CN107753137B - Spinal optical fiber fixing support and fixation teaching method thereof - Google Patents

Abstract

The invention relates to a spinal optical fiber fixing bracket and a fixation teaching method thereof. The support main part is the rectangle, one of them 2 relative edges of a set of support main part respectively extend there are 2 fixed foot, and 2 of connecting on same edge the both ends on place limit are arranged respectively to fixed foot, and the support main part has the optic fibre jack, and support main part another opposite side extends to have strengthens fixed semicircle board. The optical fiber is convenient to implant and fix in spinal cord optogenetic research, and the relative relationship between the optical fiber and the surface of the spinal cord is more stable. The structure is simple and reasonable, the optical fiber transmission efficiency is high, the cost is low, and the fixation is firm and convenient.

Description

Spinal optical fiber fixing support and fixation teaching method thereof

Technical Field

The invention relates to the field of spinal optical fiber fixation, in particular to a spinal optical fiber fixing bracket and a fixation teaching method thereof.

Background

With the development of technologies such as optogenetics and the like in recent years, people have more deep understanding on the structure and function of a neural network of a brain. However, because of the anatomical features of the spinal cord, no optogenetic study has been made on the spinal cord level neural circuits of whole animals. The reason is that the spinal cord is under the encirclement of the bony structure of the spinal column, the range of motion of the spinal column of the animal is large, and the optical fiber is fixed with certain difficulty. So optogenetic studies of spinal cord level neural circuits to date have essentially employed ex vivo experiments on spinal cord sections.

Disclosure of Invention

The invention aims to provide a fixing bracket suitable for spinal optogenetic research and a fixing teaching method thereof.

The technical scheme for solving the technical problems is as follows: the utility model provides a vertebra optic fibre fixed bolster, including being flaky support main part and by a plurality of fixed feet that support main part week side outwards extended, it has the optical fiber hole to open in the support main part.

The invention has the beneficial effects that: the fixing feet are bent to be fixed on the spine, so that the optical fiber is firmly fixed, and the structure is simple and reasonable.

On the basis of the technical scheme, the invention can be further improved as follows.

Furthermore, the support main part is the rectangle, one of them 2 relative edges of a set of support main part respectively extend and have 2 fixed foot, and the 2 of same edge connection fixed foot branch places in the both ends on limit.

The beneficial effect of adopting the further scheme is that: the structure is simple and reasonable, and the fixation is firm.

Furthermore, the fixing feet are trapezoidal in shape, and the longer bottom edges of the fixing feet are connected with the support main body.

The beneficial effect of adopting the further scheme is that: simple and reasonable structure, small damage, and good practicability and universality.

Furthermore, another pair of edges of the bracket main body extend to form a semicircular plate. The other group of opposite edges refers to two edges without fixing feet.

The beneficial effect of adopting the further scheme is that: the semicircular plate extends the length of the fixing support, so that the fixing support is more stable to fix on the spine, and no movement exists between the vertebral bodies under the support.

Further, fixed foot with support main part is integrative to be set up, fixed foot with support main part junction is provided with the crease.

The beneficial effect of adopting the further scheme is that: when the fixing bracket is fixed on the spine, the fixing bracket is easier to bend to form a semi-encircling shape.

Further, the fixed support is made of a titanium plate with the thickness of 0.2 mm.

The beneficial effect of adopting the further scheme is that: small rejection reaction and more balanced fixing operation and fixing strength.

Further, a method for teaching spinal optogenetic research, comprising:

s1, measuring the size of a spine model;

s2, customizing the spinal optical fiber fixing support according to the specific size of the spinal model;

s3, placing the fixed support at a corresponding position of the vertebral model after sterilization, and bending the fixed support into a semi-encircling shape to fix the fixed support on the vertebral model;

s4, fixing the optical fiber in the optical fiber hole and enabling the optical fiber to be in corresponding contact with the surface of the spinal cord model.

The beneficial effect of adopting the further scheme is that: by adopting the method, a spinal cord optogenetic researcher learns how to fix the optical fiber on the spine.

