CN107699482A - A kind of equipment and application method of quick detection plant infection bacterial wilt - Google Patents
A kind of equipment and application method of quick detection plant infection bacterial wilt Download PDFInfo
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- CN107699482A CN107699482A CN201710704483.7A CN201710704483A CN107699482A CN 107699482 A CN107699482 A CN 107699482A CN 201710704483 A CN201710704483 A CN 201710704483A CN 107699482 A CN107699482 A CN 107699482A
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Abstract
The invention discloses a kind of equipment of quick detection plant infection bacterial wilt, mainly include base, solution-preparation device, culture apparatus, liquid-adding device, base is rectangular shape, solution-preparation device is fixed on the upper left corner of base top surface, liquid-adding device is fixed on the top of solution-preparation device, and culture apparatus is fixed on the right side of solution-preparation device；The equipment of the present invention prepares solid medium, Bacteria Culture is integrated, avoid because multiple manual operation is on influence caused by biochemical reaction process, from liquid feeding, mixed liquid, pH adjust, heat, go out in liquid step, streamline seal operation is carried out, sterileization degree is high, is not likely to produce pollution, the equipment application method of the present invention is simple to operate, naked eyes can rapid examination, effect is accurate, extensive for the application surface of Plants types choice.
Description
Technical field
The present invention relates to bacterial wilt detection technique field, and in particular to a kind of equipment of quick detection plant infection bacterial wilt
And application method.
Background technology
The cause of disease of bacterial wilt is Raul Salmonella, and cardinal symptom is：Initially, aerial part does not show any abnormalities the plant of phenomenon
Strain, daytime unexpected devitalization, whole overground part is withered.Cloudy day and sooner or later recovered, such as healthy tree, however, soon it
Just wither afterwards, it is very swift and violent in blue or green withered symptom, this process progress.It is that one kind is distributed widely in the torrid zone, subtropical zone and some temperature
The main reason for worldwide disease with area is the multiple kinds of crops underproduction.The brown stain first of the radicula of plant, starts to decay soon
And disappear.The sick stem close to surface location is cut, it can be found that vascular bundle is micro- brown stain, and white casse is secreted out of from the position
Dirty juice.Bacterial wilt is the Major Diseases of the solanaceous vegetables such as tomato, eggplant, capsicum, potato.Cardinal symptom is that plant withers rapidly
Listless, withered, cauline leaf still keeps green.The brown stain position of sick stem is extruded with hand, there is the discharge of milky bacterium solution.Hot and humid, again
Stubble continuous cropping, geodepression soil stick, when field ponding, soil meta-acid, inclined nitrogen fertilizer application, and the sickly look easily occurs.
Ralstonia solanacearum, it can together enter soil with diseased plant residuum.Long term survival forms source of infection.Soil moisture to its
Survival effect in soil is very big.In the alluvial soil of high humidity, it can survive up to 2~3 years, and in dry soil,
It can only survive several days.Ralstonia solanacearum, not survived in soil with resting state, but in above-mentioned disease plant or certain weeds
Rhizosphere bred.Ralstonia solanacearum of the existence in soil, wound is either by root mainly as caused by operation process
The wound infection plant caused by root-feeding insect such as tie lines worm, blue light rutelian larva, fallen ill at the conduit position of stem and root.Have
When also can by without wound radicula intrusion plant in fall ill.
At present, the methods of detection method on Ralstonia solanacearum includes traditional isolated culture, serology and molecular biology.
Traditional ralstonia solanacearum detection method is that pathogen is isolated and purified using selective medium, is then carried out a series of
Bio-chemical characteristics are diagnosed, and this method is easily influenceed by extraneous factor and sensitivity is relatively low.But the method has extensively
Plant applicability, if can be integrated in detection process, avoid the influence of artifact, establish a set of quick, sensitive, accurate
True ralstonia solanacearum detection device is very important.
The content of the invention
For above technical problem, the present invention provides that a kind of integrated level is high, high quick of the classification adaptability for plant
Detect the equipment and application method of plant infection bacterial wilt.
The technical scheme is that：A kind of equipment of quick detection plant infection bacterial wilt, mainly including base, solution
Preparation facilities, culture apparatus, liquid-adding device, described base are rectangular shape, and solution-preparation device is fixed on base top surface
The upper left corner, liquid-adding device is fixed on the top of solution-preparation device, and culture apparatus is fixed on the right side of solution-preparation device；Institute
The lower left corner for the base top surface stated is respectively arranged with temperature display, pH display screens, capacity display screen, described temperature from bottom to top
Mixed liquid switch is provided with the right side of degree display screen, is provided with draining switch on the right side of described pH display screens, described capacity shows
Heater switch is provided with the right side of display screen, power switch, the rightmost side of base front surface are provided with the right side of described heater switch
Culture fixator is provided with, the upper right corner of described culture fixator is provided with positive button and negative button, the described positive
Wireless data transmitter built in button and negative button；Being internally embedded for described solution-preparation device is provided with controller, institute
Mixed liquid container is provided with the top of the controller stated, described mixed liquid container is ball-type glass mixer for liquid ware, mixes the bottom of liquid container
Portion is provided with heater, and the bottom of described heater is provided with temperature sensor, and the bottom of mixed liquid container is provided with out
Liquid pipeline, agitator is provided with the top of heater, capacity inductor, the lower section of side wall are provided with the side wall of mixed liquid container
It is provided with pH sensors；Described liquid-adding device is up big and down small funnel shaped, and pH regulations are respectively arranged with larger face
Pipe, solid tube, liquid line, pH regulation funnels are provided with described pH regulation pipes, and the top of described pH regulation funnels is set
There is regulation liquid container, described pH regulation funnels are provided with pH regulating valves with the junction for adjusting liquid container；Described culture dress
Putting mainly includes observation window, liquid collecting funnel, water influent pipeline, culture vessel, liquid storage container, dropping funel, liquid-dropping valve, described sight
The top that window is arranged on liquid collecting funnel is examined, the outlet end of described water influent pipeline is connected with liquid collecting funnel, the feed liquor of water influent pipeline
End is connected with described drain pipe road, and described liquid collecting funnel is fixed on the top of culture vessel, the side of described culture vessel
Dropping funel is installed, described dropping funnel is connected by liquid-dropping valve with liquid storage container on wall；Described temperature display
Electrically connected between screen, pH display screens, capacity display screen, mixed liquid switch, draining switch, heater switch, power switch and controller,
Electrically connected between described heater, temperature sensor, agitator, capacity inductor, pH sensors and controller, heating dress
Put, temperature sensor, agitator, capacity inductor, pH sensors, controller are connected with external power supply respectively.
