranged on the reaction plate.
As a preferable technical scheme, the light source is an LED lamp or a halogen lamp.
As a preferred embodiment, the photodetector is a charge-coupled device or a photomultiplier.
The working process of the invention is as follows:
the two light sources (halogen lamps or LED lamps) emit light and respectively cut off wavelengths through the first optical filters, then respectively emit light into two ends of an incident optical fiber, emit light into the top of a corresponding reaction test tube below through a light outlet notch of the incident optical fiber and conduct the light into the reaction test tube, the light transmitted in the reaction test tube is emitted into a receiving optical fiber on one side of the tube wall, the light passes through the second optical filters to cut off wavelengths, and then conduct the light to a charge coupler or a photomultiplier to collect signals, and then feed the signals back to a PC; finally, the DNA amplification change of the reagent in the reaction test tube is judged through the display of the PC end software.
The invention has the advantages that:
1. compared with the prior art, the invention is used for solving the problems of DNA amplification detection, protein detection, microfluidic detection, chemiluminescence detection, enzyme-free detection and the like of clinical specimens such as blood, body cavity liquid, wash liquid, hair, cells, living tissues and the like, and the invention emits light beams to the corresponding reaction test tubes below through the light-emitting incisions on the incident optical fibers, has better light intensity consistency, has lower requirement on the reaction volume of reagents in the reaction test tubes, can collect 5-100um, and has more stable lighting and higher signal-to-noise ratio.
Drawings
The invention is further described below with reference to the accompanying drawings and examples:
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a bottom view of the top cover and the fiber placement groove of the present invention arranged in a grid pattern;
FIG. 3 is a schematic illustration of an incident fiber in accordance with the present invention;
FIG. 4 is an enlarged view of a portion of I of FIG. 3 in accordance with the present invention;
wherein: 1 reaction test tube, 2 incident optical fiber, 21 light outlet notch, 3 receiving optical fiber, 4 light source, 5 photoelectric detector, 6PC, 7 optical filter, 8 optical filter, 9 optical fiber plate, 10 top cover, 11 optical fiber placing groove.
Detailed Description
Examples: referring to fig. 1, a DNA sample detection system of an IVD in vitro detection device includes two light sources 4, a photodetector 5, a pc 6, and an optical signal collecting device; the photoelectric detector 5 is electrically connected with the PC 6, and the optical signal collecting device comprises a top cover 10 and a plurality of incident optical fibers 2 which are arranged on the lower surface of the top cover 10 at equal intervals in parallel; a plurality of light outlet cut-outs 21 which are arranged at equal intervals are arranged on each incident optical fiber 2, and a reaction test tube 1 is correspondingly arranged below each light outlet cut-out 21; two ends of the incident optical fiber 2 extend out of the top cover 10 and are converged into two integrated optical fibers, and the light inlets of the two integrated optical fibers and the light sources 4 at the corresponding sides of the two integrated optical fibers are positioned on the same straight line; meanwhile, a receiving optical fiber 3 is arranged on one side of the side wall of the bottom of the reaction test tube 1, and a light outlet of the receiving optical fiber 3 is opposite to the photoelectric detector 5.
Referring to fig. 3 to 4, the light-emitting slit 21 of the present invention has a triangular shape with a depth X of 0.1 to 0.5mm and an included angle Y of 60 to 120 °.
The invention is provided with a first optical filter 7 between the light inlets at the two ends of the incident optical fiber 2 and the light source 4 at the corresponding side; and a second optical filter 8 is arranged between the light outlet of the receiving optical fiber 3 and the photoelectric detector 5.
The optical fiber placing grooves 11 are arranged on the lower surface of the top cover 10 in a grid-shaped arrangement (as shown in fig. 2), the incident optical fibers 2 in the optical fiber placing grooves are longitudinally arranged, the optical fiber placing grooves 11 can also be transversely or longitudinally arranged, and the corresponding incident optical fibers 2 are transversely or longitudinally arranged.
The invention further comprises a reaction plate 9, the reaction test tube 1 is arranged on the reaction plate 9, the light source 4 is an LED lamp or a halogen lamp, and the photoelectric detector 5 is a charge coupler or a photomultiplier for converting optical signals into electric signals.
The working process of the invention is as follows:
the two light sources 4 (halogen lamps or LED lamps) emit light and respectively cut off wavelengths through the first optical filters 7, then respectively enter two ends of the incident optical fiber 2, pass through the light outlet notch 21 of the incident optical fiber 2, then enter the top of the corresponding reaction cuvette 1 below, are conducted into the reaction cuvette 1, and the transmitted light in the reaction cuvette 1 enters the receiving optical fiber 3 on one side of the tube wall, and then pass through the second optical filters 8 to cut off wavelengths, and then are conducted to the charge coupler or the photomultiplier to collect signals, and then are fed back to the PC 6; finally, the DNA amplification change of the reagent 1 in the reaction test tube is judged through the display of the software at the 6 end of the PC.
The above embodiments are provided to illustrate the technical concept and features of the present invention and are intended to enable those skilled in the art to understand the content of the present invention and implement the same, and are not intended to limit the scope of the present invention. All equivalent changes or modifications made in accordance with the spirit of the present invention should be construed to be included in the scope of the present invention.
