CN107823237A - A kind of extracting method of burdock root total flavone - Google Patents
A kind of extracting method of burdock root total flavone Download PDFInfo
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The present invention program is related to a kind of extracting method of burdock root total flavone, including crushes drying：Radix bardanae pulverizing medicinal materials are taken into 8 mesh, and kept dry；Ethanol extracts：By dried radix bardanae and ethanol solution with 1g:12~24ml solid-liquid ratio, refluxing extraction three times, 3.5~4.5 hours every time, obtains extract solution at a temperature of 50~70 DEG C；Filtering and concentrating：The extract solution filtering that step 2 is obtained and merging filtrate, are evaporated concentration to filtrate using rotary evaporator, obtain concentrate；Petroleum ether extraction：Using petroleum ether to concentrate ungrease treatment, dried after obtaining degreasing concentrate, form crude extract.This programme, which is realized, completes extraction and separation process research, and has preliminary pharmacological tests, while there has also been certain Research Thinking and desk study result of the test to the separation purifying technique of some key active ingredients.
Description
Technical field
The invention belongs to botanical herbses extractive technique field, and in particular to one kind utilizes plant radix bardanae particle extraction chemistry
The extracting method of general flavone.
Background technology
Burdock（Arctium Lappa L.）China is originated in, is Compositae Arctium lappa category biennial herb plant.Radix bardanae is ox
The meat taproot of burdock, return lung warp；The heart channel of Hang-Shaoyin.Cure mainly：Wind-dispelling heat, detumescence poison.Wind toxin edema of the face is controlled, dizzy, throat heat is swollen, dentalgia, cough
Cough, quench one's thirst, ulcer sore scabies.In recent years, the medical value of radix bardanae was more and more paid attention in the area such as America and Europe, Japan, research hair
Rich in protein, carbohydrate, Polyphenols, arctic acid, polyacetylene material and polysaccharose substance etc. in existing radix bardanae, have anti-
Bacterium, anti-mutation, anticancer is anti-oxidant, Liver protection, removes the different physiological roles such as heavy metal ion.This programme is based on to radix bardanae
The optimum process condition of total more ketone extractions in the analysis of traditional Chinese medicine ingredients, preferably radix bardanae, be further development functionality food and
Feature Chinese medicine and value provide foundation.
The content of the invention
It is an object of the invention to based on the research to radix bardanae composition, propose a kind of extraction side of burdock root total flavone
Method, and realize to its purification process.
The present invention program proposes a kind of extracting method of burdock root total flavone, comprises the following steps：
Step 1: crush drying：Radix bardanae pulverizing medicinal materials are taken into 8 mesh, and kept dry；
Step 2: ethanol extracts：By dried radix bardanae and ethanol solution with 1g:12~24ml solid-liquid ratio, 50~70
Refluxing extraction three times, 3.5~4.5 hours every time, obtains extract solution at a temperature of DEG C；
Step 3: filtering and concentrating：The extract solution filtering that step 2 is obtained and merging filtrate, are entered using rotary evaporator to filtrate
Row is concentrated by evaporation, and obtains concentrate；
Step 4: petroleum ether extraction：Using petroleum ether to concentrate ungrease treatment, dry, formed thick after obtaining degreasing concentrate
Extract.
Further scheme, Step 5: isolating and purifying：Crude extract prepared by step 4 is prepared into 0.01g/mL burdock
The root total flavone aqueous solution, macroreticular resin is placed in the burdock root total flavone aqueous solution, and is put into Static Adsorption in constant temperature oscillator
1-4h, obtain the burdock root total flavone of preliminary purification.
Further scheme, the step 5 also include：Into the burdock root total flavone aqueous solution after absorption add 15%~
60% ethanol is 1.25~2.75 BV/h as eluant, eluent, elution speed, and eluent is collected after elution.
Further scheme, the step 5 also include step 6 afterwards：
Step 6: freeze-drying：Crude extract is sufficiently stirred, to prevent crude extract surface to be hardened, treats that crude extract volume is basically unchanged
When, carry out freeze-drying and form extract dry powder.
Further scheme, the constant temperature oscillator keep 20 DEG C of constant temperature.
Further scheme, the elution speed are 1.25~2.75 BV/h.