Further, described culture fixator is strip-shaped baffle, and hinge is provided with the left of described strip-shaped baffle, and
It is connected by hinge with base, fastening switch is provided with the right side of strip-shaped baffle, culture fixator is by fastening switch and base
It is fixed, culture vessel can be taken out independently of culture apparatus by opening strip-shaped baffle, it is convenient and swift, use and the side of cleaning
Just.
Further, ring shaped heating mechanism is provided with described liquid collecting funnel, described ring shaped heating mechanism is some
Heater strip, described heater strip are arranged in liquid collecting hopper side walls, and heater strip is wired to controller, in liquid collecting funnel
Temperature sense probe is provided with, described temperature sense probe is electrically connected with the controller, and can be manufactured one to culture medium and is adapted to
Environment temperature, selectivity is high, and temperature regulating range is big.
Further, described heater switch is set singly to open dual control, the control model and temperature display of heater switch
Display pattern be consistent, described control model include heater control and heater strip control two kinds, described display
Pattern includes temperature sensor temperature display and temperature sense probe temperature display, singly opens dual control selectively actuatable, and integrated level is high,
It is cost-effective.
Further, described controller is commercially available PLC, mainly including power supply, CPU, storage
Device, input/output interface circuit, functional module, communication module, the general AC voltage fluctuations of described power supply are ± 10%
In the range of, PLC is directly connected to AC network；Described CPU is PLC control axis, for receiving and depositing
Store up the user program keyed in from programmable device and data；Power supply, memory, I/O and the state for guarding against timer are checked, and can be examined
Syntax error in disconnected user program；Described memory storage system software.
A kind of application method of the equipment of quick detection plant infection bacterial wilt mainly includes the following steps that：
Step 1：First, turn on the power switch, the plasma water of unit volume is added by liquid line, then by solid
Body pipe is separately added into 0.5-1.5g/L lactoalbumin hydrolysate, 8-12g/L peptone, 4-6g/L glucose, 16-18g/L's
Agar, mixed liquid switch is then opened, is stirred mixed liquid 5-15min, after solution stirs, observed by pH display screens molten
The pH value of liquid, pH adjusting agent is added to regulation liquid container, described pH adjusting agent is 1mol/L hydrochloric acid, then rotates pH regulations
Valve, funnel is adjusted by pH and carries out pH regulations, until the pH value of solution is 6.5-7.0, heater switch is then opened, passes through temperature
Display screen controls heated solution heat sterilization 15min at a temperature of 121 DEG C, opens draining switch so that solution passes through respectively
Drain pipe road and water influent pipeline enter culture vessel, form solid medium after cooling to room temperature, standby；
Step 2：Observation window is first opened, in the solid medium for then preparing the phytopathogen injection of separator well,
Heater switch is adjusted, the cultivation temperature of solid medium is observed by temperature sense probe, is heated using heater strip so that solid
The temperature of culture medium is 35 DEG C, then cultivates phytopathogen 24-48h at this temperature；
Step 3：After the completion of culture, to 2,3, the 5- chlorinated triphenyls four that the liquor capacity 3-5% is added in liquid storage container
Nitrogen azoles, liquid-dropping valve is opened, 2,3,5- TTCs are added in solid medium using dropping funel, pass through observation window
Observation, judges whether contain Ralstonia solanacearum in described phytopathogen according to reaction color, TTC can be with green grass or young crops
Withered bacterium chemically reacts, and pathogenic bacterium colony can become the pink of white halo, the bacterium colony reaction of no pathogenicity after reacting
After can become laking, TTC/TZC (Tripheny tetrazolium choride) that Kelman worked out in 1954 training
Support base.Dedicated for separating Ralstonia solanacearum, 2,3,5- TTCs are added on the basis of CPG, are contained in the culture medium
TTC chemically reacts with Ralstonia solanacearum, so as to distinguish two kinds of Colonial types：There is white in pathogenic bacterium colony
The pink bacterium colony of haloing, the bacterium colony of no pathogenicity then show as peony, press positive button if containing Ralstonia solanacearum, do not have
There is Ralstonia solanacearum then to press negative button, detection structure is sent by wireless data transmitter.
Further, the separation of described phytopathogen is obtained using tissue isolation by sampling apparatus, institute
The sampling apparatus stated mainly includes sample holes, extruding disk, motor, water inlet, water tank, water jet, rinse tank, sample outlet hole, goes out sample
Switch, disinfect box discharging switch, discharge baffle, disinfect box, thimerosal gateway, sterilamp, described sample holes and extruding disk
Connection, described extruding disk are connected with motor by being driven axis connection, the right side of extruding disk with disinfect box, described disinfect box top
Portion is provided with sterilamp, is connected above disinfect box with thimerosal gateway, and the bottom of disinfect box is provided with discharge baffle, described
Discharge baffle is connected with disinfect box discharging switch connection, discharge baffle with rinse tank, and water spray is provided with the left of described rinse tank
Device, described water jet are connected with water tank, and described water tank is connected with water inlet, and the lower right side of rinse tank is provided with sample outlet hole,
Sample switch is provided with out on described sample outlet hole, the described sample switch that goes out is connected with squeezer, and described squeezer fills with culture
Put connection；The fritter diseased tissues for cutting the 2-3mm for treating measuring plants is put into sample holes, under the aseptic condition of sterilamp, by disappearing
The liquor natrii hypochloritis that venom gateway adds 4-6% sterilizes 5-8min, is then switched using disinfect box discharging by the small of sterilization
Block diseased tissues is put into the sterilized water of rinse tank, is rinsed 3 times, is then squeezed out phytopathogen by squeezer.
