CN117706102B - Optical detection device and detection method for tumor rehabilitation - Google Patents
Optical detection device and detection method for tumor rehabilitation Download PDFInfo
- Publication number
- CN117706102B CN117706102B CN202410163824.4A CN202410163824A CN117706102B CN 117706102 B CN117706102 B CN 117706102B CN 202410163824 A CN202410163824 A CN 202410163824A CN 117706102 B CN117706102 B CN 117706102B
- Authority
- CN
- China
- Prior art keywords
- carrying
- tumor cell
- assembly
- groups
- groove
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 67
- 230000003287 optical effect Effects 0.000 title claims abstract description 28
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 15
- 210000004881 tumor cell Anatomy 0.000 claims abstract description 87
- 239000000463 material Substances 0.000 claims abstract description 47
- 230000005540 biological transmission Effects 0.000 claims abstract description 34
- 230000007246 mechanism Effects 0.000 claims description 56
- 230000004224 protection Effects 0.000 claims description 35
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 14
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 14
- 241001330002 Bambuseae Species 0.000 claims description 14
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 14
- 239000011425 bamboo Substances 0.000 claims description 14
- 239000011521 glass Substances 0.000 claims description 11
- 238000001179 sorption measurement Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 abstract description 2
- 230000001681 protective effect Effects 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000009979 protective mechanism Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the technical field of optical detection devices, and particularly relates to an optical detection device and a detection method for tumor rehabilitation, wherein the optical detection device comprises a detection assembly, a carrying assembly and a material conveying assembly; the material conveying component is movably sleeved between the detection component and the material carrying component, and is in transmission connection with the material carrying component; the light source is used for illuminating the tumor cell sample on the carrying component, the objective switches the objective groups after being rotationally adjusted through the objective switch, detection personnel are used for observing the tumor cell sample at the bottom of the objective after switching through the ocular to carry out detection analysis, and the carrying component is used for carrying the tumor cell sample on the carrying component for the second time, so that the loosening of the arrangement of the tumor cell sample is avoided, and the quality of optical detection is improved.
Description
Technical Field
The invention belongs to the technical field of optical detection devices, and particularly relates to an optical detection device and a detection method for tumor rehabilitation.
Background
At present, tumors can be subjected to pathological sampling in a puncturing mode, and then cell meats are amplified and observed through an optical microscope.
Through searching, in the prior art, china patent publication No. CN211603711U, the date of the grant notice: 2020-09-29 discloses a cell optical detection device based on biochip, including optical detection microscope body, optical detection microscope body's left side central point puts department fixed mounting has the objective table, and the surface of objective table inserts and is equipped with trapezoidal piece, and trapezoidal piece's fixed surface is connected with the observation platform, and the right side fixed mounting on the top of objective table has fixture, and is provided with the clamp plate on the fixture, and optical detection microscope body's bottom fixed mounting has the base. This application is provided with fixture, through placing the cell section at the surface of observing the platform, makes the clamp plate left side decline, conveniently fixes the cell section through the clamp plate to make things convenient for relevant staff to observe the research to it, through being provided with moving mechanism, through rotating the knob, make the overcoat rotate on the pivot surface, thereby promote articulated pole motion, make the universal wheel form the support to the device.
The device still has the following drawbacks: although the outer sleeve can rotate on the surface of the rotating shaft through rotating the knob, the hinging rod is pushed to move, so that the universal wheel pair device forms a support, the outer rod is pushed to move under the elastic force of the second spring every time, so that the right side of the pressing plate moves upwards, the left side of the pressing plate clamps the cell slices, the operation is inconvenient, the automation efficiency is low, in addition, a plurality of groups of tumor cell samples need to be manually transported in the detection process, the tumor cell samples are easy to be loosely arranged and easy to fall off.
Disclosure of Invention
The invention provides an optical detection device for tumor rehabilitation, which comprises a detection assembly, a carrying assembly and a material conveying assembly; the material conveying component is movably sleeved between the detection component and the material carrying component, and is in transmission connection with the material carrying component;
the detection assembly comprises a mirror arm, a first driving arm and a second driving arm; the lens arm is characterized in that a light source is embedded and installed at the bottom of the lens arm, an objective lens switcher is rotationally connected to the top end of the inner wall of the lens arm, a plurality of groups of objective lenses are arranged at the bottom of the objective lens switcher, the plurality of groups of objective lenses are arranged in an annular array with the central axis of the objective lens switcher as the center, a lens barrel is rotationally connected to the top of the lens arm, and two groups of ocular lenses are arranged on one side wall of the lens barrel.
Further, electric putter is installed in the embedding of a side wall of mirror arm, electric putter's output runs through the mirror arm and the transmission is connected with first electronic slip table, first actuating arm and second actuating arm fixed connection are at the outer wall of mirror arm, just first actuating arm and second actuating arm are the symmetry setting with electric putter's axis as the center, the tip of first actuating arm just is provided with first motor on one side of keeping away from the mirror arm, the output transmission of first motor is connected with first sample dish, just a plurality of first sample groove of group has been seted up to first sample dish, a plurality of groups first sample groove is the annular array setting with the axis of first sample dish as the center, the tip of second actuating arm just is provided with the second motor on one side of keeping away from the mirror arm, the output transmission of second motor is connected with the second sample dish, just a plurality of groups second sample dish has been seted up to the second sample dish, a plurality of groups the second sample groove uses the axis of second sample dish to be the center and is annular array setting.
