CN107974407B - Simple device for efficiently amplifying peripheral blood NK cells - Google Patents
Simple device for efficiently amplifying peripheral blood NK cells Download PDFInfo
- Publication number
- CN107974407B CN107974407B CN201711290960.6A CN201711290960A CN107974407B CN 107974407 B CN107974407 B CN 107974407B CN 201711290960 A CN201711290960 A CN 201711290960A CN 107974407 B CN107974407 B CN 107974407B
- Authority
- CN
- China
- Prior art keywords
- box body
- culture
- plate
- mounting plate
- peripheral blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a simple device for efficiently amplifying peripheral blood NK cells, which comprises a box body, wherein the top end of the box body is an opening end of the box body, a sealing plate is inserted into the top end of the inner wall of the box body in an inserting manner, the upper surface of the sealing plate is fixedly connected with the lower surface of a box cover, the lower surface of the box cover is also contacted with the top end of the box body, the middle part of the lower surface of the sealing plate is fixedly connected with the top end of an installation plate, a slot is formed in the position, corresponding to the installation plate, of the inner bottom wall of. This simple and easy device of high-efficient augmentation peripheral blood NK cell all from last three culture plate to the three culture plate of fixedly connected with in proper order down through the side about the mounting panel, can the inside space of effectual utilization box body to help the contact of cell and box body culture solution in the culture plate, utilize the characteristic of NK cell happiness, use the cultivation lamp for the NK cell light filling, accelerate its amplification rate, solved the problem that proposes in the background art.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a simple device for efficiently amplifying peripheral blood NK cells.
Background
NK cells, also known as natural killer cells, natural killer cells are important immune cells in the body, are not only related to anti-tumor, anti-viral infection and immune regulation, but also participate in the occurrence of hypersensitivity and autoimmune diseases under certain conditions, and can recognize target cells and killing mediators.
At present, the amplification culture of NK cells is mostly carried out in a simple culture dish, the amplification speed of the NK cells is limited when the culture device is used for the NK cells, and the increasing requirements of the current medical field cannot be met, so that a culture device capable of improving the amplification speed of the NK cells is urgently needed.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a simple device for efficiently amplifying peripheral blood NK cells, and solves the problems in the prior art.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a simple device for efficiently amplifying peripheral blood NK cells comprises a box body, wherein the top end of the box body is an open end, a sealing plate is inserted into the top end of the inner wall of the box body, the upper surface of the sealing plate is fixedly connected with the lower surface of a box cover, the lower surface of the box cover is also contacted with the top end of the box body, the middle part of the lower surface of the sealing plate is fixedly connected with the top end of a mounting plate, the bottom wall of the box body is provided with an inserting groove corresponding to the position of the mounting plate, the bottom end of the mounting plate is inserted into the inserting groove, the left side surface and the right side surface of the mounting plate are respectively and fixedly connected with three culture plates from top to bottom, the middle part of each culture plate is provided with a culture tank, the culture plates are also provided with liquid leakage holes at the peripheries of the culture tanks, the bottom and the venthole intercommunication of inlet port, the inlet port is located its top, the through-hole has all been seted up to the position that corresponds the inlet port on shrouding and the lid, inlet port and through-hole intercommunication, the last fixed surface of lid installs the air pump, the end of giving vent to anger of air pump and the fixed intercommunication in top of through-hole, the upper surface of lid just is located the fixed intercommunication in right side of air pump has the inlet end of outlet duct, and the inlet end of outlet duct run through the shrouding and communicate with the inside of box body, the inlet end of check valve is fixed to the end of giving vent to anger of outlet duct, the equal fixed mounting in side has the.
Preferably, the number of the liquid leakage holes is eight, and the eight liquid leakage holes are arranged in an arc shape by taking the middle part of the culture tank as a center.
Preferably, the mounting panel is the ya keli board, the box body is the ya keli box body.
Preferably, the light emitting ends of the two cultivation lamps point to the inside of the box body.