Further scheme, the drying device in the S4 is baking oven, and it is 75~85 to carry out oven temperature during step S4
DEG C forced air drying.
Further scheme, concentration of alcohol is 50% in the S5.
This programme has the beneficial effect that：
1st, this programme proposes that the extracting method of radix bardanae extraction general flavone takes full advantage of plant component and realizes chemical composition of Chinese materia medica
Extraction.This programme, which is realized, completes extraction and separation process research, and has preliminary pharmacological tests, while crucial to some
The separation purifying technique of active ingredient there has also been certain Research Thinking and desk study result of the test, and this programme passes through expansion first
Lab scale research technique, the repeatability and reliability of checking lab scale research are improved in big experimental study.
2nd, this programme has carried out purification process after realizing total more ketone extractions, and macroporous absorbent resin extracts burdock root total flavone
Thing carries out once after purification, carrying out secondarily purified guarantee DNA purity；
3rd, Flavonoid substances in radix bardanae are extracted using ethanol as Extraction solvent, are easy to commercial Application, concentration of alcohol is observed, carries
Time and solid-liquid ratio these three factors for mainly influenceing recovery rate are taken to do orthogonal test and choose relatively average extraction process, with reed
Fourth is standard items, is developed the color using natrium nitrosum, aluminum nitrate, sodium hydroxide, solution determines trap at 510nm and calculates every group
Content is extracted, so that it is determined that optimum extraction process, can be as the reference of industrial manufacture process.
Brief description of the drawings
Fig. 1 is the step flow chart of the present invention program always extraction of more ketone；
Fig. 2 is the technology path block diagram of the extraction of the total more ketone of the present invention.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, below in conjunction with accompanying drawing and specific implementation
Mode, the present invention will be described in further detail.It should be appreciated that embodiment described herein is only explaining this
Invention, is not intended to limit the present invention.
As shown in Fig. 1 Fig. 2, the present invention program proposes a kind of extracting method of burdock root total flavone, comprises the following steps：
Step 1: S1, crushing drying：Radix bardanae pulverizing medicinal materials are taken into 8 mesh, and kept dry；
Step 2: S2, ethanol extract：By dried radix bardanae and ethanol solution with 1g:12~24ml solid-liquid ratio, 50~
Refluxing extraction three times, 3.5~4.5 hours every time, obtains extract solution at a temperature of 70 DEG C；
Step 3: S3, filtering and concentrating：The extract solution filtering that step 2 is obtained and merging filtrate, using rotary evaporator to filter
Liquid is evaporated concentration, obtains concentrate；
Step 4: S3, petroleum ether extraction：Using petroleum ether to concentrate ungrease treatment, dry, formed after obtaining degreasing concentrate
Crude extract.
Further scheme, Step 5: isolating and purifying：Crude extract prepared by step 4 is prepared into 0.01g/mL burdock
The root total flavone aqueous solution, macroreticular resin is placed in the burdock root total flavone aqueous solution, and is put into Static Adsorption 1 in constant temperature oscillator
~4h, obtain the burdock root total flavone of preliminary purification.
Further scheme, the step 5 also include：Into the burdock root total flavone aqueous solution after absorption add 15%~
60% ethanol is 1.25~-2.75 BV/h as eluant, eluent, elution speed, and eluent is collected after elution.
Further scheme, the step 5 also include step 6 afterwards：
Step 6: freeze-drying：Crude extract is sufficiently stirred, to prevent crude extract surface to be hardened, treats that crude extract volume is basically unchanged
When, carry out freeze-drying and form extract dry powder.
Further scheme, the constant temperature oscillator keep 20 DEG C of constant temperature.
Further scheme, the elution speed are 1.25~2.75 BV/h.
Further scheme, the drying device in the S4 is baking oven, and it is 75~85 to carry out oven temperature during step S4
DEG C forced air drying.
Further scheme, concentration of alcohol is 50% in the S5.
Specifically this programme is described in detail with reference to case：
Take crude drug 20.0g to crush, the mesh of granularity 8, kept dry is standby, is divided to two groups, every group of refluxing extraction three times, merging filtrate,
Filter.Filtrate, which is transferred in revolving, is concentrated by evaporation recovery ethanol to without alcohol taste, with petroleum ether extraction, refrigerates standby.Interval is certain
Time is stirred, and prevents thick paste surface is hardened from influenceing evaporation effect, and thick paste volume is freeze-dried when being basically unchanged, and is claimed after drying
Weight, is put into standby in drier.
