CN107714647A - A kind of oil-in-water type rubican nano-emulsion and preparation method thereof - Google Patents
A kind of oil-in-water type rubican nano-emulsion and preparation method thereof Download PDFInfo
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- CN107714647A CN107714647A CN201711139834.0A CN201711139834A CN107714647A CN 107714647 A CN107714647 A CN 107714647A CN 201711139834 A CN201711139834 A CN 201711139834A CN 107714647 A CN107714647 A CN 107714647A
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- emulsion
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 41
- 239000007908 nanoemulsion Substances 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title abstract description 18
- 239000004094 surface-active agent Substances 0.000 claims abstract description 38
- 239000004064 cosurfactant Substances 0.000 claims abstract description 31
- 239000000203 mixture Substances 0.000 claims abstract description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 11
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 11
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims description 8
- 239000003921 oil Substances 0.000 claims description 7
- -1 weigh rubican Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 2
- DPBVJRXPSXTHOL-UHFFFAOYSA-N propyl tetradecanoate Chemical compound CCCCCCCCCCCCCC(=O)OCCC DPBVJRXPSXTHOL-UHFFFAOYSA-N 0.000 claims description 2
- HIQIXEFWDLTDED-UHFFFAOYSA-N 4-hydroxy-1-piperidin-4-ylpyrrolidin-2-one Chemical compound O=C1CC(O)CN1C1CCNCC1 HIQIXEFWDLTDED-UHFFFAOYSA-N 0.000 abstract description 20
- 239000003814 drug Substances 0.000 abstract description 9
- 241000894006 Bacteria Species 0.000 abstract description 4
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 4
- 238000001727 in vivo Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 230000008685 targeting Effects 0.000 abstract description 3
- 230000004060 metabolic process Effects 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract 1
- 239000008280 blood Substances 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 7
- 101100248253 Arabidopsis thaliana RH40 gene Proteins 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 241000123069 Ocyurus chrysurus Species 0.000 description 4
- 230000000740 bleeding effect Effects 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 238000013112 stability test Methods 0.000 description 3
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 201000000736 Amenorrhea Diseases 0.000 description 2
- 206010001928 Amenorrhoea Diseases 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 2
- LVGKNOAMLMIIKO-UHFFFAOYSA-N Elaidinsaeure-aethylester Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC LVGKNOAMLMIIKO-UHFFFAOYSA-N 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- RGCKGOZRHPZPFP-UHFFFAOYSA-N alizarin Chemical compound C1=CC=C2C(=O)C3=C(O)C(O)=CC=C3C(=O)C2=C1 RGCKGOZRHPZPFP-UHFFFAOYSA-N 0.000 description 2
- 231100000540 amenorrhea Toxicity 0.000 description 2
- LVGKNOAMLMIIKO-QXMHVHEDSA-N ethyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC LVGKNOAMLMIIKO-QXMHVHEDSA-N 0.000 description 2
- 229940093471 ethyl oleate Drugs 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000023597 hemostasis Effects 0.000 description 2
- 238000000703 high-speed centrifugation Methods 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- YOBAEOGBNPPUQV-UHFFFAOYSA-N iron;trihydrate Chemical compound O.O.O.[Fe].[Fe] YOBAEOGBNPPUQV-UHFFFAOYSA-N 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 235000019515 salmon Nutrition 0.000 description 2
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000004443 Ricinus communis Nutrition 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- QGJOPFRUJISHPQ-NJFSPNSNSA-N carbon disulfide-14c Chemical compound S=[14C]=S QGJOPFRUJISHPQ-NJFSPNSNSA-N 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 239000005308 flint glass Substances 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002175 menstrual effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- WPWWKBNOXTZDQJ-UHFFFAOYSA-N xanthopurpurin Chemical compound C1=CC=C2C(=O)C3=CC(O)=CC(O)=C3C(=O)C2=C1 WPWWKBNOXTZDQJ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
- A61K9/1075—Microemulsions or submicron emulsions; Preconcentrates or solids thereof; Micelles, e.g. made of phospholipids or block copolymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/12—Ketones
- A61K31/122—Ketones having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/14—Esters of carboxylic acids, e.g. fatty acid monoglycerides, medium-chain triglycerides, parabens or PEG fatty acid esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/44—Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin
Abstract
The invention belongs to field of veterinary, specifically discloses a kind of oil-in-water type rubican nano-emulsion and preparation method thereof.It is made up of the composition of following weight/mass percentage compositions：Rubican 0.01~8.0%, surfactant 13~30%, cosurfactant 0~20%, oil 2.2~10.0%, surplus is water；Wherein, the surfactant is Tween 80, and the cosurfactant is absolute ethyl alcohol, and the oil is isopropyl myristate.Oil-in-water type rubican nano-emulsion of the present invention have the characteristics that viscosity is low, stability is good, it is dispersed it is strong, absorb rapid, targeting drug release, and the bioavilability of medicine can be improved, extend medicine half-life period in vivo, reduce toxic side effect；Oil-in-water type rubican nano-emulsion of the present invention also has the characteristics of surface tension is low, wellability is good, so medicine can be made to be attached to well on bacterium, penetrates into its metabolism of bacterium internal interference and plays antibacterial or bactericidal action.
