CN107692230A - Health products containing resveratrol and cyanidenon and its preparation method and application - Google Patents
Health products containing resveratrol and cyanidenon and its preparation method and application Download PDFInfo
- Publication number
- CN107692230A CN107692230A CN201710946012.7A CN201710946012A CN107692230A CN 107692230 A CN107692230 A CN 107692230A CN 201710946012 A CN201710946012 A CN 201710946012A CN 107692230 A CN107692230 A CN 107692230A
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- CN
- China
- Prior art keywords
- resveratrol
- cyanidenon
- extract
- health products
- extraction
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- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 title claims abstract description 115
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 title claims abstract description 112
- 235000021283 resveratrol Nutrition 0.000 title claims abstract description 112
- 229940016667 resveratrol Drugs 0.000 title claims abstract description 112
- 230000036541 health Effects 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000000284 extract Substances 0.000 claims abstract description 98
- 239000000203 mixture Substances 0.000 claims abstract description 28
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 21
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 20
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims abstract description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 7
- 240000000249 Morus alba Species 0.000 claims abstract description 6
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 62
- 244000105624 Arachis hypogaea Species 0.000 claims description 57
- 235000020232 peanut Nutrition 0.000 claims description 57
- 238000000605 extraction Methods 0.000 claims description 56
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 44
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 44
- 235000018262 Arachis monticola Nutrition 0.000 claims description 44
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 39
- 239000003480 eluent Substances 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 23
- 235000013305 food Nutrition 0.000 claims description 20
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 16
- 238000011084 recovery Methods 0.000 claims description 13
- 238000000746 purification Methods 0.000 claims description 10
- 239000011347 resin Substances 0.000 claims description 9
- 229920005989 resin Polymers 0.000 claims description 9
- 238000001179 sorption measurement Methods 0.000 claims description 7
- 239000003463 adsorbent Substances 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 239000003208 petroleum Substances 0.000 claims description 6
- 238000002390 rotary evaporation Methods 0.000 claims description 6
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 241001128140 Reseda Species 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000003610 charcoal Substances 0.000 claims description 4
- 239000000945 filler Substances 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 235000018991 trans-resveratrol Nutrition 0.000 claims description 3
- 244000025254 Cannabis sativa Species 0.000 claims description 2
- 241000282806 Rhinoceros Species 0.000 claims description 2
- 239000002023 wood Substances 0.000 claims description 2
- 241000489523 Veratrum Species 0.000 claims 2
- 229930182470 glycoside Natural products 0.000 claims 2
- 150000002338 glycosides Chemical class 0.000 claims 2
- 238000007873 sieving Methods 0.000 claims 1
- 229960001866 silicon dioxide Drugs 0.000 claims 1
- 239000003205 fragrance Substances 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 32
- 238000004128 high performance liquid chromatography Methods 0.000 description 18
- 238000000034 method Methods 0.000 description 14
- 230000000694 effects Effects 0.000 description 10
- 238000010828 elution Methods 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000001093 anti-cancer Effects 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 5
- 206010061218 Inflammation Diseases 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 235000011187 glycerol Nutrition 0.000 description 4
- 230000004054 inflammatory process Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000008595 infiltration Effects 0.000 description 3
- 238000001764 infiltration Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 239000004575 stone Substances 0.000 description 3
- 238000004809 thin layer chromatography Methods 0.000 description 3
- OEGPRYNGFWGMMV-UHFFFAOYSA-N (3,4-dimethoxyphenyl)methanol Chemical compound COC1=CC=C(CO)C=C1OC OEGPRYNGFWGMMV-UHFFFAOYSA-N 0.000 description 2
- 125000002133 (4-hydroxy-3-iodo-5-nitrophenyl)acetyl group Chemical group OC1=C(C=C(C=C1I)CC(=O)*)[N+](=O)[O-] 0.000 description 2
- 208000006545 Chronic Obstructive Pulmonary Disease Diseases 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 235000019082 Osmanthus Nutrition 0.000 description 2
- 241000333181 Osmanthus Species 0.000 description 2
- 201000007100 Pharyngitis Diseases 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 2
- 244000153955 Reynoutria sachalinensis Species 0.000 description 2
- 235000003202 Reynoutria sachalinensis Nutrition 0.000 description 2
- 206010039085 Rhinitis allergic Diseases 0.000 description 2
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 2
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 2
- 235000009754 Vitis X bourquina Nutrition 0.000 description 2
- 235000012333 Vitis X labruscana Nutrition 0.000 description 2
- 240000006365 Vitis vinifera Species 0.000 description 2
- 235000014787 Vitis vinifera Nutrition 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 208000037883 airway inflammation Diseases 0.000 description 2
- 201000010105 allergic rhinitis Diseases 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000012627 chemopreventive agent Substances 0.000 description 2
- 229940124443 chemopreventive agent Drugs 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000002158 endotoxin Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003862 glucocorticoid Substances 0.000 description 2
- 229920006008 lipopolysaccharide Polymers 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229920000344 molecularly imprinted polymer Polymers 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- -1 polyphenol compound Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 239000011265 semifinished product Substances 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 229940116269 uric acid Drugs 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- HSTZMXCBWJGKHG-UHFFFAOYSA-N (E)-piceid Natural products OC1C(O)C(O)C(CO)OC1OC1=CC(O)=CC(C=CC=2C=CC(O)=CC=2)=C1 HSTZMXCBWJGKHG-UHFFFAOYSA-N 0.000 description 1
- PSYGHMBJXWRQFD-UHFFFAOYSA-N 2-(2-sulfanylacetyl)oxyethyl 2-sulfanylacetate Chemical compound SCC(=O)OCCOC(=O)CS PSYGHMBJXWRQFD-UHFFFAOYSA-N 0.000 description 1
- 241000205585 Aquilegia canadensis Species 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 240000006122 Chenopodium album Species 0.000 description 1
- 235000009344 Chenopodium album Nutrition 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 235000018959 Chrysanthemum indicum Nutrition 0.000 description 1
