CN107951820A - A kind of stem cell skin care composition and preparation method thereof - Google Patents

A kind of stem cell skin care composition and preparation method thereof Download PDF

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Publication number
CN107951820A
CN107951820A CN201711252446.3A CN201711252446A CN107951820A CN 107951820 A CN107951820 A CN 107951820A CN 201711252446 A CN201711252446 A CN 201711252446A CN 107951820 A CN107951820 A CN 107951820A
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stem cell
component
care composition
parts
skin care
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熊华强
胡小东
宋彩霞
黄启程
王仁杰
邱阳
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Chongqing Golden Times Biotechnology Co Ltd
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Chongqing Golden Times Biotechnology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/345—Alcohols containing more than one hydroxy group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/678—Tocopherol, i.e. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/86—Products or compounds obtained by genetic engineering

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Cosmetics (AREA)

Abstract

The invention discloses a kind of stem cell skin care composition, including component A, B component, component C, hydrophilic gelling agent and water, its raw material are as follows by weight：Component A includes 20 35 parts of at least one cyclic diester of 20 50 parts of stem cell extract, 3 11 parts of seralbumin, alpha-hydroxy acid；B component includes 26 parts of mannitol, 12 16 parts of SOD enzyme liquids, 59 parts of vitamin E, 46 parts of ammonium citrate；Component C is the 1 1.5% of stem cell skin care composition quality, includes moisturizer, skin conditioner, preservative and dispersant；Hydrophilic gelling agent is the 1 5% of stem cell skin care composition quality；Water is the 1 1.3% of stem cell skin care composition quality, the all multiple-factor synergistic effects of the present invention, and can effectively realize and these factors are passed through into skin, from the metabolism of deep layer enhancing skin, delay skin aging, achievees the purpose that whitening, prevention or improves skin wrinkle and wrinkle, go acne, is hypo-allergenic.

