CN107821731B

CN107821731B - Soybean enzymolysis protein spraying liquid and preparation method thereof

Info

Publication number: CN107821731B
Application number: CN201711087323.9A
Authority: CN
Inventors: 刘汝萃; 范书琴; 马军; 许建; 李国新
Original assignee: Shandong Yuwang Ecological Food Industry Co Ltd
Current assignee: Shandong Yuwang Ecological Food Industry Co Ltd
Priority date: 2017-11-07
Filing date: 2017-11-07
Publication date: 2021-03-26
Anticipated expiration: 2037-11-07
Also published as: CN107821731A

Abstract

The invention belongs to the technical field of soybean protein production, and particularly relates to a soybean enzymatic protein spray coating liquid and further discloses a preparation method thereof. The soybean enzymolysis protein spray coating liquid disclosed by the invention takes phospholipid and sucrose fatty acid ester as main components, and meanwhile, the process water subjected to reverse osmosis filtration replaces soybean oil to be used as a solvent, so that the dispersion performance and the dissolving speed of the obtained soybean enzymolysis protein are greatly improved compared with the oil phase spray coating liquid taking the soybean oil as the solvent in the prior art.

Description

Soybean enzymolysis protein spraying liquid and preparation method thereof

Technical Field

The invention belongs to the technical field of soybean protein production, and particularly relates to a soybean enzymatic protein spray coating liquid and further discloses a preparation method thereof.

Background

The soybean enzymolysis protein is a high-quality protein which is prepared by using soybeans as raw materials, performing conversion treatment by using a bioengineering technology and drying at high temperature and is suitable for the digestive physiological characteristics of young animals. The protein content of the soybean enzymolysis protein is more than 90 percent, the amino acid types are nearly 20, and the soybean enzymolysis protein contains various amino acids essential to human bodies. The soybean enzymolysis protein is rich in nutrition, and meets the special requirements of young animals, carnivorous animals and aquatic animals on the quality of the protein.

In the soybean enzymolysis protein, most of raffinose and stachyose are removed, so that the antigenicity of glycinin is eliminated, and other anti-nutritional factors such as non-starch polysaccharide, phytic acid and the like are also treated, so that the anti-nutritional factors in the soybean enzymolysis protein are lower; moreover, because the original spherical structure of the soybean protein is destroyed, the peptide chain is in a stretched state, and the internal enzyme action site is fully exposed, the digestibility is improved; in addition, the soybean enzymolysis protein has unique fragrance, is beneficial to improving the palatability, and makes animals prefer to eat, and the soybean enzymolysis protein is easy to digest, so that the feed intake is draft and lasting.

In the prior art, a typical production process of soybean enzymatic protein is an alkali-soluble acid-precipitation method, i.e., soybean meal is soaked in weak alkali liquor for about 40 minutes, so that protein is dissolved in the weak alkali liquor, a separator is used for separating and removing insoluble fibers, a solution containing protein is left, a flocculating agent (generally food-grade hydrochloric acid) is added into the protein solution, the protein is precipitated and recovered, then the recovered protein is subjected to enzyme treatment, and the obtained protease hydrolysate is dried to obtain the soybean enzymatic protein. However, the soybean enzymatic protein powder obtained through the above preliminary processing only has general properties of soybean enzymatic protein, and for many application fields, the properties of the soybean enzymatic protein powder cannot meet processing requirements, and the dried enzymatic protein powder needs to be sprayed to obtain the soybean enzymatic protein suitable for the performance requirements of different processing fields.

Disclosure of Invention

Therefore, the technical problem to be solved by the invention is to provide a soybean enzymatic protein spray coating liquid to further improve the dissolving performance of soybean enzymatic protein.

In order to solve the technical problems, the soybean enzymatic protein spraying liquid comprises the following components in parts by weight: 5-10 parts of phospholipid, 1-4 parts of sucrose fatty acid ester and 20-30 parts of water.

Preferably, the spraying liquid comprises the following components in parts by weight: 6-9 parts of phospholipid, 2-3 parts of sucrose fatty acid ester and 22-28 parts of water.

