CN107912589A - Containing probiotics and enteron aisle and the pressed candy of immunity and preparation method thereof can be improved - Google Patents
Containing probiotics and enteron aisle and the pressed candy of immunity and preparation method thereof can be improved Download PDFInfo
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- CN107912589A CN107912589A CN201711394771.3A CN201711394771A CN107912589A CN 107912589 A CN107912589 A CN 107912589A CN 201711394771 A CN201711394771 A CN 201711394771A CN 107912589 A CN107912589 A CN 107912589A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 36
- 230000036039 immunity Effects 0.000 title abstract description 10
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- 108091005804 Peptidases Proteins 0.000 claims description 48
- 239000004365 Protease Substances 0.000 claims description 48
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 48
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- 235000019710 soybean protein Nutrition 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 239000000413 hydrolysate Substances 0.000 claims description 34
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- 102000004169 proteins and genes Human genes 0.000 claims description 32
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- 241000186604 Lactobacillus reuteri Species 0.000 claims description 25
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 25
- 239000000706 filtrate Substances 0.000 claims description 24
- 229940001882 lactobacillus reuteri Drugs 0.000 claims description 24
- 238000002156 mixing Methods 0.000 claims description 24
- 239000006228 supernatant Substances 0.000 claims description 24
- 241000186605 Lactobacillus paracasei Species 0.000 claims description 23
- 238000005119 centrifugation Methods 0.000 claims description 20
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- 235000019634 flavors Nutrition 0.000 claims description 19
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- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims description 17
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- 239000003513 alkali Substances 0.000 claims description 16
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 11
- 239000000284 extract Substances 0.000 claims description 11
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/364—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
- A23G3/366—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins containing microorganisms, enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/42—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds characterised by the carbohydrates used, e.g. polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/44—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
Abstract
Containing probiotics and enteron aisle and the pressed candy of immunity and preparation method thereof can be improved the present invention relates to a kind of, the raw material components of pressed candy by weight, including：5~10 parts by weight of probiotics, 3~7 parts by weight of 10~15 parts by weight of galactooligosaccharide, 5~8 parts by weight of oligofructose and glucose.Pressed candy provided by the invention, selecting various nutrients, prebiotics and probiotics, the pressed candy being prepared is full of nutrition, convenient as raw material components, suitable children eat, and with the effect for improving function of intestinal canal, strengthen immunity and enhancing antiallergy ability.
Description
Technical field
The present invention relates to food technology field, and in particular to a kind of containing probiotics and to improve the tabletting of enteron aisle and immunity
Candy and preparation method thereof.
Background technology
Candy is a kind of common food, and the species of candy is also varied.Traditional candy categories of food can only meet
People supplement the nutrients and the demand of food variation aspect, usually only focus on the requirement of color etc., it includes
Nutriment often only focuses on the needs of human body supplement energy.As the improvement of people's living standards, people increasingly focus on drinking
The influence to own bodies health is eaten, therefore, the candy categories of food for having healthcare function and being adapted to children to eat becomes candy
The new trend of based food development.
The content of the invention
For in the prior art the defects of, present invention aims at provide a kind of containing probiotics and to improve enteron aisle and immune
Pressed candy of power and preparation method thereof, to select various nutrients, prebiotics and probiotics to make to be prepared into as raw material components
The pressed candy arrived is full of nutrition, convenient, and with improvement function of intestinal canal, strengthen immunity and enhancing antiallergy ability
Effect.
To achieve the above object, technical solution provided by the invention is：
The present invention provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 5
~10 parts by weight, 3~7 parts by weight of 10~15 parts by weight of galactooligosaccharide, 5~8 parts by weight of oligofructose and glucose.
Probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri.
Bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri CFU ratios for (0.9~
1.1):(0.4~0.6):(1.0~1.3):(0.7~0.9).
Preferably, the raw material components of pressed candy further include：4~6 parts by weight of isomalto-oligosaccharide, 3~5 weight of walnut peptide
Part, 2~4 parts by weight of pea peptide, 1.5~2.0 parts by weight of soybean protein isolate, 0.5~1.0 parts by weight of fish oil and milk alkalescence egg
White 1~2 parts by weight.
The preparation method of walnut peptide includes step：Grease is removed after walnut is crushed, obtains walnut powder；Add in walnut powder
Enter water, then carry out defibrination, filter, collect filtrate, obtain walnut protein slurry；The pH value that walnut protein is starched is adjusted to 7.0~
8.0, trypsase is then added, carries out first time enzymolysis；Then flavor protease is added, second is carried out and digests；By second
Mixture inactivation after secondary enzymolysis, then filters, collects filtrate；Filtrate is spray-dried, obtains walnut peptide.
Preferably, the ratio of walnut powder and water is (10~12):100；The addition of trypsase is 0.3~0.4g/
100mL walnut proteins are starched, and the temperature of enzymolysis is 46~50 DEG C for the first time, and the time of enzymolysis is 45~50min for the first time；Flavor egg
The addition of white enzyme is 1.6~1.8g/100mL walnut proteins slurry, and the temperature of second of enzymolysis is 50~52 DEG C, second of enzymolysis
Time be 1~1.5h；The temperature of inactivation is 80~85 DEG C, and the time of inactivation is 25~30min.
