CN107663231B - Nicotiana benthamiana TMP14 protein and application thereof in resisting plant viruses - Google Patents
Nicotiana benthamiana TMP14 protein and application thereof in resisting plant viruses Download PDFInfo
- Publication number
- CN107663231B CN107663231B CN201710667567.8A CN201710667567A CN107663231B CN 107663231 B CN107663231 B CN 107663231B CN 201710667567 A CN201710667567 A CN 201710667567A CN 107663231 B CN107663231 B CN 107663231B
- Authority
- CN
- China
- Prior art keywords
- tmp14
- protein
- nicotiana benthamiana
- tobacco
- tswv
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
Abstract
The invention discloses a Nicotiana benthamiana TMP14 protein and application thereof in resisting plant viruses. The amino acid sequence of the Nicotiana benthamiana TMP14 protein is shown in a sequence table SEQ ID NO 1. The invention also discloses an optimized mutant of the Nicotiana benthamiana TMP14 protein. The TMP14 protein of the invention directly interacts with TSWV NSm, and the over-expression transgenic tobacco plant has the function of resisting plant viruses, so the discovery provides theoretical and practical basis for researching transgenic antiviral crops and has wide application prospect.
Description
Technical Field
The invention belongs to the technical field of molecular biology, and particularly relates to a Bunshi tobacco TMP14 protein and application thereof in resisting plant viruses.
Background
TMP14 is a phosphorylated protein (TMP) that localizes to the thylakoid membrane of chloroplast. Studies on Arabidopsis thaliana TMP14(AtTMP14) showed that the protein is encoded by the nuclear gene and the precursor comprises a chloroplast signal peptide consisting of 45 amino acids. AtTMP14 has a molecular weight of 14kDa, presumably at the N-terminus of the chloroplast stroma, with the central region containing 2 transmembrane helices, and threonine at positions 21 or 22 as the phosphorylation site. TMP14 is a specific protein encoded by photosynthetic organisms such as plants and blue-green algae, but the specific function in photosynthesis has not been known so far. Recent studies have shown that TMP14 is one of the constitutive subunits of the plant photosystem I (PSI) pigment-protein complex, and thus is named PSI-P, which is presumed to play an important role in plant photoreaction. NbTMP14 is the subunit of photosystem-I of Nicotiana benthamiana, and the gene has a total length of 549nt, and the encoded precursor protein contains a chloroplast signal peptide consisting of 20 amino acids.
Tomato Spotted Wilt Virus (TSWV) is a representative species of Tomato spotted wilt virus (Tospovirus) of Bunyaviridae (Bunyaviridae), is transmitted by Frankliniella occidentalis to infect over 1000 host species, causes typical symptoms of chlorosis, necrosis, ring spot and the like in organs such as leaves, stems, fruits and the like of plants, and seriously jeopardizes global agricultural production. NSm is a unique coding protein of tospovir virus, and has a very important role in the interaction process of the tospovir virus and a host plant. The research of TSWV NSm by using biochemical, histochemical and other methods shows that NSm has the typical characteristics of Motor Protein (MP), such as: 1) tightly bound to the cytoplasmic membrane, cell wall, 2) RNA affinity, 3) localized to plasmodesmata, and 4) modified plasmodesmata. At present, there is no report that the TMP14 protein interacts with the TSWV NSm protein and is resistant to plant viruses, and the effect of the TMP14 protein of Nicotiana benthamiana is unknown.
Disclosure of Invention
The invention aims to provide a Nicotiana benthamiana TMP14 protein and a coding gene thereof.
The invention also aims to provide the interaction between the Nicotiana benthamiana TMP14 protein and the TSWV NSm protein and the application thereof in resisting plant viruses.
The amino acid sequence of the Bunshi cigarette TMP14 protein is shown as a sequence table SEQ ID NO 1.
The gene sequence of the Nicotiana benthamiana TMP14 protein is shown in a sequence table SEQ ID NO 2.
The gene vector of the Nicotiana benthamiana TMP14 protein.
