CN106717777A - Use the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling - Google Patents

Use the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling Download PDF

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CN106717777A
CN106717777A CN201611030422.9A CN201611030422A CN106717777A CN 106717777 A CN106717777 A CN 106717777A CN 201611030422 A CN201611030422 A CN 201611030422A CN 106717777 A CN106717777 A CN 106717777A
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selenium
rich
lotus root
seedling
days
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唐滨
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唐滨
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions

Abstract

The invention discloses a kind of implantation methods of the selenium-rich drought lotus root of use tissue-cultured seedling, comprise the following steps:Set up irrigation and drainage water system;Apply mineral powder and selenium-rich base fertilizer;Fine sand and wood chip are spread fertilizer over the fields before transplanting;Dig plant hole, field planting;When lotus root seedling it is long to 10 15 centimetres when spray Selenium-rich nutrient solution, after lotus root seedling is emerged 30 35 days, apply selenium-rich fertilizer.The present invention carries out the cultivation management of science, apply selenium mineral powder and selenium-rich base fertilizer to plantation field in site preparation, and applying Selenium-rich nutrient solution and selenium-rich fertilizer in planting process, not only yield is high but also rich in selenium element for the non-irrigated lotus root stem tuber of results, with high nutritive value and economic worth.

Description

Use the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling
Technical field
The present invention relates to proportion of crop planting field.It is more particularly related to a kind of selenium-rich drought of use tissue-cultured seedling The implantation methods of lotus root.
Background technology
Non-irrigated lotus root, also known as banana dasheen, queensland arrowrood etc., it is the plant of Lowiaceae canna category, the ground such as South America are originated in, on Middle of century introduces China's cultivation.Non-irrigated lotus root has turned into the new raw material sources of high value starch, dry lotus root starch particle in Asia Footpath is big, and gelatinization point is low, and paste transparency is good, and chain content of starch is high, good film-forming property, and its molecular weight is also very big, with farina It is close, with preferable application feature.The exploitation of non-irrigated lotus root to produce based on starch, and develop starch deep processed product and The food items such as bean vermicelli, vermicelli.Dry lotus root starch is also widely adopted in more areas, and general 2.5 kilograms of dry lotus root starch can give birth to 0.5 kilogram of monosodium glutamate is produced, the kind of starch such as oral and glucose for injection, Sorghum vulgare Pers. Maltose sugar can be also produced, and it is various for lotus root starch etc. Non-staple food.In textile industry, dry lotus root starch sizing is used, than using foodstuff starch sizing, with caking ability is high, the spy such as finish is good Point.Dry lotus root starch can be additionally used in papermaking, shoemaking, hatting, clothes and cultural goods etc..
Selenium is the essential trace elements of the human body.Chinese Soclety of Nutrition also by selenium be classified as 15 kinds of nutrients needed by human it One, a large amount of clinical trial both at home and abroad are bright, and human body selenium deficiency can cause the functional disturbance of some vitals, cause many serious diseases Occur, the country of the whole world more than 40 in selenium deficiency area, Chinese 22 several hundred million populations in province all in selenium deficiency or low selenium area, The incidences of disease such as these regional population tumour, hepatopathy, angiocardiopathies are very high.
The non-irrigated lotus root of domestic plantation is mainly distributed on the ground such as Guangxi, Yunnan and Guizhou at present, with field, Around the house nature Based on growth, managed due to lacking, the yield of non-irrigated lotus root is relatively low, and quality is also uneven.And the soil on the ground such as Guangxi, Yunnan and Guizhou Se content is not high in earth, so the Se content of the crops in above-mentioned area is not also high.
The content of the invention
It is an object of the invention to provide a kind of implantation methods of the selenium-rich drought lotus root of use tissue-cultured seedling, obtained using tissue cultures Non-irrigated lotus root seedling, and the cultivation management of science is carried out to non-irrigated lotus root, apply selenium mineral powder and selenium-rich base fertilizer to plantation field in site preparation, and planting Apply Selenium-rich nutrient solution and selenium-rich fertilizer during plant, not only yield is high but also rich in selenium element for the non-irrigated lotus root stem tuber of results, with pole Nutritive value high and economic worth.