About 10.0mg extract dry powders are taken, adds the dissolving of 30% ethanol to be settled to 50mL volumetric flasks, draws 1mL, 2.5mL, 5mL extremely
In color-comparison tube.Every group of processing method according to standard items is handled.And the quality of the dry extract powder taken before and total
Ratio between dry extract quality obtains the every group of flavones extracted gross mass.
The accurate radix bardanae 20.0g for weighing the drying of 8 mesh sieves, solid-liquid ratio 1 is pressed with 60% ethanol:18（m:v）At 60 DEG C
Extraction 4 hours, every group of refluxing extraction three times, merging filtrate, filter.Filtrate, which is transferred in revolving, is concentrated by evaporation recovery ethanol to nothing
Alcohol taste, according to the amount of liquid medicine finally evaporated, make last amount of liquid medicine control in 10mL or so.Petroleum ether extraction, it is thick to obtain radix bardanae
Powder.Refrigerate standby.
Macroreticular resin Dynamic Adsorption calculates with the related physical quantity in elution process, being related to mainly adsorbance, absorption
Rate, calculation formula are as follows.
Every gram of adsorbance=(C0V0-C1V1)/W
Adsorption rate=(C0V0-C1V1)/C0V0 × 100%
Desorption efficiency (P)=C2V2/ (C0V0-C1V1) × 100%
C0 in formula:Loading mass concentration (mg/mL);C1:The mass concentration (mg/mL) of raffinate after absorption;C2 is stripping liquid
Mass concentration;V0:The volume (mL) of loading;V1:Surplus solution volume (mL) after absorption;V2 is stripping liquid volume
(mL) ; W:Resin quality (g).
Accurately the 4 kinds of each 4.0g of fresh resin D101, AB-8, LS-46D, NKA-9 filtered are weighed in the three of 500mL
In pyramid bottle, the 400mL10mg/mL burdock root total flavone aqueous solution is separately added into, is put into constant temperature oscillator static suction
It is attached, 25 DEG C, frequency of oscillation 100rpm of temperature is set, shakes 4h.Respectively determine 0.5h, 1.0h, 1.5h, 2h, 2.5h, 3h, 3.5h,
The content of the flavones of sample liquid during 4h.After absorption terminates, removing Liquid Residue is filtered under diminished pressure, respectively adds 50mL into conical flask respectively
30% ethanol, static desorption is put into constant temperature oscillator, shakes 4h, carries out the parsing of flavones, determines content.To Static Adsorption
Equilibrium liquid and stripping liquid general flavone concentration are measured, and are calculated adsorption rate, resolution factor, are compared and filter out preferable resin.
Resin pre-processes：
By the macroporous absorbent resin newly purchased first with distilled water immersion to remove water-solubility impurity and suspension, then with 95% ethanol
24h is soaked, no alcohol taste is washed to distillation again fully after swelling and aqueous is clarified, and then carry out soda acid processing.First with 2 times of cylinders
The 5% watery hydrochloric acid immersion 12h of product (BV), it is neutral to be washed to pH value with distillation；Again with 5% hydrogen-oxygen of 2 times of column volumes (BV)
Change sodium solution immersion 12h, be washed to pH value neutrality with distillation, dry rear standby.
5% dilute hydrochloric acid solution immersion resin is first added in container 12 hours, eluted after filling post with same concentration hydrochloric acid solution
2~3 times of column volumes, neutrality then is washed to distillation, then resin is soaked 12 hours with 5% sodium hydroxide solution, used after filling post
Eluted with sodium hydroxide solution, be finally washed to neutrality with distillation.
20.0mg rutin standard items accurately are weighed, is dissolved in 20mL60% ethanol, ultrasonic dissolution, is settled to 60% ethanol
100mL.7 clean tool plug test tube numberings are taken, by the reagent preparation of table 2.3, shakes after adding natrium nitrosum, adds after standing 6 minutes
Aluminum nitrate, shaking, 6 minutes back end hydrogenation sodium oxide molybdenas is stood again, 15 minutes is stood after shaking, is finally settled to 30% ethanol
10ml.Returned to zero using No. 1 as blank, absorbance is determined at 510nm wavelength.Using rutin content as abscissa, using absorbance as
Ordinate, it is fitted to obtain calibration curve equation:Y=13.62x+0.0131, R2=0.9982, in good linear relationship.
