CN107669849B - An antiallergic agent prepared from coconut shell extract and rutin - Google Patents
An antiallergic agent prepared from coconut shell extract and rutin Download PDFInfo
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- CN107669849B CN107669849B CN201710999298.5A CN201710999298A CN107669849B CN 107669849 B CN107669849 B CN 107669849B CN 201710999298 A CN201710999298 A CN 201710999298A CN 107669849 B CN107669849 B CN 107669849B
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- rutin
- coconut shell
- extract
- coconut
- shell extract
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- 244000060011 Cocos nucifera Species 0.000 title claims abstract description 55
- 235000013162 Cocos nucifera Nutrition 0.000 title claims abstract description 55
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 title claims abstract description 25
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 title claims abstract description 25
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 title claims abstract description 25
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 title claims abstract description 25
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 235000005493 rutin Nutrition 0.000 title claims abstract description 25
- 229960004555 rutoside Drugs 0.000 title claims abstract description 25
- 239000000043 antiallergic agent Substances 0.000 title claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000011347 resin Substances 0.000 claims abstract description 9
- 229920005989 resin Polymers 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 6
- 239000003480 eluent Substances 0.000 claims abstract description 6
- 239000000706 filtrate Substances 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 238000010992 reflux Methods 0.000 claims abstract description 6
- 238000000605 extraction Methods 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 230000003266 anti-allergic effect Effects 0.000 abstract description 7
- 230000002708 enhancing effect Effects 0.000 abstract description 7
- 108010003272 Hyaluronate lyase Proteins 0.000 abstract description 6
- 102000001974 Hyaluronidases Human genes 0.000 abstract description 6
- 229960002773 hyaluronidase Drugs 0.000 abstract description 6
- 239000002537 cosmetic Substances 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 5
- 230000005764 inhibitory process Effects 0.000 abstract description 5
- 229940079593 drug Drugs 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 241000233788 Arecaceae Species 0.000 description 1
- 241000737241 Cocos Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 235000020415 coconut juice Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000037072 sun protection Effects 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/889—Arecaceae, Palmae or Palmaceae (Palm family), e.g. date or coconut palm or palmetto
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Birds (AREA)
- Botany (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Dermatology (AREA)
- Molecular Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Cosmetics (AREA)
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Abstract
The invention relates to a coconut shell extract and application thereof in enhancing rutin antiallergic capability, and a preparation method of the coconut shell extract comprises the following steps: (1) taking dried coconut shells, crushing the dried coconut shells to 20-100 meshes, adding 80% ethanol with the volume fraction of 8-15 times of the mass of the coconut shells, performing reflux extraction for 6-10 hours, filtering while hot, removing filter residues, and performing reduced pressure concentration on the filtrate to obtain an extract; (2) and (2) adsorbing the extract obtained in the step (1) by using a macroporous resin column, eluting by using 60-70% ethanol in volume fraction, collecting eluent with the volume of 4-6 times that of the column, concentrating under reduced pressure, and drying in vacuum to obtain the coconut shell extract. The coconut shell extract has the effect of enhancing the inhibition rate of rutin on hyaluronidase, namely enhancing the anti-allergic capability of rutin, can be developed and used for preparing an anti-allergic agent, and can be used in the fields of medicines, cosmetics and the like.
Description
Technical Field
The invention belongs to the field of whitening and sun protection, and particularly relates to a coconut shell extract and application thereof in enhancing rutin antiallergic capability.
Background
Coconut (coconut nucifera L.) is a monocotyledonous plant of the genus Cocos belonging to the family Palmae, and is a very valuable crop of tropical economy. Coconut meat is edible or can be used for expelling oil, and coconut water (endosperm) can be used as beverage. Coconut shell (i.e. coconut endocarp) and coconut chaff are byproducts of deep processing of coconut, the coconut shell is black brown and firm, at present, the coconut shell is mainly used for preparing coconut shell carbon through carbonization and further preparing activated carbon, and no report is provided for other applications. The invention adopts organic solvent to extract the coconut shells, and finds that the extract has the capability of enhancing rutin to clear free radicals, so that the coconut shell extract can be developed and used for preparing antioxidants to be used in the fields of medicines, foods, cosmetics and the like.
