CN107929446A - A kind of method that plant skin element is extracted from banana skin - Google Patents
A kind of method that plant skin element is extracted from banana skin Download PDFInfo
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- CN107929446A CN107929446A CN201711233084.3A CN201711233084A CN107929446A CN 107929446 A CN107929446 A CN 107929446A CN 201711233084 A CN201711233084 A CN 201711233084A CN 107929446 A CN107929446 A CN 107929446A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a kind of method that plant skin element is extracted from banana skin, it is related to the extractive technique fields of natural materials.The method of plant skin element is extracted in the slave banana skin of the present invention to be included：Dry banana skin is taken, methanol, acetic acid and the mixed solution of tetrahydrofuran and water is added and is heated to reflux for Extraction solvent；After cooling, the first filtrate and the first filter residue are filtered to take；Ammonium sulfate is added into the first filtrate, is staticly settled, filters to take the second filtrate；Absolute ethyl alcohol is added into the second filtrate, is staticly settled, filters to take precipitation；Precipitation is washed with absolute ethyl alcohol and ether respectively to obtain the final product.The simple and practicable cost of method of the extraction plant skin element provided by the invention from banana skin is low, it uses double solvents to extract banana skin, the extraction efficiency of plant skin element is high, provides a kind of new approach for the recycling of banana skin, and provide basis for the extensive use of plant skin element.
Description
Technical field
The present invention relates to natural materials extractive technique field, especially a kind of method that plant skin element is extracted from banana skin.
Background technology
Banana is one of four big fruit in the world, be the fruit of volume of world trade maximum, and global consumption figure is most
Fruit.In China, banana is the fourth-largest fruit occupied after apple, grape and citrus.Banana is due to intolerant to accumulating, often resulting in
Heavy losses after adopting, therefore some banana main production countries reduce damage by source establishing deep processing enterprise production banana food
Lose, at the same time produce the banana skin accessory substance of intimate fruit weight 35% or so.Such as abandoned as discarded object in field,
Not only waste of resource but also environment had been polluted, while can have also propagated some latency germs, be negatively affected to production.Therefore, develop
Using the food byproducts such as banana skin and discarded object, can protecting ecology can improve the economic benefits of food industries again, contain huge
Big market potential.
Contain abundant nutriment, such as protein, fat, sugar, mineral matter and trace element in banana.Wherein,
Plant skin element can effectively suppress cutaneous pruritus caused by fungi and bacterial growth breeding, treatment tinea pedis, the tinea manuum, ringworm of the body etc., and
Phenomena such as skin aging, decortication, itch, chap can be resisted.But do not have in ripe slave banana skin extract in the prior art
The method of plant skin element, limits its development and application.
The content of the invention
The goal of the invention of the present invention is：For above-mentioned problem, there is provided one kind extracts plant skin element from banana skin
Method, this method can expeditiously extract plant skin element from banana skin, promote the recycling of banana skin, and improve any of several broadleaf plants skin
The extraction efficiency of element, basis is provided for the extensive use of plant skin element, meanwhile, the simple and practicable cost of this method is low.
The technical solution adopted by the present invention is as follows：
A kind of method that plant skin element is extracted from banana skin, it includes the following steps：
Step 1：Dry banana skin is taken, it is Extraction solvent to add methanol, acetic acid and the mixed solution of tetrahydrofuran and water
It is heated to reflux；After cooling, the first filtrate and the first filter residue are filtered to take；
Step 2：Ammonium sulfate is added into the first filtrate, is staticly settled, filters to take the second filtrate；
Step 3：Absolute ethyl alcohol is added into the second filtrate, is staticly settled, filters to take precipitation；
Step 4：Precipitation is washed with absolute ethyl alcohol and ether respectively, to obtain the final product.
By adopting the above-described technical solution, plant skin element is in methanol, acetic acid and tetrahydrofuran and the mixed solution of water
Middle solubility is high, efficiently can always extract plant skin element from banana skin.Adding metabisulfite solution can be by soluble egg
It is precipitated out in vain.Plant skin element dissolubility in the ethanol of high concentration is low, is thus precipitated out.
A kind of method that in the present invention extracts plant skin element from banana skin, in step 1, methanol, acetic acid and tetrahydrofuran
Volume ratio between water is 0.4-0.6:0.3-0.5:0.2-0.3:1.