Description

A kind of stem cell skin care composition and preparation method thereof
Technical field
The present invention relates to stem cell skin care composition technical field, more particularly to a kind of stem cell skin care composition and its system Preparation Method.
Background technology
The currently available many personal care products of consumer relate generally to improve, keep outside the health and physics of skin See.Skin care compositions includes emollient and siloxanes to provide flexibility, smoothness and supplement iuntercellular lipoid to skin.Bag Skin care compositions containing ultra-violet protecting agent prevents from skin texture changing due to making cuticula excessively peel off exposed to ultraviolet light.When When skin care compositions includes the oily components of increment, usual way is the surfactant comprising increment to emulsify or dispersed oil Property component.However, the surfactant of increment equally provides greasy feeling or sticky feeling.Consumer for preferring fresh feel For, above-mentioned greasy feeling or sticky feeling are undesirable.Meanwhile the metabolic function of its enhancing skin is weaker, delays skin Skin aging unobvious.
The content of the invention
The purpose of the present invention is to solve shortcoming existing in the prior art, and a kind of stem cell skincare composition proposed Thing and preparation method thereof.
To achieve these goals, present invention employs following technical solution：
A kind of stem cell skin care composition, including component A, B component, component C, hydrophilic gelling agent and water, its raw material is by weight It is as follows to measure part：Component A includes 20-50 parts of stem cell extract, 3-11 parts of seralbumin, at least one ring of alpha-hydroxy acid 20-35 parts of shape diester；B component includes 2-6 parts of mannitol, 12-16 parts of SOD enzyme liquids, 5-9 parts of vitamin E, ammonium citrate 4-6 Part；Component C is the 1-1.5% of stem cell skin care composition quality, includes moisturizer, skin conditioner, preservative and dispersant；Parent Water gelling agent is the 1-5% of stem cell skin care composition quality；Water is the 1-1.3% of stem cell skin care composition quality.
Preferably, the stem cell extract is the stem cell media and the jelly of culture medium stem cell after culture stem cell Melt lysate.
Preferably, the moisturizer is silk peptide, and the skin conditioner is lanolin, and the preservative is tea polyphenols, described point Powder is soybean protein surfactant.
Preferably, the hydrophilic gelling agent is Voncoat R 3310 and the mixture of acrylate copolymer.
A kind of preparation method of stem cell skin care composition, includes the following steps：
S1：Prepared by stem cell extract includes the expression of stem cell origin, the stem cell factor that plasmid electricity referral is led expresses, is slow Virus-mediated stem cell factor expression or the expression of adenovirus mediated stem cell factor, wherein, plasmid electricity referral is led dry When prepared by cytokine-expressing, the stem cell factor expression of lentivirus mediated or the expression of adenovirus mediated stem cell factor, After stem cell factor and permeable membrane peptide gene series are constructed new fusion, then carry out subsequent step, cell factor with Permeable membrane peptide gene series can be inquired about by amino acid series in password sublist.
S2：At least one cyclic diester of seralbumin and alpha-hydroxy acid is put into stem cell extract and is stirred, Mixing time 3-5min, it is spare to obtain component A；
S3：Choose fresh tree peony leaf, after the impurity for removing surface, be cut into small pieces, add ultrasound in phosphate buffer Ripple processing, stirring obtain uniform solution, take supernatant to obtain thick enzyme filtrate after centrifugation；
S4：Thick enzyme filtrate prepared by step S3 is heated to 30-35 DEG C, adds mannitol, 20-30min is reacted in stirring, fast Quickly cooling but after, after ultrafiltration membrance filter, the SOD enzyme liquids modified；
S4：After the SOD enzyme liquid high-temperature sterilizations of the step S4 modifications prepared, add vitamin E and ammonium citrate stirring is equal It is even, obtain B component；
S5：By component A and B component, mixing in water, is stirred in mixing, after stirring 3-6min, added hydrophilic jointly Gelling agent, continues to stir, and is then added to wherein the moisturizer of component C, skin conditioner, preservative and dispersant again, stirs 10- After 15min, stem cell skin care composition is obtained.
A kind of stem cell skin care composition provided by the invention and preparation method thereof, all multiple-factor synergistic effects of the present invention, And can effectively realize and these factors are passed through into skin, from the metabolism of deep layer enhancing skin, delay skin aging, reaches beautiful In vain, prevent or improve skin wrinkle and wrinkle, go acne, hypo-allergenic purpose.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, below in conjunction with specific embodiment, to this Invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, not For limiting the present invention.
Embodiment 1
A kind of stem cell skin care composition, including component A, B component, component C, hydrophilic gelling agent and water, its raw material is by weight It is as follows to measure part：Component A includes 20 parts of stem cell extract, 3 parts of seralbumin, at least one cyclic diester of alpha-hydroxy acid 20 parts, stem cell extract is the stem cell media and the freeze-thaw lysate of culture medium stem cell after culture stem cell；B component Including 6 parts of mannitol, 16 parts of SOD enzyme liquids, 9 parts of vitamin E, 4 parts of ammonium citrate；Component C is stem cell skin care composition quality 1%, include moisturizer, skin conditioner, preservative and dispersant；Hydrophilic gelling agent is stem cell skin care composition quality 5%；Water is the 1% of stem cell skin care composition quality.
The moisturizer is silk peptide, and the skin conditioner is lanolin, and the preservative is tea polyphenols, and the dispersant is big Legumin surfactant.The hydrophilic gelling agent is Voncoat R 3310 and the mixture of acrylate copolymer.
A kind of preparation method of stem cell skin care composition, includes the following steps：
S1：Prepared by stem cell extract includes the expression of stem cell origin, the stem cell factor that plasmid electricity referral is led expresses, is slow Virus-mediated stem cell factor expression or the expression of adenovirus mediated stem cell factor, wherein, plasmid electricity referral is led dry When prepared by cytokine-expressing, the stem cell factor expression of lentivirus mediated or the expression of adenovirus mediated stem cell factor, After stem cell factor and permeable membrane peptide gene series are constructed new fusion.
S2：At least one cyclic diester of seralbumin and alpha-hydroxy acid is put into stem cell extract and is stirred, Mixing time 3min, it is spare to obtain component A；
S3：Choose fresh tree peony leaf, after the impurity for removing surface, be cut into small pieces, add ultrasound in phosphate buffer Ripple processing, stirring obtain uniform solution, take supernatant to obtain thick enzyme filtrate after centrifugation；
