CN107929225A - A kind of radiation protection containing sweet osmanthus stem cell extract, acne-removing composition and preparation method thereof - Google Patents
A kind of radiation protection containing sweet osmanthus stem cell extract, acne-removing composition and preparation method thereof Download PDFInfo
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- CN107929225A CN107929225A CN201711336353.9A CN201711336353A CN107929225A CN 107929225 A CN107929225 A CN 107929225A CN 201711336353 A CN201711336353 A CN 201711336353A CN 107929225 A CN107929225 A CN 107929225A
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- acne
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- Microbiology (AREA)
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- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides a kind of radiation protection containing sweet osmanthus stem cell extract, acne-removing composition, include the component of following parts by weight:5 10 parts of sweet osmanthus stem cell extract, 68 parts of buckbean leaf extract, 35 parts of asparagus extract.Said composition not only has preferable anti-acne effect, and said composition also has preferable radiation-proof effect, and safe and non-stimulating.
Description
Technical field
The present invention relates to a kind of radiation protection containing sweet osmanthus stem cell extract, acne-removing composition with and preparation method thereof.
Background technology
For neutral and oiliness acne skin, the secretion of its skin oil and fat is vigorous, and grease heap is easily caused at forehead, the wing of nose
Product, and more pores are thick, and skin is thick hard rough.The grease excessively secreted be easy to cause the blocking of pore, blackhead and
The components such as hormone, antibiotic, vitamin A acid, tartaric acid, irritation are used in the growth of small pox, at present, many anti-acne (acne of dispelling) products
Greatly, drug resistance of the skin to Propiobacterium is easily caused, security is constantly under suspicion.For example, acne are often used as,
Itch-stopping bactericidal, the dosage that the salicylic acid of softening skin cuticula cannot be more than 0.5%, and had dry skin after, it can produce
Tight feeling, and in existing natural composition product for resolving poxes, often simply single selection natural extract carrys out anti-inflammation and sterilization, not
There is the bacterium for caused acne to solve the problems, such as, so the speed that takes effect is frequently not quickly.
Ionising radiation (such as x-ray, neutron, proton, α or β particles, gamma-rays) can be directly or by secondary reaction infringement group
Knit.High dose radiation can produce visible body effect within a couple of days.DNA changes caused by low dose can produce illuminated person
Chronic disease, makes relation between the healing or death of their offspring's development genetics defect degree of injury and cell very
It is complicated.Harmful ionized radiation source is including being used for high-energy X-ray, radium and other naturally occurring radioactive substances of diagnose and treat (such as
Radon), nuclear reactor, cyclotron, linear accelerator, variable gradient synchrotron, the cobalt of the sealing for treating cancerous swelling
With caesium and be largely used to medicine and industry artificially generated radioactive substance.After sufficiently high dose irradiation, it can occur
Meronecrosis.High sublethal dose can slow down DNA synthesis or cell is become polypoid by reducing mitosis rate, and
The propagation of interference cell.It can be produced after being carried out continuously the tissue (such as enteric epithelium, marrow, sexual gland) of renewal via radiation dose-dependent
Progressive hypoplasia, atrophy, final fibrosis.It is some to be damaged but can still provide for mitotic cell, before its death also
Abnormal offspring (such as huge metamylocyte, the excessive neutrophil of leaflet) can be produced through 1 or 2 cyclostage.Have
According to the report, separately add the time-to-live of animal of the animal survival time than only receiving background exposure of very low dose irradiation long.It is long
People are finding radioprotectant since phase, but do not find out a preferable medicine yet so far and be used to treat and prevent radiation institute
The toxic side effect of cause.
Stem cell is a kind of seed cell of not yet differentiation and development, has unlimited self-renewing duplication and differentiation potential, this
It is the basis that stem cell is used for beauty product.Plant stem cell is one be present in shoot apical meristem and Root apical meristem
Class special cells group, has self-renewal capacity, and can produce the daughter cell with lasting splitting ability, these special cells are
The source of the generation of the organs such as plant roots, stem, leaf and flower.Plant stem cell technology is the wound recovery mechanisms using plant as base
Plinth, Schilling plant form wound and restore histiocytic formation so as to induce, and it is i.e. dry that recovery tissue contains undifferentiated cell
Cell, these recovery histocytes are collected and are cultivated in nutrient solution.Stem cell beautifying technique and its Related product are near
2 years extensive concerns for being subject to beauty circle expert and insider.
The content of the invention
It is an object of the present invention to provide a kind of radiation protection containing sweet osmanthus stem cell extract, acne-removing composition and
Its preparation method, which not only has preferable acne-removal function, but also can play the role of radiation protection.