Preferably, the three culture plates on the same side of the mounting plate are parallel to each other, and four sides of the sealing plate are respectively contacted with four side walls of the box body.
Preferably, the air outlet is located below the culture plate at the lowest end of the side surface of the mounting plate.
(III) advantageous effects
The invention provides a simple device for efficiently amplifying peripheral blood NK cells, which has the following beneficial effects:
(1) this simple and easy device of high-efficient augmentation peripheral blood NK cell all from last three culture plate to the three culture plate of fixedly connected with in proper order down through the side about the mounting panel, can the inside space of effectual utilization box body to help the contact of cell and box body culture solution in the culture plate, utilize the characteristic of NK cell happiness light, use the cultivation lamp for the NK cell light filling, accelerate its amplification rate, solved the problem that proposes in the background art.
(2) This simple and easy device of high-efficient augmentation peripheral blood NK cell, the inlet port has been seted up on through the mounting panel, the bottom and the venthole intercommunication of inlet port, the inlet port is located its top, the last fixed surface of lid installs the air pump, the end of giving vent to anger of air pump and the fixed intercommunication in top of through-hole, can utilize the air pump to let in gas to the box body is inside, utilize gaseous drive culture solution motion, improve the contact of culture solution and NK cell, and then effectual help NK cell acquires the nutrient, improve its amplification rate, the problem of proposing in the background art has been solved.
Drawings
FIG. 1 is a front view of the structure of the present invention;
FIG. 2 is a front cross-sectional view of the structure of the present invention;
FIG. 3 is a schematic view of the structure of the plate of the present invention.
In the figure: the culture box comprises a box body 1, a sealing plate 2, a box cover 3, a mounting plate 4, a slot 5, a culture plate 6, a culture tank 7, a liquid leakage hole 8, an air outlet hole 9, an air inlet hole 10, a through hole 11, an air pump 12, an air outlet pipe 13, a check valve 14 and a culture lamp 15.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: a simple device for efficiently amplifying peripheral blood NK cells comprises a box body 1, wherein the top end of the box body 1 is an open end, a sealing plate 2 is inserted into the top end of the inner wall of the box body 1, the upper surface of the sealing plate 2 is fixedly connected with the lower surface of a box cover 3, the lower surface of the box cover 3 is also contacted with the top end of the box body 1, the middle part of the lower surface of the sealing plate 2 is fixedly connected with the top end of a mounting plate 4, a slot 5 is formed in the position, corresponding to the mounting plate 4, of the inner bottom wall of the box body 1, a mounting plate 4 is an acrylic plate, light emitted by a culture lamp 15 penetrates through the box body 1 and the mounting plate 4 to be contacted with cells, cell amplification is accelerated, the box body 1 is an acrylic box body 1, the bottom end of the mounting plate 4 is inserted into the slot 5, three culture plates 6 are fixedly connected to the left side surface and the right, four side surfaces of the sealing plate 2 are respectively contacted with four side walls of the box body 1, liquid leakage holes 8 are also formed in the periphery of the culture tank 7 on the culture plate 6, the number of the liquid leakage holes 8 is eight, the eight liquid leakage holes 8 are arranged in an arc shape by taking the middle part of the culture tank 7 as the center, when the mounting plate 4 is stretched into the box body 1, the mounting plate 4 is contacted with the culture solution, the culture solution firstly enters the upper part of the culture plate 6 through the liquid leakage holes 8 and then flows into the culture tank 7, NK cells in the culture tank can be prevented from being dispersed by the culture solution, the occurrence of sedimentation can be avoided, the bottom end of the mounting plate 4 is provided with an air outlet hole 9, the air outlet hole 9 is