CN105769653A - SOD skin-caring composition with stable activity and preparing method thereof - Google Patents
SOD skin-caring composition with stable activity and preparing method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/925—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of animal origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention provides an SOD skin-caring composition with stable activity and a preparing method thereof.The preparing method includes the specific steps that 1, fresh aloe leaves and fresh garlic leaves are selected, chipped after surface impurity removing and added into phosphate buffer for ultrasonic treatment, the mixture is stirred to obtain an even solution, supernatant is taken after centrifuging separating, and crude enzyme filter liquor is obtained; 2, the crude enzyme filter liquor prepared in the step 1 is heated to be at 30 DEG C-35 DEG C, mannitol and cane sugar are added, the mixture is stirred and reacted for 20 min-30 min, rapidly cooled and filtered through a ultrafiltration membrane, and modified SOD enzyme liquor is obtained; 3, the modified SOD enzyme liquor prepared in the step 2 is sterilized at high temperature and added into a natural skin-caring composition, the mixture is stirred to be even, and the SOD skin-caring composition with stable activity is obtained.The preparing method of the SOD skin-caring composition with stable activity is easy and convenient, the activity of SOD prepared by combining the physical modification technique and the chemical modification technique is stable, effective ingredients can be provided for a long time, and the long-time skin caring aim can be achieved.
Description
Technical field
The invention belongs to amenities technical field, be specifically related to a kind of activity stabilized SOD skin care compositions and system thereof
Preparation Method.
Background technology
Along with improving constantly of living standard, people to the requirement of cosmetics the most increasingly toward high-level development, Ren Menyi
Through being unsatisfactory for simple skin-protection product, in addition it is also necessary to there is the skin-protection product of specific function, as dispelling stain, prevent wrinkle,
Keep elasticity of skin etc..
Superoxide dismutase SOD belongs to metalloenzyme, is present in animal, plant, algae and other prokaryotes more
In body, the amino acid sequence slightly difference purifying enzyme that separate sources extracts, therefore character the most difference.SOD's
Character depends not only on protein portion, and depends on the metal ion of bonding agent active site, according to the gold combined
The kind belonging to ion is different, is divided into copper zinc SOD, iron SOD and manganese SOD, and they can be by superoxide anion catalytic disproportionation
For hydrogen peroxide and oxygen.
According to the free-radical theory of skin senescence, in the aging course of skin, the infringement of radical pair skin is one
Important factor, the free radical of internal generation forms the materials such as MDA with aliphatic acid effect, and MDA is equal to cell membrane
On the effect such as protein form lipofuscin and be deposited on skin, form multiple color spot, and free radical also can make epidermis
Interior collagenous fibres, elastic fibers crosslinking and sex change, become fragile and follow the string.When the water deficient of skin, easily produce
Raw wrinkle.Therefore radical pair is the biggest in the detrimental effect of skin, and the free radical generated is had by needs
The elimination of effect, and reduce the generation of new free radical, and keep the moisture of skin, thus can effectively slow down skin
The aging of skin.
SOD is effective scavenger of superoxide anion free radical, but the SOD deposited at present easily inactivates.Chinese patent
CN
Disclosed in 101768582B (publication date 2010.7.7), a kind of SOD modifies production method, the S that will extract from animal blood
OD enzyme liquid, adds environment and the process of the chemical reaction of lauroyl chloride and aqueous slkali modification SOD, improves the steady of SOD activity
Qualitative, and without traditional acetone, it is ensured that the security of product and the activity of SOD.Chinese patent CN
The extracting method of a kind of SOD disclosed in 102747054B (publication date 2012.10.24), from the leaching liquor of Rosa roxburghii fruit powder
In be centrifuged isolated SOD crude enzyme liquid, obtain SOD solution through ultrafiltration, more condensing centrifugal spray drying obtain SOD
.The method whole process uses low-temperature physics gimmick, it is ensured that biologically active, and does not has environmental pollution and chemical contamination.
Have above-mentioned and have technology to understand, in order to improve the stability of SOD activity, the gimmick of physics or chemistry can be used to enter
OK, but using pure chemistry or physics gimmick modification SOD to have certain limitation, therefore, the present invention uses thing
Reason SOD modified with chemistry gimmick and combination, obtains activity stabilized SOD skin care item.