- 244000179525 Dracocephalum moldavica Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 102100037850 Interferon gamma Human genes 0.000 description 1
- 108010074328 Interferon-gamma Proteins 0.000 description 1
- 108090001005 Interleukin-6 Proteins 0.000 description 1
- 102000003945 NF-kappa B Human genes 0.000 description 1
- 108010057466 NF-kappa B Proteins 0.000 description 1
- 235000004347 Perilla Nutrition 0.000 description 1
- 244000124853 Perilla frutescens Species 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 241001061106 Sargocentron rubrum Species 0.000 description 1
- 206010041954 Starvation Diseases 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 102100040247 Tumor necrosis factor Human genes 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
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- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
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- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 1
- 208000013116 chronic cough Diseases 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 208000037976 chronic inflammation Diseases 0.000 description 1
- 230000006020 chronic inflammation Effects 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 230000016396 cytokine production Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000018823 dietary intake Nutrition 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940000406 drug candidate Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- STVZJERGLQHEKB-UHFFFAOYSA-N ethylene glycol dimethacrylate Chemical compound CC(=C)C(=O)OCCOC(=O)C(C)=C STVZJERGLQHEKB-UHFFFAOYSA-N 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
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- 239000004744 fabric Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
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- 208000026435 phlegm Diseases 0.000 description 1
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- 238000006366 phosphorylation reaction Methods 0.000 description 1
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- 229960003764 polydatin Drugs 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
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- 230000002035 prolonged effect Effects 0.000 description 1
- 108010002730 protein S precursor Proteins 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 235000020095 red wine Nutrition 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- KZJWDPNRJALLNS-VJSFXXLFSA-N sitosterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CC[C@@H](CC)C(C)C)[C@@]1(C)CC2 KZJWDPNRJALLNS-VJSFXXLFSA-N 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
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- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- HSTZMXCBWJGKHG-CUYWLFDKSA-N trans-piceid Polymers O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=CC(\C=C\C=2C=CC(O)=CC=2)=C1 HSTZMXCBWJGKHG-CUYWLFDKSA-N 0.000 description 1
- 230000024883 vasodilation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/004—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring by obtaining phenols from plant material or from animal material
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/30—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only not hydrogenated in the hetero ring, e.g. flavones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/40—Separation, e.g. from natural material; Purification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of health products containing resveratrol and cyanidenon and its preparation method and application.In the health products, including by resveratrol extract and the mixture of cyanidenon extract, especially the two is 1 according to mass ratio:The mixture of 1 composition, further obtains health products in good taste with cloudy mulberry juice, citric acid, D-sorbite and/or edible glycerol interworking.The health products not only have comprehensive healthcare function, have fragrance, and delicious taste, drink conveniently, meet consumer taste demand.
Description
Technical field
The present invention relates to health product technology field, in particular it relates to a kind of health products containing resveratrol and cyanidenon
And its preparation method and application.
Background technology
Resveratrol is polyphenol compound, is mainly derived from the plants such as peanut, grape (red wine), giant knotweed, mulberry fruit.
Resveratrol is a kind of biological very strong natural polyphenol class material, also known as resvertrol, is the chemopreventive agent of tumour, and
To reducing platelet aggregation, prevention and treatment atherosclerosis, the chemopreventive agent of cardiovascular and cerebrovascular disease.United States Department of Agriculture
Result of study shows that peanut red coat in benevolence with also containing considerable resveratrol.2014, scientist took in white lamb's-quarters to human body
General mortality rate caused by the horizontal and various chronic disease of reed alcohol is researched and analysed, and is as a result found, dietary int ake resveratrol and
Longevity, inflammation, cancer and cardiovascular health do not have obvious correlation.
Cyanidenon, it is present in more in the form of glucosides in various plants, these plants are with dragonhead, capsicum, mother chrysanthemum
Flower, honeysuckle, purple perilla content are higher.With antibechic and phlegm-dispelling functions.According to current research, respiratory symptom cough, expectoration, asthma
Breath, it is relevant with chronic airway inflammation.Such as bronchial astehma, COPD, chronic pharyngitis, allergic rhinitis are drawn
Play cough, expectoration, pant, be all considered to, the presence of inflammation so that the immune response of air flue mix relevant with the inflammatory infiltration of part
Disorderly, often there is airway reactivity and increased in patient.The means for the treatment of should eliminate the chronic inflammation infiltration of air flue first.It is conventional at present
Glucocorticoid, to eliminate airway inflammation.But the side effect of glucocorticoid is more, unsuitable prolonged application.Research shows, sweet-scented osmanthus
Careless element suppresses macrophage phosphorylation, suppresses transcription factor NF-kB activity, can suppress the macrophage of lipopolysaccharides (LPS) induction
Cell cytokine production IL-6, TNF-a.In addition, research also indicate that, reseda be known as suppress uric acid generation into promote uric acid
The effect of excretion.Latter two cell factor plays the part of very important role in inflammation mechanism, is the sensitivity for reflecting degree of inflammation
Index.Cyanidenon can also improve IFN-γ, reduce specific Ig-E, reduce the infiltration of eosinophil.In addition, reseda
Element also has in addition to anti-inflammatory, anti-allergic effects and suppresses PDE, anti-SARS, inhibition of HIV characteristic, the reason for this is that suppressing SARS diseases
The activity of malicious Pre-S protein, so as to prevent it from entering host cell.For treat COPD, bronchial astehma and chronic pharyngitis,
Chronic cough caused by allergic rhinitis etc..