Beneficial effects of the present invention are compared with prior art：The equipment of the quick detection plant infection bacterial wilt of the present invention
It is prepared by solid medium, Bacteria Culture it is integrated, avoid because multiple manual operation to being made in biochemical reaction process
Into influence, from liquid feeding, mixed liquid, pH adjust, heat, go out in liquid step, carry out streamline seal operation, sterileization degree height,
Pollution is not likely to produce, equipment application method of the invention is simple to operate, and described phytopathogen is judged according to reaction color
In whether contain Ralstonia solanacearum, pathogenic bacterium colony can become the pink of white halo, the bacterium colony reaction of no pathogenicity after reacting
After can become laking, aberration is obvious, without using specialty facilities for observation, visually can rapid examination, effect is accurate, for
The application surface of Plants types choice is extensive.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the liquid collecting funnel structure schematic diagram of the present invention；
Fig. 3 is the electrical equipment annexation figure of the present invention；
Fig. 4 is the sampling apparatus structural representation of the present invention；
Wherein, 1- bases, 101- temperature displays, 102-pH display screens, 103- capacity display screens, 104- mix liquid switch,
105- draining switch, 106- heater switch, 107- power switches, 108- culture fixators, 1081- fastening switches, 1082- are closed
Page, 2- solution-preparation devices, 201- mix liquid container, 202- capacity inductors, 203- agitators, 204- heaters, 205- temperature
Spend sensor, 206-pH sensors, 207- drain pipes road, 208- controllers, 3- culture apparatuses, 301- observation windows, 302- liquid collectings
Funnel, 3021- heater strips, 3022- temperature sense probes, 3023- wires, 303- water influent pipelines, 304- culture vessels, 305- are deposited
Liquid container, 306- dropping funels, 307- liquid-dropping valves, 4- liquid-adding devices, 401-pH regulation pipes, 402- solid tubes, 403- liquid lines,
404-pH regulation funnels, 405-pH regulating valves, 406- regulation liquid containers, 5- sampling apparatuses, 501- sample holes, 502- extruding disks,
503- motors, 504- water inlets, 505- water tanks, 506- water jets, 507- rinse tanks, 508- sample outlet holes, 509- go out sample switch,
510- disinfect boxes discharging switch, 511- discharge baffles, 512- disinfect boxes, 513- thimerosals gateway, 514- sterilamps, 515- are squeezed
Depressor.
Embodiment
The present invention is further described in detail with reference to specific embodiment：External power supply
A kind of equipment of quick detection plant infection bacterial wilt of the present invention is used into the doubtful bacterial wilt of certain tobacco to infect
In detection, 30 plants of certain tobacco is chosen, is divided into three groups, every group 10 plants, experimental group 1, experimental group 2, experimental group 3, for three groups of experiments
The tobacco of group is detected using the equipment of embodiment 1, embodiment 2, embodiment 3 respectively, is then answered for testing result
Test, verify the accuracy of the equipment of the present invention：
Embodiment 1：As shown in figure 1, equipment mainly includes base 1, solution-preparation device 2, culture apparatus 3, liquid-adding device
4, base 1 is rectangular shape, and solution-preparation device 2 is fixed on the upper left corner of the top surface of base 1, and liquid-adding device 4 is fixed on solution
The top of preparation facilities 2, culture apparatus 3 are fixed on the right side of solution-preparation device 2；
The lower left corner of the top surface of base 1 is respectively arranged with temperature display 101, pH display screens 102, capacity and shown from bottom to top
Screen 103, the right side of temperature display 101, which is provided with mixed liquid and switchs the right sides of 104, pH display screens 102, is provided with draining switch
105, the right side of capacity display screen 103 is provided with heater switch 106, and the right side of heater switch 106 is provided with power switch 107,
The 1 positive rightmost side of base is provided with culture fixator 108, and the upper right corner of culture fixator 108 is provided with the positive He of button 109
Negative button 110, wireless data transmitter built in positive button 109 and negative button 110；Fixator 108 is cultivated to keep off for bar shaped
Plate, hinge 1082 being provided with the left of strip-shaped baffle, and being connected by hinge 1082 with base 1, the right side of strip-shaped baffle is set
There is fastening switch 1081, culture fixator 108 1081 is fixed by fastening switch with base 1, can be with by opening strip-shaped baffle
Culture vessel 304 is taken out independently of culture apparatus 3, it is convenient and swift, using with it is easy to clean.
Being internally embedded for solution-preparation device 2 is provided with controller 208, and the top of controller 208 is provided with mixed liquid container
201, it is ball-type glass mixer for liquid ware to mix liquid container 201, and the bottom of mixed liquid container 201 is provided with heater 204, heater
204 bottom is provided with temperature sensor 205, and the bottom of mixed liquid container 201 is provided with drain pipe road 207, heater 204
Top is provided with agitator 203, is provided with capacity inductor 202 in the side wall of mixed liquid container 201, pH is provided with below side wall
Sensor 206；
Liquid-adding device 4 is up big and down small funnel shaped, and pH regulation pipes 401, solid tube are respectively arranged with larger face
402nd, liquid line 403, pH regulation funnels 404 are provided with pH regulation pipes 401, and the top of pH regulation funnels 404 is provided with regulation
The junction of liquid container 406, pH regulation funnels 404 and regulation liquid container 406 is provided with pH regulating valves 405；
Culture apparatus 3 mainly includes observation window 301, liquid collecting funnel 302, water influent pipeline 303, culture vessel 304, liquid storage and held
Device 305, dropping funel 306, liquid-dropping valve 307, observation window 301 are arranged on the top of liquid collecting funnel 302, and water influent pipeline 303 goes out
Liquid end is connected with liquid collecting funnel 302, and the liquid feeding end of water influent pipeline 303 is connected with drain pipe road 207, and liquid collecting funnel 302 is fixed on
The top of culture vessel 304, dropping funel 306 is provided with the side wall of culture vessel 304, and the top of dropping funel 306 passes through drop
Liquid valve 307 is connected with liquid storage container 305；As shown in Fig. 2 ring shaped heating mechanism is provided with liquid collecting funnel 302, annular-heating dress
Heater strip 3021 is set to, heater strip 3021 is arranged in the side wall of liquid collecting funnel 302, and heater strip 3021 is connected to by wire 3023
Controller 208, temperature sense probe 3022 is provided with liquid collecting funnel 302, temperature sense probe 3022 is electrically connected with controller 208
Connect, a suitable environment temperature can be manufactured to culture medium, selectivity is high, and temperature regulating range is big.
As shown in figure 3, temperature display 101, pH display screens 102, capacity display screen 103, mixed liquid switch 104, discharge opeing and opened
Close and electrically connected between 105, heater switch 106, power switch 107 and controller 208, heater 204, temperature sensor 205,
Electrically connected between agitator 203, capacity inductor 202, pH sensors 206 and controller 208, heater 204, TEMP
Device 205, agitator 203, capacity inductor 202, pH sensors 206, controller 208 are connected with external power supply respectively；Heating is opened
106 are closed singly to open dual control setting, the control model and the display pattern of temperature display 101 of heater switch 106 are consistent, and are controlled
Molding formula includes the control of heater 204 and heater strip 3021 controls two kinds, and display pattern shows including the temperature of temperature sensor 205
Show with the temperature display of temperature sense probe 3022, singly open dual control selectively actuatable, integrated level is high, and cost-effective, controller 208 is
Commercially available PLC, mainly including power supply, CPU, memory, input/output interface circuit, functional module, logical
Believe module, PLC is directly connected to AC network by the general AC voltage fluctuations of power supply in the range of ± 10%；Centre
Reason unit is PLC control axis, for receiving and storing the user program keyed in from programmable device and data；Check power supply, deposit
Reservoir, I/O and the state for guarding against timer, and the syntax error in user program can be diagnosed；Memory storage system software.