The preparation method of soybean protein isolate includes step：Defatted soybean meal powder and water are mixed, heated in alkaline conditions
It is 70~80 DEG C to temperature, extracts preset time, obtains extraction mixture；Alkali protease is added in mixture is extracted to carry out
Enzymolysis, obtains soybean protease hydrolysate；Soybean protease hydrolysate is centrifuged, collects hydrolysate of soybean protein supernatant；By soybean egg
White enzymolysis supernatant tune pH value is to 4.2~4.4, the albumen in centrifugation hydrolysate of soybean protein supernatant, by gained precipitation with
Water mixes, and is freeze-dried after pH value is adjusted to neutrality, obtains soybean protein isolate.
Preferably, the mass ratio of defatted soybean meal powder and water is (22~25):100, the pH value of alkaline condition is 8.2~8.4,
The time of extraction is 40~50min；The addition of alkali protease extracts mixture for 0.35~0.45g/100mL, enzymolysis
Time is 2~3h；Soybean protease hydrolysate is centrifuged, is that 7000~8000r/min centrifuges 15~18min at 20~30 DEG C；From
Heart precipitation is that 7000~8000r/min centrifuges 23~25min at 20~30 DEG C；Gained precipitate with the mass ratio of water for (2.5~
3.0):1。
Present invention also offers the preparation method of the pressed candy comprising probiotics, including step：By in addition to probiotics
Other raw material components mix, and are then sprayed into the ethanol water that volume fraction is 50%~75%, obtain mixed slurry；Will mixing
Slurry is pelletized using wet granulator, is then dried, and obtains particle；Probiotics is added in the grain, is adopted after mixing
With rushing rotary tablet machine carries out tabletting more, obtain including the pressed candy of probiotics.
Preferably, the ratio of the quality of ethanol water and all raw material components gross masses is 1:(5.4~5.6)；
When mixing all raw material components, the step of adding colouring agent, flavor enhancement and binding agent is further included.
Technical solution provided by the invention, has following beneficial effect：(1) pressure provided by the invention for including probiotics
Piece candy, after children take orally, having improves function of intestinal canal, strengthen immunity and the effect for strengthening antiallergy ability；(2) it is of the invention
The pressed candy for including probiotics provided, comprising various nutrients, prebiotics and probiotics, each raw material coordinates science, improves
Function of intestinal canal, strengthen immunity and the significant effect for strengthening antiallergy ability, it is quick, and safety is without side-effects；(3) it is of the invention
The preparation method of the pressed candy comprising probiotics provided is simple, and production cost is low, has good commercial promise.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Embodiment
Below in conjunction with the embodiment of the present invention, technical scheme is clearly and completely described.Implement below
Example is only used for clearly illustrating technical scheme, therefore be intended only as example, and cannot limit this hair with this
Bright protection domain.
Experimental method in following embodiments, is conventional method unless otherwise specified.Examination used in following embodiments
Material is tested, is to be commercially available from regular shops unless otherwise specified.Quantitative test in following embodiments, is respectively provided with three
Secondary to repeat to test, data are the average value or mean+SD of three repeated experiments.The fish oil that the embodiment of the present invention uses,
English name is Fats and Glyceridic oils, and No. CAS is 8016-13-5.The bifidobacterium lactis that the present invention uses for
Bifidobacterium lactis BB-115, Lactobacillus rhamnosus are Lactobacillus rhamnosus CT-53, secondary
Lactobacillus casei is Lactobacillus paracasei GL-156, lactobacillus reuteri Lactobacillus
Reuteri GL-104, buy from Fenghua biotech inc.The trypsase that the present invention uses is tryptose
Enzyme Trypsin, CAS No.9002-07-7, flavor protease are compound fertilizer production, and CAS numbering 1786400-23-4, are purchased
Buy from hundred taste bio tech ltd of Hebei, the CAS numbering 9014-0-1 of alkali protease, buy from the suitable sky in Jiangsu and add biology
Science and Technology Ltd..
The present invention provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 5~
10 parts by weight, 3~7 parts by weight of 10~15 parts by weight of galactooligosaccharide, 5~8 parts by weight of oligofructose and glucose.
Preferably, raw material components further include：4~6 parts by weight of isomalto-oligosaccharide, 3~5 parts by weight of walnut peptide, pea peptide 2
~4 parts by weight, 1~2 weight of 1.5~2.0 parts by weight of soybean protein isolate, 0.5~1.0 parts by weight of fish oil and milk basic protein
Part.
Wherein, probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri, breast
Bifidobacterium, Lactobacillus rhamnosus, the CFU ratios of lactobacillus paracasei and lactobacillus reuteri are (0.9~1.1):(0.4~
0.6):(1.0~1.3):(0.7~0.9).
