CN107699515A - A kind of compound probiotic and preparation method thereof, application - Google Patents
A kind of compound probiotic and preparation method thereof, application Download PDFInfo
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- CN107699515A CN107699515A CN201710874858.4A CN201710874858A CN107699515A CN 107699515 A CN107699515 A CN 107699515A CN 201710874858 A CN201710874858 A CN 201710874858A CN 107699515 A CN107699515 A CN 107699515A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Abstract
The present invention relates to livestock and poultry cultivation, the depollution of environment and breeding waste processing technology field, specially a kind of compound probiotic and preparation method thereof, application.Technical scheme is as follows：Compound probiotic is mainly formulated by following strain is compound：Lactobacillus delbrueckii, lactobacillus bulgaricus, Lactobacillus helveticus, lactobacillus acidophilus, brewer's yeast, wine yeast, distillery yeast, fodder yeast, oil yeast, upper strata yeast, torula nigra, film-forming yeast, photosynthetic bacteria, bacterial digestion, bayesian sulfur bacteria, Nocard's bacillus, Sphaerotilus natans.Compound probiotic provided by the invention can be in rapid degrading cultivation on-site air Common detrimental gas and remove stink, and the feedstuff-meat ratio and the incidence of disease of livestock and poultry can be significantly reduced, in addition, compound probiotic cost provided by the invention is cheap, it is easy to use, it is widely used.
Description
Technical field
The present invention relates to a kind of compound probiotic and preparation method thereof, application, belong to livestock and poultry cultivation, the depollution of environment and cultivation
Waste Disposal Technology field.
Background technology
Stock raising waste is handled currently with beneficial microbe, such as excrement, sewage, most of is to use anaerobic fermentation
Processing scheme biogas or oxygen consumption fermentation process scheme, such as paper《The research of the Northeast's livestock and poultry cultivation night soil treatment technology》Breathe out
That shore Vocationl Technical College journal, 2013 (2)：119-120；With《The research and application of livestock breeding wastewater treatment technology》Agricultural
ACTA Scientiae Circumstantiae, 2006,25 (s1)：374-377,《Probiotics and its reasonable employment in aquaculture》Jiangxi animal and veterinary is miscellaneous
Will, 2009 (01)：26-29, the middle every technology used or complex art, its existing problems and disadvantages are as follows：
(1) sewage auxiliary facility input is high, and power consumption is high, and cost is high, and north of china in winter is incomplete in fermentation, treatment effect
Difference.
(2) fermentation process of excrement is fermented using compost fermentation or closing anaerobic, transport secondary pollution be present.
(3) excrement pot type fermentation or compost treatment are cultivated, and all unavoidable release of sewage disposal process is harmful to
Odour, pollute atmospheric environment.
(4) existing processing equipment and method can not purify the air in poultry house and the air of cultivation place.
(5) cultivation on-site can not form probiotics biological immune environment.
(6) alimentary canal beneficial microbe environment is poor, and feed conversion rate is low, and disease of digestive tract easily occurs, in order to prevent disease
Disease, using substantial amounts of antibiotic, bring the food safety hazards of animal.
The content of the invention
In view of the shortcomings of the prior art, the present invention provides a kind of compound probiotic and its preparation method and application, have into
The characteristics of this is low, application process is succinct, efficient.
