CN107693575A - Chinese medicine composition of the peptide containing zaocys dhumnade with dispelling wind and removing obstruction in the meridians effect and its production and use - Google Patents
Chinese medicine composition of the peptide containing zaocys dhumnade with dispelling wind and removing obstruction in the meridians effect and its production and use Download PDFInfo
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- CN107693575A CN107693575A CN201711068502.8A CN201711068502A CN107693575A CN 107693575 A CN107693575 A CN 107693575A CN 201711068502 A CN201711068502 A CN 201711068502A CN 107693575 A CN107693575 A CN 107693575A
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Classifications
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- A—HUMAN NECESSITIES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
Abstract
The invention provides a kind of Chinese medicine composition of the peptide containing zaocys dhumnade with dispelling wind and removing obstruction in the meridians effect and its production and use.The composition is made up of the following raw material medicine：The parts by weight of zaocys dhumnade 60 90, the parts by weight of the root of Dahurain angelica 5 30, and the parts by weight of perilla seed 5 30.The Chinese medicine composition dispelling wind and removing obstruction in the meridians effect of the present invention is notable.
Description
Technical field
The invention belongs to biological technical field, and in particular to Chinese medicine composition of a peptide containing zaocys dhumnade and preparation method thereof and
Purposes.
Background technology
Zaocys dhumnade is commonly called as black snake, zaocys dhumnades, is the larger nonpoisonous snake of the Colubridae zaocys dhumnade category bodily form.The effect of zaocys dhumnade with
Effect：Wind-damp dispelling, degrading the channel.Control rheumatoid arthritis stubborn, skin numbness, bone, joint tuberculosis, rubella mange, leprosy, lockjaw, children
Benumb disease.Research shows that alcohol-soluble and water soluble part can improve mouse pain threshold in Zaocys extract, and suppressing acetic acid stimulates
Pain reaction caused by abdominal cavity mucous membrane, reduce writhing number, and abdominal cavity caused by paraxylene induced mice auricle edema and acetic acid
Capillary permeability, which increases, also has obvious inhibiting effect, and in a certain amount effect relation；However, by contrast, zaocys dhumnade
Water soluble part substantially has more preferable above-mentioned drug activity compared with alcohol-soluble position again, shows, zaocys dhumnade drug activity is polarity
Larger material.In clinical practice, zaocys dhumnade is usually used in the treatment of rheumatoid arthritis, can mitigate arthritis well
Bitterly, arthritis are alleviated.Zaocys dhumnade is clinically used as medicine frequently with decocting and steeping in wine method.We pass through the side of modern pharmacology
Method research shows that Zaocys extract alcohol-soluble and water soluble part all have good anti-inflammatory pain-stopping effect, this and clinical effectiveness
Unanimously, the clinical practice for zaocys dhumnade provides the foundation of science.
Multiple studies have shown that, zaocys dhumnade peptide has the multiple functions such as antibacterial, anti-oxidant, immunological regulation, hypotensive in recent years
Activity.But how during zaocys dhumnade active peptide is prepared its sensitization and bitter taste are reduced, while farthest retain
The physiologically active of active peptide, the yield for improving zaocys dhumnade active peptide and its content of albumen and peptide are urgent problems to be solved.
The content of the invention
An object of the present disclosure is to provide a kind of Chinese medicine composition with dispelling wind and removing obstruction in the meridians effect, and it, which has, significantly dispels
Wind and removing obstruction in channels acts on.
Another object of the present invention is to provide the preparation method of above-mentioned Chinese medicine composition.
It is still another object of the present invention to provide a kind of Chinese medicine preparation with dispelling wind and removing obstruction in the meridians effect.
A further object of the present invention is the purposes for providing the Chinese medicine composition.
The Chinese medicine composition with dispelling wind and removing obstruction in the meridians effect of the present invention, is made up of the following raw material medicine：
Zaocys dhumnade 60-90 parts by weight,
Root of Dahurain angelica 5-30 parts by weight, and
Perilla seed 5-30 parts by weight.