Preferably, the spraying liquid comprises the following components in parts by weight: 7.8 parts of phospholipid, 2.4 parts of sucrose fatty acid ester and 25.8 parts of water.

The water is process water filtered by reverse osmosis.

The invention also discloses a method for preparing the soybean enzymolysis protein spraying liquid, which comprises the steps of heating a selected amount of water, sequentially adding a selected amount of phospholipid and sucrose fatty acid ester, and uniformly mixing to obtain the soybean enzymolysis protein spraying liquid.

The heating temperature of the water is controlled to be 60-70 ℃.

Further comprising the step of shearing the mixture with a high-speed shear pump while adding the phospholipid and the sucrose fatty acid ester.

Also comprises the step of preserving the obtained spraying liquid at 70 ℃.

The invention also discloses the application of the soybean enzymolysis protein spraying liquid in the production process of the soybean enzymolysis protein, and the spraying amount of the spraying liquid is 1-3 per mill based on the dry basis of the soybean enzymolysis protein.

And the spraying liquid is sprayed by a metering pump through a two-fluid spray gun.

The spraying liquid is kept warm and stirred in the spraying process so as to prevent the spraying liquid from demixing and precipitating.

The soybean enzymolysis protein spray coating liquid disclosed by the invention takes phospholipid and sucrose fatty acid ester as main components, and meanwhile, the process water subjected to reverse osmosis filtration replaces soybean oil to be used as a solvent, so that the dispersion performance and the dissolving speed of the obtained soybean enzymolysis protein are greatly improved compared with the oil phase spray coating liquid taking the soybean oil as the solvent in the prior art.

According to the soybean enzymolysis protein spraying liquid, process water subjected to reverse osmosis filtration replaces soybean oil to serve as a solvent, and detection shows that the total number of bacterial colonies in the process water is less than 500cfu/g, escherichia coli is less than 3.0cfu/g, and mold and yeast are less than 50cfu/g, so that the breeding of microorganisms is avoided, and the dispersion speed of protein powder is increased.

Drawings

In order that the present disclosure may be more readily and clearly understood, the following detailed description of the present disclosure is provided in connection with specific embodiments thereof and the accompanying drawings, in which,

FIG. 1 is a schematic view of the present invention utilizing a two-fluid spray gun for spraying.

Detailed Description

Example 1

The soybean enzymatic protein spraying liquid comprises the following components: 5kg of phospholipid, 4kg of sucrose fatty acid ester and 20kg of process water.

The method for preparing the soybean enzymatic protein spraying liquid comprises the following steps: heating a selected amount of water to 60-70 ℃, sequentially adding a selected amount of phospholipid and sucrose fatty acid ester, simultaneously shearing by using a high-speed shearing pump until the solution is uniformly dissolved to obtain the spraying liquid, and placing the spraying liquid in a 70 ℃ heat-preservation water bath interlayer for heat preservation and preservation.

Example 2

The soybean enzymatic protein spraying liquid comprises the following components: 10kg of phospholipid, 1kg of sucrose fatty acid ester and 30kg of process water.

The preparation method of the soy protein hydrolysate spray coating solution is the same as that of example 1.

Example 3

The soybean enzymatic protein spraying liquid comprises the following components: 6kg of phospholipid, 3kg of sucrose fatty acid ester and 22kg of process water.

Example 4

The soybean enzymatic protein spraying liquid comprises the following components: 9kg of phospholipid, 2kg of sucrose fatty acid ester and 28kg of process water.

Example 5

The soybean enzymatic protein spraying liquid comprises the following components: 7.8kg of phospholipid, 2.4kg of sucrose fatty acid ester and 25.8kg of process water.

Comparative example 1

The soybean enzymolysis protein spraying solution comprises the following components: 5.4kg of phospholipid, 1.8kg of sucrose fatty acid ester and 4.8kg of soybean oil.

