CN107951829A - A kind of oxidation resistant skin care item of the extract of stem cell containing azalea - Google Patents

A kind of oxidation resistant skin care item of the extract of stem cell containing azalea Download PDF

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Publication number
CN107951829A
CN107951829A CN201711315678.9A CN201711315678A CN107951829A CN 107951829 A CN107951829 A CN 107951829A CN 201711315678 A CN201711315678 A CN 201711315678A CN 107951829 A CN107951829 A CN 107951829A
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China
Prior art keywords
skin care
extract
parts
stem cell
azalea
Prior art date
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Application number
CN201711315678.9A
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Chinese (zh)
Inventor
葛啸虎
陈海佳
王飞
王一飞
王小燕
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Guangzhou Saliai StemCell Science and Technology Co Ltd
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Guangzhou Saliai StemCell Science and Technology Co Ltd
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Priority to CN201711315678.9A priority Critical patent/CN107951829A/en
Publication of CN107951829A publication Critical patent/CN107951829A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)

Abstract

The invention discloses a kind of oxidation resistant skin care item of the extract of stem cell containing azalea, belong to technical field of skin care, skin care compositions includes the component of following mass fraction：3~8 parts of azalea stem cell extract, 11~17 parts of bodhi flower extract and blue or green 9~14 parts of bur extract；The mass percentage of skin care compositions is 3.6% in skin care item.Not but not damage skin, and oxidation resistant effect is not good for the skin care compositions and skin care item of the present invention.