Description
Technical field
The invention belongs to field of veterinary, and in particular to a kind of oil-in-water type rubican nano-emulsion and preparation method thereof.
Background technology
Rubican is a kind of material extracted from plant madder, and madder is a kind of time-honored vegetable colour, madder
It is cold in nature enter blood system, can cooling blood and hemostasis, and can stagnation resolvation.The blood trouble that goes out of all blood-heat bleeding can be selected.Cooling and activating blood, dissolving stasis, stimulate the menstrual flow.
For spitting blood, bleeding from five sense organs or subcutaneous tissue, metrostaxis, traumatism and bleeding, Amenorrhea stasis blocking, joint pain, tumbling and swelling.Cooling blood and hemostasis, promoting blood circulation：
This product is stopped blooding without staying the stasis of blood, for heat symptom-complex bleeding, Amenorrhea stomachache, traumatic injury.
The rubican extracted from madder（C14H8O4）It is a kind of salmon pink crystal or ochre yellow powder, property under normal temperature and pressure
Matter is stable, has excitant.Chinese is rubican, English name Alizarin.Alias name：1,2- dihydroxy -9,
10- amerantrones, 1,2- dihydroxy -1,2- dihydroxy castor quinone.Its physicochemical property, character salmon pink crystal or ochre yellow powder, it is close
Degree（g/mL,20/4℃）：1.06；Fusing point（℃）：289.5；Boiling point（DEG C, normal pressure）：430SU；Dissolubility：Be soluble in hot methanol and
25 DEG C of ether.Benzene, glacial acetic acid can be dissolved in, pyridine, carbon disulfide, be slightly soluble in water.
Growth of the rubican to staphylococcus aureus has inhibitory action, can suppress the penetrating of rat skin connective tissue
Property, it is similar to rutin, there is antiinflammatory action；It is cosmetic colors and lipstick pigment, without any secondary work and it can be used safely
With.But due to rubican in water it is insoluble, absorb not exclusively, bioavilability is low, Half-life in vivo is short, it is to heart in addition
There is certain toxicity.Therefore, a kind of suitable formulation how is found so as to improve the bioavilability of rubican, targeting, delay
The property released, extend its in vivo half-life period, heightening the effect of a treatment has become rubican and is urgently solved the problems, such as antibiotic substitute.
The content of the invention
The purpose of the present invention aims to provide a kind of oil-in-water type rubican nano-emulsion and preparation method thereof.
To achieve the above object, the technical scheme that the present invention takes is as follows：
A kind of oil-in-water type rubican nano-emulsion, is made up of the composition of following weight/mass percentage compositions：
Rubican 0.01~8.0%, surfactant 13~30%, cosurfactant 0~20%, oil 2.2~10.0%, surplus are
Water；Wherein, the surfactant is Tween-80, and the cosurfactant is absolute ethyl alcohol, and the oil is different for myristic acid
Propyl ester (IPM).
Preparation method, step are as follows：
（1）, form by weight/mass percentage composition, weigh rubican, surfactant, cosurfactant, oil, water, it is standby；
（2）, first rubican is completely dissolved in cosurfactant and surfactant, after stirring, add oil, stir
Mix；Finally it is added dropwise water, is stirred continuously while dropwise addition, until forming the system of homogeneous transparent, produces oil-in-water type rubican
Nano-emulsion.
The specifically used method and dosage of the rubican nano-emulsion of the present invention：Mixed drink, 65kg is watered with the mL of this product 100, is supplied
Livestock and poultry are freely drunk, and are used in conjunction 3 days.Points for attention：This product is only used for drinking water, not injectable, could be used after must being watered mixing, no
Can directly it use, laying cycle of laying hens disabling.
The present invention has advantages below：
1. oil-in-water type rubican nano-emulsion of the present invention has, viscosity is low, stability is good, it is dispersed it is strong, absorb rapid, targeting and release
The features such as medicine, and the bioavilability of medicine can be improved, extend medicine half-life period in vivo, reduce toxic side effect；
2. oil-in-water type rubican nano-emulsion of the present invention also has the characteristics of surface tension is low, wellability is good, so can make medicine
It is attached to well on bacterium, penetrates into its metabolism of bacterium internal interference and play antibacterial or bactericidal action.
Embodiment
Technical scheme is described in further detail below in conjunction with specific embodiment, but the protection model of the present invention
Enclose and be not limited thereto.