As the improvement of people's living standards, its health care consciousness also gradually steps up, and in future, need of the people for health products
Asking will be further urgent and strong.If using resveratrol and cyanidenon as main active component, it is prepared with guarantor
The Foods or drinkses of strong effect, it will be expected to obtain the health products containing natural antioxidant, there is good development prospect.
Roots of Peanut, stem shell are the discarded object after autumn harvest again in China's wide material sources, as extraction resveratrol and wood
The raw material of rhinoceros grass element, Yin Gen and stem had both been avoided to mitigate in giant knotweed, grape etc. again because useless burning causes a large amount of environmental pollutions
The use pressure of medicinal material or fruits and vegetables.
The extraction process of existing resveratrol and cyanidenon there is no the resveratrol and sweet-scented osmanthus of high-purity, high activity
Careless element, operation is more complicated, and recovery rate is also not high enough, limits the activity application of resveratrol and cyanidenon to a certain extent
And the research application of relevant healthcare product.
The content of the invention
The technical problem to be solved in the present invention is the health products for the active component containing resveratrol and cyanidenon
A kind of technical deficiency, there is provided health products containing resveratrol and cyanidenon.
Another technical problem to be solved by the present invention is that provide the preparation method of the health products.
A present invention also technical problems to be solved are to provide the application of the health products.
The purpose of the present invention is achieved by technical scheme once：
A kind of health products containing resveratrol and cyanidenon are provided, in the health products, including are extracted by resveratrol
The mixture of thing and cyanidenon extract composition.
Preferably, in the health products, including by resveratrol extract and cyanidenon extract it is according to mass ratio
1:The mixture of 1 composition.
It is further preferred that in the health products, in addition to appropriate cloudy mulberry juice, citric acid, D-sorbite and/or edible
Glycerine.
It is highly preferred that in the health products containing resveratrol and cyanidenon, by resveratrol extract, cyanidenon
Extract, cloudy mulberry juice, citric acid, D-sorbite and edible glycerol are according to 0.075 part：0.075 part：30 parts：0.3 part：0.5 part：
0.5 part of mass ratio composition.
The preparation method of the health products is that each component mixing is shaken up into homogeneous to produce.
Preferably, the trans-resveratrol contained in the resveratrol extract is 0.083% or more.
Preferably, the preparation method of the resveratrol extract comprises the following steps：
S01. peanut rhizome is selected, is crushed；
S02. liquid ratio 25 is pressed:1 by crushing peanuts rhizome be immersed in concentration of volume percent be 65~75% it is edible
In level ethanol, multiple lucifuge extraction, gained extract solution rotary evaporated to dryness, resveratrol extract is obtained.
Preferably, peanut rhizome described in step S01 is the rhizome of the peanut of Guangdong Zengcheng production.Further preferably it is planted in wide
The rhizome of the peanut of eastern Zengcheng.
Preferably, it is to be crushed to 100 mesh sieves to be crushed described in step S01.
Preferably, the volume ratio percent concentration of ethanol described in step S02 is 70%.
Preferably, the temperature extracted described in step S02 is 50 DEG C, and each extraction time is 1h.
Preferably, step S02 is by liquid ratio 25:Crushing peanuts rhizome is immersed in concentration of volume percent as 65 by 1
In~75% food grade ethanol, then regulation system is extracted to faintly acid, then repeatedly.
It is further preferred that the pH value that the faintly acid is system is 4.8.
Further, the regulation system to faintly acid is to use concentration to be adjusted for 0.1mol/L hydrochloric acid solution
Section.
The optimal extracting method of the resveratrol extract is prepared using extract by solvents, described ethanol
Concentration of volume percent is 70%, liquid ratio 25:1, Extracting temperature：50 DEG C, extraction time 1h, carry out second extraction, time
And 1h.Under the extraction conditions optimized herein, the recovery rate of resveratrol is 0.25%.
It is further preferred that the preparation method of the resveratrol extract also includes following purification step：
S03. resveratrol extract step S02 extracted, by methanol dissolving that may be less；
S04. the mixture after step S03 is dissolved is adsorbed with DA-201 macroreticular resins, then with 60%~100%
Food grade ethanol resveratrol is eluted, collect various concentrations section eluent, rotary evaporation recovery eluant, eluent, obtain
Resveratrol Primary purification product；
S05. by chromatographic column on resveratrol Primary purification product obtained by step S04, filler is compound adsorbent, is washed
It is de-, eluent is collected, obtains resveratrol eluent；The preferred ZK-606 of compound adsorbent (molecularly imprinted polymer) and 400
The mixture of mesh silica gel (presses 1:1 mixing).