Description

A kind of oxidation resistant skin care item of the extract of stem cell containing azalea
Technical field
The invention belongs to technical field of skin care, more particularly to a kind of oxidation resistant shield of stem cell containing azalea extract Skin product.
Background technology
With the improvement of living standard, more and more people begin to focus on beautifying face and moistering lotion.In the market skin care item species is various, has Anti-aging skin care item, remove wrinkle skin care item, and moist face nourishing skin care products etc..However, add in existing anti-oxidant skin care item The synthesis chemical reagent added is excessive, easily damages skin；Secondly, oxidation resistant effect is poor.
Therefore, it is necessary to prepare new anti-oxidant skin care item, its not but not damage skin, and oxidation resistant work( Imitate.
The content of the invention
Present invention aims to overcome that the shortcomings of the prior art, and a kind of extract of stem cell containing azalea is provided Oxidation resistant skin care item, not but not damage skin, and oxidation resistant effect is not good for the skin care item.
To achieve the above object, the technical solution taken of the present invention is：A kind of oxidation resistant skin care compositions, it includes with The component of lower mass fraction：3~8 parts of azalea stem cell extract, 11~17 parts of bodhi flower extract and blue or green bur extract 9 ~14 parts.
The preparation method of azalea stem cell extract is：
1) by the tender leaf of azalea after disinfecting, chopping is seeded on MS culture mediums, dark culturing at 26 DEG C；Two The outer paraphyte of forming layer had significant proliferation is taken out after week, stem cell is separated and is transferred in subculture medium and cultivate, every two weeks Subculture once, obtains azalea stem cell；
2) azalea stem cell is cracked, is freeze-dried afterwards, obtains azalea stem cell extract.
Azalea stem cell extract contains a variety of active ingredients, can carry out moisturizing to skin and improve the bullet of skin Property, and there is whitening spot-removing.Bodhi flower extract is rich in vitamin C, and bodhi flower extract can remove free radical, tool There is oxidation resistant effect.Blue or green bur extract is rich in multivitamin, has effects that oxidation resistant.
The skin care compositions of the present invention includes azalea stem cell extract, bodhi flower extract and blue or green bur extract, Azalea stem cell extract, bodhi flower extract and blue or green bur extract can have anti-oxidant in the range of certain proportion The effect of：Remove free radical；And the skin care compositions is mostly natural materials, to skin zero damage.
As the improvement of above-mentioned technical proposal, skin care compositions includes the component of following mass fraction：Azalea stem cell 4~7 parts of extract, 14~16 parts of bodhi flower extract and blue or green 12~14 parts of bur extract.
Further as above-mentioned technical proposal is improved, the quality of the bodhi flower extract and the blue or green bur extract Than for 1~1.2.When bodhi flower extract in skin care compositions and the mass ratio of blue or green bur extract are 1~1.2, bodhi Extract can cooperate with the oxidation resistant effect for strengthening skin care compositions with blue or green bur extract.
Further as above-mentioned technical proposal is improved, the matter of the bodhi flower extract and the blue or green bur extract Amount is than being 1.
Further as above-mentioned technical proposal is improved, the matter of the bodhi flower extract and the blue or green bur extract Amount is than being 1.1.
Further as above-mentioned technical proposal is improved, and skin care compositions is made of the component of following mass fraction：Du 5 parts of cuckoo flower stem cell extract, 14 parts of bodhi flower extract and blue or green 14 parts of bur extract.
Further as above-mentioned technical proposal is improved, and skin care compositions is made of the component of following mass fraction：Du 4.6 parts of cuckoo flower stem cell extract, 14.3 parts of bodhi flower extract and 13 parts of the extract that please cut to the bone.
In addition, the application the present invention also provides the skin care compositions in skin care item.
In addition, the present invention also provides a kind of skin care item for including the skin care compositions.
As the improvement of above-mentioned technical proposal, the skin care item are made of the component of lower mass percentage：Skincare composition Thing 3.6%, moisturizer 12.8% and emulsifying agent 19.6%, surplus are deionized water.
The beneficial effects of the present invention are：The present invention provides a kind of oxidation resistant skin care of the extract of stem cell containing azalea Product, azalea stem cell extract, bodhi flower extract and blue or green bur extract in skin care compositions and skin care item of the invention Quality in the range of certain proportion, can efficiently to skin carry out it is anti-oxidant；It is and more in the skin care compositions and skin care item For natural materials, to skin zero damage.
Embodiment
For the object, technical solutions and advantages of the present invention are better described, below in conjunction with specific embodiment and subordinate list pair The present invention is described further.
Embodiment 1
A kind of oxidation resistant skin care compositions, it is made of the component of following mass fraction：Azalea stem cell extract 3 Part, 11 parts of bodhi flower extract and blue or green 14 parts of bur extract.
The preparation method of azalea stem cell extract is：
1) by the tender leaf of azalea after disinfecting, chopping is seeded on MS culture mediums, dark culturing at 26 DEG C；Two The outer paraphyte of forming layer had significant proliferation is taken out after week, stem cell is separated and is transferred in subculture medium and cultivate, every two weeks Subculture once, obtains azalea stem cell；
2) azalea stem cell is cracked, is freeze-dried afterwards, obtains azalea stem cell extract.
The present embodiment also provides a kind of skin care item, it is made of the component of following mass percentage：Skin care compositions 3.6%th, moisturizer (butantriol and glycerine) 12.8% and emulsifying agent (sorbierite olive grease) 19.6%, surplus is deionization Water.
Embodiment 2
A kind of oxidation resistant skin care compositions, it is made of the component of following mass fraction：Azalea stem cell extract 6.7 parts, 17 parts of bodhi flower extract and blue or green 9 parts of bur extract.
The present embodiment also provides a kind of skin care item, it is made of the component of following mass percentage：Skin care compositions 3.6%th, moisturizer (butantriol and glycerine) 12.8% and emulsifying agent (sorbierite olive grease) 19.6%, surplus is deionization Water.
Embodiment 3
A kind of oxidation resistant skin care compositions, it is made of the component of following mass fraction：Azalea stem cell extract 8 Part, 12 parts of bodhi flower extract and blue or green 13.1 parts of bur extract.
The present embodiment also provides a kind of skin care item, it is made of the component of following mass percentage：Skin care compositions 3.6%th, moisturizer (butantriol and glycerine) 12.8% and emulsifying agent (sorbierite olive grease) 19.6%, surplus is deionization Water.
Embodiment 4
A kind of oxidation resistant skin care compositions, it is made of the component of following mass fraction：Azalea stem cell extract 5 Part, 14 parts of bodhi flower extract and blue or green 14 parts of bur extract.