To achieve the above object, the technical solution used in the present invention:A kind of anti-spoke containing sweet osmanthus stem cell extract
Penetrate, acne-removing composition, include the component of following parts by weight:5-10 parts of sweet osmanthus stem cell extract, 6-8 parts of buckbean leaf extract,
3-5 parts of asparagus extract.
Single extract is usually used in the prior art and carries out anti-acne or radiation protection, but its effect is not managed as far as possible
Think, present inventor has found by numerous studies, and sweet osmanthus stem cell extract, buckbean leaf extract are compounded, it is anti-
Radiation effect is significantly better than independent extract;Sweet osmanthus stem cell extract, asparagus extract are compounded, its anti-acne effect
It is significantly better than independent extract.
Preferably, the weight ratio of the sweet osmanthus stem cell extract and buckbean leaf extract is 1:1.Present inventor passes through
Numerous studies discovery is crossed, is matched herein, its radiation-proof effect is optimal.
Preferably, the weight ratio of the sweet osmanthus stem cell extract and asparagus extract is 2:1.Present inventor passes through
Numerous studies discovery is crossed, is matched herein, its anti-acne effect is optimal.
Preferably, the radiation protection, acne-removing composition further include preservative, pH adjusting agent, thickener, emulsifying agent, essence,
Pigment and water.
Preferably, the emulsifying agent is selected from ceteareth -6, ceteareth -25, glycerol monostearate
Ester, the emollient be selected from White Mineral Oil, stearic acid, cetostearyl alcohol, olive oil, octanoic acid/certain herbaceous plants with big flowers acid triglyceride, shea butter,
Jojoba oil, silicone oil, the skin moisturizer are selected from glycerine, propane diols, 1,3 butanediols.
The present invention provides radiation protection described above, the preparation method of acne-removing composition, the preparation method is will be each
Component raw material precise by weight ratio, is sufficiently mixed and stirs evenly, that is, obtains the composition.
Preferably, the radiation protection, the formulation of acne-removing composition are aqua, creme, finish, washing lotion or emulsion.
The present invention provides a kind of radiation protection, anti-acne skin care item, including oil phase, water phase, the water mutually includes described above
Radiation protection, acne-removing composition.
Preferably, the radiation protection, anti-acne skin care item further include essence and preservative.
The beneficial effects of the present invention are:The present invention provides a kind of anti-acne containing sweet osmanthus stem cell extract, net white
Composition, not only anti-acne effect is good for said composition, and said composition also has preferable radiation-proof effect, and safe and non-stimulating.
Embodiment
Each raw material involved by following embodiments is the commercially available universal product unless otherwise instructed.
The preparation method of sweet osmanthus stem cell extract of the present invention comprises the following steps:
1) isolation and culture of sweet osmanthus forming layer stem cell:By sweet osmanthus blade after disinfecting, aseptically,
Sweet osmanthus blade after disinfection is cut into 0.5cm*0.5cm sizes, is seeded on WPM culture mediums, leaf back is connect with WPM culture mediums
Touch, the dark culturing at 25 DEG C;Forming layer stem cell is transferred in subculture medium after two weeks and is cultivated, every two weeks subculture one
It is secondary, obtain sweet osmanthus forming layer stem cell;
2) culture medium after culture sweet osmanthus forming layer stem cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes culture medium
In it is phenol red, retain culture medium in active ingredient;Culture medium and the lysate of sweet osmanthus forming layer stem cell are freeze-dried, obtained
To sweet osmanthus stem cell extract.
Embodiment 1
Radiation protection of the present invention, a kind of embodiment of acne-removing composition, the composition include the group of following parts by weight
Point:8 parts of sweet osmanthus stem cell extract, 8 parts of buckbean leaf extract, 4 parts of asparagus extract.
Embodiment 2
Radiation protection of the present invention, a kind of embodiment of acne-removing composition, the composition include the group of following parts by weight
Point:5 parts of sweet osmanthus stem cell extract, 8 parts of buckbean leaf extract, 5 parts of asparagus extract.
Embodiment 3
Radiation protection of the present invention, a kind of embodiment of acne-removing composition, the composition include the group of following parts by weight
Point:10 parts of sweet osmanthus stem cell extract, 8 parts of buckbean leaf extract, 3 parts of asparagus extract.
Comparative example 1
The formula of comparative example 1 is:12 parts by weight of sweet osmanthus stem cell extract, 8 parts by weight of buckbean leaf extract.
Comparative example 2
The formula of comparative example 2 is:8 parts by weight of buckbean leaf extract., 12 parts by weight of asparagus extract.
The preparation method of embodiment 1-3 and comparative example 1,2:Each component raw material is pressed into above-mentioned weight proportion precise,
It is sufficiently mixed and stirs evenly, you can.