Summary of the invention:
The technical problem to be solved in the present invention is to provide a kind of activity stabilized SOD skin care compositions and preparation method thereof, will
The crude enzyme liquid extracted from fresh garlic and aloe, obtains activity stabilized SO after chemically and physically method is modified and purified
D, then SOD is introduced in skin care compositions, obtain activity stabilized SOD skin care compositions.
For solving above-mentioned technical problem, the technical scheme is that
A kind of activity stabilized SOD skin care compositions, it is characterised in that comprise the S of modification in described SOD skin care compositions
OD, the SOD of described modification are the SOD through activity stabilized modification, also comprise natural skin care in described SOD skin care compositions
Composition, also comprises the auxiliary agent that natural animal-plant extracts in described natural skin care composition, described auxiliary agent comprises NMF
, emollient, preservative and dispersant.
Preferably, described NMF is SP, and skin conditioner is lanolin, and described preservative is Tea Polyphenols, and dispersant is
Soybean protein surfactant.
The present invention also provides for the preparation method of a kind of activity stabilized SOD skin care compositions, it is characterised in that: include following
Step:
(1) choose fresh Aloe leave and garlic leaf, after removing the impurity on surface, be cut into small pieces, add phosphoric acid
In buffer solution, ultrasonic wave processes, and stirring obtains uniform solution, takes supernatant and obtain thick enzyme filtrate after centrifugation；
(2) thick enzyme filtrate prepared by step (1) is heated to 30-35 DEG C, adds mannitol and sucrose, stirring reaction
20-30min, rapidly after cooling, after ultrafiltration membrance filter, obtains the SOD enzyme liquid modified；
(3), after the SOD enzyme liquid high-temperature sterilization of modification prepared by step (2), add in natural skin care composition, stir
Mix uniformly, obtain activity stabilized SOD skin care compositions.
Preferred as technique scheme, in described step (1), Aloe leave is 1 with the mass ratio of garlic leaf:
0.1-10。
Preferred as technique scheme, in described step (1), the concentration of phosphate buffer is 0.06-0.08mol
/ L, pH value is 7.8.
Preferred as technique scheme, in described step (1), the power of ultrasonic wave is 250-300W, time ultrasonic
Between be 3-5min.
Preferred as technique scheme, in described step (2), thick enzyme filtrate and mannitol and the mass ratio of sucrose
For 50:1-2:0.5-2.
Preferred as technique scheme, in described step (2), the condition of ultrafiltration membrance filter is 30 DEG C, pressure 1.
5MPa, transmitance is 85-92%.
Preferred as technique scheme, in described step (3), in SOD skin care compositions, the content of SOD enzyme is 30
-50%.
Preferably, described activity stabilized SOD skin care compositions acceptable carrier is prepared as emulsion or face cream.
With prior art with than, the method have the advantages that
(1) main functional component in activity stabilized SOD skin care compositions prepared by the present invention is the SOD modified,
The preparation method of the SOD modified is easy, uses physics and chemical modification gimmick activity stabilized with the SOD combining preparation, can
Active ingredient is provided for a long time, reaches the purpose of lasting skin care.
(2) SOD of activity modifying prepared by the present invention selects phosphate buffer to extract and ultrasonic wave treatment technology and combination
Gimmick extract the thick enzyme of SOD, the release of intracellular matter can be increased fast and effectively, spread and dissolve, be extracted
The SOD active ingredient that rate is high, then processes via the chemically stable of mannitol and sucrose, obtains activity stabilized SOD.
(3) the SOD skincare composition that prepared by the present invention has also been selected the auxiliary agent extracted in natural animal-plant, has not selected
The chemical substance of Prof. Du Yucang, it is ensured that integral product green natural, environmental protection is effective.
Detailed description of the invention:
The present invention is described in detail, in illustrative examples and the explanation of this present invention below in conjunction with specific embodiment
It is used for explaining the present invention, but not as a limitation of the invention.
Embodiment 1:
(1) choose fresh Aloe leave and garlic leaf that mass ratio is 1:0.1, after removing the impurity on surface, cut
Become fritter, add 0.06mol/L
The phosphate buffer that pH value is 7.8 in, 250W ultrasonic wave processes 3min, and stirring obtains uniform solution, centrifugal point
Take supernatant after from and obtain the thick enzyme filtrate of 2.3mg/ml.