The preparation method of walnut peptide includes step：
Grease is removed after walnut is crushed, obtains walnut powder；
Add water in walnut powder, the ratio of walnut powder and water is (10~12):100, defibrination is then carried out, is filtered, is received
Collect filtrate, obtain walnut protein slurry；
The pH value that walnut protein is starched is adjusted to 7.0~8.0, then adds trypsase, the addition of trypsase is
0.3~0.4g/100mL walnut proteins are starched, and carry out first time enzymolysis, 46~50 DEG C of 45~50min of enzymolysis；Then flavor egg is added
White enzyme, the addition of flavor protease are starched for 1.6~1.8g/100mL walnut proteins, are carried out second and are digested, 50~52 DEG C of enzymes
Solve 1~1.5h；
80~85 DEG C of 25~30min of inactivation of mixture after second is digested, then filter, collect filtrate；By filtrate
It is spray-dried, obtains walnut peptide.
The preparation method of soybean protein isolate includes step：
By defatted soybean meal powder and water with (22~25):100 mass ratio mixing, in the case where alkaline condition pH value is 8.2~8.4
Temperature is heated to as 70~80 DEG C, extracts 40~50min, obtains extraction mixture；Basic protein is added in mixture is extracted
Enzyme, the addition of alkali protease extract mixture for 0.35~0.45g/100mL, digest 2~3h, obtain hydrolysate of soybean protein
Liquid；
By 20~30 DEG C of soybean protease hydrolysate, 7000~8000r/min centrifuges 15~18min, collects soybean protein enzyme
Solve supernatant；
By hydrolysate of soybean protein supernatant tune pH value to 4.2~4.4, the egg in centrifugation hydrolysate of soybean protein supernatant
In vain, centrifugation is to centrifuge 23~25min in 20~30 DEG C of progress, 7000~8000r/min, by gained precipitation with water with (2.5
~3.0):1 mass ratio mixing, is freeze-dried after pH value is adjusted to neutrality, obtains soybean protein isolate.
In addition, present invention also offers the preparation method of the above-mentioned pressed candy comprising probiotics, include the following steps：
Other raw material components in addition to probiotics are mixed, it is water-soluble to be then sprayed into the ethanol that volume fraction is 50%~75%
The ratio of liquid, the quality of ethanol water and all raw material components gross masses is 1:(5.4~5.6), obtain mixed slurry；
Mixed slurry wet granulator is pelletized, is then dried, obtains particle；
Probiotics is added in the grain, is carried out tabletting using the rotary tablet machines that rush after mixing more, is obtained comprising benefit
The pressed candy of raw bacterium.
Containing probiotics and enteron aisle and the pressed candy of immunity can be improved to provided by the invention with reference to specific embodiment
Fruit and preparation method thereof is described further.
Embodiment one
The present embodiment provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 8
Parts by weight, 5 parts by weight of 12 parts by weight of galactooligosaccharide, 7 parts by weight of oligofructose and glucose.
Wherein, probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri, breast
Bifidobacterium, Lactobacillus rhamnosus, the CFU ratios of lactobacillus paracasei and lactobacillus reuteri are 1.0:0.5:1.2:0.8.
By above-mentioned raw material, using preparation method provided by the invention, the pressed candy for including probiotics is prepared：
Other raw material components in addition to probiotics are mixed, are then sprayed into the ethanol water that volume fraction is 65%, second
The ratio of the quality of alcohol solution and all raw material components gross masses is 1:5.5 obtain mixed slurry；
Mixed slurry is pelletized using wet granulator, then dries, obtains particle；
Probiotics is added in the grain, is carried out tabletting using the rotary tablet machines that rush after mixing more, is obtained comprising benefit
The pressed candy of raw bacterium.
Embodiment two
The present embodiment provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 5
Parts by weight, 3 parts by weight of 15 parts by weight of galactooligosaccharide, 5 parts by weight of oligofructose and glucose.
Wherein, probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri, breast
Bifidobacterium, Lactobacillus rhamnosus, the CFU ratios of lactobacillus paracasei and lactobacillus reuteri are 0.9:0.4:1.0:0.7.
By above-mentioned raw material, using preparation method provided by the invention, the pressed candy for including probiotics is prepared：
Other raw material components in addition to probiotics are mixed, are then sprayed into the ethanol water that volume fraction is 50%, second
The ratio of the quality of alcohol solution and all raw material components gross masses is 1:5.4, obtain mixed slurry；
Mixed slurry is pelletized using wet granulator, then dries, obtains particle；
Probiotics is added in the grain, is carried out tabletting using the rotary tablet machines that rush after mixing more, is obtained comprising benefit
The pressed candy of raw bacterium.
Embodiment three
The present embodiment provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 10
Parts by weight, 2 parts by weight of 10 parts by weight of galactooligosaccharide, 8 parts by weight of oligofructose and 7 parts by weight of glucose.
Wherein, probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri, breast
Bifidobacterium, Lactobacillus rhamnosus, the CFU ratios of lactobacillus paracasei and lactobacillus reuteri are 1.1:0.6:1.3:0.9.