positioned below the culture plate 6 at the lowest end of the side surface of the mounting plate 4, two ends of the air outlet hole 9 are respectively positioned at the left side and the right side of the mounting plate 4, the mounting plate 4 is, the positions of the sealing plate 2 and the box cover 3 corresponding to the air inlet holes 10 are respectively provided with a through hole 11, the air inlet holes 10 are communicated with the through holes 11, the upper surface of the box cover 3 is fixedly provided with an air pump 12, the air outlet end of the air pump 12 is fixedly communicated with the top end of the through hole 11, the upper surface of the box cover 3 is fixedly communicated with the air inlet end of an air outlet pipe 13 positioned at the right side of the air pump 12, air can be introduced into the box body 1 by the air pump 12, the culture solution is driven to move by the air, the contact between the culture solution and NK cells is improved, further, the NK cells are effectively helped to obtain nutrients, the amplification speed of the NK cells is improved, the problem provided in the background technology is solved, the mixed gas of carbon dioxide and oxygen is used when the air is supplied by the air pump 12, the air is flushed into the culture solution in the box body 1 at a low, do not do too much and describe, and the inlet end of outlet duct 13 run through shrouding 2 and communicate with the inside of box body 1, the inlet end of the fixed mounting of outlet duct 13 has check valve 14, the equal fixed mounting in side has cultivation lamp 15 about box body 1, the inside of the equal directional box body 1 of the end of giving out light of two cultivation lamps 15 utilizes the characteristic of NK cell light preference, uses cultivation lamp 15 for the NK cell light filling for its amplification speed, has solved the problem of proposing in the background art.
During the use, the user injects the culture solution into the inside of box body 1 earlier, the cell raw materials that will need to cultivate are placed in culture tank 7, cover 3 lid at box body 1 again, make mounting panel 4 slowly stretch into inside the box body 1, and make in mounting panel 4's bottom insert slot 5, in-process culture solution leaks to go into on the culture plate 6 by weeping hole 8 and cultivates the raw materials contact with the cell in 7, open and cultivate 15 light supplementations of lamp, start air pump 12 to the inside mixed gas who injects carbon dioxide and oxygen of box body 1, stir the culture solution, improve the mixture of culture solution and cell.
To sum up, this simple and easy device of high-efficient expanded peripheral blood NK cell all from last three culture plate 6 of fixedly connected with down in proper order through the left and right sides of mounting panel 4, can the inside space of effectual utilization box body 1 to help the contact of culture plate 6 inner cell and the interior culture solution of box body 1, utilize the characteristic of NK cell light preference, use culture lamp 15 for the NK cell light filling, accelerate its speed of amplification, solved the problem that proposes in the background art.
This simple and easy device of high-efficient augmentation peripheral blood NK cell, inlet port 10 has been seted up on through mounting panel 4, the bottom and the venthole 9 intercommunication of inlet port 10, inlet port 10 is located its top, the last fixed surface of lid 3 installs air pump 12, the end of giving vent to anger of air pump 12 and the fixed intercommunication in top of through-hole 11, can utilize air pump 12 to let in gas to 1 inside of box body, utilize gaseous drive culture solution motion, improve the contact of culture solution and NK cell, and then effectual help NK cell acquires the nutrient, improve its amplification speed, the problem of proposing in the background art has been solved.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation. The electrical components presented in the document are all electrically connected with an external master controller and 220V mains, and the master controller can be a conventional known device controlled by a computer or the like.