S06. after resveratrol eluent obtained by step S05 is concentrated, filtered after adding charcoal absorption, then use stone
Oily ether carries out sorption extraction, collects petroleum ether extraction liquid, freeze-drying, obtains the resveratrol extract that purity is more than 98%
Sterling.
Preferably, elution described in step S04 is that resveratrol is carried out with the ethanol that volume by volume concentration is 70%~100%
Elution.
It is further preferred that it is tracked detection with thin-layer chromatography and high performance liquid chromatography during the elution.
Eluant, eluent described in step S05 is chloroform：The mixture of methanol different volumes ratio, chloroform：Methanol=2:1、3:1、
4:1、5:1、6:1、7:1 or 8:1；It is preferred that use chloroform：Methanol=5:1 mixture.Elution process thin-layer chromatography with efficiently
Liquid chromatography is tracked detection.
Resveratrol purity after the eluent freeze-drying being prepared under the conditions of present invention extraction and purifying process
For more than 98%.
Preferably, the preparation method of the cyanidenon extract comprises the following steps：
S11. using peanut shell as raw material, peanut shell is crushed；It is preferred that cross 50 mesh sieves；
S12. by the peanut shell that step S11 is crushed using 60%~80% food grade ethanol as extract solution, liquid ratio 25:
1, soak extraction 24h under conditions of being 4.8 in 80 DEG C, pH value, collect extract solution, and filter residue soaks 2~3h and carries out second extraction again
Rotary evaporation.Produce cyanidenon extract.
Present invention determine that the optimal extraction process of cyanidenon is：The volumetric concentration of ethanol is 70%, pH value 4.8,
Liquid ratio is 25:1, Extracting temperature：70 DEG C, extraction time 1h, carry out second extraction, time 1h.The recovery rate of cyanidenon
For：0.20%.The superiority of present invention process is demonstrated with design orthogonal test through experiment of single factor.
It is further preferred that the preparation method of the cyanidenon extract also includes following purification step：
S13. cyanidenon extract step S12 extracted, by methanol dissolving that may be less；
S14. the mixture after step S13 is dissolved is adsorbed with DA-201 macroreticular resins, then with 60%~100%
Food grade ethanol cyanidenon is eluted, collect various concentrations section eluent, rotary evaporation recovery eluant, eluent, obtain
Cyanidenon Primary purification product；
Preferably, the peanut shell described in step S11 is the shell for the peanut for being planted in Guangdong Zengcheng.
It is that peanut shell powder was broken into 40~60 mesh sieves to be crushed described in step S11, preferably crosses 50 mesh sieves.
Preferably, elution described in step S14 is to reseda with the food grade ethanol that volume by volume concentration is 70%~100%
Element is eluted.
It is further preferred that it is tracked detection with thin-layer chromatography and high performance liquid chromatography during the elution.
Cyanidenon purity after the eluent freeze-drying being prepared under the conditions of present invention extraction and purifying process
For more than 40%.
In the health products containing resveratrol extract and cyanidenon extract, in the resveratrol extract
The purity of resveratrol is 98% or more, and the purity of cyanidenon is 40% or more in the cyanidenon extract.
Also containing pros-and-cons type resveratrol, pros-and-cons type polydatin, cyanidenon in the health products.
The resveratrol extract and cyanidenon extract system being prepared using extraction preparation method of the present invention
In standby health products, according to weight/mass percentage composition meter, containing formal resveratrol 0.0167%, trans-resveratrol 0.083%, and
Cyanidenon 0.150%.
The beneficial effects of the invention are as follows：
The invention provides a kind of health products with health role (beverage), due to containing science ratio group in extract
Into resveratrol and cyanidenon, the two plays good synergistic function, forms a kind of natural antioxidant, can be clear
Except interior free yl, the anti-aging gene of activating human body, anti-aging, recover the normal function of physiology, antifatigue, the activating cell of strength
Recover young vigor；Moreover, also containing cupreol in peanut rhizome extract, it has the obvious work(for reducing serum cholesterol
Effect.It is sticky that health products of the present invention can reduce blood, suppresses coagulating platelets and vasodilation, keeps blood Channel Group, can be pre-
The generation and development of anti-cancer.
Invention further provides the technical scheme of optimization, with resveratrol extract, cyanidenon extract, sorosis
Juice, citric acid, D-sorbite, edible glycerol compatibility.Under the conditions of the technical scheme of optimization, the health products not only have comprehensive
Healthcare function, and there is fragrance, delicious taste, drink conveniently, meet consumer taste demand.
On this basis, present invention optimizes the preparation method of resveratrol extract and cyanidenon extract, adopt
Resveratrol extract and cyanidenon extract are prepared with extract by solvents, the process conditions of solvent extraction method is optimized, has
Effect, which reduces, prepares cost, and obtains preferable recovery rate.
Present invention determine that optimal Extraction Process of Resveratrol From Ploygonum is：The volumetric concentration of ethanol is 70%, liquid ratio 25:1,
Extracting temperature：50 DEG C, extraction time 1h, second extraction is carried out, the time is also 1h.The recovery rate of resveratrol is：0.25%.Through
Experiment of single factor demonstrates the superiority of present invention process with design orthogonal test.