Preparation in accordance with the present invention, it is preferable that the Chinese medicine composition is made up of the following raw material medicine：
Zaocys dhumnade 70-80 parts by weight,
Root of Dahurain angelica 10-20 parts by weight, and
Perilla seed 10-20 parts by weight.
The preparation method of Chinese medicine composition according to above-mentioned any one, it is characterised in that by the zaocys dhumnade, in vain
The root of Dahurian angelica and perilla seed powder the concentration of gained extract solution, are dried by the use of water as solvent extraction, obtain the Chinese medicine composition.
The preparation method of Chinese medicine composition according to above-mentioned any one, it is characterised in that it comprises the following steps：
A, dry zaocys dhumnade is crushed, obtains Ophidia black snake powder；The Ophidia black snake powder is added into water, adjusts pH to 8~9, immersion
12~15 hours, separation of solid and liquid, take supernatant；
B, supernatant regulation pH to 4~6 is precipitated, separation of solid and liquid, takes solid portion；
C, the solid portion is added into water, 20~40min is heated at 85~100 DEG C, homogeneous is then carried out, is mixed
Suspension；
D, the suspension is adjusted into pH to 5~9, adds enzyme preparation and digested；The enzyme preparation by flavor protease,
Trypsase and pepsin composition, and the weight ratio of the flavor protease, trypsase and pepsin is 1~3: 1~2
: 2~3；Hydrolysis temperature is 45~65 DEG C, and enzymolysis time is 2~5h, obtains enzymolysis liquid；
E, after enzymolysis terminates, the root of Dahurain angelica and perilla seed are added, extracts enzyme deactivation.The root of Dahurain angelica and perilla seed are added into the enzyme
Solve in liquid, heating and refluxing extraction, filtering, obtain mixed liquor；
F, by mixed liquor centrifugation, concentration, spray drying, the Chinese medicine composition is obtained.
According to the preparation method of described Chinese medicine composition, it is characterised in that in step B, using weak acid by the supernatant
Liquid adjusts pH to 4~6；The weak acid is selected from least one of acetic acid, lactic acid, citric acid.
According to the preparation method of described Chinese medicine composition, it is characterised in that in step E, it is described propose enzyme deactivation condition be：
Enzyme-removal temperature is 95~125 DEG C, and the enzyme deactivation time is 0.5-3h.
According to the preparation method of described Chinese medicine composition, it is characterised in that in step F, after the destroy the enzyme treatment
Enzymolysis liquid is centrifuged, and centrifugate filters through plate and frame filter press first, then through ceramic membrane filter, obtains the transmission containing active peptide
Liquid；The permeate containing active peptide is concentrated, spray drying, so as to obtain the Chinese medicine composition；The spray drying exists
Carried out at 80~105 DEG C；
According to the preparation method of described Chinese medicine composition, it is characterised in that in step F, the plate and frame filter press includes
Have a combination filtration adjuvant, the combination filtration adjuvant by activated carbon, perlite and diatomite according to weight ratio 5~6: 1~2: 1~
2 compositions.
The present invention also provides a kind of Chinese medicine preparation with dispelling wind and removing obstruction in the meridians effect, it is characterised in that the Chinese medicine preparation is adopted
Chinese medicine composition is prepared in aforementioned manners.
Chinese medicine composition produced by the present invention is used to prepare the purposes of medicine or health food with dispelling wind and removing obstruction in the meridians effect.
The preparation method technique of the present invention is simple, and cost is cheap, and the full constituent of zaocys dhumnade is utilized, gained crow
Tip snake active peptide content of peptides is high, and whole good in taste, bitterness value is relatively low, and adds the root of Dahurain angelica and perilla seed, further enhancing
The effect of this Chinese medicine composition, there is the effect of dispelling wind and removing obstruction in the meridians, invigorating the spleen and stomach.
Embodiment
With reference to specific embodiment, the present invention is further illustrated, but protection scope of the present invention is not limited to
This.