Technical scheme is as follows：A kind of compound probiotic, it is formulated by following strain is compound：
Lactobacillus delbrueckii (L.delbrueckii) (80-120) × 106CFU/ milliliters；
Lactobacillus bulgaricus (L.bulgaricus) (130-170) × 106CFU/ milliliters；
Lactobacillus helveticus (L.helviticus) (80-120) × 106CFU/ milliliters；
Lactobacillus acidophilus (L.acidophilus) (180-220) × 106CFU/ milliliters；
Brewer's yeast (brewer ' syeast) (180-220) × 106CFU/ milliliters；
Wine yeast (wineyeast) (80-120) × 106CFU/ milliliters；
Distillery yeast (alcoholyeast) (80-120) × 106CFU/ milliliters；
Fodder yeast (fodderyeast) (180-220) × 106CFU/ milliliters；
Oil yeast (petroleumyeast) (30-70) × 106CFU/ milliliters；
Upper strata yeast (topyeast) (30-70) × 106CFU/ milliliters；
Torula nigra (blackyeast) (30-70) × 106CFU/ milliliters；
Film-forming yeast (filmyeast) (30-70) × 106CFU/ milliliters；
Photosynthetic bacteria (photosyntheticbacteria) (80-120) × 106CFU/ milliliters；
Bacterial digestion (Nitrifyingbacteria) (130-170) × 106CFU/ milliliters；
Bayesian sulfur bacteria (Beggiatoa) (80-120) × 106CFU/ milliliters；
Nocard's bacillus (Nocardia) (80-120) × 106CFU/ milliliters；
Sphaerotilus natans (Sphaerotilusnatans) (30-70) × 106CFU/ milliliters；
The optimum formula of the compound probiotic is as follows：
Lactobacillus delbrueckii 100 × 106CFU/ milliliters；
Lactobacillus bulgaricus 150 × 106CFU/ milliliters；
Lactobacillus helveticus 100 × 106CFU/ milliliters；
Lactobacillus acidophilus 200 × 106CFU/ milliliters；
Brewer's yeast 200 × 106CFU/ milliliters；
Wine yeast 100 × 106CFU/ milliliters；
Distillery yeast 100 × 106CFU/ milliliters；
Fodder yeast 200 × 106CFU/ milliliters；
Oil yeast 50 × 106CFU/ milliliters；
Upper strata yeast 50 × 106CFU/ milliliters；
Torula nigra 50 × 106CFU/ milliliters；
Film-forming yeast 50 × 106CFU/ milliliters；
Photosynthetic bacteria 100 × 106CFU/ milliliters；
Bacterial digestion 150 × 106CFU/ milliliters；
Bayesian sulfur bacteria 100 × 106CFU/ milliliters；
Nocard's bacillus 100 × 106CFU/ milliliters；
Sphaerotilus natans 50 × 106CFU/ milliliters.
The preparation method of the compound probiotic is as follows：
(1) liquid medium is prepared：50 parts of brown sugar, 50 parts of white sugar, 20 parts of glucose, 0.5 part of vitamin C, skimmed milk power 10
Part, 1000 parts of distilled water, it is mixed to form liquid medium；
(2) composite bacteria is prepared：It is compound in proportion after each individual plant strain is activated respectively, obtain composite bacteria；
(3) original bacteria liquid is prepared：10 parts of composite bacteria prepared by step (2) is put into the training of 1000 parts of steps (1) preparations
Support in base fluid, in 35 DEG C of -37 DEG C of sealed fermentings 3-5 days, opening vessel port and smelling had sour-sweet taste activate successfully, closed preservation work
Used for original bacteria liquid, the pH of the original bacteria liquid is 4.0-5.0；
(4) second order fermentation nutrient solution is prepared：Brown sugar and white sugar mixture 9.6wt%, vitamin C 0.2wt%, skimmed milk power
0.1wt%, urea 0.1wt%, remaining is except chlorine water, is mixed to form second order fermentation nutrient solution；Brown sugar and the white sugar mixture
The mass ratio of middle brown sugar and white sugar is 1:1；
(5) expansion is numerous obtains compound probiotic：Original bacteria liquid is added to the second order fermentation culture prepared in second order fermentation tank
In liquid, in 35 DEG C of -37 DEG C of sealed fermentings 3-5 days, opening vessel port and smelling had sour-sweet taste to expand numerous success, obtains compound prebiotic
Bacterium；The pH of the compound probiotic is 3.0-4.0, total number of bacteria >=5,000,000,000/milliliter.
The present invention is claimed described compound probiotic and is reducing the livestock and poultry incidence of disease, reduction livestock and poultry feedstuff-meat ratio, carrying simultaneously
High feed conversion rate, poultry excrement and urine disposal, livestock breeding wastewater, improvement its feeding environment, prevention disease of alimentary tract of livestock husbandry and birds side
The application in face.