In terms of extraction, in order to improve recovery rate and under reducing the double effectses guaranteed conditions of production cost, the present invention
Using the solvent extraction method of optimization, the recovery rate of resveratrol is 0.25%, and preparation process is simple, is also walked for subsequent purification
Rapid simplification lays the first stone.In the crude separation of resveratrol, we are purified with DA-201 to the crude extract of resveratrol,
In loading flow velocity 1.00mL/min, elution flow rate 1.60mL/min, volume fraction of ethanol 80%, its after purification resveratrol
Rate is (83.13 ± 0.01) %, and 40% can be reached by detecting its purity through HPLC.In terms of using column chromatography purifying, the present invention
Filler is compound adsorbent (ZK-606+400 mesh silica gel), is eluted, and collects eluent, obtains resveratrol eluent, is added
Filtered after charcoal absorption, then sorption extraction is carried out with petroleum ether, collect petroleum ether extraction liquid, freeze-drying, obtaining purity is
More than 98% resveratrol extract sterling, better assure that the beneficial effect of health products of the present invention.
Present invention determine that the optimal extraction process of cyanidenon is：The volumetric concentration of ethanol is 70%, pH value 4.8,
Liquid ratio is 25:1, Extracting temperature：70 DEG C, extraction time 1h, carry out second extraction, time 1h.The recovery rate of cyanidenon
For：0.20%.The superiority of present invention process is demonstrated with design orthogonal test through experiment of single factor.
Brief description of the drawings
The HPLC chromatogram of Fig. 1 resveratrol standard specimens.
The HPLC chromatogram of Fig. 2 Zengchengs peanut rhizome resveratrol extract.
The HPLC chromatogram of the resveratrol standard specimen of Fig. 3 embodiments 4.
The HPLC chromatogram of the resveratrol standard specimen of Fig. 4 ZhongKai Agriculture Engineering Academy general plantings.
Fig. 5 embodiment 5DA-201 macroporous resin adsorption results.
Fig. 6 embodiments 5 elute result.
The testing result of the gained resveratrol extract sterling of Fig. 7 embodiments 5.
The HPLC chromatogram of the cyanidenon standard specimen of Fig. 8 embodiments 6.
The HPLC chromatogram of the extract of the peanut shell of Fig. 9 Guangdong Zengcheng plantation.
The external anticancer of carry out of Figure 10 peanut rhizome resveratrol extracts and peanut shell cyanidenon extract mixtures
Experimental result (blank and medication 24 hours).
The external anticancer of carry out of Figure 11 peanut rhizome resveratrol extracts and peanut shell cyanidenon extract mixtures
Experimental result (blank and medication 48 hours).
The external anticancer of carry out of Figure 12 peanut rhizome resveratrol extracts and peanut shell cyanidenon extract mixtures
Experimental result (blank and medication 72 hours).
Embodiment
The inventive method is further illustrated with reference to specific embodiment.Following being given for example only property of embodiment explanations, no
It is understood that as limitation of the present invention.Unless stated otherwise, biomaterial, the reagent raw material used in following embodiments is conventional
The biomaterial and reagent raw material that purchased in market or commercial sources obtain, unless stated otherwise, the method that is used in following embodiments and
Equipment is method and apparatus commonly used in the art.
Embodiment 1
S01. the rhizome of the peanut of Guangdong Zengcheng plantation is selected, crushed after being dried crosses 100 mesh sieves；
S02. liquid ratio 25 is pressed:1 is immersed in crushing peanuts rhizome in 65% food grade ethanol, 50 DEG C of multiple lucifuges
Extraction, extraction 1 hour, merges gained extract solution rotary evaporated to dryness, obtains resveratrol extract every time.
Embodiment 2
S01. the rhizome of the peanut of Guangdong Zengcheng plantation is selected, crushed after being dried crosses 100 mesh sieves；
S02. liquid ratio 25 is pressed:1 is immersed in crushing peanuts rhizome in 70% food grade ethanol, 50 DEG C of multiple lucifuges
Extraction, extraction 1 hour, merges gained extract solution rotary evaporated to dryness, obtains resveratrol extract every time.
Embodiment 3
S01. the rhizome of the peanut of Guangdong Zengcheng plantation is selected, crushed after being dried crosses 50 mesh sieves；
S02. liquid ratio 25 is pressed:1 is immersed in crushing peanuts rhizome in 75% food grade ethanol, 50 DEG C of multiple lucifuges
Extraction, extraction 1 hour, merges gained extract solution rotary evaporated to dryness, obtains resveratrol extract every time.
Any gained resveratrol extract of Example 1 to 3 carries out HPLC detections.Fig. 1 is resveratrol standard specimen
HPLC chromatogram, Fig. 2 are the HPLC chromatogram that the present invention uses Zengcheng peanut rhizome resveratrol extract, as seen from the figure, this
Resveratrol containing high level in invention extract.
Embodiment 4
S01. select general planting peanut rhizome (by ZhongKai Agriculture Engineering Academy plantation peanut exemplified by illustrate, but
The species of peanut is not limited), crushed after being dried crosses 50 mesh sieves；
S02. liquid ratio 25 is pressed:1 is immersed in crushing peanuts rhizome the food grade second that concentration of volume percent is 70%
In alcohol, 50 DEG C of multiple lucifuge extractions, extraction 1 hour, merges gained extract solution rotary evaporated to dryness, obtains resveratrol and carry every time
Take thing.