Disclosure of Invention
The invention provides a coconut shell extract, which is characterized in that the preparation method of the coconut shell extract comprises the following steps:
(1) taking dried coconut shells, crushing the dried coconut shells to 20-100 meshes, adding 80% ethanol with the volume fraction of 8-15 times of the mass of the coconut shells, performing reflux extraction for 6-10 hours, filtering while hot, removing filter residues, and performing reduced pressure concentration on the filtrate to obtain an extract;
(2) and (2) adsorbing the extract obtained in the step (1) by using a macroporous resin column, eluting by using 60-70% ethanol in volume fraction, collecting eluent with the volume of 4-6 times that of the column, concentrating under reduced pressure, and drying in vacuum to obtain the coconut shell extract.
The macroporous resin in the step (2) is selected from D101 type or AB8 type.
Another embodiment of the present invention provides a method for preparing the coconut shell extract, which comprises the following steps:
(1) taking dried coconut shells, crushing the dried coconut shells to 20-100 meshes, adding 80% ethanol with the volume fraction of 8-15 times of the mass of the coconut shells, performing reflux extraction for 6-10 hours, filtering while hot, removing filter residues, and performing reduced pressure concentration on the filtrate to obtain an extract;
(2) and (2) adsorbing the extract obtained in the step (1) by using a macroporous resin column, eluting by using 60-70% ethanol in volume fraction, collecting eluent with the volume of 4-6 times that of the column, concentrating under reduced pressure, and drying in vacuum to obtain the coconut shell extract.
The macroporous resin in the step (2) is selected from D101 type or AB8 type.
Another embodiment of the present invention provides the use of the coconut shell extract as described above for the preparation of an antiallergic agent. The antiallergic agent can be used in medicine, cosmetics, etc.
Another embodiment of the invention provides the application of the coconut shell extract in enhancing rutin antiallergic effect.
An antiallergic agent characterized by comprising the coconut shell extract of the present invention.
An antiallergic agent is characterized by comprising the coconut shell extract and rutin.
An antiallergic agent is characterized by comprising the coconut shell extract and rutin, wherein the amount of the coconut shell extract is 6-8% of the weight of the rutin.
Compared with the prior art, the invention has the advantages that: the invention develops another new application of coconut shells which are byproducts of deep processing of coconuts, namely, the coconut shell extract can enhance the inhibition rate of rutin on hyaluronidase, namely enhance the anti-allergic capability of rutin, can be developed and used for preparing an anti-allergic agent, is used in the fields of medicines, cosmetics and the like, and greatly enriches the application of coconut shell wastes.
Detailed Description
In order to facilitate a further understanding of the invention, the following examples are provided to illustrate it in more detail. However, these examples are only for better understanding of the present invention and are not intended to limit the scope or the principle of the present invention, and the embodiments of the present invention are not limited to the following.
Example 1
(1) Pulverizing dried coconut shell (1.0kg) to 100 mesh, adding 8.0kg ethanol solution with volume fraction of 80%, reflux extracting for 6 hr, filtering while hot, removing filter residue, and concentrating the filtrate under reduced pressure to obtain extract;
(2) and (2) adsorbing the extract obtained in the step (1) by using a macroporous resin column, eluting by using 60-70% ethanol in volume fraction, collecting eluent with the volume of 4-6 times that of the column, concentrating under reduced pressure, and drying in vacuum to obtain a coconut shell extract (16.8 g, hereinafter referred to as a product A).
Example 2
(1) Pulverizing dried coconut shell (1.0kg) to 20 mesh, adding 15.0kg ethanol solution with volume fraction of 80%, reflux extracting for 10 hr, hot filtering, removing residue, and concentrating the filtrate under reduced pressure to obtain extract;
(2) and (2) adsorbing the extract obtained in the step (1) by using a macroporous resin column, eluting by using 60-70% ethanol in volume fraction, collecting eluent with the volume of 4-6 times that of the column, concentrating under reduced pressure, and drying in vacuum to obtain a coconut shell extract (19.5 g, hereinafter referred to as a product B).
