CN105524826A - Detection device of QPCR multichannel mobile light source - Google Patents
Detection device of QPCR multichannel mobile light source Download PDFInfo
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- CN105524826A CN105524826A CN201610016732.9A CN201610016732A CN105524826A CN 105524826 A CN105524826 A CN 105524826A CN 201610016732 A CN201610016732 A CN 201610016732A CN 105524826 A CN105524826 A CN 105524826A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1861—Means for temperature control using radiation
Abstract
The invention relates to a detection device of a QPCR (Real-time Quantitative PCR Detecting System) multichannel mobile light source. The detection device comprises two upper rails and a rack belt, wherein a motor drives the rack belt so that a top beam plate slides along the upper rails; two rows of exciting light source assemblies are arranged on the top beam plate; a sample plate is fixedly arranged under the top beam plate; two rows of sample holes are formed in the sample plate; a sample pipe is arranged in each sample hole; a hot cover plate with a light passing hole is put on the top end of an upper opening of each sample pipe; by corresponding to each sample pipe, communicated transverse reflecting holes are formed in the outer side of each sample hole; monochromatic light given out by the exciting light source assemblies illuminates samples; and reflection light of the samples is emitted out through the reflection holes and is received by a signal receiving assembly arranged in an installing hole formed in the lower end of a side beam arm for realizing the imaging. The detection device has the advantages that the monochromatic light can be pure; the parasitic light interference is reduced; the exciting and sampling of a plurality of light sources can be performed on the samples simultaneously in the same time period; and the efficiency of a system is improved.
Description
Technical field
The invention belongs to technical field of medical detection, particularly a kind of proofing unit of QPCR hyperchannel mobile light source.
Background technology
The technology of QPCR is long-standing, and the Full Name in English of QPCR is Real-timeQuantitativePCRDetectingSystem.I.e. real time fluorescent quantitative nucleic acid amplification detection system, is also real-time quantitative gene amplification fluorescence detecting system, is called for short QPCR.Its equipment used is called real-time fluorescence quantitative PCR instrument, is exclusively used in this and detects.There is the ABI7x00 series QPCR instrument of AppliedBiosystems company of the U.S. in the producer wherein done well, the CFX96Touch quantitative fluorescent PCR instrument of Bio-rad company of the U.S., there are 7600 series reaching peace gene in domestic producer, Hangzhou An Jiesi, and China of section is biological.Their light-source system is divided into 2 large classes substantially, and first class is representative with external: halogen tungsten lamp-optical filtering-CCD scheme, and second largest class take domestic manufacturer as representative: LED-optical fiber-photo-sensor.First class is a kind of very ripe scheme abroad got through research and development for many years and experiment, simultaneously its advantage uses halogen tungsten lamp to impinge upon fluorescence excitation on sample by spectral filter, join spectral filter by CCD again to take a picture, draw numerical value by software analysis.The advantage of this mode gathers rapidly, disposablely can complete the even more sample of detection 96, and various combination of can arranging in pairs or groups, superior.Its shortcoming is that light path is complicated, owing to using CCD to produce fringing effect, must correct by regular calibration object.One sets of plan of manufacturer of second largest Lei Shi China research, carries out guide-lighting fluorescence excitation with LED cold light light source by optical fiber, then by optical fiber, fluorescence is imported to photo-sensor and read.This design avoids complicated light path design and the fringing effect of high-quality CCD, is also improve the work-ing life of light source by LED cold light source, but it is complicated that internal optical fiber can be brought to design, the problem that detection efficiency is low.Especially in multiple light courcess detects, the inferior position of this mode has just embodied.
Summary of the invention
The object of the invention is the technical problem occurred for the detection mode of domestic use, propose the easier detection scheme more efficiently of one, specifically propose a kind of proofing unit of QPCR hyperchannel mobile light source.