The gained resveratrol extract of Example 4 carries out HPLC detections.Fig. 3 is the HPLC chromatogram of resveratrol standard specimen
Figure, Fig. 4 are the HPLC chromatogram that the present embodiment uses Zengcheng peanut rhizome resveratrol extract.
Embodiment 5
S01. the rhizome of the peanut of Guangdong Zengcheng plantation is selected, crushed after being dried crosses 100 mesh sieves；
S02. liquid ratio 25 is pressed:1 is immersed in crushing peanuts rhizome in 70% food grade ethanol, 50 DEG C of multiple lucifuges
Extraction, extraction 1 hour, merges gained extract solution rotary evaporated to dryness, obtains resveratrol extract every time；
S03. by S02 resveratrol extract rotary evaporated to dryness, dissolved with a small amount of methanol, be freeze-dried to doing, obtain
Just extraction product；
S04. will just extract product obtained by step S03 to be adsorbed on macroreticular resin, macroreticular resin model used
DA-201；Then gradient elution is carried out on macroreticular resin with 60%, 70%, 80%, 90%, 100% ethanol, eluent is used
Thin-layered chromatography and high performance liquid chromatography are tracked, and collect the eluent of resveratrol section；By the solution of resveratrol section
Concentration and recovery ethanol, obtain the resveratrol semi-finished product that content is 45% or so；Fig. 5 embodiment 5DA-201 macroporous resin adsorptions
As a result as shown in accompanying drawing 5, elute shown in result as accompanying drawing 6.
S05. it is compound adsorbent (ZK-606+400 mesh silica gel) by chromatographic column on gained semi-finished product, filler, uses chlorine successively
It is imitative：Methanol is 2:1、3:1、4:1、5:1、6:1、7:1、8:1 volume ratio is eluted, with thin-layered chromatography and high-efficient liquid phase color
Spectrometry is tracked.Collect the eluent of resveratrol section.
S06. after resveratrol eluent obtained by step S05 is concentrated, filtered after adding charcoal absorption, then use stone
Oily ether carries out sorption extraction, collects petroleum ether extraction liquid, freeze-drying, obtains the resveratrol extract that purity is more than 98%
Sterling.Shown in testing result as accompanying drawing 7.
Embodiment 6
S11. the shell of the peanut of Guangdong Zengcheng plantation is selected, crushed after being dried crosses 50 mesh sieves；
S12. liquid ratio 25 is pressed:1 is immersed in crushing peanuts shell in 60% food grade ethanol, 60 DEG C of repeatedly extractions,
Extraction 24 hours every time, merge gained extract solution rotary evaporated to dryness, obtain cyanidenon extract.
Embodiment 7
S11. the shell of the peanut of Guangdong Zengcheng plantation is selected, crushed after being dried crosses 50 mesh sieves；
S12. liquid ratio 25 is pressed:1 is immersed in crushing peanuts shell in 70% food grade ethanol, 60 DEG C of repeatedly extractions,
Extraction 24 hours every time, merge gained extract solution rotary evaporated to dryness, obtain cyanidenon extract.
Embodiment 8
S11. the shell of the peanut of Guangdong Zengcheng plantation is selected, crushed after being dried crosses 50 mesh sieves；
S12. liquid ratio 25 is pressed:1 is immersed in crushing peanuts shell in 75% food grade ethanol, 60 DEG C of repeatedly extractions,
Extraction 24 hours every time, merge gained extract solution rotary evaporated to dryness, obtain cyanidenon extract.
As shown in Figure 9, Fig. 8 is cyanidenon to the HPLC chromatogram of any gained cyanidenon extract of embodiment 6 to 8
The HPLC chromatogram of standard specimen.
Embodiment 9
S11. the shell of the peanut of selection selection general planting is (by taking the peanut of ZhongKai Agriculture Engineering Academy plantation as an example, but not
Limit the species of peanut), crushed after being dried crosses 50 mesh sieves；
S12. liquid ratio 25 is pressed:1 is immersed in crushing peanuts shell in 100% food grade ethanol, 60 DEG C of repeatedly extractions,
Extraction 24 hours every time, merge gained extract solution rotary evaporated to dryness, obtain cyanidenon extract.
Embodiment 10
S11. the shell of the peanut of Guangdong Zengcheng plantation is selected, crushed after being dried crosses 50 mesh sieves；
S12. liquid ratio 25 is pressed:1 is immersed in crushing peanuts shell in 70% food grade ethanol, 60 DEG C of repeatedly extractions,
Extraction 24 hours every time, merge gained extract solution rotary evaporated to dryness, obtain cyanidenon extract；
S13. by S02 cyanidenon extract rotary evaporated to dryness, dissolved with a small amount of methanol, be freeze-dried to doing, obtain
Just extraction product.
Embodiment 11
The present embodiment provides a kind of health products containing resveratrol and cyanidenon, in the health products, including following matter
Measure each component of number：Resveratrol extract made from any example of Example 1 to 5, any example of Example 6 to 10 are made
Cyanidenon extract, cloudy mulberry juice purchased in market, citric acid, D-sorbite, edible glycerol, according to the mass fraction shown in table 1
Formula rate, each component is mixed, shaken up, homogeneous produces.