Application method is：(1) when reducing the livestock and poultry incidence of disease, reduction livestock and poultry feedstuff-meat ratio, improving food conversion ratio application, to
In feed compound probiotic is added in 3wt% ratios；(2) in terms of its feeding environment, prevention disease of alimentary tract of livestock husbandry and birds is improved
Use vaporific spray using breeding environment, the adding proportion of compound probiotic is 0.1wt%；(3) in poultry excrement and urine disposal, poultry
During fowl cultivating wastewater purification, uniformly mixed in the addition of 0.1wt% ratios.
Beneficial effects of the present invention are as follows：
(1) compound probiotic provided by the invention can be in rapid degrading cultivation on-site air Common detrimental gas simultaneously removes
Stink, such as hydrogen sulfide, ammonia, volatile fatty acid, trimethylamine, methane, formaldehyde, scatol, thio-alcohol pernicious gas are removed, is sprayed
It can remove after spilling, cultivation on-site harmful substance is reduced by 90%, after being used continuously 3 weeks, you can accomplish to cultivate place Wu Te
There are animal scent and foul smell.There is the purification function of good environment and air.
(2) compound probiotic cost provided by the invention is cheap, easy to use, is widely used.
(3) compound probiotic provided by the invention carries out inoculation processing in the cultivation on-site can of livestock and poultry to excrement,
Reach excrement cleaning without foul smell, secondary conveying is pollution-free, and either compost or Concentrated fermentation are carried out all in odorless；In addition
The organic fertilizer that excrement can also be applied to converts, and without heating process.
(4) (the Chemical Oxygen of COD in breeding wastewater can under optimal temperature conditionss (23 DEG C -25 DEG C), be made
Demand, COD) clearance reaches 77%.
(5) after animal and fowl fodder adds compound probiotic of the present invention and environment is used cooperatively, the material of livestock and poultry is significantly reduced
Meat ratio and the incidence of disease.
Embodiment
Technical scheme is further described with reference to specific embodiment, but the present invention is not with any shape
Formula is limited to embodiment content.Test method described in embodiment is conventional method unless otherwise specified；Such as without special theory
It is bright, the reagent and biomaterial, commercially obtain.
Embodiment 1
A kind of compound probiotic, it is formulated by following strain is compound：
Lactobacillus delbrueckii 80 × 106CFU/ milliliters；
Lactobacillus bulgaricus 130 × 106CFU/ milliliters；
Lactobacillus helveticus 80 × 106CFU/ milliliters；
Lactobacillus acidophilus 180 × 106CFU/ milliliters；
Brewer's yeast 180 × 106CFU/ milliliters；
Wine yeast 80 × 106CFU/ milliliters；
Distillery yeast 80 × 106CFU/ milliliters；
Fodder yeast 180 × 106CFU/ milliliters；
Oil yeast 30 × 106CFU/ milliliters；
Upper strata yeast 30 × 106CFU/ milliliters；
Torula nigra 30 × 106CFU/ milliliters；
Film-forming yeast 30 × 106CFU/ milliliters；
Photosynthetic bacteria 80 × 106CFU/ milliliters；
Bacterial digestion 130 × 106CFU/ milliliters；
Bayesian sulfur bacteria 80 × 106CFU/ milliliters；
Nocard's bacillus 80 × 106CFU/ milliliters；
Sphaerotilus natans 30 × 106CFU/ milliliters；
Preparation method is as follows：
(1) liquid medium is prepared：50 grams of brown sugar, 50 grams of white sugar, 20 grams of glucose, 0.5 gram of vitamin C, skimmed milk power 10
Gram, 1000 grams of distilled water, it is mixed to form liquid medium；
(2) composite bacteria is prepared：It is compound in proportion after each individual plant strain is activated respectively, obtain composite bacteria；
(3) original bacteria liquid is prepared：10 grams of compound probiotic prepared by step (2) is put into prepared by 1000 grams of steps (1)
In liquid medium, in 35 DEG C of sealed fermentings 3 days, opening vessel port and smelling had sour-sweet taste to activate successfully, and now pH is 4.0, close
Preservation is closed to use as original bacteria liquid；
(4) second order fermentation nutrient solution is prepared：The mixture 9.6wt% of brown sugar and white sugar, vitamin C 0.2wt%, defatted milk
Powder 0.1wt%, urea 0.1wt%, remaining is except chlorine water, is mixed to form second order fermentation nutrient solution；The wherein matter of brown sugar and white sugar
Amount is than being 1:1；
(5) expansion is numerous obtains compound probiotic：Original bacteria liquid is added to the second order fermentation culture prepared in second order fermentation tank
In liquid, in 35 DEG C of sealed fermentings 3 days, opening vessel port and smelling had sour-sweet taste to expand numerous success, obtains compound probiotic；Now pH
For 3.0, total number of bacteria is about 5,000,000,000/milliliter.
