CN107723239A - High flux roller bottle apparatus - Google Patents
High flux roller bottle apparatus Download PDFInfo
- Publication number
- CN107723239A CN107723239A CN201711182837.2A CN201711182837A CN107723239A CN 107723239 A CN107723239 A CN 107723239A CN 201711182837 A CN201711182837 A CN 201711182837A CN 107723239 A CN107723239 A CN 107723239A
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- CN
- China
- Prior art keywords
- sleeve member
- roller bottle
- high flux
- roller
- sprocket wheel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
- C12M27/12—Roller bottles; Roller tubes
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- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of high flux roller bottle apparatus, and it includes the sleeve member that at least one internal diameter is adapted with roller bottle external diameter, the drive component rotated for actuating sleeve barrier part.When carrying out cell culture, roller bottle is placed in the sleeve member being adapted to its external diameter, rotated using drive component actuating sleeve barrier part so as to drive roller bottle together to rotate, it is a kind of active drive mode, frictional force drives different from cellar culture device, not against roller and roller bottle appearance, low to the material and shape need of roller bottle, toxigenic capacity is cheap, and the sync rates that each roller bottle of mode of active drive rotates are high, so as to improve the horizontal homogeneity of cell growth.Sleeve member is arranged in 7 rows 7 and set, and each sleeve member interior edge length sleeve direction is provided with 4 roller bottles and houses position.Device processing sample size is big, can at most handle 196 roller bottles simultaneously, significant increase cell culture efficiency.
Description
Technical field
The invention belongs to biomedical devices technical field, is related to a kind of roller bottle apparatus, relates in particular to a kind of high pass
Measure roller bottle apparatus.
Background technology
During cell deep drainpipe, it is most important that a suitable environment is provided for cell growth, in body,
Cell usually exists with the laminated of densification, and cell tissue contains together particular components in the form of they are original and can be strong
The biofluid contact of the strong regulation biomolecule for influenceing cell function.And the cell for departing from living organism is typically difficult in adapt to pass
The environment of the microculture of system, causes that cell culture has slow growth rate, easy microbiological contamination, individual is big and has been depleted, is good
Oxygen but the characteristics of be unable to strong agitation, these features cause the Vessel Design for cultivating cell to complicate, traditional microbial reaction
Device can not typically be used directly to cultivate cell.
At present, biologic medical field generally use blake bottle and culture bag carry out cell culture, wherein blake bottle shape
Varied, bottleneck generally carries air-permeative filter film, makes cell not contaminated while gas permeability in bottle is ensured.Different is thin
Born of the same parents' training method is different, in order to fully improve culture efficiency and paste area, typically roller adhere-wall culture is used, by blake bottle
It is placed in the incubator with roller, blake bottle is positioned on the groove among roller, and roller utilizes roller and culture after rotating
Frictional force between bottle drives blake bottle to rotate, so that cell obtains moistening nutrition and divides increment, this training method ratio
Traditional square vase quiescent culture efficiency greatly improves.But roller bottle apparatus flux conventional on current market is smaller, is only capable of accommodating
With 8-16 blake bottle of processing, in addition, the driving force of blake bottle is mainly the frictional force between roller and blake bottle, this is to culture
The material of bottle requires higher, and processing cost is higher, and the sync rates that blake bottle rotates with roller are difficult to ensure that, cause cell to be trained
It is poor to support uniformity.
The content of the invention
Therefore, the technical problems to be solved by the invention are that traditional incubator processing sample size is small, efficiency is low, to culture
Bottle material requires high, blake bottle and roller poor synchronization, so as to propose a kind of culture efficiency it is high, can active drive, to blake bottle
The high flux roller bottle apparatus that material requires low, toxigenic capacity is cheap, automaticity is high.
In order to solve the above technical problems, the technical scheme is that：
The present invention provides a kind of high flux roller bottle apparatus, and it includes what at least one internal diameter was adapted with roller bottle external diameter
Sleeve member, the drive component for driving the sleeve member to rotate.
Preferably, the drive component include being connected with described sleeve member one end sprocket wheel, with the sprocket wheel be adapted to company
The chain and the motor for driving the chain movement and then being rotated with movable sprocket connect.
Preferably, also include being connected to the sleeve member both ends, the first panel for supporting the sleeve member
And second panel.
Preferably, one end that the sleeve member is connected with the sprocket wheel is connected to the first panel by bearing, even
The opposite end for being connected to sprocket wheel one end is connected to the second panel by cam guide.