Table 1
Embodiment 12
Detection product A~H's the results are shown in Table shown in 2.Select 10 subjective appreciation composition of personnel evaluation groups, it is desirable to which sense organ is commented
Fix the number of workers 12h before evaluation does not drink, and non-smoking, does not eat pungent irritable food of Denging, and 2 samples will be gargled with clear water before and after evaluation
Mouthful simultaneously it is spaced 10min, the mode such as taste using range estimation, nasil, mouth carries out subjective appreciation one by one, is allocated as, for optimal number, filling out with 10
Write results of sensory evaluation.Detection and results of sensory evaluation see the table below 2.
Table 2
Embodiment 13
According to mass fraction ratio it is 1 with peanut rhizome resveratrol extract and peanut shell cyanidenon extract:1
After ratio mixing, external anticancer experiment is carried out, it is found that extract plays the role of suppression to cell, as shown in Figure 10 to 12, has attached
Scheme visible, cell quantity has obvious difference before and after dosing, a large amount of apoptosis of cell after dosing.
The preparation of the ZK-606 adsorbents of embodiment 14
Therefore the material and instrument that the present embodiment uses as shown in Table 3 and Table 4, but do not limit the scope of the invention.
The experimental drug table of table 3
The laboratory apparatus table of table 4
Molecularly imprinted polymer is prepared using precipitation polymerization method：
S1. a certain amount of template molecule resveratrol and function monomer methacrylic acid (MAA) are weighed, it is molten to be dissolved in methanol
In agent；
S2. the cyclotron oscillation prepolymerization 1h in thermostatic control oscillator vibration, crosslinking agent GDMA is added
(EGDMA) and 0.1g initiator azodiisobutyronitriles (AIBN), after ultrasonic deoxidation 10min, N is led to210min starvations, it is close
Envelope, polymerize 24h in water bath with thermostatic control.
S3. polymer is taken out, the filtering of cloth funnel, dried.With V (methanol):V (acetic acid)=8:2 are used as eluent, rope
Formula extracts a week, washes away template molecule, dries, that is, obtains resveratrol imprinted polymer (MIPs), be named as ZK-606.
Under conditions of template molecule resveratrol is not added with, blank imprinted polymer (NIPs) is made in the same way.
30mg each 6 parts of ZK-606 (MIPs21) and NIPs21 is weighed respectively, and it is about the white of 1~8mmol/L to add concentration
Veratryl alcohol methanol solution, cyclotron oscillation absorption 24h, surveys its adsorbance at room temperature.
ZK-606 Selective adsorption result of the test is shown in Table 4：
Adsorbances of the MIPs of table 4 from NIPs to different substrates
Claims (10)
1. a kind of health products containing resveratrol and cyanidenon, it is characterised in that in the health products, including by resveratrol
The mixture of extract and cyanidenon extract composition.
2. the health products containing resveratrol and cyanidenon according to claim 1, it is characterised in that in the health products,
Including according to mass ratio being 1 by resveratrol extract and cyanidenon extract:The mixture of 1 composition.
3. the health products according to claim 1 or claim 2 containing resveratrol and cyanidenon, it is characterised in that the health products
In, in addition to appropriate cloudy mulberry juice, citric acid, D-sorbite and/or edible glycerol；It is preferred that
In the health products containing resveratrol and cyanidenon, by resveratrol extract, cyanidenon extract, sorosis
Juice, citric acid, D-sorbite and edible glycerol are according to 0.075 part：0.075 part：30 parts：0.3 part：0.5 part：0.5 part of quality
Than composition.
4. the health products containing resveratrol and cyanidenon according to any one of claims 1 to 3, it is characterised in that described
The preparation method of resveratrol extract comprises the following steps：
S01. peanut rhizome is selected, is crushed, sieving；
S02. liquid ratio 25 is pressed:1 is immersed in crushing peanuts rhizome the food grade ethanol that concentration of volume percent is 65~75%
In, multiple lucifuge extraction, gained extract solution rotary evaporated to dryness, obtain resveratrol extract.
5. the health products containing resveratrol and cyanidenon according to claim 4, it is characterised in that mistake described in step S01
Sieve was 100 mesh sieves；The concentration of volume percent of food grade ethanol described in step S02 is 70%.
6. the health products containing resveratrol and cyanidenon according to claim 4 or 5, it is characterised in that the white black false hellebore
The preparation method of alcohol extracting thing is further comprising the steps of：
S03. resveratrol extract step S02 extracted, use methanol dissolving as few as possible；
S04. the mixture after step S03 is dissolved is adsorbed with DA-201 macroreticular resins, then with 60%~100% it is edible
Level ethanol elutes to resveratrol, collects the eluent of various concentrations section, rotary evaporation recovery eluant, eluent, obtains white black false hellebore
Alcohol Primary purification product；
S05. by chromatographic column on resveratrol Primary purification product obtained by step S04, filler is compound adsorbent resveratrol point
Sub- imprinted polymer ZK-606 and 400 mesh silica-gel mixtures, are eluted, and are collected eluent, are obtained resveratrol eluent；
S06. after resveratrol eluent obtained by step S05 is concentrated, filtered after adding charcoal absorption, then use petroleum ether
Sorption extraction is carried out, petroleum ether extraction liquid is collected, freeze-drying, obtains the resveratrol extract sterling that purity is more than 98%.
