CN220643145U - Natural killer cell culture and detection device - Google Patents

Abstract

The utility model discloses a natural killer cell culture and detection device, which relates to the technical field of cell culture and comprises an outer box and a detection box, wherein an incubator is arranged in the outer box, a temperature adjusting cavity is formed between the outer side wall of the incubator and the inner side wall of the outer box, a cell placement box and a culture solution placement box are respectively and fixedly arranged on the upper surface of the outer box and on the left side and the right side of a transparent glass plate, a culture tank is arranged below the inner part of the incubator, and a temperature regulator is fixedly arranged on the bottom surface of the outer box. The device places case, first catheter, culture solution and places case, second catheter and motorised valve through the cell and comes to direct into the incubator respectively natural killer cell and culture solution in, avoids the operator to frequently open the incubator and operates, influences the problem that the cell normally developed, controls the temperature of the inside water of chamber that adjusts the temperature through temperature regulator, heating pipe and refrigeration pipe simultaneously, adjusts to the temperature that natural killer cell is suitable for developing.

Description

Natural killer cell culture and detection device

Technical Field

The utility model relates to the technical field of cell culture, in particular to a natural killer cell culture and detection device.

Background

Natural killer cells are important immune cells of the organism, are not only related to anti-tumor, anti-viral infection and immune regulation, but also participate in the occurrence of hypersensitivity and autoimmune diseases in some cases, and can identify target cells and killing media.

The natural killer cells are generally used for carrying out immunotherapy on a human body, a large amount of natural killer cells with higher killing activity need to be expanded in vitro before the therapy, and the activity of the natural killer cells is detected, but the natural killer cell culturing device used in the market does not have the function of detecting the cells, the function is single, and when a culture solution is added to the cells, the culture box needs to be opened for operation, so that external microorganisms are easy to enter the culture box, the normal development of the cells is influenced, and therefore, the natural killer cell culturing and detecting device is provided for solving the defects.

Disclosure of Invention

In order to solve the technical problems, the technical scheme provides a natural killer cell culture and detection device, and solves the problems that the natural killer cell culture device used in the current market, which is proposed in the background art, does not have the function of detecting cells, has single function, and can be operated only by opening an incubator when adding culture solution to the cells, and is easy to cause external microorganisms to enter the incubator, thereby influencing the normal development of the cells.

In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a natural killer cell culture and detection device, includes outer box and detection case, the inside of outer box is provided with the incubator, form the chamber that adjusts the temperature between incubator lateral wall and the inside wall of outer box, the upper surface of outer box and be located transparent glass plate's left and right sides respectively fixed mounting have cell to place case and culture solution to place the case, the inside below of incubator is provided with the culture tank, the bottom surface fixed mounting of outer box has temperature regulator, temperature regulator's top both sides are provided with heating pipe and refrigeration pipe respectively, the work end of heating pipe and refrigeration pipe all runs through the bottom surface of outer box and extends to the inside in chamber that adjusts the temperature, the left side fixed mounting of outer box has the filter, the left side of filter is provided with the air pump, the output fixedly connected with breather pipe of filter, the inside rear side fixed mounting of incubator has the moisture machine, moisture machine and external control terminal electric connection, the detection case sets up on the right side of outer box, the equal fixedly connected with second stabilizer blade of the bottom surface both corners position department of detection case, the inside of detection case has the inside of placing the detection case to place the ware, the detection case is provided with the below the detection case.

Optionally, the bottom surface four corners position department of outer case all fixedly connected with first stabilizer blade, the upper surface of outer case is provided with transparent glass board.

Optionally, the top of case is placed to the cell is provided with first case lid, the bottom fixedly connected with first catheter of case is placed to the cell, the one end that the case was placed to the cell is kept away from to first catheter runs through the upper surface of outer case and extends to the inside of incubator, the inside of case is placed to the cell communicates with the inside of incubator through first catheter.

