CN107915516A - Use the selenium-rich sweet potato implantation methods of tissue-cultured seedling - Google Patents
Use the selenium-rich sweet potato implantation methods of tissue-cultured seedling Download PDFInfo
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- CN107915516A CN107915516A CN201711119704.0A CN201711119704A CN107915516A CN 107915516 A CN107915516 A CN 107915516A CN 201711119704 A CN201711119704 A CN 201711119704A CN 107915516 A CN107915516 A CN 107915516A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention discloses a kind of selenium-rich sweet potato implantation methods using tissue-cultured seedling, comprise the following steps：Step 1: prepare soil matrix；Step 2: it is explant to choose sweet potato main bud, after soaking 20 30 minutes with sterile water, then it is placed in the liquor natrii hypochloritis that mass fraction is 20 25% and sterilizes 5 minutes, take out the culture for after 46 minutes, being put into aseptic water washing and tissue-cultured seedling being carried out in culture medium；Step 3: when tissue-cultured seedling length is to 5 6cm, will be cultivated in tissue culture transplantation of seedlings to soil matrix；Step 4: when tissue-cultured seedling length is to 10 12cm, by tissue culture transplantation of seedlings to plantation field；Step 5: after tissue-cultured seedling length is to more than 15cm, a Selenium-rich nutrient solution is sprayed into plantation field within every 10 12 days.The object of the present invention is to provide a kind of selenium-rich sweet potato implantation methods using tissue-cultured seedling.
Description
Technical field
The present invention relates to field of plant tissue culture.It is more particularly related to a kind of selenium-rich using tissue-cultured seedling
Sweet potato implantation methods.
Background technology
Purple sweep potato introduces China by Japan, and black potato, purple sweet potato, purple sweet potato, purple sweet potato etc. are also known as in China.Mesh
Before, the high-quality purple sweep potato kind of China market Introduced From Abroad includes：American Black sweet potato, German black potato, Japanese river mountain purple etc.；From
The purple sweep potato kind for the suitable domestic plantation that row is cultivated includes：Rhizoma Dioscoreae esculentae No. 18, wide potato 135, peaceful purple No. 4, capital potato 6
Number, Chongqing is No. 1 purple, Xu's potato 4 and southern purple 008 etc..The planting area of China's purple sweep potato be concentrated mainly on northeast,
Zhejiang, Anhui, Henan, Shandong, Guangdong, Yunnan, Hainan etc. save, and the tessellated region centered on Jiangsu-zhejiang Shanghai Area is the master of purple sweep potato
Want consumption market.In recent years, continually developing with purple sweep potato and Related product, makes purple sweep potato obtain more and more
Concern, its planting scale and economic value are all constantly being lifted.
Selenium is the essential trace elements of the human body.Chinese Soclety of Nutrition also by selenium be classified as 15 kinds of nutrients needed by human it
One,
A large amount of clinical trials both at home and abroad are bright, and human body selenium deficiency can cause the functional disturbance of some vitals, cause many tight
Disease is weighed, more than 40, whole world country is in selenium deficiency area, and several hundred million populations in Chinese 22 provinces are all in selenium deficiency or low
The incidence such as selenium area, these regional population tumour, hepatopathy, angiocardiopathies is very high.
The content of the invention
The object of the present invention is to provide a kind of selenium-rich sweet potato implantation methods using tissue-cultured seedling.