7. the health products containing resveratrol and cyanidenon according to any one of claims 1 to 3, it is characterised in that described
The preparation method of cyanidenon extract comprises the following steps：
S11. using peanut shell as raw material, peanut shell is crushed, sieved；
S12. by the peanut shell that step S11 is crushed using 60%~80% food grade ethanol as extract solution, liquid ratio 25,80
DEG C, pH value be 4.8 under conditions of soak extraction, collect extract solution, filter residue soak again carry out second extraction rotary evaporation, produce wood
Rhinoceros grass extract.
8. the health products containing resveratrol and cyanidenon according to claim 7, it is characterised in that mistake described in step S11
Sieve was 50 mesh sieves；The soak extraction time described in step S12 is 24 hours, and soak time is 2~3 hours to filter residue again.
9. the health products containing resveratrol and cyanidenon according to claim 7, it is characterised in that the resveratrol carries
Take the preparation method of thing further comprising the steps of：
S13. cyanidenon extract step S12 extracted, use methanol dissolving as few as possible；
S14. the mixture after step S13 is dissolved is adsorbed with DA-201 macroreticular resins, then with 60%~100% it is edible
Level ethanol elutes to cyanidenon, collects the eluent of various concentrations section, rotary evaporation recovery eluant, eluent, obtains reseda
Plain Primary purification product.
10. the health products containing resveratrol and cyanidenon according to any one of claim 1 to 9, it is characterised in that described
In health products, according to weight/mass percentage composition meter, containing formal resveratrol and glycosides 0.0167%, trans-resveratrol and glycosides 0.083%
With the % of cyanidenon 0.150.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109750078A (en) * | 2019-01-16 | 2019-05-14 | 南京师范大学 | A kind of method and application of composite bacteria agent bioconversion peanut waste effectively synthesizing resveratrol coproduction luteolin |
| CN114751394A (en) * | 2022-05-05 | 2022-07-15 | 兰州文理学院 | Preparation of nitrogen-doped pepper residue biomass porous carbon material and application of nitrogen-doped pepper residue biomass porous carbon material as electrode material |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005126429A (en) * | 2003-09-30 | 2005-05-19 | Japan Science & Technology Agency | Immunopotentiating agent, food and liquid food composition |
| CN101074188A (en) * | 2007-04-17 | 2007-11-21 | 河南省农科院农副产品加工研究所 | Method for enriching and purifying veralkcohol from peanut root by macporous adsorptive resin |
| CN102040579A (en) * | 2011-01-25 | 2011-05-04 | 彭国平 | Method for extracting luteolin from peanut roots, stems, leaves and shells |
| CN106794214A (en) * | 2014-10-20 | 2017-05-31 | 瓦尔比奥蒂斯公司 | The composition of the mixture of contained molecule in mixture containing plant extracts or the plant, and for controlling the purposes of carbohydrate and/or lipid-metabolism |
| CN107198232A (en) * | 2017-06-21 | 2017-09-26 | 浏阳市红杉农业科技发展有限公司 | A kind of health products promoted longevity |
-
2017
- 2017-10-10 CN CN201710946012.7A patent/CN107692230A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005126429A (en) * | 2003-09-30 | 2005-05-19 | Japan Science & Technology Agency | Immunopotentiating agent, food and liquid food composition |
| CN101074188A (en) * | 2007-04-17 | 2007-11-21 | 河南省农科院农副产品加工研究所 | Method for enriching and purifying veralkcohol from peanut root by macporous adsorptive resin |
| CN102040579A (en) * | 2011-01-25 | 2011-05-04 | 彭国平 | Method for extracting luteolin from peanut roots, stems, leaves and shells |
| CN106794214A (en) * | 2014-10-20 | 2017-05-31 | 瓦尔比奥蒂斯公司 | The composition of the mixture of contained molecule in mixture containing plant extracts or the plant, and for controlling the purposes of carbohydrate and/or lipid-metabolism |
| CN107198232A (en) * | 2017-06-21 | 2017-09-26 | 浏阳市红杉农业科技发展有限公司 | A kind of health products promoted longevity |
Non-Patent Citations (5)
| Title |
|---|
| 向海艳等: ""反式白藜芦醇分子印迹复合膜的制备及其选择性"", 《应用化学》 * |
| 向海艳等: ""选择性富集分离虎杖中白藜芦醇苷的分子印迹聚合物的制备及分子识别性能研究"", 《分析测试学报》 * |
| 杨新玲: "《吃对颜色才健康:五色蔬果饮食养生全书》", 31 March 2013, 青岛出版社 * |
| 段玉清等: ""白藜芦醇纳米二氧化硅表面分子印迹聚合物的制备及吸附特性"", 《食品科学》 * |
| 金青哲: "《功能性脂质》", 31 August 2013, 中国轻工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109750078A (en) * | 2019-01-16 | 2019-05-14 | 南京师范大学 | A kind of method and application of composite bacteria agent bioconversion peanut waste effectively synthesizing resveratrol coproduction luteolin |
| CN114751394A (en) * | 2022-05-05 | 2022-07-15 | 兰州文理学院 | Preparation of nitrogen-doped pepper residue biomass porous carbon material and application of nitrogen-doped pepper residue biomass porous carbon material as electrode material |
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