CN107827806A - A kind of method that thrombocytin is extracted from banana skin - Google Patents
A kind of method that thrombocytin is extracted from banana skin Download PDFInfo
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- CN107827806A CN107827806A CN201711233264.1A CN201711233264A CN107827806A CN 107827806 A CN107827806 A CN 107827806A CN 201711233264 A CN201711233264 A CN 201711233264A CN 107827806 A CN107827806 A CN 107827806A
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- thrombocytin
- banana skin
- extracted
- filtrate
- extraction solvent
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
- C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
- C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
- C07D209/16—Tryptamines
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- Organic Chemistry (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of method that thrombocytin is extracted from banana skin, it is related to the extractive technique fields of natural materials.The method that thrombocytin is extracted from banana skin of the present invention includes：Fresh banana skin is taken, adds water to crush slurrying and obtains slurries；It is 1 according to the ratio between volume of slurries and Extraction solvent:35 add Extraction solvent into slurries, and Extraction solvent is mixed by first alcohol and water, and is extracted under ultrasonic wave added；Filter to take the first filtrate and the first filter residue；Solution of trichloroacetic acid is added into the first filtrate, is staticly settled, filters to take the second filtrate；Absolute ethyl alcohol is added into the second filtrate, is staticly settled, filters to take precipitation；Precipitation is washed with absolute ethyl alcohol and ether respectively to be produced.The simple and easy cost of method of the present invention is low, and it uses double solvents to banana skin extract while using ultrasound as auxiliary, and the extraction efficiency of thrombocytin is high, provides a kind of new approach for the recycling of banana skin, and provide basis for the extensive use of thrombocytin.
Description
Technical field
The present invention relates to natural materials extractive technique field, especially a kind of method that thrombocytin is extracted from banana skin.
Background technology
Banana is one of four big fruit in the world, be the maximum fruit of volume of world trade, and global consumption figure is most
Fruit.In China, banana is to occupy the fourth-largest fruit after apple, grape and citrus.Banana is due to intolerant to accumulating, often resulting in
Heavy losses after adopting, therefore some banana main production countries produce banana food to reduce damage by source establishing deep processing enterprise
Lose, at the same time produce the banana skin accessory substance of intimate fruit weight 35% or so.Such as abandoned as discarded object in field,
Not only waste of resource but also environment had been polluted, while can have also propagated some latency germs, be negatively affected to production.Therefore, develop
Using the food byproducts such as banana skin and discarded object, can protecting ecology can improve the economic benefits of food industries again, contain huge
Big market potential.
Banana skin is rich in tryptophan, vitamin B6 and abundant thrombocytin, can produce it is comfortable, meet, be happy, satisfied,
With the mood of optimism.Wherein, the circulation of thrombocytin and sleep quality and awakening is closely connected, when regaining consciousness and being movable, in brain
Serotonin level highest, but enter deep sleep stages when there's almost no, during sleep, the melatonin levels of human body
It is increased sharply, the generation of epiphysin is to provide energy by thrombocytin depending on its pineal body synthesis.In addition, serum
Plain or a kind of neurotransmitter, nerve cell need to be by thrombocytin transmission informations；Thrombocytin can alleviate pressure and irritable mood.
But do not have the ripe method that thrombocytin is extracted from banana skin in the prior art, limit its development and application.
The content of the invention
The goal of the invention of the present invention is：For above-mentioned problem, there is provided one kind extracts thrombocytin from banana skin
Method, the simple and easy cost of this method is low, and thrombocytin can be expeditiously extracted from banana skin, promotes banana skin
Recycle, basis is provided for the extensive use of thrombocytin.
The technical solution adopted by the present invention is as follows：
A kind of method that thrombocytin is extracted from banana skin, it comprises the following steps：
Step 1：Fresh banana skin is taken, adds water to crush slurrying and obtains slurries；
Step 2：It is 1 according to the ratio between volume of slurries and Extraction solvent:3-5 adds Extraction solvent into slurries, Extraction solvent by
First alcohol and water mixes, and is extracted under ultrasonic wave added；Filter to take the first filtrate and the first filter residue；
Step 3：Solution of trichloroacetic acid is added into the first filtrate, is staticly settled, filters to take the second filtrate；
Step 4：Absolute ethyl alcohol is added into the second filtrate, is staticly settled, filters to take precipitation；
Step 5：Precipitation is washed with absolute ethyl alcohol and ether respectively, is produced.
