CN107873947A - 一种复合发酵生物秸秆微胶囊饲料的生产方法 - Google Patents

一种复合发酵生物秸秆微胶囊饲料的生产方法 Download PDF

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CN107873947A
CN107873947A CN201711186864.7A CN201711186864A CN107873947A CN 107873947 A CN107873947 A CN 107873947A CN 201711186864 A CN201711186864 A CN 201711186864A CN 107873947 A CN107873947 A CN 107873947A
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高绍彬
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Abstract

一种复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,其包括,复合菌的活化与扩大培养,秸秆粉碎预处理,固态发酵秸秆,制备饲料内芯原颗粒,真空喷涂植酸酶和复合维生素,包膜。本发明的微胶囊饲料,以发酵秸秆为蛋白质源,采用真空喷涂技术和包膜技术协同作用,改善热敏性成分损失的问题;该饲料营养全面,可以大幅度减少反刍动物的甲烷排放量,可以降低甲烷能的损失,提高日粮的能量消化率及饲料净能,减少了畜牧养殖对环境的污染。

Description

一种复合发酵生物秸秆微胶囊饲料的生产方法
技术领域
本发明属于饲料领域,具体涉及一种复合发酵生物秸秆微胶囊饲料的生产方法。
背景技术
饲料微胶囊化可以保护敏感的营养组分(如益生菌、酶制剂、维生素等);有效地减少活性物质受外界环境因素(如光、氧、水、热等)的影响,改变芯材的物理性质(如颜色、形状、密度、分散性等)和化学性质等;控制芯材的释放速度。经微胶囊化后的微粒饲料可人为控制其粒径和密度,有利于仔稚幼体的摄食,同时可减少营养成分在水体中的溶失。微胶囊饲料相对于微粘饲料而言在水中的稳定性更好,而且具有延缓活性物质的释放速度、增加营养物质的稳定性等特点。
发明内容
本发明的目的在于提供一种营养全面的复合发酵生物秸秆微胶囊饲料的生产方法。
为了实现上述的目的,本发明采用了如下技术方案:
一种复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,其包括,复合菌的活化与扩大培养,秸秆粉碎预处理,固态发酵秸秆,制备饲料内芯原颗粒,真空喷涂植酸酶和复合维生素,包膜。
所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,其具体步骤为:
(1)在无菌条件下,将白腐真菌、康氏木霉和酵母菌种接种到马铃薯葡萄糖琼脂培养基上,放置在恒温箱内,在30-35℃恒温培养,活化72h,得到优质菌种;在无菌条件下,用10mL无菌水冲洗马铃薯葡萄糖琼脂培养基上已活化的菌种,接种至扩大培养培养基中,置于摇床中,在30-35℃条件下,160-180r/min震荡培养72h,得到培养完成的扩大培养液;
(2)先使用高喷侧草粉碎机分别将玉米秸秆和水稻秸秆剪切至2-4cm的小段,再将剪切后的玉米秸秆和水稻秸秆以1:2-3的质量比混合,再利用多功能饲料粉碎机将秸秆粉碎,过40-60目筛,得到秸秆粉;
(3)将培养完成的扩大培养液加入到4-5倍重量份的矿物元素营养液中,充分混合后,再添加4-5倍重量份的生理盐水;搅拌后均匀喷洒到秸秆粉中,不停搅拌,继续添加生理盐水至抓取秸秆粉有湿润感,捏紧以后有水渗出但不滴下,松手后秸秆会自动散开而不成团,此时料水比约为1:1.5-1.8;将混匀后的秸秆粉加入到封口袋中,排尽空气后封口,置于阴凉避光处自然条件下进行发酵30-35d;
