CN109248001A - A kind of construction method of the bone defect model of the bilateral fibula osteotomy production of machin - Google Patents
A kind of construction method of the bone defect model of the bilateral fibula osteotomy production of machin Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/30—Animals modified by surgical methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/106—Primate
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention relates to biomedical research technical field, in particular to the construction method of the bone defect model of a kind of bilateral fibula osteotomy production of machin.In the program the following steps are included: under aseptic condition, after anaesthetizing to animal, on operation limb, a small-sized thorn notch is done with scalpel in fibula end, is inserted into Kirschner wire from fibula end, is inserted into fibula ossis 2cm after fixed.A notch is then done on fibula, the muscle of blunt separation thereon makes fibula exposure, fibula surrounding soft tissue is retracted, mark the position for needing row osteotomy, the part that 1.5cm long is cut off on fibula, after the completion of osteotomy, inspection bone stump, the Kirschner wire for being placed in fibula end before this is continued into, across truncated position, the exposed Kirschner wire of distal fibula is cut off, the broken ends of fractured bone after cutting is hammered into fibula using formed punch and orthopedic mallet.The present invention provides and the more relevant experimental data of the mankind for the cause of disease for verifying bone defect generation and effective clinical treatment.
Description
Technical field
The present invention relates to biomedical research technical field, in particular to a kind of bilateral fibula osteotomy production of machin
The construction method of bone defect model.
Background technique
The structural intergrity of bone defect phalanges is destroyed, and is one of the problem of common clinical and orthopaedics therapy.Mesh
Before, the method for treating bone defect has autologous bone and allogenic bone transplantation, tissue engineering technique and gene therapy and growth factor, object
Manage the adjuvant treatment etc. of cure.Wound, infection, tumour, osteomyelitis operation wound clearing and various congenital disorders are to lead to bone
The main reason for defect.The drug effect observed by rodent and primate often has very very much not with the mankind
Together, the progress of medical technology is limited to a certain extent.
Therefore be badly in need of establishing a kind of bone defect model of bilateral fibula osteotomy production of machin, to verify bone defect generation
The cause of disease and effective clinical treatment provide and the more relevant experimental data of the mankind.
Summary of the invention
In view of problem above, the present invention provides a kind of bone defect models of the bilateral fibula osteotomy production of machin, are
The cause of disease and effective clinical treatment for verifying bone defect generation provide and the more relevant experimental data of the mankind.
In order to achieve the above-mentioned object of the invention, the present invention is the following technical schemes are provided: a kind of bilateral fibula osteotomy of machin
The construction method of the bone defect model of production, comprising the following steps:
A. Animal Anesthesia: intramuscular injection 0.05mg/kg atropine, after 5-10min, intramuscular injection 4mg/kg ketamine, into
Hereafter row anesthesia uses the 0.8-1.5L/min oxygen of the isoflurane containing 1.5-5% to do maintenance anesthesia；
B. art limb is fixed: experimental monkey groups are brought into operating room, set dorsal position, operation limb are fixed, then in opposite side limb uplink
Same operating procedure, after two foot of experimental monkey groups is wrapped up, using preparatory opening so that the operation underpad that foot stretches out covers it
Body；
C. surgical procedure: on operation limb, a small-sized thorn notch is done with scalpel in fibula end, with a 1.2mm
The Kirschner wire of diameter, 10cm long is inserted into from fibula end, after ensuring Kirschner wire fixation, is inserted into fibula ossis 2cm, with
The front notch of a 4-5cm long is done on fibula afterwards, the muscle of blunt separation thereon makes fibula exposure, uses hand-held traction
Device retracts fibula surrounding soft tissue, forms the opening of about 1cm wide, is marked on fibula using sterile pen and need row osteotomy
The position of art cut off the part of 1.5cm long with the concussion saw head of dental burr on fibula, after the completion of osteotomy, inspection bone stump,
Ensure to be covered at bone section without remaining periosteum, the Kirschner wire for being placed in fibula end before this is continued into, across truncated position,
It is inserted into opposite side broken ends of fractured bone 1cm depth, the exposed Kirschner wire of cutting distal fibula hammers into the broken ends of fractured bone after cutting using formed punch and orthopedic mallet
In fibula；
D. post surgery treatment: postoperative 3 days, experimental monkey groups will persistently receive no less than 0.02mg/kg buprenorphine intramuscular injection, often
It is spaced about 8 hours twice；It is at the end of operation and 3 days postoperative, using Ceftriaxone Sodium 50mg/kg intramuscular injection, to prevent from infecting；
Postoperative 3 days, the pain of at least 2 times monitoring animals, uncomfortable daily, and limbs use defect and other vital signs.