S4：Thick enzyme filtrate prepared by step S3 is heated to 30 DEG C, adds mannitol, stirring reaction 20min, rapid cooling Afterwards, after ultrafiltration membrance filter, the SOD enzyme liquids modified；
S4：After the SOD enzyme liquid high-temperature sterilizations of the step S4 modifications prepared, add vitamin E and ammonium citrate stirring is equal It is even, obtain B component；
S5：By component A and B component, mixing in water, stirs in mixing, after stirring 3min, adds hydrophilic gel jointly Solidifying agent, continues to stir, and is then added to wherein the moisturizer of component C, skin conditioner, preservative and dispersant again, stirs 10min Afterwards, stem cell skin care composition is obtained.
Embodiment 2
A kind of stem cell skin care composition, including component A, B component, component C, hydrophilic gelling agent and water, its raw material is by weight It is as follows to measure part：Component A includes 50 parts of stem cell extract, 3 parts of seralbumin, at least one cyclic diester of alpha-hydroxy acid 35 parts；B component includes 2 parts of mannitol, 16 parts of SOD enzyme liquids, 5 parts of vitamin E, 6 parts of ammonium citrate；Component C is stem cell skin care The 1.5% of composition quality, includes moisturizer, skin conditioner, preservative and dispersant；Hydrophilic gelling agent is stem cell skin care group The 5% of compound quality；Water is the 1% of stem cell skin care composition quality.
The stem cell extract is the stem cell media and the freezing-thawing and cracking of culture medium stem cell after culture stem cell Thing.The moisturizer is silk peptide, and the skin conditioner is lanolin, and the preservative is tea polyphenols, and the dispersant is soybean egg White surfactant.The hydrophilic gelling agent is Voncoat R 3310 and the mixture of acrylate copolymer.
A kind of preparation method of stem cell skin care composition, includes the following steps：
S1：Prepared by stem cell extract includes the expression of stem cell origin, the stem cell factor that plasmid electricity referral is led expresses, is slow Virus-mediated stem cell factor expression or the expression of adenovirus mediated stem cell factor, wherein, plasmid electricity referral is led dry When prepared by cytokine-expressing, the stem cell factor expression of lentivirus mediated or the expression of adenovirus mediated stem cell factor, After stem cell factor and permeable membrane peptide gene series are constructed new fusion, then carry out subsequent step, cell factor with Permeable membrane peptide gene series can be inquired about by amino acid series in password sublist.
S2：Choose fresh tree peony leaf, after the impurity for removing surface, be cut into small pieces, add ultrasound in phosphate buffer Ripple processing, stirring obtain uniform solution, take supernatant to obtain thick enzyme filtrate after centrifugation；
S3：Thick enzyme filtrate prepared by step S2 is heated to 35 DEG C, adds mannitol, stirring reaction 30min, rapid cooling Afterwards, after ultrafiltration membrance filter, the SOD enzyme liquids modified；
S4：After the SOD enzyme liquid high-temperature sterilizations of the step S3 modifications prepared, add vitamin E and ammonium citrate stirring is equal It is even, obtain B component；
S5：At least one cyclic diester of seralbumin and alpha-hydroxy acid is put into stem cell extract and is stirred, Mixing time 5min, it is spare to obtain component A；
S6：By component A and B component, mixing in water, stirs in mixing, after stirring 3min, adds hydrophilic gel jointly Solidifying agent, continues to stir, and is then added to wherein the moisturizer of component C, skin conditioner, preservative and dispersant again, stirs 15min Afterwards, stem cell skin care composition is obtained.
Embodiment 3
A kind of stem cell skin care composition, including component A, B component, component C, hydrophilic gelling agent and water, its raw material is by weight It is as follows to measure part：Component A includes 50 parts of stem cell extract, 3 parts of seralbumin, at least one cyclic diester of alpha-hydroxy acid 20 parts；B component includes 6 parts of mannitol, 16 parts of SOD enzyme liquids, 9 parts of vitamin E, 6 parts of ammonium citrate；Component C is stem cell skin care The 1.5% of composition quality, includes moisturizer, skin conditioner, preservative and dispersant；Hydrophilic gelling agent is stem cell skin care group The 5% of compound quality；Water is the 1.3% of stem cell skin care composition quality.
The stem cell extract is the stem cell media and the freezing-thawing and cracking of culture medium stem cell after culture stem cell Thing.The moisturizer is silk peptide, and the skin conditioner is lanolin, and the preservative is tea polyphenols, and the dispersant is soybean egg White surfactant.The hydrophilic gelling agent is Voncoat R 3310 and the mixture of acrylate copolymer.
A kind of preparation method of stem cell skin care composition, includes the following steps：
S1：Choose fresh tree peony leaf, after the impurity for removing surface, be cut into small pieces, add ultrasound in phosphate buffer Ripple processing, stirring obtain uniform solution, take supernatant to obtain thick enzyme filtrate after centrifugation；
S2：Thick enzyme filtrate prepared by step S1 is heated to 35 DEG C, adds mannitol, stirring reaction 30min, rapid cooling Afterwards, after ultrafiltration membrance filter, the SOD enzyme liquids modified；
S3：After the SOD enzyme liquid high-temperature sterilizations of the step S2 modifications prepared, add vitamin E and ammonium citrate stirring is equal It is even, obtain B component；
S4：Prepared by stem cell extract includes the expression of stem cell origin, the stem cell factor that plasmid electricity referral is led expresses, is slow Virus-mediated stem cell factor expression or the expression of adenovirus mediated stem cell factor, wherein, plasmid electricity referral is led dry When prepared by cytokine-expressing, the stem cell factor expression of lentivirus mediated or the expression of adenovirus mediated stem cell factor, After stem cell factor and permeable membrane peptide gene series are constructed new fusion.
S5：At least one cyclic diester of seralbumin and alpha-hydroxy acid is put into stem cell extract and is stirred, Mixing time 5min, it is spare to obtain component A；
S6：By component A and B component, mixing in water, stirs in mixing, after stirring 6min, adds hydrophilic gel jointly Solidifying agent, continues to stir, and is then added to wherein the moisturizer of component C, skin conditioner, preservative and dispersant again, stirs 15min Afterwards, stem cell skin care composition is obtained.
A kind of stem cell skin care composition provided by the invention and preparation method thereof, all multiple-factor synergistic effects of the present invention, And can effectively realize and these factors are passed through into skin, from the metabolism of deep layer enhancing skin, delay skin aging, reaches beautiful In vain, prevent or improve skin wrinkle and wrinkle, go acne, hypo-allergenic purpose.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto, Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims (5)