Preferably, also include being used for the manipulator that the roller bottle is placed or taken out by sleeve member to the sleeve member.
Preferably, the sleeve member is formed by the steel wire weld axially and radially set along the roller bottle.
Set preferably, the sleeve member arranges in 7 rows 7, the position that the second panel corresponds to the sleeve member opens up
There is through hole；Sleeve member length direction described in each sleeve member interior edge is provided with 4 roller bottles and houses position.
Preferably, each sleeve member is connected to a sprocket wheel, one is connected to per row chain wheel and is adapted to the sprocket wheel
Chain.
Preferably, the robot end is additionally provided with the extension hook for taking out roller bottle on the inside of sleeve member.
Preferably, activity point of view of the roller bottle in the sleeve member is no more than ± 22.5 °.
The above-mentioned technical proposal of the present invention has advantages below compared with prior art：
(1) high flux roller bottle apparatus of the present invention, it includes at least one internal diameter and is adapted with roller bottle external diameter
Sleeve member, the drive component for driving the sleeve member to rotate.When carrying out cell culture, roller bottle is placed in and fitted with its external diameter
In the sleeve member matched somebody with somebody, rotated using drive component actuating sleeve barrier part so as to drive roller bottle together to rotate, be a kind of active drive side
Formula, frictional force drives different from cellar culture device, not against roller and roller bottle appearance, to the material and shape need of roller bottle
Low, toxigenic capacity is cheap, and the sync rates that each roller bottle of mode of active drive rotates are high, so as to improve cell growth level
Homogeneity.
(2) high flux roller bottle apparatus of the present invention, in addition to for being placed or by sleeve to the sleeve member
Part takes out the manipulator of the roller bottle, picks and places roller bottle using manipulator, improves the automaticity of device.
(3) high flux roller bottle apparatus of the present invention, the sleeve member along the roller bottle by axially and radially setting
The steel wire weld put forms.The steel wire axially and radially set along roller bottle can carry and limit movable model of the roller bottle in sleeve member
Enclose so that roller bottle can together rotate with sleeve member, and what steel wire surrounded accommodates the non-enclosed structure of sleeve member of roller bottle, saves
Material decreases the weight of sleeve member.
(4) high flux roller bottle apparatus of the present invention, the sleeve member are arranged in 7 rows 7 and set, the second panel
The position of the corresponding sleeve member offers through hole；Length sleeve direction described in each sleeve member interior edge is provided with 4 rollings
The accommodating position of bottle.So that the quantity of sleeve member can reach 49,4 roller bottles, device processing sample can be accommodated in each sleeve member
Amount is big, can at most handle 196 roller bottles simultaneously, significant increase cell culture efficiency.
(5) high flux roller bottle apparatus of the present invention, the robot end are additionally provided with for taking out sleeve
The extension hook of roller bottle on the inside of part.Position is housed due to being provided with 4 roller bottles inside each sleeve member along its length, in interior
The roller bottle in portion is not easy to be gripped by mechanical grip, therefore extends hook and serve the roller bottle that can go deep into sleeve member depths, further carries
The high mechanization degree of cell culture and pick and place sample efficiency.
Brief description of the drawings
In order that present disclosure is more likely to be clearly understood, specific embodiment and combination below according to the present invention
Accompanying drawing, the present invention is further detailed explanation, wherein
Fig. 1 is the structural representation of the high flux roller bottle apparatus described in the embodiment of the present invention；
Fig. 2 is the side view of the high flux roller bottle apparatus described in the embodiment of the present invention；
Fig. 3 is the structural representation for extending hook in the high flux roller bottle apparatus described in the embodiment of the present invention.
Reference is expressed as in figure：1- first panels；2- second panels；3- roller bottles；4- sleeve members；5- sprocket wheels；6- chains
Bar；7- cam guides；8- extends hook.
The present invention can be implemented with many different forms, should not be construed as limited to embodiment set forth herein, on the contrary,
There is provided these embodiments so that the disclosure is thorough and complete, and will fully convey the concept of the invention to art technology
Personnel, the present invention will be defined by the claims.In the accompanying drawings, for clarity, the size of each device and relative can be exaggerated
Size.Description of the invention is for distinguishing similar pair with the term " first " in claims and accompanying drawing, " second " etc.
As without for describing specific order or precedence.It should be appreciated that the data so used in the appropriate case can be with
Exchange.In addition, term " comprising ", " having " and their any deformation, it is intended that cover non-exclusive include.