The compound probiotic being prepared is added in feed in 3wt% ratios, and using vaporific in breeding environment
After the mode use of spray, feedstuff-meat ratio 0.22% can be reduced, reducing respiratory tract and the digestive tract bacterial infection incidence of disease reduces
76%, the death rate reduces by 5%, and plays a part of prebiotic bacterial immunity, and the incidence of disease of virosis reduces by 62%.
Embodiment 2
A kind of compound probiotic, it is formulated by following strain is compound：
Lactobacillus delbrueckii 120 × 106CFU/ milliliters；
Lactobacillus bulgaricus 170 × 106CFU/ milliliters；
Lactobacillus helveticus 120 × 106CFU/ milliliters；
Lactobacillus acidophilus 220 × 106CFU/ milliliters；
Brewer's yeast 220 × 106CFU/ milliliters；
Wine yeast 120 × 106CFU/ milliliters；
Distillery yeast 120 × 106CFU/ milliliters；
Fodder yeast 220 × 106CFU/ milliliters；
Oil yeast 70 × 106CFU/ milliliters；
Upper strata yeast 70 × 106CFU/ milliliters；
Torula nigra 70 × 106CFU/ milliliters；
Film-forming yeast 70 × 106CFU/ milliliters；
Photosynthetic bacteria 120 × 106CFU/ milliliters；
Bacterial digestion 170 × 106CFU/ milliliters；
Bayesian sulfur bacteria 120 × 106CFU/ milliliters；
Nocard's bacillus 120 × 106CFU/ milliliters；
Sphaerotilus natans 70 × 106CFU/ milliliters；
Preparation method is as follows：
(1) liquid medium is prepared：50 grams of brown sugar, 50 grams of white sugar, 20 grams of glucose, 0.5 gram of vitamin C, skimmed milk power 10
Gram, 1000 grams of distilled water, it is mixed to form liquid medium；
(2) composite bacteria is prepared：It is compound in proportion after each individual plant strain is activated respectively, obtain composite bacteria；
(3) original bacteria liquid is prepared：10 grams of compound probiotic prepared by step (2) is put into prepared by 1000 grams of steps (1)
In liquid medium, in 37 DEG C of sealed fermentings 3 days, opening vessel port and smelling had sour-sweet taste to activate successfully, and now pH is 4.0, close
Preservation is closed to use as original bacteria liquid；
(4) second order fermentation nutrient solution is prepared：The mixture 9.6wt% of brown sugar and white sugar, vitamin C 0.2wt%, defatted milk
Powder 0.1wt%, urea 0.1wt%, remaining is except chlorine water, is mixed to form second order fermentation nutrient solution；The wherein matter of brown sugar and white sugar
Amount is than being 1:1；
(5) expansion is numerous obtains compound probiotic：Original bacteria liquid is added to the second order fermentation culture prepared in second order fermentation tank
In liquid, in 35 DEG C of sealed fermentings 3 days, opening vessel port and smelling had sour-sweet taste to expand numerous success, obtains compound probiotic；Now pH
For 4.0, total number of bacteria is about 8,000,000,000/milliliter.
The compound probiotic being prepared is added in livestock breeding wastewater according to 0.1wt% ratio, in temperature 23
Under conditions of DEG C -25 DEG C, COD clearances in breeding wastewater can be made to reach 77%.
