CN107789448A - A kind of enzymatic extraction method of plant skin element - Google Patents
A kind of enzymatic extraction method of plant skin element Download PDFInfo
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- CN107789448A CN107789448A CN201711233083.9A CN201711233083A CN107789448A CN 107789448 A CN107789448 A CN 107789448A CN 201711233083 A CN201711233083 A CN 201711233083A CN 107789448 A CN107789448 A CN 107789448A
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- extraction method
- skin element
- filtrate
- enzymatic extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of enzymatic extraction method of plant skin element, it is related to the extractive technique field of natural materials.The enzymatic extraction method of the plant skin element of the present invention includes：Dry banana skin adds to be scattered in drinking water after crushing obtains dispersion liquid；Complex enzyme is added into dispersion liquid to be digested to obtain enzymolysis liquid；Enzymolysis liquid is filtered to take into the first filtrate；Ammonium sulfate is added into the first filtrate, is staticly settled, filters to take the second filtrate；Absolute ethyl alcohol is added into the second filtrate, is staticly settled, filters to take precipitation；Precipitation produces after absolute ethyl alcohol and ether wash respectively.The enzymatic extraction method of plant skin element provided by the invention is simple and easy, carries out enzymolysis and extraction to banana skin using complex enzyme, expeditiously extracts plant skin element, provide a kind of new approach for the recycling of banana skin, and provide basis for the extensive use of plant skin element.
Description
Technical field
The present invention relates to natural materials extractive technique field, especially a kind of enzymatic extraction method of plant skin element.
Background technology
Banana is one of four big fruit in the world, be the maximum fruit of volume of world trade, and global consumption figure is most
Fruit.In China, banana is to occupy the fourth-largest fruit after apple, grape and citrus.Banana is due to intolerant to accumulating, often resulting in
Heavy losses after adopting, therefore some banana main production countries produce banana food to reduce damage by source establishing deep processing enterprise
Lose, at the same time produce the banana skin accessory substance of intimate fruit weight 35% or so.Such as abandoned as discarded object in field,
Not only waste of resource but also environment had been polluted, while can have also propagated some latency germs, be negatively affected to production.Therefore, develop
Using the food byproducts such as banana skin and discarded object, can protecting ecology can improve the economic benefits of food industries again, contain huge
Big market potential.
Contain abundant nutriment, such as protein, fat, sugar, mineral matter and trace element in banana.Wherein,
Plant skin element can effectively suppress cutaneous pruritus caused by fungi and bacterial growth breeding, treatment tinea pedis, the tinea manuum, ringworm of the body etc., and
Phenomena such as skin aging, decortication, itch, chap can be resisted.But do not have being extracted from banana skin for maturation in the prior art
The method of plant skin element, limit its development and application.
The content of the invention
The goal of the invention of the present invention is：For above-mentioned problem, there is provided a kind of enzymatic extraction method of plant skin element,
Each nutrient is dissolved out by this method by way of enzymolysis from banana skin, finally gives plant skin element, promotes banana
The recycling of skin, basis is provided for the extensive use of plant skin element, meanwhile, the simple and easy cost of this method is low.
The technical solution adopted by the present invention is as follows：
A kind of enzymatic extraction method of plant skin element, it is characterised in that it comprises the following steps：
Step 1：Dry banana skin adds to be scattered in drinking water after crushing obtains dispersion liquid；
Step 2：Complex enzyme is added into dispersion liquid to be digested to obtain enzymolysis liquid；Enzymolysis liquid is filtered to take into the first filtrate；
Step 3：Ammonium sulfate is added into the first filtrate, is staticly settled, filters to take the second filtrate；
Step 4：Absolute ethyl alcohol is added into the second filtrate, is staticly settled, filters to take precipitation；
Step 5：Precipitation produces after absolute ethyl alcohol and ether wash respectively.
By adopting the above-described technical solution, complex enzyme enzymolytic tissue cell first, is by the nutriment in banana skin
Release, then digest macromolecular substances into small molecule, plant skin element separate out, then by ammonium sulfate precipitation, separate out non-enzyme
The protein and/or amino acid of solution, are then precipitated plant skin element with the ethanol of high concentration.This method is simple and easy, to plant skin element
Extraction efficiency it is high, also, the plant skin element impurity content that extraction obtains is low.
A kind of enzymatic extraction method of plant skin element of the present invention, in step 1, dry banana skin crosses 40 mesh sieves after crushing.
By adopting the above-described technical solution, it is easy to the enzymolysis of complex enzyme.
A kind of enzymatic extraction method of plant skin element of the present invention, it is 1 according to banana skin and the solid-liquid ratio of reference:4-6（kg：
L）Ratio add drinking water.