The present embodiment also provides a kind of skin care item, it is made of the component of following mass percentage：Skin care compositions 3.6%th, moisturizer (butantriol and glycerine) 12.8% and emulsifying agent (sorbierite olive grease) 19.6%, surplus is deionization Water.
Embodiment 5
A kind of oxidation resistant skin care compositions, it is made of the component of following mass fraction：Azalea stem cell extract 4.6 parts, 14.3 parts of bodhi flower extract and blue or green 13 parts of bur extract.
The present embodiment also provides a kind of skin care item, it is made of the component of following mass percentage：Skin care compositions 3.6%th, moisturizer (butantriol and glycerine) 12.8% and emulsifying agent (sorbierite olive grease) 19.6%, surplus is deionization Water.
Embodiment 6
A kind of oxidation resistant skin care compositions, it is made of the component of following mass fraction：Azalea stem cell extract 6 Part, 14.4 parts of bodhi flower extract and blue or green 12 parts of bur extract.
The present embodiment also provides a kind of skin care item, it is made of the component of following mass percentage：Skin care compositions 3.6%th, moisturizer (butantriol and glycerine) 12.8% and emulsifying agent (sorbierite olive grease) 19.6%, surplus is deionization Water.
Comparative example 1
A kind of oxidation resistant skin care compositions, it is made of the component of following mass fraction：Azalea stem cell extract 15 parts, 8 parts of bodhi flower extract and blue or green 12 parts of bur extract.
The present embodiment also provides a kind of skin care item, it is made of the component of following mass percentage：Skin care compositions 3.6%th, moisturizer (butantriol and glycerine) 12.8% and emulsifying agent (sorbierite olive grease) 19.6%, surplus is deionization Water.
Comparative example 2
A kind of oxidation resistant skin care compositions, it is made of the component of following mass fraction：Azalea stem cell extract 5.7 parts, 6.3 parts of bodhi flower extract and blue or green 18.1 parts of bur extract.
The present embodiment also provides a kind of skin care item, it is made of the component of following mass percentage：Skin care compositions 3.6%th, moisturizer (butantriol and glycerine) 12.8% and emulsifying agent (sorbierite olive grease) 19.6%, surplus is deionization Water.
Effect example 1
1) antioxidation activity is represented with scavenging ability of DPPH free radical size：Skin care item 2.0g is taken, adds 2.0ml1.52 ×10-4The DPPH ethanol solutions of mmol/l, and fully mix, after room temperature lucifuge stands 30min, using solvent as blank pair According to survey absorbance at 517nm；Every group is done 3 repetitions, is averaged.
This experiment is randomly divided into 5 groups：The skin care item of embodiment 1 are used in A11 groups, B11 groups use the skin care of embodiment 2 Product, C11 groups use the skin care item of embodiment 3, and D11 groups use the skin care item of comparative example 1, and E11 groups use the skin care of comparative example 2 Product, measurement result are as shown in table 1.
Table 1
Group A11 groups B11 groups C11 groups D11 groups E11 groups
Clearance rate/% of DPPH free radicals 30.4 31.1 31.4 20.3 21.7
2) antioxidation activity is represented with removing hydroxyl radical free radical capacity of water：By 1.5ml 1.8mol/l salicylic acid solutions With 2.0ml 1.8mol/l FeSO4After solution mixes in test tube, 2.0g skin care item are separately added into, then add 0.1ml 0.03%H2O2, shake up and keep the temperature 30min after 37 DEG C, measure absorbance at 510nm, calculate the clearance rate of hydroxyl radical free radical；Often Group does 3 repetitions, is averaged.
This experiment is randomly divided into 5 groups：The skin care item of embodiment 1 are used in A12 groups, B12 groups use the skin care of embodiment 2 Product, C12 groups use the skin care item of embodiment 3, and D12 groups use the skin care item of embodiment 4, and E12 groups use the skin care of embodiment 5 Product, measurement result are as shown in table 2.
Table 2
Group A12 groups B12 groups C12 groups D12 groups E12 groups
Clearance rate/% of hydroxyl radical free radical 29.3 30.0 28.7 15.7 10.2
As shown in table 1, the clearance rate of the DPPH free radicals of A11, B11 and C11 group is more than the DPPH freedom of D11 and E11 groups The clearance rate of base；As shown in table 2, the clearance rate of the hydroxyl radical free radical of A12, B12 and C12 group is more than the hydroxyl of D12 and E12 groups certainly By the clearance rate of base；It can be seen from the above that when azalea stem cell extract in skin care compositions mass fraction be 3~8 parts, bodhi The mass fraction of flower extract is 11~17 parts, when the mass fraction of blue or green bur extract is 9~14 parts, skin care compositions and shield The anti-oxidation efficacy of skin product is preferable.
Effect example 2
1) antioxidation activity is represented with scavenging ability of DPPH free radical size, with reference to effect example 1, random point of this experiment Into 6 groups：A21 groups use the skin care item of embodiment 1, and B21 groups use the skin care item of embodiment 2, and C21 groups use the shield of embodiment 3 Skin product, D21 groups use the skin care item of embodiment 4, and E21 groups use the skin care item of embodiment 5, and F21 groups use the skin care of embodiment 6 Product, measurement result are as shown in table 3.
Table 3
Group A21 groups B21 groups C21 groups D21 groups E21 groups F21 groups
Clearance rate/% of DPPH free radicals 30.4 31.1 31.4 45.6 46 41.7
2) antioxidation activity is represented with removing hydroxyl radical free radical capacity of water, with reference to effect example 1, random point of this experiment Into 6 groups：A22 groups use the skin care item of embodiment 1, and B22 groups use the skin care item of embodiment 2, and C22 groups use the shield of embodiment 3 Skin product, D22 groups use the skin care item of embodiment 4, and E22 groups use the skin care item of embodiment 5, and F22 groups use the skin care of embodiment 6 Product, measurement result are as shown in table 4.
Table 4
Group A22 groups B22 groups C22 groups D22 groups E22 groups F22 groups
Clearance rate/% of hydroxyl radical free radical 29.3 30.0 28.7 40 41.2 37.3
As shown in table 3, the clearance rate of the DPPH free radicals of the application D21, E21 and F21 groups is more than A21, B21 and C21 group DPPH free radicals clearance rate；As shown in table 4, the clearance rate of the hydroxyl radical free radical of D22, E22 and F22 group is more than A22, B22 With the clearance rate of the hydroxyl radical free radical of C22 groups；It can be seen from the above that when the mass parts of azalea stem cell extract in skin care compositions Number is 3~8 parts, and the mass fraction of bodhi flower extract is 11~17 parts, and the mass fraction of blue or green bur extract is 9~14 parts, And the mass ratio of bodhi flower extract and prinsepia utilis royle oil, when being 1~1.2, bodhi flower extract can be cooperateed with blue or green bur extract to be added The anti-oxidation efficacy of oxidation resistant effect of strong skin care compositions, skin care compositions and skin care item is more preferable.
Finally, it should be noted that above example to illustrate technical scheme rather than to the present invention protect The limitation of scope, although being explained in detail with reference to preferred embodiment to the present invention, those of ordinary skill in the art should manage Solution, can modify to technical scheme or replace on an equal basis, without departing from technical solution of the present invention essence and Scope.