LS-46D resin 80.0g are taken, wet method is fitted into (3 cm × 30cm) tool valve chromatographic column, investigation sample solution concentration,
Applied sample amount, influence of the eluant, eluent flow velocity to purifying general flavone, optimize technique.
The difference of eluant, eluent determining alcohol, the effect of flavones purifying are different.Add 15%, 30%, 45%, 60% ethanol with water respectively
Upper excellent chromatographic column is eluted.
Best using 30% ethanol elution effect, general flavone purity reaches 53.3%, higher than the ethanol of other concentration, so choosing
Eluted with 30% ethanol.
Elution flow rate is different, and it is different to reach eluting agent needed for identical elution effect.By 4g samples by the suction optimized
Sub conditione upper prop adsorbs, elution, with 2500ml30% ethanol respectively with 1.25,1.5,2.0,2.25,2.5,2.75 BV/h flow velocitys
Elution, eluent is collected, determine the concentration of flavones in eluent, and calculate resolution factor, it is determined that optimal eluant, eluent flow velocity.
Macroreticular resin 46-D is to general flavone resolution factor as eluant, eluent speed increase is gradually reduced, it is contemplated that the production cycle,
It is that 1.5BV/h ratios are convenient to select elution speed.
Dynamic purification technique is；Crude extract（Concentration）Sample concentration 0.01g/ml, applied sample amount 4g,（Applied sample amount --- tree
Fat amount）Eluant, eluent is 30% ethanol, the BV/h of eluent speed 1.5;General flavone purity is up to 53.3% after macroporous resin purification
, taken a firm foundation for further research.
No alcohol taste is washed to distillation after polyamide is soaked into 24h with 95% ethanol, then is soaked with 4%NaOH solution
24h, upper strata alkali lye is filtered, then neutrality is washed to distillation, then soak 24h with 4%HCl, then neutrality is washed to distillation, steamed
Distilled water immersion is standby.
Radix bardanae dry powder is taken, is dissolved in water, it is 0.40g.mL to obtain crude drug mass concentration-1Solution.
With the increase of polyamide mesh number, adsorbance increase, and eluting rate, purity are then on the contrary.This is due to polyamides
The increase of amine mesh number, its area contacted with feed liquid also accordingly increases, therefore its adsorbance is in rising trend；But due to mesh
Number is bigger, and absorption of the single polyamide molecule to polyphenol is closer, causes elution more difficult, and in the larger polyamide of mesh number
Containing thinner polyamide powder, easily together flowed out with eluent, influence the purity of polyphenol；The bigger polyamide of mesh number is more
Difficulty is pre-processed.Therefore, consider, column chromatography resin of 60~80 mesh polyamide as this experiment should be selected.
Under room temperature (25 DEG C), the dry g of polyamide 0.30 is accurately weighed, is put in centrifuge tube, add the mL of water 20 to activate
The h of resin 4, make its saturation that absorbs water, filter superfluous water, it is 0.05,0.15,0.2,0.25,0.3 to be separately added into mass concentration,
0.35,0.4g.mL-1, every time shakes 1 time, adsorbs 24 h, it is fully balanced.
General flavone mass concentration in equilibrium liquid is determined, it is respectively 38.5,71.6,102.8 to calculate adsorbance (Q),
130.6,156.9,174.1,182.4,189.6mg.g-1.Therefore selection loading mass concentration 0.4g.mL-1.