In order to realize these purposes of the invention and further advantage, there is provided a kind of selenium-rich drought lotus root of use tissue-cultured seedling Implantation methods, comprise the following steps:
Irrigation and drainage water system is set up in a, the selection field that soil layer is deep, loosing soil is fertile as plantation field;
B, non-irrigated lotus root transplant first trimester, after 40-50 centimetres of plantation field deep ploughing, applying soil sterilants, to soil disinfection In mass ratio it is 1 to applying in plantation field by selenium mineral powder and metallurgical ground-slag after 5-7 days:The mineral powder 80- that 1-5 is mixed 120kg/ mus, to soil disinfection 10-15 days after, to plantation field in apply calcium and magnesium potassium soil conditioner or calcium potassium soil conditioner 20-40kg/ mus, deep plough 40-50 centimetres again, transplant the previous moon, to applying selenium-rich base fertilizer 1200-1500kg/ in plantation field After mu, deep plough 40-50 centimetres;
Wherein, the preparation method of the selenium-rich base fertilizer, comprises the following steps:
Step one, the root of water hyacinth is placed in mass fraction be 1-3% sodium selenite aqueous solution in, daily sooner or later to It is the sodium selenite aqueous solution of 45-50% that mass fraction is sprayed in leaves of hyacinth, after 20-30 days, gathers in water hyacinth and shreds, and is sent out After ferment 2-3 days, selenium-rich green manure is obtained, it is standby;
Step 2, well-rotted farmyard manure is placed under sunlight and be exposed to the sun after 2-3 days, crushed, dry farm manure is obtained, it is standby;
Step 3, by the stalk of 100-200 weight portions, the peanut meal of 100-200 weight portions, 100-200 weight portions sesame The numb dregs of rice, the saccharomycete of the black soya bean dregs of rice, the Trichoderma of 10-20 weight portions and 10-20 weight portions of 100-200 weight portions are well mixed Afterwards, the clear water of 300-600 weight portions is added, is stirred, after fermenting 7-15 days, the bamboo charcoal sponge of addition 30-50 weight portions, In the step of 300-500 weight portions one two the step of obtained selenium-rich green manure, 1200-1500 weight portions in obtained dry farm manure With the Mixed Microbes of 20-50 weight portions, stir, ferment 15-20 days, obtain final product the selenium-rich base fertilizer;
Wherein, the Mixed Microbes are by silicate bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen-fixing bacteria, Verticillium lecanii, whiterot fungi and ferment Female bacterium is 5 in mass ratio:3:3:2:1:1:2 mix;
When c, non-irrigated lotus root tissue-cultured seedling grow 3-5 piece leaves, transplanted;
7-10 days before d, transplanting, after 20-30 centimetres of field deep ploughing will be planted, it is exposed to the sun 2-3 days, fine sand 100- is spread fertilizer over the fields afterwards After 120kg/ mus and wood chip 80-100kg/ mus, the topsoil with plantation field is well mixed;
E, in plantation field dig plant hole, plant hole is deep 2-4 centimetres, and line-spacing is 70-100 centimetres, and spacing in the rows is 50-60 centimetres;
F, when lotus root seedling it is long to 10-15 centimetres when, sprayed Selenium-rich nutrient solution to planting in field per 7-10 days, lotus root seedling is emerged 30- After 35 days, 50-80kg/ mus of selenium-rich fertilizer is applied to planting in field;
Wherein, the Selenium-rich nutrient solution is made up of the component of following parts by weight:
Potassium dihydrogen phosphate 3-5 parts, potassium sulfate 5-10 parts, boric acid 1-2 parts, manganese chloride 1-2 parts, zinc sulfate 0.5-1 parts, sub- selenium Sour sodium or sodium selenate 3-5 parts, calcium superphosphate 2-5 parts and water 800-1000 parts;
The selenium-rich fertilizer is made up of the component of following parts by weight:
Nitrogen-phosphorus-potassium compound fertilizer 12-15 parts, sodium selenite 2-3 parts, glutamic acid 0.2-0.3 parts, tryptophan 0.2-0.3 parts and boron It is fertile 2-3 parts.
Preferably, in the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the metallurgical slag is blast furnace slag, turns Any one or a few in slag and electroslag.
Preferably, in the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the tissue of the non-irrigated lotus root tissue-cultured seedling Cultural method is comprised the following steps:
Step one), by the stem tuber mass fraction of kind of lotus root be 70% alcohol disinfecting after, be put into sterilized water and soak 1-3 Minute, then by the alcohol disinfecting that the stem apex part mass fraction of the stem tuber of kind of lotus root is 75%, then with aseptic water washing, repeat After 3-5 times, sterilized 5-10 minutes with liquor natrii hypochloritis that mass fraction is 5-20%, with aseptic water washing 3-5 times after, be put into Soaked 20-30 minutes in sterilized water, obtain explant;
Step 2), by step one) in the explant that obtains be put into the first culture medium in cultivate, prior to 25-30 DEG C, and every It it is 23-28 DEG C in temperature after being cultivated 5-8 days under conditions of exhausted light, intensity of illumination is 1200-1800lux, and light application time is 8- Cultivated 15-20 days under conditions of 10 hours/day, obtain the first aseptic seedling;
Wherein, first culture medium includes:The 6-benzyladenine of MS, 2-3mg/L, the 2,4- bis- of 0.3-0.8mg/L The agar of chlorophenoxyacetic acid, the gibberellin of 0.3-0.8mg/L, 25-35g/L sucrose and 6-10g/L;
Step 3), by step 2) in the first aseptic seedling for obtaining be placed in the second culture medium, be 23-28 in cultivation temperature DEG C, intensity of illumination is 1200-2000lux, and light application time obtains the second nothing to be cultivated 15-20 days under conditions of 8-12 hours/day Vaccine;
Wherein, second culture medium includes:The 6-benzyladenine of MS, 2-3mg/L, the methyl α-naphthyl acetate of 0.4-0.6mg/L, The agar of the TDZ, 25-35g/L sucrose and 6-10g/L of 0.5-2.0mg/L;
Step 4), by step 3) in the second aseptic seedling for obtaining be placed in the 3rd culture medium, be 23-27 in cultivation temperature DEG C, intensity of illumination is 1200-2000lux, and light application time obtains the 3rd nothing to be cultivated 15-20 days under conditions of 8-12 hours/day Vaccine;
Wherein, the 3rd culture medium includes:The 6-benzyladenine of 1/2MS, 1.5-3mg/L, the naphthalene of 0.4-0.6mg/L The agar of acetic acid, the gibberellin of 0.3-0.8mg/L, 25-35g/L sucrose and 6-10g/L.