Further, the carrier assembly comprises a storage mechanism and a protection mechanism; the storage mechanism is of a rectangular structure, one end of the storage mechanism is in transmission connection with the output end of the first electric sliding table, the protection mechanism is of an open annular structure, and the protection mechanism is movably sleeved outside the storage mechanism.
Further, the storage mechanism comprises a carrying plate; a linkage groove is formed at one end of the carrying plate, the linkage groove is movably clamped at the output end of the first electric sliding table, the carrying groove is formed at the top of the carrying plate, the bottom of the carrying plate is fixedly connected with a condenser, the condenser is vertically arranged at the bottom of the carrying groove, the outer wall of the carrying plate is fixedly connected with two groups of second electric sliding tables, the two groups of second electric sliding tables are symmetrically arranged by taking the central axis of the carrying plate as the center, the other end of the carrying plate is provided with an adjusting groove, and the inner wall of adjusting tank rotates and is connected with an adjusting cylinder, just the outer wall fixedly connected with printing opacity glass of adjusting cylinder, printing opacity glass's a lateral wall swing joint is in the edge of carrying the thing groove, carry the outer wall of thing board and be close to one side embedding of adjusting cylinder and install the third motor, the output of third motor is connected with the one end transmission of adjusting cylinder, carry the embedding on the thing board and install biax electric putter, just biax electric putter is located the one end of the electronic slip table of second.
Further, the protection mechanism comprises a protection cylinder; the utility model discloses a protection section of thick bamboo, protection section of thick bamboo's both ends are open structure, first open slot has been seted up at protection section of thick bamboo's top, just the second open slot has been seted up to protection section of thick bamboo's bottom, first open slot and second open slot are the symmetry setting with protection section of thick bamboo's axis as the center, first open slot sets up perpendicularly in objective switch's bottom, the second open slot sets up perpendicularly at the top of light source, two sets of hollow smooth chambeies have still been seted up to protection section of thick bamboo's outer wall, and two sets of hollow smooth chambeies are the symmetry setting with protection section of thick bamboo's axis as the center, two sets of hollow smooth chambeies all activity cup joint at the output of biax electric putter.
Further, the material conveying assembly comprises a conveying mechanism and two groups of driving mechanisms; both ends of the conveying mechanism are movably sleeved on the two groups of driving mechanisms, and the ends of the two groups of driving mechanisms are in transmission connection with the output end of the second electric sliding table.
Further, the conveying mechanism comprises two groups of conveying wheels; the center holes are formed in the center of the central axis of the conveying wheels, the conveying wheels are sleeved with conveying belts, the outer walls of the conveying belts are fixedly connected with a plurality of groups of micro vacuum pumps, adsorption holes are formed in the surfaces of the conveying belts and close to the positions of the micro vacuum pumps, and the adsorption holes are communicated with the air inlet ends of the micro vacuum pumps.
Further, the driving mechanism comprises a third driving arm; and one end of the third driving arm is provided with an assembly groove, and the assembly groove is in transmission connection with the output end of the second electric sliding table.
Further, the other end of the third driving arm is fixedly connected with a third electric sliding table, the output end of the third electric sliding table is in transmission connection with a fourth motor, the output end of the fourth motor is fixedly connected in the central hole, and the output end of the fourth motor is in transmission connection with the transmission wheel.
A detection method of an optical detection device for tumor rehabilitation comprises the following steps,
the material conveying assembly is used for sequentially conveying the tumor cell samples to the material carrying assembly, and automatically clamping the tumor cell samples after the tumor cell samples are contacted with the material carrying assembly;
illuminating the tumor cell sample on the cargo assembly by a light source;
switching a plurality of groups of objective lenses after rotating and adjusting through an objective lens switcher;
observing and detecting tumor cell samples at the bottom of the switched objective lens through an ocular lens by a detector;
and carrying out secondary material conveying on the tumor cell sample on the carrying component through the material conveying component.
The beneficial effects of the invention are as follows:
1. the tumor cell sample is sequentially transferred onto the carrying component through the material conveying component, and is automatically clamped after being contacted with the carrying component, the tumor cell sample on the carrying component is illuminated through the light source, a plurality of groups of objective lenses are switched after being rotationally regulated through the objective lens switcher, detection and analysis are carried out on the tumor cell sample at the bottom of the switched objective lenses through observation of detection personnel through the ocular lens, the tumor cell sample on the carrying component is secondarily conveyed again through the material conveying component, loosening of arrangement of the tumor cell sample is avoided, and the quality of optical detection is improved.
2. Through the cooperation of second electronic slip table, third electronic slip table and fourth motor, make the conveyer belt drive again and adjust, make the tumour cell sample after carrying thing inslot detection again by adsorb the back put into the second sample dish for retrieve the effect of tumour cell sample after the automated inspection, improved tumour cell sample optical detection's efficiency.
3. The tumor cell sample is stored through the carrier groove, and the third motor is utilized to drive the adjusting cylinder after the tumor cell sample is placed in the carrier groove, so that the light-transmitting glass is movably attached to the edge of the carrier groove and used for automatically compacting the tumor cell sample in the carrier groove, the tumor cell sample is in a stable state in the detection process, and the structural stability of the tumor cell sample in each detection process is improved.