The present invention solves its technical problem and takes following technical scheme to realize:
A kind of proofing unit of QPCR hyperchannel mobile light source, comprise two levels get on the right track and be arranged on two get on the right track between horizontal teeth band, be raising-plate two belows of getting on the right track, above raising-plate, corner place is fixed with rolling pulley, in rolling pulley is installed on and gets on the right track, and along getting on the right track slip, the peg of head with draw-in groove is fixed with in the upper surface middle part of raising-plate, the draw-in groove of peg head and the clamping of tooth bar band, driven by motor tooth bar Tape movement, tooth bar band drives raising-plate along getting on the right track movement, the identical excitation light source assembly of two row's structures is installed in the both sides, middle part of raising-plate, the LED cold light source of excitation light source assembly is inlaid in raising-plate, transmitting spectral filter is fixedly mounted with in the below of LED cold light source, negative lens group is fixedly mounted with in the below of launching spectral filter, horizontal frock platen is had in the positioned beneath of raising-plate, frock platen is fixedly mounted with sample plane, sample plane is provided with this hole of two stock layouts, the spacing that the spacing and two in two these holes of stock layout arranges excitation light source assembly is identical, sample tube is placed in each sample hole, hot cover plate is placed with on the upper shed top of sample tube, hot cover plate is shaped with two rankings and puts the light hole corresponding with sample tube upper shed position, simultaneously, corresponding to each sample tube, sample plane outside each sample hole is shaped with the horizontal reflection hole of connection, the light be radiated on sample outwards launches sample reflection light by reflection hole, identical and the curb girder arm vertically downward of two structures is fixed with in the both sides of raising-plate, laterally row's open holes is shaped with in the lower end of each curb girder arm, the position of each open holes is corresponding with the position of reflection hole, receiver lens group is fixed with successively from inside to outside in each open holes, receive spectral filter and photo-sensor, the monochromatic ray of focusing illumination on sample excites sample to send fluorescence, fluorescence is through receiver lens group and receive spectral filter imaging on the photosensor.
And it is three that the excitation light source assembly that described two row's structures are identical is often arranged, and corresponding with excitation light source assembly, the open holes number that described each curb girder arm lower end is shaped with is three.
And the frock platen of described raising-plate positioned beneath is and gets on the right track relatively-stationary frock platen, and it is eight that this hole of two stock layouts that sample plane is arranged often is arranged.
And, in testing process, each raising-plate along movement of getting on the right track distance with often arrange in the spacing in two sample holes identical.
Advantage of the present invention and positively effect are:
1, the LED light source that the present invention uses is not transmitted by optical fiber, but by air and lens, avoid the loss of exciting light on optical fiber, and exciting light is focused on intensity sample adding exciting light by scioptics.All be furnished with spectral filter monochromatic ray can be made purer in the light path of exciting light and in the light path receiving light, reduce light disturbance.
2, the high sensitivity photosensors of the reception fluorescent signal of the present invention's use also does not go to import by optical fiber, but focuses on photo-sensor by air and lens by scattering fluorescence scioptics in the optical path, adds the intensity of signal.
3, the arrangement of sample arrangement of the present invention and exciting light and reception light can be carried out exciting of several light source to sample simultaneously and sample within some time periods, adds the efficiency of system.
Accompanying drawing explanation
Fig. 1 is main TV structure schematic diagram of the present invention;
Fig. 2 is the left TV structure schematic diagram of Fig. 1, wherein, by the curb girder arm section in Fig. 1 and the signal receiving assembly partial removal being arranged on curb girder arm bottom;
Fig. 3 is the left TV structure schematic diagram of curb girder arm in Fig. 1.
Embodiment
Be further described the invention process below, following examples are descriptive, are not determinate, can not limit protection scope of the present invention with this.
A kind of proofing unit of QPCR hyperchannel mobile light source, as shown in the figures 1 and 2, comprise two levels get on the right track 1 and be arranged on two get on the right track between horizontal teeth band 4, be raising-plate 5 in the below that two levels get on the right track, above raising-plate, corner place is fixed with rolling pulley 2, in rolling pulley is installed on and gets on the right track, and along getting on the right track slip, the peg 3 of head with draw-in groove is fixed with in the upper center of raising-plate, the draw-in groove of peg head and the clamping of tooth bar band, driven by motor tooth bar Tape movement, tooth bar band drives peg to move, peg drives raising-plate along getting on the right track movement, the identical excitation light source assembly of two row's structures is installed in the both sides, middle part of raising-plate, the LED cold light source 6 of excitation light source assembly is inlaid in raising-plate, be fixedly mounted with in the below of LED cold light source and launch spectral filter 7, negative lens group 8 is fixedly mounted with in the below of launching spectral filter, LED cold light source focuses on below monochromatic light directed after launching spectral filter and negative lens group, horizontal frock platen 16 is had in the positioned beneath of raising-plate, frock platen is fixed with sample plane 13, sample plane is provided with this hole of two stock layouts, the spacing that the spacing and two in two these holes of stock layout arranges excitation light source assembly is identical, sample tube 15 is placed in each sample hole, hot cover plate 17 is placed with on the upper shed top of sample tube, hot cover plate is shaped with two rankings and puts the light hole 18 corresponding with sample tube upper shed position, the monochromatic ray launched by each excitation light source assembly is through on the sample of light hole focusing illumination in sample tube, simultaneously, corresponding to each sample tube, sample plane outside each sample hole is shaped with the horizontal reflection hole 14 of connection, the light be radiated on sample outwards launches sample reflection light by reflection hole, as shown in Figure 1, identical and the curb girder arm 9 vertically downward of two structures is fixed with in the both sides of raising-plate, as shown in Figure 3, laterally row's open holes 20 is shaped with in the lower end of each curb girder arm, the position of each open holes is corresponding with the position of reflection hole, receiver lens group 10 is fixed with successively from inside to outside in each open holes, receive spectral filter 11 and photo-sensor 12, the monochromatic ray of focusing illumination in sample tube on sample passes through sample reflection, reflected light is through receiver lens group and receive spectral filter imaging on the photosensor.