Preferably, in the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the tissue of the non-irrigated lotus root tissue-cultured seedling Cultural method is further comprising the steps of:
Step 5), whne step 4) in the 3rd aseptic seedling that the obtains piece leaf to 3-4 long when, hardening 4-7 days is aseptic to the 3rd When seedling hardening grows 3-5 piece leaves, you can transplanted.
Preferably, in the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, first culture medium, described the The initial pH value of two culture mediums and the 3rd culture medium is 5-6.
Preferably, in the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, also include:
G, the first tenday period of a month in April, in lotus root seedling gap green manuring crop in the ranks, after green manure crop harvests, green manure crop is buried In in plantation field.
The present invention at least includes following beneficial effect:
The present invention, to selenium mineral powder is applied in plantation field, increases the Se content of soil in site preparation, and applies selenium-rich base fertilizer, Increase the Se content of soil while improving plantation field soil fertility, before non-irrigated lotus root is planted, improve the selenium of soil in plantation field Content.
The Selenium-rich nutrient solution and selenium-rich fertilizer that the present invention is used can not only meet nutrition needed for non-irrigated lotus root growth, additionally it is possible to supplement Selenium element, improves the Se content of non-irrigated lotus root stem tuber.
Not only bud ratio is high for the non-irrigated lotus root seedling that the present invention is obtained using tissue cultures, and stably, yield is high for variety and quality.
Further advantage of the invention, target and feature embody part by following explanation, and part will also be by this The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
<Embodiment 1>
The implantation methods of the selenium-rich drought lotus root of a kind of use tissue-cultured seedling, it is characterised in that comprise the following steps:
Irrigation and drainage water system is set up in a, the selection field that soil layer is deep, loosing soil is fertile as plantation field;
B, non-irrigated lotus root transplant first trimester, after 40 centimetres of plantation field deep ploughing, applying soil sterilants, to soil disinfection 5 days Afterwards, in mass ratio it is 1 to applying in plantation field by selenium mineral powder and metallurgical ground-slag:1 mineral powder 80kg/ mus for mixing, to soil After earth is sterilized 10 days, to 20kg/ mus of calcium and magnesium potassium soil conditioner or calcium potassium soil conditioner is applied in plantation field, 40 are deep ploughed again Centimetre, the previous moon is transplanted, after 1200kg/ mus of selenium-rich base fertilizer is applied in plantation field, deep plough 40 centimetres;
Wherein, the preparation method of the selenium-rich base fertilizer, comprises the following steps:
Step one, the root of water hyacinth is placed in the sodium selenite aqueous solution that mass fraction is 1%, daily sooner or later to water The sodium selenite aqueous solution that mass fraction is 45% is sprayed on cucurbit leaf, after 20 days, water hyacinth is gathered in and is shredded, after fermenting 2 days, Selenium-rich green manure is obtained, it is standby;
Step 2, well-rotted farmyard manure is placed under sunlight and be exposed to the sun after 2 days, crushed, dry farm manure is obtained, it is standby;
Step 3, by the stalk of 100 weight portions, the peanut meal of 100 weight portions, the sesame seed meal of 100 weight portions, 100 weight After the saccharomycete of the black soya bean dregs of rice, the Trichoderma of 10 weight portions and 10 weight portions of part is well mixed, the clear water of 300 weight portions is added, Stir, fermentation 7 days after, add 30 weight portions bamboo charcoal sponge, 300 weight portion the step of one in obtained selenium-rich green manure, The Mixed Microbes of obtained dry farm manure and 2 weight portions, stir in the step of 1200 weight portion two, ferment 15 days, obtain final product institute State selenium-rich base fertilizer;
Wherein, the Mixed Microbes are by silicate bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen-fixing bacteria, Verticillium lecanii, whiterot fungi and ferment Female bacterium is 5 in mass ratio:3:3:2:1:1:2 mix;
When c, non-irrigated lotus root tissue-cultured seedling grow 3-5 piece leaves, transplanted;
D, transplanting are first 7 days, after planting 20 centimetres of field deep ploughing, are exposed to the sun 2 days, and fine sand 100kg/ mus and wood chip are spread fertilizer over the fields afterwards After 80kg/ mus, the topsoil with plantation field is well mixed;
E, in plantation field dig plant hole, plant hole is deep 2 centimetres, and line-spacing is 70 centimetres, and spacing in the rows is 50 centimetres;
F, when lotus root seedling it is long to 10 centimetres when, every 7 days to plantation field in spray Selenium-rich nutrient solution, after lotus root seedling is emerged 30 days, to Plantation applies 50kg/ mus of selenium-rich fertilizer in field;
Wherein, the Selenium-rich nutrient solution is made up of the component of following parts by weight:
3 parts of potassium dihydrogen phosphate, 5 parts of potassium sulfate, 1 part of boric acid, 1 part of manganese chloride, 0.5 part of zinc sulfate, sodium selenite or selenic acid 800 parts of 3 parts of sodium, 2 parts of calcium superphosphate and water;
The selenium-rich fertilizer is made up of the component of following parts by weight:
2 parts of 12 parts of nitrogen-phosphorus-potassium compound fertilizer, 2 parts of sodium selenite, 0.2 part of glutamic acid, 0.2 part of tryptophan and boron fertilizer.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the metallurgical slag is blast furnace slag.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the method for tissue culture bag of the non-irrigated lotus root tissue-cultured seedling Include following steps:
Step one), by the stem tuber mass fraction of kind of lotus root be 70% alcohol disinfecting after, be put into sterilized water and soak 1 point Clock, then by the alcohol disinfecting that the stem apex part mass fraction of the stem tuber of kind of lotus root is 75%, then with aseptic water washing, repeats 3 After secondary, sterilized 5 minutes with liquor natrii hypochloritis that mass fraction is 5%, with aseptic water washing 3 times after, be put into sterilized water and soak Bubble 20 minutes, obtains explant;
Step 2), by step one) in the explant that obtains be put into the first culture medium in cultivate, prior to 25 DEG C, and completely cut off light It it is 23 DEG C in temperature after being cultivated 5 days under conditions of line, intensity of illumination is 1200lux, and light application time is under conditions of 8 hours/day Culture 15 days, obtains the first aseptic seedling;
Wherein, first culture medium includes:The 6-benzyladenine of MS, 2mg/L, the 2,4 dichloro benzene oxygen of 0.3mg/L The agar of acetic acid, the gibberellin of 0.3mg/L, 25g/L sucrose and 6g/L;
Step 3), by step 2) in the first aseptic seedling for obtaining be placed in the second culture medium, be 23 DEG C in cultivation temperature, Intensity of illumination is 1200lux, and light application time obtains the second aseptic seedling to be cultivated 15 days under conditions of 8 hours/day;
Wherein, second culture medium includes:The 6-benzyladenine of MS, 2mg/L, the methyl α-naphthyl acetate of 0.4mg/L, 0.5mg/ The agar of the TDZ, 25g/L sucrose and 6g/L of L;
Step 4), by step 3) in the second aseptic seedling for obtaining be placed in the 3rd culture medium, be 23 DEG C in cultivation temperature, Intensity of illumination is 1200lux, and light application time obtains the 3rd aseptic seedling to be cultivated 15 days under conditions of 8 hours/day;
Wherein, the 3rd culture medium includes:The 6-benzyladenine of 1/2MS, 1.5mg/L, the methyl α-naphthyl acetate of 0.4mg/L, The agar of the gibberellin, 25g/L sucrose and 6g/L of 0.3mg/L.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the method for tissue culture of the non-irrigated lotus root tissue-cultured seedling is also Comprise the following steps:
Step 5), whne step 4) in the 3rd aseptic seedling that the obtains piece leaf to 3-4 long when, hardening 4 days, to the 3rd aseptic seedling When hardening grows 3-5 piece leaves, you can transplanted.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, first culture medium, second culture medium Initial pH value with the 3rd culture medium is 5.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, also include:
G, the first tenday period of a month in April, in lotus root seedling gap green manuring crop in the ranks, after green manure crop harvests, green manure crop is buried In in plantation field.
<Embodiment 2>
The implantation methods of the selenium-rich drought lotus root of a kind of use tissue-cultured seedling, it is characterised in that comprise the following steps:
Irrigation and drainage water system is set up in a, the selection field that soil layer is deep, loosing soil is fertile as plantation field;
B, non-irrigated lotus root transplant first trimester, after 45 centimetres of plantation field deep ploughing, applying soil sterilants, to soil disinfection 6 days Afterwards, in mass ratio it is 1 to applying in plantation field by selenium mineral powder and metallurgical ground-slag:3 mineral powder 100kg/ mus for mixing, to soil After earth is sterilized 12 days, to 30kg/ mus of calcium and magnesium potassium soil conditioner or calcium potassium soil conditioner is applied in plantation field, 45 are deep ploughed again Centimetre, the previous moon is transplanted, after 1350kg/ mus of selenium-rich base fertilizer is applied in plantation field, deep plough 45 centimetres;
Wherein, the preparation method of the selenium-rich base fertilizer, comprises the following steps:
Step one, the root of water hyacinth is placed in the sodium selenite aqueous solution that mass fraction is 2%, daily sooner or later to water The sodium selenite aqueous solution that mass fraction is 48% is sprayed on cucurbit leaf, after 25 days, water hyacinth is gathered in and is shredded, after fermenting 3 days, Selenium-rich green manure is obtained, it is standby;
Step 2, well-rotted farmyard manure is placed under sunlight and be exposed to the sun after 3 days, crushed, dry farm manure is obtained, it is standby;