By adopting the above-described technical solution, thrombocytin solubility in the mixed solution of first alcohol and water is high, while with super
Sound efficiently can extract thrombocytin as auxiliary from banana skin.Trichloroacetic acid is molten to sink soluble protein
Shallow lake comes out.Thrombocytin dissolubility in the ethanol of high concentration is low, is thus precipitated out.
A kind of method that thrombocytin is extracted from banana skin of the present invention, in step 1, the quality of fresh banana skin and water
The ratio between be 1:1-2.
By adopting the above-described technical solution, by adopting the above-described technical solution, on the one hand it is easy to banana skin slurrying, separately
On the one hand, it is easy to the dissolving of water soluble materials.
A kind of method that thrombocytin is extracted from banana skin of the present invention, in step 2, Extraction solvent is pressed by methanol and water
It is 5-9 according to volume ratio:3 ratio mixes.
A kind of method that thrombocytin is extracted from banana skin of the present invention, adds acetic acid in the Extraction solvent, regulation carries
Take the pH to 4-4.4 of solvent.
By adopting the above-described technical solution, the solubility highest in Extraction solvent of the thrombocytin under the pH, improves it
Extraction efficiency.
A kind of method that thrombocytin is extracted from banana skin of the present invention, ultrasonic power 200-250w, frequency 22-
25KHz。
By adopting the above-described technical solution, the ultrasound condition can destroy the tissue of banana skin, the dissolution rate of material is improved,
But will not saboteur's structure.
A kind of method that thrombocytin is extracted from banana skin of the present invention, extraction time 6-8h.
A kind of method that thrombocytin is extracted from banana skin of the present invention, in step 3, is added before ammonium sulfate, first
First filtrate is subjected to concentrated by rotary evaporation and obtains concentrate to the 1/4-1/3 of the first filtrate, during revolving, bath temperature is 40-50 DEG C.
By adopting the above-described technical solution, on the one hand vaporing away partial solvent, thrombocytin is improved in Extraction solvent
Saturation degree solubility, it is easy to its precipitation, on the other hand can reduces the dosage of trichloroacetic acid and absolute ethyl alcohol, it is cost-effective.
The present invention it is a kind of from banana skin extract thrombocytin method, into the first filtrate add trichloroacetic acid to its
Percentage by volume in first filtrate is 24-28%.
By adopting the above-described technical solution, eduction rate is high in solution of trichloroacetic acid of the protein under the concentration, it is easy to
The complete precipitation of protein.
A kind of method that thrombocytin is extracted from banana skin of the present invention, in step 3, ethanol is added into the second filtrate
Percentage by volume to ethanol is 40-50%.
By adopting the above-described technical solution, solubility is relatively low in ethanol of the thrombocytin under the concentration, it is easy to thrombocytin
Complete precipitation, so as to isolate thrombocytin.
A kind of method that thrombocytin is extracted from banana skin of the present invention, into after ethanol, is placed on 0-4 DEG C of environment
Under.
By adopting the above-described technical solution, reducing temperature, the solubility of thrombocytin in the solution reduces, and separates out completeer
Entirely.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. using the mixed solution of first alcohol and water as Extraction solvent, and being aided with ultrasonic extraction, extraction efficiency is high.
2. adjusting the pH of Extraction solvent to acidity, be advantageous to the dissolving of thrombocytin, further improve extraction efficiency.
3. in extraction process, protein is removed using trichloroacetic acid precipitation, and with the ethanol precipitation thrombocytin of certain concentration,
The thrombocytin impurity content of extraction is low.
4. having effectively facilitated the recycling of banana skin, and provided the foundation for the commercial application of thrombocytin.
Embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.
This specification（Including any accessory claim, summary）Disclosed in any feature, unless specifically stated otherwise,
Replaced by other equivalent or with similar purpose alternative features.I.e., unless specifically stated otherwise, each feature is a series of
An example in equivalent or similar characteristics.
Embodiment 1
The present embodiment provides a kind of method that thrombocytin is extracted from banana skin, and this method is using compound collecting solvent to banana skin
Extracted and be aided with ultrasonic extraction, expeditiously extract thrombocytin, a kind of new approach is provided for the recycling of banana skin,
And provide basis for the extensive use of thrombocytin.The method that thrombocytin is extracted from banana skin that the present embodiment provides includes as follows
Step：
Step 1：Fresh banana skin is taken, is 1 according to the mass ratio of banana skin and water:1 adds broken slurrying after clear water, obtains
To slurries.