By above-mentioned raw material, using preparation method provided by the invention, the pressed candy for including probiotics is prepared：
Other raw material components in addition to probiotics are mixed, are then sprayed into the ethanol water that volume fraction is 75%, second
The ratio of the quality of alcohol solution and all raw material components gross masses is 1:5.6, obtain mixed slurry；
Mixed slurry is pelletized using wet granulator, then dries, obtains particle；
Probiotics is added in the grain, is carried out tabletting using the rotary tablet machines that rush after mixing more, is obtained comprising benefit
The pressed candy of raw bacterium.
Example IV
The present embodiment provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 8
Parts by weight, 12 parts by weight of galactooligosaccharide, 7 parts by weight of oligofructose, 5 parts by weight of glucose, 5 parts by weight of isomalto-oligosaccharide,
4 parts by weight of walnut peptide, 3 parts by weight of pea peptide, 1.8 parts by weight of soybean protein isolate, 0.8 parts by weight of fish oil and milk basic protein
1.5 parts by weight.
Wherein, probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri, breast
Bifidobacterium, Lactobacillus rhamnosus, the CFU ratios of lactobacillus paracasei and lactobacillus reuteri are 1.0:0.5:1.2:0.8.
The preparation method of walnut peptide includes step：
Grease is removed after walnut is crushed, obtains walnut powder；
Add water in walnut powder, the ratio of walnut powder and water is 11:100, defibrination is then carried out, is filtered, collects filtrate,
Obtain walnut protein slurry；
The pH value that walnut protein is starched is adjusted to 7.5, then adds trypsase, the addition of trypsase is 0.35g/
100mL walnut proteins are starched, and carry out first time enzymolysis, 48 DEG C of enzymolysis 47min；Then flavor protease is added, flavor protease
Addition is starched for 1.7g/100mL walnut proteins, is carried out second and is digested, 51 DEG C of enzymolysis 1.2h；
83 DEG C of inactivation 28min of mixture after second is digested, then filter, collect filtrate；Filtrate is sprayed
It is dry, obtain walnut peptide.
The preparation method of soybean protein isolate includes step：
By defatted soybean meal powder and water with 24:100 mass ratio mixing, temperature is heated under being 8.3 in alkaline condition pH value
For 75 DEG C, 45min is extracted, obtains extraction mixture；Alkali protease, the addition of alkali protease are added in mixture is extracted
Measure and extract mixture for 0.40g/100mL, digest 2.5h, obtain soybean protease hydrolysate；
By 25 DEG C of soybean protease hydrolysate, 7500r/min centrifugation 17min, collect hydrolysate of soybean protein supernatant；
By hydrolysate of soybean protein supernatant tune pH value to 4.3, the albumen in centrifugation hydrolysate of soybean protein supernatant, from
Heart precipitation is in 25 DEG C of progress, 7500r/min centrifugation 24min, by gained precipitation with water with 2.8:1 mass ratio mixing, by pH
Value is freeze-dried after being adjusted to neutrality, obtains soybean protein isolate.
By above-mentioned raw material, using preparation method provided by the invention, the pressed candy for including probiotics is prepared：
Other raw material components in addition to probiotics are mixed, are then sprayed into the ethanol water that volume fraction is 65%, second
The ratio of the quality of alcohol solution and all raw material components gross masses is 1:5.5, obtain mixed slurry；
Mixed slurry is pelletized using wet granulator, then dries, obtains particle；
Probiotics is added in the grain, is carried out tabletting using the rotary tablet machines that rush after mixing more, is obtained comprising benefit
The pressed candy of raw bacterium.
Embodiment five
The present embodiment provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 5
Parts by weight, 15 parts by weight of galactooligosaccharide, 5 parts by weight of oligofructose, 3 parts by weight of glucose, 4 parts by weight of isomalto-oligosaccharide,
5 parts by weight of walnut peptide, 2 parts by weight of pea peptide, 2.0 parts by weight of soybean protein isolate, 0.5 parts by weight of fish oil and milk basic protein
2 parts by weight.
Wherein, probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri, breast
Bifidobacterium, Lactobacillus rhamnosus, the CFU ratios of lactobacillus paracasei and lactobacillus reuteri are 0.9:0.4:1.0:0.7.
The preparation method of walnut peptide includes step：
Grease is removed after walnut is crushed, obtains walnut powder；
Add water in walnut powder, the ratio of walnut powder and water is 10:100, defibrination is then carried out, is filtered, collects filtrate,
Obtain walnut protein slurry；
The pH value that walnut protein is starched is adjusted to 7.0, then adds trypsase, the addition of trypsase is 0.3g/
100mL walnut proteins are starched, and carry out first time enzymolysis, 46 DEG C of enzymolysis 45min；Then flavor protease is added, flavor protease
Addition is starched for 1.6g/100mL walnut proteins, is carried out second and is digested, 50 DEG C of enzymolysis 1h；
80 DEG C of inactivation 25min of mixture after second is digested, then filter, collect filtrate；Filtrate is sprayed
It is dry, obtain walnut peptide.