Step 3, by the stalk of 150 weight portions, the peanut meal of 150 weight portions, the sesame seed meal of 150 weight portions, 150 weight After the saccharomycete of the black soya bean dregs of rice, the Trichoderma of 15 weight portions and 15 weight portions of part is well mixed, the clear water of 450 weight portions is added, Stir, fermentation 10 days after, add 40 weight portions bamboo charcoal sponge, 400 weight portion the step of one in obtained selenium-rich green manure, The Mixed Microbes of obtained dry farm manure and 35 weight portions, stir in the step of 1350 weight portion two, ferment 18 days, obtain final product institute State selenium-rich base fertilizer;
Wherein, the Mixed Microbes are by silicate bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen-fixing bacteria, Verticillium lecanii, whiterot fungi and ferment Female bacterium is 5 in mass ratio:3:3:2:1:1:2 mix;
When c, non-irrigated lotus root tissue-cultured seedling grow 3-5 piece leaves, transplanted;
D, transplanting are first 8 days, after planting 25 centimetres of field deep ploughing, are exposed to the sun 3 days, and fine sand 110kg/ mus and wood chip are spread fertilizer over the fields afterwards After 90kg/ mus, the topsoil with plantation field is well mixed;
E, in plantation field dig plant hole, plant hole is deep 3 centimetres, and line-spacing is 135 centimetres, and spacing in the rows is 55 centimetres;
F, when lotus root seedling it is long to 12 centimetres when, every 8 days to plantation field in spray Selenium-rich nutrient solution, after lotus root seedling is emerged 32 days, to Plantation applies 50-80kg/ mus of selenium-rich fertilizer in field;
Wherein, the Selenium-rich nutrient solution is made up of the component of following parts by weight:
4 parts of potassium dihydrogen phosphate, 8 parts of potassium sulfate, 1.5 parts of boric acid, 1.5 parts of manganese chloride, 0.8 part of zinc sulfate, sodium selenite or 900 parts of 4 parts of sodium selenate, 4 parts of calcium superphosphate and water;
The selenium-rich fertilizer is made up of the component of following parts by weight:
2.5 parts of 13 parts of nitrogen-phosphorus-potassium compound fertilizer, 2.5 parts of sodium selenite, 0.25 part of glutamic acid, 0.25 part of tryptophan and boron fertilizer.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the metallurgical slag is vessel slag.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the method for tissue culture bag of the non-irrigated lotus root tissue-cultured seedling Include following steps:
Step one), by the stem tuber mass fraction of kind of lotus root be 70% alcohol disinfecting after, be put into sterilized water and soak 2 points Clock, then by the alcohol disinfecting that the stem apex part mass fraction of the stem tuber of kind of lotus root is 75%, then with aseptic water washing, repeats 4 After secondary, sterilized 8 minutes with liquor natrii hypochloritis that mass fraction is 12%, with aseptic water washing 4 times after, be put into sterilized water and soak Bubble 25 minutes, obtains explant;
Step 2), by step one) in the explant that obtains be put into the first culture medium in cultivate, prior to 28 DEG C, and completely cut off light It it is 25 DEG C in temperature after being cultivated 7 days under conditions of line, intensity of illumination is 1500lux, light application time is the bar in 8-10 hours/day Cultivated 15-20 days under part, obtain the first aseptic seedling;
Wherein, first culture medium includes:The 6-benzyladenine of MS, 2.5mg/L, the 2,4 dichloro benzene oxygen of 05mg/L The agar of acetic acid, the gibberellin of 0.5mg/L, 30g/L sucrose and 8g/L;
Step 3), by step 2) in the first aseptic seedling for obtaining be placed in the second culture medium, be 25 DEG C in cultivation temperature, Intensity of illumination is 1600lux, and light application time obtains the second aseptic seedling to be cultivated 18 days under conditions of 10 hours/day;
Wherein, second culture medium includes:The 6-benzyladenine of MS, 2.5mg/L, the methyl α-naphthyl acetate of 0.5mg/L, The agar of the TDZ, 30g/L sucrose and 8g/L of 1.2mg/L;
Step 4), by step 3) in the second aseptic seedling for obtaining be placed in the 3rd culture medium, be 25 DEG C in cultivation temperature, Intensity of illumination is 1600lux, and light application time obtains the 3rd aseptic seedling to be cultivated 18 days under conditions of 10 hours/day;
Wherein, the 3rd culture medium includes:The 6-benzyladenine of 1/2MS, 2.2mg/L, the methyl α-naphthyl acetate of 0.5mg/L, The agar of the gibberellin, 30g/L sucrose and 8g/L of 0.5mg/L.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the method for tissue culture of the non-irrigated lotus root tissue-cultured seedling is also Comprise the following steps:
Step 5), whne step 4) in the 3rd aseptic seedling that the obtains piece leaf to 3-4 long when, hardening 5 days, to the 3rd aseptic seedling When hardening grows 3-5 piece leaves, you can transplanted.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, first culture medium, second culture medium Initial pH value with the 3rd culture medium is 6.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, also include:
G, the first tenday period of a month in April, in lotus root seedling gap green manuring crop in the ranks, after green manure crop harvests, green manure crop is buried In in plantation field.