In order to realize these purposes and further advantage according to the present invention, there is provided a kind of selenium-rich sweet potato using tissue-cultured seedling
Implantation methods, comprise the following steps：
Step 1: after rural area soil, sandy soil and selenium mineral powder are respectively placed in being exposed to the sun 5-7 days under sunlight, by rural area soil, sandy soil and
Selenium mineral powder is 70 according to mass ratio:20:1 ratio is uniformly mixed, and is added thimerosal and is evenly stirred until moistening, obtains soil base
Matter；
Step 2: it is explant to choose sweet potato main bud, after being soaked 20-30 minutes with sterile water, then it is placed in mass fraction and is
The liquor natrii hypochloritis of 20-25% sterilizes 5 minutes, takes out and after 4-6 minutes, is put into culture medium with aseptic water washing and carries out tissue culture
The culture of seedling；
Step 3: when tissue-cultured seedling length is to 5-6cm, will be cultivated in tissue culture transplantation of seedlings to soil matrix；
Step 4: when tissue-cultured seedling length is to 10-12cm, by tissue culture transplantation of seedlings to plantation field；
Step 5: after tissue-cultured seedling length is to more than 15cm, a Selenium-rich nutrient solution was sprayed into plantation field per 10-12 days,
Wherein, the Selenium-rich nutrient solution is made of the component of following parts by weight：
10-20 parts of potassium phosphate, 10-15 parts of potassium sulfate, 2-3 parts of boric acid, 2-5 parts of manganese sulfate, 1-2 parts of zinc sulfate, selenous acid
1000 parts of 5-8 parts of sodium, 2-5 parts of calcium superphosphate and water.
Preferably, in the selenium-rich sweet potato implantation methods using tissue-cultured seedling, the culture that is used in the step 2
Base includes：The 6-benzyladenine of MS, 2-3mg/L, the methyl α-naphthyl acetate of 0.5-0.8mg/L, 20-25g/L sucrose and 10-15g/L
Agar.
Preferably, in the selenium-rich sweet potato implantation methods using tissue-cultured seedling, the initial pH value of the culture medium is
5-6。
Preferably, in the selenium-rich sweet potato implantation methods using tissue-cultured seedling, culture medium is used in the step 2
During the culture of middle progress tissue-cultured seedling, cultivation temperature is 23-27 DEG C, intensity of illumination 1200-2000lux, light application time 8-12
Hour/day.
Preferably, in the selenium-rich sweet potato implantation methods using tissue-cultured seedling, by tissue culture transplantation of seedlings to plantation field
The previous moon, plantation field is deep ploughed 40-50 centimetres, then applies soil sterilants, after soil disinfection 7 days, is applied into plantation field
It is 1 in mass ratio to add by selenium mineral powder and metallurgical ground-slag:80-120kg/ mus of the mineral powder that 1-5 is mixed.
The selenium-rich sweet potato implantation methods using tissue-cultured seedling of the present invention improve the survival rate of sweet potato tissue-cultured seedling, saved into
This, improves economic benefit.
The Selenium-rich nutrient solution that the present invention uses can not only meet nutrition needed for growth of sweet potato, additionally it is possible to selenium element is supplemented,
Improve the Se content of sweet potato stem tuber.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
<Embodiment 1>
A kind of selenium-rich sweet potato implantation methods using tissue-cultured seedling, comprise the following steps：
Step 1: after rural area soil, sandy soil and selenium mineral powder are respectively placed in being exposed to the sun 5 days under sunlight, by rural area soil, sandy soil and selenium
Miberal powder is 70 according to mass ratio:20:1 ratio is uniformly mixed, and is added thimerosal and is evenly stirred until moistening, obtains soil base
Matter；
Step 2: it is explant to choose sweet potato main bud, with sterile water immersion after twenty minutes, then mass fraction is placed in as 20%
Liquor natrii hypochloritis sterilize 5 minutes, take out with aseptic water washing after 4 minutes, be put into the culture that tissue-cultured seedling is carried out in culture medium,
The culture medium includes：The 6-benzyladenine of MS, 2mg/L, the methyl α-naphthyl acetate of 0.5mg/L, the agar of 20g/L sucrose and 10g/L,
The initial pH value of culture medium is 5, and cultivation temperature is 23 DEG C, intensity of illumination 1200lux, when light application time is 8 small/day.；
Step 3: when tissue-cultured seedling length is to 5cm, will be cultivated in tissue culture transplantation of seedlings to soil matrix；
Step 4: when tissue-cultured seedling length is to 10cm, by tissue culture transplantation of seedlings to plantation field；
Step 5: after tissue-cultured seedling length is to more than 15cm, a Selenium-rich nutrient solution is sprayed into plantation field within every 10 days, its
In, the Selenium-rich nutrient solution is made of the component of following parts by weight：
10 parts of potassium phosphate, 10 parts of potassium sulfate, 2 parts of boric acid, 2 parts of manganese sulfate, 1 part of zinc sulfate, 5 parts of sodium selenite, peroxophosphoric acid
1000 parts of 2 parts of calcium and water.
