CN107817218B - Full-automatic microscope continuous detection system - Google Patents
Full-automatic microscope continuous detection system Download PDFInfo
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- CN107817218B CN107817218B CN201711083630.XA CN201711083630A CN107817218B CN 107817218 B CN107817218 B CN 107817218B CN 201711083630 A CN201711083630 A CN 201711083630A CN 107817218 B CN107817218 B CN 107817218B
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- 238000001514 detection method Methods 0.000 title claims abstract description 38
- 238000012546 transfer Methods 0.000 claims abstract description 67
- 239000005357 flat glass Substances 0.000 claims abstract description 57
- 238000005070 sampling Methods 0.000 claims abstract description 50
- 230000007246 mechanism Effects 0.000 claims abstract description 33
- 238000003825 pressing Methods 0.000 claims description 31
- 230000033001 locomotion Effects 0.000 claims description 15
- 230000005540 biological transmission Effects 0.000 claims description 13
- 239000002699 waste material Substances 0.000 claims description 12
- 238000011084 recovery Methods 0.000 claims description 11
- 239000011521 glass Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 238000007689 inspection Methods 0.000 claims description 5
- 230000007704 transition Effects 0.000 claims description 3
- 238000012545 processing Methods 0.000 abstract description 4
- 230000001360 synchronised effect Effects 0.000 abstract description 3
- 238000007790 scraping Methods 0.000 abstract 2
- 230000003287 optical effect Effects 0.000 description 7
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
Abstract
The invention discloses a full-automatic microscope continuous detection system, which comprises a microscope detection device, a control system, a plain film transfer device, a doctor blade transfer device, a sampling and sample adding driving device and an auxiliary device. The flat sheet transfer device is used for moving the clamping mechanism along the longitudinal direction to take out the flat glass slide from the clean sheet supply device, and after the operations of sample adding and strickling, the flat glass slide is moved to the area of the microscope detection device in a transverse mode to detect and record samples on the flat glass slide through the microscope detection device. The scraping sheet transfer device and the sampling and sample adding driving device are fixed on the same combined frame and move transversely synchronously. The auxiliary devices are sequentially arranged on one side of the combined rack. The invention realizes a series of coordinated synchronous automatic operation functions of sampling, sample adding, scraping, smear, transfer detection and resetting. And the computer for image processing and detection and identification of the detected object realizes full-automatic detection and identification of the detected object.
Description
The technical field is as follows:
the invention belongs to the technical field of medical microscopic detection equipment, and particularly relates to a full-automatic continuous detection system of a microscope.
Background art:
at present, microscopes have been widely used in various fields such as medical treatment, scientific research, and teaching, especially in the biomedical field. However, the operation and use of the microscope, the storage, processing, printing and the like of the image information are not very convenient at present, and most microscopes can only be operated manually. In recent years, there has been a great progress in the automation of microscopes, but the degree of automation of microscopes is not so high.
Chinese patent publication No. CN1553241A discloses an optical automatic microscope, which includes a main microscope composed of an eyepiece, an objective lens, an objective table, a condenser lens, and an illumination light source, wherein the objective table is provided with a horizontal transverse axis motor, a horizontal longitudinal axis motor, and other components, although an electric converter is provided, the objective table is mainly improved with respect to a moving structure and a control part of a top of the main microscope. The advantage of the invention is that it enables autofocus auto-scanning of the image. The defect is that the automation degree is not high, and the automatic movement of the slide glass of the object to be detected can not be realized.
Chinese patent publication No. CN 105988209 a discloses a microscope-based full-automatic detection system, which includes a mechanical system mounted on a base, an optical microscope, a control system for controlling a stepping motor of the mechanical system, a CCD camera mounted on a frame of the optical microscope, and a computer located outside the base. The mechanical system moves the smear of the object to be detected under the optical microscope, light emitted by a light source of the optical microscope passes through the smear of the object to be detected, the light is focused and imaged on an electronic eyepiece of the CCD camera through an objective lens, and the CCD camera converts an imaged optical signal into an electric signal and then transmits the electric signal to the computer for image processing and detection. The patent document still aims at the technical improvement of the microscope in the aspect of CCD camera imaging, although the automation degree is improved to a certain extent, and the labor cost is reduced, the aspects of sample adding, smear and the like still need manual participation, and the coordination and synchronization operation of automatic inclined slide and sample adding cannot be realized, so the purpose of full-automatic detection cannot be realized.