The host bacterium of the gene vector of the TMP14 protein of the Nicotiana benthamiana.
A primer for amplifying any fragment of the above mentioned Bhatschen Nicotiana benthamiana TMP14 gene.
The amino acid sequence of the mutant of the cigarette ben TMP14 protein is shown as a sequence table SEQ ID NO 3.
The gene sequence of the mutant of the cigarette TMP14 protein is shown in a sequence table SEQ ID NO 4.
The interaction between the above mentioned native cigarette TMP14 protein and the TSWV NSm protein.
The application of the Nicotiana benthamiana TMP14 protein and the Nicotiana benthamiana TMP14 protein mutant in resisting plant viruses.
The invention has the beneficial effects that: the cigarette TMP14 protein of the invention has direct interaction with TSWV NSm; the over-expression transgenic tobacco plant has the function of resisting plant viruses, provides theoretical and practical basis for researching transgenic antiviral crops, and has wide application prospect; the mutant protein has better anti-plant virus effect.
Drawings
FIG. 1 is a graph of yeast double-hybridization of interaction of Nicotiana benthamiana TMP14 protein and TSWV NSm protein.
FIG. 2 shows the interaction between Nicotiana benthamiana TMP14 and TSWV NSm as verified by the dual fluorescence complementation method.
FIG. 3 TSWV vaccination and VIGS downregulation of TMP14 symptom expression;
in the figure, a.tswv inoculation is compared to healthy plants 10 days after; trv VIGS method silences TMP14 symptom expression; C. qPCR detection of TMP14 expression level 10 days after the inoculation of TSWV; qpcr detects the silencing effect of TMP 14.
FIG. 4 shows the morphology of the T.benthamiana green body after the inoculation of TSWV and the expression of down-regulated TMP14 by electron microscopy.
FIG. 5 shows TSWV symptom expression and virus accumulation detection of Nicotiana benthamiana plants with down-regulated TMP14 expression.
FIG. 6 TMP14 gene transfer, Benshi smoke identification and TSWV challenge test;
in the figure, symptoms manifest a. after inoculation with TSWV10 days; qPCR analysis of TMP14 expression; detecting the accumulation amount of TSWV; and D, detecting the transgenic plant by Western Blot.
Detailed Description
The invention is further described with reference to the following figures and specific embodiments.
Example 1 direct interaction of TSWV NSm with Bunshi Nicotiana tabacum TMP14
Inserting TSWV NSm into pBT3Insertion of STE, NbTMP14 into pPR3-N, to obtain pBT3STE-NSm and pPR3-N-TMP14 two vectors. Both vectors were simultaneously transferred into yeast strain NMY51, simultaneously with pBT3STE-NSm and empty vector pPR3-N, with pPR3-N-TMP14 and the empty vector pBT3STE co-transformed yeasts as two negative controls, yeast-lacking plates were applied and positive clones were identified by PCR on the broth. And (4) shaking the positive clones, collecting thalli, diluting the thalli into different concentrations, further coating the thalli on a yeast three-plate, and observing the growth vigor of the three-plate. The results show that pBT3STE-NSm and pPR3N-TMP14 Co-transformed Yeast are capable of Normal growth on triple-deleted plates, whereas pBT3STE-NSm and empty vector pPR3-N、pPR3-N-TMP14 and empty vector pBT3The STE co-transformed yeast was inhibited, indicating that there was a direct interaction of TSWV NSm with Nicotiana benthamiana TMP14 (FIG. 1).
NbTMP14 and TSWV NSm are respectively inserted into bimolecular fluorescent carriers YFPN and YFPC to obtain two carriers of TMP14-YFPN and NSm-YFPC. The TMP14-YFPN and NSm-YFPC vectors were transformed into Agrobacterium GV 3101. By utilizing an agrobacterium infection mode, TMP14-YFPN and NSm-YFPC agrobacterium are co-injected into Nicotiana benthamiana respectively, and the positioning of TMP14 is observed when about 36h-48 h. It was found that in wild type ben-zen tobacco there is a yellow fluorescent protein and it is localized in chloroplasts, which also further verifies the interaction of TMP14 with NSm and the location of the interaction occurs in chloroplasts (fig. 2).