Optionally, the top of case is placed to the culture solution is provided with the second case lid, the bottom fixedly connected with second catheter of case is placed to the culture solution, the one end that the case was placed to the culture solution is kept away from to the second catheter runs through the upper surface of outer case and extends to the inside of incubator, the inside of case is placed to the culture solution communicates with the inside of incubator through the second catheter.

Optionally, the first catheter and the second catheter are provided with electric valves inside.

Optionally, the culture tank sets up the below at first catheter and second catheter, the right side of culture tank is by lower position department fixedly connected with third catheter, the one end that the culture tank was kept away from to the third catheter runs through the inside wall of outer case, incubator and detection case and extends to the inside of detection case, the inside of culture tank communicates with the inside of detection case through the third catheter, the inside of third catheter is provided with the motorised valve, the output setting of third catheter is in the top of detecting the ware.

Optionally, the one end that the filter was kept away from to the breather pipe runs through the lateral wall of outer case and incubator and extends to the inside of incubator, carbon dioxide sensor is installed in the inside left side of incubator, carbon dioxide sensor and external control terminal electric connection.

Optionally, the top fixedly connected with inlet pipe of interpolation liquid storage box, the one end that the interpolation liquid storage box was kept away from to the inlet pipe runs through the upper surface of detection case and extends to the outside of detection case, the inlet pipe communicates with the inside of interpolation liquid storage box, the bottom of interpolation liquid storage box is provided with a plurality of fourth catheter, the inside of fourth catheter is provided with the motorised valve, the right side of detection case is provided with the chamber door.

Compared with the prior art, the utility model has the beneficial effects that:

1. the device places case, first catheter, culture solution and places case, second catheter and motorised valve through the cell and comes leading-in incubator respectively with natural killer cell and culture solution in, avoids the operator to frequently open the incubator and operates, leads to outside microorganism to get into the incubator, influences the problem that the cell normally developed, controls the temperature of the inside water of chamber that adjusts temperature through temperature regulator, heating pipe and refrigeration pipe simultaneously to the inside temperature of control incubator adjusts to the temperature that natural killer cell suitably developed.

2. Clean carbon dioxide is generated through the filter and the air pump, the clean carbon dioxide drives the inside of the incubator through the vent pipe, the culture of natural killer cells is facilitated, the concentration of the carbon dioxide inside the incubator is detected through the carbon dioxide sensor, the humidity inside the incubator is ensured through the moisture machine, and the development of the natural killer cells is also facilitated.

3. The liquid is added to the inside interpolation liquid of liquid storage tank through the fourth catheter and is brought to the detection ware, takes place the reaction and detects with the natural killer cell that cultivates, controls the input of adding the liquid through a plurality of fourth catheters simultaneously, and the device function is various, has stronger practicality.

Drawings

FIG. 1 is a schematic perspective view of the present utility model;

FIG. 2 is a cross-sectional view of the present utility model;

reference numerals illustrate:

1. an outer case; 11. a first leg; 12. a transparent glass plate;

2. an incubator; 21. a temperature regulating cavity;

3. a cell placement box; 31. a first cover; 32. a first catheter;

4. a culture solution placing box; 41. a second cover; 42. a second catheter;

5. a culture tank; 51. a third catheter;

6. a temperature regulator; 61. heating pipes; 62. a refrigeration tube;

7. a filter; 71. an air pump; 72. a vent pipe; 73. a carbon dioxide sensor;

8. a moisture machine;

9. a detection box; 91. a second leg; 92. an additive liquid storage tank; 93. a feed pipe; 94. a fourth catheter; 95. a door;

10. the dish was tested.

Description of the embodiments

The following description is presented to enable one of ordinary skill in the art to make and use the utility model. The preferred embodiments in the following description are by way of example only and other obvious variations will occur to those skilled in the art.