Embodiment 1
Prescription：Rubican 1.2g, surfactant 6.0g, cosurfactant 4.0g, oily 2.5g, water 13.6g；Wherein, it is described
Surfactant is Tween-80, and the cosurfactant is absolute ethyl alcohol, and the oil is isopropyl myristate (IPM).
Preparation method：
（1）, form by weight/mass percentage composition, weigh rubican, surfactant, oil, water, it is standby；
（2）, first rubican is completely dissolved in cosurfactant and surfactant, after stirring, add oil, stir
Mix；Finally it is added dropwise water, is stirred continuously while dropwise addition, until forming the system of homogeneous transparent, produces oil-in-water type rubican
Nano-emulsion.
Reference examples 1
Prescription：Rubican 0.01g, surfactant 13.38g, oily 2.43g, water 84.18g；Wherein, the surfactant is
APEO (40) rilanit special（RH40）, the oil is isopropyl myristate (IPM).
Preparation method：
（1）, form by weight/mass percentage composition, weigh rubican, surfactant, oil, water, it is standby；
（2）, first rubican is completely dissolved in surfactant, after stirring, add oil, stir and evenly mix；Finally it is added dropwise
Water, it is stirred continuously while dropwise addition, until forming the system of homogeneous transparent, produces oil-in-water type rubican nano-emulsion.
Reference examples 2
Prescription：Rubican 0.03g, surfactant 9.0g, oily 1.0g, water 20g；Wherein, the surfactant is polyoxy second
Alkene ether (40) rilanit special（RH40）, the oil is isopropyl myristate (IPM).
Preparation method is the same as reference examples 1.
Reference examples 3
Prescription：Rubican 0.3g, surfactant 9.0g, cosurfactant 4.0g, oily 1.0g, water 25.7g；Wherein, it is described
Surfactant is APEO (40) rilanit special（RH40）, the cosurfactant is 1,2-PD, described
Oil is isopropyl myristate (IPM).
Preparation method is the same as embodiment 1.
Reference examples 4
Prescription：Rubican 0.5g, surfactant 9.81g, cosurfactant 3.27g, oily 1.36g, water 21.47g；Wherein,
The surfactant is APEO (40) rilanit special（RH40）, the cosurfactant is 1,2-PD,
The oil is isopropyl myristate (IPM).
Preparation method is the same as embodiment 1.
Reference examples 5
Prescription：Rubican 0.9g, surfactant 15.0g, cosurfactant 5.0g, oily 2.2g, water 36.9g；Wherein, institute
It is Tween-80 to state surfactant, and the cosurfactant is absolute ethyl alcohol, and the oil is isopropyl myristate (IPM).
Preparation method is the same as embodiment 1.
Reference examples 6
Prescription：Rubican 0.9g, surfactant 15.0g, cosurfactant 5.0g, oily 5.0g, water 34.1g；Wherein, institute
It is Tween-80 to state surfactant, and the cosurfactant is absolute ethyl alcohol, and the oil is ethyl oleate.
Preparation method is the same as embodiment 1.
Reference examples 7
Prescription：Rubican 0.9g, surfactant 7.5g, cosurfactant 5.0g, oily 1.4g, water 18.5g；Wherein, it is described
Surfactant is Tween-80, and the cosurfactant is absolute ethyl alcohol, and the oil is isopropyl myristate (IPM).
Preparation method is the same as embodiment 1.
Reference examples 8
Prescription：Rubican 1.0g, surfactant 7.5g, cosurfactant 5.0g, oily 1.4g, water 15.0g；Wherein, it is described
Surfactant is Tween-80, and the cosurfactant is absolute ethyl alcohol, and the oil is isopropyl myristate (IPM).
Preparation method is the same as embodiment 1.
Reference examples 9
Prescription：Rubican 1.0g, surfactant 6.0g, cosurfactant 4.0g, oily 1.1g, water 15.0g；Wherein, it is described
Surfactant is Tween-80, and the cosurfactant is absolute ethyl alcohol, and the oil is ethyl oleate.
Preparation method is the same as embodiment 1.
Reference examples 10
Prescription：Rubican 1.2g, surfactant 7.5g, cosurfactant 5.0g, oily 1.4g, water 15.0g；Wherein, it is described
Surfactant is Tween-80, and the cosurfactant is absolute ethyl alcohol, and the oil is isopropyl myristate (IPM).
Preparation method is the same as embodiment 1.
Reference examples 11
Prescription：Rubican 1.3g, surfactant 6.0g, cosurfactant 4.0g, oily 1.1g, water 9.4g；Wherein, it is described
Surfactant is Tween-80, and the cosurfactant is absolute ethyl alcohol, and the oil is ethyl acetate.
Preparation method is the same as embodiment 1.
