CN107927355A - Artificial breeding feed of Sipunculus nudus and preparation method thereof - Google Patents
Artificial breeding feed of Sipunculus nudus and preparation method thereof Download PDFInfo
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- CN107927355A CN107927355A CN201711263091.8A CN201711263091A CN107927355A CN 107927355 A CN107927355 A CN 107927355A CN 201711263091 A CN201711263091 A CN 201711263091A CN 107927355 A CN107927355 A CN 107927355A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
Abstract
The invention discloses a kind of artificial breeding feed of Sipunculus nudus, include the raw material of following parts by weight:21~25 parts of flour, 10~13 parts of dregs of beans, 7~9 portions of corn flour, 5~10 parts of cuttlefish powders, 8~12 parts of dried silkworm chrysalis meals, 4~6 parts of shrimp shell meals, 3~8 parts of liver powder, 25~28 parts of Isochrysis galbanas, 17~22 parts of Chaetoceros, 15~19 parts mini green pepper grass, 2~9 parts of deer horn tongues, 1~6 part of needle spikesedge herb, 11~15 parts of calcium dihydrogen phosphates, 7~13 parts of zeolite powders, 1~4 part of montmorillonite, 1~4 part of bentonite, 3~6 parts of choline chlorides, 5~7 parts of glycine betaines, 16~22 parts of sodium humates, 6~10 parts of allicins, 7~9 parts of composite mineral matters.The present invention can improve Sipunculus nudus artificial breeding survival rate, shorten the culturing time of Sipunculus nudus, improve the yield of Sipunculus nudus.
Description
Technical field
The present invention relates to Sipunculus nudus to cultivate field.It is more particularly related to a kind of Sipunculus nudus is manually supported
Grow feed and preparation method thereof.
Background technology
Sipunculus nudus is commonly called as sandworm, is under the jurisdiction of Genus Golfingia, Genus Sipunculus.China coast has point from south to north
Cloth, wherein more with North Sea distribution, Sipunculus nudus is one of famous marine products treasure, its delicious flavour, nutritive value is high,
According to being documented, containing crude protein 73.77%, ash content 17.44%, total reducing sugar 5.34%, crude fat in Sipunculus nudus individual
0.67%, while the essential amino acid A wide selection of colours and designs contained in Sipunculus nudus, active ingredient therein also have it is antifatigue,
Slow down aging, strengthen effect that is immune, adjusting blood pressure, actually rare excellent tonic product.But Sipunculus nudus is to ring of surviving
Border is especially sensitive, and in recent years since coyoting and the pollution of marine environment cause the yield degradation of Sipunculus nudus, visits
The artificial breeding process of rope Sipunculus nudus and supporting feed, to improve Sipunculus nudus artificial breeding survival rate, shorten grid
The culturing time of siphon-worm, the yield of raising Sipunculus nudus are an extremely urgent things.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
Sipunculus nudus artificial breeding survival rate can be improved it is a still further object of the present invention to provide one kind, shorten grid star
The culturing time of worm, the artificial breeding feed of Sipunculus nudus of yield for improving Sipunculus nudus and preparation method thereof.
In order to realize these purposes and further advantage according to the present invention, there is provided a kind of artificial breeding of Sipunculus nudus is raised
Material, includes the raw material of following parts by weight:21~25 parts of flour, 10~13 parts of dregs of beans, 7~9 portions of corn flour, 5~10 parts of cuttlefish powders,
8~12 parts of dried silkworm chrysalis meals, 4~6 parts of shrimp shell meals, 3~8 parts of liver powder, 25~28 parts of Isochrysis galbanas, 17~22 parts of Chaetoceros, 15
~19 parts mini green pepper grass, 2~9 parts of deer horn tongues, 1~6 part of needle spikesedge herb, 11~15 parts of calcium dihydrogen phosphates, 7~13 parts of zeolite powders, 1~
4 parts of montmorillonites, 1~4 part of bentonite, 3~6 parts of choline chlorides, 5~7 parts of glycine betaines, 16~22 parts of sodium humates, 6~10 portions of garlics
Element, 7~9 parts of composite mineral matters.
Preferably, the raw material of following percentage by weight is included in every part of composite mineral matter:13% cupric glycinate, 25%
Sodium selenite, 17% calcium iodate, 21% manganese methionine, 9% glycine zine, 15% cobalt methionine.
Preferably, mini green pepper grass, deer horn tongue, the weight ratio of needle spikesedge herb are 4.5:1:1.