Embodiment 3
A kind of compound probiotic, it is formulated by following strain is compound：
Lactobacillus delbrueckii 100 × 106CFU/ milliliters；
Lactobacillus bulgaricus 150 × 106CFU/ milliliters；
Lactobacillus helveticus 100 × 106CFU/ milliliters；
Lactobacillus acidophilus 200 × 106CFU/ milliliters；
Brewer's yeast 200 × 106CFU/ milliliters；
Wine yeast 100 × 106CFU/ milliliters；
Distillery yeast 100 × 106CFU/ milliliters；
Fodder yeast 200 × 106CFU/ milliliters；
Oil yeast 50 × 106CFU/ milliliters；
Upper strata yeast 50 × 106CFU/ milliliters；
Torula nigra 50 × 106CFU/ milliliters；
Film-forming yeast 50 × 106CFU/ milliliters；
Photosynthetic bacteria 100 × 106CFU/ milliliters；
Bacterial digestion 150 × 106CFU/ milliliters；
Bayesian sulfur bacteria 100 × 106CFU/ milliliters；
Nocard's bacillus 100 × 106CFU/ milliliters；
Sphaerotilus natans 50 × 106CFU/ milliliters；
Preparation method is as follows：
(1) liquid medium is prepared：50 grams of brown sugar, 50 grams of white sugar, 20 grams of glucose, 0.5 gram of vitamin C, skimmed milk power 10
Gram, 1000 grams of distilled water, it is mixed to form liquid medium；
(2) composite bacteria is prepared：It is compound in proportion after each individual plant strain is activated respectively, obtain composite bacteria；
(3) original bacteria liquid is prepared：10 grams of compound probiotic kind prepared by step (2) is put into 1000 grams of steps (1) to prepare
Liquid medium in, in 37 DEG C of sealed fermentings 3 days, opening vessel port and smelling had sour-sweet taste to activate successfully, and now pH is 4.0,
Closed preservation uses as original bacteria liquid；
(4) second order fermentation nutrient solution is prepared：The mixture 9.6wt% of brown sugar and white sugar, vitamin C 0.2wt%, defatted milk
Powder 0.1wt%, urea 0.1wt%, remaining is except chlorine water, is mixed to form second order fermentation nutrient solution；The wherein matter of brown sugar and white sugar
Amount is than being 1:1；
(5) expansion is numerous obtains compound probiotic：Original bacteria liquid is added to the second order fermentation culture prepared in second order fermentation tank
In liquid, in 35 DEG C of sealed fermentings 3 days, opening vessel port and smelling had sour-sweet taste to expand numerous success, obtains compound probiotic；Now pH
For 4.0, total number of bacteria is about 8,000,000,000/milliliter.
The compound probiotic being prepared is added in feed in 3wt% ratios, and using vaporific in breeding environment
After the mode use of spray, feedstuff-meat ratio reduces by 0.28%, and respiratory tract and the digestive tract bacterial infection incidence of disease reduce by 80%, the death rate
5% is reduced, and plays a part of prebiotic bacterial immunity, the incidence of disease of virosis reduces by 62%.
Claims (6)
1. a kind of compound probiotic, it is characterised in that be formulated by following strain is compound：
Lactobacillus delbrueckii (L.delbrueckii) (80-120) × 106CFU/ milliliters；
Lactobacillus bulgaricus (L.bulgaricus) (130-170) × 106CFU/ milliliters；
Lactobacillus helveticus (L.helviticus) (80-120) × 106CFU/ milliliters；
Lactobacillus acidophilus (L.acidophilus) (180-220) × 106CFU/ milliliters；
Brewer's yeast (brewer ' syeast) (180-220) × 106CFU/ milliliters；
Wine yeast (wineyeast) (80-120) × 106CFU/ milliliters；
Distillery yeast (alcoholyeast) (80-120) × 106CFU/ milliliters；
Fodder yeast (fodderyeast) (180-220) × 106CFU/ milliliters；
Oil yeast (petroleumyeast) (30-70) × 106CFU/ milliliters；
Upper strata yeast (topyeast) (30-70) × 106CFU/ milliliters；
Torula nigra (blackyeast) (30-70) × 106CFU/ milliliters；
Film-forming yeast (filmyeast) (30-70) × 106CFU/ milliliters；
Photosynthetic bacteria (photosyntheticbacteria) (80-120) × 106CFU/ milliliters；
Bacterial digestion (Nitrifyingbacteria) (130-170) × 106CFU/ milliliters；
Bayesian sulfur bacteria (Beggiatoa) (80-120) × 106CFU/ milliliters；
Nocard's bacillus (Nocardia) (80-120) × 106CFU/ milliliters；
Sphaerotilus natans (Sphaerotilusnatans) (30-70) × 106CFU/ milliliters.