<Chinese medicine composition>
The present invention provides a kind of Chinese medicine composition of the peptide containing zaocys dhumnade with dispelling wind and removing obstruction in the meridians effect, it is characterised in that institute
Composition is stated to be made up of the following raw material medicine：
Zaocys dhumnade 60-90 parts by weight,
Root of Dahurain angelica 5-30 parts by weight, and
Perilla seed 5-30 parts by weight.
Preferably, according to above-mentioned Chinese medicine composition, it is characterised in that the composition is made up of the following raw material medicine：
Zaocys dhumnade 70-80 parts by weight,
Root of Dahurain angelica 10-20 parts by weight, and
Perilla seed 10-20 parts by weight.
For further, according to described Chinese medicine composition, it is characterised in that the composition is by the following raw material medicine system
Into：
The parts by weight of zaocys dhumnade 80, the parts by weight of the root of Dahurain angelica 10, and the parts by weight of perilla seed 10；Or
The parts by weight of zaocys dhumnade 75, the parts by weight of the root of Dahurain angelica 13, and the parts by weight of perilla seed 12；Or
The parts by weight of zaocys dhumnade 77, the parts by weight of the root of Dahurain angelica 11, and the parts by weight of perilla seed 12.
In the present invention, zaocys dhumnade peptide, the root of Dahurain angelica and perilla seed be respectively provided with very good dispelling wind and removing obstruction in the meridians, the effect of.Zaocys dhumnade
Peptide is more favorable for digesting and assimilating compared to zaocys dhumnade, and effect is more obvious, can equally be used with perilla seed, the root of Dahurain angelica.Combine herein
In thing, zaocys dhumnade enzymolysis be zaocys dhumnade peptide by we, and it is small-molecule substance and polypeptide that large protein, which is digested, beneficial to our human bodies
Intestinal absorption, and the effect of played peptide.
Matched according to the composition of the present invention, zaocys dhumnade peptide gives full play to its effect, the root of Dahurain angelica and perilla seed are made as main material
Added for auxiliary, further play its effect, and the mouthfeel and bitter taste of composition can be changed.
The root of Dahurain angelica：It is warm-natured, acrid flavour, gas fragrance, slight bitter.The root of Dahurain angelica has multiple efficacies, and a small amount of angelicotoxins are to animal oblongata blood
Pipe motor center, respiratory center, vagus nerve and spinal cord have excitation, blood pressure can be made to increase, and pulse is slack-off, and breathing adds
It is deep, and salivation can be caused to vomit；Extensive application can cause tetanic intermittent cramp, then general paralysis.Angelica anomala water decoction pair
It is various to have certain inhibitory action, there is significant inhibitory action to L-type tuberculosis.The root of Dahurain angelica is in vitro to acrothesium floccosum, stone
The fungies such as cream appearance gemma tinea bacterium have inhibitory action, and flooding agent (1: 3) also has certain suppression to Austria Du sub- gemma tinea bacterium of Ang Shi
Effect.The root of Dahurain angelica has hypotensive activity, and its contained Isomperatorin and baelfruit have hypotensive activity to cat, and action time maintains
1.5 hour.The root of Dahurain angelica has photosensitization, the furocoumarin class chemical combination such as bergapten, xanthotoxin, Isomperatorin contained by it
Thing is " optical active substance ", available for treating leucoderma.The root of Dahurain angelica plays the role of to activate sympathetic system hormone, suppresses Fatty synthesis.
The root of Dahurain angelica has anastalsis.The root of Dahurain angelica has certain effect to skin.Root of Dahurain angelica tincture external application adds low dose of UVA Radiation, to cavy two
Nitro-chlorobenzene allergic contact dermatitis has obvious inhibiting effect.The root of Dahurain angelica can suppress hepatic drug metabolizing enzyme system, and the Yunnan root of Dahurain angelica has flat
Asthma acts on.Perilla seed：Chinese medicine perilla seed is the dry mature fruit that Labiatae annual herb plant is tightly revived.Property it is pungent, temperature.Return
Lung, large intestine channel.Function lowering the adverse-rising QI to resolve phlegm, it is relieving cough and asthma, relax bowel.Cure mainly the diseases such as uncomfortable in chest, cough, constipation.The extraction of purple perilla
Thing --- Rosmarinic acid, free radical antiphlogistic effects are dispelled with extraordinary.Performance：Obtain U.S. FDA and be recognized as public's peace
Full raw-food material.Active high, species is more, available for nutrition science food and special consumable products.Function：It is inoxidizability, disease-resistant
Cytotoxic activity, antiinflammatory action, antithrombotic, platelet aggregation-against, antibacterial.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
The present invention is further detailed explanation below.
