CN107935855A - A kind of technique that Rosmarinic acid is extracted from rosemary - Google Patents
A kind of technique that Rosmarinic acid is extracted from rosemary Download PDFInfo
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- CN107935855A CN107935855A CN201810008902.8A CN201810008902A CN107935855A CN 107935855 A CN107935855 A CN 107935855A CN 201810008902 A CN201810008902 A CN 201810008902A CN 107935855 A CN107935855 A CN 107935855A
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- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
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Abstract
The present invention relates to extraction process technology field, especially a kind of technique that Rosmarinic acid is extracted from rosemary, comprises the following steps：S1, immersion：It is powdery that rosemary, which crushes, is then soaked using ethanol；S2, extraction：The rosemary of powdery is extracted with alcohol reflux, and an eluent is obtained after alcohol is washed；S3, absorption：By an eluent by NK109 resin adsorptions, secondary eluent is obtained after alcohol is washed；S4, crystallization：Then secondary eluent is subjected to active rock decoloration, is extracted repeatedly using extractant 46 times, take extraction oxidant layer to carry out low temperature crystallization and recrystallization；S5, drying：Rosmarinic acid will can be obtained after crystal drying obtained by previous step.The relatively traditional Rosmarinic acid extracting method of the present invention, method are easy, it is easy to accomplish, and extracted amount is big, and safe and non-toxic, Product quality and safety is protected and can effectively reduce cost.
Description
Technical field
The present invention relates to extraction process technology field, more particularly to a kind of technique that Rosmarinic acid is extracted from rosemary.
Background technology
Rosemary is that Labiatae fan changes platymiscium herb, is generally acknowledged at present to have compared with high anti-oxidation effect and safe and non-toxic
A kind of plant.Rosmarinic acid is as one of most effective antioxidant content in rosemary, by Food and Drug Administration
(FDA) it is recognized as " public security food ".Rosmarinic acid, entitled [R (the E)-α-[[3- (3,4- dihydroxy phenyl) -1- oxygen of chemistry
Generation -2- acrylic] epoxide] -3,4- dihydroxy benzenes propionic acid, it is a kind of multi-functional phenolic acid compound, structural instability, to light
According to, temperature, metal ion etc. it is all more sensitive.There is very strong anti-oxidant, anti-inflammatory, antibacterial, antiviral, immunosupress etc. to make for it
With being had broad application prospects in food, cosmetics, medicine etc..Traditional Rosmarinic acid extracting method deposits standard deviation
Greatly, extracted amount is few, extract it is molten Ji poisonous, Product quality and safety without ensure, it is of high cost the problems such as.
The content of the invention
The purpose of the present invention is to solve shortcoming existing in the prior art, and the one kind proposed is extracted from rosemary
The technique of Rosmarinic acid.
To achieve these goals, present invention employs following technical solution：
A kind of technique that Rosmarinic acid is extracted from rosemary is designed, is comprised the following steps：
S1, immersion：It is powdery that rosemary, which is added pulverizer and crushed, is then soaked using ethanol；
S2, extraction：The rosemary of powdery after immersion obtained by previous step is extracted with alcohol reflux, extracting solution passes through tree
After fat absorption, an eluent is obtained after alcohol is washed；
S3, absorption：By an eluent by NK109 resin adsorptions, it is pure that the NK109 resins of Static Adsorption can improve extraction
Degree, obtains secondary eluent after alcohol is washed；
S4, crystallization：Then secondary eluent is subjected to active rock decoloration, is extracted 4-6 times repeatedly using extractant, take extraction
Oxidant layer is taken to carry out low temperature crystallization and recrystallization；
S5, drying：Rosmarinic acid will can be obtained after crystal drying obtained by previous step.
Preferably, the volume fraction of ethanol is 65-75%, and soaking time continues 1-2h.
Preferably, in the decolorization of S4, destainer pH value holding 4-6 is adjusted by adding citric acid.
Preferably, extractant is ethyl acetate.
Preferably, it is necessary to reach 45 mesh after rosemary crushes in S1.
Preferably, extraction process control temperature is at 50-70 DEG C in S2, extraction time 70-90min.
A kind of technique that Rosmarinic acid is extracted from rosemary proposed by the present invention, beneficial effect are：It is of the invention opposite
Traditional Rosmarinic acid extracting method, method are easy, it is easy to accomplish, and extracted amount is big, and safe and non-toxic, Product quality and safety obtains
Ensure and can effectively reduce cost.
Embodiment
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, instead of all the embodiments.