A kind of enzymatic extraction method of plant skin element of the present invention, in step 2, complex enzyme by lipase, papain,
Phytase cellulase and hemicellulase are 1 according to mass ratio:0.4-0.6:0.3-0.4:0.1-0.2:0.1-0.2 groups
Into.
A kind of enzymatic extraction method of plant skin element of the present invention, mass concentration of the complex enzyme in dispersion liquid is 1-
10‰。
By adopting the above-described technical solution, cellulase and hemicellulase lysed cells wall, destroy histocyte.Fat
Fat enzyme is used to digest fat, papain and phytase collective effect, for complete enzymolysis protein, by fat, protein etc.
After macromolecular and the molecule enzymolysis easily chelated, plant skin element is easier to be released.
A kind of enzymatic extraction method of plant skin element of the present invention, enzymolysis time continue 4-8h, and enzymolysis process temperature maintains 35-
45 DEG C, after the completion of enzymolysis, 80-100 DEG C is warming up to by enzyme-deactivating.
By adopting the above-described technical solution, enzymolysis is complete.
The enzymatic extraction method of a kind of plant skin element of the present invention, before complex enzyme is added into dispersion liquid, with 40-50 DEG C of temperature
Moisture dissipates, and is incubated 10-30min.
By adopting the above-described technical solution, before use, complex enzyme is activated, beneficial to the quick progress of enzymolysis.
A kind of enzymatic extraction method of plant skin element of the present invention, in step 3, add before ammonium sulfate, first by first
Filtrate carries out concentrated by rotary evaporation to the 1/4-1/3 of the first filtrate, and during revolving, bath temperature is 50-70 DEG C.
By adopting the above-described technical solution, by adopting the above-described technical solution, on the one hand vapor away part low boiling
Organic solvent, reduce solubility of the plant skin element in Extraction solvent, be easy to its precipitation, it is molten on the other hand can to reduce ammonium sulfate
The dosage of liquid and absolute ethyl alcohol, it is cost-effective.
A kind of enzymatic extraction method of plant skin element of the present invention, it is 1 according to the volume ratio of concentrate and ammonium sulfate:1-
2 ratio adds the ammonium sulfate that mass percent is 46%-70% into concentrate.
By adopting the above-described technical solution, eduction rate is high in the ammonium sulfate of protein and amino acid under the concentration,
It is easy to the complete precipitation of the two.
A kind of enzymatic extraction method of plant skin element of the present invention, in step 4, absolute ethyl alcohol is added extremely into the second filtrate
Untill the percentage by volume of ethanol is 70-90%.
By adopting the above-described technical solution, solubility is told somebody what one's real intentions are in ethanol of the plant skin element under the concentration, it is easy to plant skin element
Complete precipitation, so as to isolate plant skin element.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. extracting plant skin element in the method for enzymolysis, extraction efficiency is high.
2. in extraction process, protein is removed using ammonium sulfate precipitation, and with the ethanol precipitation plant skin element of certain concentration, is carried
The plant skin element impurity content taken is low.
3. having effectively facilitated the recycling of banana skin, and provided the foundation for the commercial application of plant skin element.
Embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.
This specification（Including any accessory claim, summary）Disclosed in any feature, unless specifically stated otherwise,
Replaced by other equivalent or with similar purpose alternative features.I.e., unless specifically stated otherwise, each feature is a series of
An example in equivalent or similar characteristics.
Embodiment 1
The present embodiment provides a kind of enzymatic extraction method of plant skin element, and this method carries out enzymolysis to banana skin using complex enzyme and carried
Take, expeditiously extract plant skin element, provide a kind of new approach for the recycling of banana skin, and be the extensive use of plant skin element
Basis is provided.The enzymatic extraction method for the plant skin element that the present embodiment provides comprises the following steps：
Step 1：Dry banana skin is taken, 40 mesh sieves are crossed after crushing, by the banana skin through crushing and clear water according to mass volume ratio
For 1:4（kg：L）Ratio be scattered in clear water and obtain dispersion liquid.
Step 2：Add complex enzyme into dispersion liquid to be digested, complex enzyme is 1 by mass ratio:0.4:0.4:0.1:0.1
Lipase, papain, phytase cellulase and hemicellulase composition, the complex enzyme of addition is in dispersion liquid
Mass concentration is 1 ‰, and keeping temperature is 35 DEG C, digests 8h, then heats to 80 DEG C and terminates enzymolysis, obtains enzymolysis liquid.Will enzymolysis
Liquid filters, and obtains the first filtrate.
Step 3：It is 1 according to the volume ratio of ammonium sulfate and the first filtrate:1 adds mass fraction into the first filtrate
For 70% ammonium sulfate, after stirring, 2h is staticly settled, filters to take the second filtrate.