Drawings

FIG. 1 is a schematic view of the fixing bracket according to the present invention;

FIG. 2 is a schematic diagram showing the length measurement of the fixing leg of the stand in the present invention;

FIG. 3 is a schematic view of the width measurement of the fixing leg of the stand in the present invention;

fig. 4 is a schematic view of measuring the length and width of the stent main body according to the present invention.

In the drawings, the components represented by the respective reference numerals are listed below:

1. a stent body; 2. a fixing leg; 3. an optical fiber hole; 4. a semicircular plate.

Detailed Description

The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.

As shown in fig. 1-4, a spinal optical fiber fixing bracket comprises a sheet-shaped bracket body 1 and a plurality of fixing legs 2 extending outwards from the periphery of the bracket body 1, wherein an optical fiber hole 3 is formed in the bracket body 1. Support main part 1 is the rectangle, 1 wherein 2 relative edges of a set of support main part respectively extend and have 2 fixed foot 2, and the 2 of same edge connection fixed foot 2 branch is placed in the both ends on limit. The fixing feet 2 are trapezoidal in shape, and the longer bottom edges of the fixing feet are connected with the support main body 1. And a semicircular plate 4 extends from the other pair of edges of the bracket main body 1. The other set of opposite sides refers to two sides without the fixing legs 2. The fixed foot 2 with support main part 1 an organic whole sets up, fixed foot 2 with support main part 1 junction is provided with the crease. The fixed support is made of a titanium plate with the thickness of 0.2 mm.

The specific scheme for the spinal cord optogenetic research is as follows:

after spinal cord injection and transfection for 3 weeks, animals were allowed to take prone position, isoflurane was inhaled for anesthesia, animal respiratory frequency and depth were closely observed, and isoflurane inhalation concentration was adjusted at any time. Skin preparation in operation area, disinfection with iodophor, and incision at the center of animal postnatal line with length of 4 cm. Incise layer by layer, and free towards the abdomen along with the subcutaneous superficial fascia layer.

On the side of spinal cord injection virus, exploring the most distal floating rib and determining a specific thoracic vertebra according to the floating rib, determining a target intervertebral space needing to be inserted with optical fibers upwards or downwards, and sharply breaking corresponding intervertebral collateral ligaments so as to enlarge the exposed intervertebral space; removing the corresponding spinous processes forming the target intervertebral space and the next segment of vertebral body and muscle tissues at two sides of the spinous processes, and using periosteum elevator to perform periosteum elevator so as to ensure that the vertebral arch bone is clearly exposed; and (3) using a scalpel under a 10-time operating microscope, cutting the ligament transversely along the bone margin of the far-end vertebral body in the target intervertebral space, cutting the ligament upwards, and using rongeur to bite a small amount of vertebral arch plates towards the near section and the far end respectively to expose the spinal cord of the virus injection side.

Respectively measuring the distance between the transverse process of the proximal vertebral body and the transverse process of the distal third section of the targeted intervertebral space, taking the distance as L1, the maximum value of the distance between the transverse processes of the vertebral body as W1, the vertical distance between the highest point of the transverse processes and the extension line of the vertebral pedicle as L2 and the width of the widest position of the transverse processes as W2; customizing a fixing bracket, wherein the length of the bracket main body 1 is L1, the width of the bracket main body 1 is W1, the height of the fixing foot 2 is L2, and the length of the bottom edge of the fixing foot 2 connected with the bracket main body 1 is W2;

placing the fixed support at a corresponding position, drilling an optical fiber hole by using an electric drill in an operation to ensure that the optical fiber insertion hole of the fixed support is opposite to a target section of spinal cord exposed below, and then fixing four transverse process fixing feet of the support below corresponding transverse processes in a transverse manner in pairs; measuring the distance between the optical fiber insertion space of the fixing frame and the surface of the target spinal cord segment, selecting optical fibers with corresponding lengths to be inserted into the dura mater, and slightly recessing the dura mater. And uniformly mixing dental cement, coating the dental cement on the joint of the optical fiber and the fixed support, and performing secondary reinforcement.