The step B needs to guarantee that all surgical procedures carry out under anesthesia and stringent aseptic condition, and animal receives
The same day of operation will be real by the hair cutting for acra of performing the operation, and using operative region around Iodophor and 70% ethyl alcohol cleaning-sterilizing
Monkey operation limb is tested using 360 ° of orientation disinfection preserved skins of Iodophor, operation limb proximal end and trunk spread aseptic operation drape, limb foot of performing the operation
It is wrapped up using sterile Elastic bandage.
The step C sprays Sterile Saline to fibula and its periphery using band needle asepsis injector and drops during excision
Temperature, to prevent the excessively high thermal damage for causing the broken ends of fractured bone of cut place temperature.
The step D, after animal after restoring in anesthesia, i.e., permissible experimental monkey groups are normally born a heavy burden and activity, food and water
It will be placed in cage bottom, to guarantee that animal can be easy to obtain, while the appetite postoperative for stimulation test monkey, fruit can be additionally provided
And vegetables.
After the completion of the modeling, miniature CT scan is selected, palpation, Biomechanics test and pathological section index etc. are to model
It is evaluated, proof modeling success, it is very high with clinical concordance.
The advantages of the present invention are the provision of a kind of bone defect of bilateral fibula osteotomy production of machin
Model provides and the more relevant experimental data of the mankind to verify the cause of disease and the effective clinical treatment of bone defect generation.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention without any creative labor, may be used also for those of ordinary skill in the art
To obtain other drawings based on these drawings.
Fig. 1 is weekly measured body weight data.
Fig. 2 is X-ray film.
Fig. 3 is pathological section.
Specific embodiment
With reference to the accompanying drawings and examples, further description of the specific embodiments of the present invention.Following embodiment is only
For clearly illustrating technical solution of the present invention, and not intended to limit the protection scope of the present invention.
1. experimental animal of embodiment
By experimental animal feeding in regular grade animal house, single cage raising, keeping room temperature is 29 DEG C of 20-, relatively wet
Spend 40-70%, fluorescent lamp circulation illumination in 12 hours, lighting hours 08:00-20:00, the free feeding of animal, free water, drink
It is to meet WHO drinking water specification through the purified tap water of water purifier with water.
Embodiment 2. is anaesthetized
Intramuscular injection 0.05mg/kg atropine first.After 5-10min, intramuscular injection 4mg/kg ketamine is anaesthetized.
Hereafter the 0.8-1.5L/min oxygen of the isoflurane containing 1.5-5% is used to do maintenance anesthesia.
3. surgical procedure of embodiment
The same day that animal undergos surgery by the hair cutting for acra of performing the operation, and uses Iodophor and 70% ethyl alcohol cleaning-sterilizing
Perinatal Therapy region.Subsequent experimental monkey groups are brought into operating room, set dorsal position.Operation limb surrounding is suspended and is fixed, uses 360 degree of Iodophor
Orientation sterilizes preserved skin.Operation limb proximal end and trunk spread aseptic operation drape.Operation limb foot is wrapped up using sterile Elastic bandage, with
Guarantee sterile always in surgical procedure.Then operation limb is fixed.Then in the same operating procedure of opposite side limb uplink.Work as experiment
After two foot of monkey is wrapped up, using preparatory opening so that the operation underpad that foot stretches out covers its body.
