CN107596047A - A kind of coconut husk extract and its application in terms of rutin oxidation resistance is strengthened - Google Patents
A kind of coconut husk extract and its application in terms of rutin oxidation resistance is strengthened Download PDFInfo
- Publication number
- CN107596047A CN107596047A CN201711004848.1A CN201711004848A CN107596047A CN 107596047 A CN107596047 A CN 107596047A CN 201711004848 A CN201711004848 A CN 201711004848A CN 107596047 A CN107596047 A CN 107596047A
- Authority
- CN
- China
- Prior art keywords
- coconut husk
- extract
- husk extract
- rutin
- volume fraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Cosmetics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
A kind of application the present invention relates to coconut husk extract and its in terms of rutin oxidation resistance is strengthened, the preparation method of coconut husk extract of the present invention comprise the following steps：(1) dry coconut husk is taken, after being crushed to 20 100 mesh, the volume fraction for adding 5 to 10 times of amounts of its quality is 80% ethanol, and refluxing extraction filters while hot after 6 to 10 hours, removes filter residue, medicinal extract is obtained after filtrate decompression concentration；(2) medicinal extract that step (1) obtains adsorbs through macroporous resin column, with the ethanol elution that volume fraction is 60 70%, collects the eluent of 46 times of amount column volumes, is concentrated under reduced pressure, is dried in vacuo to obtain the coconut husk extract.Coconut husk extract of the present invention has enhancing rutin to hydroxyl radical free radical and the Scavenging activity of DPPH free radicals, can be exploited for preparing antioxidant, for fields such as medicine, food, cosmetics.
Description
Technical field
The invention belongs to whitening sun protection field, and in particular to a kind of coconut husk extract and its enhancing rutin oxidation resistance
The application of aspect.
Background technology
Coconut (Cocos nucifera L.) is the monocotyledon of Palmae cocoanut, is that one kind has Development volue very much
Tropical industrial crops.Coconut meat is edible, can also extract oil, and coconut water (endosperm) can be beverage etc..Coconut husk (i.e. coconut endocarp), coconut palm
Chaff is the byproduct of coconut deep processing, and coconut husk is in dark brown, and quality is very solid, and at present, mainly application is made through charing to coconut husk
Into coconut husk charcoal, activated carbon is further made, otherwise application there is no report.The present invention is carried out using organic solvent to coconut husk
Extraction, the ability that there is its extract enhancing rutin to understand free radical is found, therefore, coconut husk extract can be exploited for preparing
Antioxidant is used for the fields such as medicine, food, cosmetics.
The content of the invention
The present invention provides a kind of coconut husk extract, it is characterised in that the preparation method of the coconut husk extract includes following step
Suddenly：
(1) dry coconut husk is taken, after being crushed to 20-100 mesh, the volume fraction for adding 5 to 10 times of amounts of its quality is
80% ethanol, refluxing extraction filter while hot after 6 to 10 hours, remove filter residue, medicinal extract is obtained after filtrate decompression concentration；
(2) medicinal extract that step (1) obtains adsorbs through macroporous resin column, the ethanol elution for being 60-70% with volume fraction, receives
Collect the 4-6 times of eluent for measuring column volume, be concentrated under reduced pressure, be dried in vacuo to obtain the coconut husk extract.
Macroreticular resin described in step (2) is selected from D101 types or AB8 types.
Another embodiment of the present invention provides the preparation method of above-mentioned coconut husk extract, it is characterised in that including following step
Suddenly：
(1) dry coconut husk is taken, after being crushed to 20-100 mesh, the volume fraction for adding 5 to 10 times of amounts of its quality is
80% ethanol, refluxing extraction filter while hot after 6 to 10 hours, remove filter residue, medicinal extract is obtained after filtrate decompression concentration；
(2) medicinal extract that step (1) obtains adsorbs through macroporous resin column, the ethanol elution for being 60-70% with volume fraction, receives
Collect the 4-6 times of eluent for measuring column volume, be concentrated under reduced pressure, be dried in vacuo to obtain the coconut husk extract.
Macroreticular resin described in step (2) is selected from D101 types or AB8 types.
Another embodiment of the present invention provides application of the above-mentioned coconut husk extract in antioxidant is prepared.The antioxidant can
Applied to fields such as medicine, food, cosmetics.
Another embodiment of the present invention provides application of the above-mentioned coconut husk extract in enhancing rutin removes free radical.
A kind of antioxidant, it is characterised in that including coconut husk extract of the present invention.
A kind of antioxidant, it is characterised in that including coconut husk extract of the present invention and rutin.
A kind of antioxidant, it is characterised in that including coconut husk extract of the present invention and rutin, the two mass ratio is
1：1.
Compared with prior art, the advantage of the invention is that：The present invention develops the byproduct of coconut deep processing --- coconut palm
Another new application of shell, i.e. coconut husk extract can strengthen rutin to hydroxyl radical free radical and the Scavenging activity of DPPH free radicals, can quilt
Develop for preparing antioxidant, for fields such as medicine, food, cosmetics, be greatly enriched the purposes of coconut husk discarded object.
Embodiment
For the ease of a further understanding of the present invention, examples provided below has done more detailed description to it.But
It is that these embodiments only are not used for limiting the scope of the present invention or implementation principle, reality of the invention for being better understood from inventing
The mode of applying is not limited to herein below.
Embodiment 1
(1) take dry coconut husk (1.0kg) to be crushed to 100 mesh, add the ethanol solution that 5.0kg volume fractions are 80%,
After refluxing extraction 6 hours, filter while hot, remove filter residue, medicinal extract is obtained after filtrate decompression concentration；
(2) medicinal extract that step (1) obtains adsorbs through macroporous resin column, the ethanol elution for being 60-70% with volume fraction, receives
Collect the 4-6 times of eluent for measuring column volume, be concentrated under reduced pressure, be dried in vacuo to obtain coconut husk extract (15.6g, hereinafter referred to as product A).
Embodiment 2
(1) take dry coconut husk (1.0kg) to be crushed to 20 mesh, add the ethanol solution that 10.0kg volume fractions are 80%,
After refluxing extraction 10 hours, filter while hot, remove filter residue, medicinal extract is obtained after filtrate decompression concentration；
(2) medicinal extract that step (1) obtains adsorbs through macroporous resin column, the ethanol elution for being 60-70% with volume fraction, receives
Collect the 4-6 times of eluent for measuring column volume, be concentrated under reduced pressure, be dried in vacuo to obtain coconut husk extract (18.4g, hereinafter referred to as product B).
The radical scavenging activity of the test sample of embodiment 3
According to the method for prior art, testing rutin, product A, product B, product A+ rutins respectively, (the two mass ratio is 1：
1), (the two mass ratio is 1 to product B+ rutins：1) to hydroxyl radical free radical and the Scavenging activity of DPPH free radicals, it is dense to calculate semi-inhibit
Spend (IC50Value), the following test result of the present invention is according to Chinese Patent Application No.：201310089721.X method described in is carried out
Test, it is shown in Table 1.
Table 1
" +++ " expression compound concentration is 0.1-1.0 μ g/mL, and " ++ " expression compound concentration is 10-100 μ g/mL, "-"
It is 1 × 10 to represent that compound concentration is more than5μg/mL。
Above-mentioned experimental result shows that product A, B almost do not have radical scavenging activity in itself, but works as it with rutin with matter
Measure ratio 1：During 1 mixing, Scavenging activity of the rutin to free radical can be significantly increased；This test result indicates that, coconut husk extract can
For strengthening the antioxidant effect of rutin, and then as additive for preparing antioxidant, for medicine, food, cosmetics etc.
Field.
Claims (8)
1. a kind of coconut husk extract, it is characterised in that the preparation method of the coconut husk extract comprises the following steps：
(1) dry coconut husk is taken, after being crushed to 20-100 mesh, the volume fraction for adding 5 to 10 times of amounts of its quality is 80%
Ethanol, refluxing extraction filters while hot after 6 to 10 hours, removes filter residue, after filtrate decompression concentration medicinal extract；
(2) medicinal extract that step (1) obtains adsorbs through macroporous resin column, the ethanol elution for being 60-70% with volume fraction, collects 4-
The eluent of 6 times of amount column volumes, is concentrated under reduced pressure, is dried in vacuo to obtain the coconut husk extract.
2. the coconut husk extract described in claim 1, it is characterised in that macroreticular resin described in step (2) be selected from D101 types or
AB8 types.
3. the preparation method of the coconut husk extract described in claim any one of 1-2, it is characterised in that comprise the following steps：
(1) dry coconut husk is taken, after being crushed to 20-100 mesh, the volume fraction for adding 5 to 10 times of amounts of its quality is 80%
Ethanol, refluxing extraction filters while hot after 6 to 10 hours, removes filter residue, after filtrate decompression concentration medicinal extract；
(2) medicinal extract that step (1) obtains adsorbs through macroporous resin column, the ethanol elution for being 60-70% with volume fraction, collects 4-
The eluent of 6 times of amount column volumes, is concentrated under reduced pressure, is dried in vacuo to obtain the coconut husk extract.
4. the preparation method described in claim 3, it is characterised in that the macroreticular resin described in step (2) is selected from D101 types or AB8
Type.
5. application of the coconut husk extract described in claim any one of 1-2 in antioxidant is prepared.
6. application of the coconut husk extract in enhancing rutin removes free radical described in claim any one of 1-2.
7. a kind of antioxidant, it is characterised in that including the coconut husk extract described in claim any one of 1-2.
8. the antioxidant described in claim 7, it is characterised in that also including rutin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711004848.1A CN107596047A (en) | 2017-10-23 | 2017-10-23 | A kind of coconut husk extract and its application in terms of rutin oxidation resistance is strengthened |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711004848.1A CN107596047A (en) | 2017-10-23 | 2017-10-23 | A kind of coconut husk extract and its application in terms of rutin oxidation resistance is strengthened |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107596047A true CN107596047A (en) | 2018-01-19 |
Family
ID=61079915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711004848.1A Pending CN107596047A (en) | 2017-10-23 | 2017-10-23 | A kind of coconut husk extract and its application in terms of rutin oxidation resistance is strengthened |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107596047A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107669849A (en) * | 2017-10-23 | 2018-02-09 | 深圳百奥捷生物科技有限公司 | A kind of coconut husk extract and its application in terms of rutin antiallergy ability is strengthened |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773589A (en) * | 2010-01-12 | 2010-07-14 | 海南大学 | Production method of coconut polyphenol extracts |
| CN107669849A (en) * | 2017-10-23 | 2018-02-09 | 深圳百奥捷生物科技有限公司 | A kind of coconut husk extract and its application in terms of rutin antiallergy ability is strengthened |
-
2017
- 2017-10-23 CN CN201711004848.1A patent/CN107596047A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773589A (en) * | 2010-01-12 | 2010-07-14 | 海南大学 | Production method of coconut polyphenol extracts |
| CN107669849A (en) * | 2017-10-23 | 2018-02-09 | 深圳百奥捷生物科技有限公司 | A kind of coconut husk extract and its application in terms of rutin antiallergy ability is strengthened |
Non-Patent Citations (3)
| Title |
|---|
| NATCHAPHOL BUAMARD AND SOOTTAWAT BENJAKUL: "Ethanolic coconut husk extract: In vitro antioxidative activity and effect on oxidative stability of shrimp oil emulsion", 《EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY》 * |
| 倪少凯: "海南高种椰壳醇提取物抗11种常见真菌的实验研究", 《广东医学院学报》 * |
| 王振宇: "《生物活性成分分离技术》", 31 May 2015, 哈尔滨工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107669849A (en) * | 2017-10-23 | 2018-02-09 | 深圳百奥捷生物科技有限公司 | A kind of coconut husk extract and its application in terms of rutin antiallergy ability is strengthened |
| CN107669849B (en) * | 2017-10-23 | 2021-02-05 | 深圳百奥捷生物科技有限公司 | An antiallergic agent prepared from coconut shell extract and rutin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107550822A (en) | A kind of coconut palm chaff extract and its application in collaboration is anti-oxidant | |
| CN101618114A (en) | Method for preparing total glycollic glucoside of royal paulownia (original variety) fortune paulownia wood applications of same to foods and medicines | |
| CN101766690B (en) | Multipurpose utilization method for improving the value of schisandra chinensis medicinal material | |
| CN109293572A (en) | A kind of method of erythrothioneine and polysaccharide in extraction Pleurotus citrinopileatus | |
| CN103860620A (en) | Antioxidant active extract of snow chrysanthemum and preparation method thereof | |
| CN107596047A (en) | A kind of coconut husk extract and its application in terms of rutin oxidation resistance is strengthened | |
| CN104262446A (en) | Method of extracting momordica glycoside V from momordica grosvenori | |
| CN107669849A (en) | A kind of coconut husk extract and its application in terms of rutin antiallergy ability is strengthened | |
| CN106496007B (en) | The separating and extracting process of 6-gingerol in ginger | |
| CN105777841B (en) | A kind of method for removing Propamocarb in Araliaceae saponin extract | |
| CN107693652A (en) | A kind of coconut palm chaff extract and its application in antiallergy is cooperateed with | |
| CN102180985A (en) | Method for extracting and purifying mugwort polysaccharides | |
| CN104817601B (en) | Three flavones ingredient mixtures, preparation and its method are extracted from Herba lysimachiae capillipedis | |
| JP3665298B2 (en) | Extraction method of triterpene | |
| CN107006837A (en) | A kind of mulberry leaf cyclodextrin antioxidant and preparation method thereof | |
| CN104523881A (en) | Method for extracting and purifying triterpenoids from apple pomace and application of triterpenoids | |
| CN110776541B (en) | Preparation method and application of quercetin-3-gentiobioside | |
| CN107692230A (en) | Health care product containing resveratrol and luteolin and preparation method and application thereof | |
| CN105732839A (en) | Preparation method of high-activity tricholoma matsutake polysaccharide | |
| JP2009225698A (en) | Method for utilizing isothiocyanate-containing plant | |
| CN107198668B (en) | Extract of antioxidant components in camellia seed hulls as well as extraction method and application thereof | |
| Kumar et al. | Green and proficient process for industrial-scale preparation of Gymnema sylvestre standardized-extract enriched with Gymnemic acids through polymer-matrix-adsorption to reduce hyperglycemia | |
| CN103054919B (en) | Preparation method of macassar nutmeg lignan extract | |
| CN108186470A (en) | A kind of compound Chinese medicine extract and its application as whitening sunscreen agent | |
| CN105030891B (en) | preparation method and application of dracocephalum heterophyllum flavonoid component |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180119 |
|
| RJ01 | Rejection of invention patent application after publication |