<Preparation method>
According to the first embodiment of the present invention, the preparation method of the Chinese medicine composition includes：By the zaocys dhumnade,
The root of Dahurain angelica and perilla seed powder the concentration of gained extract solution, are dried by the use of water as solvent extraction, obtain the Chinese medicine composition.
According to the first embodiment of the present invention, the preparation method of the Chinese medicine composition comprises the following steps：
A, dry zaocys dhumnade is crushed, obtains Ophidia black snake powder；The Ophidia black snake powder is added into water, adjusts pH to 8~9, immersion
12~15 hours, separation of solid and liquid, take supernatant；
B, supernatant regulation pH to 4~6 is precipitated, separation of solid and liquid, takes solid portion；
C, the solid portion is added into water, 20~40min is heated at 85~100 DEG C, homogeneous is then carried out, is mixed
Suspension；
D, the suspension is adjusted into pH to 5~9, adds enzyme preparation and digested；The enzyme preparation by flavor protease,
Trypsase and pepsin composition, and the weight ratio of the flavor protease, trypsase and pepsin is 1~3: 1~2
: 2~3；Hydrolysis temperature is 45~65 DEG C, and enzymolysis time is 2~5h, obtains enzymolysis liquid；
E, after enzymolysis terminates, the root of Dahurain angelica and perilla seed are added, extracts enzyme deactivation.The root of Dahurain angelica and perilla seed are added into the enzyme
Solve in liquid, heating and refluxing extraction, filtering, obtain mixed liquor；
F, by mixed liquor centrifugation, concentration, spray drying, the Chinese medicine composition is obtained.
According to the preparation method of described Chinese medicine composition, it is characterised in that in step D, it is described propose enzyme deactivation condition be：
Enzyme-removal temperature is 95~125 DEG C, and the enzyme deactivation time is 0.5~3h.
Preparation in accordance with the present invention, in step A, the particle diameter of the Ophidia black snake powder is 2~75 μm.The Ophidia black snake powder
Can be by the way that dry zaocys dhumnade be carried out into ultramicro grinding, then sieving obtains, such as crosses 200~600 mesh sieves.Using the grain of the present invention
The Ophidia black snake powder in footpath, enzymatic hydrolyzation can be effectively improved in the enzymolysis step in later stage, make full use of the protein ingredient in zaocys dhumnade.
The immersion is preferably soaked under agitation, so that the composition in Ophidia black snake powder is fully dissolved out.In step A, amount of water
For 3~20 times of Ophidia black snake powder weight, preferably 5~9 times.Preferably, after the completion of process is soaked, mechanical agitation is carried out, from
And make liquid uniform, it is easy to centrifuge.
In step A, the solid-liquid separating method can be filtering, centrifugation etc., and be preferably to centrifuge.In step A, leaching
Bubble process can be heated or carried out at normal temperatures, and preferably be carried out at normal temperatures.Generally, need to be heated during dipping by lye
Processing；And inventor has found, the composition in Ophidia black snake powder can be fully dissolved out by immersion at normal temperatures, so as to avoid being heated
Caused additional energy, and more convenient operation.
Preparation in accordance with the present invention, in step B, make protein denaturation using heating means.In described fire-bar
It can realize that the protein component in zaocys dhumnade is denatured completely under part, and be advantageous to fully be digested in subsequent step, together
When and avoid pollution of the chemical reagent to product.Homogeneous is carried out after heat distortion, further increases enzymolysis efficiency.