<Embodiment 3>
The implantation methods of the selenium-rich drought lotus root of a kind of use tissue-cultured seedling, it is characterised in that comprise the following steps:
Irrigation and drainage water system is set up in a, the selection field that soil layer is deep, loosing soil is fertile as plantation field;
B, non-irrigated lotus root transplant first trimester, after 50 centimetres of plantation field deep ploughing, applying soil sterilants, to soil disinfection 7 days Afterwards, in mass ratio it is 1 to applying in plantation field by selenium mineral powder and metallurgical ground-slag:5 mineral powder 120kg/ mus for mixing, to soil After earth is sterilized 15 days, to 40kg/ mus of calcium and magnesium potassium soil conditioner or calcium potassium soil conditioner is applied in plantation field, 50 are deep ploughed again Centimetre, the previous moon is transplanted, after 1500kg/ mus of selenium-rich base fertilizer is applied in plantation field, deep plough 50 centimetres;
Wherein, the preparation method of the selenium-rich base fertilizer, comprises the following steps:
Step one, the root of water hyacinth is placed in the sodium selenite aqueous solution that mass fraction is 3%, daily sooner or later to water The sodium selenite aqueous solution that mass fraction is 50% is sprayed on cucurbit leaf, after 30 days, water hyacinth is gathered in and is shredded, after fermenting 3 days, Selenium-rich green manure is obtained, it is standby;
Step 2, well-rotted farmyard manure is placed under sunlight and be exposed to the sun after 3 days, crushed, dry farm manure is obtained, it is standby;
Step 3, by the stalk of 200 weight portions, the peanut meal of 200 weight portions, the sesame seed meal of 200 weight portions, 200 weight After the saccharomycete of the black soya bean dregs of rice, the Trichoderma of 20 weight portions and 20 weight portions of part is well mixed, the clear water of 600 weight portions is added, Stir, fermentation 15 days after, add 50 weight portions bamboo charcoal sponge, 500 weight portion the step of one in obtained selenium-rich green manure, The Mixed Microbes of obtained dry farm manure and 50 weight portions, stir in the step of 1500 weight portion two, ferment 20 days, obtain final product institute State selenium-rich base fertilizer;
Wherein, the Mixed Microbes are by silicate bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen-fixing bacteria, Verticillium lecanii, whiterot fungi and ferment Female bacterium is 5 in mass ratio:3:3:2:1:1:2 mix;
When c, non-irrigated lotus root tissue-cultured seedling grow 3-5 piece leaves, transplanted;
D, transplanting are first 10 days, after planting 30 centimetres of field deep ploughing, are exposed to the sun 3 days, and fine sand 120kg/ mus and wood chip are spread fertilizer over the fields afterwards After 100kg/ mus, the topsoil with plantation field is well mixed;
E, in plantation field dig plant hole, plant hole is deep 4 centimetres, and line-spacing is 100 centimetres, and spacing in the rows is 60 centimetres;
F, when lotus root seedling it is long to 15 centimetres when, every 10 days to plantation field in spray Selenium-rich nutrient solution, after lotus root seedling is emerged 35 days, to Plantation applies 80kg/ mus of selenium-rich fertilizer in field;
Wherein, the Selenium-rich nutrient solution is made up of the component of following parts by weight:
5 parts of potassium dihydrogen phosphate, 10 parts of potassium sulfate, 2 parts of boric acid, 2 parts of manganese chloride, 1 part of zinc sulfate, sodium selenite or sodium selenate 1000 parts of 5 parts, 5 parts of calcium superphosphate and water;
The selenium-rich fertilizer is made up of the component of following parts by weight:
3 parts of 15 parts of nitrogen-phosphorus-potassium compound fertilizer, 3 parts of sodium selenite, 0.3 part of glutamic acid, 0.3 part of tryptophan and boron fertilizer.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the metallurgical slag is blast furnace slag, vessel slag and electricity Any one or a few in slag.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the method for tissue culture bag of the non-irrigated lotus root tissue-cultured seedling Include following steps:
Step one), by the stem tuber mass fraction of kind of lotus root be 70% alcohol disinfecting after, be put into sterilized water and soak 3 points Clock, then by the alcohol disinfecting that the stem apex part mass fraction of the stem tuber of kind of lotus root is 75%, then with aseptic water washing, repeats 5 After secondary, sterilized 10 minutes with liquor natrii hypochloritis that mass fraction is 20%, with aseptic water washing 5 times after, be put into sterilized water Immersion 30 minutes, obtains explant;
Step 2), by step one) in the explant that obtains be put into the first culture medium in cultivate, prior to 30 DEG C, and completely cut off light It it is 28 DEG C in temperature after being cultivated 8 days under conditions of line, intensity of illumination is 1800lux, and light application time is the condition of 10 hours/day Lower culture 20 days, obtains the first aseptic seedling;
Wherein, first culture medium includes:The 6-benzyladenine of MS, 3mg/L, the 2,4 dichloro benzene oxygen of 0.8mg/L The agar of acetic acid, the gibberellin of 0.8mg/L, 35g/L sucrose and 10g/L;
Step 3), by step 2) in the first aseptic seedling for obtaining be placed in the second culture medium, be 28 DEG C in cultivation temperature, Intensity of illumination is 2000lux, and light application time obtains the second aseptic seedling to be cultivated 20 days under conditions of 12 hours/day;
Wherein, second culture medium includes:The 6-benzyladenine of MS, 3mg/L, the methyl α-naphthyl acetate of 0.6mg/L, 2.0mg/ The agar of the TDZ, 35g/L sucrose and 6-10g/L of L;
Step 4), by step 3) in the second aseptic seedling for obtaining be placed in the 3rd culture medium, be 27 DEG C in cultivation temperature, Intensity of illumination is 2000lux, and light application time obtains the 3rd aseptic seedling to be cultivated 20 days under conditions of 12 hours/day;
Wherein, the 3rd culture medium includes:The 6-benzyladenine of 1/2MS, 3mg/L, the methyl α-naphthyl acetate of 0.6mg/L, The agar of the gibberellin, 35g/L sucrose and 10g/L of 0.8mg/L.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, the method for tissue culture of the non-irrigated lotus root tissue-cultured seedling is also Comprise the following steps:
Step 5), whne step 4) in the 3rd aseptic seedling that the obtains piece leaf to 3-4 long when, hardening 7 days, to the 3rd aseptic seedling When hardening grows 3-5 piece leaves, you can transplanted.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, first culture medium, second culture medium Initial pH value with the 3rd culture medium is 6.