As shown in figure 4, the separation of phytopathogen is obtained using tissue isolation by sampling apparatus 5, sampling cartridge
Put 5 mainly include sample holes 501, extruding disk 502, motor 503, water inlet 504, water tank 505, water jet 506, rinse tank 507,
Sample outlet hole 508, go out sample switch 509, disinfect box discharging switch 510, discharge baffle 511, disinfect box 512, thimerosal gateway
513rd, sterilamp 514, sample holes 501 are connected with extruding disk 502, and extruding disk 502, by being driven axis connection, extrudes with motor 503
The right side of disk 502 is connected with disinfect box 512, and the top of disinfect box 512 is provided with sterilamp 514, and disinfect box 512 is above and thimerosal
Gateway 513 connects, and the bottom of disinfect box 512 is provided with discharge baffle 511, discharge baffle 511 and disinfect box discharging switch 510
Connection, discharge baffle 511 are connected with rinse tank 507, and the left side of rinse tank 507 is provided with water jet 506, water jet 506 and water tank
505 connections, water tank 505 are connected with water inlet 504, and the lower right side of rinse tank 507 is provided with sample outlet hole 508, is set on sample outlet hole 508
Sample switch 509 is equipped with out, goes out sample switch 509 and is connected with squeezer 515, squeezer 515 is connected with culture apparatus 3.
A kind of application method of the equipment of quick detection plant infection bacterial wilt mainly includes the following steps that：
Step 1：First, 107 are turned on the power switch, the plasma water of unit volume is added by liquid line 403, then
0.5g/L lactoalbumin hydrolysate, 8g/L peptone, 4g/L glucose, 16g/L fine jade are separately added into by solid tube 402
Fat, mixed liquid switch 104 is then opened, mixed liquid 5min is stirred, after solution stirs, is observed by pH display screens 102
The pH value of solution, pH adjusting agent is added to regulation liquid container 406, pH adjusting agent is 1mol/L hydrochloric acid, then rotates pH regulations
Valve 405, funnel 404 is adjusted by pH and carries out pH regulations, until the pH value of solution is 6.5, heater switch 106 is then opened, leads to
Excess temperature display screen 101 controls heated solution heat sterilization 15min at a temperature of 121 DEG C, opens draining switch 105 so that
Solution enters culture vessel 304 by drain pipe road 207 and water influent pipeline 303 respectively, forms solid training after cooling to room temperature
Base is supported, it is standby；
Step 2：The fritter diseased tissues for cutting the 2mm for treating measuring plants is put into sample holes 501, in the sterile bar of sterilamp 514
Under part, the liquor natrii hypochloritis that 4% is added by thimerosal gateway 513 sterilizes 5min, and then being discharged using disinfect box is switched
510 are put into the fritter diseased tissues of sterilization in the sterilized water of rinse tank 507, rinse 3 times, are then squeezed out by squeezer 515
Phytopathogen；
Step 3：Observation window 301 is first opened, the solid medium for then preparing the phytopathogen injection of separator well
In, heater switch 106 is adjusted, the cultivation temperature of solid medium is observed by temperature sense probe 3022, uses heater strip
3021 heating so that the temperature of solid medium is 35 DEG C, then cultivates phytopathogen 24h at this temperature；
Step 4：After the completion of culture, to 2,3, the 5- chlorinated triphenyls that the liquor capacity 3% is added in liquid storage container 305
Tetrazole, liquid-dropping valve 307 is opened, 2,3,5- TTCs are added in solid medium using dropping funel 306, led to
Cross observation window 301 to observe, judge whether contain Ralstonia solanacearum in phytopathogen according to reaction color, TTC can
Chemically reacted with Ralstonia solanacearum, pathogenic bacterium colony can become the pink of white halo, the bacterium colony of no pathogenicity after reacting
Laking can be become after reaction, press positive button 109 if containing Ralstonia solanacearum, no Ralstonia solanacearum then presses negative button
110, detection structure is sent by wireless data transmitter.
Embodiment 2：As shown in figure 1, equipment mainly includes base 1, solution-preparation device 2, culture apparatus 3, liquid-adding device
4, base 1 is rectangular shape, and solution-preparation device 2 is fixed on the upper left corner of the top surface of base 1, and liquid-adding device 4 is fixed on solution
The top of preparation facilities 2, culture apparatus 3 are fixed on the right side of solution-preparation device 2；
The lower left corner of the top surface of base 1 is respectively arranged with temperature display 101, pH display screens 102, capacity and shown from bottom to top
Screen 103, the right side of temperature display 101, which is provided with mixed liquid and switchs the right sides of 104, pH display screens 102, is provided with draining switch
105, the right side of capacity display screen 103 is provided with heater switch 106, and the right side of heater switch 106 is provided with power switch 107,
The 1 positive rightmost side of base is provided with culture fixator 108, and the upper right corner of culture fixator 108 is provided with the positive He of button 109
Negative button 110, wireless data transmitter built in positive button 109 and negative button 110；Fixator 108 is cultivated to keep off for bar shaped
Plate, hinge 1082 being provided with the left of strip-shaped baffle, and being connected by hinge 1082 with base 1, the right side of strip-shaped baffle is set
There is fastening switch 1081, culture fixator 108 1081 is fixed by fastening switch with base 1, can be with by opening strip-shaped baffle
Culture vessel 304 is taken out independently of culture apparatus 3, it is convenient and swift, using with it is easy to clean.