Referring to FIGS. 1-2, a natural killer cell culturing and detecting apparatus comprises an outer case 1 and a detecting case 9, wherein first supporting legs 11 are fixedly connected to four corners of the bottom surface of the outer case 1, a transparent glass plate 12 is arranged on the upper surface of the outer case 1, the condition of inputting culture solution is conveniently observed through the transparent glass plate 12, an incubator 2 is arranged in the outer case 1, a temperature adjusting cavity 21 is formed between the outer side wall of the incubator 2 and the inner side wall of the outer case 1, a cell placing case 3 and a culture solution placing case 4 are fixedly arranged on the upper surface of the outer case 1 and on the left and right sides of the transparent glass plate 12 respectively, a first case cover 31 is arranged at the top of the cell placing case 3, a first liquid guide tube 32 is fixedly connected to the bottom of the cell placing case 3, one end of the first liquid guide tube 32, which is far away from the cell placing case 3, penetrates through the upper surface of the outer case 1 and extends into the interior of the incubator 2, the inside of the cell placement tank 3 is communicated with the inside of the incubator 2 through a first liquid guide tube 32, the top of the culture solution placement tank 4 is provided with a second tank cover 41, the bottom of the culture solution placement tank 4 is fixedly connected with a second liquid guide tube 42, one end of the second liquid guide tube 42, which is far away from the culture solution placement tank 4, penetrates through the upper surface of the outer tank 1 and extends to the inside of the incubator 2, the inside of the culture solution placement tank 4 is communicated with the inside of the incubator 2 through the second liquid guide tube 42, the inside of the first liquid guide tube 32 and the inside of the second liquid guide tube 42 are respectively provided with an electric valve, natural killer cells are placed in the cell placement tank 3, the electric valves are opened, the natural killer cells are guided into the incubator 2 through the first liquid guide tube 32, the culture solution is placed in the culture solution placement tank 4, the electric valves are opened, the culture solution flows into the incubator 2 through the second liquid guide tube 42, the culture tank 5 is arranged below the inside of the incubator 2, the culture tank 5 is arranged below the first liquid guide pipe 32 and the second liquid guide pipe 42, a third liquid guide pipe 51 is fixedly connected to the lower position of the right side of the culture tank 5, one end, far away from the culture tank 5, of the third liquid guide pipe 51 penetrates through the outer box 1, the culture tank 2 and the inner side wall of the detection box 9 and extends to the inside of the detection box 9, the inside of the culture tank 5 is communicated with the inside of the detection box 9 through the third liquid guide pipe 51, an electric valve is arranged in the inside of the third liquid guide pipe 51, natural killer cells and culture liquid respectively flow into the culture tank 5 in a potential mode, after culture, the cultured natural killer cells flow out through the third liquid guide pipe 51 and enter the detection box 9 in a homeopathic mode, and detection of the natural killer cells is started.

Further, as shown in FIG. 2, the bottom surface of the outer box 1 is fixedly provided with a temperature regulator 6, both sides of the top of the temperature regulator 6 are respectively provided with a heating pipe 61 and a refrigerating pipe 62, the working ends of the heating pipe 61 and the refrigerating pipe 62 penetrate through the bottom surface of the outer box 1 and extend into the temperature regulating cavity 21, the temperature of water in the temperature regulating cavity 21 is controlled through the temperature regulator 6, the heating pipe 61 and the refrigerating pipe 62, so that the temperature in the incubator 2 is controlled, the temperature is regulated to a temperature suitable for the development of natural killer cells, the left side of the outer box 1 is fixedly provided with a filter 7, the left side of the filter 7 is provided with an air pump 71, the output end of the filter 7 is fixedly connected with a vent pipe 72, one end that filter 7 was kept away from to breather pipe 72 runs through the lateral wall of outer case 1 and incubator 2 and extends to the inside of incubator 2, carbon dioxide sensor 73 is installed in the inside left side of incubator 2, carbon dioxide sensor 73 and outside control terminal electric connection, generate clean carbon dioxide through filter 7 and air pump 71, drive the inside of incubator 2 through breather pipe 72, be favorable to natural killer cell's cultivation, detect the inside carbon dioxide's of incubator 2 concentration through carbon dioxide sensor 73, the inside rear side fixed mounting of incubator 2 has moisture machine 8, moisture machine 8 and outside control terminal electric connection guarantee the inside humidity of incubator 2 through moisture machine 8, equally be favorable to natural killer cell's development.