The above results demonstrate that the native tobacco TMP14 protein interacts directly with TSWV NSm. NSm is an important multifunctional protein encoded by TSWV. Mediates the virus to complete intercellular motility and is associated with the onset of symptoms. The role that TMP14 interacts with in the course of viral infection remains to be investigated.
Example 2 inoculation of TSWV with Nicotiana benthamiana results in down-regulation of TMP14 expression and chloroplast dysplasia
The Nicotiana benthamiana is rubbed and inoculated with the virus TSWV, the disease symptoms of tobacco are observed, and obvious etiolated wilting symptoms appear on leaves of a Nicotiana benthamiana system 10 days after inoculation. Total RNA is extracted from diseased leaves, qPCR is carried out to detect the expression level of TMP14 after RT-PCR confirms virus infection, and the expression level of TMP14 is found to be obviously reduced compared with healthy tobacco (A and C in figure 3).
Meanwhile, the diseased leaves are taken and the cell structure is observed by using an electron microscope, and the chloroplast of the tobacco inoculated with the virus is obviously larger than the starch granule on the chloroplast of the wild tobacco, the structure of the chloroplast is damaged, and the morphology is obviously changed (figure 4).
To further determine the relationship between the onset of viral symptoms and the downregulation of TMP14 expression, the expression was downregulated by means of TRV VIGS. Respectively constructing two vectors of pTRV2-GFP and pTRV2-TMP 14; the tobacco is injected after being respectively mixed with TRV 11: 1 by transforming the agro-pole GV3101, pTRV2-GFP and pTRV2-TMP 14; the phenotype of obvious yellowing appears 10 days after injection, the expression level of TMP14 of the Nicotiana benthamiana is reduced by qPCR (B and D in figure 3), the morphology of chloroplasts is observed by using an electron microscope, and the tobacco chloroplast of the TMP14 silent is seriously damaged compared with the morphology of the chloroplast of the control Nicotiana benthamiana (figure 4).
Example 3 Down-regulation of Byan TMP14 expression favors TSWV infection and Up-regulation increases resistance to it
Up-regulation of TSWV accumulation following TRV VIGS silencing of TMP14 expression
TRV2-GFP and TRV2-TMP14 are mixed with TRV 11: 1 respectively and then injected into tobacco; 10 days after injection, samples were taken to examine the expression level of TMP14 in both tobaccos, and as a result, the expression level of TMP14 in TRV2-TMP14 was found to be significantly lower than the expression level of TMP14 in TRV 2-GFP. The pTRV2-GFP and pTRV2-TMP14 tobaccos were further inoculated with TSWV viruses, and samples were taken 10 days after inoculation of the TSWV viruses in the two tobaccos to examine the accumulation of TSWV in the pTRV2-TMP14 tobaccos, which was found to be significantly higher than the accumulation of TSV in pTRV2-GFP tobaccos (FIG. 5).
2. Transgenic overexpression of TMP14 Benzen tobacco with increased TSWV resistance
Constructing a fusion gene TMP14-GFP, inserting the TMP14-GFP fusion gene into a PBI121 vector to obtain a vector TMP14-GFP-PBI 121; then agrobacterium LBA4404 is transformed to obtain the strain. Finally, a genetic transformation experiment is utilized to transform the TMP14-GFP-PBI121 vector into tissue culture tobacco, and the TMP14-GFP-PBI121 transgenic tobacco seedling TMP14-OE is finally obtained through callus induction, resistant callus screening, resistant callus differentiation and induced rooting. Meanwhile, PCR, protein particle hybridization and Western experiments are utilized to identify the transgenic tobacco as a positive plant. The expression level of TMP14 in the transgenic tobacco and the wild-type tobacco at the same period is detected by qPCR, and the expression level of TMP14 in the transgenic tobacco is obviously higher than that of TMP14 in the wild-type tobacco. The tobacco and wild tobacco are further inoculated with TSWV virus, no obvious symptoms are generated on TMP14-OE plants 10 days after virus inoculation, and obvious leaf curling is generated on wild type Nicotiana benthamiana. Samples were taken from both tobacco plants to determine the accumulation of TSWV, and the accumulation of virus in the transgenic tobacco was found to be significantly lower than that in the wild-type tobacco (FIG. 6).