1. a kind of stem cell skin care composition, including component A, B component, component C, hydrophilic gelling agent and water, it is characterised in that：Its Raw material is as follows by weight：Component A includes 20-50 parts of stem cell extract, 3-11 parts of seralbumin, alpha-hydroxy acid extremely It is a kind of cyclic diester 20-35 parts few；B component includes 2-6 parts of mannitol, 12-16 parts of SOD enzyme liquids, 5-9 parts of vitamin E, lemon Sour ammonium 4-6 parts；Component C is the 1-1.5% of stem cell skin care composition quality, includes moisturizer, skin conditioner, preservative and divides Powder；Hydrophilic gelling agent is the 1-5% of stem cell skin care composition quality；Water is the 1- of stem cell skin care composition quality 1.3%.
A kind of 2. stem cell skin care composition according to claim 1, it is characterised in that：The stem cell extract is training Support the freeze-thaw lysate of stem cell media and culture medium stem cell after stem cell.
A kind of 3. stem cell skin care composition according to claim 1, it is characterised in that：The moisturizer is silk peptide, institute It is lanolin to state skin conditioner, and the preservative is tea polyphenols, and the dispersant is soybean protein surfactant.
A kind of 4. stem cell skin care composition according to claim 1, it is characterised in that：The hydrophilic gelling agent is propylene The mixture of acid ester homopolymer and acrylate copolymer.
A kind of 5. preparation method of the stem cell skin care composition described in claim 1, it is characterised in that：Include the following steps：
S1：Prepared by stem cell extract includes the expression of stem cell origin, the stem cell factor that plasmid electricity referral is led is expressed, slow virus The stem cell factor expression or the expression of adenovirus mediated stem cell factor of mediation, wherein, the stem cell that plasmid electricity referral is led , will be dry when prepared by factor expression, the stem cell factor expression of lentivirus mediated or the expression of adenovirus mediated stem cell factor After cell factor constructs new fusion with permeable membrane peptide gene series, then carry out subsequent step, cell factor and permeable membrane Peptide gene series can be inquired about by amino acid series in password sublist.
S2：At least one cyclic diester of seralbumin and alpha-hydroxy acid is put into stem cell extract and is stirred, is stirred Time 3-5min, it is spare to obtain component A；
S3：Choose fresh tree peony leaf, after the impurity for removing surface, be cut into small pieces, add in phosphate buffer at ultrasonic wave Reason, stirring obtain uniform solution, take supernatant to obtain thick enzyme filtrate after centrifugation；
S4：Thick enzyme filtrate prepared by step S3 is heated to 30-35 DEG C, adds mannitol, 20-30min is reacted in stirring, rapid cold But after, after ultrafiltration membrance filter, the SOD enzyme liquids modified；
S4：After the SOD enzyme liquid high-temperature sterilizations of the step S4 modifications prepared, add vitamin E and ammonium citrate stirs evenly, obtain To B component；
S5：By component A and B component, mixing in water, stirs in mixing, after stirring 3-6min, adds hydrophilic gelling jointly Agent, continues to stir, and is then added to wherein the moisturizer of component C, skin conditioner, preservative and dispersant again, stirs 10- After 15min, stem cell skin care composition is obtained.
CN201711252446.3A 2017-12-01 2017-12-01 A kind of stem cell skin care composition and preparation method thereof Pending CN107951820A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106983713A (en) * 2017-03-31 2017-07-28 杭州哈佛赛尔干细胞技术有限公司 A kind of stem cell skin care composition and preparation method thereof and application method
CN110368357A (en) * 2019-08-13 2019-10-25 江苏安泰生物技术有限公司 A kind of skin nursing Essence and preparation method thereof
CN110522703A (en) * 2019-06-12 2019-12-03 青岛华澳现代医疗美容有限公司 The skin care compositions and preparation method with skin selfreparing anti-aging for micropin
CN115444806A (en) * 2021-06-08 2022-12-09 广东福克药业科技有限公司 Application of serum albumin and mitochondrial agonist extracted from stem cells
FR3130290A1 (en) 2021-12-15 2023-06-16 L'oreal Dedifferentiated plant cells of the genus Paeonia, its extracts and its cosmetic uses