In another possible embodiment, as shown in fig. 2, the detecting box 9 is disposed on the right side of the outer box 1, the second supporting leg 91 is fixedly connected to the two corner positions on the right side of the bottom surface of the detecting box 9, the adding liquid storage box 92 is disposed in the detecting box 9, the feeding pipe 93 is fixedly connected to the top of the adding liquid storage box 92, one end of the feeding pipe 93, far away from the adding liquid storage box 92, penetrates through the upper surface of the detecting box 9 and extends to the outside of the detecting box 9, the feeding pipe 93 is communicated with the inside of the adding liquid storage box 92, a plurality of fourth liquid guide pipes 94 are disposed at the bottom of the adding liquid storage box 92, an electric valve is disposed in the inside of the fourth liquid guide pipes 94, a box door 95 is disposed on the right side of the detecting box 9, the detecting box 10 is disposed below the inside of the detecting box 9, the output end of the third liquid guide pipe 51 is disposed above the detecting box 10, the adding liquid enters the adding liquid storage box 92 through the feeding pipe 93, the adding liquid in the adding liquid storage box 92 is carried into the detecting box 10 through the fourth liquid guide pipes 94, the adding liquid in the detecting box 92 reacts with natural killer cells and is detected, the detecting liquid is well, the detecting liquid is detected and the detecting box is taken out, and the detecting box 95 is more strongly and the detecting box is replaced by the fourth liquid guide pipe is more than the detecting box 9, and has the function of inputting the function of the detecting box is more than the detecting box 9.

The working principle of the utility model is as follows: firstly, natural killer cells are placed in a cell placement box 3, an electric valve is opened, the natural killer cells are led into the incubator 2 through a first liquid guide pipe 32, then a culture solution is placed in a culture solution placement box 4, the electric valve is opened, the culture solution flows into the incubator 2 through a second liquid guide pipe 42, the natural killer cells and the culture solution respectively flow into a culture tank 5 along the potential direction, the temperature of a heating pipe 61 and a refrigerating pipe 62 is controlled through a temperature regulator 6, the temperature of water in a temperature regulating cavity 21 is further controlled, the temperature in the incubator 2 is further controlled, the temperature is regulated to be suitable for the growth of the natural killer cells, then a filter 7 and an air pump 71 are opened to generate clean carbon dioxide, the air pump 71 is used for driving the inside of the incubator 2 to be beneficial to the culture of the natural killer cells, a moisture machine 8 is opened to ensure the humidity in the inside of the incubator 2, the growth of the natural killer cells is also beneficial to the growth of the natural killer cells, finally, an additive solution in the additive solution storage box 92 is introduced into an additive solution storage box 92 through a feed pipe 93, the additive solution in a fourth liquid guide pipe 94 is brought into a detection 10, the natural killer cells in the culture dish is reacted with the culture dish, and the natural killer cells are detected.