Wherein, tissue culture transplantation of seedlings is deep ploughed in plantation field 40 centimetres, then apply soil disinfection to the previous moon in field is planted
Agent, after soil disinfection 7 days, it is 1 in mass ratio to apply into plantation field by selenium mineral powder and metallurgical ground-slag:1 ore deposit mixed
80kg/ mus of thing powder.
<Embodiment 2>
A kind of selenium-rich sweet potato implantation methods using tissue-cultured seedling, comprise the following steps：
Step 1: after rural area soil, sandy soil and selenium mineral powder are respectively placed in being exposed to the sun 6 days under sunlight, by rural area soil, sandy soil and selenium
Miberal powder is 70 according to mass ratio:20:1 ratio is uniformly mixed, and is added thimerosal and is evenly stirred until moistening, obtains soil base
Matter；
Step 2: it is explant to choose sweet potato main bud, after being soaked 25 minutes with sterile water, then mass fraction is placed in as 22%
Liquor natrii hypochloritis sterilize 5 minutes, take out with aseptic water washing after 4-6 minute, be put into the training of progress tissue-cultured seedling in culture medium
Support, the culture medium includes：The 6-benzyladenine of MS, 2.5mg/L, the methyl α-naphthyl acetate of 0.65mg/L, 22g/L sucrose and 12g/L
Agar, the initial pH value of culture medium is 5, and cultivation temperature is 25 DEG C, intensity of illumination 1600lux, light application time for 10 it is small when/
My god.；
Step 3: when tissue-cultured seedling length is to 5cm, will be cultivated in tissue culture transplantation of seedlings to soil matrix；
Step 4: when tissue-cultured seedling length is to 11cm, by tissue culture transplantation of seedlings to plantation field；
Step 5: after tissue-cultured seedling length is to more than 15cm, a Selenium-rich nutrient solution is sprayed into plantation field within every 11 days, its
In, the Selenium-rich nutrient solution is made of the component of following parts by weight：
15 parts of potassium phosphate, 12.5 parts of potassium sulfate, 2.5 parts of boric acid, 3.5 parts of manganese sulfate, 1.5 parts of zinc sulfate, sodium selenite 6.5
1000 parts of part, 3.5 parts of calcium superphosphate and water.
Wherein, tissue culture transplantation of seedlings is deep ploughed in plantation field 45 centimetres, then apply soil disinfection to the previous moon in field is planted
Agent, after soil disinfection 7 days, it is 1 in mass ratio to apply into plantation field by selenium mineral powder and metallurgical ground-slag:3 ore deposits mixed
100kg/ mus of thing powder.