The invention content is as follows:
the invention provides a continuous detection system capable of realizing a full-automatic microscope, aiming at the problems that the existing microorganism culture or detection equipment has a simple structural form and cannot realize comprehensive automatic detection.
The technical scheme is as follows: a full-automatic microscope continuous detection system comprises a microscope detection device and a control system which are arranged on a bottom plate, a sampling and sample adding device, a sample and suction nozzle box, an inclined slide supply device, an inclined slide transfer device, a flat slide supply device and a slide transfer device.
The inclined slide transfer device and the sampling and sample adding device are fixed on the same combined frame and drive synchronous transverse movement.
The inclined slide transfer device comprises a first driving mechanism which can enable a first lifting slide block to move vertically; the lower end of the first lifting sliding block is provided with an elastically opened clamping nozzle, a first sliding shaft is arranged on a nozzle piece capable of swinging of the clamping nozzle, and a guide sliding structure which enables the first sliding shaft to move downwards and then to be close to the combined frame so as to enable the clamping nozzle to be closed is arranged on one side of the combined frame. And meanwhile, a downward pressing pulley is arranged at the lower end of the first lifting slide block through an extension rod.
The inclined slide supply device comprises a shaft support and an inclined support which are fixed on the bottom plate, the upper end of the shaft support is hinged with an L-shaped swing rod through a rotating shaft, and one end of the L-shaped swing rod is in transmission connection with the lifting slide block; an inclined push plate capable of translating along the inclined support is installed on one side of the inclined support and is in transmission connection with the other end of the L-shaped swing rod; an inclined slide stacking box is fixed on the inclined support, the lower side of the inclined push plate is located at the bottom of the inclined slide stacking box, and when the inclined push plate translates upwards, the inclined slide at the bottom layer of the inclined slide stacking box can be pushed out upwards, so that the inclined slide at the bottom layer is pushed into the position of the clamping mouth.
The flat slide transfer device comprises a longitudinal moving mechanism and a transverse moving mechanism on the basis of the longitudinal moving mechanism, a support is fixed to the lower side of the front end of the transverse moving mechanism, a clamping nozzle with closed elasticity is mounted on the support, a piece taking supporting pressing block is arranged below the flat slide transfer device on the bottom plate, a piece unloading supporting pressing block is arranged at the position where waste pieces are recovered, and the clamping nozzle can be opened after being contacted with the piece taking supporting pressing block and the piece unloading supporting pressing block.
The flat glass slide supply device comprises a flat glass slide stacking box fixed on the base, the lower end of the flat glass slide stacking box is provided with an opening and is provided with a pushing plate capable of translating back and forth, and the pushing plate is connected with a transmission mechanism to realize the process of translating and pushing back and forth; the pushing groove is formed in the upper side of the pushing plate, flat glass slides in the flat glass slide stacking box can fall into the pushing groove, and the flat glass slides are pushed to the clamping mouth through the pushing groove.
The flat glass slide transfer device moves along the longitudinal direction, a clamping mechanism (a clamping mouth) is used for taking a flat glass slide out of the flat glass slide supply device, after the operations of sample adding and strickling are carried out, the flat glass slide transfer device moves to the area of the microscope detection device in a transverse mode, a sample on the flat glass slide is detected and recorded through the microscope detection device, and then the flat glass slide is returned to be unloaded and reset; the inclined slide transfer device and the sampling and sample adding device move synchronously, and the inclined slide transfer device and the sampling and sample adding device are matched with the inclined slide supply device and the slide supply device as well as the waste piece recovery box and the sample and suction nozzle box as follows: (1) when the inclined slide transfer device and the sampling and sample adding device are simultaneously positioned at the upper sides of the inclined slide supply device and the flat slide supply device, the inclined slide transfer device moves downwards to a position B to obtain an inclined slide, and the sampling and sample adding device adds samples to the flat glass slide; (2) when the inclined slide transfer device and the sampling and sample adding device are simultaneously positioned above the flat slide supply device and the waste piece recovery box, the inclined slide transfer device continuously moves the lower part of the inclined slide to a position C and presses one end of a flat glass slide, after the flat glass slide transfer device longitudinally pulls the flat glass slide, a sample on the flat glass slide is scraped, and at the moment, the sampling and sample adding device withdraws a suction nozzle after sample adding; (3) when the inclined slide transfer device and the sampling and sample adding device are simultaneously positioned above the waste piece recovery box and the sample and suction nozzle box, the inclined slide transfer device is moved upwards to a position A to remove the inclined slide, and the sampling and sample adding device is inserted into the suction nozzle and absorbs the sample; the above (1) to (3) are circulated repeatedly.