Example 3 anti-allergic test
Samples tested at concentrations of 30. mu.g/mL and 60. mu.g/mL, respectively, were: rutin, a product A, a product B, a product A and rutin (the mass of the product A is 6 percent of the mass of the rutin), and a product B and rutin (the mass of the product B is 8 percent of the mass of the rutin) have the inhibition rate on hyaluronidase, and the following test results are according to the Chinese patent application numbers: 201310531168.0, see table 1.
TABLE 1
"-" indicates that the sample inhibited hyaluronidase by less than 10%.
The experimental results show that the product A, B has almost no inhibition effect on hyaluronidase, but when the product is mixed with rutin, particularly when the amount of the rutin is 6-8% of the weight of the rutin, the inhibition of the rutin on the hyaluronidase can be obviously enhanced; the experimental result shows that the coconut shell extract can be used for enhancing the antiallergic effect of rutin, and further used as an additive for preparing an antiallergic agent in the fields of medicines, cosmetics and the like.
Claims (1)
1. An antiallergic agent is characterized in that the antiallergic agent is prepared from coconut shell extract and rutin, wherein the amount of the coconut shell extract is 6-8% of the weight of the rutin; the preparation method of the coconut shell extract comprises the following steps: (1) taking dried coconut shells, crushing the dried coconut shells to 20-100 meshes, adding 80% ethanol with the volume fraction of 8-15 times of the mass of the coconut shells, performing reflux extraction for 6-10 hours, filtering while hot, removing filter residues, and performing reduced pressure concentration on the filtrate to obtain an extract; (2) adsorbing the extract obtained in the step (1) by using a macroporous resin column, eluting by using 60-70% ethanol in volume fraction, collecting eluent with the volume 4-6 times that of the column, concentrating under reduced pressure, and drying in vacuum to obtain the coconut shell extract; the macroporous resin in the step (2) is selected from D101 type or AB8 type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710999298.5A CN107669849B (en) | 2017-10-23 | 2017-10-23 | An antiallergic agent prepared from coconut shell extract and rutin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710999298.5A CN107669849B (en) | 2017-10-23 | 2017-10-23 | An antiallergic agent prepared from coconut shell extract and rutin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107669849A CN107669849A (en) | 2018-02-09 |
| CN107669849B true CN107669849B (en) | 2021-02-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710999298.5A Expired - Fee Related CN107669849B (en) | 2017-10-23 | 2017-10-23 | An antiallergic agent prepared from coconut shell extract and rutin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107669849B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107596047A (en) * | 2017-10-23 | 2018-01-19 | 深圳百奥捷生物科技有限公司 | A kind of coconut husk extract and its application in terms of rutin oxidation resistance is strengthened |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773589A (en) * | 2010-01-12 | 2010-07-14 | 海南大学 | Production method of coconut polyphenol extracts |
| CN107596047A (en) * | 2017-10-23 | 2018-01-19 | 深圳百奥捷生物科技有限公司 | A kind of coconut husk extract and its application in terms of rutin oxidation resistance is strengthened |
-
2017
- 2017-10-23 CN CN201710999298.5A patent/CN107669849B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773589A (en) * | 2010-01-12 | 2010-07-14 | 海南大学 | Production method of coconut polyphenol extracts |
| CN107596047A (en) * | 2017-10-23 | 2018-01-19 | 深圳百奥捷生物科技有限公司 | A kind of coconut husk extract and its application in terms of rutin oxidation resistance is strengthened |
Non-Patent Citations (1)
| Title |
|---|
| "Ethanolic coconut husk extract: In vitro antioxidative activity and effect on oxidative stability of shrimp oil emulsion;Natchaphol Buamard and Soottawat Benjakul;《European journal of lipid science and technology》;20170726;1-11页 * |
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| Publication number | Publication date |
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| CN107669849A (en) | 2018-02-09 |
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