Preparation in accordance with the present invention, in step C, the suspension is adjusted to pH5~9, preferably pH7~9, more
Preferably pH7~8.In the present invention, pH is adjusted to above-mentioned alkalescent, be more beneficial for the present invention enzyme preparation digested.This
In invention, enzyme preparation is made up of flavor protease, trypsase and pepsin according to weight ratio for 1~3: 1~2: 2~3.Root
According to the preferred embodiment of the present invention, the weight ratio of flavor protease, trypsase and pepsin for 2.3~2.8: 1.3~
1.8: 2.2~2.7.According to one preferred embodiment of the invention, the weight of flavor protease, trypsase and pepsin
Than for 2.5: 1.5: 2.4.The enzymolysis process of the present invention disposably adds above-mentioned enzyme preparation, and enzyme is carried out under the conditions of above-mentioned pH
Solution, can obtain more preferably hydrolysis result.The enzyme preparation of the present invention is high for the enzymolysis efficiency of the protein component in zaocys dhumnade, bright
It is aobvious alone higher than every kind of enzyme, and it is higher than existing zaocys dhumnade active peptide enzyme.When flavor protease, trypsase and pepsin
Weight ratio be 2.5: 1.5: 2.4 when, enzymolysis efficiency highest.Preparation in accordance with the present invention, the addition of the enzyme preparation
Can be the suspension in solid content weight 0.1~2wt%, preferably 0.3~1.5wt%, more preferably 0.4~
1.2wt%.In the present invention, hydrolysis temperature is 45~65 DEG C, and enzymolysis time is 2~5h；Preferably, hydrolysis temperature is 45~60
DEG C, enzymolysis time is 2~4h.The addition of above-mentioned enzyme preparation, enzymolysis time and at a temperature of, zaocys dhumnade albumen can be realized effectively
Enzymolysis.
Preparation in accordance with the present invention, in step D, the root of Dahurain angelica and perilla seed can add the enzyme in powder form
Solve in liquid, so as to improve recovery rate.The heating and refluxing extraction time is 0.5~3 hour, preferably 1~2 hour.The filtering can
Using customary filtration methods, so as to filter off the dregs of a decoction, mixed liquor is obtained.
Preparation in accordance with the present invention, in step E, the mixed liquor is centrifuged, centrifugate is first through sheet frame pressure
Filter filters, then through ceramic membrane filter, filtrate concentration, spray drying, so as to obtain the combination of the refined active peptide containing zaocys dhumnade
Thing.Preferably, the spray-drying operation is in 80~105 DEG C of progress, so as to effectively keep the activity of zaocys dhumnade active peptide.
In the present invention, containing combination filtration adjuvant in the plate and frame filter press, the combination filtration adjuvant is by activated carbon, treasure
Zhu Yan and diatomite composition, and activated carbon, perlite and diatomaceous weight ratio 5~6: 1~2: 1~2.According to the one of the present invention
Individual preferred embodiment, it is described to combine activated carbon, perlite and diatomaceous weight ratio 5.5: 1.5: 1.5 in filtration adjuvant.
According to another preferred embodiment of the present invention, activated carbon, perlite and diatomaceous heavy in the combination filtration adjuvant
Measure ratio 5.7: 1.8: 1.3.Inventor has found that zaocys dhumnade active peptide prepared by existing method carries peculiar smell, and has bitter taste, mouthfeel
Difference；A variety of filtration adjuvants are alone to be difficult to simultaneously remove the two.The filtration adjuvant of the present invention can effectively remove zaocys dhumnade sheet
The peculiar smell and bitter taste that body carries, and after the filtration adjuvant processing by the present invention, grease-like composition is removed in enzymolysis liquid, thus
Early stage need not carry out cumbersome degreasing operation, simplify technological process.In the present invention, the aperture of the ceramic membrane can be 20~
500nm, more preferably 20~300mm.Using such ceramic membrane aperture, further refining for enzymolysis liquid can be realized.It is preferred that
Ground, the spray-drying operation is in 80~105 DEG C of progress, so as to effectively keep the activity of zaocys dhumnade active peptide.