In the implantation methods of the selenium-rich drought lotus root of described use tissue-cultured seedling, also include:
G, the first tenday period of a month in April, in lotus root seedling gap green manuring crop in the ranks, after green manure crop harvests, green manure crop is buried In in plantation field.
<Comparative example 1>
In stepb, after to soil disinfection 5 days, to metallurgical 80kg/ mus of ground-slag is applied in plantation field, to soil disinfection 10 After it, to applying 20kg/ mus of calcium and magnesium potassium soil conditioner or calcium potassium soil conditioner in plantation field, in remaining parameter and example 1 It is identical, process is also identical.
<Comparative example 2>
In step 2) in, by step one) in the explant that obtains be put into the first culture medium in cultivate, be 28 DEG C in temperature, Intensity of illumination is 1800lux, and light application time obtains the first aseptic seedling, remaining parameter to be cultivated 28 days under conditions of 10 hours/day Identical with embodiment 3, process is also identical.
The germination percentage of non-irrigated lotus root tuber quality, non-irrigated lotus root stem tuber Se content and tissue-cultured seedling that the every mu of plantation field of table 1 harvests every year
As shown in Table 1, to applying in plantation field by selenium mineral powder and metallurgical ground-slag in mass ratio it is 1 in embodiment 1:1 mixing Mineral powder 80kg/ mus, non-irrigated lotus root stem tuber Se content is significantly higher than in comparative example 1 to applying metallurgical ground-slag in plantation field 80kg/ mus;By step one in embodiment 3) in the explant that obtains be put into the first culture medium in cultivate, prior to 30 DEG C, and completely cut off It it is 28 DEG C in temperature after being cultivated 8 days under conditions of light, intensity of illumination is 1800lux, and light application time is the bar of 10 hours/day Cultivated 20 days under part, obtain the first aseptic seedling, the germination percentage of tissue-cultured seedling be significantly higher than in comparative example 2 by step one) in obtain Explant be put into the first culture medium and cultivate, be 28 DEG C in temperature, intensity of illumination is 1800lux, light application time is 10 hours/ Cultivated 28 days under conditions of it, obtain the first aseptic seedling.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in specification and implementation method With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited In specific details and shown here as the embodiment with description.

Claims (6)

1. a kind of use tissue-cultured seedling selenium-rich drought lotus root implantation methods, it is characterised in that comprise the following steps:
Irrigation and drainage water system is set up in a, the selection field that soil layer is deep, loosing soil is fertile as plantation field;
B, non-irrigated lotus root transplant first trimester, after 40-50 centimetres of plantation field deep ploughing, applying soil sterilants, to soil disinfection 5-7 days Afterwards, in mass ratio it is 1 to applying in plantation field by selenium mineral powder and metallurgical ground-slag:The mineral powder 80-120kg/ that 1-5 is mixed Mu, to soil disinfection 10-15 days after, to plantation field in applying calcium and magnesium potassium soil conditioner or calcium potassium soil conditioner 20-40kg/ Mu, deep ploughs 40-50 centimetres again, transplants the previous moon, after 1200-1500kg/ mus of selenium-rich base fertilizer is applied in plantation field, deep ploughing 40-50 centimetres;
Wherein, the preparation method of the selenium-rich base fertilizer, comprises the following steps:
Step one, the root of water hyacinth is placed in mass fraction be 1-3% sodium selenite aqueous solution in, daily sooner or later to water calabash It is the sodium selenite aqueous solution of 45-50% that mass fraction is sprayed on Lu Ye, after 20-30 days, gathers in water hyacinth and shreds, and ferment 2- After 3 days, selenium-rich green manure is obtained, it is standby;
Step 2, well-rotted farmyard manure is placed under sunlight and be exposed to the sun after 2-3 days, crushed, dry farm manure is obtained, it is standby;
Step 3, by the stalk of 100-200 weight portions, the peanut meal of 100-200 weight portions, the sesame seed meal of 100-200 weight portions, After the saccharomycete of the black soya bean dregs of rice, the Trichoderma of 10-20 weight portions and 10-20 weight portions of 100-200 weight portions is well mixed, add The clear water of 300-600 weight portions, stirs, and after fermenting 7-15 days, adds bamboo charcoal sponge, the 300-500 weights of 30-50 weight portions Amount part the step of one in obtained selenium-rich green manure, 1200-1500 weight portions the step of two in obtained dry farm manure and 20-50 weight The Mixed Microbes of part are measured, is stirred, fermented 15-20 days, obtain final product the selenium-rich base fertilizer;
Wherein, the Mixed Microbes are by silicate bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen-fixing bacteria, Verticillium lecanii, whiterot fungi and saccharomycete In mass ratio it is 5:3:3:2:1:1:2 mix;
When c, non-irrigated lotus root tissue-cultured seedling grow 3-5 piece leaves, transplanted;
7-10 days before d, transplanting, after 20-30 centimetres of field deep ploughing will be planted, it is exposed to the sun 2-3 days, fine sand 100-120kg/ mus is spread fertilizer over the fields afterwards After wood chip 80-100kg/ mus, the topsoil with plantation field is well mixed;
E, in plantation field dig plant hole, plant hole is deep 2-4 centimetres, and line-spacing is 70-100 centimetres, and spacing in the rows is 50-60 centimetres;
F, when lotus root seedling it is long to 10-15 centimetres when, per 7-10 days to plant field in spray Selenium-rich nutrient solution, lotus root seedling is emerged 30-35 days Afterwards, to plantation field in apply 50-80kg/ mus of selenium-rich fertilizer;
Wherein, the Selenium-rich nutrient solution is made up of the component of following parts by weight:
Potassium dihydrogen phosphate 3-5 parts, potassium sulfate 5-10 parts, boric acid 1-2 parts, manganese chloride 1-2 parts, zinc sulfate 0.5-1 parts, sodium selenite Or sodium selenate 3-5 parts, calcium superphosphate 2-5 parts and water 800-1000 parts;
The selenium-rich fertilizer is made up of the component of following parts by weight:
Nitrogen-phosphorus-potassium compound fertilizer 12-15 parts, sodium selenite 2-3 parts, glutamic acid 0.2-0.3 parts, tryptophan 0.2-0.3 parts and boron fertilizer 2- 3 parts.