Being internally embedded for solution-preparation device 2 is provided with controller 208, and the top of controller 208 is provided with mixed liquid container
201, it is ball-type glass mixer for liquid ware to mix liquid container 201, and the bottom of mixed liquid container 201 is provided with heater 204, heater
204 bottom is provided with temperature sensor 205, and the bottom of mixed liquid container 201 is provided with drain pipe road 207, heater 204
Top is provided with agitator 203, is provided with capacity inductor 202 in the side wall of mixed liquid container 201, pH is provided with below side wall
Sensor 206；
Liquid-adding device 4 is up big and down small funnel shaped, and pH regulation pipes 401, solid tube are respectively arranged with larger face
402nd, liquid line 403, pH regulation funnels 404 are provided with pH regulation pipes 401, and the top of pH regulation funnels 404 is provided with regulation
The junction of liquid container 406, pH regulation funnels 404 and regulation liquid container 406 is provided with pH regulating valves 405；
Culture apparatus 3 mainly includes observation window 301, liquid collecting funnel 302, water influent pipeline 303, culture vessel 304, liquid storage and held
Device 305, dropping funel 306, liquid-dropping valve 307, observation window 301 are arranged on the top of liquid collecting funnel 302, and water influent pipeline 303 goes out
Liquid end is connected with liquid collecting funnel 302, and the liquid feeding end of water influent pipeline 303 is connected with drain pipe road 207, and liquid collecting funnel 302 is fixed on
The top of culture vessel 304, dropping funel 306 is provided with the side wall of culture vessel 304, and the top of dropping funel 306 passes through drop
Liquid valve 307 is connected with liquid storage container 305；As shown in Fig. 2 ring shaped heating mechanism is provided with liquid collecting funnel 302, annular-heating dress
4 circle heater strips 3021 being arranged in the side wall of liquid collecting funnel 302 are set to, heater strip 3021 is connected to controller by wire 3023
208, temperature sense probe 3022 is provided with liquid collecting funnel 302, and temperature sense probe 3022 electrically connects with controller 208, can
To manufacture a suitable environment temperature to culture medium, selectivity is high, and temperature regulating range is big.
As shown in figure 3, temperature display 101, pH display screens 102, capacity display screen 103, mixed liquid switch 104, discharge opeing and opened
Close and electrically connected between 105, heater switch 106, power switch 107 and controller 208, heater 204, temperature sensor 205,
Electrically connected between agitator 203, capacity inductor 202, pH sensors 206 and controller 208, heater 204, TEMP
Device 205, agitator 203, capacity inductor 202, pH sensors 206, controller 208 are connected with external power supply respectively；Heating is opened
106 are closed singly to open dual control setting, the control model and the display pattern of temperature display 101 of heater switch 106 are consistent, and are controlled
Molding formula includes the control of heater 204 and heater strip 3021 controls two kinds, and display pattern shows including the temperature of temperature sensor 205
Show with the temperature display of temperature sense probe 3022, singly open dual control selectively actuatable, integrated level is high, and cost-effective, controller 208 is
Commercially available PLC, mainly including power supply, CPU, memory, input/output interface circuit, functional module, logical
Believe module, PLC is directly connected to AC network by the general AC voltage fluctuations of power supply in the range of ± 10%；Centre
Reason unit is PLC control axis, for receiving and storing the user program keyed in from programmable device and data；Check power supply, deposit
Reservoir, I/O and the state for guarding against timer, and the syntax error in user program can be diagnosed；Memory storage system software.
As shown in figure 4, the separation of phytopathogen is obtained using tissue isolation by sampling apparatus 5, sampling cartridge
Put 5 mainly include sample holes 501, extruding disk 502, motor 503, water inlet 504, water tank 505, water jet 506, rinse tank 507,
Sample outlet hole 508, go out sample switch 509, disinfect box discharging switch 510, discharge baffle 511, disinfect box 512, thimerosal gateway
513rd, sterilamp 514, sample holes 501 are connected with extruding disk 502, and extruding disk 502, by being driven axis connection, extrudes with motor 503
The right side of disk 502 is connected with disinfect box 512, and the top of disinfect box 512 is provided with sterilamp 514, and disinfect box 512 is above and thimerosal
Gateway 513 connects, and the bottom of disinfect box 512 is provided with discharge baffle 511, discharge baffle 511 and disinfect box discharging switch 510
Connection, discharge baffle 511 are connected with rinse tank 507, and the left side of rinse tank 507 is provided with water jet 506, water jet 506 and water tank
505 connections, water tank 505 are connected with water inlet 504, and the lower right side of rinse tank 507 is provided with sample outlet hole 508, is set on sample outlet hole 508
Sample switch 509 is equipped with out, goes out sample switch 509 and is connected with squeezer 515, squeezer 515 is connected with culture apparatus 3.
A kind of application method of the equipment of quick detection plant infection bacterial wilt mainly includes the following steps that：
Step 1：First, 107 are turned on the power switch, the plasma water of unit volume is added by liquid line 403, then
It is separately added into 1g/L lactoalbumin hydrolysate by solid tube 402,10g/L peptone, 5g/L glucose, 17g/L agar,
Then mixed liquid switch 104 is opened, mixed liquid 10min is stirred, after solution stirs, is observed by pH display screens 102 molten
The pH value of liquid, pH adjusting agent is added to regulation liquid container 406, pH adjusting agent is 1mol/L hydrochloric acid, then rotates pH regulating valves
405, funnel 404 is adjusted by pH and carries out pH regulations, until the pH value of solution is 6.75, heater switch 106 is then opened, passes through
Temperature display 101 controls heated solution heat sterilization 15min at a temperature of 121 DEG C, opens draining switch 105 so that molten
Liquid enters culture vessel 304 by drain pipe road 207 and water influent pipeline 303 respectively, forms solid culture after cooling to room temperature
Base, it is standby；
Step 2：The fritter diseased tissues for cutting the 2.5mm for treating measuring plants is put into sample holes 501, in the sterile of sterilamp 514
Under the conditions of, the liquor natrii hypochloritis that 5% is added by thimerosal gateway 513 sterilizes 6.5min, is then discharged using disinfect box
The fritter diseased tissues of sterilization is put into the sterilized water of rinse tank 507 by switch 510, is rinsed 3 times, is then squeezed by squeezer 515
Extrude phytopathogen；
Step 3：Observation window 301 is first opened, the solid medium for then preparing the phytopathogen injection of separator well
In, heater switch 106 is adjusted, the cultivation temperature of solid medium is observed by temperature sense probe 3022, uses heater strip
3021 heating so that the temperature of solid medium is 35 DEG C, then cultivates phytopathogen 36h at this temperature；
Step 4：After the completion of culture, to 2,3, the 5- chlorinated triphenyls that the liquor capacity 4% is added in liquid storage container 305
Tetrazole, liquid-dropping valve 307 is opened, 2,3,5- TTCs are added in solid medium using dropping funel 306, led to
Cross observation window 301 to observe, judge whether contain Ralstonia solanacearum in phytopathogen according to reaction color, TTC can
Chemically reacted with Ralstonia solanacearum, pathogenic bacterium colony can become the pink of white halo, the bacterium colony of no pathogenicity after reacting
Laking can be become after reaction, press positive button 109 if containing Ralstonia solanacearum, no Ralstonia solanacearum then presses negative button
110, detection structure is sent by wireless data transmitter.