The composition of embodiment 1-3 and comparative example 1,2 are prepared into blemish clearing gel, by carrier and functional component two parts group
Into, by weight percentage, including following component:
Carrier:Ammonium acryloyldime-thyltaurate/vinylpyrrolidone copolymer 1.5%, glycerine 5%, 1,2- penta 2
Alcohol:3%, deionized water 86.6%;
Functional component:One kind 0.1% in embodiment 1-3 and the composition of comparative example 1,2.
Carrier component is heated to 85 DEG C, after it is completely dissolved, blender is enabled and is stirred 30min, use circulation
When cooling and stirring is cooled to 45 DEG C, stirred when adding functional component, until stirring evenly.
First, 1-3 of embodiment of the present invention acne-removings evaluation test
To 60 ages, the whelk patient at 20~28 years old tests, wherein man, each 30 of female.6 groups are divided into,
Every group of 10 people (each 5 of men and women), tests embodiment 1~3 and comparative example 1,2.Application method is:After face cleaning, cotton is used
The blemish clearing gel of above-described embodiment 1~3 and the gained of comparative example 1,2 is applied directly to affected part by label, is smeared 1~3 time daily, even
28 days continuous, observation affected part swelling, pain, itch and whether there is allergic symptom, and with treated effect, (affected part is obviously reduced, sufferings symptom
The processing acne points substantially mitigated account for the percentage of total processing acne points) to be assessed, result of the test is shown in Table 1.
1 test result of table
As shown in Table 1:Sweet osmanthus stem cell extract or asparagus extract are added in skin care item can reach anti-acne effect
Fruit, and sweet osmanthus stem cell extract and asparagus extract compounding are added in skin care item, anti-acne effect is synergistic, significantly high
In independent addition, the anti-acne effect of wherein embodiment 1 is best, even if not smearing product for resolving poxes certainly, the acne point with human body
Can a Lock-out part.
Embodiment 4
Radiation protection of the present invention, a kind of embodiment of acne-removing composition, the composition include the group of following parts by weight
Point:8 parts of sweet osmanthus stem cell extract, 8 parts of buckbean leaf extract, 4 parts of asparagus extract.
Embodiment 5
Radiation protection of the present invention, a kind of embodiment of acne-removing composition, the composition include the group of following parts by weight
Point:10 parts of sweet osmanthus stem cell extract, 6 parts of buckbean leaf extract, 4 parts of asparagus extract.
Embodiment 6
Radiation protection of the present invention, a kind of embodiment of acne-removing composition, the composition include the group of following parts by weight
Point:5 parts of sweet osmanthus stem cell extract, 8 parts of buckbean leaf extract, 4 parts of asparagus extract.
Comparative example 3
The formula of comparative example 3 is:16 parts of sweet osmanthus stem cell extract, 4 parts of asparagus extract.
Comparative example 4
The formula of comparative example 4 is:16 parts of buckbean leaf extract, 4 parts of asparagus extract.
The preparation method of embodiment 4-6 and documents 3-4:Each component raw material is accurately claimed by above-mentioned weight proportion
Amount, is sufficiently mixed and stirs evenly, you can.
4-6 anti-radiation effect evaluation tests of the embodiment of the present invention
Experimental method is as follows:
84 cleaning grade male Kunming strain mices are randomly divided into 7 groups:Blank group, model group, control group 1, control group 2,
Test group 1 (dosage 5g/kg), test group 2 (dosage 5g/kg), test group 3 (dosage 5g/kg), every group 12.Except sky
White group is outer, remaining group mouse is used60Co rays carry out a subtotal body irradiation, and irradiation total absorbed dose is 2.0Gy.Each dose of composition
Amount group mouse in60Co radiation the previous day gavage gives corresponding dosage, and blank group and model group animal gavage give isodose life
Brine is managed, each group is administered with the daily gavage of physiological saline once, continuous seven days.Each group animal is the 7th after pre-irradiation, irradiation
It adopts tail peripheral blood twice, surveys leucocyte (WBC) number.
Influence of the composition of 4-6 of the embodiment of the present invention to irradiated mice peripheral white blood cell the results are shown in Table 2.
Influence of the Chinese medicine composition of 2 4-6 of the embodiment of the present invention of table to irradiated mice peripheral white blood cell
Group | Pre-irradiation WBC (× 109) | 7th day WBC (× 10 after irradiation9) |
Blank group | 7.25±0.15 | 8.03±0.14 |
Model group | 7.73±0.15 | 3.33±014* |
Control group 1 (comparative example 3) | 7.61±0.25 | 6.10±0.25** |
Control group 2 (comparative example 4) | 7.83±0.14 | 6.11±0.13** |
Test group 1 (embodiment 4) | 7.68±0.21 | 7.18±0.12** |
Test group 2 (embodiment 5) | 7.65±0.27 | 6.62±0.25** |
Test group 3 (embodiment 6) | 7.71±0.21 | 6.65±0.21** |
* model group P < 0.01 compared with blank group;* P < 0.01 compared with model group.