Compared with the prior art, the invention has the beneficial effects that: 1. the problems that the relative position of the optical fiber and the spinal cord cannot be effectively fixed and the spinal cord injury cannot be avoided in the whole animal spinal cord level optogenetics research are solved. 2. The optical fiber transmission efficiency is high. 3. The optical fiber is firmly fixed, the optical fiber fixing position is reliable, and no movement exists between the lower vertebral bodies of the fixing bracket. 4. The individual character is strong, and the spinal cord-optical fiber fixing device is suitable for spinal cord-optical fiber fixing of various experimental animals. 5. High operability and low cost.

The teaching method for spinal optogenetic research comprises the following steps:

s1, respectively measuring the distance between the transverse process of the proximal vertebral body and the transverse process of the distal third section of the vertebral body to form a target intervertebral space, taking the distance as L1, the maximum value of the transverse process distance of the vertebral body as W1, the vertical distance between the maximum point of the transverse process and the extension line of the vertebral pedicle as L2, and the width of the maximum width of the transverse process as W2

S2, customizing a fixed support, wherein the length of the support main body 1 is L1, the width of the support main body 1 is W1, the height of the fixed foot 2 is L2, and the length of the bottom edge of the fixed foot 2 connected with the support main body 1 is W2;

s3, placing the fixed support at a corresponding position of the vertebral model after sterilization, calibrating the position of the optical fiber hole, drilling the hole, and then bending the fixed support into a semi-encircling shape to fix the fixed support on the vertebral model;

s4, fixing the optical fiber in the optical fiber hole 3, mixing dental cement uniformly for the second time, coating the dental cement on the joint of the optical fiber and the fixed support, and reinforcing for the second time to finish fixing.

By adopting the method, a spinal cord optogenetic researcher learns how to fix the optical fiber on the spine.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (8)

1. The utility model provides a vertebra optic fibre fixed bolster, its characterized in that, including being flaky support main part (1) and by a plurality of fixed feet (2) of outside extension of support main part (1) week side, it has optical fiber hole (3) to open on support main part (1), optical fiber hole (3) are used for passing optic fibre, make optic fibre inserts to the dura mater.

2. The spinal optical fiber fixation bracket of claim 1, wherein: support main part (1) is the rectangle, support main part (1) wherein a set of relative 2 edges respectively extend and have 2 fixed foot (2), and the 2 of connecting on same edge fixed foot (2) are arranged in the both ends on place limit respectively.

3. The spinal optical fiber fixation bracket of claim 2, wherein: the fixing feet (2) are trapezoidal in shape, and the longer bottom edges of the fixing feet are connected with the support main body (1).

4. The spinal optical fiber fixation bracket of claim 3, wherein: and a semicircular plate (4) extends from the other pair of edges of the bracket main body.

5. The spinal optical fiber fixation bracket of claim 4, wherein: the fixing foot (2) and the support main body (1) are integrally arranged, and a crease is arranged at the joint of the fixing foot (2) and the support main body (1).

6. The spinal optical fiber fixation bracket of claim 5, wherein: the fixed support is made of a titanium plate with the thickness of 0.2 mm.

7. The spinal optical fiber fixation bracket of claim 6, wherein: and a semicircular plate (4) extends from the other pair of edges of the bracket main body (1).

8. A method for teaching spinal optogenetic studies to be applied to the spinal optical fiber fixation frame of claims 1-7, comprising:

s1, measuring the size of a spine model;

s2, customizing the spinal optical fiber fixing bracket according to the size of a spinal model, wherein the spinal optical fiber fixing bracket is defined in any one of claims 1 to 6;

s3, placing the fixed support at a corresponding position of the vertebral model after sterilization, and bending the fixed support into a semi-encircling shape to fix the fixed support on the vertebral model;

s4, fixing the optical fiber in the optical fiber hole (3).

Images