Embodiment 3：As shown in figure 1, equipment mainly includes base 1, solution-preparation device 2, culture apparatus 3, liquid-adding device
4, base 1 is rectangular shape, and solution-preparation device 2 is fixed on the upper left corner of the top surface of base 1, and liquid-adding device 4 is fixed on solution
The top of preparation facilities 2, culture apparatus 3 are fixed on the right side of solution-preparation device 2；
The lower left corner of the top surface of base 1 is respectively arranged with temperature display 101, pH display screens 102, capacity and shown from bottom to top
Screen 103, the right side of temperature display 101, which is provided with mixed liquid and switchs the right sides of 104, pH display screens 102, is provided with draining switch
105, the right side of capacity display screen 103 is provided with heater switch 106, and the right side of heater switch 106 is provided with power switch 107,
The 1 positive rightmost side of base is provided with culture fixator 108, and the upper right corner of culture fixator 108 is provided with the positive He of button 109
Negative button 110, wireless data transmitter built in positive button 109 and negative button 110；Fixator 108 is cultivated to keep off for bar shaped
Plate, hinge 1082 being provided with the left of strip-shaped baffle, and being connected by hinge 1082 with base 1, the right side of strip-shaped baffle is set
There is fastening switch 1081, culture fixator 108 1081 is fixed by fastening switch with base 1, can be with by opening strip-shaped baffle
Culture vessel 304 is taken out independently of culture apparatus 3, it is convenient and swift, using with it is easy to clean.
Being internally embedded for solution-preparation device 2 is provided with controller 208, and the top of controller 208 is provided with mixed liquid container
201, it is ball-type glass mixer for liquid ware to mix liquid container 201, and the bottom of mixed liquid container 201 is provided with heater 204, heater
204 bottom is provided with temperature sensor 205, and the bottom of mixed liquid container 201 is provided with drain pipe road 207, heater 204
Top is provided with agitator 203, is provided with capacity inductor 202 in the side wall of mixed liquid container 201, pH is provided with below side wall
Sensor 206；
Liquid-adding device 4 is up big and down small funnel shaped, and pH regulation pipes 401, solid tube are respectively arranged with larger face
402nd, liquid line 403, pH regulation funnels 404 are provided with pH regulation pipes 401, and the top of pH regulation funnels 404 is provided with regulation
The junction of liquid container 406, pH regulation funnels 404 and regulation liquid container 406 is provided with pH regulating valves 405；
Culture apparatus 3 mainly includes observation window 301, liquid collecting funnel 302, water influent pipeline 303, culture vessel 304, liquid storage and held
Device 305, dropping funel 306, liquid-dropping valve 307, observation window 301 are arranged on the top of liquid collecting funnel 302, and water influent pipeline 303 goes out
Liquid end is connected with liquid collecting funnel 302, and the liquid feeding end of water influent pipeline 303 is connected with drain pipe road 207, and liquid collecting funnel 302 is fixed on
The top of culture vessel 304, dropping funel 306 is provided with the side wall of culture vessel 304, and the top of dropping funel 306 passes through drop
Liquid valve 307 is connected with liquid storage container 305；As shown in Fig. 2 ring shaped heating mechanism is provided with liquid collecting funnel 302, annular-heating dress
5 circle heater strips 3021 being arranged in the side wall of liquid collecting funnel 302 are set to, heater strip 3021 is connected to controller by wire 3023
208, temperature sense probe 3022 is provided with liquid collecting funnel 302, and temperature sense probe 3022 electrically connects with controller 208, can
To manufacture a suitable environment temperature to culture medium, selectivity is high, and temperature regulating range is big.
As shown in figure 3, temperature display 101, pH display screens 102, capacity display screen 103, mixed liquid switch 104, discharge opeing and opened
Close and electrically connected between 105, heater switch 106, power switch 107 and controller 208, heater 204, temperature sensor 205,
Electrically connected between agitator 203, capacity inductor 202, pH sensors 206 and controller 208, heater 204, TEMP
Device 205, agitator 203, capacity inductor 202, pH sensors 206, controller 208 are connected with external power supply respectively；Heating is opened
106 are closed singly to open dual control setting, the control model and the display pattern of temperature display 101 of heater switch 106 are consistent, and are controlled
Molding formula includes the control of heater 204 and heater strip 3021 controls two kinds, and display pattern shows including the temperature of temperature sensor 205
Show with the temperature display of temperature sense probe 3022, singly open dual control selectively actuatable, integrated level is high, and cost-effective, controller 208 is
Commercially available PLC, mainly including power supply, CPU, memory, input/output interface circuit, functional module, logical
Believe module, PLC is directly connected to AC network by the general AC voltage fluctuations of power supply in the range of ± 10%；Centre
Reason unit is PLC control axis, for receiving and storing the user program keyed in from programmable device and data；Check power supply, deposit
Reservoir, I/O and the state for guarding against timer, and the syntax error in user program can be diagnosed；Memory storage system software.
As shown in figure 4, the separation of phytopathogen is obtained using tissue isolation by sampling apparatus 5, sampling cartridge
Put 5 mainly include sample holes 501, extruding disk 502, motor 503, water inlet 504, water tank 505, water jet 506, rinse tank 507,
Sample outlet hole 508, go out sample switch 509, disinfect box discharging switch 510, discharge baffle 511, disinfect box 512, thimerosal gateway
513rd, sterilamp 514, sample holes 501 are connected with extruding disk 502, and extruding disk 502, by being driven axis connection, extrudes with motor 503
The right side of disk 502 is connected with disinfect box 512, and the top of disinfect box 512 is provided with sterilamp 514, and disinfect box 512 is above and thimerosal
Gateway 513 connects, and the bottom of disinfect box 512 is provided with discharge baffle 511, discharge baffle 511 and disinfect box discharging switch 510
Connection, discharge baffle 511 are connected with rinse tank 507, and the left side of rinse tank 507 is provided with water jet 506, water jet 506 and water tank
505 connections, water tank 505 are connected with water inlet 504, and the lower right side of rinse tank 507 is provided with sample outlet hole 508, is set on sample outlet hole 508
Sample switch 509 is equipped with out, goes out sample switch 509 and is connected with squeezer 515, squeezer 515 is connected with culture apparatus 3.