As seen from the above table, model group is compared with blank group,607 days leukocyte counts significantly reduce after Co radiation exposures, have very
Significant difference (P < 0.01), that is, test modeling success.Using above-mentioned composition, murine interleukin, which counts, to be had in various degree
Rising, tool is very significant (P < 0.01) compared with model group, and the radiation-proof effect of embodiment 4 is best, adopts
It can reach radiation-proof effect with sweet osmanthus stem cell extract or buckbean leaf extract, and sweet osmanthus stem cell extract and buckbean leaf
Extract compounds, its radiation-proof effect is synergistic, is significantly higher than exclusive use.
The present composition has the function that body peripheral white blood cells caused by improving radiation injury reduce, that is, has pre-
Anti- and treatment radiation injury effect.
Present composition safety testing
Test material and method
Animal:Regular grade large ear rabbit 4,2.3-2.7 kilograms of weight, body looks into animal no abnormality seen.
Environmental condition:Room temperature:22 DEG C -23 DEG C, relative humidity:66% -68%;
Test method:
Foundation《Cosmetics health specification》(version in 2007) acute skin irritation test, before experiment 24 it is small when, will test
Back part of animal backbone diamond wool is cut, 3 centimetres of 3 cm x of unhairing scope.Tested 0.5 gram of no originality is taken directly to be applied to right side skin
On skin, with clean gauze cover, with nonirritant immobilization with adhesive tape 2 it is small when.Other side is negative control.After removing tested material
1 it is small when, 24 it is small when, 48 it is small when 72 it is small when observe and animal subject contact site dermoreaction and keep a record.
Under this experimental condition, 1-6 of embodiment of the present invention compositions press White Rabbit acute skin irritation test skin thorn
It is nonirritant to swash strength grading.
The Security test of 1-6 compositions of the embodiment of the present invention:
1-6 of embodiment of the present invention compositions safety, the examination of human body patch are demonstrated by zoopery and human body patch test
Test and carried out by our company professional.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, those of ordinary skill in the art should
Understand, can be to technical scheme technical scheme is modified or replaced equivalently, without departing from the essence of technical solution of the present invention
And scope.
Claims (9)
1. a kind of radiation protection containing sweet osmanthus stem cell extract, acne-removing composition, it is characterised in that including following parts by weight
Component:5-10 parts of sweet osmanthus stem cell extract, 6-8 parts of buckbean leaf extract, 3-5 parts of asparagus extract.
2. radiation protection according to claim 1, acne-removing composition, it is characterised in that the sweet osmanthus stem cell extract and
The weight ratio of buckbean leaf extract is 1:1.
3. radiation protection according to claim 1, acne-removing composition, it is characterised in that the sweet osmanthus stem cell extract and
The weight ratio of asparagus extract is 2:1.
4. radiation protection according to claim 1, acne-removing composition, it is characterised in that the anti-acne, net white composition also wrap
Include preservative, pH adjusting agent, thickener, emulsifying agent, essence, pigment and water.
5. radiation protection according to claim 1, acne-removing composition, it is characterised in that the emulsifying agent is selected from cetearyl alcohol
Alcohol polyethers -6, ceteareth -25, glycerin monostearate, it is hard that the emollient is selected from White Mineral Oil, stearic acid, spermaceti
Lipidol, olive oil, octanoic acid/certain herbaceous plants with big flowers acid triglyceride, shea butter, jojoba oil, silicone oil, the skin moisturizer be selected from glycerine,
Propane diols, 1,3 butanediols.
6. a kind of radiation protection as described in claim 1-5 is any, the preparation method of acne-removing composition, it is characterised in that described
Preparation method is by each component raw material precise by weight ratio, is sufficiently mixed and stirs evenly, that is, obtains the composition.
7. radiation protection according to claim 1, acne-removing composition, it is characterised in that the agent of the anti-acne, net white composition
Type is aqua, creme, finish, washing lotion or emulsion.
8. a kind of radiation protection, anti-acne skin care item, it is characterised in that including oil phase, water phase, the water mutually includes such as claim 1-
5 any radiation protections, acne-removing composition.
9. radiation protection according to claim 8, anti-acne skin care item, it is characterised in that the radiation protection, anti-acne skin care item are also
Including essence and preservative.
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Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
Non-Patent Citations (1)
Title |
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王建新 等: "《化妆品植物原料大全》", 30 June 2012 * |
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