The preparation method of soybean protein isolate includes step：
By defatted soybean meal powder and water with 22:100 mass ratio mixing, temperature is heated under being 8.2 in alkaline condition pH value
For 70 DEG C, 40min is extracted, obtains extraction mixture；Alkali protease, the addition of alkali protease are added in mixture is extracted
Measure and extract mixture for 0.35g/100mL, digest 2h, obtain soybean protease hydrolysate；
By 20 DEG C of soybean protease hydrolysate, 7000r/min centrifugation 15min, collect hydrolysate of soybean protein supernatant；
By hydrolysate of soybean protein supernatant tune pH value to 4.2, the albumen in centrifugation hydrolysate of soybean protein supernatant, from
Heart precipitation is in 20 DEG C of progress, 7000r/min centrifugation 23min, by gained precipitation with water with 2.5:1 mass ratio mixing, by pH
Value is freeze-dried after being adjusted to neutrality, obtains soybean protein isolate.
By above-mentioned raw material, using preparation method provided by the invention, the pressed candy for including probiotics is prepared：
Other raw material components in addition to probiotics are mixed, are then sprayed into the ethanol water that volume fraction is 50%, second
The ratio of the quality of alcohol solution and all raw material components gross masses is 1:5.4, obtain mixed slurry；
Mixed slurry is pelletized using wet granulator, then dries, obtains particle；
Probiotics is added in the grain, is carried out tabletting using the rotary tablet machines that rush after mixing more, is obtained comprising benefit
The pressed candy of raw bacterium.
Embodiment six
The present embodiment provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 10
Parts by weight, 10 parts by weight of galactooligosaccharide, 8 parts by weight of oligofructose, 7 parts by weight of glucose, 6 parts by weight of isomalto-oligosaccharide,
3 parts by weight of walnut peptide, 4 parts by weight of pea peptide, 1.5 parts by weight of soybean protein isolate, 1.0 parts by weight of fish oil and milk basic protein
1 parts by weight.
Wherein, probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri, breast
Bifidobacterium, Lactobacillus rhamnosus, the CFU ratios of lactobacillus paracasei and lactobacillus reuteri are 1.1:0.6:1.3:0.9.
The preparation method of walnut peptide includes step：
Grease is removed after walnut is crushed, obtains walnut powder；
Add water in walnut powder, the ratio of walnut powder and water is 12:100, defibrination is then carried out, is filtered, collects filtrate,
Obtain walnut protein slurry；
The pH value that walnut protein is starched is adjusted to 8.0, then adds trypsase, the addition of trypsase is 0.4g/
100mL walnut proteins are starched, and carry out first time enzymolysis, 50 DEG C of enzymolysis 50min；Then flavor protease is added, flavor protease
Addition is starched for 1.8g/100mL walnut proteins, is carried out second and is digested, 52 DEG C of enzymolysis 1.5h；
85 DEG C of inactivation 30min of mixture after second is digested, then filter, collect filtrate；Filtrate is sprayed
It is dry, obtain walnut peptide.
The preparation method of soybean protein isolate includes step：
By defatted soybean meal powder and water with 25:100 mass ratio mixing, temperature is heated under being 8.4 in alkaline condition pH value
For 80 DEG C, 50min is extracted, obtains extraction mixture；Alkali protease, the addition of alkali protease are added in mixture is extracted
Measure and extract mixture for 0.45g/100mL, digest 3h, obtain soybean protease hydrolysate；
By 30 DEG C of soybean protease hydrolysate, 8000r/min centrifugation 18min, collect hydrolysate of soybean protein supernatant；
By hydrolysate of soybean protein supernatant tune pH value to 4.4, the albumen in centrifugation hydrolysate of soybean protein supernatant, from
Heart precipitation is in 30 DEG C of progress, 8000r/min centrifugation 25min, by gained precipitation with water with 3.0:1 mass ratio mixing, by pH
Value is freeze-dried after being adjusted to neutrality, obtains soybean protein isolate.
By above-mentioned raw material, using preparation method provided by the invention, the pressed candy for including probiotics is prepared：
Other raw material components in addition to probiotics are mixed, are then sprayed into the ethanol water that volume fraction is 75%, second
The ratio of the quality of alcohol solution and all raw material components gross masses is 1:5.6, obtain mixed slurry；
Mixed slurry is pelletized using wet granulator, then dries, obtains particle；
Probiotics is added in the grain, is carried out tabletting using the rotary tablet machines that rush after mixing more, is obtained comprising benefit
The pressed candy of raw bacterium.
Comparative example one
This comparative example provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 8
Parts by weight, 12 parts by weight of galactooligosaccharide, 7 parts by weight of oligofructose, 5 parts by weight of glucose, 5 parts by weight of isomalto-oligosaccharide,
3 parts by weight of pea peptide, 1.5 parts by weight of 1.8 parts by weight of soybean protein isolate, 0.8 parts by weight of fish oil and milk basic protein.
Wherein, probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri, breast
Bifidobacterium, Lactobacillus rhamnosus, the CFU ratios of lactobacillus paracasei and lactobacillus reuteri are 1.0:0.5:1.2:0.8.