The flat glass slide transfer device comprises two longitudinal slide ways which are fixed on the bottom plate in parallel, longitudinal slide blocks are respectively arranged on the two longitudinal slide ways, transverse slide ways are fixed on the two longitudinal slide blocks, a longitudinal driving mechanism which enables the longitudinal slide blocks to move on the longitudinal slide ways is arranged, transverse slide blocks are arranged on the transverse slide ways, and a transverse driving mechanism which enables the transverse slide blocks to move on the transverse slide ways is arranged; the front end lower side of the transverse sliding block is fixed with a support, an L-shaped clamping mouth is hinged to the support through a pin shaft, the upper side of the L-shaped clamping mouth extends out of the upper side of the transverse sliding block, and a spring or a torsion spring for keeping the clamping mouth and the transverse sliding block to be pressed tightly is arranged.
The inclined slide transfer device comprises an inclined slide taking lifting motor fixed on a combined frame, a first driving belt wheel is installed on a rotating shaft of the inclined slide taking lifting motor, a first shaft sleeve is fixed on the combined frame, a first vertical rotating shaft is installed in the first shaft sleeve, a first driven belt wheel is installed at the upper end of the first vertical rotating shaft, and the first driving belt wheel and the first driven belt wheel are in belt transmission; the lower end of the first vertical rotating shaft is in threaded connection with a first lifting sliding block, an inclined supporting plate is fixed at the lower end of the first vertical sliding block, an inclined pressing plate is hinged to the lower side of the inclined supporting plate through a pin shaft, and the inclined pressing plate is kept away from the inclined supporting plate under the pressure of a spring or a torsion spring; meanwhile, slideways are fixed on the combined frame in parallel; sliding shafts I are symmetrically arranged on two sides of the inclined pressing plate, and are arranged in corresponding slideways in a matching manner; the slideway comprises an upper section and a lower section which are in smooth transition, wherein the two sections are parallel to the combined rack, but the width d1 from the lower section to the combined rack is smaller than the width d2 from the upper section to the combined rack.
The sampling and sample adding device comprises a sampling lifting motor fixed on the combined frame, a driving belt wheel II is installed on a rotating shaft of the sampling lifting motor, a shaft sleeve II is fixed on the combined frame, a vertical rotating shaft II is installed in the shaft sleeve II, a driven belt wheel II is installed at the upper end of the vertical rotating shaft II, and the driving belt wheel II and the driven belt wheel II are in belt transmission; the lower end of the second vertical rotating shaft is in threaded connection with a second lifting slide block, and a sampling mechanism is fixed at the lower end of the second vertical slide block.
A flexible buffer layer is arranged in the pushing groove.
The invention has the following beneficial effects: based on the existing optical microscope imaging technology and an automatic control system, the invention combines a flat slide transfer device, an inclined slide transfer device, a sampling and sample adding device and other auxiliary devices to realize a series of coordination synchronous automatic operation functions of sampling, sample adding, inclined slide extracting, smear, transfer detection and reset. And the computer for image processing and detection and identification of the detected object realizes full-automatic detection and identification of the detected object. The invention has high automation degree, greatly simplifies the detection procedure, reduces the problems of labor cost and misoperation, improves the detection speed and efficiency and improves the medical detection environment.
Drawings
Fig. 1 is a schematic view of the appearance structure of the present invention.
Fig. 2 is one of the internal structural diagrams of fig. 1.
Fig. 3 is a top view of fig. 2.
Fig. 4 is a schematic view showing the relationship of movement of the flat slide transfer device of fig. 3.
FIG. 5 is a schematic diagram showing the relationship between the slide transfer device and the sample loading device carried by the rack assembly of FIG. 3.
FIG. 6 is a schematic view of a slide operating mechanism.
Fig. 7 is a side view of the structure of fig. 6.
Fig. 8 is a schematic view of a flat slide transfer mechanism.
Fig. 9 is a schematic top view of the structure of fig. 8.