Flavone compound is polyhydroxy phenols, and in faintly acid, therefore pH has certain influence to its absorption property.This experiment
The absorption situation that polyamide is respectively 1.0,3.0,5.0,7.0,9.0,11.0 sample solution to pH is investigated.At room temperature
Accurate to weigh polyamide 0.30g, concrete operations add 0.4gmL with 2.3.1 items-1Sample solution 10mL, with HCl or NaOH
Solution adjusts pH value of solution, as a result Q be respectively 308.5,380.6,293.2,160.5,73.2,13.5mgg-1.PH is to adsorbance
Have a great influence, during pH3 or so, adsorbance is big;In pH ＜ 3 acid adsorption liquid, Adsorbent rate is reduced and subtracted with pH
It is small, thus it is speculated that because under certain acidity, the amide groups in polyamide molecule can combine proton and protonation reaction occurs, and gather
Amide resin absorption flavone compound is the carbonyl adsorption capacity for relying on the amido link in polyamide;As pH ＞ 3, gather
Hydrogen bond is formed with the phenolic hydroxyl group in flavone compound and is adsorbed, so the low pH of solution reduces the amide resin of polyamide
Adsorption capacity is raised and declined with pH.Because flavone compound contains phenolic hydroxyl group mostly, therefore aobvious acid, increase with pH, flavones
The faintly acid of class compound is gradually neutralized, and phenolic hydroxyl group is ionized in solution, therefore flavone compound is adsorbed under ability
Drop.So too high or too low pH is unfavorable for the absorption of polyamide, this experiment selection pH3.
By above-mentioned adsorption conditionses, sample solution is preceding with the elution of 6BV deionized waters by resin column after precision measures processing
3BV collects 1 part per BV, and rear 3BV merges into portion, is successively 20% with volume fraction, 40%, 60%, 80% each 20mL of ethanol
Elution, eluent flow rate 1mLmin-1Collect eluent respectively, measure water elution thing dry cream quality is respectively 0.064,0.028,
0.006、0.032;It is respectively 59.6%, 90.8%, 92.8%, 88.5% that general flavone, which adds up eluting rate, in ethanol eluate.By result
Understand, it is 92.8% that 60% ethanol, which adds up eluting rate, therefore determines that eluting solvent is 60% ethanol.
According to above-mentioned adsorption conditionses, sample solution, with 3BV water elutions, measures 60% by resin column after precision measures processing
Ethanol 50mL is eluted, eluent flow rate 1mLmin-1, Fractional Collections eluent, prepare need testing solution, measure general flavone parsing
Rate is respectively 65.1%%, 81.8%, 87.4%, 92.9%, 97.1%, result show that 4BV60% ethanol can reach 92.9% eluting rate, therefore
It is 4BV to determine 60% ethanol consumption.
Concentration is the 0.40 g/mL mL of radix bardanae crude flavonoid powder solution 10, with 0.5 mL/min loading flow velocity loadings, is inhaled
After attached 30 min, with 3 BV water elution impurity, respectively with 3 BV 60% ethanol elution solution, with 1,2,3,4,5
BV/h flow velocity (i.e. 0.25,0.5,0.75,1,1.25 mL/min flow velocitys) crosses post elution, collects 3 BV ethanol flavones respectively
Eluent, measure flavones eluting rate different in flow rate.With the raising of elution flow rate, the resolution factor of Celery is lifted, and 4
BV/h (1mL/min) flow velocity Celery eluting rate is maximum, to 5 BV/h flow velocity when resolution factor decline on the contrary.This be by
In the quickening of speed, the Flavonoid substances that ethanol eluate can not be well with adsorbing on resin are caused to swap, thus not
Elution effect well can be reached.Therefore, it is advisable from 4 BV/h (1 mL/min) flow velocity elution radix bardanae flavones.
Polyamide is the organic high poly- adsorbent of one kind, with selectivity is good, adsorption capacity is big, it is easy etc. excellent to desorb
Point, the enriching and purifying applied to phenolic hydroxy group class compound have preferable effect.This research is tested by Static Adsorption, either
Static adsorbance or static desorption efficiency, polyamide is all more satisfactory, and by dynamic test, it is dense further to optimize eluting solvent
The flow velocity of degree, eluting agent and ethanol elution, after burdock root total flavone is isolated and purified by polyamide, flavones content by
The 53.3% of crude product brings up to 89.1%, and purity improves 1.67 times, to be wherein adapted to industrialized production to provide reliable science
Foundation.
The present embodiment experiment shows:Pulverizing medicinal materials take 20.0g medicinal powder solubilizer to extract 3 times, alcohol reflux carries to 8 mesh
Merging extract solution is taken, is filtered, concentration and recovery ethanol, petroleum ether degreasing 3 times.Powder is freeze-dried into, absorbance is surveyed after dissolving and is calculated
Go out flavones content.The optimal extract process finally determined is:Extraction time is 4 hours, and concentration of alcohol is 60% and 1:18
Solid-liquid ratio, extractive total flavone 11.3%.