(2) by weight, the thick enzyme filtrate of 50 parts is heated to 30 DEG C, adds the mannitol of 1 part and the sugarcane of 0.5 part
Sugar, stirring reaction 20min, rapidly after cooling, under the pressure of 30 DEG C and 1.5MPa, after ultrafiltration membrance filter,
SOD enzyme liquid to the modification that transmitance is 85%.
(3) by NMF SP, skin conditioner lanolin, preservative Tea Polyphenols, dispersant soybean protein surfactant
After mixing, add the SOD enzyme liquid of the modification of high-temperature sterilization, stir, obtain activity stabilized SOD skin care group
Compound, wherein the content of SOD enzyme is 30%.
Embodiment 2:
(1) choosing mass ratio is 1:
The fresh Aloe leave of 10 and garlic leaf, after removing the impurity on surface, be cut into small pieces, and adds 0.08mol/L
The phosphate buffer that pH value is 7.8 in,
300W ultrasonic wave processes 5min, and stirring obtains uniform solution, takes supernatant and obtain the thick of 3.4mg/ml after centrifugation
Enzyme filtrate.
(2) by weight, the thick enzyme filtrate of 50 parts is heated to 35 DEG C, adds the mannitol of 2 parts and the sucrose of 2 parts
, stirring reaction 30min, rapidly after cooling, under the pressure of 30 DEG C and 1.5MPa, after ultrafiltration membrance filter, obtain
Transmitance is the SOD enzyme liquid of the modification of 92%.
(3) by NMF SP, skin conditioner lanolin, preservative Tea Polyphenols, dispersant soybean protein surfactant
After mixing, add the SOD enzyme liquid of the modification of high-temperature sterilization, stir, obtain activity stabilized SOD skin care group
Compound, wherein the content of SOD enzyme is 50%.
Embodiment 3:
(1) choose fresh Aloe leave and garlic leaf that mass ratio is 1:5, after removing the impurity on surface, be cut into
Fritter, adds 0.07mol/L
The phosphate buffer that pH value is 7.8 in, 250W ultrasonic wave processes 4min, and stirring obtains uniform solution, centrifugal point
Take supernatant after from and obtain the thick enzyme filtrate of 2.7mg/ml.
(2) by weight, the thick enzyme filtrate of 50 parts is heated to 30-35 DEG C, adds the mannitol of 1.5 parts and 1 part
Sucrose, stirring reaction 25min, rapidly after cooling, under the pressure of 30 DEG C and 1.5MPa, after ultrafiltration membrance filter
, obtain the SOD enzyme liquid of the modification that transmitance is 90%.
(3) by NMF SP, skin conditioner lanolin, preservative Tea Polyphenols, dispersant soybean protein surfactant
After mixing, add the SOD enzyme liquid of the modification of high-temperature sterilization, stir, obtain activity stabilized SOD skin care group
Compound, wherein the content of SOD enzyme is 35%.
Embodiment 4:
(1) choose fresh Aloe leave and garlic leaf that mass ratio is 1:1, after removing the impurity on surface, be cut into
Fritter, adds in the phosphate buffer that pH value is 7.8 of 0.06mol/L,
300W ultrasonic wave processes 4min, and stirring obtains uniform solution, takes supernatant and obtain the thick of 3.2mg/ml after centrifugation
Enzyme filtrate.
(2) by weight, the thick enzyme filtrate of 50 parts is heated to 30 DEG C, adds the mannitol of 1 part and the sugarcane of 1.5 parts
Sugar, stirring reaction 20min, rapidly after cooling, under the pressure of 30 DEG C and 1.5MPa, after ultrafiltration membrance filter,
SOD enzyme liquid to the modification that transmitance is 88%.
(3) by NMF SP, skin conditioner lanolin, preservative Tea Polyphenols, dispersant soybean protein surfactant
After mixing, add the SOD enzyme liquid of the modification of high-temperature sterilization, stir, obtain activity stabilized SOD skin care group
Compound, wherein the content of SOD enzyme is 40%.
Embodiment 5:
(1) choose fresh Aloe leave and garlic leaf that mass ratio is 1:3, after removing the impurity on surface, be cut into
Fritter, adds in the phosphate buffer that pH value is 7.8 of 0.08mol/L,
300W ultrasonic wave processes 3min, and stirring obtains uniform solution, takes supernatant and obtain the thick of 2.7mg/ml after centrifugation
Enzyme filtrate.