On operation limb, a small-sized thorn notch is done with scalpel in fibula end.With a 1.2mm diameter, 10cm long
Kirschner wire from fibula end be inserted into, after ensuring Kirschner wire fixation, be inserted into fibula ossis 2cm.Then on fibula
The front notch of a 4-5cm long is done, the muscle of blunt separation thereon makes fibula exposure.It is using hand-held retractor that fibula is all
Soft tissue traction is enclosed, the opening of about 1cm wide is formed.The position for needing row osteotomy is marked on fibula using sterile pen.With
The concussion saw head of dental burr cuts off the part of 1.5cm long on fibula.During excision, using band needle asepsis injector to calf
Sterile Saline cooling is sprayed on bone and its periphery, to prevent the excessively high thermal damage for causing the broken ends of fractured bone of cut place temperature.After the completion of osteotomy, inspection
Look into bone stump, it is ensured that be covered at bone section without remnants periosteum.The Kirschner wire for being placed in fibula end before this is continued into, it is horizontal
Across truncated position, it is deep to be inserted into opposite side broken ends of fractured bone 1cm.Cut off the exposed Kirschner wire of distal fibula, by the broken ends of fractured bone after cutting using formed punch and
Orthopedic mallet hammers into fibula.
4. postoperative care of embodiment
Postoperative 3 days, experimental monkey groups will persistently receive buprenorphine at least 0.02mg/kg intramuscular injection, and twice daily, interval is about
8 hours.It can continue to give if necessary.It is at the end of operation and 3 days postoperative, using Ceftriaxone Sodium 50mg/kg intramuscular injection, to prevent
Infection.Postoperative 3 days, the pain of at least 2 times monitoring animals, uncomfortable daily, and limbs use defect and other vital signs.According to
The recommendation of above situation and animal doctor may have change using the dosage of antalgesic and time.If the pain of animal has surmounted
The effective range of analgestic is stated, animal caregivers must contact animal doctor and study director to determine next step action.It is all dynamic
Object must be monitored closely until restoring from anesthesia.
After restoring in anesthesia, i.e., permissible experimental monkey groups are normally born a heavy burden and activity.Food and water will be placed in cage bottom, to protect
Card animal can be easy to obtain.Appetite postoperative for stimulation test monkey simultaneously, can be additionally provided fruits and vegetables.
5. weight of embodiment
A weight of animals is measured weekly.Measured body weight data are shown in Fig. 1 and table 1 weekly.Comparatively, animal is entire 16
Weight maintenance is stablized in the experimental period in week, only a small amount of fluctuation.
The weight weekly of table 1
Embodiment 6.X radiological survey X
The interval broken ends of fractured bone connecting line of X-ray examination art leg, callus formation and bone restored after surgery and every two weeks
Journey.X-ray film is as shown in Figure 2.
Embodiment 7. is drawn materials
Blood 3-5mL is taken before operation and anesthesia, to monitor health status.Serum is separated, is detected for subsequent antibody level.
In experimental endpoints, that is, after performing the operation 16 weeks, by animal euthanasia and take out the bone section repaired after osteotomy.After bone section exposure, make
Fibula is truncated above Kirschner wire with vibrating saw.With the gauze sponge impregnated through sterile saline by the bone section packet
It wraps up in, is placed in 50mL centrifuge tube, is stored in -20 DEG C.Torsion Biological Strength is carried out using MTS858 Material Testing Machine so as to subsequent
Learn detection.Before carrying out bone biomechanical detection, three-dimensional imaging and the volume bone mineral density of bone segment are generated using minitype CT.
Measurement data BVF (diaphysis fraction), BMC (bone mineral content) and the BMD (bone mineral density) of minitype CT are shown in Table 2.BMC
Show actual mineralized tissue content, it can be seen that operation significantly reduces the mineralized tissue at bone defect position.
2 minitype CT data of table
| Number of animals | Processing | BVF (%) | BMC(mg) | BMD(mg/cc) |
| On the left of 2776- | Operation | 0.2745 | 4.5122 | 447.6363 |
| On the left of 0712147- | It does not perform the operation | 0.6254 | 280.8371 | 680.1473 |
The result of Biomechanics test further demonstrates that minitype CT observes as a result, bone tissue after operation healing
Mechanical Data, including peak torque, rigidity and energy to failure have significant decrease compared to normal group.The dissection of 2776 left side fibula of animal
It can be twisted easily by hand when materials, therefore necessity that its sample is not tested.So related data is 0.