Step 2：Extraction solvent is accessed into slurries, Extraction solvent is 5 according to volume ratio by first alcohol and water:3 mixing and
Into the volume ratio between slurries and Extraction solvent is 1:3, it is 200w in power, frequency is 25KHz ultrasound after stirring
Under the conditions of extract 6h.After the completion of extraction, the first filter residue and the first filtrate are filtrated to get.
Step 3：It is 24% that three filter acetic acid to its percentage by volume in the first filtrate is added into the first filtrate, stirring
Uniformly, 2h is staticly settled, filters to take the second filtrate.
Step 4：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 40%, is stirred, and it is heavy to stand
Shallow lake 2h, filter to take precipitation.
Step 5：Precipitation is successively washed twice respectively with absolute ethyl alcohol and ether, is then dried in vacuo and is produced.
Embodiment 2
The present embodiment provides a kind of method that thrombocytin is extracted from banana skin, and this method is using compound collecting solvent to banana skin
Extracted and be aided with ultrasonic extraction, expeditiously extract thrombocytin, a kind of new approach is provided for the recycling of banana skin,
And provide basis for the extensive use of thrombocytin.The method that thrombocytin is extracted from banana skin that the present embodiment provides includes as follows
Step：
Step 1：Fresh banana skin is taken, is 1 according to the mass ratio of banana skin and water:2 add broken slurrying after clear water, obtain
To slurries.
Step 2：Extraction solvent is accessed into slurries, Extraction solvent is 7 according to volume ratio by first alcohol and water:3 mixing and
Into the volume ratio between slurries and Extraction solvent is 1:4, it is 220w in power, frequency is 24KHz ultrasound after stirring
Under the conditions of extract 7h.After the completion of extraction, the first filter residue and the first filtrate are filtrated to get.
Step 3：Under the conditions of 40 DEG C of bath temperature, the first filtrate is concentrated into the 1/4 of original volume, obtains concentrate, to
It is 26% that three filter acetic acid to its percentage by volume in concentrate is added in concentrate, stirs, staticly settles 2h, is filtered
Take the second filtrate.
Step 4：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 45%, is stirred, and it is heavy to stand
Shallow lake 2h, filter to take precipitation.
Step 5：Precipitation is successively washed twice respectively with absolute ethyl alcohol and ether, is then dried in vacuo and is produced.
Embodiment 3
The present embodiment provides a kind of method that thrombocytin is extracted from banana skin, and this method is using compound collecting solvent to banana skin
Extracted and be aided with ultrasonic extraction, expeditiously extract thrombocytin, a kind of new approach is provided for the recycling of banana skin,
And provide basis for the extensive use of thrombocytin.The method that thrombocytin is extracted from banana skin that the present embodiment provides includes as follows
Step：
Step 1：Fresh banana skin is taken, is 1 according to the mass ratio of banana skin and water:1 adds broken slurrying after clear water, obtains
To slurries.
Step 2：Extraction solvent is accessed into slurries, Extraction solvent is 9 according to volume ratio by first alcohol and water:3 mixing and
Into, and Extraction solvent pH to 4.0 is adjusted with acetic acid, the volume ratio between slurries and Extraction solvent is 1:5, after stirring,
Power is 250w, and frequency is to extract 8h under 22KHz ultrasound condition.After the completion of extraction, the first filter residue and the first filter are filtrated to get
Liquid.
Step 3：Under the conditions of 50 DEG C of bath temperature, the first filtrate is concentrated into the 1/3 of original volume, obtains concentrate, to
It is 28% that three filter acetic acid to its percentage by volume in concentrate is added in concentrate, stirs, staticly settles 2h, is filtered
Take the second filtrate.
Step 4：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 50%, is stirred, at 0 DEG C
Under the conditions of staticly settle 2h, filter to take precipitation.
Step 5：Precipitation is successively washed twice respectively with absolute ethyl alcohol and ether, is then dried in vacuo and is produced.
Embodiment 4
The present embodiment provides a kind of method that thrombocytin is extracted from banana skin, and this method is using compound collecting solvent to banana skin
Extracted and be aided with ultrasonic extraction, expeditiously extract thrombocytin, a kind of new approach is provided for the recycling of banana skin,
And provide basis for the extensive use of thrombocytin.The method that thrombocytin is extracted from banana skin that the present embodiment provides includes as follows
Step：
Step 1：Fresh banana skin is taken, is 1 according to the mass ratio of banana skin and water:1 adds broken slurrying after clear water, obtains
To slurries.
