CN107865423A - A kind of SOD oral liquids of anti-enteron aisle oxidation - Google Patents
A kind of SOD oral liquids of anti-enteron aisle oxidation Download PDFInfo
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- CN107865423A CN107865423A CN201711103093.0A CN201711103093A CN107865423A CN 107865423 A CN107865423 A CN 107865423A CN 201711103093 A CN201711103093 A CN 201711103093A CN 107865423 A CN107865423 A CN 107865423A
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- sod
- jujube
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- 206010012735 Diarrhoea Diseases 0.000 description 2
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
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- 108010070915 orgotein Proteins 0.000 description 1
- 229960004534 orgotein Drugs 0.000 description 1
- 150000002926 oxygen Chemical class 0.000 description 1
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- TUGDLVFMIQZYPA-UHFFFAOYSA-N tetracopper;tetrazinc Chemical compound [Cu+2].[Cu+2].[Cu+2].[Cu+2].[Zn+2].[Zn+2].[Zn+2].[Zn+2] TUGDLVFMIQZYPA-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to food technology manufacture field, disclosing one kind can resist enteron aisle to aoxidize SOD oral liquids, the oral liquid is mainly using marine low temperature SOD as primary raw material, addition safflower extract, Fructus lycii P.E, Rhizoma Chuanxiong extract, jujube concentrate stir vacuum packaging, produce, its specific proportioning is：The anti-enteron aisle oxidation SOD oral liquids of the present invention, directly take in postprandial half an hour.The method have the characteristics that：Marine low temperature SOD：2 ~ 3 parts, crude extract from carthamus tinctorius L. with water：1 ~ 2 part, fruit of Chinese wolfberry water extract：1 ~ 2 part, Ligusticum wallichii water extract：1 ~ 2 part, 3 ~ 4 parts of jujube concentrate.The present invention by marine low temperature SOD and traditional Chinese medicine extract perfect adaptation, advantageously reduces intestinal tract injury caused by oxidative stress, for maintaining animal intestinal tract and holistic health to have great importance by the compatibility of science safety.
Description
Technical field
The present invention relates to food technology manufacture field, and in particular to one kind can resist enteron aisle to aoxidize SOD oral liquids.
Background technology
Enteron aisle is a part for digestion, not only with digestion and absorption function, and is that human body is maximum and is immunized
Organ.When pathogenic microorganism invades enteron aisle, enteron aisle inherent immunity System Take local defense effect.Enteron aisle inherent immunity system
System mainly includes intestinal mucosa epithelial barrier, inherent immunity cell, inherent immunity molecule etc..Wherein function of intestinal mucosa barrier in patient is enteron aisle
A key character, when external environment, stress, the factor such as infection stimulate enteron aisle when can break normal bacterium in intestinal mucosal barrier
Balance between group and host, enteron aisle homeostasis is caused to lack of proper care.Therefore, enteron aisle is extremely important to health.
The content of the invention
The invention aims to protect human body intestinal canal healthy, there is provided a kind of SOD oral liquids that enteron aisle can be resisted to aoxidize.
For achieving the above object, the present invention uses following technical scheme：
A kind of SOD oral liquids of anti-enteron aisle oxidation, using marine low temperature SOD as primary raw material, addition crude extract from carthamus tinctorius L. with water, the fruit of Chinese wolfberry
Water extract, Ligusticum wallichii water extract, jujube concentrate are mixed and are prepared, and in parts by weight, proportioning is as follows：
Marine low temperature SOD：2 ~ 3 parts,
Crude extract from carthamus tinctorius L. with water：1 ~ 2 part,
Fruit of Chinese wolfberry water extract：1 ~ 2 part,
Ligusticum wallichii water extract：1 ~ 2 part,
3 ~ 4 parts of jujube concentrate.