In specific embodiment of the invention, it is three that the excitation light source assembly that described two row's structures are identical is often arranged, and corresponding with excitation light source assembly, the open holes number that described each curb girder arm lower end is shaped with is three.
In specific embodiment of the invention, the frock platen of the positioned beneath of described raising-plate is and gets on the right track relatively-stationary frock platen, and it is eight that this hole of two stock layouts that sample plane is arranged often is arranged, and the sample tube be namely placed in sample hole amounts to 16.
In specific embodiment of the invention, in testing process, each raising-plate along movement of getting on the right track distance with often arrange in the spacing in two sample holes identical, the sample plane be packed on frock platen is motionless, driven by motor tooth bar band moves using the spacing in two sample holes as step units, and namely the sample that correspondence is arranged will irradiate once by each LED cold light source, and whole raising-plate wants stepping ten times, meanwhile, each sample respectively can be irradiated once by different LED cold light sources.
In specific embodiment of the invention, the side, front and back end of described raising-plate both sides has three bolts hole 19 arranged in a triangle, curb girder arm is by being installed on the both sides being bolted to raising-plate on bolt hole.
The principle of work of apparatus of the present invention
During experiment, whole mechanism runs like this, the excitation light source assembly linked and the signal receiving assembly be made up of receiver lens group, reception spectral filter and photo-sensor all can move to the left side of sample portion, all excitation light source assemblies and signal receiving assembly all can not detect the sample of eight from left to right, when detecting beginning, whole link gear can move right
First, the excitation light source assembly of low order end and the signal receiving assembly of low order end will detect the sample of high order end,
Then, excitation light source assembly and the signal receiving assembly of interlock move using the spacing in two sample holes as step units, successively sample is from left to right detected, until the excitation light source assembly of high order end and the signal receiving assembly of high order end move on on the right side of the sample of low order end, detect and terminate.
The advantage of such detection mode is:
1, in the middle of detection, wherein there are 4 steps to detect by 3 passages simultaneously simultaneously, wherein have 4 steps to detect by 2 passages simultaneously.The detection completing 3 passages needs 13 steps, and traditional optical fiber solutions then needs 24 steps just can complete, and such scheme accelerates 45% on detection speed.
2, as shown in Figure 1, there are 2 identical proofing units in whole mechanism, and be symmetrical detection, therefore efficiency can also double.
Claims (4)
1. the proofing unit of a QPCR hyperchannel mobile light source, it is characterized in that: comprise two levels get on the right track and be arranged on two get on the right track between horizontal teeth band, be raising-plate two belows of getting on the right track, above raising-plate, corner place is fixed with rolling pulley, in rolling pulley is installed on and gets on the right track, and along getting on the right track slip, the peg of head with draw-in groove is fixed with in the upper surface middle part of raising-plate, the draw-in groove of peg head and the clamping of tooth bar band, driven by motor tooth bar Tape movement, tooth bar band drives raising-plate along getting on the right track movement, the identical excitation light source assembly of two row's structures is installed in the both sides, middle part of raising-plate, the LED cold light source of excitation light source assembly is inlaid in raising-plate, transmitting spectral filter is fixedly mounted with in the below of LED cold light source, negative lens group is fixedly mounted with in the below of launching spectral filter, horizontal frock platen is had in the positioned beneath of raising-plate, frock platen is fixedly mounted with sample plane, sample plane is provided with this hole of two stock layouts, the spacing that the spacing and two in two these holes of stock layout arranges excitation light source assembly is identical, sample tube is placed in each sample hole, hot cover plate is placed with on the upper shed top of sample tube, hot cover plate is shaped with two rankings and puts the light hole corresponding with sample tube upper shed position, simultaneously, corresponding to each sample tube, sample plane outside each sample hole is shaped with the horizontal reflection hole of connection, the light be radiated on sample outwards launches sample reflection light by reflection hole, identical and the curb girder arm vertically downward of two structures is fixed with in the both sides of raising-plate, laterally row's open holes is shaped with in the lower end of each curb girder arm, the position of each open holes is corresponding with the position of reflection hole, receiver lens group is fixed with successively from inside to outside in each open holes, receive spectral filter and photo-sensor, the monochromatic ray of focusing illumination on sample excites sample to send fluorescence, fluorescence is through receiver lens group and receive spectral filter imaging on the photosensor.