2. a kind of compound probiotic, it is characterised in that be formulated by following strain is compound：
Lactobacillus delbrueckii 100 × 106CFU/ milliliters；
Lactobacillus bulgaricus 150 × 106CFU/ milliliters；
Lactobacillus helveticus 100 × 106CFU/ milliliters；
Lactobacillus acidophilus 200 × 106CFU/ milliliters；
Brewer's yeast 200 × 106CFU/ milliliters；
Wine yeast 100 × 106CFU/ milliliters；
Distillery yeast 100 × 106CFU/ milliliters；
Fodder yeast 200 × 106CFU/ milliliters；
Oil yeast 50 × 106CFU/ milliliters；
Upper strata yeast 50 × 106CFU/ milliliters；
Torula nigra 50 × 106CFU/ milliliters；
Film-forming yeast 50 × 106CFU/ milliliters；
Photosynthetic bacteria 100 × 106CFU/ milliliters；
Bacterial digestion 150 × 106CFU/ milliliters；
Bayesian sulfur bacteria 100 × 106CFU/ milliliters；
Nocard's bacillus 100 × 106CFU/ milliliters；
Sphaerotilus natans 50 × 106CFU/ milliliters.
3. the preparation method of compound probiotic as claimed in claim 1, it is characterised in that comprise the following steps：
(1) liquid medium is prepared：50 parts of brown sugar, 50 parts of white sugar, 20 parts of glucose, 0.5 part of vitamin C, 10 parts of skimmed milk power, steam
1000 parts of distilled water, is mixed to form liquid medium；
(2) composite bacteria is prepared：It is compound in proportion after each individual plant strain is activated respectively, obtain composite bacteria；
(3) original bacteria liquid is prepared：10 parts of composite bacteria prepared by step (2) is put into the culture medium of 1000 parts of steps (1) preparations
In liquid, in 35 DEG C of -37 DEG C of sealed fermentings 3-5 days, opening vessel port and smelling had sour-sweet taste activate successfully, closed preservation conduct original
Bacterium solution uses, and the pH of the original bacteria liquid is 4.0-5.0；
(4) second order fermentation nutrient solution is prepared：Brown sugar and white sugar mixture 9.6wt%, vitamin C 0.2wt%, skimmed milk power
0.1wt%, urea 0.1wt%, remaining is except chlorine water, is mixed to form second order fermentation nutrient solution；Brown sugar and the white sugar mixture
The mass ratio of middle brown sugar and white sugar is 1:1；
(5) expansion is numerous obtains compound probiotic：Original bacteria liquid is added in the second order fermentation nutrient solution prepared in second order fermentation tank,
In 35 DEG C of -37 DEG C of sealed fermentings 3-5 days, opening vessel port and smelling had sour-sweet taste to expand numerous success, obtains compound probiotic；It is described
The pH of compound probiotic is 3.0-4.0, total number of bacteria >=5,000,000,000/milliliter.
4. compound probiotic as claimed in claim 1 is reducing the livestock and poultry incidence of disease, reduction livestock and poultry feedstuff-meat ratio, improved food conversion ratio
The application of aspect.
5. compound probiotic as claimed in claim 1 is in poultry excrement and urine disposal, livestock breeding wastewater, improvement its feeding environment side
The application in face.
6. application of the compound probiotic as claimed in claim 1 in terms of disease of alimentary tract of livestock husbandry and birds is prevented.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4317089A1 (en) * | 2022-08-03 | 2024-02-07 | Pruex Limited | Treatment of animal waste |
| EP4342854A1 (en) * | 2022-08-03 | 2024-03-27 | Pruex Limited | Treatment of animal waste |
Citations (6)
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| GB2621155A (en) * | 2022-08-03 | 2024-02-07 | Pruex Ltd | Treatment of animal waste |
| EP4342854A1 (en) * | 2022-08-03 | 2024-03-27 | Pruex Limited | Treatment of animal waste |
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