Further, marine low temperature SOD preparation method comprises the following steps：
（1）The preparation of crude enzyme liquid：Sterile working, the sea activated is sticked into Pseudoalteromonas and is inoculated into liquid fermentation medium
In, in 20 DEG C, 160r/min fermentation 48h, zymocyte liquid obtains wet thallus in 4 DEG C, 6000r/min centrifugation 30min, 4 DEG C,
6000r/min centrifuges 30min, and wet thallus dissolves with phosphate buffer, crushed under 480W ultrasonic wave ice baths 30min (broken 5s,
It is spaced 5s), after crushing, 4 DEG C, 12 000r/min centrifugation 15min, supernatant is crude enzyme liquid, aforesaid liquid fermentative medium formula
Percentage is：The .0 of glucose 2, yeast extract 0 .5, Chen Haishui preparation, pH are natural；
（2）Ammonium sulfate precipitation：6 parts of 25ml crude enzyme liquids are prepared, the cold ammonium sulfate of solid is added thereto respectively, makes its saturated solution dense
Degree difference 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 4 DEG C of 16h that saltout, the dissolving of pH7 .8 phosphate buffers
Protein precipitation, determine low-temperature superoxide dismutase activity highest ammonium sulfate precipitation condition；
（3）It is concentrated by ultrafiltration：It will saltout and complete sample and be transferred to molecular cut off 10KDa super filter tubes by pretreatment, 4 DEG C,
6000r/min, ultrafiltration 30min, remove SO4 2-With part small molecular protein, liquid-transfering gun carefully takes out concentrate, and 4 DEG C, preservation is standby
With being partially soluble in phosphate buffer, determine low-temperature superoxide dismutase enzyme activity, calculate protein recovery；
（4）Sephadex G-100 column chromatographies：Sephadex G-100 gel columns are balanced with pH7 .8 phosphate buffers, treat baseline
After balance, enzyme liquid adds the equilibrated Sephadex G-100 gel columns (Ф 1 of pH7 .8 phosphate buffers after taking 2ml ultrafiltration
.6cm × 60cm), regulation sets automatic collector, and often pipe collects 2ml or so, according to the eluting peak on display, it is determined that containing low
Warm superoxide dismutase is respectively managed, and is collected and is determined its enzyme activity, and merging is respectively managed containing enzyme activity, and 4 DEG C, preservation is standby；
（5）Spray drying：The parameter of spray drying is arranged to 110 DEG C of inlet temperature, outlet temperature is 60 DEG C, pressure position 2kgf/
cm2, flow rate of liquid 5mL/min, trehalose, sucrose, the dosage of sodium alginate are 4% in the protective agent in spray drying, can
The dosage of soluble starch is 7%, protectant total dosage control obtains low-temperature superoxide dismutase within 10%.
Further, Chinese medicine（Safflower, the fruit of Chinese wolfberry and Ligusticum wallichii）The preparation method of extract is as follows：
Chinese medicine（Safflower, the fruit of Chinese wolfberry and Ligusticum wallichii）20g is weighed, adds 400mL deionized waters immersion 24h, moderate heat boils 3h, filtered,
Filter residue adds 200mL deionized waters and extracted 1 time with same method, merges 2 filtrates and is concentrated into 100mL, that is, obtains quality
Concentration is 0.2g/mL (20%) Chinese herbal medicine Aqueous extracts, puts 4 DEG C and saves backup.
Further, the preparation method of jujube concentrate is as follows：
Jujube dry product is taken, 85 DEG C are heated in steam copper and is kept for 15 minutes, the jujube after heating is ground into mud；To after crushing
Jujube paste add pectase, after neutral proteinase, cellulase are sufficiently mixed, mixture temperature is maintained at 25 DEG C of fermentations 1.5
Hour；The jujube material that ferment treatment is crossed is placed in pure water and soaked, pure coolant-temperature gage is maintained at 27 DEG C, soak time 2.5
Hour, do not stop to stir mixture in immersion process；The mixture of the jujube of immersion is filtered in filter, obtains Chinese date juice；
And concentrate Chinese date juice in vacuum decker 45 minutes, Chinese date juice is maintained at 27 DEG C；Finally inspissated juice is added in autoclave
Heat keeps being sterilized for 5 ~ 10 minutes to 90 DEG C；Jujube inspissated juice after sterilizing is cooled into 4 DEG C to save backup.