(4)将固体发酵后的秸秆粉、鱼油、小麦粉和海藻酸钠加入到槽型混合机,搅拌均匀后加入纯净水,调节体系的含水量为7-8%,在槽型混合机以转速22-25r/min,倒料角度为100-105°的工艺条件混合20-30min,得到湿料;将湿料注入环辗刀的旋转式颗粒机中,经旋转挤压,通过40目的网孔形成湿粒;将粒投入到旋转离心盘内,启动鼓风机鼓60-70℃的热风,再将以10-12r/min抛圆30-40min,得到内芯圆颗粒。
(5)将植酸酶、复合维生素加入到蒸馏水中混合搅拌均匀,得到植酸酶溶液;将乙基纤维素加入到4-5倍的无水乙醇中,得到乙基纤维素溶液;
(6)在常压下将内芯圆颗粒加入到真空喷涂设备中,开始抽除真空使得空气,当压力降为0.02-0.04MPa时,开始喷涂植酸酶溶液溶液,喷涂时混合机构不断混合内芯圆颗粒,以便喷涂均匀;喷涂混合停止后,不断升高压力直到常压,开始混合喷涂乙基纤维素溶液,喷涂结束后,启动鼓风机鼓40-50℃的热风,继续混合15-20min,得到复合发酵生物秸秆微胶囊饲料。
所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(1)中的白腐真菌、康氏木霉和酵母菌种的质量比为1:1.2-1.3:1.8-2,其总接种量为0.2-0.4%。
所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(1)中马铃薯葡萄糖琼脂培养基为:将去皮处理的马铃薯200g切成块状,在1000mL蒸馏水中煮沸30-40min,滤去块状马铃薯,得到马铃薯汁;向马铃薯汁中加入蒸馏水定容至1000mL,再加入15g葡萄糖和20g琼脂,混合搅拌均匀后,110-120℃灭菌25-30min,取出斜面放置,冷却。
所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(1)中扩大培养培养基为:将去皮处理的马铃薯200g切成块状,在1000mL蒸馏水中煮沸30-40min,滤去块状马铃薯,得到马铃薯汁;向马铃薯汁中加入蒸馏水定容至1000mL,再加入15g葡萄糖,混合搅拌均匀后,110-120℃灭菌25-30min,取出斜面放置,冷却。
所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(3)中矿物元素营养液:硫酸铵1.4g,磷酸二氢钾3.0g,硫酸镁0.6g,氯化钙0.3g,氯化钠0.5g,硫酸铁0.05g,硫酸锰0.02g,氯化锌0.02g,氯化钴0.005g,加入到1000mL的蒸馏水中,混合搅拌至充分溶解。
所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(4)中固体发酵后的秸秆粉、鱼油、小麦粉和海藻酸钠的质量比为1:0.3-0.4:0.5-0.6:0.03-0.04。
所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(5)中植酸酶溶液中植酸酶的浓度为1000U/mL,复合维生素的浓度为0.02-0.03%。
所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(6)中内芯圆颗粒、植酸酶溶液和乙基纤维素溶液的质量比为1:0.12-0.15:0.15-0.18。
通过上述的技术方案,本发明的有益效果是:
本发明采用白腐真菌、康氏木霉和酵母菌种为复合菌剂固体发酵玉米秸秆和水稻秸秆,以发酵秸秆为主要原料制备的饲料,能明显提商饲料营养成分,改善适口性,并有效改善畜禽肠道微生态区系,促进畜禽生长,提髙养殖收入;同时,可以大幅度减少反刍动物的甲烷排放量,可以降低甲烷能的损失,提高日粮的能量消化率及饲料净能,减少了畜牧养殖对环境的污染。本发明的微胶囊饲料,以发酵秸秆为蛋白质源,鱼油为脂肪源,小麦粉为糖源,辅以植酸酶、复合维生素,营养全面,有利于牲畜对饲料的吸收,提高牲畜的品质;采用真空喷涂技术和包膜技术协同作用,可以保护敏感的营养组分;有效地减少活性物质受外界环境因素的影响,控制芯材的释放速度。
具体实施方式
本实施例的复合发酵生物秸秆微胶囊饲料的生产方法,其具体步骤为:
(1)在无菌条件下,将白腐真菌、康氏木霉和酵母菌种接种到马铃薯葡萄糖琼脂培养基上,放置在恒温箱内,在30℃恒温培养,活化72h,得到优质菌种;在无菌条件下,用10mL无菌水冲洗马铃薯葡萄糖琼脂培养基上已活化的菌种,接种至扩大培养培养基中,置于摇床中,在30℃条件下,160r/min震荡培养72h,得到培养完成的扩大培养液;
(2)先使用高喷侧草粉碎机分别将玉米秸秆和水稻秸秆剪切至2cm的小段,再将剪切后的玉米秸秆和水稻秸秆以1:3的质量比混合,再利用多功能饲料粉碎机将秸秆粉碎,过60目筛,得到秸秆粉;
(3)将培养完成的扩大培养液加入到5倍重量份的矿物元素营养液中,充分混合后,再添加5倍重量份的生理盐水;搅拌后均匀喷洒到秸秆粉中,不停搅拌,继续添加生理盐水至抓取秸秆粉有湿润感,捏紧以后有水渗出但不滴下,松手后秸秆会自动散开而不成团,此时料水比约为1:1.8;将混匀后的秸秆粉加入到封口袋中,排尽空气后封口,置于阴凉避光处自然条件下进行发酵35d;
(4)将固体发酵后的秸秆粉、鱼油、小麦粉和海藻酸钠加入到槽型混合机,搅拌均匀后加入纯净水,调节体系的含水量为8%,在槽型混合机以转速25r/min,倒料角度为105°的工艺条件混合30min,得到湿料;将湿料注入环辗刀的旋转式颗粒机中,经旋转挤压,通过40目的网孔形成湿粒;将粒投入到旋转离心盘内,启动鼓风机鼓60℃的热风,再将以12r/min抛圆30min,得到内芯圆颗粒。
(5)将植酸酶、复合维生素加入到蒸馏水中混合搅拌均匀,得到植酸酶溶液;将乙基纤维素加入到5倍的无水乙醇中,得到乙基纤维素溶液;
(6)在常压下将内芯圆颗粒加入到真空喷涂设备中,开始抽除真空使得空气,当压力降为0.03MPa时,开始喷涂植酸酶溶液溶液,喷涂时混合机构不断混合内芯圆颗粒,以便喷涂均匀;喷涂混合停止后,不断升高压力直到常压,开始混合喷涂乙基纤维素溶液,喷涂结束后,启动鼓风机鼓50℃的热风,继续混合20min,得到复合发酵生物秸秆微胶囊饲料。
本实施例的步骤(1)中的白腐真菌、康氏木霉和酵母菌种的质量比为1:1.3:2,其总接种量为0.2%。
本实施例的步骤(1)中马铃薯葡萄糖琼脂培养基为:将去皮处理的马铃薯200g切成块状,在1000mL蒸馏水中煮沸30min,滤去块状马铃薯,得到马铃薯汁;向马铃薯汁中加入蒸馏水定容至1000mL,再加入15g葡萄糖和20g琼脂,混合搅拌均匀后,120℃灭菌25-30min,取出斜面放置,冷却。
本实施例的步骤(1)中扩大培养培养基为:将去皮处理的马铃薯200g切成块状,在1000mL蒸馏水中煮沸30min,滤去块状马铃薯,得到马铃薯汁;向马铃薯汁中加入蒸馏水定容至1000mL,再加入15g葡萄糖,混合搅拌均匀后,120℃灭菌25-30min,取出斜面放置,冷却。
本实施例的步骤(3)中矿物元素营养液:硫酸铵1.4g,磷酸二氢钾3.0g,硫酸镁0.6g,氯化钙0.3g,氯化钠0.5g,硫酸铁0.05g,硫酸锰0.02g,氯化锌0.02g,氯化钴0.005g,加入到1000mL的蒸馏水中,混合搅拌至充分溶解。
本实施例的步骤(4)中固体发酵后的秸秆粉、鱼油、小麦粉和海藻酸钠的质量比为1:0.4:0.6:0.04。
本实施例的步骤(5)中植酸酶溶液中植酸酶的浓度为1000U/mL,复合维生素的浓度为0.03%。
本实施例的步骤(6)中内芯圆颗粒、植酸酶溶液和乙基纤维素溶液的质量比为1:0.15:0.18。