2. the proofing unit of QPCR hyperchannel mobile light source according to claim 1, it is characterized in that: it is three that the excitation light source assembly that described two row's structures are identical is often arranged, corresponding with excitation light source assembly, the open holes number that described each curb girder arm lower end is shaped with is three.
3. the proofing unit of QPCR hyperchannel mobile light source according to claim 1, is characterized in that: the frock platen of described raising-plate positioned beneath is and gets on the right track relatively-stationary frock platen, and it is eight that this hole of two stock layouts that sample plane is arranged often is arranged.
4. the proofing unit of QPCR hyperchannel mobile light source according to claim 1, is characterized in that: in testing process, each raising-plate along movement of getting on the right track distance with often arrange in the spacing in two sample holes identical.
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CN201610016732.9A CN105524826A (en) | 2016-01-12 | 2016-01-12 | Detection device of QPCR multichannel mobile light source |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107746806A (en) * | 2017-11-20 | 2018-03-02 | 鲲鹏基因(北京)科技有限责任公司 | A kind of real-time fluorescence quantitative PCR instrument |
CN107991299A (en) * | 2017-11-15 | 2018-05-04 | 苏州雅睿生物技术有限公司 | A kind of DNA sample detecting system of IVD vitro detections equipment |
CN110042150A (en) * | 2018-01-16 | 2019-07-23 | 青岛益柏生物科技有限公司 | A kind of real-time analytical equipment of nucleic acid constant-temperature amplification |
CN110132909A (en) * | 2018-02-08 | 2019-08-16 | 台达电子国际(新加坡)私人有限公司 | Fluorescence detection device |
CN112730369A (en) * | 2021-01-25 | 2021-04-30 | 苏州市中心血站 | Multichannel fluorescence detection data processing method |
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CN2766238Y (en) * | 2004-12-07 | 2006-03-22 | 中山大学达安基因股份有限公司 | Real-time fluorescence detecting device for nucleic acid amplification |
CN105092543A (en) * | 2014-05-12 | 2015-11-25 | 绍兴安尼特微电子科技有限公司 | Portable fluorescence quantitative PCR detector |
CN205368371U (en) * | 2016-01-12 | 2016-07-06 | 天津喜诺生物医药有限公司 | Detection apparatus for QPCR multichannel removes light source |
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Patent Citations (4)
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CN1379236A (en) * | 2001-04-12 | 2002-11-13 | 杭州大和热磁电子有限公司 | Fluorescence quantitative PCR analyzing system |
CN2766238Y (en) * | 2004-12-07 | 2006-03-22 | 中山大学达安基因股份有限公司 | Real-time fluorescence detecting device for nucleic acid amplification |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107991299A (en) * | 2017-11-15 | 2018-05-04 | 苏州雅睿生物技术有限公司 | A kind of DNA sample detecting system of IVD vitro detections equipment |
CN107991299B (en) * | 2017-11-15 | 2024-01-30 | 苏州雅睿生物技术股份有限公司 | DNA sample detection system of IVD (in vitro-detection device) |
CN107746806A (en) * | 2017-11-20 | 2018-03-02 | 鲲鹏基因(北京)科技有限责任公司 | A kind of real-time fluorescence quantitative PCR instrument |
CN107746806B (en) * | 2017-11-20 | 2024-02-20 | 鲲鹏基因(北京)科技有限责任公司 | Real-time fluorescence quantitative PCR instrument |
CN110042150A (en) * | 2018-01-16 | 2019-07-23 | 青岛益柏生物科技有限公司 | A kind of real-time analytical equipment of nucleic acid constant-temperature amplification |
CN110132909A (en) * | 2018-02-08 | 2019-08-16 | 台达电子国际(新加坡)私人有限公司 | Fluorescence detection device |
CN112730369A (en) * | 2021-01-25 | 2021-04-30 | 苏州市中心血站 | Multichannel fluorescence detection data processing method |
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