Marine low temperature SOD is mixed by weight ratio：2 ~ 3 parts, crude extract from carthamus tinctorius L. with water：1 ~ 2 part, fruit of Chinese wolfberry water extract：1
~ 2 parts, Ligusticum wallichii water extract：1 ~ 2 part, 3 ~ 4 parts of jujube concentrate, it is well mixed that marine low temperature SOD oral liquids are made, in postprandial
Half an hour directly takes.
The primary raw material of sea dietary of the present invention is：
1. superoxide dismutase (the .15 .1 .1 of superoxide dismutase, EC 1, alias orgotein, SOD,
Abbreviation SOD) it is a kind of enzyme that can be catalyzed superoxides and oxygen and hydrogen peroxide are converted into by disproportionated reaction, effectively defend
Injury of the free radical to organism, it is free radical scavenger optimal in organism, there is anti-inflammatory, antiviral, radioresistance, resist
Aging etc. acts on, and is widely used in the fields such as medicine, cosmetics, food.
Marine microorganism has the characteristic such as low temperature, pressure-resistant, thermophilic salt, its SOD secreted to adapt to extreme marine environment
Also many valuable enzymatic properties are evolved, such as there is higher resistance to temperature, also shown in terms of physics and chemistry is resistant to compared with
High stability etc..Marine low temperature enzyme has high vigor and high catalytic efficiency under low temperature, is substantially shorter processing time and saves
Expensive heating/cooling system, thus have sizable progress in terms of energy-conservation；It can make cold-adapted enzyme by gentle heat treatment
Vigor lose, and low temperature or thermophilic processing do not interfere with the characteristics such as the quality of product.In view of low temperature SOD is in nature and physiology temperature
Application advantage under degree, marine low temperature SOD are with a wide range of applications and opened in health care, food, cosmetics etc.
Send out potentiality.
2. the fruit of Chinese wolfberry
Fruit of Chinese wolfberry medicinal and edible plant, sweet, mild-natured, Return liver, kidney, lung channel, nourishing the liver, nourshing kidney, moistening lung.Contain abundant carrot
The anti-oxidation active substances such as element, vitamin E, selenium and flavonoids, a variety of antioxidation activity things of the antioxidation contained by it
The coefficient result of matter.LBP-X is also antioxidant content important in matrimony vine, can significantly reduce experimental animal Splenic vessel
LPO contents, and to CCl4Caused lipid anti-oxidative damage has obvious protective function.
3. Ligusticum wallichii (Ligusticum chuanxiong Hort.)
Ligusticum wallichii acrid flavour, it is warm-natured.Return liver, courage, pericardium channel, there is the effect of promoting blood circulation and removing blood stasis, wind-expelling pain-stopping.Ligusticum wallichii alcohol extract ligustrazine
Serum transaminase can be reduced, maintains and improve SOD activity in hepatic tissue；Scavenging activated oxygen, its toxicity is reduced, had good
Anti peroxidation of lipid damaging action, there is obvious antioxidation to enteron aisle, reduce intestinal tract injury.
4. safflower (Carthamus tinctorius L.)
Safflower is traditional medicine, has the effect of invigorate blood circulation, blood stasis removing analgesic, for treating circulation disorder.Its effectively into
Divide mainly carthamin yellow and safflower red pigment.Wherein, carthamin yellow has scavenging hydroxyl, suppresses lipid peroxy
Change, protect nerve from the effect such as damage.