A kind of application method of the equipment of quick detection plant infection bacterial wilt mainly includes the following steps that：
Step 1：First, 107 are turned on the power switch, the plasma water of unit volume is added by liquid line 403, then
1.5g/L lactoalbumin hydrolysate, 12g/L peptone, 6g/L glucose, 18g/L fine jade are separately added into by solid tube 402
Fat, mixed liquid switch 104 is then opened, mixed liquid 15min is stirred, after solution stirs, is observed by pH display screens 102
The pH value of solution, pH adjusting agent is added to regulation liquid container 406, pH adjusting agent is 1mol/L hydrochloric acid, then rotates pH regulations
Valve 405, funnel 404 is adjusted by pH and carries out pH regulations, until the pH value of solution is 7.0, heater switch 106 is then opened, leads to
Excess temperature display screen 101 controls heated solution heat sterilization 15min at a temperature of 121 DEG C, opens draining switch 105 so that
Solution enters culture vessel 304 by drain pipe road 207 and water influent pipeline 303 respectively, forms solid training after cooling to room temperature
Base is supported, it is standby；
Step 2：The fritter diseased tissues for cutting the 3mm for treating measuring plants is put into sample holes 501, in the sterile bar of sterilamp 514
Under part, the liquor natrii hypochloritis that 6% is added by thimerosal gateway 513 sterilizes 8min, and then being discharged using disinfect box is switched
510 are put into the fritter diseased tissues of sterilization in the sterilized water of rinse tank 507, rinse 3 times, are then squeezed out by squeezer 515
Phytopathogen；
Step 3：Observation window 301 is first opened, the solid medium for then preparing the phytopathogen injection of separator well
In, heater switch 106 is adjusted, the cultivation temperature of solid medium is observed by temperature sense probe 3022, uses heater strip
3021 heating so that the temperature of solid medium is 35 DEG C, then cultivates phytopathogen 48h at this temperature；
Step 4：After the completion of culture, to 2,3, the 5- chlorinated triphenyls that the liquor capacity 5% is added in liquid storage container 305
Tetrazole, liquid-dropping valve 307 is opened, 2,3,5- TTCs are added in solid medium using dropping funel 306, led to
Cross observation window 301 to observe, judge whether contain Ralstonia solanacearum in phytopathogen according to reaction color, TTC can
Chemically reacted with Ralstonia solanacearum, pathogenic bacterium colony can become the pink of white halo, the bacterium colony of no pathogenicity after reacting
Laking can be become after reaction, press positive button 109 if containing Ralstonia solanacearum, no Ralstonia solanacearum then presses negative button
110, detection structure is sent by wireless data transmitter.
For three groups of experimental groups pathogenic bacteria except using the present invention apparatus and method detect in addition to, also used enzyme respectively
Connection immunoabsorption and polymerase chain reaction technology are detected, and the obtained tobacco strain number result with bacterial wilt is as follows：
| Experimental group 1 | Experimental group 2 | Experimental group 3 | Average Accuracy % | |
| The equipment of the present invention | 10 plants | 10 plants | 10 plants | 100.0 |
| Enzyme-linked Immunosorbent Assay | 8 plants | 9 plants | 9 plants | 83.3 |
| PCR | 9 plants | 9 plants | 8 plants | 83.3 |
As seen from the above table, equipment of the invention and application method are 100% for the Average Accuracy that bacterial wilt detects, can
To be widely used in plant-bacterial-wilt detection technique field.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that：It still may be used
To be modified to the technical scheme described in previous embodiment, or equivalent substitution is carried out to which part technical characteristic；And
These modifications are replaced, and the essence of appropriate technical solution is departed from the spirit and model of technical scheme of the embodiment of the present invention
Enclose.
Claims (6)
- A kind of 1. equipment of quick detection plant infection bacterial wilt, it is characterised in that described equipment mainly include base (1), Solution-preparation device (2), culture apparatus (3), liquid-adding device (4), described base (1) are rectangular shape, and solution prepares dress The upper left corner that (2) are fixed on base (1) top surface is put, liquid-adding device (4) is fixed on the top of solution-preparation device (2), culture dress Put the right side that (3) are fixed on solution-preparation device (2)；The lower left corner of described base (1) top surface is respectively arranged with from bottom to top Temperature display (101), pH display screens (102), capacity display screen (103), the right side of described temperature display (101) are set There is mixed liquid switch (104), draining switch (105), described capacity display screen are provided with the right side of described pH display screens (102) (103) heater switch (106) is provided with the right side of, power switch (107) is provided with the right side of described heater switch (106), Base (1) positive rightmost side is provided with culture fixator (108), and the upper right corner of described culture fixator (108) is provided with Positive button (109) and negative button (110), wireless data built in described positive button (109) and negative button (110) are sent out Emitter；Being internally embedded for described solution-preparation device (2) is provided with controller (208), the top of described controller (208) Mixed liquid container (201) is provided with, described mixed liquid container (201) is ball-type glass mixer for liquid ware, mixes the bottom of liquid container (201) Heater (204) is provided with, the bottom of described heater (204) is provided with temperature sensor (205), mixes liquid container (201) bottom is provided with drain pipe road (207), and agitator (203) is provided with the top of heater (204), mixes liquid container (201) capacity inductor (202) is provided with side wall, pH sensors (206) are provided with below side wall；Described liquid feeding dress It is up big and down small funnel shaped to put (4), and pH regulation pipes (401), solid tube (402), liquid are respectively arranged with larger face Manage (403), pH regulation funnels (404), the top of described pH regulation funnels (404) are provided with described pH regulation pipes (401) Portion is provided with regulation liquid container (406), and described pH adjusts funnel (404) and the junction of regulation liquid container (406) is provided with PH regulating valves (405)；Described culture apparatus (3) mainly includes observation window (301), liquid collecting funnel (302), water influent pipeline (303), culture vessel (304), liquid storage container (305), dropping funel (306), liquid-dropping valve (307), described observation window (301) Installed in the top of liquid collecting funnel (302), the outlet end of described water influent pipeline (303) is connected with liquid collecting funnel (302), feed liquor The liquid feeding end of pipeline (303) is connected with described drain pipe road (207), and described liquid collecting funnel (302) is fixed on culture vessel (304) top, dropping funel (306), described dropping funel are installed in the side wall of described culture vessel (304) (306) top is connected by liquid-dropping valve (307) with liquid storage container (305)；Described temperature display (101), pH display screens (102), capacity display screen (103), mixed liquid switch (104), draining switch (105), heater switch (106), power switch (107) Electrically connected between controller (208), described heater (204), temperature sensor (205), agitator (203), capacity Electrically connected between inductor (202), pH sensors (206) and controller (208), heater (204), temperature sensor (205), agitator (203), capacity inductor (202), pH sensors (206), controller (208) connect with external power supply respectively Connect.