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. The utility model provides a simple and easy device of high-efficient amplification peripheral blood NK cell, includes box body (1), and the top of box body (1) is its open end, its characterized in that: the top end of the inner wall of the box body (1) is spliced with a sealing plate (2), the upper surface of the sealing plate (2) is fixedly connected with the lower surface of a box cover (3), the lower surface of the box cover (3) is also contacted with the top end of the box body (1), the middle part of the lower surface of the sealing plate (2) is fixedly connected with the top end of a mounting plate (4), a slot (5) is arranged at the position of the inner bottom wall of the box body (1) corresponding to the mounting plate (4), the bottom end of the mounting plate (4) is spliced in the slot (5), the left side surface and the right side surface of the mounting plate (4) are fixedly connected with three culture plates (6) from top to bottom in sequence, a culture tank (7) is arranged at the middle part of the culture plate (6), a liquid leakage hole (8) is also arranged on the periphery of the culture tank (7) on the culture, the two ends of the air outlet hole (9) are respectively positioned at the left side and the right side of the mounting plate (4), the mounting plate (4) is provided with an air inlet hole (10), the bottom end of the air inlet hole (10) is communicated with the air outlet hole (9), the air inlet hole (10) is positioned at the top end of the mounting plate, the positions of the sealing plate (2) and the box cover (3) corresponding to the air inlet hole (10) are respectively provided with a through hole (11), the air inlet hole (10) is communicated with the through hole (11), the upper surface of the box cover (3) is fixedly provided with an air pump (12), the air outlet end of the air pump (12) is fixedly communicated with the top end of the through hole (11), the upper surface of the box cover (3) is positioned at the right side of the air pump (12) and is fixedly communicated with the air inlet end of the air outlet pipe (13), the air inlet end of the air outlet pipe (13) penetrates through the sealing plate (2) and, the left side surface and the right side surface of the box body (1) are fixedly provided with culture lamps (15);
the number of the liquid leakage holes (8) is eight, and the eight liquid leakage holes (8) are arranged in an arc shape by taking the middle part of the culture tank (7) as the center.
2. The simple device for efficiently amplifying peripheral blood NK cells according to claim 1, wherein: the mounting panel (4) is the ya keli board, box body (1) is ya keli box body (1).
3. The simple device for efficiently amplifying peripheral blood NK cells according to claim 1, wherein: the light emitting ends of the two culture lamps (15) point to the inside of the box body (1).
4. The simple device for efficiently amplifying peripheral blood NK cells according to claim 1, wherein: three culture plates (6) that lie in same side on mounting panel (4) are parallel to each other, four sides of shrouding (2) contact with four lateral walls of box body (1) respectively.
5. The simple device for efficiently amplifying peripheral blood NK cells according to claim 1, wherein: the air outlet (9) is positioned below the culture plate (6) at the lowest end of the side surface of the mounting plate (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711290960.6A CN107974407B (en) | 2017-12-08 | 2017-12-08 | Simple device for efficiently amplifying peripheral blood NK cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711290960.6A CN107974407B (en) | 2017-12-08 | 2017-12-08 | Simple device for efficiently amplifying peripheral blood NK cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107974407A CN107974407A (en) | 2018-05-01 |
| CN107974407B true CN107974407B (en) | 2021-05-04 |
Family
ID=62009789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711290960.6A Active CN107974407B (en) | 2017-12-08 | 2017-12-08 | Simple device for efficiently amplifying peripheral blood NK cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107974407B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6562616B1 (en) * | 1999-06-21 | 2003-05-13 | The General Hospital Corporation | Methods and devices for cell culturing and organ assist systems |
| JP4194236B2 (en) * | 2000-11-21 | 2008-12-10 | 独立行政法人 宇宙航空研究開発機構 | Cell culture vessel |