Claims (9)

1.一种复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,其包括,复合菌的活化与扩大培养,秸秆粉碎预处理,固态发酵秸秆,制备饲料内芯原颗粒,真空喷涂植酸酶和复合维生素,包膜。
2.根据权利要求书1所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,其具体步骤为:
(1)在无菌条件下,将白腐真菌、康氏木霉和酵母菌种接种到马铃薯葡萄糖琼脂培养基上,放置在恒温箱内,在30-35℃恒温培养,活化72h,得到优质菌种;在无菌条件下,用10mL无菌水冲洗马铃薯葡萄糖琼脂培养基上已活化的菌种,接种至扩大培养培养基中,置于摇床中,在30-35℃条件下,160-180r/min震荡培养72h,得到培养完成的扩大培养液;
(2)先使用高喷侧草粉碎机分别将玉米秸秆和水稻秸秆剪切至2-4cm的小段,再将剪切后的玉米秸秆和水稻秸秆以1:2-3的质量比混合,再利用多功能饲料粉碎机将秸秆粉碎,过40-60目筛,得到秸秆粉;
(3)将培养完成的扩大培养液加入到4-5倍重量份的矿物元素营养液中,充分混合后,再添加4-5倍重量份的生理盐水;搅拌后均匀喷洒到秸秆粉中,不停搅拌,继续添加生理盐水至抓取秸秆粉有湿润感,捏紧以后有水渗出但不滴下,松手后秸秆会自动散开而不成团,此时料水比约为1:1.5-1.8;将混匀后的秸秆粉加入到封口袋中,排尽空气后封口,置于阴凉避光处自然条件下进行发酵30-35d;
(4)将固体发酵后的秸秆粉、鱼油、小麦粉和海藻酸钠加入到槽型混合机,搅拌均匀后加入纯净水,调节体系的含水量为7-8%,在槽型混合机以转速22-25r/min,倒料角度为100-105°的工艺条件混合20-30min,得到湿料;将湿料注入环辗刀的旋转式颗粒机中,经旋转挤压,通过40目的网孔形成湿粒;将粒投入到旋转离心盘内,启动鼓风机鼓60-70℃的热风,再将以10-12r/min抛圆30-40min,得到内芯圆颗粒;
(5)将植酸酶、复合维生素加入到蒸馏水中混合搅拌均匀,得到植酸酶溶液;将乙基纤维素加入到4-5倍的无水乙醇中,得到乙基纤维素溶液;
(6)在常压下将内芯圆颗粒加入到真空喷涂设备中,开始抽除真空使得空气,当压力降为0.02-0.04MPa时,开始喷涂植酸酶溶液溶液,喷涂时混合机构不断混合内芯圆颗粒,以便喷涂均匀;喷涂混合停止后,不断升高压力直到常压,开始混合喷涂乙基纤维素溶液,喷涂结束后,启动鼓风机鼓40-50℃的热风,继续混合15-20min,得到复合发酵生物秸秆微胶囊饲料。
3.根据权利要求书2所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(1)中的白腐真菌、康氏木霉和酵母菌种的质量比为1:1.2-1.3:1.8-2,其总接种量为0.2-0.4%。
4.根据权利要求书2所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(1)中马铃薯葡萄糖琼脂培养基为:将去皮处理的马铃薯200g切成块状,在1000mL蒸馏水中煮沸30-40min,滤去块状马铃薯,得到马铃薯汁;向马铃薯汁中加入蒸馏水定容至1000mL,再加入15g葡萄糖和20g琼脂,混合搅拌均匀后,110-120℃灭菌25-30min,取出斜面放置,冷却。
5.根据权利要求书2所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(1)中扩大培养培养基为:将去皮处理的马铃薯200g切成块状,在1000mL蒸馏水中煮沸30-40min,滤去块状马铃薯,得到马铃薯汁;向马铃薯汁中加入蒸馏水定容至1000mL,再加入15g葡萄糖,混合搅拌均匀后,110-120℃灭菌25-30min,取出斜面放置,冷却。
6.根据权利要求书2所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(3)中矿物元素营养液:硫酸铵1.4g,磷酸二氢钾3.0g,硫酸镁0.6g,氯化钙0.3g,氯化钠0.5g,硫酸铁0.05g,硫酸锰0.02g,氯化锌0.02g,氯化钴0.005g,加入到1000mL的蒸馏水中,混合搅拌至充分溶解。
7.根据权利要求书2所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(4)中固体发酵后的秸秆粉、鱼油、小麦粉和海藻酸钠的质量比为1:0.3-0.4:0.5-0.6:0.03-0.04。
8.根据权利要求书2所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(5)中植酸酶溶液中植酸酶的浓度为1000U/mL,复合维生素的浓度为0.02-0.03%。
9.根据权利要求书2所述的复合发酵生物秸秆微胶囊饲料的生产方法,其特征在于,步骤(6)中内芯圆颗粒、植酸酶溶液和乙基纤维素溶液的质量比为1:0.12-0.15:0.15-0.18。
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