The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made therein without departing from the spirit and scope of the utility model, which is defined by the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims (8)

1. A natural killer cell culture and detection device, characterized in that: including outer case (1) and detection case (9), the inside of outer case (1) is provided with incubator (2), form between the inside wall of incubator (2) lateral wall and outer case (1) and temperature regulating chamber (21), the upper surface of outer case (1) and be located the left and right sides of transparent glass board (12) and fixed mounting respectively have cell to place case (3) and incubator (4), the inside below of incubator (2) is provided with incubator (5), the bottom surface fixed mounting of outer case (1) has temperature regulator (6), the top both sides of temperature regulator (6) are provided with heating pipe (61) and refrigeration pipe (62) respectively, the work end of heating pipe (61) and refrigeration pipe (62) all runs through the bottom surface of outer case (1) and extends to the inside of temperature regulating chamber (21), the left side fixed mounting of outer case (1) has filter (7), the left side of filter (7) is provided with air pump (71), the output fixed connection of filter (7) has temperature regulator (6), moisture control terminal (8) are installed at the inside of humidity control machine (8) and outside of humidity control machine (8), the two corner positions on the right side of the bottom surface of the detection box (9) are fixedly connected with second supporting legs (91), an additive liquid storage box (92) is arranged in the detection box (9), and a detection dish (10) is placed below the inner part of the detection box (9).

2. The natural killer cell culturing and detecting apparatus according to claim 1, wherein: the upper surface of outer case (1) is provided with transparent glass board (12), all fixedly connected with first stabilizer blade (11) in bottom surface four corners position department of outer case (1).

3. The natural killer cell culturing and detecting apparatus according to claim 1, wherein: the cell placement box is characterized in that a first box cover (31) is arranged at the top of the cell placement box (3), a first liquid guide pipe (32) is fixedly connected to the bottom of the cell placement box (3), one end of the first liquid guide pipe (32), far away from the cell placement box (3), penetrates through the upper surface of the outer box (1) and extends to the inside of the incubator (2), and the inside of the cell placement box (3) is communicated with the inside of the incubator (2) through the first liquid guide pipe (32).

4. The natural killer cell culturing and detecting apparatus according to claim 1, wherein: the top of incubator (4) is provided with second case lid (41), the bottom fixedly connected with second catheter (42) of incubator (4) is placed to the incubator, the one end that incubator (4) was placed to second catheter (42) is kept away from to the incubator runs through the upper surface of outer case (1) and extends to the inside of incubator (2), the inside of incubator (4) is placed through second catheter (42) and the inside intercommunication of incubator (2).

5. A natural killer cell culture and detection apparatus according to claim 3, wherein: the first catheter (32) and the second catheter (42) are provided with electrically operated valves inside.

6. The natural killer cell culturing and detecting apparatus according to claim 1, wherein: the culture tank (5) is arranged below the first liquid guide pipe (32) and the second liquid guide pipe (42), a third liquid guide pipe (51) is fixedly connected to the right side of the culture tank (5) at a position below, one end of the third liquid guide pipe (51) away from the culture tank (5) penetrates through the inner side wall of the outer box (1), the inner side wall of the culture box (2) and the inner side wall of the detection box (9) and extends to the inner side of the detection box (9), the inner side of the culture tank (5) is communicated with the inner side of the detection box (9) through the third liquid guide pipe (51), an electric valve is arranged in the inner side of the third liquid guide pipe (51), and the output end of the third liquid guide pipe (51) is arranged above the detection dish (10).

7. The natural killer cell culturing and detecting apparatus according to claim 1, wherein: one end of a breather pipe (72) far away from the filter (7) penetrates through the outer side walls of the outer box (1) and the incubator (2) and extends to the inside of the incubator (2), a carbon dioxide sensor (73) is arranged on the left side of the inside of the incubator (2), and the carbon dioxide sensor (73) is electrically connected with an external control terminal.

8. The natural killer cell culturing and detecting apparatus according to claim 1, wherein: the top fixedly connected with inlet pipe (93) of interpolation liquid storage case (92), the one end that interpolation liquid storage case (92) was kept away from to inlet pipe (93) runs through the upper surface of detection case (9) and extends to the outside of detection case (9), inlet pipe (93) communicate with the inside of interpolation liquid storage case (92), the bottom of interpolation liquid storage case (92) is provided with fourth catheter (94), the inside of fourth catheter (94) is provided with the motorised valve, the right side of detection case (9) is provided with chamber door (95).