- A kind of 2. equipment of quick detection plant infection bacterial wilt as claimed in claim 1, it is characterised in that described culture Fixator (108) is strip-shaped baffle, is provided with hinge (1082) on the left of described strip-shaped baffle, and by hinge (1082) with Base (1) is connected, and fastening switch (1081) is provided with the right side of strip-shaped baffle, and culture fixator (108) is switched by fastening (1081) it is fixed with base (1).
- A kind of 3. equipment of quick detection plant infection bacterial wilt as claimed in claim 1, it is characterised in that described liquid collecting It is provided with ring shaped heating mechanism on funnel (302), described ring shaped heating mechanism is some heater strips (3021), described heating Silk (3021) is arranged in liquid collecting funnel (302) side wall, and heater strip (3021) is connected to controller by wire (3023) (208) temperature sense probe (3022), described temperature sense probe (3022) and control, are provided with liquid collecting funnel (302) Device (208) electrically connects, and culture fixator (108) is fixed with base (1) by fastening switch (1081).
- A kind of 4. equipment of quick detection plant infection bacterial wilt as claimed in claim 1, it is characterised in that described heating Switch (106) singly to open dual control setting, with the display pattern of temperature display (101) protect by the control model of heater switch (106) Holding consistent, described control model includes two kinds of heater (204) control and heater strip (3021) control, described display mould Formula includes temperature sensor (205) temperature display and temperature sense probe (3022) temperature display.
- A kind of 5. application method of the equipment of quick detection plant infection bacterial wilt as claimed in claim 1, it is characterised in that Mainly include the following steps that：Step 1：First, (107) are turned on the power switch, the plasma water of unit volume are added by liquid line (403), then The peptone of about 0.5-1.5g/L lactoalbumin hydrolysate, 8-12g/L or so is separately added into by solid tube (402), 4-6g/L's Glucose, 16-18g/L agar, mixed liquid switch (104) is then opened, is stirred mixed liquid 5-15min, treat that solution stirring is equal After even, the pH value of solution is observed by pH display screens (102), pH adjusting agent is added to regulation liquid container (406), then rotated PH regulating valves (405), funnel (404) is adjusted by pH and carries out pH regulations, until the pH value of solution is 6.5-7.0 or so, then Heater switch (106) is opened, heated solution heat sterilization at a temperature of 121 DEG C is controlled by temperature display (101) 15min, open draining switch (105) so that solution enters culture by drain pipe road (207) and water influent pipeline (303) respectively Container (304), forms solid medium after cooling to room temperature, standby；Step 2：Observation window (301) is first opened, the solid medium for then preparing the phytopathogen injection of separator well In, heater switch (106) is adjusted, the cultivation temperature of solid medium is observed by temperature sense probe (3022), uses heating Silk (3021) heating so that the temperature of solid medium is 35 DEG C, then cultivates phytopathogen 24-48h at this temperature；Step 3：After the completion of culture, to 2,3, the 5- chlorinated triphenyls that the liquor capacity 3-5% is added in liquid storage container (305) Tetrazole, liquid-dropping valve (307) is opened, 2,3,5- TTCs are added into solid medium using dropping funel (306) In, observed by observation window (301), judge whether contain Ralstonia solanacearum in described phytopathogen according to reaction color, such as Fruit, which contains Ralstonia solanacearum, then presses positive button (109), and no Ralstonia solanacearum then presses negative button (110), and detection structure is passed through Wireless data transmitter is sent.
- A kind of 6. application method of the equipment of quick detection plant infection bacterial wilt as claimed in claim 5, it is characterised in that The separation of described phytopathogen is obtained using tissue isolation by sampling apparatus (5), described sampling apparatus (5) Mainly include sample holes (501), extruding disk (502), motor (503), water inlet (504), water tank (505), water jet (506), Rinse tank (507), sample outlet hole (508), go out sample switch (509), disinfect box discharging switch (510), discharge baffle (511), sterilization Case (512), thimerosal gateway (513), sterilamp (514), squeezer (515), described sample holes (501) and extruding disk (502) connect, described extruding disk (502) is with motor (503) by being driven axis connection, the right side of extruding disk (502) and sterilization Case (512) connects, and is provided with sterilamp (514) at the top of described disinfect box (512), disinfect box (512) goes out with thimerosal above Entrance (513) connects, and the bottom of disinfect box (512) is provided with discharge baffle (511), described discharge baffle (511) and sterilization Case discharging switch (510) connection, discharge baffle (511) are connected with rinse tank (507), are set on the left of described rinse tank (507) There is water jet (506), described water jet (506) is connected with water tank (505), and described water tank (505) connects with water inlet (504) Connect, the lower right side of rinse tank (507) is provided with sample outlet hole (508), and sample switch is provided with out on described sample outlet hole (508) (509), the described sample switch (509) that goes out is connected with squeezer (515), and described squeezer (515) connects with culture apparatus (3) Connect.
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| CN203021550U (en) * | 2013-01-16 | 2013-06-26 | 河北农业大学 | Fungi inoculation device of grape fruits |
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| CN101893589A (en) * | 2010-06-29 | 2010-11-24 | 中国人民解放军第三○二医院 | Sterility test method and totally closed bacteria collection ampoule incubator used thereby |
| CN203021550U (en) * | 2013-01-16 | 2013-06-26 | 河北农业大学 | Fungi inoculation device of grape fruits |
| CN107211861A (en) * | 2017-07-04 | 2017-09-29 | 四川省农业科学院经济作物育种栽培研究所 | The method of seedling stage Rapid identification tobacco bred Resistance to bacterial wilt |
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Effective date of registration: 20230606 Address after: 276000 intersection of Yucai Road and Mengshan Avenue, Lanshan District, Linyi City, Shandong Province Patentee after: Linyi xinshifeng Seed Industry Co.,Ltd. Address before: 276000 West side of north section of Industrial Avenue, mountainous area, Linyi City, Shandong Province Patentee before: LINYI University |