Preferably, calcium dihydrogen phosphate, zeolite powder, montmorillonite, bentonite, the weight ratio of composite mineral matter are 1:1:0.25:
0.25:0.67.
The present invention also provides a kind of preparation method of the artificial breeding feed of above-mentioned Sipunculus nudus, including:
Step 1: the Isochrysis galbana of above-mentioned parts by weight, Chaetoceros, mini green pepper grass, deer horn tongue and needle spikesedge herb are shredded, obtain
Shred material, material will be shredded and be placed in reaction kettle, then add into reaction kettle the mass fraction for shredding 5 times of gross mass of material for 25~
The aqueous solution of 30% NaCl, carries out first time heating water bath, bath temperature is 90~100 DEG C, when in reaction kettle by reaction kettle
Pressure keeps 30~45min of water-bath after reaching 220~240kpa, then is placed in air dropping naturally by reaction kettle stopping water-bath
Temperature, leaves and takes liquid therein and obtains the first diffusate when reaction kettle is cooled to room temperature, then is added into reaction kettle and shred material gross mass 3
Mass fraction again is the aqueous solution of 15~19% NaCl, and reaction kettle is carried out second of heating water bath, bath temperature 90
~100 DEG C, 24~28min of water-bath is kept after reacting kettle inner pressure reaches 190~210kpa, reaction kettle is then stopped into water-bath
Temperature fall in air is placed in, liquid therein is left and taken when reaction kettle is cooled to room temperature and obtains the second diffusate, while will reaction
In kettle it is remaining shred material air-dry be crushed into powder, obtain powder materials;
Step 2, will be obtained in the choline chloride of above-mentioned parts by weight, glycine betaine, sodium humate, allicin and step 1
First diffusate and the second diffusate mix to obtain soak, then soak is evaporated under reduced pressure, pressure control 65~
75kpa, temperature control is at 80~90 DEG C, when the volume of soak is decreased to original volumeStop vacuum distillation, then will be upper
Powder materials made from stating flour, dregs of beans, corn flour, cuttlefish powder, dried silkworm chrysalis meal, shrimp shell meal, liver powder and the step 1 of parts by weight fall
Enter to stir evenly in the soak after vacuum distillation and paste is made, the calcium dihydrogen phosphate of above-mentioned parts by weight, zeolite powder, illiteracy is de-
Soil, bentonite, composite mineral matter mix to obtain mixture with the deionized water of 112~120 parts by weight, and mixture is kneaded into a diameter of
The graininess of 3~5mm, then put it into Muffle furnace and calcine, calcining heat control at 350~400 DEG C, time control 25~
35min, must calcine material, will calcine the circumferentially uniform applied paste of particle surface of material, enwrapped granule material is obtained, by enwrapped granule material wind
Particulate material must be air-dried after dry;
Step 3: obtained air-dried particulate material in step 2 is carried out disinfection by ultraviolet light, the artificial breeding of Sipunculus nudus is obtained
Feed.
Preferably, ultrasonication is carried out before soak is evaporated under reduced pressure in step 2, the frequency of ultrasonic wave is 28
~32KHz.
The present invention includes at least following beneficial effect:
Containing more protein and trace element, it can meet the nutrient needed for Sipunculus nudus growth comprehensively, be confused at the same time
The active ingredient contained in your green pepper grass, deer horn tongue, needle spikesedge herb can stimulate Sipunculus nudus to grow, and shorten its growth cycle, and corruption is planted
Sipunculus nudus artificial breeding survival rate can be improved after sour sodium, allicin compatibility.And preparation method provided by the invention is according to grid
The life habit of siphon-worm, occupy shoal sand ground for a long time, is fabricated to the little particle with coating, is on the one hand easily settled down to shoal bottom
Portion facilitates Sipunculus nudus to ingest, and the component of another aspect surface coatings also can induce Sipunculus nudus and ingest.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification
Word can be implemented according to this.
It should be noted that experimental method described in following embodiments, is conventional method unless otherwise specified, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
<Embodiment 1>
A kind of artificial breeding feed of Sipunculus nudus, includes the raw material of following parts by weight:21 parts of flour, 10 parts of dregs of beans, 7 parts
Corn flour, 5 parts of cuttlefish powders, 8 parts of dried silkworm chrysalis meals, 4 parts of shrimp shell meals, 3 parts of liver powder, 25 parts of Isochrysis galbanas, 17 parts of Chaetoceros, 15 parts
Mini green pepper grass, 2 parts of deer horn tongues, 1 part of needle spikesedge herb, 11 parts of calcium dihydrogen phosphates, 7 parts of zeolite powders, 1 part of montmorillonite, 1 part of bentonite, 3 parts of chlorine
Change choline, 5 parts of glycine betaines, 16 parts of sodium humates, 6 parts of allicins, 7 parts of composite mineral matters.