4. After retracting through biax electric putter's output, make the protective cylinder to one side rotate ninety degrees back, utilize biax electric putter's output conflict to connect at the inner wall of protective cylinder again for the protective cylinder after the location rotation makes the protective cylinder carry out dustproof processing to carrying the top in thing groove, is used for this optical detection device to carrying each part in the thing subassembly when not using safety protection's effect.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention may be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 shows a schematic structural diagram of an optical detection device for tumor rehabilitation according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a detection assembly according to an embodiment of the present invention;
FIG. 3 is a schematic view of a carrier assembly according to an embodiment of the present invention;
FIG. 4 is a schematic diagram showing the structure of a storage mechanism according to an embodiment of the present invention;
FIG. 5 is a schematic view showing the structure of a protection mechanism according to an embodiment of the present invention;
FIG. 6 shows a schematic structural view of a material handling assembly according to an embodiment of the present invention;
FIG. 7 is a schematic view showing the structure of a conveying mechanism according to an embodiment of the present invention;
fig. 8 shows a schematic structural view of a driving mechanism according to an embodiment of the present invention.
In the figure: 1. a detection assembly; 11. a mirror arm; 12. an electric push rod; 13. a first electric slipway; 14. a first driving arm; 15. a first sample disk; 16. a second driving arm; 17. a second sample tray; 18. a light source; 19. an objective lens switcher; 110. an objective lens; 111. a lens barrel; 112. an eyepiece; 2. a carrier assembly; 21. a storage mechanism; 211. a carrying plate; 212. a linkage groove; 213. a carrying groove; 214. a condenser; 215. the second electric sliding table; 216. an adjustment tank; 217. an adjustment cylinder; 218. light-transmitting glass; 219. a third motor; 2110. double-shaft electric push rod; 22. a protective mechanism; 221. a protective cylinder; 222. a first open slot; 223. a second open slot; 224. a hollow sliding cavity; 3. a material conveying component; 31. a conveying mechanism; 311. a transfer wheel; 312. a central bore; 313. a conveyor belt; 314. a micro vacuum pump; 315. adsorption holes; 32. a driving mechanism; 321. a third drive arm; 322. an assembly groove; 323. a third electric slipway; 324. and a fourth motor.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The embodiment of the invention provides an optical detection device for tumor rehabilitation, which comprises a detection assembly 1, a carrying assembly 2 and a material conveying assembly 3; as illustrated by way of example in fig. 1.
The material carrying assembly 2 is in transmission connection with a side wall of the detection assembly 1, the joint of the detection assembly 1 and the material carrying assembly 2 is in right angle arrangement, the material conveying assembly 3 is movably sleeved between the detection assembly 1 and the material carrying assembly 2, and the material conveying assembly 3 is in transmission connection with the material carrying assembly 2.
Specifically, the transporting component 3 is used for sequentially transferring tumor cell samples onto the carrying component 2, and automatically clamps the tumor cell samples after the tumor cell samples are contacted with the carrying component 2, so that the risk that the tumor cells in pathological nuclear division phases are loosely arranged and easily fall off due to manual taking of the tumor cell samples is avoided, shaking is avoided when the tumor cell samples are optically detected, the detection accuracy is improved, after the optical detection is finished, the tumor cell samples on the carrying component 2 are transported for the second time again through the transporting component 3, and the manual taking process of the tumor cell samples after the optical detection is avoided.
The detection assembly 1 comprises a mirror arm 11, a first driving arm 14 and a second driving arm 16; as illustrated by way of example in fig. 2.
An electric push rod 12 is embedded and installed on one side wall of the mirror arm 11, the output end of the electric push rod 12 penetrates through the mirror arm 11 and is in transmission connection with a first electric sliding table 13, the first driving arm 14 and the second driving arm 16 are fixedly connected to the outer wall of the mirror arm 11, the first driving arm 14 and the second driving arm 16 are symmetrically arranged by taking the central axis of the electric push rod 12 as the center, a first motor is arranged at one side of the end part of the first driving arm 14 far away from the mirror arm 11, a first sample disc 15 is in transmission connection with the output end of the first motor, a plurality of groups of first sample grooves are formed in the first sample disc 15, the first sample grooves are arranged in an annular array by taking the central axis of the first sample disc 15 as the center, a second motor is arranged at one side of the end part of the second driving arm 16 far away from the mirror arm 11, a plurality of groups of second sample discs 17 are in transmission connection with the output end of the second motor, and the second sample discs 17 are arranged in an annular array by taking the central axis of the second sample discs 17 as the center;
the bottom of the mirror arm 11 is embedded and provided with a light source 18, the top end of the inner wall of the mirror arm 11 is rotationally connected with an objective lens switcher 19, the bottom of the objective lens switcher 19 is provided with a plurality of groups of objective lenses 110, the plurality of groups of objective lenses 110 are arranged in an annular array with the central axis of the objective lens switcher 19 as the center, the top of the mirror arm 11 is rotationally connected with a lens cone 111, and one side wall of the lens cone 111 is provided with two groups of ocular lenses 112.