Claims (10)

1. a kind of oxidation resistant skin care compositions, it is characterised in that include the component of following mass fraction：Azalea stem cell carries Take 3~8 parts of thing, 11~17 parts of bodhi flower extract and blue or green 9~14 parts of bur extract.
2. skin care compositions as claimed in claim 1, it is characterised in that include the component of following mass fraction：Azalea does 4~7 parts of cell extract, 14~16 parts of bodhi flower extract and blue or green 12~14 parts of bur extract.
3. skin care compositions as claimed in claim 1 or 2, it is characterised in that the bodhi flower extract and the blue or green bur The mass ratio of extract is 1~1.2.
4. skin care compositions as claimed in claim 3, it is characterised in that the bodhi flower extract is extracted with the blue or green bur The mass ratio of thing is 1.
5. skin care compositions as claimed in claim 3, it is characterised in that the bodhi flower extract is extracted with the blue or green bur The mass ratio of thing is 1.1.
6. skin care compositions as claimed in claim 4, it is characterised in that be made of the component of following mass fraction：Azalea 5 parts of stem cell extract, 14 parts of bodhi flower extract and blue or green 14 parts of bur extract.
7. skin care compositions as claimed in claim 5, it is characterised in that be made of the component of following mass fraction：Azalea 4.6 parts of stem cell extract, 14.3 parts of bodhi flower extract and blue or green 13 parts of bur extract.
8. such as application of the claim 1~7 any one of them skin care compositions in skin care item.
9. a kind of power such as that includes requires the skin care item of 1~7 any one of them skin care compositions.
10. skin care item as claimed in claim 9, it is characterised in that be made of the component of following mass percentage：Skin care group Compound 3.6%, moisturizer 12.8% and emulsifying agent 19.6%, surplus are deionized water.
CN201711315678.9A 2017-12-07 2017-12-07 A kind of oxidation resistant skin care item of the extract of stem cell containing azalea Withdrawn CN107951829A (en)

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Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101690704A (en) * 2009-08-21 2010-04-07 珈侬生化科技(中国)有限公司 Rhododendron active extract preparation and whitening anti-aging active cosmetic
CN105748343A (en) * 2016-02-25 2016-07-13 广州市科玮生物技术有限公司 Natural plant extract composition and application thereof in cosmetics

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101690704A (en) * 2009-08-21 2010-04-07 珈侬生化科技(中国)有限公司 Rhododendron active extract preparation and whitening anti-aging active cosmetic
CN105748343A (en) * 2016-02-25 2016-07-13 广州市科玮生物技术有限公司 Natural plant extract composition and application thereof in cosmetics

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
王建新主编: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 *

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Application publication date: 20180424