2. implantation methods of the selenium-rich drought lotus root using tissue-cultured seedling as claimed in claim 1, it is characterised in that the metallurgical slag is Any one or a few in blast furnace slag, vessel slag and electroslag.
3. the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling are used as claimed in claim 1, it is characterised in that the non-irrigated lotus root tissue culture The method for tissue culture of seedling is comprised the following steps:
Step one), by the stem tuber mass fraction of kind of lotus root be 70% alcohol disinfecting after, be put into sterilized water and soak 1-3 minutes, Then by the alcohol disinfecting that the stem apex part mass fraction of the stem tuber of kind of lotus root is 75%, then with aseptic water washing, repeat 3-5 times Afterwards, sterilized 5-10 minutes with liquor natrii hypochloritis that mass fraction is 5-20%, with aseptic water washing 3-5 times after, be put into aseptic Soaked 20-30 minutes in water, obtain explant;
Step 2), by step one) in the explant that obtains be put into the first culture medium in cultivate, prior to 25-30 DEG C, and completely cut off light It it is 23-28 DEG C in temperature after being cultivated 5-8 days under conditions of line, intensity of illumination is 1200-1800lux, light application time is that 8-10 is small When/day under conditions of cultivate 15-20 days, obtain the first aseptic seedling;
Wherein, first culture medium includes:The 6-benzyladenine of MS, 2-3mg/L, the 2,4 dichloro benzene of 0.3-0.8mg/L The agar of fluoroacetic acid, the gibberellin of 0.3-0.8mg/L, 25-35g/L sucrose and 6-10g/L;
Step 3), by step 2) in the first aseptic seedling for obtaining be placed in the second culture medium, be 23-28 DEG C in cultivation temperature, Intensity of illumination is 1200-2000lux, and light application time obtains second aseptic to be cultivated 15-20 days under conditions of 8-12 hours/day Seedling;
Wherein, second culture medium includes:The 6-benzyladenine of MS, 2-3mg/L, the methyl α-naphthyl acetate of 0.4-0.6mg/L, 0.5- The agar of the TDZ, 25-35g/L sucrose and 6-10g/L of 2.0mg/L;
Step 4), by step 3) in the second aseptic seedling for obtaining be placed in the 3rd culture medium, be 23-27 DEG C in cultivation temperature, Intensity of illumination is 1200-2000lux, and light application time obtains the 3rd aseptic to be cultivated 15-20 days under conditions of 8-12 hours/day Seedling;
Wherein, the 3rd culture medium includes:The 6-benzyladenine of 1/2MS, 1.5-3mg/L, the naphthalene second of 0.4-0.6mg/L The agar of acid, the gibberellin of 0.3-0.8mg/L, 25-35g/L sucrose and 6-10g/L.
4. the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling are used as claimed in claim 3, it is characterised in that the non-irrigated lotus root tissue culture The method for tissue culture of seedling is further comprising the steps of:
Step 5), whne step 4) in the 3rd aseptic seedling that the obtains piece leaf to 3-4 long when, hardening 4-7 days, to the refining of the 3rd aseptic seedling When seedling grows 3-5 piece leaves, you can transplanted.
5. the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling are used as claimed in claim 4, it is characterised in that first culture The initial pH value of base, second culture medium and the 3rd culture medium is 5-6.
6. the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling are used as claimed in claim 1, it is characterised in that also included:
G, the first tenday period of a month in April, in lotus root seedling gap green manuring crop in the ranks, after green manure crop harvests, green manure crop is embedded in kind Plant in field.
CN201611030422.9A 2016-11-16 2016-11-16 Use the implantation methods of the selenium-rich drought lotus root of tissue-cultured seedling Pending CN106717777A (en)

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CN107915516A (en) * 2017-11-14 2018-04-17 蒋钦辉 Use the selenium-rich sweet potato implantation methods of tissue-cultured seedling
CN109392573A (en) * 2018-11-09 2019-03-01 梁斌 The cultural method of selenium-rich mango

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CN109392573A (en) * 2018-11-09 2019-03-01 梁斌 The cultural method of selenium-rich mango

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Application publication date: 20170531