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CN105769653A (en) * 2016-03-01 2016-07-20 苏州艾缇克药物化学有限公司 SOD skin-caring composition with stable activity and preparing method thereof
CN106983713A (en) * 2017-03-31 2017-07-28 杭州哈佛赛尔干细胞技术有限公司 A kind of stem cell skin care composition and preparation method thereof and application method

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Publication number Priority date Publication date Assignee Title
CN105682635A (en) * 2013-08-09 2016-06-15 得凯莫斯公司弗罗里达有限公司 Skin care compositions having cyclic diesters and methods thereof
CN105769653A (en) * 2016-03-01 2016-07-20 苏州艾缇克药物化学有限公司 SOD skin-caring composition with stable activity and preparing method thereof
CN106983713A (en) * 2017-03-31 2017-07-28 杭州哈佛赛尔干细胞技术有限公司 A kind of stem cell skin care composition and preparation method thereof and application method

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CN106983713B (en) * 2017-03-31 2020-06-26 杭州哈佛赛尔干细胞技术有限公司 Stem cell skin care composition and preparation method and using method thereof
CN110522703A (en) * 2019-06-12 2019-12-03 青岛华澳现代医疗美容有限公司 The skin care compositions and preparation method with skin selfreparing anti-aging for micropin
CN110368357A (en) * 2019-08-13 2019-10-25 江苏安泰生物技术有限公司 A kind of skin nursing Essence and preparation method thereof
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