Embodiment 1
A kind of technique that Rosmarinic acid is extracted from rosemary, comprises the following steps：
S1, immersion：It is powdery that rosemary, which is added pulverizer and crushed, is then soaked using ethanol, after rosemary crushes, is needed
Reach 45 mesh；
S2, extraction：The rosemary of powdery after immersion obtained by previous step is extracted with alcohol reflux, extracting solution passes through tree
After fat absorption, an eluent is obtained after alcohol is washed, the volume fraction of ethanol is 65%, and soaking time continues 1h, controls temperature
At 50 DEG C, extraction time 70min；
S3, absorption：By an eluent by NK109 resin adsorptions, it is pure that the NK109 resins of Static Adsorption can improve extraction
Degree, obtains secondary eluent after alcohol is washed；
S4, crystallization：Then secondary eluent is carried out active rock to decolourize, in decolorization, by adding citric acid tune
Save destainer pH value and keep 4, extracted repeatedly using extractant 4 times, take extraction oxidant layer to carry out low temperature crystallization and recrystallization, extractant
For ethyl acetate；
S5, drying：Rosmarinic acid will can be obtained after crystal drying obtained by previous step.
Embodiment 2
A kind of technique that Rosmarinic acid is extracted from rosemary, comprises the following steps：
S1, immersion：It is powdery that rosemary, which is added pulverizer and crushed, is then soaked using ethanol, after rosemary crushes, is needed
Reach 45 mesh；
S2, extraction：The rosemary of powdery after immersion obtained by previous step is extracted with alcohol reflux, extracting solution passes through tree
After fat absorption, an eluent is obtained after alcohol is washed, the volume fraction of ethanol is 66%, and soaking time continues 1h, controls temperature
At 55 DEG C, extraction time 75min；
S3, absorption：By an eluent by NK109 resin adsorptions, it is pure that the NK109 resins of Static Adsorption can improve extraction
Degree, obtains secondary eluent after alcohol is washed；
S4, crystallization：Then secondary eluent is carried out active rock to decolourize, in decolorization, by adding citric acid tune
Save destainer pH value and keep 5, extracted repeatedly using extractant 5 times, take extraction oxidant layer to carry out low temperature crystallization and recrystallization, extractant
For ethyl acetate；
S5, drying：Rosmarinic acid will can be obtained after crystal drying obtained by previous step.
Embodiment 3
A kind of technique that Rosmarinic acid is extracted from rosemary, comprises the following steps：
S1, immersion：It is powdery that rosemary, which is added pulverizer and crushed, is then soaked using ethanol, after rosemary crushes, is needed
Reach 45 mesh；
S2, extraction：The rosemary of powdery after immersion obtained by previous step is extracted with alcohol reflux, extracting solution passes through tree
After fat absorption, an eluent is obtained after alcohol is washed, the volume fraction of ethanol is 70%, and soaking time continues 2h, controls temperature
At 60 DEG C, extraction time 85min；
S3, absorption：By an eluent by NK109 resin adsorptions, it is pure that the NK109 resins of Static Adsorption can improve extraction
Degree, obtains secondary eluent after alcohol is washed；
S4, crystallization：Then secondary eluent is carried out active rock to decolourize, in decolorization, by adding citric acid tune
Save destainer pH value and keep 5, extracted repeatedly using extractant 5 times, take extraction oxidant layer to carry out low temperature crystallization and recrystallization, extractant
For ethyl acetate；
S5, drying：Rosmarinic acid will can be obtained after crystal drying obtained by previous step.
Embodiment 4
A kind of technique that Rosmarinic acid is extracted from rosemary, comprises the following steps：
S1, immersion：It is powdery that rosemary, which is added pulverizer and crushed, is then soaked using ethanol, after rosemary crushes, is needed
Reach 45 mesh；
S2, extraction：The rosemary of powdery after immersion obtained by previous step is extracted with alcohol reflux, extracting solution passes through tree
After fat absorption, an eluent is obtained after alcohol is washed, the volume fraction of ethanol is 75%, and soaking time continues 2h, controls temperature
At 70 DEG C, extraction time 90min；
S3, absorption：By an eluent by NK109 resin adsorptions, it is pure that the NK109 resins of Static Adsorption can improve extraction
Degree, obtains secondary eluent after alcohol is washed；
S4, crystallization：Then secondary eluent is carried out active rock to decolourize, in decolorization, by adding citric acid tune
Save destainer pH value and keep 6, extracted repeatedly using extractant 6 times, take extraction oxidant layer to carry out low temperature crystallization and recrystallization, extractant
For ethyl acetate；
S5, drying：Rosmarinic acid will can be obtained after crystal drying obtained by previous step.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of technique that Rosmarinic acid is extracted from rosemary, it is characterised in that comprise the following steps：
S1, immersion：It is powdery that rosemary, which is added pulverizer and crushed, is then soaked using ethanol；
S2, extraction：The rosemary of powdery after immersion obtained by previous step is extracted with alcohol reflux, extracting solution is inhaled by resin
It is attached, an eluent is obtained after alcohol is washed；
S3, absorption：Eluent can be improved into DNA purity by NK109 resin adsorptions, the NK109 resins of Static Adsorption,
Secondary eluent is obtained after alcohol is washed；
S4, crystallization：Then secondary eluent is subjected to active rock decoloration, is extracted 4-6 times repeatedly using extractant, take extractant
Layer carries out low temperature crystallization and recrystallization；
S5, drying：Rosmarinic acid will can be obtained after crystal drying obtained by previous step.