5. jujube
Jujube is a kind of nutrient excellent product, is described as " kings of all kinds of fruits ".It is warm-natured, sweet, described in Chinese herbal medicine books " this warp "
Arrive, sweet warm-natured, the returns spleen stomach of jujube, there is tonifying middle-Jiao and Qi, nourishing blood and tranquilization, the function of relaxing the property of medicine.
Compared with prior art, beneficial effects of the present invention are：The present invention is using marine low temperature SOD as primary raw material, with red
Flower water extract, fruit of Chinese wolfberry water extract, Ligusticum wallichii water extract, jujube concentrate are auxiliary material, will by the compatibility of science safety
Marine low temperature SOD and traditional Chinese medicine extract perfect adaptation, advantageously reduce enteron aisle microbiological contamination, ulcer etc. caused by easy inflammation disease,
Prominent enhancing oxidation resistant effect of enteron aisle.Oral liquid made of the present invention is free of any chemical composition, green safe, easy absorption,
With good anti-oxidant, removing free radical isoreactivity.
Embodiment
With reference to specific embodiment, the invention will be further described.
Embodiment 1
The present embodiment selection marine low temperature SOD, crude extract from carthamus tinctorius L. with water, fruit of Chinese wolfberry water extract, Ligusticum wallichii water extract, jujube concentration
Liquid is prepared by following weight：Marine low temperature SOD：2.5 parts, crude extract from carthamus tinctorius L. with water：1.5 parts, the extraction of fruit of Chinese wolfberry water
Thing：1 part, Ligusticum wallichii water extract：2 parts, 3 parts of jujube concentrate；Above-mentioned raw materials are mixed, stir vacuum packaging, in postprandial
Half an hour directly takes.
Wherein, marine low temperature SOD preparation method is as follows：
(1) preparation of crude enzyme liquid：Sterile working, the sea activated is sticked into Pseudoalteromonas and is inoculated into liquid fermentation medium
In, in 20 DEG C, 160r/min fermentation 48h, zymocyte liquid obtains wet thallus in 4 DEG C, 6000r/min centrifugation 30min, 4 DEG C,
6000r/min centrifuges 30min, and wet thallus dissolves with phosphate buffer, crushed under 480W ultrasonic wave ice baths 30min (broken 5s,
It is spaced 5s), after crushing, 4 DEG C, 12000r/min centrifugation 15min, supernatant is crude enzyme liquid, aforesaid liquid fermentative medium formula
Percentage is：Glucose 2.0, yeast extract 0.5, Chen Haishui are prepared, and pH is natural；
(2) ammonium sulfate precipitation：6 parts of 25ml crude enzyme liquids are prepared, the cold ammonium sulfate of solid is added thereto respectively, makes its saturated solution dense
Degree difference 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 4 DEG C of 16h that saltout, the dissolving of pH7.8 phosphate buffers
Protein precipitation, determine low-temperature superoxide dismutase activity highest ammonium sulfate precipitation condition；
(3) it is concentrated by ultrafiltration：It will saltout and complete sample and be transferred to molecular cut off 10KDa super filter tubes by pretreatment, 4 DEG C,
6000r/min, ultrafiltration 30min, remove SO4 2-With part small molecular protein, liquid-transfering gun carefully takes out concentrate, and 4 DEG C, preservation is standby
With being partially soluble in phosphate buffer, determine low-temperature superoxide dismutase enzyme activity, calculate protein recovery；
(4) Sephadex G-100 column chromatographies：Sephadex G-100 gel columns are balanced with pH7.8 phosphate buffers, treat baseline
After balance, enzyme liquid adds the equilibrated Sephadex G-100 gel columns (Ф 1 of pH7.8 phosphate buffers after taking 2ml ultrafiltration
.6cm × 60cm), regulation sets automatic collector, and often pipe collects 2ml or so, according to the eluting peak on display, it is determined that containing low
Warm superoxide dismutase is respectively managed, and is collected and is determined its enzyme activity, and merging is respectively managed containing enzyme activity, and 4 DEG C, preservation is standby；
(5) it is spray-dried：The parameter of spray drying is arranged to 110 DEG C of inlet temperature, outlet temperature is 60 DEG C, pressure position 2kgf/
cm2, flow rate of liquid 5mL/min, trehalose in the protective agent in spray drying, sucrose, the dosage of sodium alginate for 2% ~
5%, the dosage of soluble starch is 6% ~ 8%, protectant total dosage control obtains low temperature superoxides within 10%
Mutase.