Embodiment
Embodiment
The present embodiment provides a kind of high flux roller bottle apparatus, and it is arranged in incubator, for efficiently and equably
Cultivate cell, the high flux roller bottle apparatus as shown in Figure 1-2, including for supporting and installing the first face of other elements
Plate 1 (rear board) and second panel 2 (front panel), at least one internal diameter is provided between the first panel 1 and second panel 2
The sleeve member 4 being adapted with the external diameter of roller bottle 3, roller bottle 3 are used to cultivate cell, and the sleeve member 4 is connected with for driving its rotation
Drive component, the drive component actuating sleeve barrier part 4, which rotates and then driven, to be rotated with the roller bottle 3 that it is adapted in sleeve member 4, general
Traditional roller bottle is arranged at the top of roller, is driven roller bottle rotation to be changed to utilize indirectly using the frictional force between roller and roller bottle
The rotation of sleeve member 4 provides the power of an active drive for roller bottle 3 so that the roller bottle 3 in each drum member 4 is turned with identical
Speed rotates, and improves the homogeneity of cell culture.
Further, the drive component includes the sprocket wheel 5 being connected with described one end of sleeve member 4, and the sleeve member 4 connects
The one end for having sprocket wheel 5 is connected by bearing with first panel 1, and the adaptation of sprocket wheel 5 is connected with chain 6, in addition to for driving
The motor that chain 6 is moved and then rotated with movable sprocket 5.
The one end (opposite end for being connected with the one end of sprocket wheel 5) of the sleeve member 4 away from the sprocket wheel 5 passes through cam guide
7 are connected to the second panel 2.
In order to realize the automation mechanized operation of roller bottle culture, the high flux roller bottle apparatus is also included to the sleeve member
Placed in 4 or by the interior manipulator (not shown) for taking out the roller bottle of sleeve member 4.
To mitigate the weight of sleeve member 4, improving device flexibility and reducing installation cost, the sleeve member 4 is cylinder
Shape structure, it is formed by the steel wire weld of the radial and axial setting along cylinder (roller bottle 3), the non-envelope of the side wall of sleeve member 4 of formation
Close, play a part of supporting and limit the activity space of roller bottle 3 and roller bottle 3 can be driven to rotate, the roller bottle 3 is in sleeve member 4
Interior activity point of view is no more than ± 22.5 °.
In the present embodiment, the sleeve member 4 is 49, arranges and sets in 7 rows 7, the corresponding sleeve of the second panel 2
The position of part 4 offers through hole, is easy to place roller bottle 3 into sleeve member 4, and sleeve member 4 described in each interior edge of sleeve member 4 is grown
Degree direction (axial direction) is provided with 4 roller bottles and houses position, i.e., can house 4 roller bottles 3 in each sleeve member, so, the roller bottle training
Foster device can accommodate 7*7*4=196 roller bottle 3, suitable for Batch Culture cell, the significant increase efficiency of cell culture.
In the row sleeve member array of 7 row 7, each sleeve member 4 is respectively connected with a sprocket wheel 5, and each row's sleeve member 4 connects
Sprocket wheel 5 be commonly connected to the chain 6 being adapted to the sprocket wheel 5.
Because the inside of sleeve member 4 has 4 accommodating positions, the roller bottle 3 positioned at its depths can not be by the mechanical grip of manipulator
Gripping, therefore an extension hook 8 (as shown in Figure 3) is additionally provided with robot end, the extension hook 8 is used for sleeve member
The hook of roller bottle 3 of depths goes out in 4, further improves plant automation degree and treatment effeciency.
The course of work of high flux roller bottle apparatus described in the present embodiment is：First 3 bottles of roller bottle is clamped with mechanical arm
Lid, by vision positioning, roller bottle is put into sleeve member 4 according to suitable angle, is then shut off cultivating chamber door, opens driving electricity
Machine, motor drive chain 6 are moved, and are slowly rotated with the sprocket wheel 5 that it is adapted to so as to which chain 6 drives, the actuating sleeve barrier part of sprocket wheel 5
4 rotations finally drive the roller bottle 3 being arranged in sleeve member 4 slowly to rotate, and carry out cell culture, after culture terminates, manipulator folder
The bottle cap for holding outermost roller bottle 3 takes out outermost roller bottle 3, and internal roller bottle 3 uses machinery again after moving outer layer to using hook 8
Hand clamping is taken out.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
- A kind of 1. high flux roller bottle apparatus, it is characterised in that the set being adapted including at least one internal diameter with roller bottle external diameter Barrier part, the drive component for driving the sleeve member to rotate.