Specifically, the output end of the electric push rod 12 pushes the first electric sliding table 13 to enable the carrying component 2 and the carrying component 3 to move synchronously, so that the tumor cell sample, the light source 18 and the objective lens 110 on the carrying component 2 are kept at the same vertical position after adjustment, and the tumor cell sample on the carrying component 2 is driven by the first electric sliding table 13 to enable the tumor cell sample to be adjusted at the interval position between the light source 18 and the objective lens 110, so that the tumor cell sample can be optically detected at different distances;
the first driving arm 14 drives the first sample disc 15 to rotate by using the first motor at the end part, so that the tumor cell sample to be detected on the first sample disc 15 rotates, the taking of the transporting component 3 is facilitated, the second driving arm 16 drives the second sample disc 17 to rotate by using the second motor at the end part, the sample grooves on the second sample disc 17 are switched, the sample grooves without the tumor cell sample are moved to the feeding end of the transporting component 3, and the feeding of the tumor cell sample after detection is facilitated.
The carrying assembly 2 comprises a storage mechanism 21 and a protection mechanism 22; as illustrated by way of example in fig. 3.
The storage mechanism 21 is of a rectangular structure, one end of the storage mechanism 21 is in transmission connection with the output end of the first electric sliding table 13, the protection mechanism 22 is of an open annular structure, and the protection mechanism 22 is movably sleeved outside the storage mechanism 21.
The storage mechanism 21 includes a carrier plate 211; as illustrated by way of example in fig. 4.
A linkage groove 212 is formed at one end of the carrying plate 211, the linkage groove 212 is movably clamped at the output end of the first electric sliding table 13, a carrying groove 213 is formed at the top of the carrying plate 211, a condenser 214 is fixedly connected to the bottom of the carrying plate 211, the condenser 214 is vertically arranged at the bottom of the carrying groove 213, two groups of second electric sliding tables 215 are fixedly connected to the outer wall of the carrying plate 211, the two groups of second electric sliding tables 215 are symmetrically arranged with the central axis of the carrying plate 211 as the center, an adjusting groove 216 is formed at the other end of the carrying plate 211, and the inner wall of the adjusting groove 216 is rotatably connected with an adjusting cylinder 217, and the outer wall of the adjusting cylinder 217 is fixedly connected with a transparent glass 218, a side wall of the transparent glass 218 is movably attached to and connected with the edge of the carrying groove 213, a third motor 219 is embedded and mounted on the outer wall of the carrying plate 211 and one side close to the adjusting cylinder 217, the output end of the third motor 219 is in transmission connection with one end of the adjusting cylinder 217, a double-shaft electric push rod 2110 is embedded and mounted on the carrying plate 211, and the double-shaft electric push rod 2110 is located at one end of the second electric sliding table 215.
The guard mechanism 22 includes a guard cylinder 221; as illustrated by way of example in fig. 5.
The both ends of protective cylinder 221 are open structure, first open slot 222 has been seted up at the top of protective cylinder 221, just second open slot 223 has been seted up to the bottom of protective cylinder 221, first open slot 222 and second open slot 223 are the symmetry setting with the axis of protective cylinder 221 as the center, first open slot 222 sets up the bottom at objective switch 19 perpendicularly, second open slot 223 sets up the top at light source 18 perpendicularly, two sets of hollow sliding chamber 224 have still been seted up to the outer wall of protective cylinder 221, and two sets of hollow sliding chamber 224 are the symmetry setting with the axis of protective cylinder 221 as the center, two sets of hollow sliding chamber 224 all movably cup joint the output at biax electric putter 2110.
Specifically, the carrying groove 213 is used for storing a tumor cell sample, and after the tumor cell sample is placed in the carrying groove 213, the third motor 219 is used for driving the adjusting cylinder 217, so that the transparent glass 218 is movably attached to the edge of the carrying groove 213, and is used for automatically pressing the tumor cell sample in the carrying groove 213, so that the tumor cell sample is in a stable state in the detection process;
the protection cylinder 221 uses the first opening slot 222 and the second opening slot 223 to enable the light source 18 to penetrate through the second opening slot 223 and extend into the condenser 214, irradiates the tumor cell sample stored in the carrying slot 213, uses the objective 110 to observe the tumor cell sample stored in the carrying slot 213 through the first opening slot 222, uses the hollow sliding cavity 224 to be slidingly connected with the output end of the dual-shaft electric push rod 2110, so that the protection cylinder 221 horizontally moves, the protection cylinder 221 is not touched during the material conveying operation, and after the output end of the dual-shaft electric push rod 2110 is retracted, the protection cylinder 221 is rotated ninety degrees to one side, and then the output end of the dual-shaft electric push rod 2110 is used for abutting and connecting with the inner wall of the protection cylinder 221 after positioning and rotation, so that the protection cylinder 221 performs dust-proof treatment on the top of the carrying slot 213.
The material conveying assembly 3 comprises a conveying mechanism 31 and two groups of driving mechanisms 32; as illustrated by way of example in fig. 6.
Both ends of the conveying mechanism 31 are movably sleeved on the two groups of driving mechanisms 32, and the ends of the two groups of driving mechanisms 32 are in transmission connection with the output end of the second electric sliding table 215.
The conveying mechanism 31 comprises two groups of conveying wheels 311; as illustrated by way of example in fig. 7.