Crude extract from carthamus tinctorius L. with water, fruit of Chinese wolfberry water extract, Ligusticum wallichii water extract preparation method are as follows：
Chinese medicine（Safflower, the fruit of Chinese wolfberry and Ligusticum wallichii）20g is weighed, adds 400mL deionized waters immersion 24h, moderate heat boils 3h, filtered,
Filter residue adds 200mL deionized waters and extracted 1 time with same method, merges 2 filtrates and is concentrated into 100mL, that is, obtains quality
Concentration is 0.2g/mL (20%) Chinese herbal medicine Aqueous extracts, puts 4 DEG C and saves backup.
The preparation method of jujube concentrate is as follows：
Jujube dry product is taken, 85 DEG C are heated in steam copper and is kept for 15 minutes, the jujube after heating is ground into mud；To after crushing
Jujube paste add pectase, after neutral proteinase, cellulase are sufficiently mixed, mixture temperature is maintained at 25 DEG C of fermentations 1.5
Hour；The jujube material that ferment treatment is crossed is placed in pure water and soaked, pure coolant-temperature gage is maintained at 27 DEG C, soak time 2.5
Hour, do not stop to stir mixture in immersion process；The mixture of the jujube of immersion is filtered in filter, obtains Chinese date juice；
And concentrate Chinese date juice in vacuum decker 45 minutes, Chinese date juice is maintained at 27 DEG C；Finally inspissated juice is added in autoclave
Heat keeps being sterilized for 5 ~ 10 minutes to 90 DEG C；Jujube inspissated juice after sterilizing is cooled into 4 DEG C to save backup, it is last obtained
The mass concentration of jujube inspissated juice is 80%.
The oral liquid is used for 5 slight enteron aisle ulcers, taken 20 days, 3 patients have mitigates disease in various degree
Shape, appetite enhancing, pain mitigate.Undergone according to patient, integrated survey is shown, the oral liquid has a certain degree of intestines
Road antioxidation.
Embodiment 2
The present embodiment selection marine low temperature SOD, crude extract from carthamus tinctorius L. with water, fruit of Chinese wolfberry water extract, Ligusticum wallichii water extract, jujube concentration
Liquid is prepared by following weight proportion：Marine low temperature SOD：3 parts, crude extract from carthamus tinctorius L. with water：1 part, fruit of Chinese wolfberry water extract：1.5
Part, Ligusticum wallichii water extract：1.5 parts, 3 parts of jujube concentrate；Above-mentioned raw materials are mixed, stir vacuum packaging, in postprandial half
Hour directly takes.
Wherein, the preparation method of each raw material used is the same as embodiment 1.
The oral liquid is used for 5 habitual diarrhea patients, taken 20 days, 3 patients have different degrees of alleviation abdomen
Rush down symptom.Undergone according to patient, integrated survey is shown, the oral liquid has a certain degree of improvement gut flora, enhancing
The utilization rate of nutriment, strengthen antioxidation.
Embodiment 3
The present embodiment selection marine low temperature SOD, crude extract from carthamus tinctorius L. with water, fruit of Chinese wolfberry water extract, Ligusticum wallichii water extract, jujube concentration
Liquid is prepared by following weight proportion：Marine low temperature SOD：2 parts, crude extract from carthamus tinctorius L. with water：1.5 part；Fruit of Chinese wolfberry water extract：
1.5 part；Ligusticum wallichii water extract：1 part；3 parts of jujube concentrate；Above-mentioned raw materials are mixed, stir vacuum packaging, in postprandial half
Hour directly takes.