A kind of 2. technique that Rosmarinic acid is extracted from rosemary according to claim 1, it is characterised in that：The body of ethanol
Fraction is 65-75%, and soaking time continues 1-2h.
A kind of 3. technique that Rosmarinic acid is extracted from rosemary according to claim 1, it is characterised in that：The decoloration of S4
During, adjust destainer pH value holding 4-6 by adding citric acid.
A kind of 4. technique that Rosmarinic acid is extracted from rosemary according to claim 1, it is characterised in that：Extractant is
Ethyl acetate.
A kind of 5. technique that Rosmarinic acid is extracted from rosemary according to claim 1, it is characterised in that：Fan changes in S1
, it is necessary to reach 45 mesh after face powder is broken.
A kind of 6. technique that Rosmarinic acid is extracted from rosemary according to claim 1, it is characterised in that：Extracted in S2
Process control temp is at 50-70 DEG C, extraction time 70-90min.
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| CN201810008902.8A CN107935855A (en) | 2018-01-04 | 2018-01-04 | A kind of technique that Rosmarinic acid is extracted from rosemary |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108658769A (en) * | 2018-04-26 | 2018-10-16 | 铜仁职业技术学院 | One kind being based on response phase method Prunella vulgaris Rosmarinic acid extracting method |
| CN110201027A (en) * | 2019-07-02 | 2019-09-06 | 晨光生物科技集团股份有限公司 | A kind of water-soluble Lamiaceae plant extract and preparation method thereof with raising |
| WO2020258896A1 (en) | 2019-06-25 | 2020-12-30 | 陕西鸿道生物分析科学技术研究院有限公司 | Strain and method for producing rosmarinic acid |
| CN112915040A (en) * | 2021-01-28 | 2021-06-08 | 晨光生物科技集团股份有限公司 | Rosemary extract with low ash content and high water solubility and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101863767A (en) * | 2010-03-23 | 2010-10-20 | 南京泽朗农业发展有限公司 | Preparation process of rosmarinic acid |
| US20100305366A1 (en) * | 2008-10-31 | 2010-12-02 | Juan Liu | Lutein Extraction Processes |
| CN106565489A (en) * | 2016-10-10 | 2017-04-19 | 广西来宾绿翔生物科技有限公司 | Process for extracting rosmarinic acid from perilla |
-
2018
- 2018-01-04 CN CN201810008902.8A patent/CN107935855A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100305366A1 (en) * | 2008-10-31 | 2010-12-02 | Juan Liu | Lutein Extraction Processes |
| CN101863767A (en) * | 2010-03-23 | 2010-10-20 | 南京泽朗农业发展有限公司 | Preparation process of rosmarinic acid |
| CN106565489A (en) * | 2016-10-10 | 2017-04-19 | 广西来宾绿翔生物科技有限公司 | Process for extracting rosmarinic acid from perilla |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108658769A (en) * | 2018-04-26 | 2018-10-16 | 铜仁职业技术学院 | One kind being based on response phase method Prunella vulgaris Rosmarinic acid extracting method |
| WO2020258896A1 (en) | 2019-06-25 | 2020-12-30 | 陕西鸿道生物分析科学技术研究院有限公司 | Strain and method for producing rosmarinic acid |
| CN110201027A (en) * | 2019-07-02 | 2019-09-06 | 晨光生物科技集团股份有限公司 | A kind of water-soluble Lamiaceae plant extract and preparation method thereof with raising |
| CN112915040A (en) * | 2021-01-28 | 2021-06-08 | 晨光生物科技集团股份有限公司 | Rosemary extract with low ash content and high water solubility and preparation method thereof |
| CN112915040B (en) * | 2021-01-28 | 2023-12-08 | 晨光生物科技集团股份有限公司 | Rosemary extract with low ash content and high water solubility and preparation method thereof |
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