<Embodiment 3>
A kind of selenium-rich sweet potato implantation methods using tissue-cultured seedling, comprise the following steps：
Step 1: after rural area soil, sandy soil and selenium mineral powder are respectively placed in being exposed to the sun 7 days under sunlight, by rural area soil, sandy soil and selenium
Miberal powder is 70 according to mass ratio:20:1 ratio is uniformly mixed, and is added thimerosal and is evenly stirred until moistening, obtains soil base
Matter；
Step 2: it is explant to choose sweet potato main bud, after being soaked 30 minutes with sterile water, then mass fraction is placed in as 25%
Liquor natrii hypochloritis sterilize 5 minutes, take out with aseptic water washing after 6 minutes, be put into the culture that tissue-cultured seedling is carried out in culture medium,
The culture medium includes：The 6-benzyladenine of MS, 3mg/L, the methyl α-naphthyl acetate of 0.8mg/L, the agar of 25g/L sucrose and 15g/L,
The initial pH value of culture medium is 6, and cultivation temperature is 27 DEG C, intensity of illumination 2000lux, when light application time is 12 small/day.；
Step 3: when tissue-cultured seedling length is to 6cm, will be cultivated in tissue culture transplantation of seedlings to soil matrix；
Step 4: when tissue-cultured seedling length is to 12cm, by tissue culture transplantation of seedlings to plantation field；
Step 5: after tissue-cultured seedling length is to more than 15cm, a Selenium-rich nutrient solution is sprayed into plantation field within every 12 days, its
In, the Selenium-rich nutrient solution is made of the component of following parts by weight：
20 parts of potassium phosphate, 15 parts of potassium sulfate, 3 parts of boric acid, 5 parts of manganese sulfate, 2 parts of zinc sulfate, 8 parts of sodium selenite, peroxophosphoric acid
1000 parts of 5 parts of calcium and water.
Wherein, tissue culture transplantation of seedlings is deep ploughed in plantation field 50 centimetres, then apply soil disinfection to the previous moon in field is planted
Agent, after soil disinfection 7 days, it is 1 in mass ratio to apply into plantation field by selenium mineral powder and metallurgical ground-slag:5 ore deposits mixed
120kg/ mus of thing powder.
<Comparative example 1>
In step 5, ordinary nutritional liquid is sprayed into plantation field, remaining parameter is with identical in example 1, process
It is identical.
<Comparative example 2>
In step 1, soil matrix is 7 according to mass ratio by rural area soil and sandy soil:2 ratio is uniformly mixed, remaining parameter
With identical in example 2, process is also identical.
Table 1 plants the germination percentage of the sweet potato stem tuber Se content that field harvests every year and tissue-cultured seedling per acre
As shown in Table 1, using the present invention selenium-rich sweet potato implantation methods using tissue-cultured seedling obtained sweet potato stem tuber
Se content higher.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Realize other modification, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details and shown here as the embodiment with description.
Claims (5)
1. a kind of selenium-rich sweet potato implantation methods using tissue-cultured seedling, it is characterised in that comprise the following steps：
Step 1: after rural area soil, sandy soil and selenium mineral powder are respectively placed in being exposed to the sun 5-7 days under sunlight, by rural area soil, sandy soil and selenium ore deposit
Powder is 70 according to mass ratio:20:1 ratio is uniformly mixed, and is added thimerosal and is evenly stirred until moistening, obtains soil matrix；
Step 2: it is explant to choose sweet potato main bud, after being soaked 20-30 minutes with sterile water, then it is 20- to be placed in mass fraction
25% liquor natrii hypochloritis sterilizes 5 minutes, takes out and after 4-6 minutes, is put into culture medium with aseptic water washing and carries out tissue-cultured seedling
Culture；
Step 3: when tissue-cultured seedling length is to 5-6cm, will be cultivated in tissue culture transplantation of seedlings to soil matrix；
Step 4: when tissue-cultured seedling length is to 10-12cm, by tissue culture transplantation of seedlings to plantation field；
Step 5: after tissue-cultured seedling length is to more than 15cm, a Selenium-rich nutrient solution was sprayed into plantation field per 10-12 days, wherein,
The Selenium-rich nutrient solution is made of the component of following parts by weight：
10-20 parts of potassium phosphate, 10-15 parts of potassium sulfate, 2-3 parts of boric acid, 2-5 parts of manganese sulfate, 1-2 parts of zinc sulfate, sodium selenite 5-8
1000 parts of part, 2-5 parts of calcium superphosphate and water.