The preparation method of soybean protein isolate includes step：
By defatted soybean meal powder and water with 24:100 mass ratio mixing, temperature is heated under being 8.3 in alkaline condition pH value
For 75 DEG C, 45min is extracted, obtains extraction mixture；Alkali protease, the addition of alkali protease are added in mixture is extracted
Measure and extract mixture for 0.40g/100mL, digest 2.5h, obtain soybean protease hydrolysate；
By 25 DEG C of soybean protease hydrolysate, 7500r/min centrifugation 17min, collect hydrolysate of soybean protein supernatant；
By hydrolysate of soybean protein supernatant tune pH value to 4.3, the albumen in centrifugation hydrolysate of soybean protein supernatant, from
Heart precipitation is in 25 DEG C of progress, 7500r/min centrifugation 24min, by gained precipitation with water with 2.8:1 mass ratio mixing, by pH
Value is freeze-dried after being adjusted to neutrality, obtains soybean protein isolate.
The same example IV of preparation method.
Comparative example two
This comparative example provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 8
Parts by weight, 12 parts by weight of galactooligosaccharide, 7 parts by weight of oligofructose, 5 parts by weight of glucose, 5 parts by weight of isomalto-oligosaccharide,
4 parts by weight of walnut peptide, 1.5 parts by weight of 3 parts by weight of pea peptide, 0.8 parts by weight of fish oil and milk basic protein.
Wherein, probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri, breast
Bifidobacterium, Lactobacillus rhamnosus, the CFU ratios of lactobacillus paracasei and lactobacillus reuteri are 1.0:0.5:1.2:0.8.
The preparation method of walnut peptide includes step：
Grease is removed after walnut is crushed, obtains walnut powder；
Add water in walnut powder, the ratio of walnut powder and water is 11:100, defibrination is then carried out, is filtered, collects filtrate,
Obtain walnut protein slurry；
The pH value that walnut protein is starched is adjusted to 7.5, then adds trypsase, the addition of trypsase is 0.35g/
100mL walnut proteins are starched, and carry out first time enzymolysis, 48 DEG C of enzymolysis 47min；Then flavor protease is added, flavor protease
Addition is starched for 1.7g/100mL walnut proteins, is carried out second and is digested, 51 DEG C of enzymolysis 1.2h；
83 DEG C of inactivation 28min of mixture after second is digested, then filter, collect filtrate；Filtrate is sprayed
It is dry, obtain walnut peptide.
The same example IV of preparation method.
Comparative example three
This comparative example provides a kind of pressed candy for including probiotics, raw material components by weight, including：Probiotics 8
Parts by weight, 12 parts by weight of galactooligosaccharide, 7 parts by weight of oligofructose, 5 parts by weight of glucose, 5 parts by weight of isomalto-oligosaccharide,
4 parts by weight of walnut peptide, 3 parts by weight of pea peptide, 1.8 parts by weight of soybean protein isolate, 0.8 parts by weight of fish oil and milk basic protein
1.5 parts by weight.
Wherein, probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus and lactobacillus reuteri, bifidobacterium lactis, sandlwood
The CFU ratios of sugared lactobacillus and lactobacillus reuteri are 1.0:0.5:0.8.
The preparation method of walnut peptide includes step：
Grease is removed after walnut is crushed, obtains walnut powder；
Add water in walnut powder, the ratio of walnut powder and water is 11:100, defibrination is then carried out, is filtered, collects filtrate,
Obtain walnut protein slurry；
The pH value that walnut protein is starched is adjusted to 7.5, then adds trypsase, the addition of trypsase is 0.35g/
100mL walnut proteins are starched, and carry out first time enzymolysis, 48 DEG C of enzymolysis 47min；Then flavor protease is added, flavor protease
Addition is starched for 1.7g/100mL walnut proteins, is carried out second and is digested, 51 DEG C of enzymolysis 1.2h；
83 DEG C of inactivation 28min of mixture after second is digested, then filter, collect filtrate；Filtrate is sprayed
It is dry, obtain walnut peptide.
The preparation method of soybean protein isolate includes step：
By defatted soybean meal powder and water with 24:100 mass ratio mixing, temperature is heated under being 8.3 in alkaline condition pH value
For 75 DEG C, 45min is extracted, obtains extraction mixture；Alkali protease, the addition of alkali protease are added in mixture is extracted
Measure and extract mixture for 0.40g/100mL, digest 2.5h, obtain soybean protease hydrolysate；
By 25 DEG C of soybean protease hydrolysate, 7500r/min centrifugation 17min, collect hydrolysate of soybean protein supernatant；
By hydrolysate of soybean protein supernatant tune pH value to 4.3, the albumen in centrifugation hydrolysate of soybean protein supernatant, from
Heart precipitation is in 25 DEG C of progress, 7500r/min centrifugation 24min, by gained precipitation with water with 2.8:1 mass ratio mixing, by pH
Value is freeze-dried after being adjusted to neutrality, obtains soybean protein isolate.
The same example IV of preparation method.