Claims (8)
1. The DNA sample detection system of the IVD in-vitro detection equipment comprises two light sources (4), a photoelectric detector (5), a PC (6) and an optical signal collecting device; the photoelectric detector (5) is electrically connected with the PC (6), and is characterized in that: the optical signal collecting device comprises a top cover (10) and a plurality of incidence optical fibers (2) which are arranged on the lower surface of the top cover (10) at equal intervals in parallel;
a plurality of light outlet notches (21) which are arranged at equal intervals are arranged on each incident optical fiber (2), and a reaction test tube (1) is correspondingly arranged below each light outlet notch (21); two ends of the incident optical fiber (2) extend out of the top cover (10) and are converged into two integrated optical fibers, and light inlets of the two integrated optical fibers and light sources (4) at corresponding sides of the two integrated optical fibers are positioned on the same straight line; meanwhile, a receiving optical fiber (3) is arranged on one side of the side wall of the bottom of the reaction test tube (1), and a light outlet of the receiving optical fiber (3) is opposite to the photoelectric detector (5).
2. The DNA sample detection system of an IVD in vitro detection device according to claim 1, wherein the light exit slit (21) is triangular with a depth of 0.1-0.5mm and an included angle of 60-120 °.
3. The DNA sample detection system of the IVD in vitro detection device according to claim 1, wherein a first optical filter (7) is arranged between the light inlets at both ends of the incident optical fiber (2) and the light source (4) at the corresponding side thereof.
4. The DNA sample detection system of an IVD in vitro detection device according to claim 1, wherein a second filter (8) is arranged between the light outlet of the receiving fiber (3) and the photodetector (5).
5. The DNA sample detection system of the IVD in vitro detection device according to claim 1, wherein the top cover (10) is provided with optical fiber placement grooves (11) arranged longitudinally, transversely or in a grid shape on the lower surface.
6. The DNA sample detection system of an IVD in vitro detection device according to claim 1, further comprising a reaction plate (9), wherein the reaction tube (1) is provided on the reaction plate (9).
7. The DNA sample detection system of an IVD in vitro detection device according to claim 1, wherein the light source (4) is an LED lamp or a halogen lamp.
8. The DNA sample detection system of an IVD in vitro detection device according to claim 1, wherein the photodetector (5) is a charge-coupled device or a photomultiplier tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711128313.5A CN107991299B (en) | 2017-11-15 | 2017-11-15 | DNA sample detection system of IVD (in vitro-detection device) |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711128313.5A CN107991299B (en) | 2017-11-15 | 2017-11-15 | DNA sample detection system of IVD (in vitro-detection device) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107991299A CN107991299A (en) | 2018-05-04 |
| CN107991299B true CN107991299B (en) | 2024-01-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711128313.5A Active CN107991299B (en) | 2017-11-15 | 2017-11-15 | DNA sample detection system of IVD (in vitro-detection device) |
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| Country | Link |
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| CN (1) | CN107991299B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104513796A (en) * | 2013-09-30 | 2015-04-15 | 王金鹤 | Novel detection device for thermal transmission in DNA amplification |
| CN105524826A (en) * | 2016-01-12 | 2016-04-27 | 天津喜诺生物医药有限公司 | Detection device of QPCR multichannel mobile light source |
| CN105675574A (en) * | 2016-03-17 | 2016-06-15 | 苏州天隆生物科技有限公司 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
| CN105980908A (en) * | 2014-02-12 | 2016-09-28 | 科磊股份有限公司 | Multi-spot scanning collection optics |
| CN207379941U (en) * | 2017-11-15 | 2018-05-18 | 苏州雅睿生物技术有限公司 | A kind of DNA sample detecting system of IVD vitro detections equipment |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7436569B2 (en) * | 2003-03-12 | 2008-10-14 | General Photonics Corporation | Polarization measurement and self-calibration based on multiple tunable optical polarization rotators |
| US9909984B2 (en) * | 2016-02-10 | 2018-03-06 | Tian Yang | Multichannel label-free biosensing optical-fiber system |
-
2017
- 2017-11-15 CN CN201711128313.5A patent/CN107991299B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104513796A (en) * | 2013-09-30 | 2015-04-15 | 王金鹤 | Novel detection device for thermal transmission in DNA amplification |
| CN105980908A (en) * | 2014-02-12 | 2016-09-28 | 科磊股份有限公司 | Multi-spot scanning collection optics |
| CN105524826A (en) * | 2016-01-12 | 2016-04-27 | 天津喜诺生物医药有限公司 | Detection device of QPCR multichannel mobile light source |
| CN105675574A (en) * | 2016-03-17 | 2016-06-15 | 苏州天隆生物科技有限公司 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
| CN207379941U (en) * | 2017-11-15 | 2018-05-18 | 苏州雅睿生物技术有限公司 | A kind of DNA sample detecting system of IVD vitro detections equipment |
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| CN107991299A (en) | 2018-05-04 |
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Address after: 215000 rooms 101 and 201, C7 building, biomedical industrial park, 218 Xinghu street, Suzhou Industrial Park, Jiangsu Province Applicant after: Suzhou Yarui Biotechnology Co.,Ltd. Address before: 215000 rooms 101 and 201, C7 building, bio nano technology park, 218 Xinghu street, Suzhou Industrial Park, Jiangsu Province Applicant before: SUZHOU MOLARRAY BIOTECHNOLOGY Co.,Ltd. |
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