Application example

The spraying process of the spraying liquid is that a two-fluid spray gun which is commonly used in the prior art is used for spraying, namely the spraying is carried out according to the using method of the two-fluid spray gun. As shown in fig. 1, a schematic diagram of a spraying process of a general two-fluid spray gun is given, liquid (spraying liquid) is conveyed to a nozzle through a liquid flow channel under the action of pressure, compressed air reaches the nozzle through a gas flow channel, the compressed air and the gas flow channel are mixed at the nozzle to generate an atomized body (circular spray shape), the atomized body can keep a regular sector area within a distance 'B', the angle is as large as 'a', when the distance 'D' is exceeded, the atomized body becomes turbulent flow, the shape changes, A, B, D parameters in the drawing can change according to different types of two-fluid nozzles, and the spraying liquid can be atomized uniformly and any type of nozzle can be selected.

As shown in the schematic spray coating diagram of FIG. 1, commercially available soy protein enzymatic hydrolysate of the prior art was spray-coated with the spray coating solutions of examples 1-5 and comparative example 1 by a metering pump through a two-fluid spray gun to obtain corresponding protein samples, respectively. With soyabean enzymolysis protein dry basis counts, controls the spraying volume of spraying liquid is 1-3 thousandths to it is right at the spraying in-process the spraying liquid keeps warm continuously, and the stirring tooth continuously stirs in the formulation jar, prevents that spraying liquid from layering and settling.

Experimental examples dispersibility test

100 plus or minus 0.1g of distilled water at 50 to 55 ℃ is added into a 250ml beaker, 5.0 plus or minus 0.1g of a protein sample (obtained by spraying the spray coating liquid prepared in examples 1 to 5 and comparative example, respectively) is weighed by an electronic balance and poured into the beaker, clockwise stirring is carried out by a glass rod attached to the wall of the beaker, and timing is started, the stirring speed is controlled to be about 3 circles/s, and the required time is recorded when no dry powder exists, namely the dispersion speed.

The dispersion rate of the soy protein samples obtained by the spray coating treatment described in examples 1-5 was determined to be 10-15s, while the dispersion rate of the soy protein samples obtained by the spray coating treatment described in the comparative example was determined to be 25-29 s. Therefore, compared with common oily spraying liquid in the prior art, the aqueous phase spraying liquid provided by the invention greatly improves the dissolving speed of protein.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.

Claims

1. The soybean enzymolysis protein spraying liquid is characterized by comprising the following components in parts by weight: 5-10 parts of phospholipid, 1-4 parts of sucrose fatty acid ester and 20-30 parts of water.

2. The soy protein hydrolysate spray coating liquid according to claim 1, wherein the spray coating liquid comprises the following components in parts by weight: 7.8 parts of phospholipid, 2.4 parts of sucrose fatty acid ester and 25.8 parts of water.

3. The soy protein hydrolysate spray coating liquid according to claim 1 or 2, wherein the water is process water filtered by reverse osmosis.

4. A method for preparing the soybean enzymatic protein spray coating liquid of any one of claims 1 to 3, which comprises the steps of heating a selected amount of water, sequentially adding a selected amount of the phospholipid and the sucrose fatty acid ester, and uniformly mixing to obtain the soybean enzymatic protein spray coating liquid.

5. The method for preparing the soy protein hydrolysate spray coating liquid according to claim 4, wherein the heating temperature of water is controlled to be 60-70 ℃.

6. The method for preparing the soy protein hydrolysate spray coating liquid as claimed in claim 5, further comprising the step of performing a shearing treatment with a high-speed shearing pump while adding the phospholipid and the sucrose fatty acid ester.

7. The method for preparing the soy protein hydrolysate spray coating liquid according to any one of claims 4 to 6, further comprising the step of preserving the obtained spray coating liquid at 70 ℃.

8. The use of the soy protein hydrolysate spray coating solution of any one of claims 1 to 3 in the soy protein hydrolysate production process, wherein the spray coating amount of the spray coating solution is 1 to 3 per thousand on a dry basis of the soy protein hydrolysate.

9. Use according to claim 8, wherein the spraying liquid is sprayed by a metering pump through a two-fluid spray gun.

10. Use according to claim 8 or 9, wherein the spraying liquid is kept warm and stirred during spraying to prevent the spraying liquid from demixing and settling.