Wherein, the raw material of following percentage by weight is included in every part of composite mineral matter:13% cupric glycinate, 25% Asia
Sodium selenate, 17% calcium iodate, 21% manganese methionine, 9% glycine zine, 15% cobalt methionine.
The preparation method of the artificial breeding feed of above-mentioned Sipunculus nudus, including:
Step 1: the Isochrysis galbana of above-mentioned parts by weight, Chaetoceros, mini green pepper grass, deer horn tongue and needle spikesedge herb are shredded, obtain
Material is shredded, material will be shredded and be placed in reaction kettle, then is added into reaction kettle and shreds the mass fraction for expecting 5 times of gross mass as 25%
The aqueous solution of NaCl, carries out first time heating water bath, bath temperature is 90 DEG C, when reacting kettle inner pressure reaches by reaction kettle
Water-bath 30min is kept after 220kpa, reaction kettle stopping water-bath is then placed in Temperature fall in air, when reaction kettle is down to room
Liquid therein is left and taken after temperature and obtains the first diffusate, then the mass fraction that addition shreds 3 times of gross mass of material into reaction kettle is
The aqueous solution of 15% NaCl, carries out second of heating water bath, bath temperature is 90 DEG C, when reacting kettle inner pressure is reached by reaction kettle
Water-bath 24min is kept after to 190kpa, reaction kettle stopping water-bath is then placed in Temperature fall in air, when reaction kettle is down to
Liquid therein is left and taken after room temperature and obtains the second diffusate, at the same by reaction kettle it is remaining shred material and air-dry be crushed into powder, obtain powder
End material;
Step 2, will be obtained in the choline chloride of above-mentioned parts by weight, glycine betaine, sodium humate, allicin and step 1
First diffusate and the second diffusate mix to obtain soak, first carry out ultrasonication to soak, and the frequency of ultrasonic wave is
28KHz, then soak is evaporated under reduced pressure, pressure is controlled in 65kpa, and temperature control is at 80 DEG C, when the volume of soak subtracts
As low as original volumeI.e. stop vacuum distillation, then by the flour of above-mentioned parts by weight, dregs of beans, corn flour, cuttlefish powder, dried silkworm chrysalis meal,
Stirred evenly in the soak that shrimp shell meal, liver powder are poured into after vacuum distillation with powder materials made from step 1 and paste is made, will
Calcium dihydrogen phosphate, zeolite powder, montmorillonite, bentonite, the composite mineral matter of above-mentioned parts by weight are mixed with the deionized water of 112 parts by weight
Mixture is obtained, the graininess by mixture kneading into a diameter of 3mm, then put it into Muffle furnace and calcine, calcining heat control
At 350 DEG C, time control must calcine material, will calcine the circumferentially uniform applied paste of particle surface of material in 25min, must wrap up
Pellet, must air-dry particulate material after enwrapped granule material is air-dried;
Step 3: obtained air-dried particulate material in step 2 is carried out disinfection by ultraviolet light, the artificial breeding of Sipunculus nudus is obtained
Feed.
<Embodiment 2>
A kind of artificial breeding feed of Sipunculus nudus, includes the raw material of following parts by weight:25 parts of flour, 13 parts of dregs of beans, 9 parts
Corn flour, 10 parts of cuttlefish powders, 12 parts of dried silkworm chrysalis meals, 6 parts of shrimp shell meals, 8 parts of liver powder, 28 parts of Isochrysis galbanas, 22 parts of Chaetoceros, 19
The mini green pepper grass of part, 9 parts of deer horn tongues, 6 parts of needle spikesedge herbs, 15 parts of calcium dihydrogen phosphates, 13 parts of zeolite powders, 4 parts of montmorillonites, 4 parts of bentonite, 6
Part choline chloride, 7 parts of glycine betaines, 22 parts of sodium humates, 10 parts of allicins, 9 parts of composite mineral matters.
Wherein, the raw material of following percentage by weight is included in every part of composite mineral matter:13% cupric glycinate, 25% Asia
Sodium selenate, 17% calcium iodate, 21% manganese methionine, 9% glycine zine, 15% cobalt methionine.