By adopting the above-described technical solution, plant skin element solubility in the Extraction solvent of the composition of aforementioned proportion is high, carry
Take efficient.
A kind of method that in the present invention extracts plant skin element from banana skin, the dry banana skin and the Extraction solvent it
Between solid-liquid ratio be 1:6-9（kg：L）.
A kind of method that plant skin element is extracted from banana skin in the present invention, it is 2-4h to be heated to reflux the time.
By adopting the above-described technical solution, extraction efficiency is high.
A kind of method that in the present invention extracts plant skin element from banana skin, in Extraction solvent described in the forward direction being heated to reflux
Tween 80 is added, is 0.2-0.6% to its mass concentration in Extraction solvent.
By adopting the above-described technical solution, Tween 80 is a kind of surfactant, it is possible to increase the permeability of cell, carries
The dissolution rate of high plant skin element, so as to improve recovery rate.
A kind of method that in the present invention extracts plant skin element from banana skin, in step 2, adds before ammonium sulfate,
The first filtrate is first subjected to concentrated by rotary evaporation to the 1/4-1/3 of the first filtrate, during revolving, bath temperature is 50-70 DEG C.
By adopting the above-described technical solution, on the one hand vaporing away the low-boiling organic solvent in part, reduce plant skin element and exist
Solubility in Extraction solvent, separates out easy to it, on the other hand can reduce the dosage of ammonium sulfate and absolute ethyl alcohol, saves
Cost.
A kind of method that plant skin element is extracted from banana skin in the present invention, according to the volume of concentrate and ammonium sulfate
Than for 1:The ratio of 1-2 adds the ammonium sulfate that mass percent is 46%-70% into concentrate.
By adopting the above-described technical solution, eduction rate is high in ammonium sulfate of the protein under the concentration, easy to protein
Complete precipitation.
A kind of method that in the present invention extracts plant skin element from banana skin, in step 3, nothing is added into the second filtrate
Water-ethanol is untill the percentage by volume of ethanol is 70-90%.
By adopting the above-described technical solution, solubility is told somebody what one's real intentions are in ethanol of the plant skin element under the concentration, easy to plant skin element
Complete precipitation, so as to isolate plant skin element.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. using the mixed solution of methanol, acetic acid and tetrahydrofuran and water as Extraction solvent, extraction efficiency is high.
2. before refluxing extraction, Tween 80 is added, the permeability of cell is improved, further improves extraction efficiency.
3. in extraction process, protein is removed using ammonium sulfate precipitation, and with the ethanol precipitation plant skin element of certain concentration, is carried
The plant skin element impurity content taken is low.
4. having effectively facilitated the recycling of banana skin, and provide the foundation for the commercial application of plant skin element.
Embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.
This specification（Including any accessory claim, summary）Disclosed in any feature, unless specifically stated,
Replaced by other equivalent or with similar purpose alternative features.I.e., unless specifically stated, each feature is a series of
An example in equivalent or similar characteristics.
Embodiment 1
The present embodiment provides a kind of method that plant skin element is extracted from banana skin, this method carries out banana skin using double solvents
Extraction, expeditiously extracts plant skin element, and a kind of new approach, and extensively should for plant skin element are provided for the recycling of banana skin
It is basic with providing.The method provided in this embodiment that plant skin element is extracted from banana skin includes the following steps：
Step 1：Take dry banana skin, it is 0.2-0.5mm to be crushed to particle diameter, add with enter methanol, acetic acid and tetrahydrofuran and
Water is 0.4 according to volume ratio:0.5:0.2:1 ratio mixes mixed solution and is heated to reflux 2h for Extraction solvent, its
In, the solid-liquid ratio between banana skin and Extraction solvent is 1:6.After reflux, be cooled to room temperature, be obtained by filtration the first filtrate and
First filter residue.
Step 2：It is 1 according to the volume ratio of ammonium sulfate and the first filtrate:1 adds mass fraction into the first filtrate
For 70% ammonium sulfate, after stirring evenly, 2h is staticly settled, filters to take the second filtrate.
Step 3：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 70%, is stirred evenly, and it is heavy to stand
Shallow lake 2h, filters to take precipitation.