Fig. 10 is a side view of the structure of fig. 8.
Fig. 11 is a second schematic diagram of the internal structure of fig. 1.
FIG. 12 is a schematic view of a flat slide buffer configuration.
FIG. 13 is a schematic view showing an initial state in which the slide transfer device cooperates with the slide supply device.
Fig. 14 is a schematic view showing an end state of the slide transfer device in cooperation with the slide supply device.
FIG. 15 is a schematic view showing the state in which the flat slide transfer device and the flat slide supply device are engaged with each other.
Fig. 16 is a perspective view of fig. 15.
In the figure, reference numeral 1 is a housing, 2 is a base plate, 3 is a flat slide transfer device, 301 is a lateral slide, 302 is a right-side longitudinal slide, 303 is a right-side longitudinal slide, 304 is a left-side longitudinal slide, 305 is a left-side longitudinal slide, 306 is a lateral movement driving motor, 307 is a lateral movement driving pulley, 308 is a lateral movement driven pulley, 309 is a lateral belt, 310 is a longitudinal movement driving motor, 311 is a longitudinal movement driving pulley, 312 is a first fixing plate, 313 is a longitudinal movement driven pulley, 314 is a lateral slide, 315 is a support, 316 is a clamping mouth, 317 is a pin shaft, 318 is a first flexible cushion block, 319a is a taking piece supporting press block, 319b is a taking piece supporting press block, 320 is a longitudinal belt, 321 is a second fixing plate, 4 is a combined frame lateral movement driving device, 401 is a fixing vertical plate and a lateral slide, 402 is an L-shaped combined frame, 403 is a driving motor, 404 is a driving pulley, 405 is a driven pulley, 406 is a belt, 406, 501 is a sampling lifting motor, 502 is a driving pulley II, 503 is a driven pulley II, 504 is a belt, 505 is a shaft sleeve II, 506 is a vertical rotating shaft II, 507 is a lifting slide block II, 508 is a fixed seat, 509 is a sampling mechanism, 510 is a suction nozzle, 6 is an inclined slide transfer device, 601 is an inclined slide taking lifting motor, 602 is a driving pulley I, 603 is a driven pulley I, 604 is a belt, 605 is a vertical rotating shaft I, 606 is a slide rail bracket, 607 is a slide rail, 608 is a lifting slide block I, 609 is a slide shaft I, 610 is an inclined pressing plate, 611 is an inclined supporting plate, 612 is a shaft sleeve I, 613 is a pin shaft, 614 is a flexible supporting block II, 615 is an extension rod, 616 is a pressing pulley, 7 is an inclined slide supply device, 701 is a shaft seat, 702 is a rotating shaft, 703 is an L-shaped swing rod, 704 is a sliding inclined plane, 705 is a guide hole, 706 is an inclined support, 707 is a sliding shaft II, 708 is an inclined pushing plate, and 709 is an inclined slide, 8 is a flat slide supply device, 801 is a flat slide stacking box, 802 is a push plate, 803 is a push groove, 804 is a cushion pad, 805 is a threaded slide block, 806 is a screw rod, and 807 is a motor; 9 is a sample and suction nozzle box, 10 is a waste chip recovery box, 11 is a circuit board, 12 is a microscope detection device, 13 is a flat glass slide, and 14 is an inclined glass slide.
The specific implementation mode is as follows:
the appearance of the fully automatic microscope continuous inspection system is shown in fig. 1, and comprises a housing 1 and an operation window. As shown in fig. 2, the main components of the internal structure are a flat slide transfer device 3, a flat slide supply device 8, an oblique slide transfer device 6, and an oblique slide supply device, which are provided on a base plate 2. And a sampling and sample adding device 5, a waste chip recovery box 10, a sample and suction nozzle box 9, a microscope detection device 12, a circuit board 11, a control system and the like.
As can be seen from fig. 4, the flat glass slide transfer device 3 is moved in the longitudinal direction, the flat glass slide 13 is taken out from the flat glass slide supply device 8 by the gripper mechanism, and after the operations of sample loading and leveling, the flat glass slide is moved to the area of the microscope detection device 12, the sample on the flat glass slide 13 is detected and recorded by the microscope detection device 12, and then the flat glass slide 13 is returned and unloaded and reset.