The preparation method of the artificial breeding feed of above-mentioned Sipunculus nudus, including:
Step 1: the Isochrysis galbana of above-mentioned parts by weight, Chaetoceros, mini green pepper grass, deer horn tongue and needle spikesedge herb are shredded, obtain
Material is shredded, material will be shredded and be placed in reaction kettle, then is added into reaction kettle and shreds the mass fraction for expecting 5 times of gross mass as 30%
The aqueous solution of NaCl, carries out first time heating water bath, bath temperature is 100 DEG C, when reacting kettle inner pressure reaches by reaction kettle
Water-bath 45min is kept after 240kpa, reaction kettle stopping water-bath is then placed in Temperature fall in air, when reaction kettle is down to room
Liquid therein is left and taken after temperature and obtains the first diffusate, then the mass fraction that addition shreds 3 times of gross mass of material into reaction kettle is
The aqueous solution of 19% NaCl, carries out second of heating water bath, bath temperature is 100 DEG C, works as reacting kettle inner pressure by reaction kettle
Water-bath 28min is kept after reaching 210kpa, reaction kettle stopping water-bath is then placed in Temperature fall in air, when reaction kettle drops
Liquid therein is left and taken after to room temperature and obtains the second diffusate, at the same by reaction kettle it is remaining shred material and air-dry be crushed into powder, obtain
Powder materials;
Step 2, will be obtained in the choline chloride of above-mentioned parts by weight, glycine betaine, sodium humate, allicin and step 1
First diffusate and the second diffusate mix to obtain soak, first carry out ultrasonication to soak, and the frequency of ultrasonic wave is
32KHz, then soak is evaporated under reduced pressure, pressure is controlled in 75kpa, and temperature control is at 90 DEG C, when the volume of soak subtracts
As low as original volumeI.e. stop vacuum distillation, then by the flour of above-mentioned parts by weight, dregs of beans, corn flour, cuttlefish powder, dried silkworm chrysalis meal,
Stirred evenly in the soak that shrimp shell meal, liver powder are poured into after vacuum distillation with powder materials made from step 1 and paste is made, will
Calcium dihydrogen phosphate, zeolite powder, montmorillonite, bentonite, the composite mineral matter of above-mentioned parts by weight are mixed with the deionized water of 120 parts by weight
Mixture is obtained, the graininess by mixture kneading into a diameter of 5mm, then put it into Muffle furnace and calcine, calcining heat control
At 400 DEG C, time control must calcine material, will calcine the circumferentially uniform applied paste of particle surface of material in 35min, must wrap up
Pellet, must air-dry particulate material after enwrapped granule material is air-dried;
Step 3: obtained air-dried particulate material in step 2 is carried out disinfection by ultraviolet light, the artificial breeding of Sipunculus nudus is obtained
Feed.
<Embodiment 3>
A kind of artificial breeding feed of Sipunculus nudus, includes the raw material of following parts by weight:23 parts of flour, 12 parts of dregs of beans, 8 parts
Corn flour, 8 parts of cuttlefish powders, 10 parts of dried silkworm chrysalis meals, 5 parts of shrimp shell meals, 6 parts of liver powder, 27 parts of Isochrysis galbanas, 20 parts of Chaetoceros, 18
The mini green pepper grass of part, 4 parts of deer horn tongues, 4 parts of needle spikesedge herbs, 12 parts of calcium dihydrogen phosphates, 12 parts of zeolite powders, 3 parts of montmorillonites, 3 parts of bentonite, 5
Part choline chloride, 6 parts of glycine betaines, 19 parts of sodium humates, 8 parts of allicins, 8 parts of composite mineral matters.
Wherein, the raw material of following percentage by weight is included in every part of composite mineral matter:13% cupric glycinate, 25% Asia
Sodium selenate, 17% calcium iodate, 21% manganese methionine, 9% glycine zine, 15% cobalt methionine.
