CN107668048A - A kind of composite bactericide for preventing and treating graw mold of tomato - Google Patents
A kind of composite bactericide for preventing and treating graw mold of tomato Download PDFInfo
- Publication number
- CN107668048A CN107668048A CN201710896860.1A CN201710896860A CN107668048A CN 107668048 A CN107668048 A CN 107668048A CN 201710896860 A CN201710896860 A CN 201710896860A CN 107668048 A CN107668048 A CN 107668048A
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- China
- Prior art keywords
- tetramycin
- fluazinam
- tomato
- preventing
- graw mold
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
Abstract
The invention discloses a kind of compound disinfectant for preventing and treating graw mold of tomato, belongs to biological pesticide preparaton research and development field.The compound disinfectant is using tetramycin and fluazinam as base-material, according to tetramycin and fluazinam 7:1~1:7 quality proportioning is formulated, and preferably tetramycin and fluazinam quality proportioning are 3:1.The compound disinfectant of the present invention, two kinds of base-materials do not chemically react, with obvious synergy or it is added effect, it is safe to use, there is good cytotoxicity for the different types of gray mold in current field, and apparently higher than tetramycin or fluazinam single dose, it is used to prevent and treat graw mold of tomato suitable for vast rural area.
Description
Technical field
The invention belongs to biological pesticide preparaton to research and develop field, and in particular to a kind of compound sterilizing for preventing and treating graw mold of tomato
Agent.
Background technology
Graw mold of tomato is a kind of world's venereal disease as caused by Botrytis cinerea (Botrytis cinerea Pers.exFr.)
Evil, endangers on facilities vegetable and is particularly acute, and can cause a large amount of decayed fruits, and chemical prevention is to mitigate the primary hand that gray mold causes harm
Section.The medicament of preventing and treating gray mold mainly has benzimidazole germicide (carbendazim, thiophanate-methyl), N- phenyl amino first at present
Bactericide of phosphate (phosphinium) ester (diethofencarb), dicarboximide fungicide (procymidone, iprodione), anilino-pyrimidine series bactericidal agent are (phonetic
Mould amine), Bravo and Mancozeb, preceding 4 class medicament because Botrytis cinerea is drug-fast produce greatly reduce prevention effect.
Thus biological control turns into a kind of important supplement means for substituting chemical prevention, but biological control, due to slowly effect, effect is not
The factors such as stabilization, so as to influence its application prospect, turn into the best mode of both mutual benefits using biological-chemical collaboration preventing and treating.Base
In this thinking, the present invention carries out the composition research of biological-chemical collaboration preventing and treating by screening efficient biological bactericide.Close
The biological-chemical of reason is bactericide compounded or mixture has expansion fungicidal spectrum, improves prevention effect, extends the optimum period for applying fertilizer, reduces and use
Dose, poisoning is reduced, residual is reduced, delays Antifungal resistance and drug-fast generation, being the maximally efficient of solution problem above
One of method.
Tetramycin (tetramycin) is the fermentating metabolism product of S. ahygroscopicus Wuzhou subspecies, and it includes A1, A2, B and C
Four components, wherein A1 and A2 are four dilute antibiotic of macrolide;B is pyrimidine nucleotides antibiotic;C is nitrogen heterocyclic ring
Fragrant family antibiotic.It is respectively provided with protection and therapeutic activity to the plurality of plant diseases on the crops such as cereal, fruit tree, vegetables, especially
It is to gray mold (crop such as tomato, cucumber, capsicum), powdery mildew (wheat class, melon, fruit tree etc.), scab (apple, pears etc.) etc.
Fungal disease has good prevention effect.
Fluazinam (fluazinam), belong to 2,6 dinitroanilines, be the new sterilization of Japanese Shi Yuan companies exploitation
Agent, to interlink spore genus, Botrytis, Phytophthora, Plasmopara, Sclerotinia and it is black to cultivate Pseudomonas highly effective, resist benzo miaow
The Botrytis cinerea of azole and dicarboximide series bactericidal agent also has good result, resistance of rainwater washing against, lasting period length, has concurrently excellent
The effect of control food plant property mite class, also there is the preventive effect of brilliance to crucifer clubroot, to the rice as caused by rhizopus
Samping off also has good preventive effect.It is usually used in preventing and treating capsicum epidemic disease, potato and tomato late blight etc., is a kind of good protection
Property bactericide.Its mechanism of action is to suppress all stages by suppressing spore germination, mycelia breakthrough, growth and sporogenesis
Course of infection.It is but hindered in actually promoting because its price is high.
The content of the invention
It is an object of the invention to provide a kind of compound disinfectant for preventing and treating graw mold of tomato, and it has to botrytis cinerea
Standby stronger poisoning ability, preventing and treating graw mold of tomato can be applied to, increase the vegetables industry volume increase for accounting for agricultural product critical role
Receive, be the development service of national economy.
Inventor carries out repeated screening to the biological pesticide with killing botrytis cinerea activity, and to Mixed Pharmacy
Bulk drug quality proportioning carries out repetition test, and result of the test is contrasted, a kind of compound disinfectant is found, to graw mold of tomato
Bacterium possesses stronger poisoning ability.