The center holes 312 are formed in the center of the central axis of the two groups of conveying wheels 311, the conveying belts 313 are sleeved on the two groups of conveying wheels 311, the outer walls of the conveying belts 313 are fixedly connected with a plurality of groups of micro vacuum pumps 314, adsorption holes 315 are formed in the surfaces of the conveying belts 313 and close to the positions of the micro vacuum pumps 314, and the adsorption holes 315 are mutually communicated with the air inlet ends of the micro vacuum pumps 314.
The driving mechanism 32 includes a third driving arm 321; as illustrated by way of example in fig. 8.
The assembly groove 322 has been seted up to the one end of third actuating arm 321, just the assembly groove 322 is connected with the output transmission of second electronic slip table 215, the other end fixedly connected with third electronic slip table 323 of third actuating arm 321, just the output transmission of third electronic slip table 323 is connected with fourth motor 324, the output fixed connection of fourth motor 324 is in centre bore 312, just the output and the transfer wheel 311 transmission of fourth motor 324 are connected.
Specifically, the third electric sliding table 323 drives the conveyor belt 313, so that the adsorption hole 315 on the conveyor belt 313 is movably attached to the tumor cell sample on the first sample tray 15, the tumor cell sample is adsorbed on the conveyor belt 313 by using the continuous operation of the micro vacuum pump 314, the fourth motor 324 drives the conveying wheel 311 to rotate, the adsorbed tumor cell sample moves to one side of the carrying groove 213, the second electric sliding table 215 drives the third driving arm 321 to move, the adsorbed tumor cell sample moves to the position vertical to the upper side of the carrying groove 213, and the third electric sliding table 323 drives the conveyor belt 313 to move downwards again, so that the adsorbed tumor cell sample falls into the carrying groove 213;
the cooperation of the second electric sliding table 215, the third electric sliding table 323 and the fourth motor 324 makes the conveyor belt 313 drive and regulate again, so that the tumor cell sample detected in the carrying groove 213 is absorbed again and then put into the second sample tray 17 for automatically detecting and then recovering the tumor cell sample.
The optical detection device for tumor rehabilitation provided by the embodiment of the invention has the following working principle:
the third electric sliding table 323 drives the conveyor belt 313, so that the adsorption holes 315 on the conveyor belt 313 are movably attached to the tumor cell samples on the first sample tray 15, the tumor cell samples are adsorbed on the conveyor belt 313 by utilizing the continuous operation of the micro vacuum pump 314, the fourth motor 324 drives the conveying wheel 311 to rotate, the adsorbed tumor cell samples move to one side of the carrying groove 213, the second electric sliding table 215 drives the third driving arm 321 to move, the adsorbed tumor cell samples move to the position vertical to the upper side of the carrying groove 213, and the third electric sliding table 323 drives the conveyor belt 313 to move downwards again, so that the adsorbed tumor cell samples fall into the carrying groove 213;
through the cooperation of the second electric sliding table 215, the third electric sliding table 323 and the fourth motor 324, the conveyor belt 313 is driven and regulated again, so that the tumor cell sample detected in the carrying groove 213 is adsorbed again and then is put into the second sample tray 17 for automatic detection and then recovery of the tumor cell sample;
the carrier groove 213 is used for storing tumor cell samples, and after the tumor cell samples are placed in the carrier groove 213, the third motor 219 is used for driving the adjusting cylinder 217, so that the transparent glass 218 is movably attached to the edge of the carrier groove 213 and is used for automatically pressing the tumor cell samples in the carrier groove 213, and the tumor cell samples are in a stable state in the detection process;
the light source 18 can penetrate through the second open groove 223 and extend into the condenser 214 by utilizing the first open groove 222 and the second open groove 223 through the protective cylinder 221, the tumor cell sample stored in the carrying groove 213 is illuminated, the tumor cell sample stored in the carrying groove 213 is observed through the first open groove 222 by utilizing the objective lens 110, the protective cylinder 221 is horizontally moved by utilizing the hollow sliding cavity 224 to be connected with the output end of the double-shaft electric push rod 2110 in a sliding manner, the protective cylinder 221 is used for preventing the material conveying assembly 3 from touching the protective cylinder 221 during material conveying operation, and the output end of the double-shaft electric push rod 2110 is retracted, the protective cylinder 221 is rotated ninety degrees to one side and then is connected to the inner wall of the protective cylinder 221 by utilizing the output end of the double-shaft electric push rod 2110 in a abutting manner, so that the protective cylinder 221 is used for positioning and rotating the top of the carrying groove 213 is dustproof.
On the basis of the optical detection device for tumor rehabilitation, the embodiment of the invention also provides a detection method of the optical detection device for tumor rehabilitation, which comprises the following steps,
the material conveying assembly is used for sequentially conveying the tumor cell samples to the material carrying assembly, and automatically clamping the tumor cell samples after the tumor cell samples are contacted with the material carrying assembly;
illuminating the tumor cell sample on the cargo assembly by a light source;
switching a plurality of groups of objective lenses after rotating and adjusting through an objective lens switcher;
observing and detecting tumor cell samples at the bottom of the switched objective lens through an ocular lens by a detector;
and carrying out secondary material conveying on the tumor cell sample on the carrying component through the material conveying component.