The pressed candy for including probiotics that the embodiment of the present invention one to embodiment six is prepared, is tried by function assessment
Test and carry out system evaluation its effect, and using the pressed candy comprising probiotics that comparative example one to comparative example three is prepared as pair
According to.
1st, the repelling and killing efficacy to the parasite for causing diarrhea
Test method：It is 18~22g small white mouses with the weight of whipworm infection health, chooses and be more than through excrement inspection worm's ovum amount
2000/ gram of small white mouse 180, is divided into 9 groups, every group 20, respectively with the embodiment of the present invention one to
The pressed candy comprising probiotics that embodiment six, comparative example one to comparative example three are prepared carries out gavage.Press
Weight calculating dosage, dosage 0.2mg/kg, once a day；All small white mouses continuously take 3 days.After 3 days, examined with method excrement
Whipworm ovum is checked, and is counted.When being discontinued 5 days, taking small white mouse caecum to examine worm, while collect excrement, the method for being washed with water washes in a pan worm, and with worm
As efficacy assessment index, excrement inspection worm's ovum is cloudy after treatment turns, dissects without worm to be to cure for ovum slip and cure rate.With same
Method test roundworm, pinworm and Diplacanthus nanus.Concrete outcome is as shown in table 1 below.
Table 1 is to causing the repelling and killing efficacy of the parasite of diarrhea
2nd, the effect of diarrhea caused by indigestion is improved
Children (age 1-15 Sui) diarrhea patient 450 caused by recruiting indigestion, 9 groups, every group are divided into by patient
Include 50 children's diarrhae patients；The embodiment of the present invention one to embodiment six, comparative example one to comparative example three is tried out respectively to make
The standby obtained pressed candy for including probiotics.Orally three times a day, each 3g.All patients take 1 day, observe result.Its
In, curative effect situation criterion is to fully recover：Symptom of diarrhea disappears, and stool recovers normal；It is effective：Symptom of diarrhea is obviously improved, abdomen
Number is rushed down to significantly reduce；Effectively：Symptom of diarrhea slightly improves, and diarrhea number is slightly reduced；It is invalid：Symptom of diarrhea changes without any
Kind, concrete outcome is as shown in table 2 below.
Diarrhea patient caused by 2 indigestion of table take 1 day after the effect of compare
3rd, the effect of strengthen immunity
Experimental animal：SPF grades of Female ICR mices, weight are 18~22g, totally 180, are randomly divided into 9 groups, every group 20.
Experimental enviroment condition：Barrier system.
Test method：Assessment of function is carried out by the determination experiment of NK cytoactives.Every group of mouse is real with the present invention respectively
Apply the pressed candy comprising probiotics that example one to embodiment six, comparative example one to comparative example three is prepared and carry out gavage.Press
Weight calculating dosage, dosage 0.2mg/kg, once a day；All small white mouses continuously take 30 days.
Result of the test：It is as shown in table 3 below.
Influence of the table 3 to NK cells in mice activity
4th, antianaphylactic effect
Recruit with the child patient 90 to allergic constitutions such as house dust, mite class, pollen, dandruffs, be randomly divided into 9
Group, carries out allergic skin prick test by every group 10.After rehabilitation every group respectively try out the embodiment of the present invention one to embodiment six,
The pressed candy for including probiotics that comparative example one to comparative example three is prepared.Orally three times a day, each 3g, continues 1
Month, then carry out allergic skin prick test.Obtained result is as shown in table 4 below.
4 antianaphylactic effect of table
Wherein ,-：There is no swelling (red point, diameter are less than 1mm)；
+：Very slight swelling (red point, diameter are less than 3mm)；
++：Swelling diameter is less than 3mm, forms red point；
+++：5mm is arrived in swelling a diameter of 3, while forms red point；
++++：Compared with " +++ " score, swelling diameter bigger, while form red point.
It should be noted that unless otherwise indicated, technical term or scientific terminology used in this application should be this hair
The ordinary meaning that bright one of ordinary skill in the art are understood.Unless specifically stated otherwise, otherwise illustrate in these embodiments
Component and opposite step, numerical expression and the numerical value of step are not limit the scope of the invention.It is illustrated and described herein
In all examples, unless otherwise prescribed, any occurrence should be construed as merely exemplary, not as limitation, because
This, other examples of exemplary embodiment can have different values.
In the description of the present invention, it is to be understood that term " first ", " second " are only used for description purpose, and cannot
It is interpreted as indicating or implies relative importance or imply the quantity of the technical characteristic indicated by indicating.Thus, define " the
One ", one or more this feature can be expressed or be implicitly included to the feature of " second ".In the description of the present invention,
" multiple " are meant that two or more, unless otherwise specifically defined.
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe is described in detail the present invention with reference to foregoing embodiments, it will be understood by those of ordinary skill in the art that：Its according to
Can so modify to the technical solution described in foregoing embodiments, either to which part or all technical characteristic into
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme, it should all cover among protection scope of the present invention.