As can be seen from fig. 9, 10 and 11, the flat slide transfer device 3 includes two longitudinal slides fixed in parallel on the base plate 2, two ends of the slide are respectively mounted on the first fixing plate and the second fixing plate, and a longitudinal driving mechanism for moving the longitudinal slide block on the longitudinal slide is provided. Specifically, a longitudinal driven belt wheel is installed on the first fixing plate, a longitudinal driving motor is installed on the second fixing plate, a longitudinal driving belt wheel is installed on a rotating shaft of the longitudinal driving motor, and annular longitudinal belts are sleeved on the longitudinal driving belt wheel and the longitudinal driven belt wheel.
Two longitudinal slide blocks are respectively arranged on the longitudinal slide ways, and the longitudinal slide blocks on two sides are respectively fixed with the corresponding longitudinal belt together, so that the longitudinal slide blocks can be driven to move when the longitudinal belt rotates.
Meanwhile, a transverse slide way is fixed on the two longitudinal slide blocks, and a transverse driving mechanism which enables the transverse slide blocks to move on the transverse slide way is arranged. Specifically, a transverse moving driving motor and a transverse driven belt wheel are respectively arranged on the two longitudinal sliding blocks, a transverse driving belt wheel is arranged on a rotating shaft of the transverse moving driving motor, and an annular transverse belt is sleeved on the transverse driving belt wheel and the transverse driven belt wheel.
The transverse sliding block is arranged on the transverse sliding way and fixed with the transverse belt, so that the transverse sliding block can be driven to move when the transverse belt rotates.
As can be seen from figure 11, a support is fixed on the lower side of the front end of the transverse sliding block, an L-shaped clamping mouth is hinged on the support through a pin shaft, the upper side of the L-shaped clamping mouth extends out of the upper side of the transverse sliding block, and a spring or a torsion spring for keeping the clamping mouth and the transverse sliding block to be pressed is arranged. When the longitudinal slide block is pushed forward, the transverse slide way and the transverse slide block are driven to move forward, and when the L-shaped clamping nozzle contacts the sheet taking support pressing block 319a or the sheet unloading support pressing block 319b, the L-shaped clamping nozzle is automatically opened so as to take the flat glass carrier sheet 13 or unload the flat glass carrier sheet 13. The blade-taking support pressing block 319a and the blade-removing support pressing block 319b are identical in structure and function, but different in lateral position. After the horizontal sliding block moves along the horizontal direction, the horizontal sliding block can be selectively contacted with the piece-taking supporting pressing block 319a or the piece-unloading supporting pressing block 319b to realize the function.
As shown in fig. 15 and 16, the flat slide supply device includes a flat slide stacking box fixed on the base, the lower end of the flat slide stacking box is open and provided with a push plate capable of moving back and forth, and the push plate is connected with the transmission mechanism to realize the process of moving back and forth; the upper side of the pushing plate is provided with a pushing groove, and the flat glass slides in the flat glass slide stacking box can fall into the pushing groove. The bottom of the pushing plate is fixedly provided with a nut sliding block, a threaded hole of the nut sliding block is internally provided with a screw rod, the screw rod is connected with a rotating shaft of the motor, and the nut sliding block is also provided with a horizontal hole and is sleeved with a translation guide rod fixed on the base.
As can be seen from fig. 5 and 6, the slide transfer device 6 and the sampling and sample-adding device 5 are fixed on the same L-shaped rack 402 and move transversely synchronously. The combination rack transverse moving driving device 4 for driving the L-shaped combination rack 402 to move transversely comprises a group of fixed vertical plates and transverse sliding ways which are arranged on the bottom plate 2, a driving motor is fixed at one end of each fixed vertical plate, a driving belt wheel is installed on a rotating shaft of the driving motor, a driven belt wheel is installed at the other end of each fixed vertical plate, belts are wound on the driving belt wheel and the driven belt wheel, the L-shaped combination rack 402 is installed on the transverse sliding ways and can slide, and the L-shaped combination rack 402 is fixed on the belts. Therefore, when the driving motor drives the belt to rotate, the L-shaped combination rack 402 can be driven to perform a transverse reciprocating motion.
As can be seen from fig. 13 and 14, the inclined slide supply device comprises a shaft support and an inclined support fixed on the bottom plate, the upper end of the shaft support is hinged with an L-shaped swing rod through a rotating shaft, one end of the L-shaped swing rod is provided with a sliding inclined surface, the sliding inclined surface is matched with the lower pressing pulley to slide, and the other end of the L-shaped swing rod is provided with a guide hole; an inclined push plate capable of translating along the inclined support is installed on one side of the inclined support, a second sliding shaft is installed on one side of the inclined push plate, and the second sliding shaft is sleeved in the guide hole in a matching mode; an inclined slide stacking box is fixed on the inclined support, and the lower side of the inclined push plate is positioned at the bottom of the inclined slide stacking box.
The sample and nozzle box 9, the microscope detection device 12 and the control system in the present invention are implemented by using the known technology. Wherein the microscope detection device 12 comprises the processes of dyeing, drying, photographing, microscopic detection and the like for the flat glass slide.
Referring to fig. 5, the inclined slide transfer device 6 and the sampling and sample-adding device 5 and the auxiliary device include the following matching relations: (1) when the inclined slide transfer device 6 and the sampling and sample adding device 5 are simultaneously positioned at the upper sides of the inclined slide supply device 7 and the glass slide supply device 8, the inclined slide transfer device 6 moves downwards to a position B to take an inclined slide, and the sampling and sample adding device 5 adds samples to the flat glass slide 13; (2) when the inclined slide transfer device 6 and the sampling and sample adding device 5 are simultaneously positioned above the flat slide supply device 8 and the waste piece recovery box 10, the inclined slide transfer device 6 continuously moves an inclined slide downwards to a position C and presses one end of a flat glass slide 13, after the flat glass slide transfer device 3 pulls the flat glass slide 13 along the longitudinal direction, a sample on the flat glass slide 13 is scraped (smeared), and at the moment, a suction nozzle after sample adding is withdrawn by the sampling and sample adding device 5; (3) when the oblique slide transfer device 6 and the sampling and sample adding device 5 are simultaneously positioned above the waste piece recovery box 10 and the sample and suction nozzle box 9, the oblique slide transfer device 6 moves upwards to the position A to remove the oblique slide, and the sampling and sample adding device 5 is inserted with the suction nozzle and sucks the sample. The above (1) to (3) are circulated repeatedly. Where position a is higher than position B, which is higher than position C.
The concrete structure of the sampling and sample adding device 5 is shown in fig. 6, and comprises a sampling lifting motor fixed on a combined frame, wherein a second driving belt wheel is installed on a rotating shaft of the lifting motor, a second shaft sleeve is fixed on the combined frame, a second vertical rotating shaft is installed in the second shaft sleeve, a second driven belt wheel is installed at the upper end of the second vertical rotating shaft, and the second driving belt wheel and the second driven belt wheel are in belt transmission; the lower end of the second vertical rotating shaft is in threaded connection with a second lifting slide block, and a sampling mechanism is fixed at the lower end of the second vertical slide block. The sampling mechanism is a liquid sampling mechanism which is commonly used at present, and the structure and the working principle of the sampling mechanism are not detailed. The process of moving and sucking the sample after installing the suction nozzle at the lower end of the telescopic pipe of the sampling mechanism is also known in the prior art and is not described in detail.
The inclined slide transfer device 6 comprises an inclined slide taking lifting motor fixed on a combined frame, wherein a first driving belt wheel is installed on a rotating shaft of the inclined slide taking lifting motor, a first shaft sleeve is fixed on the combined frame, a first vertical rotating shaft is installed in the first shaft sleeve, a first driven belt wheel is installed at the upper end of the first vertical rotating shaft, and the first driving belt wheel and the first driven belt wheel are in belt transmission; the lower end of the first vertical rotating shaft is in threaded connection with a first lifting sliding block, an inclined supporting plate is fixed at the lower end of the first vertical sliding block, an inclined pressing plate is hinged to the lower side of the inclined supporting plate through a pin shaft, and the inclined pressing plate is kept away from the inclined supporting plate under the pressure of a spring or a torsion spring; meanwhile, slideways are fixed on the combined frame in parallel; sliding shafts I are symmetrically arranged on two sides of the inclined pressing plate, and are arranged in corresponding slideways in a matching manner; the slideway comprises an upper section and a lower section which are in smooth transition, wherein the two sections are parallel to the combined rack, but the width d1 from the lower section to the combined rack is smaller than the width d2 from the upper section to the combined rack. Therefore, when the sliding shaft moves downwards, the sliding shaft is positioned at the lower section of the sliding way and is closer to the combined frame, so that the inclined pressing plate can be forced to be close to the inclined supporting plate, and the operation process of clamping the inclined slide is realized. When the first sliding shaft moves upwards, the first sliding shaft is located at the upper section of the sliding way and is far away from the combined frame, the inclined pressing plate can be released, the inclined pressing plate is far away from the inclined supporting plate, and the operation process of releasing the inclined glass slide is achieved.
For the purpose of cushioning and not damaging the flat glass slide 13, a flexible cushioning layer, or an elastic cushioning mechanism, may be provided in the area of the support surface below the flat glass slide 13. And a first flexible cushion block is arranged on the inner side of the L-shaped clamping mouth. And a second flexible cushion block is arranged on the inner side of the inclined pressing plate.
Claims (5)
1. The utility model provides a full-automatic microscope continuous detection system, contains microscope detection device and the control system of setting on the bottom plate, sample application of sample device and sample and suction nozzle box, characterized by: also comprises an inclined slide glass supply device, an inclined slide glass transfer device, a flat slide glass supply device and a slide glass transfer device;
the inclined slide transfer device and the sampling and sample adding device are fixed on the same combined frame and are driven to move transversely synchronously;
the inclined slide transfer device comprises a driving mechanism which can enable the lifting slide block to move vertically; the lower end of the first lifting sliding block is provided with an elastically opened clamping nozzle, a first sliding shaft is arranged on a nozzle piece capable of swinging of the clamping nozzle, and a guide sliding structure which enables the first sliding shaft to move downwards and then to be close to the combined frame so as to close the clamping nozzle is arranged on one side of the combined frame;
the inclined slide supply device comprises a shaft support and an inclined support which are fixed on the bottom plate, the upper end of the shaft support is hinged with an L-shaped swing rod through a rotating shaft, and one end of the L-shaped swing rod is in transmission connection with the lifting slide block; an inclined push plate capable of translating along the inclined support is installed on one side of the inclined support and is in transmission connection with the other end of the L-shaped swing rod; an inclined slide stacking box is fixed on the inclined support, the lower side of the inclined push plate is positioned at the bottom of the inclined slide stacking box, and when the inclined push plate translates upwards, an inclined slide at the bottom layer of the inclined slide stacking box can be pushed out upwards, so that the inclined slide at the bottom layer is pushed into the position of the clamping mouth;
the flat slide transfer device comprises a longitudinal movement mechanism and a transverse movement mechanism on the basis of the longitudinal movement mechanism, a support is fixed on the lower side of the front end of the transverse movement mechanism, an elastic closed clamping nozzle is installed on the support, a slide taking support pressing block is arranged on the bottom plate and below the flat slide transfer device, a slide unloading support pressing block is arranged at the position of the waste slide recovery box, and the clamping nozzle can be opened after being contacted with the slide taking support pressing block and the slide unloading support pressing block;
the flat glass slide supply device comprises a flat glass slide stacking box fixed on the base, the lower end of the flat glass slide stacking box is provided with an opening and is provided with a pushing plate capable of translating back and forth, and the pushing plate is connected with a transmission mechanism to realize the process of translating and pushing back and forth;
a pushing groove is formed in the upper side of the pushing plate, a flat glass slide in the flat glass slide stacking box can fall into the pushing groove, and the pushing groove pushes the flat glass slide to the clamping mouth position;
the flat glass slide transfer device moves along the longitudinal direction, takes out a flat glass slide from the flat glass slide supply device by using a clamping mouth, moves to the area of the microscope detection device in a transverse mode after sample adding and strickling operations, detects and records a sample on the flat glass slide by using the microscope detection device, and then returns to unload the flat glass slide and resets; the inclined slide transfer device and the sampling and sample adding device move synchronously, and the inclined slide transfer device and the sampling and sample adding device are matched with the inclined slide supply device and the slide supply device as well as the waste piece recovery box and the sample and suction nozzle box as follows:
(1) when the inclined slide transfer device and the sampling and sample adding device are simultaneously positioned at the upper sides of the inclined slide supply device and the flat slide supply device, the inclined slide transfer device moves downwards to a position B to take the inclined slide, and the sampling and sample adding device takes a sample
The device samples the flat glass slide;
(2) when the inclined slide transfer device and the sampling and sample adding device are simultaneously positioned above the flat slide supply device and the waste piece recovery box, the inclined slide transfer device continuously moves the inclined slide to the position C and presses one end of the flat glass slide, after the flat glass slide transfer device longitudinally pulls the flat glass slide, a sample on the flat glass slide is scraped, and at the moment, the sampling and sample adding device withdraws a suction nozzle after sample adding;
(3) when the inclined slide transfer device and the sampling and sample adding device are simultaneously positioned above the waste piece recovery box and the sample and suction nozzle box, the inclined slide transfer device is moved upwards to a position A to remove the inclined slide, and the sampling and sample adding device is inserted into the suction nozzle and absorbs the sample; the above (1) to (3) are circulated repeatedly.
2. The fully automatic microscope continuous inspection system of claim 1, wherein: the flat glass slide transfer device comprises two longitudinal slide ways which are fixed on the bottom plate in parallel, longitudinal slide blocks are respectively arranged on the two longitudinal slide ways, transverse slide ways are fixed on the two longitudinal slide blocks, a longitudinal driving mechanism which enables the longitudinal slide blocks to move on the longitudinal slide ways is arranged, transverse slide blocks are arranged on the transverse slide ways, and a transverse driving mechanism which enables the transverse slide blocks to move on the transverse slide ways is arranged; the front end lower side of the transverse sliding block is fixed with a support, an L-shaped clamping mouth is hinged to the support through a pin shaft, the upper side of the L-shaped clamping mouth extends out of the upper side of the transverse sliding block, and a spring or a torsion spring for keeping the clamping mouth and the transverse sliding block to be pressed tightly is arranged.
3. The fully automatic microscope continuous inspection system of claim 1, wherein: the inclined slide transfer device comprises an inclined slide taking lifting motor fixed on a combined frame, a first driving belt wheel is installed on a rotating shaft of the inclined slide taking lifting motor, a first shaft sleeve is fixed on the combined frame, a first vertical rotating shaft is installed in the first shaft sleeve, a first driven belt wheel is installed at the upper end of the first vertical rotating shaft, and the first driving belt wheel and the first driven belt wheel are in belt transmission; the lower end of the first vertical rotating shaft is in threaded connection with a first lifting sliding block, an inclined supporting plate is fixed at the lower end of the first lifting sliding block, an inclined pressing plate is hinged to the lower side of the inclined supporting plate through a pin shaft, and the inclined pressing plate is kept away from the inclined supporting plate under the pressure of a spring or a torsion spring; meanwhile, slideways are fixed on the combined frame in parallel; sliding shafts I are symmetrically arranged on two sides of the inclined pressing plate, and are arranged in corresponding slideways in a matching manner; the slideway comprises an upper section and a lower section which are in smooth transition, wherein the two sections are parallel to the combined rack, but the width d1 from the lower section to the combined rack is smaller than the width d2 from the upper section to the combined rack.
4. The fully automatic microscope continuous inspection system of claim 1, wherein: the sampling and sample adding device comprises a sampling lifting motor fixed on the combined frame, a driving belt wheel II is installed on a rotating shaft of the sampling lifting motor, a shaft sleeve II is fixed on the combined frame, a vertical rotating shaft II is installed in the shaft sleeve II, a driven belt wheel II is installed at the upper end of the vertical rotating shaft II, and the driving belt wheel II and the driven belt wheel II are in belt transmission; the lower end of the vertical rotating shaft II is in threaded connection with a second lifting slide block, and a sampling mechanism is fixed at the lower end of the second lifting slide block.
5. The fully automatic microscope continuous inspection system of claim 1, wherein: a flexible buffer layer is arranged in the pushing groove.
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| CN108760742B (en) * | 2018-08-08 | 2023-09-19 | 杭州上池科技有限公司 | Microscopic scanning imaging device and method for carrying out sample scanning imaging by using microscopic scanning imaging device |
| CN109612798B (en) * | 2018-12-25 | 2022-04-08 | 宁波察微生物科技有限公司 | Slide glass dyeing, mounting and scanning all-in-one machine |
| CN114354308B (en) * | 2021-12-27 | 2024-03-19 | 厦门大学 | Full-automatic film-making, dyeing and film-reading integrated machine |
| CN115220209B (en) * | 2022-04-19 | 2024-02-27 | 清华大学 | Operating device for assisting in acquiring microscopic image |
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