The present invention program has preferable application prospect, and this programme provides theoretical trajectory for the extraction of general flavone, for industry
Technical foundation is established in mass production, can be to solve current China's Chinese material medicine resource to enrich, but the utilization of resources is short of this contradiction.To draw
A kind of dynamic China's Development of pharmaceutical industry, there is provided new solution route.Also local farmers are improved by planting Chinese herbal medicine to shake off poverty and set out on the road to prosperity
Enthusiasm, promote the development of Chinese herbal medicine planting base local economy, play the quick hair that strength promotes China's modernization of Chinese medicine
The effect of exhibition.
Succeeding in developing for product will necessarily be to solve current China's Chinese material medicine resource enrich, but product it is single, without characteristic
Pillar product pulls contradiction between China's Development of pharmaceutical industry to provide a kind of new solution route, and China's traditional Chinese medical science is driven with this
The development of medicine industry, and the enthusiasm that local farmers are shaken off poverty and set out on the road to prosperity by planting Chinese herbal medicine is further improved, promote medium-height grass pharmacopoeia
Phytyl local economy development, play strength promote China's modernization of Chinese medicine fast-developing effect.
It should be appreciated that although the present specification is described in terms of embodiments, not each embodiment only includes one
Individual independent technical scheme, this narrating mode of specification is only that those skilled in the art will should say for clarity
Bright book is as an entirety, and the technical solutions in the various embodiments may also be suitably combined, and forming those skilled in the art can be with
The other embodiment of understanding.
Claims (8)
- A kind of 1. extracting method of burdock root total flavone, it is characterised in that：Comprise the following steps：Step 1: crush drying：Radix bardanae pulverizing medicinal materials are taken into 8 mesh, and kept dry；Step 2: ethanol extracts：By dried radix bardanae and ethanol solution with 1g:12~24ml solid-liquid ratio, 50~70 Refluxing extraction three times, 3.5~4.5 hours every time, obtains extract solution at a temperature of DEG C；Step 3: filtering and concentrating：The extract solution filtering that step 2 is obtained and merging filtrate, are entered using rotary evaporator to filtrate Row is concentrated by evaporation, and obtains concentrate；Step 4: petroleum ether extraction：Using petroleum ether to concentrate ungrease treatment, dry, formed thick after obtaining degreasing concentrate Extract.
- 2. a kind of extracting method of burdock root total flavone as claimed in claim 1, it is characterised in that also include：Step 5: isolate and purify：Crude extract prepared by step 4 is prepared into the 0.01g/mL burdock root total flavone aqueous solution, will Macroreticular resin is placed in the burdock root total flavone aqueous solution, and is put into Static Adsorption 1-4h in constant temperature oscillator, obtains preliminary purification Burdock root total flavone.
- 3. a kind of extracting method of burdock root total flavone as claimed in claim 2, it is characterised in that the step 5 is also wrapped Include：15%~60% ethanol is added into the burdock root total flavone aqueous solution after absorption and is used as eluant, eluent, elution speed for 1.25~ 2.75 BV/h, eluent is collected after elution.
- A kind of 4. extracting method of burdock root total flavone as claimed in claim 2 or claim 3, it is characterised in that the step 5 it Also include step 6 afterwards：Step 6: freeze-drying：Crude extract is sufficiently stirred, to prevent crude extract surface to be hardened, treats that crude extract volume is basically unchanged When, carry out freeze-drying and form extract dry powder.
- 5. a kind of extracting method of burdock root total flavone as claimed in claim 2, it is characterised in that the constant temperature oscillator is protected Hold 20 DEG C of constant temperature.
- 6. a kind of extracting method of burdock root total flavone as claimed in claim 3, it is characterised in that the elution speed is 1.25~2.75 BV/h.
- 7. the extracting method of the total more ketone of radix bardanae extraction is utilized as claimed in claim 1, it is characterised in that：It is dry in the S4 Dry device is baking oven, and it is 75~85 DEG C of forced air dryings to carry out oven temperature during step S4.
- 8. the extracting method of the total more ketone of radix bardanae extraction is utilized as claimed in claim 1, it is characterised in that：Ethanol in the S5 Concentration is 50%.
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