Step 4：Precipitation is successively washed twice respectively with absolute ethyl alcohol and ether, is then drying to obtain at 60 DEG C.
Embodiment 2
The present embodiment provides a kind of method that plant skin element is extracted from banana skin, this method carries out banana skin using double solvents
Extraction, expeditiously extracts plant skin element, and a kind of new approach, and extensively should for plant skin element are provided for the recycling of banana skin
It is basic with providing.The method provided in this embodiment that plant skin element is extracted from banana skin includes the following steps：
Step 1：Take dry banana skin, it is 0.2-0.5mm to be crushed to particle diameter, add with enter methanol, acetic acid and tetrahydrofuran and
Water is 0.5 according to volume ratio:0.4:0.4:It is Extraction solvent that 1 ratio, which mixes mixed solution, and to Extraction solvent
Middle addition Tween 80 to its mass concentration in Extraction solvent is 0.2%, is heated to reflux 3h, wherein, banana skin and Extraction solvent
Between solid-liquid ratio be 1:8.After reflux, it is cooled to room temperature, the first filtrate and the first filter residue is obtained by filtration.
Step 2：Under conditions of bath temperature is 50 DEG C, by the first filtrate concentrated by rotary evaporation to the 1/4 of original volume, obtain
Concentrate.It is 1 according to the volume ratio of ammonium sulfate and concentrate:2 add the ammonium sulfate that mass fraction is 46% into concentrate
Solution, after stirring evenly, staticly settles 2h, filters to take the second filtrate.
Step 3：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 80%, is stirred evenly, and it is heavy to stand
Shallow lake 2h, filters to take precipitation.
Step 4：Precipitation is successively washed twice respectively with absolute ethyl alcohol and ether, is then drying to obtain at 60 DEG C.
Embodiment 3
The present embodiment provides a kind of method that plant skin element is extracted from banana skin, this method carries out banana skin using double solvents
Extraction, expeditiously extracts plant skin element, and a kind of new approach, and extensively should for plant skin element are provided for the recycling of banana skin
It is basic with providing.The method provided in this embodiment that plant skin element is extracted from banana skin includes the following steps：
Step 1：Take dry banana skin, it is 0.2-0.5mm to be crushed to particle diameter, add with enter methanol, acetic acid and tetrahydrofuran and
Water is 0.6 according to volume ratio:0.3:0.2:It is Extraction solvent that 1 ratio, which mixes mixed solution, and to Extraction solvent
Middle addition Tween 80 to its mass concentration in Extraction solvent is 0.4%, is heated to reflux 4h, wherein, banana skin and Extraction solvent
Between solid-liquid ratio be 1:9.After reflux, it is cooled to room temperature, the first filtrate and the first filter residue is obtained by filtration.
Step 2：Under conditions of bath temperature is 60 DEG C, by the first filtrate concentrated by rotary evaporation to the 1/3 of original volume, obtain
Concentrate.It is 1 according to the volume ratio of ammonium sulfate and concentrate:1 adds the ammonium sulfate that mass fraction is 68% into concentrate
Solution, after stirring evenly, staticly settles 2h, filters to take the second filtrate.
Step 3：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 90%, is stirred evenly, and it is heavy to stand
Shallow lake 2h, filters to take precipitation.
Step 4：Precipitation is successively washed twice respectively with absolute ethyl alcohol and ether, is then drying to obtain at 60 DEG C.
Embodiment 4
The present embodiment provides a kind of method that plant skin element is extracted from banana skin, this method carries out banana skin using double solvents
Extraction, expeditiously extracts plant skin element, and a kind of new approach, and extensively should for plant skin element are provided for the recycling of banana skin
It is basic with providing.The method provided in this embodiment that plant skin element is extracted from banana skin includes the following steps：
Step 1：Take dry banana skin, it is 0.2-0.5mm to be crushed to particle diameter, add with enter methanol, acetic acid and tetrahydrofuran and
Water is 0.5 according to volume ratio:0.5:0.3:It is Extraction solvent that 1 ratio, which mixes mixed solution, and to Extraction solvent
Middle addition Tween 80 to its mass concentration in Extraction solvent is 0.6%, is heated to reflux 2h, wherein, banana skin and Extraction solvent
Between solid-liquid ratio be 1:7.After reflux, it is cooled to room temperature, the first filtrate and the first filter residue is obtained by filtration.