3 torsional test data of table
After Biomechanics test, sample is transferred in 10% neutral formalin and carries out tissue and fixes.Will be undecalcified, pass through
The sample of above-mentioned scanning makes slice, and the pathological evaluation of knitting is carried out using Trypan Blue and undyed slice.Disease
Reason slice proves modeling success, very high with clinical concordance at mould typical case.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (5)
1. a kind of construction method of the bone defect model of the bilateral fibula osteotomy production of machin, which is characterized in that including following
Step:
A. Animal Anesthesia: intramuscular injection 0.05mg/kg atropine, after 5-10min, intramuscular injection 4mg/kg ketamine carries out fiber crops
It is liquor-saturated, hereafter use the 0.8-1.5L/min oxygen of the isoflurane containing 1.5-5% to do maintenance anesthesia；
B. art limb is fixed: experimental monkey groups are brought into operating room, set dorsal position, operation limb are fixed, then in opposite side, limb uplink is same
Operating procedure, after the foot of experimental monkey groups two is wrapped up, using preparatory opening so that the operation underpad that foot stretches out covers its body；
C. surgical procedure: on operation limb, doing a small-sized thorn notch with scalpel in fibula end, with a 1.2mm diameter,
The Kirschner wire of 10cm long is inserted into from fibula end, after ensuring Kirschner wire fixation, is inserted into fibula ossis 2cm, is then existed
The front notch of a 4-5cm long is done on fibula, the muscle of blunt separation thereon makes fibula exposure, will using hand-held retractor
Fibula surrounding soft tissue retracts, and forms the opening of about 1cm wide, marks the portion for needing row osteotomy on fibula using sterile pen
Position cut off the part of 1.5cm long with the concussion saw head of dental burr on fibula, after the completion of osteotomy, inspection bone stump, it is ensured that do not have
There is remaining periosteum to be covered at bone section, the Kirschner wire for being placed in fibula end before this is continued into, across truncated position, insertion pair
Side broken ends of fractured bone 1cm is deep, and the broken ends of fractured bone after cutting is hammered into fibula using formed punch and orthopedic mallet by the exposed Kirschner wire of cutting distal fibula
It is interior.
D. post surgery treatment: postoperative 3 days, experimental monkey groups will persistently receive no less than 0.02mg/kg buprenorphine intramuscular injection, and daily two
It is secondary, it is spaced about 8 hours；It is at the end of operation and 3 days postoperative, using Ceftriaxone Sodium 50mg/kg intramuscular injection, to prevent from infecting；Postoperative 3
It, the pain of at least 2 times monitoring animals, uncomfortable daily, and limbs use defect and other vital signs.
2. the construction method of the bone defect model of the bilateral fibula osteotomy production of machin according to claim 1, special
Sign is that the step B needs to guarantee that all surgical procedures carry out under anesthesia and stringent aseptic condition, and animal receives
The same day of operation will be real by the hair cutting for acra of performing the operation, and using operative region around Iodophor and 70% ethyl alcohol cleaning-sterilizing
Monkey operation limb is tested using 360 ° of orientation disinfection preserved skins of Iodophor, operation limb proximal end and trunk spread aseptic operation drape, limb foot of performing the operation
It is wrapped up using sterile Elastic bandage.
3. the construction method of the bone defect model of the bilateral fibula osteotomy production of machin according to claim 1, special
Sign is that the step C sprays Sterile Saline to fibula and its periphery using band needle asepsis injector and drop during excision
Temperature, to prevent the excessively high thermal damage for causing the broken ends of fractured bone of cut place temperature.
4. the construction method of the bone defect model of the bilateral fibula osteotomy production of machin according to claim 1, special
Sign is that the step D, after animal after restoring in anesthesia, i.e., permissible experimental monkey groups are normally born a heavy burden and activity, and food and water will
Be placed in cage bottom, to guarantee that animal can be easy to obtain, while being the postoperative appetite of stimulation test monkey, can be additionally provided fruit and
Vegetables.
5. the building of the bone defect model of the bilateral fibula osteotomy production of machin described in any one of -4 according to claim 1
Method, it is characterised in that: after the completion of the modeling, select miniature CT scan, palpation, Biomechanics test and pathological section index
Deng evaluating model, proof modeling success is very high with clinical concordance.
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| CN115245141A (en) * | 2021-12-03 | 2022-10-28 | 上海市第六人民医院 | Animal model for observing bone change under mechanical factors and construction method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115245141A (en) * | 2021-12-03 | 2022-10-28 | 上海市第六人民医院 | Animal model for observing bone change under mechanical factors and construction method thereof |
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