(2) by weight, the thick enzyme filtrate of 50 parts is heated to 35 DEG C, adds the mannitol of 1.2 parts and the sugarcane of 1 part
Sugar, stirring reaction 20min, rapidly after cooling, under the pressure of 30 DEG C and 1.5MPa, after ultrafiltration membrance filter,
SOD enzyme liquid to the modification that transmitance is 90%.
(3) by NMF SP, skin conditioner lanolin, preservative Tea Polyphenols, dispersant soybean protein surfactant
After mixing, add the SOD enzyme liquid of the modification of high-temperature sterilization, stir, obtain activity stabilized SOD skin care group
Compound, wherein the content of SOD enzyme is 45%.
Embodiment 6:
(1) choose fresh Aloe leave and garlic leaf that mass ratio is 1:0.2, after removing the impurity on surface, cut
Become fritter, add 0.06mol/L
The phosphate buffer that pH value is 7.8 in, 250W ultrasonic wave processes 3.5min, and stirring obtains uniform solution, centrifugal
Take supernatant after separation and obtain the thick enzyme filtrate of 2.5mg/ml.
(2) by weight, the thick enzyme filtrate of 50 parts is heated to 30 DEG C, adds the mannitol of 1.4 parts and 0.5 part
Sucrose, stirring reaction 20min, rapidly after cooling, under the pressure of 30 DEG C and 1.5MPa, after ultrafiltration membrance filter,
Obtain the SOD enzyme liquid of the modification that transmitance is 88%.
(3) by NMF SP, skin conditioner lanolin, preservative Tea Polyphenols, dispersant soybean protein surfactant
After mixing, add the SOD enzyme liquid of the modification of high-temperature sterilization, stir, obtain activity stabilized SOD skin care group
Compound, wherein the content of SOD enzyme is 38%.
Embodiment 7:
(1) choose fresh Aloe leave and garlic leaf that mass ratio is 1:0.6, after removing the impurity on surface, cut
Become fritter, add 0.07mol/L
The phosphate buffer that pH value is 7.8 in, 250W ultrasonic wave processes 3.5min, and stirring obtains uniform solution, centrifugal
Take supernatant after separation and obtain the thick enzyme filtrate of 2.3mg/ml.
(2) by weight, the thick enzyme filtrate of 50 parts is heated to 30 DEG C, adds the mannitol of 1.6 parts and 0.6 part
Sucrose, stirring reaction 20min, rapidly after cooling, under the pressure of 30 DEG C and 1.5MPa, after ultrafiltration membrance filter,
Obtain the SOD enzyme liquid of the modification that transmitance is 89%.
(3) by NMF SP, skin conditioner lanolin, preservative Tea Polyphenols, dispersant soybean protein surfactant
After mixing, add the SOD enzyme liquid of the modification of high-temperature sterilization, stir, obtain activity stabilized SOD skin care group
Compound, wherein the content of SOD enzyme is 42%.
Embodiment 8:
(1) choose fresh Aloe leave and garlic leaf that mass ratio is 1:0.8, after removing the impurity on surface, cut
Become fritter, add 0.08mol/L
The phosphate buffer that pH value is 7.8 in, 250W ultrasonic wave processes 3.5min, and stirring obtains uniform solution, centrifugal
Take supernatant after separation and obtain the thick enzyme filtrate of 2.9mg/ml.
(2) by weight, the thick enzyme filtrate of 50 parts is heated to 30 DEG C, adds the mannitol of 1.9 parts and 0.8 part
Sucrose, stirring reaction 20min, rapidly after cooling, under the pressure of 30 DEG C and 1.5MPa, after ultrafiltration membrance filter,
Obtain the SOD enzyme liquid of the modification that transmitance is 92%.
(3) by NMF SP, skin conditioner lanolin, preservative Tea Polyphenols, dispersant soybean protein surfactant
After mixing, add the SOD enzyme liquid of the modification of high-temperature sterilization, stir, obtain activity stabilized SOD skin care group
Compound, wherein the content of SOD enzyme is 47%.
After testing, enzyme activity after 0h, 1h and 4h of activity stabilized SOD skin care compositions prepared by embodiment 1-8
Result is as follows:
As seen from the above table, the enzyme activity of activity stabilized SOD skin care compositions prepared by the present invention is high, and long-time enzyme is lived
Power is stable, can keep activity for a long time, effectively moistens and protection skin.