Claims (10)
- A kind of 1. pressed candy for including probiotics, it is characterised in that raw material components by weight, including：5~10 parts by weight of probiotics, 3~7 weight of 10~15 parts by weight of galactooligosaccharide, 5~8 parts by weight of oligofructose and glucose Measure part.
- 2. the pressed candy according to claim 1 for including probiotics, it is characterised in that：The probiotics includes bifidobacterium lactis, Lactobacillus rhamnosus, lactobacillus paracasei and lactobacillus reuteri.
- 3. the pressed candy according to claim 2 for including probiotics, it is characterised in that：The bifidobacterium lactis, the Lactobacillus rhamnosus, the CFU ratios of the lactobacillus paracasei and the lactobacillus reuteri It is worth for (0.9~1.1):(0.4~0.6):(1.0~1.3):(0.7~0.9).
- 4. the pressed candy according to claim 1 for including probiotics, it is characterised in that raw material components further include：4~6 parts by weight of isomalto-oligosaccharide, 3~5 parts by weight of walnut peptide, 2~4 parts by weight of pea peptide, soybean protein isolate 1.5 1~2 parts by weight of~2.0 parts by weight, 0.5~1.0 parts by weight of fish oil and milk basic protein.
- 5. the pressed candy according to claim 1 for including probiotics, it is characterised in that the preparation method of the walnut peptide Including step：Grease is removed after walnut is crushed, obtains walnut powder；Water is added in the walnut powder, then carries out defibrination, is filtered, collects filtrate, obtains walnut protein slurry；The pH value that the walnut protein is starched is adjusted to 7.0~8.0, trypsase is then added, carries out first time enzymolysis；Then Flavor protease is added, second is carried out and digests；Mixture after described second is digested inactivates, and then filters, and collects filtrate；The filtrate is spray-dried, Obtain walnut peptide.
- 6. the pressed candy according to claim 5 for including probiotics, it is characterised in that：The ratio of the walnut powder and the water is (10~12):100；The addition of the trypsase is starched for 0.3~0.4g/100mL walnut proteins, and the temperature of the first time enzymolysis is 46 ~50 DEG C, the time of the first time enzymolysis is 45~50min；The addition of the flavor protease is starched for 1.6~1.8g/100mL walnut proteins, and the temperature of second of enzymolysis is 50~52 DEG C, the time of second of enzymolysis is 1~1.5h；The temperature of the inactivation is 80~85 DEG C, and the time of inactivation is 25~30min.
- 7. the pressed candy according to claim 1 for including probiotics, it is characterised in that the system of the soybean protein isolate Preparation Method includes step：Defatted soybean meal powder and water are mixed, temperature is heated in alkaline conditions as 70~80 DEG C, extracts preset time, soaked Carry mixture；Addition alkali protease is digested in the extraction mixture, obtains soybean protease hydrolysate；The soybean protease hydrolysate is centrifuged, collects hydrolysate of soybean protein supernatant；By the hydrolysate of soybean protein supernatant tune pH value to 4.2~4.4, the egg in centrifugation hydrolysate of soybean protein supernatant In vain, gained is precipitated and is mixed with water, be freeze-dried after pH value is adjusted to neutrality, obtain soybean protein isolate.
- 8. the pressed candy according to claim 7 for including probiotics, it is characterised in that：The mass ratio of the defatted soybean meal powder and water is (22~25):100, the pH value of the alkaline condition is 8.2~8.4, institute The time for stating extraction is 40~50min；The addition of the alkali protease extracts mixture for 0.35~0.45g/100mL, The time of the enzymolysis is 2~3h；The soybean protease hydrolysate is centrifuged, is that 7000~8000r/min centrifuges 15~18min at 20~30 DEG C；The centrifugation is that 7000~8000r/min centrifuges 23~25min at 20~30 DEG C；Gained precipitates the quality with water Than for (2.5~3.0):1.
- 9. claim 1-8 any one of them includes the preparation method of the pressed candy of probiotics, it is characterised in that including step Suddenly：Other raw material components in addition to probiotics are mixed, are then sprayed into the ethanol water that volume fraction is 50%~75%, Obtain mixed slurry；The mixed slurry is pelletized using wet granulator, then dries, obtains particle；Probiotics is added in the particle, tabletting is carried out using the rotary tablet machines that rush after mixing more, obtains comprising benefit The pressed candy of raw bacterium.
- 10. the preparation method of the pressed candy according to claim 9 comprising probiotics, it is characterised in that：The ratio of the quality of the ethanol water and all raw material components gross masses is 1:(5.4~5.6)；When mixing all raw material components, the step of adding colouring agent, flavor enhancement and binding agent is further included.
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| CN108770977A (en) * | 2018-06-27 | 2018-11-09 | 山东禹王生态食业有限公司 | A kind of compound plant protein pressed candy and preparation method thereof |
| CN109645492A (en) * | 2019-01-28 | 2019-04-19 | 新萃斯生物科技(上海)有限公司 | It is a kind of with the child form composite formula and preparation method thereof for controlling allergy in advance |
| CN111011567A (en) * | 2019-11-28 | 2020-04-17 | 程红 | Tabletting candy with memory improving function and preparation method thereof |
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