Step 2：Extraction solvent is accessed into slurries, Extraction solvent is 7 according to volume ratio by first alcohol and water:3 mixing and
Into, and Extraction solvent pH to 4.4 is adjusted with acetic acid, the volume ratio between slurries and Extraction solvent is 1:4, after stirring,
Power is 220w, and frequency is to extract 8h under 22KHz ultrasound condition.After the completion of extraction, the first filter residue and the first filter are filtrated to get
Liquid.
Step 3：Under the conditions of 45 DEG C of bath temperature, the first filtrate is concentrated into the 1/4 of original volume, obtains concentrate, to
It is 26% that three filter acetic acid to its percentage by volume in concentrate is added in concentrate, stirs, staticly settles 2h, is filtered
Take the second filtrate.
Step 4：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 48%, is stirred, at 4 DEG C
Under the conditions of staticly settle 2h, filter to take precipitation.
Step 5：Precipitation is successively washed twice respectively with absolute ethyl alcohol and ether, is then dried in vacuo and is produced.
The invention is not limited in foregoing embodiment.The present invention, which expands to, any in this manual to be disclosed
New feature or any new combination, and disclose any new method or process the step of or any new combination.
Claims (10)
- A kind of 1. method that thrombocytin is extracted from banana skin, it is characterised in that it comprises the following steps：Step 1：Fresh banana skin is taken, adds water to crush slurrying and obtains slurries；Step 2：It is 1 according to the ratio between volume of slurries and Extraction solvent:3-5 adds Extraction solvent into slurries, Extraction solvent by First alcohol and water mixes, and is extracted under ultrasonic wave added；Filter to take the first filtrate and the first filter residue；Step 3：Solution of trichloroacetic acid is added into the first filtrate, is staticly settled, filters to take the second filtrate；Step 4：Absolute ethyl alcohol is added into the second filtrate, is staticly settled, filters to take precipitation；Step 5：Precipitation is washed with absolute ethyl alcohol and ether respectively, is produced.
- 2. the method according to claim 1 that thrombocytin is extracted from banana skin, it is characterised in that fresh in step 1 The mass ratio of banana skin and water is 1:1-2.
- 3. the method according to claim 1 that thrombocytin is extracted from banana skin, it is characterised in that in step 2, extraction Solvent is 5-9 according to volume ratio by methanol and water:3 ratio mixes.
- 4. the method that thrombocytin is extracted from banana skin according to right will go 3, it is characterised in that in the Extraction solvent Acetic acid is added, adjusts the pH to 4-4.4 of Extraction solvent.
- 5. according to the method that thrombocytin is extracted from banana skin described in right 4, it is characterised in that ultrasonic power 200- 250w, frequency 22-25KHz.
- 6. the method according to claim 5 that thrombocytin is extracted from banana skin, it is characterised in that extraction time 6- 8h。
- 7. the method according to claim 1 that thrombocytin is extracted from banana skin, it is characterised in that in step 3, add Before ammonium sulfate, the first filtrate is first subjected to concentrated by rotary evaporation and obtains concentrate to the 1/4-1/3 of the first filtrate, during revolving, Bath temperature is 40-50 DEG C.
- 8. the method according to claim 6 that thrombocytin is extracted from banana skin, it is characterised in that add into the first filtrate It is 24-28% to enter trichloroacetic acid to its percentage by volume in the first filtrate.
- 9. the method according to claim 1 that thrombocytin is extracted from banana skin, it is characterised in that in step 3, to the The percentage by volume that ethanol to ethanol are added in two filtrates is 40-50%.
- 10. the method according to claim 9 that thrombocytin is extracted from banana skin, it is characterised in that, will into after ethanol It is placed under 0-4 DEG C of environment.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711233264.1A CN107827806A (en) | 2017-11-30 | 2017-11-30 | A kind of method that thrombocytin is extracted from banana skin |
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| CN201711233264.1A CN107827806A (en) | 2017-11-30 | 2017-11-30 | A kind of method that thrombocytin is extracted from banana skin |
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| CN107827806A true CN107827806A (en) | 2018-03-23 |
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| CN201711233264.1A Withdrawn CN107827806A (en) | 2017-11-30 | 2017-11-30 | A kind of method that thrombocytin is extracted from banana skin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110483362A (en) * | 2019-09-11 | 2019-11-22 | 广东石油化工学院 | The process of serotonin is extracted in a kind of response phase method optimization from banana |
-
2017
- 2017-11-30 CN CN201711233264.1A patent/CN107827806A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110483362A (en) * | 2019-09-11 | 2019-11-22 | 广东石油化工学院 | The process of serotonin is extracted in a kind of response phase method optimization from banana |
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