The principle of above-described embodiment only illustrative present invention and effect thereof, not for limiting the present invention.Any ripe
Above-described embodiment all can be modified under the spirit and the scope of the present invention or change by the personage knowing this technology.
Therefore, art has usually intellectual such as without departing from disclosed spirit and technological thought
Lower all completed equivalences are modified or change, and must be contained by the claim of the present invention.
Claims (10)
1. an activity stabilized SOD skin care compositions, it is characterised in that comprise modification in described SOD skin care compositions
SOD, the SOD of described modification be the SOD through activity stabilized modification, described SOD skin care compositions also comprises natural protecting
Skin composition, also comprises the auxiliary agent that natural animal-plant extracts in described natural skin care composition, described auxiliary agent comprises moisturizing
Agent, emollient, preservative and dispersant.
A kind of activity stabilized SOD skin care compositions the most according to claim 1, it is characterised in that described moisturizing
Agent is SP, and skin conditioner is lanolin, and described preservative is Tea Polyphenols, and dispersant is soybean protein surfactant.
3. the preparation method of an activity stabilized SOD skin care compositions, it is characterised in that: comprise the following steps:
(1) choose fresh Aloe leave and garlic leaf, after removing the impurity on surface, be cut into small pieces, add phosphoric acid
In buffer solution, ultrasonic wave processes, and stirring obtains uniform solution, takes supernatant and obtain thick enzyme filtrate after centrifugation；
(2) thick enzyme filtrate prepared by step (1) is heated to 30-35 DEG C, adds mannitol and sucrose, stirring reaction
20-30min, rapidly after cooling, after ultrafiltration membrance filter, obtains the SOD enzyme liquid modified；
(3), after the SOD enzyme liquid high-temperature sterilization of modification prepared by step (2), add in natural skin care composition, stir
Mix uniformly, obtain activity stabilized SOD skin care compositions.
The preparation method of a kind of activity stabilized SOD skin care compositions the most according to claim 3, it is characterised in that
: in described step (1), Aloe leave is 1:0.1-10 with the mass ratio of garlic leaf.
The preparation method of a kind of activity stabilized SOD skin care compositions the most according to claim 3, it is characterised in that
: in described step (1), the concentration of phosphate buffer is 0.06-0.08mol/L, and pH value is 7.8.
The preparation method of a kind of activity stabilized SOD skin care compositions the most according to claim 3, it is characterised in that
: in described step (1), the power of ultrasonic wave is 250-300W, and ultrasonic time is 3-5min.
The preparation method of a kind of activity stabilized SOD skin care compositions the most according to claim 3, it is characterised in that
: in described step (2), thick enzyme filtrate is 50:1-2:0.5-2 with the mass ratio of mannitol and sucrose.
The preparation method of a kind of activity stabilized SOD skin care compositions the most according to claim 3, it is characterised in that
: in described step (2), the condition of ultrafiltration membrance filter is 30 DEG C, pressure 1.5MPa, and transmitance is 85-92%.
The preparation method of a kind of activity stabilized SOD skin care compositions the most according to claim 3, it is characterised in that
: in described step (3), in SOD skin care compositions, the content of SOD enzyme is 30-50%.
A kind of activity stabilized SOD skin care compositions the most according to claim 1 and 2, it is characterised in that:
Described activity stabilized SOD skin care compositions acceptable carrier is prepared as emulsion or face cream.
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| CN108434010A (en) * | 2018-06-06 | 2018-08-24 | 西南交通大学 | A kind of the pickering emulsion type cosmetics and its preparation process of SOD enzyme granulates stabilization |
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| CN117126822A (en) * | 2023-10-27 | 2023-11-28 | 山东爱维德生物科技有限公司 | Adamantine modified SOD enzyme for arborescent aloe SOD composite pure dew and application thereof |
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| CN107951820A (en) * | 2017-12-01 | 2018-04-24 | 重庆金时代生物技术有限公司 | A kind of stem cell skin care composition and preparation method thereof |
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| CN117126822B (en) * | 2023-10-27 | 2023-12-22 | 山东爱维德生物科技有限公司 | Adamantine modified SOD enzyme for arborescent aloe SOD composite pure dew and application thereof |
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