The preparation method of the artificial breeding feed of above-mentioned Sipunculus nudus, including:
Step 1: the Isochrysis galbana of above-mentioned parts by weight, Chaetoceros, mini green pepper grass, deer horn tongue and needle spikesedge herb are shredded, obtain
Material is shredded, material will be shredded and be placed in reaction kettle, then is added into reaction kettle and shreds the mass fraction for expecting 5 times of gross mass as 28%
The aqueous solution of NaCl, carries out first time heating water bath, bath temperature is 90~100 DEG C, when reacting kettle inner pressure is reached by reaction kettle
Water-bath 38min is kept after to 230kpa, reaction kettle stopping water-bath is then placed in Temperature fall in air, when reaction kettle is down to
Liquid therein is left and taken after room temperature and obtains the first diffusate, then the mass fraction that addition shreds 3 times of gross mass of material into reaction kettle is
The aqueous solution of 17% NaCl, carries out second of heating water bath, bath temperature is 95 DEG C, when reacting kettle inner pressure is reached by reaction kettle
Water-bath 26min is kept after to 200kpa, reaction kettle stopping water-bath is then placed in Temperature fall in air, when reaction kettle is down to
Liquid therein is left and taken after room temperature and obtains the second diffusate, at the same by reaction kettle it is remaining shred material and air-dry be crushed into powder, obtain powder
End material;
Step 2, will be obtained in the choline chloride of above-mentioned parts by weight, glycine betaine, sodium humate, allicin and step 1
First diffusate and the second diffusate mix to obtain soak, first carry out ultrasonication to soak, and the frequency of ultrasonic wave is
30KHz, then soak is evaporated under reduced pressure, pressure is controlled in 70kpa, and temperature control is at 85 DEG C, when the volume of soak subtracts
As low as original volumeI.e. stop vacuum distillation, then by the flour of above-mentioned parts by weight, dregs of beans, corn flour, cuttlefish powder, dried silkworm chrysalis meal,
Stirred evenly in the soak that shrimp shell meal, liver powder are poured into after vacuum distillation with powder materials made from step 1 and paste is made, will
Calcium dihydrogen phosphate, zeolite powder, montmorillonite, bentonite, the composite mineral matter of above-mentioned parts by weight are mixed with the deionized water of 116 parts by weight
Mixture is obtained, the graininess by mixture kneading into a diameter of 4mm, then put it into Muffle furnace and calcine, calcining heat control
At 370 DEG C, time control must calcine material, will calcine the circumferentially uniform applied paste of particle surface of material in 30min, must wrap up
Pellet, must air-dry particulate material after enwrapped granule material is air-dried;
Step 3: obtained air-dried particulate material in step 2 is carried out disinfection by ultraviolet light, the artificial breeding of Sipunculus nudus is obtained
Feed.
<Comparative example>
A kind of artificial breeding feed of Sipunculus nudus, includes the raw material of following parts by weight:30~35 parts of fish meal, 2~3 points of ferment
Mother, 20~30 parts of dregs of beans, 20~30 parts of peanut meals, 5~15 parts of corns, 2~4 parts of mineral matters.
The artificial breeding feed of above-mentioned Sipunculus nudus is mixed into excessively uniform.
The Sipunculus nudus in above-described embodiment 1, embodiment 2, embodiment 3 and comparative example is propagated artificially feed in the North Sea
Sipunculus nudus cultivation base is tested, and wherein the breeding density of Sipunculus nudus is 20,000/mu, fed 1 time, feeds every 3 days
Amount is continuously fed 3 months in 18g, then digs out Sipunculus nudus, calculate the average weight of Sipunculus nudus per acre, survival rate and into
Worm ratio, the results are shown in Table 1.
Table 1, the average weight of Sipunculus nudus, survival rate and adult ratio contrast table per acre
Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example | |
Weight (kg/ mus) | 216 | 220 | 226 | 183 |
Survival rate (%/mu) | 93 | 96 | 97 | 73 |
Adult ratio (%/mu) | 86 | 88 | 90 | 62 |
It is not difficult to find out in embodiment 1~3 that averagely the weight of Sipunculus nudus, survival rate and adult ratio are high per acre from table 1
In comparative example, illustrate that the artificial breeding feed of Sipunculus nudus provided by the invention can improve Sipunculus nudus artificial breeding and survive
Rate, the culturing time for shortening Sipunculus nudus, the yield for improving Sipunculus nudus.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Realize other modification, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details and shown here as the embodiment with description.
Claims (6)
1. the artificial breeding feed of a kind of Sipunculus nudus, it is characterised in that include the raw material of following parts by weight:21~25 parts of faces
Powder, 10~13 parts of dregs of beans, 7~9 portions of corn flour, 5~10 parts of cuttlefish powders, 8~12 parts of dried silkworm chrysalis meals, 4~6 parts of shrimp shell meals, 3~8 parts
Liver powder, 25~28 parts of Isochrysis galbanas, 17~22 parts of Chaetoceros, 15~19 parts mini green pepper grass, 2~9 parts of deer horn tongues, 1~6 part
Needle spikesedge herb, 11~15 parts of calcium dihydrogen phosphates, 7~13 parts of zeolite powders, 1~4 part of montmorillonite, 1~4 part of bentonite, 3~6 parts of chlorination courages
Alkali, 5~7 parts of glycine betaines, 16~22 parts of sodium humates, 6~10 parts of allicins, 7~9 parts of composite mineral matters.