It is pointed out that generally, the spray-drying operation of peptide is in 120~140 DEG C of progress, it is considered that such drying
Temperature is little for the activity influence of peptide.However, it is found by the inventors that the temperature of existing spray drying significantly affects the work of peptide
Property.And in the present invention, it is preferable that the spray-drying operation is in 80~105 DEG C of progress, so as to effectively keep zaocys dhumnade active peptide
Activity.
<Chinese medicine preparation>
The Chinese medicine preparation of the present invention includes above-mentioned Chinese medicine composition, can also include pharmaceutically acceptable auxiliary material.Institute
Stating the administering mode of Chinese medicine preparation can select orally.The form of Chinese drug can be selected from any in pulvis, tablet, pill
Kind.There is the Chinese medicine preparation significant dispelling wind and removing obstruction in the meridians to act on, and convenient to take.
<Purposes>
The Chinese medicine composition of the present invention can be used for preparing the medicine with dispelling wind and removing obstruction in the meridians effect, health food or common food
Product.
Embodiments of the invention are described in detail below, but protection scope of the present invention is not limited.
Embodiment 1
The parts by weight of zaocys dhumnade 80, the parts by weight of the root of Dahurain angelica 10 and the parts by weight of perilla seed 10 are mixed, add water to be heated back as solvent
Stream extraction, extraction time are 3 times, each amount of water be zaocys dhumnade, 5 times of the root of Dahurain angelica and perilla seed gross weight, each extraction time
For 2 hours, extract solution filtering, merge, concentrate, dry, obtain Chinese medicine composition A.
Embodiment 2
A, dry zaocys dhumnade is subjected to ultramicro grinding, crosses 400 mesh sieves, obtain the parts by weight of Ophidia black snake powder 77；By Ophidia black snake powder plus
Enter the water of 5 times of amounts of Ophidia black snake powder weight, mixing, adjusted with sodium hydroxide solution to pH 8, soak 13h；Mechanical agitation, centrifugation,
Take supernatant；
B, by supernatant, 25min is heated at 95 DEG C, homogeneous is then carried out, obtains suspension；
C, the suspension is adjusted into pH to 8, adds enzyme preparation and digested, obtain enzymolysis liquid；The enzyme preparation is by flavor
Protease, trypsase and pepsin are according to 2.5: 1.5: 2.4 compositions；The dosage of enzyme preparation is solid content weight in suspension
0.5wt%；Hydrolysis temperature is 50 DEG C, enzymolysis time 3h；
D, after enzymolysis terminates, the parts by weight of the root of Dahurain angelica 11 and the parts by weight of perilla seed 12 is added in the enzymolysis liquid, heated back
Stream extraction, extraction time 2h, filtering, obtains mixed liquor；
E, the mixed liquor is centrifuged, centrifugate is filtered through plate and frame filter press, and filtration adjuvant, mistake are contained in plate and frame filter press
Filter auxiliary agent is made up of activated carbon, perlite and diatomite according to weight ratio 5.7: 1.8: 1.3；Via hole diameter is 400nm ceramics again
Membrane filtration, filtrate concentration, in 85 DEG C of spray drying in middle low-temperature spray drying tower, obtain the Chinese medicine group of the active peptide containing zaocys dhumnade
Compound.
Embodiment 3
By the parts by weight of Chinese medicine composition 82, the parts by weight of lactose 16, the parts by weight of microcrystalline cellulose 1, tristearin made from embodiment 1
The sour parts by weight of magnesium 1, sieving, weigh, mix, pelletizing, tabletting.
Embodiment 4
By parts by weight of Chinese medicine composition 82 made from the embodiment 1, parts by weight of PURE WHEY 8, the parts by weight of konjaku flour 5, anti-
Property dextrin 2 parts by weight, the parts by weight of crystal diabetin 2, the parts by weight of inulin 1, by sieving, weighing, mixing, being packaged to be pulvis.
Comparative example 1
Except the acetum of step B in embodiment 1 is replaced with into hydrochloric acid solution, other steps are identical with documents 1.
Comparative example 2
Except the spray drying temperature of step E in embodiment 1 is arranged into 135 DEG C, other steps are same as Example 1.