Although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (3)
1. An optical detection device for tumor rehabilitation, which is characterized in that: comprises a detection component (1), a carrying component (2) and a material conveying component (3); the material carrying assembly (2) is in transmission connection with one side wall of the detection assembly (1), the connection part of the detection assembly (1) and the material carrying assembly (2) is arranged at a right angle, the material carrying assembly (3) is movably sleeved between the detection assembly (1) and the material carrying assembly (2), and the material carrying assembly (3) is in transmission connection with the material carrying assembly (2);
the detection assembly (1) comprises a mirror arm (11), a first driving arm (14) and a second driving arm (16); the lens system is characterized in that a light source (18) is embedded and installed at the bottom of the lens arm (11), an objective lens switcher (19) is rotationally connected to the top end of the inner wall of the lens arm (11), a plurality of groups of objective lenses (110) are arranged at the bottom of the objective lens switcher (19), the plurality of groups of objective lenses (110) are arranged in an annular array with the central axis of the objective lens switcher (19) as the center, a lens cone (111) is rotationally connected to the top of the lens arm (11), and two groups of ocular lenses (112) are arranged on one side wall of the lens cone (111);
the detection assembly (1) comprises a mirror arm (11), a first driving arm (14) and a second driving arm (16); an electric push rod (12) is embedded and installed on one side wall of the mirror arm (11), the output end of the electric push rod (12) penetrates through the mirror arm (11) and is in transmission connection with a first electric sliding table (13), the first driving arm (14) and the second driving arm (16) are fixedly connected to the outer wall of the mirror arm (11), the first driving arm (14) and the second driving arm (16) are symmetrically arranged by taking the central axis of the electric push rod (12) as the center, a first motor is arranged at one side of the end part of the first driving arm (14) far away from the mirror arm (11), a first sample disc (15) is in transmission connection with the output end of the first motor, a plurality of groups of first sample grooves are formed in the first sample disc (15), the first groups of first sample grooves are arranged in an annular array by taking the central axis of the first sample disc (15) as the center, a second motor is arranged at one side of the end part of the second driving arm (16) far away from the mirror arm (11), a second sample disc (17) is in transmission connection with the output end of the second motor, a plurality of second sample discs (17) are arranged in an annular array by taking the central axes of the second sample discs (17) as the second sample discs;
the carrying assembly (2) comprises a storage mechanism (21) and a protection mechanism (22); the storage mechanism (21) is of a rectangular structure, one end of the storage mechanism (21) is in transmission connection with the output end of the first electric sliding table (13), the protection mechanism (22) is of an open annular structure, and the protection mechanism (22) is movably sleeved outside the storage mechanism (21);
the storage mechanism (21) comprises a carrying plate (211); a linkage groove (212) is formed in one end of the carrying plate (211), the linkage groove (212) is movably clamped at the output end of the first electric sliding table (13), a carrying groove (213) is formed in the top of the carrying plate (211), a condenser (214) is fixedly connected to the bottom of the carrying plate (211), the condenser (214) is vertically arranged at the bottom of the carrying groove (213), two groups of second electric sliding tables (215) are fixedly connected to the outer wall of the carrying plate (211), the two groups of second electric sliding tables (215) are symmetrically arranged with the central axis of the carrying plate (211) as the center, an adjusting groove (216) is formed in the other end of the carrying plate (211), an adjusting cylinder (217) is rotatably connected to the inner wall of the adjusting groove (216), a light-transmitting glass (218) is fixedly connected to the outer wall of the adjusting cylinder (217), one side wall of the light-transmitting glass (218) is movably attached to the edge of the carrying groove (213), a third motor (219) is embedded and mounted on the outer wall of the carrying plate (211) and one side close to the adjusting cylinder (217), the output end of the third motor (219) is in transmission connection with one end of the adjusting cylinder (217), a double-shaft electric push rod (2110) is embedded and mounted on the carrying plate (211), the double-shaft electric push rod (2110) is positioned at one end of the second electric sliding table (215);
the material conveying assembly (3) comprises a conveying mechanism (31) and two groups of driving mechanisms (32); both ends of the conveying mechanism (31) are movably sleeved on the two groups of driving mechanisms (32), and the ends of the two groups of driving mechanisms (32) are in transmission connection with the output end of the second electric sliding table (215);
the conveying mechanism (31) comprises two groups of conveying wheels (311); the center of the central axis of each of the two groups of conveying wheels (311) is provided with a central hole (312), each of the two groups of conveying wheels (311) is sleeved with a conveying belt (313), the outer wall of each conveying belt (313) is fixedly connected with a plurality of groups of micro vacuum pumps (314), the surfaces of the conveying belts (313) and the positions close to the micro vacuum pumps (314) are provided with adsorption holes (315), and the adsorption holes (315) are communicated with the air inlet ends of the micro vacuum pumps (314);
the drive mechanism (32) comprises a third drive arm (321); an assembly groove (322) is formed in one end of the third driving arm (321), and the assembly groove (322) is in transmission connection with the output end of the second electric sliding table (215);
the other end of the third driving arm (321) is fixedly connected with a third electric sliding table (323), the output end of the third electric sliding table (323) is in transmission connection with a fourth motor (324), the output end of the fourth motor (324) is fixedly connected in the center hole (312), and the output end of the fourth motor (324) is in transmission connection with the conveying wheel (311).