Step 2：Under conditions of bath temperature is 70 DEG C, by the first filtrate concentrated by rotary evaporation to the 1/3 of original volume, obtain
Concentrate.It is 1 according to the volume ratio of ammonium sulfate and concentrate:2 add the ammonium sulfate that mass fraction is 54% into concentrate
Solution, after stirring evenly, staticly settles 2h, filters to take the second filtrate.
Step 3：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 80%, is stirred evenly, and it is heavy to stand
Shallow lake 2h, filters to take precipitation.
Step 4：Precipitation is successively washed twice respectively with absolute ethyl alcohol and ether, is then drying to obtain at 60 DEG C.
The invention is not limited in foregoing embodiment.The present invention, which expands to, any in the present specification to be disclosed
New feature or any new combination, and disclose any new method or process the step of or any new combination.
Claims (8)
- A kind of 1. method that plant skin element is extracted from banana skin, it is characterised in that it includes the following steps：Step 1：Dry banana skin is taken, it is Extraction solvent to add methanol, acetic acid and the mixed solution of tetrahydrofuran and water It is heated to reflux；After cooling, the first filtrate and the first filter residue are filtered to take；Step 2：Ammonium sulfate is added into the first filtrate, is staticly settled, filters to take the second filtrate；Step 3：Absolute ethyl alcohol is added into the second filtrate, is staticly settled, filters to take precipitation；Step 4：Precipitation is washed with absolute ethyl alcohol and ether respectively, to obtain the final product.
- 2. the method according to claim 1 that plant skin element is extracted from banana skin, it is characterised in that in step 1, methanol, Volume ratio between acetic acid and tetrahydrofuran and water is 0.4-0.6:0.3-0.5:0.2-0.3:1.
- 3. it is according to claim 2 from banana skin extract plant skin element method, it is characterised in that the dry banana skin with Solid-liquid ratio between the Extraction solvent is 1:6-9（kg：L）.
- 4. the method for plant skin element is extracted in the slave banana skin according to right will go 3, it is characterised in that being heated to reflux the time is 2-4h。
- 5. the method for plant skin element is extracted in the slave banana skin according to any one of right 1-4, it is characterised in that be heated to reflux Forward direction described in add Tween 80 in Extraction solvent, be 0.2-0.6% to its mass concentration in Extraction solvent.
- 6. the method according to claim 1 that plant skin element is extracted from banana skin, it is characterised in that in step 2, add Before ammonium sulfate, the 1/4-1/3 that the first filtrate is first carried out to concentrated by rotary evaporation to the first filtrate obtains concentrate, during revolving, Bath temperature is 50-70 DEG C.
- 7. the method according to claim 6 that plant skin element is extracted from banana skin, it is characterised in that according to concentrate and sulphur The volume ratio of acid ammonium solution is 1:The ratio of 1-2 adds the ammonium sulfate that mass percent is 46%-70% into concentrate.
- 8. the method according to claim 1 that plant skin element is extracted from banana skin, it is characterised in that in step 3, to the Absolute ethyl alcohol is added in two filtrates untill the percentage by volume of ethanol is 70-90%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711233084.3A CN107929446A (en) | 2017-11-30 | 2017-11-30 | A kind of method that plant skin element is extracted from banana skin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711233084.3A CN107929446A (en) | 2017-11-30 | 2017-11-30 | A kind of method that plant skin element is extracted from banana skin |
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| CN107929446A true CN107929446A (en) | 2018-04-20 |
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| CN201711233084.3A Withdrawn CN107929446A (en) | 2017-11-30 | 2017-11-30 | A kind of method that plant skin element is extracted from banana skin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113575622A (en) * | 2021-08-27 | 2021-11-02 | 烟台凯多海洋生物研究院有限公司 | Preparation and application methods of banana peel-derived mite repellent |
-
2017
- 2017-11-30 CN CN201711233084.3A patent/CN107929446A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113575622A (en) * | 2021-08-27 | 2021-11-02 | 烟台凯多海洋生物研究院有限公司 | Preparation and application methods of banana peel-derived mite repellent |
| CN113575622B (en) * | 2021-08-27 | 2022-08-02 | 烟台固特丽生物科技股份有限公司 | Preparation and application methods of banana peel-derived mite repellent |
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