Determined by toxicity, inventor has found EC of the tetramycin to the mycelial growth of ash arrhizus bacteria50It is worth for 0.23 μ g/
Ml, and especially significantly suppress conidia germination, to the EC of conidia germination50It is worth for 0.008 μ g/ml;Meanwhile the present invention
People studies resistance risk of the ash arrhizus bacteria to tetramycin, it is found that tetramycin is different from the high other any selections of resistance risk
Property antibiotic and chemical bactericide, can not screen tetramycin resistance botrytis cinerea under laboratory and field condition.Thus push away
Disconnected, tetramycin is likely present other special resistant effect mechanism.
A kind of composite bactericide for preventing and treating graw mold of tomato, using tetramycin and fluazinam as base-material, according to tetramycin and fluorine
The quality proportioning 7 of pyridine amine:1~1:7 are formulated.
Preferably, a kind of composite bactericide for preventing and treating graw mold of tomato as described above, tetramycin and fluazinam it is optimal
Quality proportioning is 3:1.
Compared with prior art, advantages of the present invention is:1. composition greatly reduces chemical medication after both are compounded
Amount, prevention effect are far above single dose, have the function that to subtract medicine synergy, meet the needs of current China's agricultural supply side reform;2.
By by biological-chemical it is bactericide compounded after, effectively reduce the resistance risk of chemical bactericide, while improve the speed of biological agent
Effect property.
Embodiment
For the technical characterstic for illustrating the present invention program can be understood, with reference to specific embodiment, the present invention is illustrated.
But protection scope of the present invention is not limited to these embodiments.It is every equal without departing substantially from the change of present inventive concept or equivalent substitute
It is included within protection scope of the present invention.
Inventor has carried out substantial amounts of experiment for the compounded combination of tetramycin and fluazinam, and specific experiment is as follows:
The toxicity test of experiment 1, fluazinam and tetramycin mixture to graw mold of tomato
Using the botrytis cinerea to fluazinam sensitivity as experiment target, indoor measurement tetramycin and fluazinam different proportion
Composition to the virulence and interaction coefficient of synergism of target.Its method is dissolved into 1000 μ using 95% fluazinam active compound with methanol
G/mL mother liquors, the active compound of tetramycin are diluted with water 1000 μ g/mL mother liquors, saved backup at 4 DEG C.Tetramycin and fluazinam are pressed
Effective content is configured to 1 respectively:7、1:5、1:3、1:1、3:1、5:1、7:The serial test solution of 1 mixture, is then mixed into culture matrix
(PDA) inhibitory activity to tomato gray mould bacterial strain mycelial growth, is determined, calculates EC50Value and coefficient of synergism.At DPS data
Reason system, calculates regression equation and EC50Value.By Wadley methods, coefficient of synergism (SR) is calculated.
Mycelial growth average inhibition=[(control colony diameter average-processing colony diameter average)/(control bacterium colony is straight
Footpath average-inoculation bacteria cake diameter)] × 100%.
The synergy type of pharmacy mix is evaluated according to coefficient of synergism (SR), i.e. SR < 0.5 are antagonism, 0.5≤
SR≤1.5 are summation action, and SR > 1.5 are synergistic effect.SR=EC50(Eth)/EC50(ob), EC50(Eth)=(a+b)/
[(a/EC50A)+(b/EC50B)].Wherein, A, B are respectively bactericide single dose, and a, b are ratio of the corresponding single dose in mixture, EC50
(Eth) it is mixture EC50Theoretical value, EC50(Eob) it is mixture EC50Measured value.
It is can be seen that according to the result of table 1 when the proportioning of tetramycin and fluazinam is 1~7:When 7~1, to graw mold of tomato
The EC of bacterium50For 0.0209~0.218 μ g/ml, coefficient of synergism is 1.85~5.14, has reached the effect of synergy.
The tetramycin fluazinam formula indoor the selection result (resistant strain BJ47) of table 1
Medicament | Regression equation | Coefficient correlation | EC50(μg/ml) | Coefficient of synergism |
Tetramycin (A) | Y=1.4213x+5.9013 | R2=0.9315 | 0.23 | |
Fluazinam (B) | Y=1.4213x+5.9013 | R2=0.9748 | 0.082 | |
A:B(1:7) | Y=0.7472x+5.810 | R2=0.9883 | 0.034 | 2.65 |
A:B(1:5) | Y=0.6186x+5.9116 | R2=0.9954 | 0.048 | 1.91 |
A:B(1:3) | Y=0.7542x+5.993 | R2=0.9707 | 0.039 | 2.52 |
A:B(1:1) | Y=1.0207x+6.4403 | R2=0.9863 | 0.039 | 3.14 |
A:B(3:1) | Y=0.9264x+6.310 | R2=0.9955 | 0.021 | 7.60 |
A:B(5:1) | Y=1.0079x+6.6931 | R2=0.9957 | 0.055 | 3.22 |
A:B(7:1) | Y=1.1158x+6.4059 | R2=0.9569 | 0.083 | 2.26 |
Example of formulations 1:20% tetramycin fluazinam wettable powder
Tetramycin 15%, fluazinam 5%, NNO-11%, pull open powder 3%, neopelex 2%, attapulgite
30%th, remaining is that precipitated calcium carbonate is supplemented to 100%.Said components are fully mixed, 300 mesh sieves are crushed and crossed through sand mill,
Obtain 20% tetramycin fluazinam wettable powder.