2. The optical detection device for tumor rehabilitation according to claim 1, wherein: the protection mechanism (22) comprises a protection cylinder (221); the utility model discloses a dual-axis electric push rod (2110), including protection section of thick bamboo (221), protection section of thick bamboo (221) both ends are open structure, first open slot (222) have been seted up at the top of protection section of thick bamboo (221), just second open slot (223) have been seted up at the bottom of protection section of thick bamboo (221), first open slot (222) and second open slot (223) are the symmetry setting with the axis of protection section of thick bamboo (221) as the center, first open slot (222) set up perpendicularly in the bottom of objective switcher (19), second open slot (223) set up perpendicularly at the top of light source (18), two sets of hollow sliding chamber (224) have still been seted up to the outer wall of protection section of thick bamboo (221), and two sets of hollow sliding chamber (224) are the symmetry setting with the axis of protection section of thick bamboo (221) as the center, two sets of hollow sliding chamber (224) all activity cup joint the output at dual-axis electric push rod (2110).
3. A detection method of the optical detection device for tumor rehabilitation according to any one of claims 1 to 2, characterized in that: the detection method comprises the following steps:
the material conveying assembly is used for sequentially conveying the tumor cell samples to the material carrying assembly, and automatically clamping the tumor cell samples after the tumor cell samples are contacted with the material carrying assembly;
illuminating the tumor cell sample on the cargo assembly by a light source;
switching a plurality of groups of objective lenses after rotating and adjusting through an objective lens switcher;
observing and detecting tumor cell samples at the bottom of the switched objective lens through an ocular lens by a detector;
carrying out secondary material conveying on the tumor cell sample on the carrying component through the material conveying component;
the third electric sliding table drives the conveyor belt, so that the adsorption hole on the conveyor belt is movably attached to the tumor cell sample on the first sample tray, the tumor cell sample is adsorbed on the conveyor belt by utilizing the continuous operation of the micro vacuum pump, the fourth motor drives the conveyor wheel to rotate, the adsorbed tumor cell sample moves to one side of the carrying groove, the second electric sliding table drives the third driving arm to move, the adsorbed tumor cell sample moves to the position vertical to the upper side of the carrying groove, and the third electric sliding table drives the conveyor belt to move downwards again, so that the adsorbed tumor cell sample falls into the carrying groove;
the tumor cell sample is stored through the carrier groove, and the third motor is utilized to drive the adjusting cylinder after the tumor cell sample is placed in the carrier groove, so that the light-transmitting glass is movably attached to the edge of the carrier groove and used for automatically compressing the tumor cell sample in the carrier groove, and the tumor cell sample is in a stable state in the detection process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410163824.4A CN117706102B (en) | 2024-02-05 | 2024-02-05 | Optical detection device and detection method for tumor rehabilitation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410163824.4A CN117706102B (en) | 2024-02-05 | 2024-02-05 | Optical detection device and detection method for tumor rehabilitation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117706102A CN117706102A (en) | 2024-03-15 |
| CN117706102B true CN117706102B (en) | 2024-04-12 |
Family
ID=90162865
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410163824.4A Active CN117706102B (en) | 2024-02-05 | 2024-02-05 | Optical detection device and detection method for tumor rehabilitation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117706102B (en) |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4125828A (en) * | 1972-08-04 | 1978-11-14 | Med-El Inc. | Method and apparatus for automated classification and analysis of cells |
| EP0405480A2 (en) * | 1989-06-28 | 1991-01-02 | Kirin Beverage Corporation | Method of and apparatus for detecting microorganisms |
| WO2011041814A1 (en) * | 2009-10-07 | 2011-04-14 | Onkotec Gmbh | Analysis device and method |
| WO2011046807A2 (en) * | 2009-10-12 | 2011-04-21 | Ventana Medical Systems, Inc. | Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue |
| JP2013105737A (en) * | 2011-11-14 | 2013-05-30 | Laser-Spectra Kk | Microscopic laser mass spectrometer |
| JP2015227940A (en) * | 2014-05-30 | 2015-12-17 | 国立研究開発法人理化学研究所 | Optical microscope system and screening device |
| CN109342369A (en) * | 2018-10-26 | 2019-02-15 | 中国科学院苏州生物医学工程技术研究所 | The big visual field bio-imaging that is quickly detected for circulating tumor cell, scanning, analytical equipment |
| JP2019062768A (en) * | 2017-09-28 | 2019-04-25 | フロンティアバイオシステムズ株式会社 | Micro-section sampling system |
| CN110907412A (en) * | 2018-09-14 | 2020-03-24 | 益善生物技术股份有限公司 | Detection system and analysis detection method for circulating tumor cells |