Step 4：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 70%, is stirred, and it is heavy to stand
Shallow lake 2h, filter to take precipitation.
Step 5：Precipitation is successively washed twice respectively with absolute ethyl alcohol and ether, is then drying to obtain at 60 DEG C.
Embodiment 2
The present embodiment provides a kind of enzymatic extraction method of plant skin element, and this method carries out enzymolysis to banana skin using complex enzyme and carried
Take, expeditiously extract plant skin element, provide a kind of new approach for the recycling of banana skin, and be the extensive use of plant skin element
Basis is provided.The enzymatic extraction method for the plant skin element that the present embodiment provides comprises the following steps：
Step 1：Dry banana skin is taken, 40 mesh sieves are crossed after crushing, by the banana skin through crushing and clear water according to mass volume ratio
For 1:5（kg：L）Ratio be scattered in clear water and obtain dispersion liquid.
Step 2：Add complex enzyme into dispersion liquid to be digested, complex enzyme is 1 by mass ratio:0.5:0.3:0.1:0.1
Lipase, papain, phytase cellulase and hemicellulase composition, the complex enzyme of addition is in dispersion liquid
Mass concentration is 4 ‰, and complex enzyme is added before dispersion liquid, and the warm water with 40 DEG C disperses, and is incubated 30min.Keeping temperature is 40
DEG C, 6h is digested, 90 DEG C is then heated to and terminates enzymolysis, obtain enzymolysis liquid.Enzymolysis liquid is filtered, obtains the first filtrate.
Step 3：Under conditions of bath temperature is 50 DEG C, by the first filtrate concentrated by rotary evaporation to the 1/4 of original volume, obtain
Concentrate.It is 1 according to the volume ratio of ammonium sulfate and concentrate:2 add the ammonium sulfate that mass fraction is 46% into concentrate
Solution, after stirring, 2h is staticly settled, filter to take the second filtrate.
Step 4：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 80%, is stirred, and it is heavy to stand
Shallow lake 2h, filter to take precipitation.
Step 5：Precipitation is successively washed twice respectively with absolute ethyl alcohol and ether, is then drying to obtain at 60 DEG C.
Embodiment 3
The present embodiment provides a kind of enzymatic extraction method of plant skin element, and this method carries out enzymolysis to banana skin using complex enzyme and carried
Take, expeditiously extract plant skin element, provide a kind of new approach for the recycling of banana skin, and be the extensive use of plant skin element
Basis is provided.The enzymatic extraction method for the plant skin element that the present embodiment provides comprises the following steps：
Step 1：Dry banana skin is taken, 40 mesh sieves are crossed after crushing, by the banana skin through crushing and clear water according to mass volume ratio
For 1:6（kg：L）Ratio be scattered in clear water and obtain dispersion liquid.
Step 2：Add complex enzyme into dispersion liquid to be digested, complex enzyme is 1 by mass ratio:0.6:0.3:0.2:0.2
Lipase, papain, phytase cellulase and hemicellulase composition, the complex enzyme of addition is in dispersion liquid
Mass concentration is 8 ‰, and complex enzyme is added before dispersion liquid, and the warm water with 50 DEG C disperses, and is incubated 10min.Keeping temperature is 45
DEG C, 4h is digested, 100 DEG C is then heated to and terminates enzymolysis, obtain enzymolysis liquid.Enzymolysis liquid is filtered, obtains the first filtrate.
Step 3：Under conditions of bath temperature is 60 DEG C, by the first filtrate concentrated by rotary evaporation to the 1/3 of original volume, obtain
Concentrate.It is 1 according to the volume ratio of ammonium sulfate and concentrate:1 adds the ammonium sulfate that mass fraction is 68% into concentrate
Solution, after stirring, 2h is staticly settled, filter to take the second filtrate.
Step 4：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 90%, is stirred, and it is heavy to stand
Shallow lake 2h, filter to take precipitation.
Embodiment 4
The present embodiment provides a kind of enzymatic extraction method of plant skin element, and this method carries out enzymolysis to banana skin using complex enzyme and carried
Take, expeditiously extract plant skin element, provide a kind of new approach for the recycling of banana skin, and be the extensive use of plant skin element
Basis is provided.The enzymatic extraction method for the plant skin element that the present embodiment provides comprises the following steps：
Step 1：Dry banana skin is taken, 40 mesh sieves are crossed after crushing, by the banana skin through crushing and clear water according to mass volume ratio
For 1:5（kg：L）Ratio be scattered in clear water and obtain dispersion liquid.