Wherein, the preparation method of each raw material used is the same as embodiment 1.
The oral liquid is used for 5 enteritis patients, taken 20 days, 3 patients have different degrees of alleviation symptom of diarrhea.
Undergone according to patient, integrated survey is shown, the oral liquid has a certain degree of improvement gut flora, strengthens nutriment
Absorption, strengthen antioxidation.
Protection scope of the present invention is not intended to be limited to above example.Those skilled in the art's foundation
Disclosure above and each parameter take scope, and the purpose of the present invention can be achieved, all fall in the scope of protection of the present invention.
Claims (4)
1. a kind of SOD oral liquids of anti-enteron aisle oxidation, it is characterised in that mixed and be made by following raw material：
Marine low temperature SOD：2 ~ 3 parts,
Crude extract from carthamus tinctorius L. with water：1 ~ 2 part,
Fruit of Chinese wolfberry water extract：1 ~ 2 part,
Ligusticum wallichii water extract：1 ~ 2 part,
3 ~ 4 parts of jujube concentrate.
A kind of 2. SOD oral liquids of anti-enteron aisle oxidation according to claim 1, it is characterised in that described marine low temperature
SOD preparation methods specifically include following steps：
(1) preparation of crude enzyme liquid：Sterile working, the sea activated is sticked into Pseudoalteromonas and is inoculated into liquid fermentation medium
In, in 20 DEG C, 160r/min fermentation 48h, zymocyte liquid obtains wet thallus in 4 DEG C, 6000r/min centrifugation 30min, 4 DEG C,
6000r/min centrifuges 30min, and wet thallus dissolves with phosphate buffer, crushed under 480W ultrasonic wave ice baths 30min (broken 5s,
It is spaced 5s), after crushing, 4 DEG C, 12000r/min centrifugation 15min, supernatant is crude enzyme liquid, aforesaid liquid fermentative medium formula
Percentage is：Glucose 2.0, yeast extract 0.5, Chen Haishui are prepared, and pH is natural；
(2) ammonium sulfate precipitation：6 parts of 25ml crude enzyme liquids are prepared, the cold ammonium sulfate of solid is added thereto respectively, makes its saturated solution dense
Degree difference 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 4 DEG C of 16h that saltout, the dissolving of pH7.8 phosphate buffers
Protein precipitation, determine low-temperature superoxide dismutase activity highest ammonium sulfate precipitation condition；
(3) it is concentrated by ultrafiltration：It will saltout and complete sample and be transferred to molecular cut off 10KDa super filter tubes by pretreatment, 4 DEG C,
6000r/min, ultrafiltration 30min, remove SO4 2-With part small molecular protein, liquid-transfering gun carefully takes out concentrate, and 4 DEG C, preservation is standby
With being partially soluble in phosphate buffer, determine low-temperature superoxide dismutase enzyme activity, calculate protein recovery；
(4) Sephadex G-100 column chromatographies：Sephadex G-100 gel columns are balanced with pH7.8 phosphate buffers, treat baseline
After balance, enzyme liquid adds the equilibrated Sephadex G-100 gel columns (Ф 1 of pH7.8 phosphate buffers after taking 2ml ultrafiltration
.6cm × 60cm), regulation sets automatic collector, and often pipe collects 2ml or so, according to the eluting peak on display, it is determined that containing low
Warm superoxide dismutase is respectively managed, and is collected and is determined its enzyme activity, and merging is respectively managed containing enzyme activity, and 4 DEG C, preservation is standby；
(5) it is spray-dried：The parameter of spray drying is arranged to 110 DEG C of inlet temperature, outlet temperature is 60 DEG C, pressure position 2kgf/
cm2, flow rate of liquid 5mL/min, trehalose in the protective agent in spray drying, sucrose, the dosage of sodium alginate for 2% ~
5%, the dosage of soluble starch is 6% ~ 8%, protectant total dosage control obtains low temperature superoxides within 10%
Mutase.