2. the artificial breeding feed of Sipunculus nudus as claimed in claim 1, it is characterised in that included in every part of composite mineral matter
The raw material of following percentage by weight:13% cupric glycinate, 25% sodium selenite, 17% calcium iodate, 21% methionine
Manganese, 9% glycine zine, 15% cobalt methionine.
3. the artificial breeding feed of Sipunculus nudus as claimed in claim 1, it is characterised in that mini green pepper grass, deer horn tongue, ox hair
The weight ratio of felt is 4.5:1:1.
4. the artificial breeding feed of Sipunculus nudus as claimed in claim 1, it is characterised in that calcium dihydrogen phosphate, zeolite powder, illiteracy
De- soil, bentonite, the weight ratio of composite mineral matter are 1:1:0.25:0.25:0.67.
A kind of 5. preparation method of the artificial breeding feed of Sipunculus nudus as claimed in claim 1, it is characterised in that including:
Step 1: the Isochrysis galbana of above-mentioned parts by weight, Chaetoceros, mini green pepper grass, deer horn tongue and needle spikesedge herb are shredded, must shred
Material, will shred material and is placed in reaction kettle, then is added into reaction kettle and shred the mass fraction for expecting 5 times of gross mass as 25~30%
The aqueous solution of NaCl, carries out first time heating water bath, bath temperature is 90~100 DEG C, when reacting kettle inner pressure is reached by reaction kettle
30~45min of water-bath is kept after to 220~240kpa, reaction kettle stopping water-bath is then placed in Temperature fall in air, when
Reaction kettle, which is cooled to room temperature, to be left and taken liquid therein and obtains the first diffusate, then is added into reaction kettle and shredded 3 times of gross mass of material
Mass fraction is the aqueous solution of 15~19% NaCl, reaction kettle is carried out second of heating water bath, bath temperature is 90~100
DEG C, 24~28min of water-bath is kept after reacting kettle inner pressure reaches 190~210kpa, reaction kettle is then stopped into water-bath juxtaposition
The Temperature fall in air, leaves and takes liquid therein and obtains the second diffusate when reaction kettle is cooled to room temperature, while by reaction kettle
It is remaining shred material air-dry be crushed into powder, obtain powder materials;
Step 2, by the choline chloride of above-mentioned parts by weight, glycine betaine, sodium humate, allicin and step 1 obtained first
Diffusate and the second diffusate mix to obtain soak, then soak is evaporated under reduced pressure, and pressure is controlled in 65~75kpa,
Temperature control is at 80~90 DEG C, when the volume of soak is decreased to original volumeStop vacuum distillation, then by above-mentioned weight
Part flour, dregs of beans, corn flour, cuttlefish powder, dried silkworm chrysalis meal, shrimp shell meal, powder materials pour into decompression made from liver powder and step 1
Stirred evenly in soak after distillation and paste be made, by the calcium dihydrogen phosphate of above-mentioned parts by weight, zeolite powder, montmorillonite, bentonite,
Composite mineral matter mixes to obtain mixture with the deionized water of 112~120 parts by weight, and mixture is kneaded into a diameter of 3~5mm's
Graininess, then put it into Muffle furnace and calcine, at 350~400 DEG C, time control is obtained in 25~35min for calcining heat control
Calcining material, will calcine the circumferentially uniform applied paste of particle surface of material, and obtain enwrapped granule material, wind is obtained after enwrapped granule material is air-dried
Dry particl material;
Step 3: obtained air-dried particulate material in step 2 is carried out disinfection by ultraviolet light, the artificial breeding feed of Sipunculus nudus is obtained.
6. the preparation method of the artificial breeding feed of Sipunculus nudus as claimed in claim 5, it is characterised in that soaked in step 2
Bubble liquid carries out ultrasonication before being evaporated under reduced pressure, and the frequency of ultrasonic wave is 28~32KHz.
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CN109055384A (en) * | 2018-07-19 | 2018-12-21 | 华侨大学 | A kind of Sipunculus nudus beta-actin gene and its application |
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