Comparative example 3
Activated carbon except the filtration adjuvant of step E in embodiment 2 to be replaced with to equivalent weight, other steps and embodiment
2 is identical.
Comparative example 4
Perlite except the filtration adjuvant of step E in embodiment 2 to be replaced with to equivalent weight, other steps and embodiment
2 is identical.
Comparative example 5
Diatomite except the filtration adjuvant of step E in embodiment 2 to be replaced with to equivalent weight, other steps and embodiment
2 is identical.
Bitter taste and fishy smell in embodiment 2 and comparative example 1~5 are determined, the results are shown in Table 1.
Bitter taste estimate method is：Take 1g zaocys dhumnades active peptide to add in 10ml warm water to stir, extension rate is calculated as
Bitterness value, calculate the average bitterness value of 20 people.
Fishy smell evaluation method is：Evaluation method is：0- is without fishy smell；1- slightly has fishy smell；2- fishy smell is weaker；3- has fishy smell；4-
Fishy smell is general；5- fishy smell is laid particular stress on；6- fishy smell is heavier；The average fishy smell value of the 7- fishy smell very people of re-computation 20.
Table 1
The present invention is not limited to above-mentioned embodiment, in the case of without departing substantially from the substantive content of the present invention, this area skill
Any deformation, improvement, the replacement that art personnel are contemplated that each fall within the scope of the present invention.
Claims (10)
1. a kind of Chinese medicine composition of the peptide containing zaocys dhumnade with dispelling wind and removing obstruction in the meridians effect, it is characterised in that the composition is by such as
Lower bulk drug is made：
Zaocys dhumnade 60-90 parts by weight,
Root of Dahurain angelica 5-30 parts by weight, and
Perilla seed 5-30 parts by weight.
2. Chinese medicine composition according to claim 1, it is characterised in that the composition is made up of the following raw material medicine：
Zaocys dhumnade 70-80 parts by weight,
Root of Dahurain angelica 10-20 parts by weight, and
Perilla seed 10-20 parts by weight.
3. Chinese medicine composition according to claim 1, it is characterised in that the composition is made up of the following raw material medicine：
The parts by weight of zaocys dhumnade 80, the parts by weight of the root of Dahurain angelica 10, and the parts by weight of perilla seed 10；Or
The parts by weight of zaocys dhumnade 75, the parts by weight of the root of Dahurain angelica 13, and the parts by weight of perilla seed 12；Or
The parts by weight of zaocys dhumnade 77, the parts by weight of the root of Dahurain angelica 11, and the parts by weight of perilla seed 12.
4. the preparation method of the Chinese medicine composition according to claim any one of 1-3, it is characterised in that by the black tip
Snake, the root of Dahurain angelica and perilla seed powder the concentration of gained extract solution, are dried by the use of water as solvent extraction, obtain the Chinese medicine composition.
5. the preparation method of the Chinese medicine composition according to claim any one of 1-3, it is characterised in that it includes following step
Suddenly：
A, zaocys dhumnade is crushed, obtains Ophidia black snake powder；The Ophidia black snake powder is added into water, adjusts pH to 8~9, immersion 12~15
Hour, separation of solid and liquid, take supernatant；
B, supernatant regulation pH to 4~6 is precipitated, separation of solid and liquid, takes solid portion；
C, the solid portion is added into water, 20~40min is heated at 85~100 DEG C, homogeneous is then carried out, is suspended
Liquid；
D, the suspension is adjusted into pH to 5~9, adds enzyme preparation and digested；The enzyme preparation is by flavor protease, pancreas egg
White enzyme and pepsin composition, and the weight ratio of the flavor protease, trypsase and pepsin be 1~3: 1~2: 2~
3；Hydrolysis temperature is 45~65 DEG C, and enzymolysis time is 2~5h, obtains enzymolysis liquid；
E, after enzymolysis terminates, the root of Dahurain angelica and perilla seed are added, extracts enzyme deactivation.The root of Dahurain angelica and perilla seed are added into the enzymolysis liquid
In, heating and refluxing extraction, filtering, obtain mixed liquor；
F, by mixed liquor centrifugation, concentration, spray drying, the Chinese medicine composition is obtained.