| KR101287690B1 (en) * | 2012-02-15 | 2013-07-24 | 동국대학교 산학협력단 | Cell damage modeling device using microfluidic emement and cell damage modeling method using the same |
| JP2015133926A (en) * | 2014-01-17 | 2015-07-27 | 株式会社Ihi | Cell culture apparatus |
| CN204752745U (en) * | 2015-07-09 | 2015-11-11 | 广州齐志生物工程设备有限公司 | Breather pipe for culturing animal cells bioreactor |
| CN205528845U (en) * | 2016-04-29 | 2016-08-31 | 宁波市瑞德曼医疗投资管理有限公司 | High -efficient device that amplifys human NK cell |
| CN106929416A (en) * | 2017-05-04 | 2017-07-07 | 成都迈瑞斯特装饰材料有限公司 | A kind of teaching cell culture apparatus |
| CN206319012U (en) * | 2016-12-27 | 2017-07-11 | 北京弘润天源生物技术股份有限公司 | A kind of high cell culture incubator of sealing property |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8956860B2 (en) * | 2009-12-08 | 2015-02-17 | Juan F. Vera | Methods of cell culture for adoptive cell therapy |
| CN205893230U (en) * | 2016-06-29 | 2017-01-18 | 天津东方同创生物科技有限公司 | Cell culture board storage rack |
-
2017
- 2017-12-08 CN CN201711290960.6A patent/CN107974407B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6562616B1 (en) * | 1999-06-21 | 2003-05-13 | The General Hospital Corporation | Methods and devices for cell culturing and organ assist systems |
| JP4194236B2 (en) * | 2000-11-21 | 2008-12-10 | 独立行政法人 宇宙航空研究開発機構 | Cell culture vessel |
| KR101287690B1 (en) * | 2012-02-15 | 2013-07-24 | 동국대학교 산학협력단 | Cell damage modeling device using microfluidic emement and cell damage modeling method using the same |
| JP2015133926A (en) * | 2014-01-17 | 2015-07-27 | 株式会社Ihi | Cell culture apparatus |
| CN204752745U (en) * | 2015-07-09 | 2015-11-11 | 广州齐志生物工程设备有限公司 | Breather pipe for culturing animal cells bioreactor |
| CN205528845U (en) * | 2016-04-29 | 2016-08-31 | 宁波市瑞德曼医疗投资管理有限公司 | High -efficient device that amplifys human NK cell |
| CN206319012U (en) * | 2016-12-27 | 2017-07-11 | 北京弘润天源生物技术股份有限公司 | A kind of high cell culture incubator of sealing property |
| CN106929416A (en) * | 2017-05-04 | 2017-07-07 | 成都迈瑞斯特装饰材料有限公司 | A kind of teaching cell culture apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107974407A (en) | 2018-05-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203757870U (en) | Indoor oxygenation device | |
| CN213073934U (en) | Plant sugar-free culture system and plant culture container | |
| CN104988059B (en) | A kind of bioreactor for algal culture | |
| CN107974407B (en) | Simple device for efficiently amplifying peripheral blood NK cells | |
| CN213357608U (en) | Incubator for cell culture | |
| CN203668367U (en) | Microalgae photobioreactor | |
| CN107557290B (en) | Continuous culture apparatus for micro algae | |
| CN207567211U (en) | A kind of vertical rotary microalgae culturing device adopting | |
| CN205284506U (en) | Soilless culture device | |
| AU2019100892A4 (en) | A culture system for the production of microalgae active cell nutrition solution | |
| CN207468647U (en) | A kind of isolated carbon dioxide incubator of comparison | |
| CN206457493U (en) | A kind of pocket type anoxic box | |
| CN203247261U (en) | Microorganism culturing device | |
| CN207405171U (en) | A kind of culture apparatus for microorganism detection | |
| CN219586069U (en) | Fungus culture apparatus is used in dermatological inspection | |
| CN213142037U (en) | Quick culture apparatus of sewage treatment microorganism | |
| CN211394502U (en) | Culture apparatus for cell experiments | |
| CN215012198U (en) | Artificial intelligent temperature control device for edible fungi | |
| CN209602527U (en) | A kind of cell superclean bench | |
| CN203569085U (en) | Oil-producing microalgae culture device | |
| CN213388602U (en) | Little algae culture rack | |
| CN220643145U (en) | Natural killer cell culture and detection device | |
| CN206591107U (en) | A kind of teaching microbial cultivation device | |
| CN218897624U (en) | Carbon dioxide supply system for crop production | |
| CN210809050U (en) | Dough fermenting installation with exhaust treatment device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220512 Address after: 264003 No. 39 science and technology Avenue, hi tech Zone, Yantai, Shandong Patentee after: Shandong sileji Pharmaceutical Technology Co.,Ltd. Address before: 264003 No. 346, Guanhai Road, Yantai City, Shandong Province Patentee before: BINZHOU MEDICAL University |