Step 2：Add complex enzyme into dispersion liquid to be digested, complex enzyme is 1 by mass ratio:0.5:0.3:0.1:0.2
Lipase, papain, phytase cellulase and hemicellulase composition, the complex enzyme of addition is in dispersion liquid
Mass concentration is 10 ‰, and complex enzyme is added before dispersion liquid, and the warm water with 45 DEG C disperses, and is incubated 20min.Keeping temperature is 37
DEG C, 6h is digested, 100 DEG C is then heated to and terminates enzymolysis, obtain enzymolysis liquid.Enzymolysis liquid is filtered, obtains the first filtrate.
Step 3：Under conditions of bath temperature is 60 DEG C, by the first filtrate concentrated by rotary evaporation to the 1/4 of original volume, obtain
Concentrate.It is 1 according to the volume ratio of ammonium sulfate and concentrate:2 add the ammonium sulfate that mass fraction is 54% into concentrate
Solution, after stirring, 2h is staticly settled, filter to take the second filtrate.
Step 4：Absolute ethyl alcohol is added into the second filtrate, the volume fraction to ethanol is 80%, is stirred, and it is heavy to stand
Shallow lake 2h, filter to take precipitation.
The invention is not limited in foregoing embodiment.The present invention, which expands to, any in this manual to be disclosed
New feature or any new combination, and disclose any new method or process the step of or any new combination.
Claims (10)
1. a kind of enzymatic extraction method of plant skin element, it is characterised in that it comprises the following steps：
Step 1：Dry banana skin adds to be scattered in drinking water after crushing obtains dispersion liquid；
Step 2：Complex enzyme is added into dispersion liquid to be digested to obtain enzymolysis liquid；Enzymolysis liquid is filtered to take into the first filtrate；
Step 3：Ammonium sulfate is added into the first filtrate, is staticly settled, filters to take the second filtrate；
Step 4：Absolute ethyl alcohol is added into the second filtrate, is staticly settled, filters to take precipitation；
Step 5：Precipitation produces after absolute ethyl alcohol and ether wash respectively.
2. the enzymatic extraction method of plant skin element according to claim 1, it is characterised in that in step 1, dry banana
Skin crosses 40 mesh sieves after crushing.
3. the enzymatic extraction method of according to claim 2 plus skin element, it is characterised in that according to banana skin and drinking water
Solid-liquid ratio is 1:4-6（kg：L）Ratio add drinking water.
4. the enzymatic extraction method of plant skin element according to claim 1, it is characterised in that in step 2, complex enzyme is by fat
Fat enzyme, papain, phytase cellulase and hemicellulase are 1 according to mass ratio:0.4-0.6:0.3-0.4:
0.1-0.2:0.1-0.2 is formed.
5. the enzymatic extraction method of plant skin element according to claim 4, it is characterised in that the complex enzyme is in dispersion liquid
Mass concentration be 1-10 ‰.
6. the enzymatic extraction method of plant skin element according to claim 5, it is characterised in that enzymolysis time continues 4-8h, enzyme
Solution preocess temperature maintains 35-45 DEG C, after the completion of enzymolysis, is warming up to 80-100 DEG C by enzyme-deactivating.
7. the enzymatic extraction method of plant skin element according to claim 6, it is characterised in that by complex enzyme add dispersion liquid it
Before, disperseed with 40-50 DEG C of warm water, and be incubated 10-30min.
8. the enzymatic extraction method of plant skin element according to claim 1, it is characterised in that in step 3, add ammonium sulfate
Before solution, the first filtrate is first subjected to concentrated by rotary evaporation and obtains concentrate to the 1/4-1/3 of the first filtrate, during revolving, water-bath temperature
Spend for 50-70 DEG C.
9. the enzymatic extraction method of plant skin element according to claim 8, it is characterised in that molten with ammonium sulfate according to concentrate
The volume ratio of liquid is 1:1-2 ratio adds the ammonium sulfate that mass percent is 46%-70% into concentrate.
10. the enzymatic extraction method of plant skin element according to claim 1, it is characterised in that in step 4, to the second filtrate
The middle absolute ethyl alcohol that adds is untill the percentage by volume of ethanol is 70-90%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711233083.9A CN107789448A (en) | 2017-11-30 | 2017-11-30 | A kind of enzymatic extraction method of plant skin element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711233083.9A CN107789448A (en) | 2017-11-30 | 2017-11-30 | A kind of enzymatic extraction method of plant skin element |
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| Publication Number | Publication Date |
|---|---|
| CN107789448A true CN107789448A (en) | 2018-03-13 |
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ID=61537291
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711233083.9A Withdrawn CN107789448A (en) | 2017-11-30 | 2017-11-30 | A kind of enzymatic extraction method of plant skin element |
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| CN (1) | CN107789448A (en) |
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2017
- 2017-11-30 CN CN201711233083.9A patent/CN107789448A/en not_active Withdrawn
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Application publication date: 20180313 |