- 2. high flux roller bottle apparatus according to claim 1, it is characterised in that the drive component include with it is described The sprocket wheel of sleeve member one end connection, it is adapted to the sprocket wheel chain that connects and for driving the chain movement and then drive The motor that sprocket wheel rotates.
- 3. high flux roller bottle apparatus according to claim 2, it is characterised in that also include being connected to the set Barrier part both ends, first panel and second panel for supporting the sleeve member.
- 4. high flux roller bottle apparatus according to claim 3, it is characterised in that the sleeve member is connected with the chain One end of wheel is connected to the first panel by bearing, and the opposite end that the sleeve member is connected with sprocket wheel one end is led by cam The second panel is connected to device.
- 5. high flux roller bottle apparatus according to claim 4, it is characterised in that also include being used for the sleeve member Place or taken out by sleeve member the manipulator of the roller bottle.
- 6. high flux roller bottle apparatus according to claim 5, it is characterised in that the sleeve member is by along the roller bottle The steel wire weld axially and radially set forms.
- 7. high flux roller bottle apparatus according to claim 6, it is characterised in that the sleeve member is set in 7 rows 7 row Put, the position that the second panel corresponds to the sleeve member offers through hole；Sleeve member length described in each sleeve member interior edge Degree direction is provided with 4 roller bottles and houses position.
- 8. high flux roller bottle apparatus according to claim 7, it is characterised in that each sleeve member is connected to one Sprocket wheel, a chain being adapted to the sprocket wheel is connected to per row chain wheel.
- 9. high flux roller bottle apparatus according to claim 8, it is characterised in that the robot end is additionally provided with For taking out the extension hook of roller bottle on the inside of sleeve member.
- 10. high flux roller bottle apparatus according to claim 9, it is characterised in that the roller bottle is in the sleeve member Interior activity point of view is no more than ± 22.5 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711182837.2A CN107723239A (en) | 2017-11-23 | 2017-11-23 | High flux roller bottle apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711182837.2A CN107723239A (en) | 2017-11-23 | 2017-11-23 | High flux roller bottle apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107723239A true CN107723239A (en) | 2018-02-23 |
Family
ID=61218212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711182837.2A Pending CN107723239A (en) | 2017-11-23 | 2017-11-23 | High flux roller bottle apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107723239A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020086418A1 (en) * | 2000-10-24 | 2002-07-04 | Powell Alexander Robert | Cell culture apparatus |
| CN201367430Y (en) * | 2009-03-18 | 2009-12-23 | 苏州捷美电子有限公司 | Transmission mechanism of roller bottle cultivation device |
| CN202193792U (en) * | 2011-07-11 | 2012-04-18 | 黄山市中新生物科技有限公司 | Small-sized cell culture bottle roller |
| CN205999374U (en) * | 2016-08-31 | 2017-03-08 | 成都生物制品研究所有限责任公司 | Biological culture flask rotates bracket |
| CN207581836U (en) * | 2017-11-23 | 2018-07-06 | 深圳赛动生物自动化有限公司 | High-throughput roller bottle apparatus |
-
2017
- 2017-11-23 CN CN201711182837.2A patent/CN107723239A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020086418A1 (en) * | 2000-10-24 | 2002-07-04 | Powell Alexander Robert | Cell culture apparatus |
| CN201367430Y (en) * | 2009-03-18 | 2009-12-23 | 苏州捷美电子有限公司 | Transmission mechanism of roller bottle cultivation device |
| CN202193792U (en) * | 2011-07-11 | 2012-04-18 | 黄山市中新生物科技有限公司 | Small-sized cell culture bottle roller |
| CN205999374U (en) * | 2016-08-31 | 2017-03-08 | 成都生物制品研究所有限责任公司 | Biological culture flask rotates bracket |
| CN207581836U (en) * | 2017-11-23 | 2018-07-06 | 深圳赛动生物自动化有限公司 | High-throughput roller bottle apparatus |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 518000 Room 201, building A, No. 1, Qian Wan Road, Qianhai Shenzhen Hong Kong cooperation zone, Shenzhen, Guangdong (Shenzhen Qianhai business secretary Co., Ltd.) Applicant after: Shenzhen saai biological Automation Co., Ltd. Address before: 518000 Room 201, building A, No. 1, Qian Wan Road, Qianhai Shenzhen Hong Kong cooperation zone, Shenzhen, Guangdong (Shenzhen Qianhai business secretary Co., Ltd.) Applicant before: Shenzhen Beike biological race moving robot Co Ltd |