| CN111896512A (en) * | 2020-08-10 | 2020-11-06 | 中南大学湘雅医院 | Lung cancer circulating tumor cell detection equipment |
| CN213987982U (en) * | 2020-11-04 | 2021-08-17 | 兰州交通大学 | Automatic rotatory intelligent leveling spectrometer |
| CN113370304A (en) * | 2021-05-26 | 2021-09-10 | 杭州电子科技大学 | Sawing-punching integrated preparation device and method for acellular bone regeneration repair material |
| CN216030806U (en) * | 2021-09-29 | 2022-03-15 | 济南格利特科技有限公司 | Throat swab robot for sampling oral fungi of patient |
| CN216309811U (en) * | 2021-11-23 | 2022-04-15 | 北京唯创博精生物科技有限公司 | Cell detection instrument for tumor detection |
| WO2022120603A1 (en) * | 2020-12-08 | 2022-06-16 | 宁波职业技术学院 | Microscope facilitating optical focusing |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8223428B2 (en) * | 2008-07-04 | 2012-07-17 | Olympus Corporation | Microscope |
-
2024
- 2024-02-05 CN CN202410163824.4A patent/CN117706102B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4125828A (en) * | 1972-08-04 | 1978-11-14 | Med-El Inc. | Method and apparatus for automated classification and analysis of cells |
| EP0405480A2 (en) * | 1989-06-28 | 1991-01-02 | Kirin Beverage Corporation | Method of and apparatus for detecting microorganisms |
| WO2011041814A1 (en) * | 2009-10-07 | 2011-04-14 | Onkotec Gmbh | Analysis device and method |
| WO2011046807A2 (en) * | 2009-10-12 | 2011-04-21 | Ventana Medical Systems, Inc. | Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue |
| JP2013105737A (en) * | 2011-11-14 | 2013-05-30 | Laser-Spectra Kk | Microscopic laser mass spectrometer |
| JP2015227940A (en) * | 2014-05-30 | 2015-12-17 | 国立研究開発法人理化学研究所 | Optical microscope system and screening device |
| JP2019062768A (en) * | 2017-09-28 | 2019-04-25 | フロンティアバイオシステムズ株式会社 | Micro-section sampling system |
| CN110907412A (en) * | 2018-09-14 | 2020-03-24 | 益善生物技术股份有限公司 | Detection system and analysis detection method for circulating tumor cells |
| CN109342369A (en) * | 2018-10-26 | 2019-02-15 | 中国科学院苏州生物医学工程技术研究所 | The big visual field bio-imaging that is quickly detected for circulating tumor cell, scanning, analytical equipment |
| CN111896512A (en) * | 2020-08-10 | 2020-11-06 | 中南大学湘雅医院 | Lung cancer circulating tumor cell detection equipment |
| CN213987982U (en) * | 2020-11-04 | 2021-08-17 | 兰州交通大学 | Automatic rotatory intelligent leveling spectrometer |
| WO2022120603A1 (en) * | 2020-12-08 | 2022-06-16 | 宁波职业技术学院 | Microscope facilitating optical focusing |
| CN113370304A (en) * | 2021-05-26 | 2021-09-10 | 杭州电子科技大学 | Sawing-punching integrated preparation device and method for acellular bone regeneration repair material |
| CN216030806U (en) * | 2021-09-29 | 2022-03-15 | 济南格利特科技有限公司 | Throat swab robot for sampling oral fungi of patient |
| CN216309811U (en) * | 2021-11-23 | 2022-04-15 | 北京唯创博精生物科技有限公司 | Cell detection instrument for tumor detection |
Non-Patent Citations (2)
| Title |
|---|
| 1 种气动物流传输系统传送血液制剂安全性评估;马怡然 等，;《中国输血杂志》;20140228;第27卷(第2期);第122-126页 * |
| Theoretical description of the transfer of vibrations from a sample to the cantilever of an atomic force microscope;S Hirsekorn et，;《Nanotechnology》;19971231;第8卷;第57-66页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117706102A (en) | 2024-03-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN207925105U (en) | A kind of flexible display screen detection device | |
| CN117706102B (en) | Optical detection device and detection method for tumor rehabilitation | |
| CN115980072A (en) | Multi-station five-degree-of-freedom automatic detection device | |
| CN113022995B (en) | Culture dish assembling production line | |
| CN214101471U (en) | Cell-phone center outward appearance check out test set | |
| CN212255102U (en) | Notebook shell outward appearance check out test set | |
| CN211504918U (en) | Clinical laboratory is with inspection sample making devices | |
| CN214895355U (en) | Platelet function analyzer | |
| CN116197767A (en) | Optical lens grinding device with adaptive clamping function and clamping method | |
| CN114324340B (en) | Medical blood alpha fetoprotein detection device | |
| CN217931354U (en) | TRIM automatic check out test set | |
| CN214373747U (en) | Smear device for clinical examination | |
| CN217600703U (en) | Public health preventive medicine collection fungus detection device | |
| CN110850702A (en) | Watch lens detector | |
| CN114486425A (en) | Continuous sheet pushing device of sheet pushing machine | |
| CN213933639U (en) | Small foreign matter detection device in soft infusion bag | |
| CN214408286U (en) | Slide glass tray conveyer and automatic mounting equipment | |
| CN210775522U (en) | Colloidal gold fluorescence integrated immunoassay instrument | |
| CN210015056U (en) | Device for detecting substrate | |
| CN112255236A (en) | Full-intelligent strip-shaped light source appearance detection equipment | |
| CN113305887A (en) | Forming device is used in processing of computer CD-ROM drive tray | |
| CN112557683A (en) | Piece workstation is read in system piece dyeing | |
| CN113551958B (en) | Linear motion sealing device | |
| CN218930984U (en) | Lens processing equipment | |
| CN221426413U (en) | Portable detector for rapid detection of synthetic diamond |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |