CN107898685A - A kind of preparation method and application of Olive leaf P.E - Google Patents
A kind of preparation method and application of Olive leaf P.E Download PDFInfo
- Publication number
- CN107898685A CN107898685A CN201711222150.7A CN201711222150A CN107898685A CN 107898685 A CN107898685 A CN 107898685A CN 201711222150 A CN201711222150 A CN 201711222150A CN 107898685 A CN107898685 A CN 107898685A
- Authority
- CN
- China
- Prior art keywords
- olive leaf
- olive
- 5min
- thimerosal
- leaf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Abstract
The invention discloses a kind of preparation method of Olive leaf P.E, it is related to plant component extraction technical field, includes the following steps：(1) pre-process, the extraction of (2) level-one, the extraction of (3) two level, (4) concentration, purifying.The present invention use two-stage extracting mode, and level-one is extracted using complex enzyme come the cell membrane and cell membrane of rapid damage olive leaf, accelerates the leaching velocity of oleuropein；Complex enzyme further speeds up the leaching velocity of oleuropein by two level extraction again after inactivation is handled, and shortens extraction time, improves the recovery rate of oleuropein.
Description
Technical field：
The present invention relates to plant component extraction technical field, and in particular to a kind of preparation method of Olive leaf P.E and should
With.
Background technology：
Olive, is the rare evergreen woody oleiferous plants in the world characterized by " high yield, high-quality, efficient " and fruit seeds, cultivation
Kind has as many as 500 kinds, about 140 planted extensively kind.Olive growth ability is vigorous, and drought-enduring, light, is mainly distributed on European ground
Zhong Hai coastwise contries and America California area, are passed to China afterwards.For a long time, main be processed into using olive fresh fruit is known as
The olive oil of the title of " vegetable oil queen ".
Modern biotechnology analysis shows, contain substantial amounts of bioactive substance, its oxidation-resistant active ingredient in olive leaf
More prominent compared with olive fruit, especially secoiridoid (oleuropein is main component) is primarily present in European olive leaf
In.
Oleuropein has antibacterial, antiviral activity and extremely strong oxidation resistance, at the same have blood pressure lowering, it is hypoglycemic and
Anticancer etc. acts on.At present, the oleuropein extracting method reported is very much, but the purity of product and yield are all very low, and by
Easily aoxidized in oleuropein, more sensitive to temperature and light, hygroscopicity is very strong, and is not easy to crystallize, so the olive of high-purity
Olive hardship glycosides is not easy to be prepared.
The content of the invention：
The technical problems to be solved by the invention are to provide that a kind of product recovery rate is high, purity is good and it is strong to operate the feature of environmental protection
Olive leaf P.E preparation method.
The technical problems to be solved by the invention are realized using following technical solution：
A kind of preparation method of Olive leaf P.E, includes the following steps：
(1) pre-process：Fresh olive leaf is harvested, injects thimerosal after cleaned rinsing, makes thimerosal complete wetting olive
Leaf, thimerosal temperature are maintained at 35-40 DEG C, and 5min is stood after stirring 5min, continue to stand 5min after stirring 5min, are again stirring for
5min is stood after 5min, drains thimerosal, and with cleaning and rinsing, that is, completes the pretreatment of olive leaf；
(2) level-one is extracted：Water and complex enzyme are injected into pretreated olive leaf, and in 35-40 DEG C of insulated and stirred, 1-
Reflux state insulated and stirred 5-10min is heated to after 3h, up to olive leaf mixture；The complex enzyme is by mass ratio 2:1 it is green
Color trichoderma and trypsase are formed；
(3) two level is extracted：Extracted to level-one in gained olive leaf mixture and add absolute ethyl alcohol, adjust two level Extraction solvent
For the ethanol solution of 70-75%, reflux state insulated and stirred 3-5h is heated to, is filtered, up to extracting solution；
(4) concentrate, purify：Gained extracting solution is concentrated under reduced pressure to recycle ethanol, treats to add equivalent after ethanol recycling
Water recovers to original volume, and filtering, gained filtrate is enriched with once through macroporous absorbent resin HPD-400A, then with 45% ethanol elution,
Eluent is collected, and the medicinal extract of water content 15-20% is made through being concentrated under reduced pressure, medicinal extract is sent into after cooled to room temperature for eluent
In freeze drier, micro mist is made through micronizer in dry obtained solid, up to Olive leaf P.E.
The mass ratio of olive leaf and thimerosal is 10 in the step (1):10-30, the concentration of disinfectant exists in thimerosal
50-100mg/L。
The disinfectant is selected from chlorine dioxide or Polyhaxemethylenguanidine Hydrochloride.
The mass ratio of olive leaf, water and complex enzyme is 10 in the step (2):30-50:0.05-0.1.
The disinfectant is made of the raw material of following weight：Poloxamer188 5-10g, leek tongue 5-10g, gumbo leaf 5-
10g, amaranth root 1-5g, root of couch grass 1-5g, tea ear 1-5g, wild purple perilla 0.5-3g, fragrant camphor tree essential oil 0.01-0.05g, its preparation method
For：Leek tongue, gumbo leaf, amaranth root, root of couch grass, tea ear and wild purple perilla are added in the water of 2 times of gross weights, and are heated to 50-60
DEG C insulated and stirred 30min, recycles power 1.5kW paste mill grinding 30min, filtering, poloxamer is added into gained filtrate
407 and fragrant camphor tree essential oil, it is sent into after being sufficiently mixed in freeze drier, micro mist is made through micronizer in dry obtained solid, i.e.,
Obtain aqueous disinfecting agent.
Application of the Olive leaf P.E in eye cream is prepared.
The beneficial effects of the invention are as follows：
(1) present invention has used disinfectant when olive leaf pre-processes, and the use of disinfectant can not only play olive leaf
Disinfection well, effectively kills the bacterium and germ adhered on olive leaf, moreover it is possible to directly using a small amount of after disinfection
Water can be removed thoroughly；Disinfectant includes two kinds at the same time, and one kind is routine disinfection agent chlorine dioxide or Polyhaxemethylenguanidine Hydrochloride,
Another kind is self-control disinfectant, makes the monarch compatibility pass that the combination of the plant component employed in disinfectant follows Chinese medicine by oneself
System, plays the effect quickly killed to bacterium and germ, so as to improve the safety in utilization of made Olive leaf P.E, and ensures
The vigor of complex enzyme during follow-up multiplex-enzyme extraction, prevents the presence of bacterium and germ from influencing the extraction effect of complex enzyme.
(2) present invention uses two-stage extracting mode, and level-one extraction is using complex enzyme come the cell membrane of rapid damage olive leaf
And cell membrane, accelerate the leaching velocity of oleuropein；Complex enzyme is further speeded up by two level extraction again after inactivation is handled
The leaching velocity of oleuropein, shortens extraction time, improves the recovery rate of oleuropein；
(3) present invention is effectively reducing oleuropein loss amount using macroporous absorbent resin HPD-400A as Purification Resin
On the basis of greatly improve the purity of oleuropein, and macroporous absorbent resin HPD-400A can be recycled after regeneration, keep away
Exempt from disposable seriously to pollute caused by environment；
(4) the made Olive leaf P.E of the present invention can be applied to prepare eye cream, utilize its antioxidant content oleuropein
Eye part skin free radical is eliminated, achievees the purpose that crease-resistant smoothing wrinkle, and can effectively remove melanin, eliminates oedema, promotes eye
Absorption of the skin to nutrients contained by skin care item.
Embodiment：
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention easy to understand, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
(1) pre-process：The fresh olive leaves of 10g are harvested, 20g thimerosals are injected after cleaned rinsing, disinfectant in thimerosal
The concentration of chlorine dioxide makes thimerosal complete wetting olive leaf in 50mg/L, and thimerosal temperature is maintained at 35-40 DEG C, stirring
5min is stood after 5min, continues to stand 5min after stirring 5min, stands 5min after being again stirring for 5min, drain thimerosal, be used in combination
Cleaning and rinsing, that is, complete the pretreatment of olive leaf；
(2) level-one is extracted：30g water and 0.05g complex enzymes are injected into the pretreated olive leaves of 10g, and in 35-40 DEG C
Insulated and stirred, is heated to reflux state insulated and stirred 10min after 2h, up to olive leaf mixture；The complex enzyme is by mass ratio
2:1 Trichoderma viride and trypsase is formed；
(3) two level is extracted：Extracted to level-one in gained olive leaf mixture and add absolute ethyl alcohol, adjust two level Extraction solvent
For 70% ethanol solution, reflux state insulated and stirred 3h is heated to, is filtered, up to extracting solution；
(4) concentrate, purify：Gained extracting solution is concentrated under reduced pressure to recycle ethanol, treats to add equivalent after ethanol recycling
Water recovers to original volume, and filtering, gained filtrate is enriched with once through macroporous absorbent resin HPD-400A, then with 45% ethanol elution,
Eluent is collected, and the medicinal extract of water content 15-20% is made through being concentrated under reduced pressure, medicinal extract is sent into after cooled to room temperature for eluent
In freeze drier, micro mist is made through micronizer in dry obtained solid, up to Olive leaf P.E.
Embodiment 2
(1) pre-process：The fresh olive leaves of 10g are harvested, 20g thimerosals are injected after cleaned rinsing, disinfectant in thimerosal
The concentration of Polyhaxemethylenguanidine Hydrochloride makes thimerosal complete wetting olive leaf, thimerosal temperature is maintained at 35-40 in 50mg/L
DEG C, 5min is stood after stirring 5min, continues to stand 5min after stirring 5min, stands 5min after being again stirring for 5min, drain disinfection
Liquid, and with cleaning and rinsing, that is, complete the pretreatment of olive leaf；
(2) level-one is extracted：30g water and 0.05g complex enzymes are injected into the pretreated olive leaves of 10g, and in 35-40 DEG C
Insulated and stirred, is heated to reflux state insulated and stirred 10min after 2h, up to olive leaf mixture；The complex enzyme is by mass ratio
2:1 Trichoderma viride and trypsase is formed；
(3) two level is extracted：Extracted to level-one in gained olive leaf mixture and add absolute ethyl alcohol, adjust two level Extraction solvent
For 70% ethanol solution, reflux state insulated and stirred 3h is heated to, is filtered, up to extracting solution；
(4) concentrate, purify：Gained extracting solution is concentrated under reduced pressure to recycle ethanol, treats to add equivalent after ethanol recycling
Water recovers to original volume, and filtering, gained filtrate is enriched with once through macroporous absorbent resin HPD-400A, then with 45% ethanol elution,
Eluent is collected, and the medicinal extract of water content 15-20% is made through being concentrated under reduced pressure, medicinal extract is sent into after cooled to room temperature for eluent
In freeze drier, micro mist is made through micronizer in dry obtained solid, up to Olive leaf P.E.
Embodiment 3
(1) pre-process：The fresh olive leaves of 10g are harvested, 20g thimerosals are injected after cleaned rinsing, disinfectant in thimerosal
Concentration in 50mg/L, make thimerosal complete wetting olive leaf, thimerosal temperature is maintained at 35-40 DEG C, is stood after stirring 5min
5min, continues to stand 5min after stirring 5min, stands 5min after being again stirring for 5min, drain thimerosal, and with cleaning and rinsing, i.e.,
Complete the pretreatment of olive leaf；
(2) level-one is extracted：30g water and 0.05g complex enzymes are injected into the pretreated olive leaves of 10g, and in 35-40 DEG C
Insulated and stirred, is heated to reflux state insulated and stirred 10min after 2h, up to olive leaf mixture；The complex enzyme is by mass ratio
2:1 Trichoderma viride and trypsase is formed；
(3) two level is extracted：Extracted to level-one in gained olive leaf mixture and add absolute ethyl alcohol, adjust two level Extraction solvent
For 70% ethanol solution, reflux state insulated and stirred 3h is heated to, is filtered, up to extracting solution；
(4) concentrate, purify：Gained extracting solution is concentrated under reduced pressure to recycle ethanol, treats to add equivalent after ethanol recycling
Water recovers to original volume, and filtering, gained filtrate is enriched with once through macroporous absorbent resin HPD-400A, then with 45% ethanol elution,
Eluent is collected, and the medicinal extract of water content 15-20% is made through being concentrated under reduced pressure, medicinal extract is sent into after cooled to room temperature for eluent
In freeze drier, micro mist is made through micronizer in dry obtained solid, up to Olive leaf P.E.
The preparation of disinfectant：By 10g leek tongue, 5g gumbos leaf, 3g amaranths root, 2g root of couch grass, 2g tea ear and the wild purple perillas of 1g
Add in the water of 2 times of gross weights, and be heated to 50-60 DEG C of insulated and stirred 30min, recycle power 1.5kW paste mill grindings
30min, filtering, 10g poloxamer188s and 0.05g virtue camphor tree essential oils are added into gained filtrate, and it is dry that freezing is sent into after being sufficiently mixed
In dry machine, micro mist is made through micronizer in dry obtained solid, up to disinfectant.
Embodiment 4
(1) pre-process：The fresh olive leaves of 10g are harvested, 20g thimerosals are injected after cleaned rinsing, disinfectant in thimerosal
Concentration in 50mg/L, make thimerosal complete wetting olive leaf, thimerosal temperature is maintained at 35-40 DEG C, is stood after stirring 5min
5min, continues to stand 5min after stirring 5min, stands 5min after being again stirring for 5min, drain thimerosal, and with cleaning and rinsing, i.e.,
Complete the pretreatment of olive leaf；
(2) level-one is extracted：30g water and 0.05g complex enzymes are injected into the pretreated olive leaves of 10g, and in 35-40 DEG C
Insulated and stirred, is heated to reflux state insulated and stirred 10min after 2h, up to olive leaf mixture；The complex enzyme is by mass ratio
2:1 Trichoderma viride and trypsase is formed；
(3) two level is extracted：Extracted to level-one in gained olive leaf mixture and add absolute ethyl alcohol, adjust two level Extraction solvent
For 70% ethanol solution, reflux state insulated and stirred 3h is heated to, is filtered, up to extracting solution；
(4) concentrate, purify：Gained extracting solution is concentrated under reduced pressure to recycle ethanol, treats to add equivalent after ethanol recycling
Water recovers to original volume, and filtering, gained filtrate is enriched with once through macroporous absorbent resin HPD-400A, then with 45% ethanol elution,
Eluent is collected, and the medicinal extract of water content 15-20% is made through being concentrated under reduced pressure, medicinal extract is sent into after cooled to room temperature for eluent
In freeze drier, micro mist is made through micronizer in dry obtained solid, up to Olive leaf P.E.
The preparation of disinfectant：By 10g leek tongue, 5g gumbos leaf, 3g amaranths root, 2g root of couch grass, 2g tea ear and the wild purple perillas of 1g
Add in the water of 2 times of gross weights, and be heated to 50-60 DEG C of insulated and stirred 30min, recycle power 1.5kW paste mill grindings
30min, filtering, 10g poloxamer188s and 0.05g virtue camphor tree essential oils are added into gained filtrate, and it is dry that freezing is sent into after being sufficiently mixed
In dry machine, micro mist is made through micronizer in dry obtained solid, up to aqueous disinfecting agent.
Reference examples 1
(1) pre-process：The fresh olive leaves of 10g are harvested, 20g thimerosals are injected after cleaned rinsing, disinfectant in thimerosal
The concentration of chlorine dioxide makes thimerosal complete wetting olive leaf in 50mg/L, and thimerosal temperature is maintained at 35-40 DEG C, stirring
5min is stood after 5min, continues to stand 5min after stirring 5min, stands 5min after being again stirring for 5min, drain thimerosal, be used in combination
Cleaning and rinsing, that is, complete the pretreatment of olive leaf；
(2) extract：30g water and 0.05g complex enzymes are injected into the pretreated olive leaves of 10g, and in 35-40 DEG C of insulation
Stir, be heated to reflux state insulated and stirred 10min after 5h, the complex enzyme is by mass ratio 2:1 Trichoderma viride and tryptose
Enzyme is formed, filtering, up to extracting solution；
(3) concentrate, purify：Gained extracting solution is concentrated under reduced pressure to recycle ethanol, treats to add equivalent after ethanol recycling
Water recovers to original volume, and filtering, gained filtrate is enriched with once through macroporous absorbent resin HPD-400A, then with 45% ethanol elution,
Eluent is collected, and the medicinal extract of water content 15-20% is made through being concentrated under reduced pressure, medicinal extract is sent into after cooled to room temperature for eluent
In freeze drier, micro mist is made through micronizer in dry obtained solid, up to Olive leaf P.E.
Reference examples 2
(1) pre-process：The fresh olive leaves of 10g are harvested, 20g thimerosals are injected after cleaned rinsing, disinfectant in thimerosal
The concentration of chlorine dioxide makes thimerosal complete wetting olive leaf in 50mg/L, and thimerosal temperature is maintained at 35-40 DEG C, stirring
5min is stood after 5min, continues to stand 5min after stirring 5min, stands 5min after being again stirring for 5min, drain thimerosal, be used in combination
Cleaning and rinsing, that is, complete the pretreatment of olive leaf；
(2) extract：The ethanol solution of injection 70% into 10g pretreated olive leaves, is heated to reflux state insulation
Stir 5h, filtering, up to extracting solution；
(3) concentrate, purify：Gained extracting solution is concentrated under reduced pressure to recycle ethanol, treats to add equivalent after ethanol recycling
Water recovers to original volume, and filtering, gained filtrate is enriched with once through macroporous absorbent resin HPD-400A, then with 45% ethanol elution,
Eluent is collected, and the medicinal extract of water content 15-20% is made through being concentrated under reduced pressure, medicinal extract is sent into after cooled to room temperature for eluent
In freeze drier, micro mist is made through micronizer in dry obtained solid, up to Olive leaf P.E.
Reference examples 3
With reference to 200910311350.9 embodiments 1 of patent CN.
Reference examples 4
With reference to patent CN 200410023233.X embodiments 1.
Embodiment 5
It is utilized respectively embodiment 1-4, reference examples 1-4 the methods measure olive to being extracted with batch equivalent olive leaf
The recovery rate of olive hardship glycosides and the disinfecting power of disinfectant, the results are shown in Table 1.
The extraction result of the olive leaf extracting method of the present invention of table 1
| Group | Recovery rate/% | Bacterium, germ killing rate/% |
| Embodiment 1 | 2.68 | 97.35 |
| Embodiment 2 | 2.75 | 95.64 |
| Embodiment 3 | / | 98.83 |
| Embodiment 4 | / | 98.92 |
| Reference examples 1 | 2.06 | / |
| Reference examples 2 | 1.89 | / |
| Reference examples 3 | 1.75 | / |
| Reference examples 4 | 1.23 | / |
The basic principles, main features and the advantages of the invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (4)
1. a kind of preparation method of Olive leaf P.E, it is characterised in that include the following steps：
(1) pre-process：Fresh olive leaf is harvested, injects thimerosal after cleaned rinsing, makes thimerosal complete wetting olive leaf, disappears
Venom temperature is maintained at 35-40 DEG C, and 5min is stood after stirring 5min, continues to stand 5min after stirring 5min, is again stirring for 5min
After stand 5min, drain thimerosal, and with cleaning and rinsing, that is, complete the pretreatment of olive leaf；
(2) level-one is extracted：Water and complex enzyme are injected into pretreated olive leaf, and after 35-40 DEG C of insulated and stirred, 1-3h
Reflux state insulated and stirred 5-10min is heated to, up to olive leaf mixture；The complex enzyme is by mass ratio 2:1 green wood
Mould and trypsase is formed；
(3) two level is extracted：Extracted to level-one and absolute ethyl alcohol is added in gained olive leaf mixture, adjustment two level Extraction solvent is
The ethanol solution of 70-75%, is heated to reflux state insulated and stirred 3-5h, filtering, up to extracting solution；
(4) concentrate, purify：Gained extracting solution is concentrated under reduced pressure to recycle ethanol, addition equivalent water is extensive after treating ethanol recycling
It is multiple to be enriched with once through macroporous absorbent resin HPD-400A to original volume, filtering, gained filtrate, then with 45% ethanol elution, collect
The medicinal extract of water content 15-20% is made through being concentrated under reduced pressure, is sent into medicinal extract after cooled to room temperature and freezes for eluent, eluent
In drying machine, micro mist is made through micronizer in dry obtained solid, up to Olive leaf P.E.
2. the preparation method of Olive leaf P.E according to claim 1, it is characterised in that：Olive in the step (1)
The mass ratio of leaf and thimerosal is 10:10-30, the concentration of disinfectant is in 50-100mg/L in thimerosal.
3. the preparation method of Olive leaf P.E according to claim 2, it is characterised in that：The disinfectant is selected from dioxy
Change chlorine or Polyhaxemethylenguanidine Hydrochloride.
4. the preparation method of Olive leaf P.E according to claim 1, it is characterised in that：Olive in the step (2)
The mass ratio of leaf, water and complex enzyme is 10:30-50:0.05-0.1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711222150.7A CN107898685A (en) | 2017-11-29 | 2017-11-29 | A kind of preparation method and application of Olive leaf P.E |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711222150.7A CN107898685A (en) | 2017-11-29 | 2017-11-29 | A kind of preparation method and application of Olive leaf P.E |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107898685A true CN107898685A (en) | 2018-04-13 |
Family
ID=61849119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711222150.7A Pending CN107898685A (en) | 2017-11-29 | 2017-11-29 | A kind of preparation method and application of Olive leaf P.E |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107898685A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102451235A (en) * | 2010-10-27 | 2012-05-16 | 深圳劲创生物技术有限公司 | Preparation method of olive leaf extract |
| CN105055527A (en) * | 2015-09-25 | 2015-11-18 | 白心亮 | Preparation method of olive leaf extract |
| CN106900647A (en) * | 2017-02-20 | 2017-06-30 | 和县凤台山煜新畜牧有限公司 | A kind of feeding and management method of summer sow due to farrow |
-
2017
- 2017-11-29 CN CN201711222150.7A patent/CN107898685A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102451235A (en) * | 2010-10-27 | 2012-05-16 | 深圳劲创生物技术有限公司 | Preparation method of olive leaf extract |
| CN105055527A (en) * | 2015-09-25 | 2015-11-18 | 白心亮 | Preparation method of olive leaf extract |
| CN106900647A (en) * | 2017-02-20 | 2017-06-30 | 和县凤台山煜新畜牧有限公司 | A kind of feeding and management method of summer sow due to farrow |
Non-Patent Citations (2)
| Title |
|---|
| 卢海博等: "植物性杀菌剂研究进展", 《河北农业科学》 * |
| 粮食大辞典编辑委员会编: "《粮食大辞典》", 31 December 2009, 中国物资出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111096940B (en) | Mite-removing itching-relieving hair washing preparation containing sweet wormwood | |
| CN106667837A (en) | Camellia oleifera component based body lotion and preparation method thereof | |
| CN102793028B (en) | Production process of composite functional plant tea drink | |
| KR101054378B1 (en) | Herbal soap composition and its manufacturing method | |
| CN105434256A (en) | Natural emulsion, and preparation method and applications thereof | |
| CN104472678B (en) | Melon preservative and preservation method thereof | |
| CN111110574B (en) | Wet tissue with three-in-one functions of cleaning, sterilizing and moisturizing and preparation method thereof | |
| CN107347984B (en) | Passion fruit preservative and preparation method and application thereof | |
| CN108403522A (en) | A kind of four seasons series toner based on Plant flawer extracts | |
| CN101927019A (en) | Sterilizing and deodorizing agent and preparation method thereof | |
| CN107535651A (en) | Russia's color black tea and preparation method thereof | |
| CN108066228B (en) | Moisturizing antibacterial shower gel and preparation method thereof | |
| CN107898685A (en) | A kind of preparation method and application of Olive leaf P.E | |
| CN109602623A (en) | A kind of bamboo liquid skin care item and preparation method thereof | |
| CN110200028A (en) | A kind of cane planting insecticide and preparation method thereof | |
| CN108587331A (en) | A kind of special white-out agent of citrus fruit | |
| CN107184467A (en) | A kind of hair care exterior-applied article being made of Lignum Xanthoceratis extract and preparation method thereof | |
| CN106491451A (en) | The extracting method of root of Broussonetiapapyrifera(I)vent effective ingredient and its prepare the purposes of cosmetics | |
| CN107184507A (en) | A kind of snowball herbal skin care product | |
| CN106619335A (en) | Preparation method and application of green plum extract | |
| CN111937906A (en) | Plant growth regulating mixture and preparation method and application thereof | |
| CN113662994A (en) | Extraction method of peach blossom cell water and application of peach blossom cell water | |
| CN113559025A (en) | Extraction method of cherry blossom cell water and application of obtained cherry blossom cell water | |
| CN106667878A (en) | Moisturizing and whitening mask liquid containing herba dendrobii polysaccharides | |
| CN110151662A (en) | A kind of plant extract face-nourishing liquor and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180413 |
|
| RJ01 | Rejection of invention patent application after publication |