Example of formulations 2:18% tetramycin fluazinam suspending agent
Tetramycin 4%, fluazinam 14%, ethylene glycol 4%, propane diols 4%, the dispersant of NNO-11%, NNO-7 1%, Huang
Virgin rubber sticker 0.5%, polyethylene glycol 1%, remaining is that water is supplemented to 100%, said components is fully mixed, through sand mill powder
90% pharmacy particle diameter≤5 μm are broken to, obtain 18% tetramycin fluazinam suspending agent.
Example of formulations 3:The micro- floating agent of 18% tetramycin fluazinam
Tetramycin 10%, fluazinam 8%, 1-METHYLPYRROLIDONE 1%, alkyl benzene calcium sulfonate 2%, phenethyl phenol formaldehyde tree
Ester polyoxyethyl ether 2%, ethylene glycol 1.5%, silicone compound 0.8%, xanthans 1%, aluminium-magnesium silicate 1%, remaining is water
It is supplemented to 100%.By the tetramycin of aforementioned proportion, fluazinam, solvent N-methyl pyrilidone, emulsifying agent alkyl benzene calcium sulfonate and
Phenethyl phenol mela mine-formaldehyde resin polyoxyethyl ether is added together, and is dissolved into homogeneous oil phase;Water, antifreeze ethylene glycol, thickener is yellow
Virgin rubber, defoamer silicone compound mix, into homogeneous aqueous phase.Under high velocity agitation, aqueous phase is added into oil phase, you can
The micro- floating agent of 18% tetramycin fluazinam is made.
Example of formulations 4:24% tetramycin fluazinam water dispersible granules
Tetramycin 4%, fluazinam 20%, ammonium sulfate 10%, APES 2.5%, DBSA
Sodium 2.5%, polyoxyethylene carboxylate 3.5%, remaining is that precipitated calcium carbonate is supplemented to 100%, and said components are fully mixed,
Female powder is prepared by crushing, female powder is well mixed with the appropriate aqueous solution, high speed shear and sand mill grinding, is then fluidized
Bed is granulated, and is dried, sieving, you can 24% tetramycin fluazinam water dispersible granules are made.
We to the embodiment 1-4 samples prepared prevent and treat the field test of graw mold of tomato, are tested in 2014 1
The moon No. 27 and on March 25th, 2015, test site is respectively Tianjin academy of agricultural sciences high-new technology farming Demonstration Garden and Wuhan City thing lake
Tomato planting base, subjects are respectively graw mold of tomato.If 6 kinds spray processing, clear water is control, and each processing sets 3
Secondary repetition, a cell is once repeated as, plot area is 10 square meters, and cell is arranged according to RANDOMIZED BLOCK DESIGN.Tomato gray mould
The investigation of disease is according to " field control effectiveness test criterion bactericidal agent for preventing and treating eliminating vegetable botrytis GB/T17980.28-2000 " is investigated, in reality
During testing, special weather (rainfall, chilling process etc.) is recorded
Table 2:Tetramycin and fluazinam composition for preventing and controlling graw mold of tomato field test
Table of field test results is bright within 2 years, and the microbicide compositions preparation of embodiment 1~4 divides the preventive effect of graw mold of tomato
Do not reach 93.65% and 88.23%, 89.05% and 86.14%, 87.93% and 84.48%, 88.18% and 85.75%, far
Higher than the prevention effect of single dose tetramycin and fluazinam, microbicide compositions of the invention are sprayed after both are compounded, no
The incidence of disease, disease index are only effectively reduced, improves prevention effect, and greatly reduces dosage, is saved a large amount of
Economic input.
Claims (2)
- A kind of 1. composite bactericide for preventing and treating graw mold of tomato, it is characterised in that:The compound disinfectant is with tetramycin and fluorine pyridine Amine is base-material, according to tetramycin and the mass ratio 7 of fluazinam:1~1:7 are formulated.
- A kind of 2. composite bactericide for preventing and treating graw mold of tomato as claimed in claim 1, it is characterised in that:Tetramycin and fluorine pyridine The quality proportioning 3 of amine:1.
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CN201710896860.1A CN107668048B (en) | 2017-09-28 | 2017-09-28 | Compound bactericide for preventing and treating tomato gray mold |
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CN107668048A true CN107668048A (en) | 2018-02-09 |
CN107668048B CN107668048B (en) | 2020-04-14 |
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Citations (1)
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CN103583532A (en) * | 2013-11-26 | 2014-02-19 | 辽宁微科生物工程有限公司 | Tetramycin complex and application |
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CN103583532A (en) * | 2013-11-26 | 2014-02-19 | 辽宁微科生物工程有限公司 | Tetramycin complex and application |
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