Stability test
1. high speed centrifugation
The rubican nano-emulsion prepared is taken in centrifuge tube, its property is observed again after centrifuging 20 min with 12000r/min rotating speed
Shape, it the results are shown in Table 1.
2. temperature stability test
The rubican nano-emulsion prepared is fitted into transparent flint glass bottle, seals, be positioned over 4 DEG C, room temperature（25℃）With
Keep sample under 60 DEG C of three kinds of temperature conditionss and investigate each 60d, sampled every 10d and observe its character, the results are shown in Table 2-4.
As seen from the above table：No matter high speed centrifugation or temperature stability test are passed through using the nano-emulsion of prescription of the present invention,
Clear is kept, has no the unstable demulsifying phenomenons such as layering, the precipitation of muddy or crystal, it is seen that be a kind of medicine of stabilization
Formulation.On the contrary, unstable demulsifying phenomenon is occurred using nano-emulsion obtained by the prescription of reference examples, solution is muddy or is layered, body
It is unstable.
Claims (2)
1. a kind of oil-in-water type rubican nano-emulsion, it is characterised in that be made up of the composition of following weight/mass percentage compositions：
Rubican 0.01~8.0%, surfactant 13~30%, cosurfactant 0~20%, oil 2.2~10.0%, surplus are
Water；Wherein, the surfactant is Tween-80, and the cosurfactant is absolute ethyl alcohol, and the oil is different for myristic acid
Propyl ester.
A kind of 2. method for preparing oil-in-water type rubican nano-emulsion as claimed in claim 1, it is characterised in that step is as follows：
（1）, form by weight/mass percentage composition, weigh rubican, surfactant, cosurfactant, oil, water, it is standby；
（2）, first rubican is completely dissolved in cosurfactant and surfactant, after stirring, add oil, stir
Mix；Finally it is added dropwise water, is stirred continuously while dropwise addition, until forming the system of homogeneous transparent, produces oil-in-water type rubican
Nano-emulsion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711139834.0A CN107714647B (en) | 2017-11-16 | 2017-11-16 | Oil-in-water type alizarin nanoemulsion and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711139834.0A CN107714647B (en) | 2017-11-16 | 2017-11-16 | Oil-in-water type alizarin nanoemulsion and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107714647A true CN107714647A (en) | 2018-02-23 |
| CN107714647B CN107714647B (en) | 2020-04-10 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302449A (en) * | 2011-08-25 | 2012-01-04 | 西北农林科技大学 | Oil-in-water cinnamic aldehyde nano emulsion medicament |
| CN104027408A (en) * | 2014-05-14 | 2014-09-10 | 河南牧翔动物药业有限公司 | Oil-in-water type compound apramycin nano-emulsion |
| CN104352566A (en) * | 2014-10-21 | 2015-02-18 | 河南牧翔动物药业有限公司 | Oil-in-water compound chlortetracycline nanoemulsion |
| CN104642312A (en) * | 2015-02-13 | 2015-05-27 | 兰州大学 | Rheum officinale free anthraquinone composition for preventing plant powdery mildew and nano emulsion preparation |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302449A (en) * | 2011-08-25 | 2012-01-04 | 西北农林科技大学 | Oil-in-water cinnamic aldehyde nano emulsion medicament |
| CN104027408A (en) * | 2014-05-14 | 2014-09-10 | 河南牧翔动物药业有限公司 | Oil-in-water type compound apramycin nano-emulsion |
| CN104352566A (en) * | 2014-10-21 | 2015-02-18 | 河南牧翔动物药业有限公司 | Oil-in-water compound chlortetracycline nanoemulsion |
| CN104642312A (en) * | 2015-02-13 | 2015-05-27 | 兰州大学 | Rheum officinale free anthraquinone composition for preventing plant powdery mildew and nano emulsion preparation |
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| CN107714647B (en) | 2020-04-10 |
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Denomination of invention: The invention relates to an oil in water alizarin nano emulsion and a preparation method thereof Effective date of registration: 20211224 Granted publication date: 20200410 Pledgee: China Construction Bank Corporation Zhengzhou Railway Sub Branch Pledgor: HENAN SOAR VETERINARY PHARMACEUTICAL Co.,Ltd.|HENAN MUXIANG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021980016170 |
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Granted publication date: 20200410 Pledgee: China Construction Bank Corporation Zhengzhou Railway Sub Branch Pledgor: HENAN SOAR VETERINARY PHARMACEUTICAL Co.,Ltd.|HENAN MUXIANG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021980016170 |
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Denomination of invention: A water in oil type Alizarin nanoemulsion and its preparation method Granted publication date: 20200410 Pledgee: China Construction Bank Corporation Zhengzhou Railway Sub Branch Pledgor: HENAN SOAR VETERINARY PHARMACEUTICAL Co.,Ltd.|HENAN MUXIANG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024980004821 |
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