A kind of 3. SOD oral liquids of anti-enteron aisle oxidation according to claim 1, it is characterised in that described safflower water extraction
Thing, fruit of Chinese wolfberry water extract, Ligusticum wallichii water extract preparation method is taken to specifically include following steps：
Chinese medicine（Safflower, the fruit of Chinese wolfberry and Ligusticum wallichii）20g is weighed, adds 400mL deionized waters immersion 24h, moderate heat boils 3h, filtered,
Filter residue adds 200mL deionized waters and extracted 1 time with same method, merges 2 filtrates and is concentrated into 100mL, that is, obtains quality
Concentration is 0.2g/mL (20%) Chinese herbal medicine Aqueous extracts, puts 4 DEG C and saves backup.
A kind of 4. SOD oral liquids of anti-enteron aisle oxidation according to claim 1, it is characterised in that described jujube concentration
Liquid and preparation method thereof specifically includes following steps：
Jujube dry product is taken, 85 DEG C are heated in steam copper and is kept for 15 minutes, the jujube after heating is ground into mud；To after crushing
Jujube paste add pectase, after neutral proteinase, cellulase are sufficiently mixed, mixture temperature is maintained at 25 DEG C of fermentations 1.5
Hour；The jujube material that ferment treatment is crossed is placed in pure water and soaked, pure coolant-temperature gage is maintained at 27 DEG C, soak time 2.5
Hour, do not stop to stir mixture in immersion process；The mixture of the jujube of immersion is filtered in filter, obtains Chinese date juice；
And concentrate Chinese date juice in vacuum decker 45 minutes, Chinese date juice is maintained at 27 DEG C；Finally inspissated juice is added in autoclave
Heat keeps being sterilized for 5 ~ 10 minutes to 90 DEG C；Jujube inspissated juice after sterilizing is cooled into 4 DEG C to save backup.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109770133A (en) * | 2019-03-11 | 2019-05-21 | 大兴安岭冰莓庄园生物科技发展有限公司 | The preparation method of wild Cranberry health beverages |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072090A (en) * | 1991-11-05 | 1993-05-19 | 武汉市辛安渡制药总厂 | The preparation method of SOD oral liquid |
| CN103919839A (en) * | 2014-04-18 | 2014-07-16 | 东北林业大学 | Application of Trichosanthes kirilowii, safflower and hemlock parsley for relieving oxidative stress injury to drosophila intestinal tract |
| CN106987565A (en) * | 2017-05-22 | 2017-07-28 | 大连大学 | A kind of marine low temperature superoxide dismutase extraction and separation process |
| CN107048394A (en) * | 2017-03-30 | 2017-08-18 | 大连大学 | It is a kind of that there are krill polypeptide formulations of improvement sleep function and preparation method thereof |
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2017
- 2017-11-10 CN CN201711103093.0A patent/CN107865423A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072090A (en) * | 1991-11-05 | 1993-05-19 | 武汉市辛安渡制药总厂 | The preparation method of SOD oral liquid |
| CN103919839A (en) * | 2014-04-18 | 2014-07-16 | 东北林业大学 | Application of Trichosanthes kirilowii, safflower and hemlock parsley for relieving oxidative stress injury to drosophila intestinal tract |
| CN107048394A (en) * | 2017-03-30 | 2017-08-18 | 大连大学 | It is a kind of that there are krill polypeptide formulations of improvement sleep function and preparation method thereof |
| CN106987565A (en) * | 2017-05-22 | 2017-07-28 | 大连大学 | A kind of marine low temperature superoxide dismutase extraction and separation process |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109770133A (en) * | 2019-03-11 | 2019-05-21 | 大兴安岭冰莓庄园生物科技发展有限公司 | The preparation method of wild Cranberry health beverages |
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