6. the preparation method of Chinese medicine composition according to claim 5, it is characterised in that in step B, using weak acid by institute
State supernatant regulation pH to 4~6；The weak acid is selected from least one of acetic acid, lactic acid, citric acid.
7. the preparation method of Chinese medicine composition according to claim 5, it is characterised in that in step E, the extraction enzyme deactivation
Condition be：Enzyme-removal temperature is 95~125 DEG C, and the enzyme deactivation time is 0.5~3h.
8. the preparation method of Chinese medicine composition according to claim 5, it is characterised in that in step E, at the enzyme deactivation
Enzymolysis liquid after reason is centrifuged, and centrifugate filters through plate and frame filter press first, then through ceramic membrane filter, is obtained containing active peptide
Permeate；The permeate containing active peptide is concentrated, spray drying, so as to obtain the Chinese medicine composition；The spraying is dry
It is dry to be carried out at 80~105 DEG C；Containing combination filtration adjuvant in the plate and frame filter press, the combination filtration adjuvant is by activity
Charcoal, perlite and diatomite form according to weight ratio 5~6: 1~2: 1~2.
9. a kind of Chinese medicine preparation with dispelling wind and removing obstruction in the meridians effect, it is characterised in that the Chinese medicine preparation includes claims 1 to 3
Chinese medicine composition made from Chinese medicine composition described in any one or the preparation method according to claim any one of 4-8.
10. the Chinese medicine composition according to any one of claims 1 to 3 or the system according to any one of claim 4~8
Chinese medicine composition made from Preparation Method is used to prepare the purposes of medicine or health food with dispelling wind and removing obstruction in the meridians effect.
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| CN201711068502.8A CN107693575A (en) | 2017-11-02 | 2017-11-02 | Chinese medicine composition of the peptide containing zaocys dhumnade with dispelling wind and removing obstruction in the meridians effect and its production and use |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711068502.8A CN107693575A (en) | 2017-11-02 | 2017-11-02 | Chinese medicine composition of the peptide containing zaocys dhumnade with dispelling wind and removing obstruction in the meridians effect and its production and use |
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| CN107693575A true CN107693575A (en) | 2018-02-16 |
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|---|---|---|---|---|
| CN101701240A (en) * | 2009-11-12 | 2010-05-05 | 常州康和生物技术有限公司 | Method for preparing antihypertensive peptide by utilizing combined enzyme enzymolysis silkworm chrysalis protein |
| CN101748180A (en) * | 2010-01-18 | 2010-06-23 | 山东世纪春食品有限公司 | Functional peanut small molecule mixed polypeptide, preparation method and application thereof |
| CN102286586A (en) * | 2011-07-29 | 2011-12-21 | 唐作安 | Method for preparing oligopeptide of snake meat |
| CN102302161A (en) * | 2011-09-02 | 2012-01-04 | 唐作安 | Preparation method of ophidine oral liquid |
| CN104789629A (en) * | 2015-04-30 | 2015-07-22 | 中国食品发酵工业研究院 | Chickpea oligopeptide and preparation method thereof |
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2017
- 2017-11-02 CN CN201711068502.8A patent/CN107693575A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101701240A (en) * | 2009-11-12 | 2010-05-05 | 常州康和生物技术有限公司 | Method for preparing antihypertensive peptide by utilizing combined enzyme enzymolysis silkworm chrysalis protein |
| CN101748180A (en) * | 2010-01-18 | 2010-06-23 | 山东世纪春食品有限公司 | Functional peanut small molecule mixed polypeptide, preparation method and application thereof |
| CN102286586A (en) * | 2011-07-29 | 2011-12-21 | 唐作安 | Method for preparing oligopeptide of snake meat |
| CN102302161A (en) * | 2011-09-02 | 2012-01-04 | 唐作安 | Preparation method of ophidine oral liquid |
| CN104789629A (en) * | 2015-04-30 | 2015-07-22 | 中国食品发酵工业研究院 | Chickpea oligopeptide and preparation method thereof |
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