2. the selenium-rich sweet potato implantation methods of tissue-cultured seedling are used as claimed in claim 1, it is characterised in that make in the step 2
Culture medium includes：The 6-benzyladenine of MS, 2-3mg/L, the methyl α-naphthyl acetate of 0.5-0.8mg/L, 20-25g/L sucrose and 10-
The agar of 15g/L.
3. the selenium-rich sweet potato implantation methods of tissue-cultured seedling are used as claimed in claim 2, it is characterised in that at the beginning of the culture medium
Beginning pH value is 5-6.
4. the selenium-rich sweet potato implantation methods of tissue-cultured seedling are used as claimed in claim 1, it is characterised in that make in the step 2
During with the culture that tissue-cultured seedling is carried out in culture medium, cultivation temperature is 23-27 DEG C, intensity of illumination 1200-2000lux, during illumination
Between for 8-12 it is small when/day.
5. the selenium-rich sweet potato implantation methods of tissue-cultured seedling are used as claimed in claim 1, it is characterised in that by tissue culture transplantation of seedlings extremely
The previous moon in field is planted, plantation field is deep ploughed 40-50 centimetres, then applies soil sterilants, after soil disinfection 7 days, to kind
It is 1 in mass ratio to apply in plant field by selenium mineral powder and metallurgical ground-slag:80-120kg/ mus of the mineral powder that 1-5 is mixed.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109496707A (en) * | 2018-12-20 | 2019-03-22 | 广西壮族自治区农业科学院生物技术研究所 | A kind of cultural method of selenium-enriched officinal dendrobium stem |
| CN109997640A (en) * | 2019-04-04 | 2019-07-12 | 广西壮族自治区农业科学院 | Selenium-rich sweet potato cultivation technology |
| CN112753513A (en) * | 2021-02-05 | 2021-05-07 | 湖北恩施中国南方马铃薯研究中心 | Water culture rapid propagation method of selenium-rich leaf vegetable type sweet potatoes |
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| CN105850461A (en) * | 2016-04-25 | 2016-08-17 | 吉林师范大学 | Transplanting method for purple sweet potato seedlings |
| CN106576736A (en) * | 2016-11-16 | 2017-04-26 | 唐滨 | High-yield planting method of edible canna rich in selenium |
| CN106717777A (en) * | 2016-11-16 | 2017-05-31 | 唐滨 | Use the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling |
| CN106912275A (en) * | 2017-01-17 | 2017-07-04 | 陆川县米场镇合美种养专业合作社联合社 | A kind of high-yield planting method of selenium-enriched sweet potato |
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| CN103202232A (en) * | 2012-09-26 | 2013-07-17 | 海南省农业科学院粮食作物研究所 | Method for efficiently and industrially producing sweet potato detoxification tissue culture seedlings |
| CN105850461A (en) * | 2016-04-25 | 2016-08-17 | 吉林师范大学 | Transplanting method for purple sweet potato seedlings |
| CN106576736A (en) * | 2016-11-16 | 2017-04-26 | 唐滨 | High-yield planting method of edible canna rich in selenium |
| CN106717777A (en) * | 2016-11-16 | 2017-05-31 | 唐滨 | Use the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109496707A (en) * | 2018-12-20 | 2019-03-22 | 广西壮族自治区农业科学院生物技术研究所 | A kind of cultural method of selenium-enriched officinal dendrobium stem |
| CN109496707B (en) * | 2018-12-20 | 2021-06-04 | 广西壮族自治区农业科学院生物技术研究所 | Cultivation method of selenium-rich dendrobium officinale |
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| CN112753513A (en) * | 2021-02-05 | 2021-05-07 | 湖北恩施中国南方马铃薯研究中心 | Water culture rapid propagation method of selenium-rich leaf vegetable type sweet potatoes |
| CN112753513B (en) * | 2021-02-05 | 2022-12-23 | 湖北恩施中国南方马铃薯研究中心 | Water culture rapid propagation method of selenium-rich leaf vegetable type sweet potatoes |
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