The results prove that the Nicotiana benthamiana TMP14 plays an important role in the virus infection process, and the expression of the Nicotiana benthamiana TMP14 is down-regulated due to virus infection, so that the normal development of chloroplast is influenced, and severe virus symptoms are formed. When overexpressed in nicotiana benthamiana, resistance to TSWV virus accumulation is compromised. Analyzing the reason, on one hand, the TMP14 is over-expressed to enable the TMP14 to be greatly combined with NSm interaction and interfere the normal function of the NSm; in addition, the over-expression of TMP14 helps to maintain the normal morphology of chloroplasts and prevent the onset of symptoms.
Example 4
The TMP14-GFP-PBI121 plant expression vector is used as a template, a mutant is obtained by an overlap extension PCR method, and LS amino acid residues at the 9 th and 10 th positions of mutation sites are mutated into PP. Reconstructing a plant expression vector TMP14 '-GFP-PBI 121, and transforming tobacco by an agrobacterium-mediated method to obtain a transgenic tobacco plant of TMP 14' gene driven by 35S promoter.
Example 5
Planting TMP14 gene-transferred tobacco and wild tobacco, when the tobacco grows to 60 days and the seedling stage is to be finished, taking tobacco plants with consistent growth size and consistent leaf number, inoculating TSWV virus, and after 10 days after virus inoculation, counting the number of plants generating virus spots (more than 3 obvious spots are generated and recorded as infection pathogenicity).
The experimental results are as follows: the infection rate of TMP14 gene-transferred tobacco was 38%, and the infection rate of wild-type tobacco was 99%.
Example 6
Planting TMP 14' gene-transferred tobacco and wild tobacco, when the tobacco grows to 60 days and the seedling stage is to be finished, taking tobacco plants with consistent growth size and consistent leaf number, inoculating TSWV virus, and after 10 days after virus inoculation, counting the number of plants generating virus spots (more than 3 obvious spots are generated and recorded as infection pathogenicity).
The experimental results are as follows: the infection rate of TMP14 gene-transferred tobacco was 2%, and the infection rate of wild-type tobacco was 99%.
Example 7 aphid (hemipteran) resistance assay
Planting TMP14 gene and TMP 14' gene-transferred tobacco and wild tobacco, wherein the tobacco grows for 60 days, when the seedling stage is to be finished, taking tobacco plants with consistent growth size and consistent leaf number, 30 PtTI gene-transferred tobacco plants and 30 wild tobacco plants, grafting 100 mature wingless aphids on each tobacco, and counting the aphid inhibition rate after 30 hours: the aphid inhibition rate is (100-residual aphids on leaves)/100.
The experimental results are as follows: the aphid inhibition rate of the TMP14 transgenic tobacco is 88%, the aphid inhibition rate of the TMP 14' transgenic tobacco is 2%, and the aphid inhibition rate of the wild tobacco is 2%.
SEQUENCE LISTING
<110> institute of biotechnology of Chinese academy of agricultural sciences
<120> Nicotiana benthamiana TMP14 protein and application thereof in resisting plant viruses
<130> 1111
<160> 4
<170> PatentIn version 3.5
<210> 1
<211> 182
<212> PRT
<213> Nicotiana benthamiana
<400> 1
Met Ala Ser Thr Thr Ser Pro Ser Leu Ser Leu Ser Ser Ser Ser Thr
1 5 10 15
Leu Val Asp Gly Lys Thr Thr Arg Gln Ser Ala Ala Ala Ala Ser Ser
20 25 30
Gln Cys Val Thr Leu Pro Thr Leu Pro Pro Pro Pro Ala Val Gln Ser
35 40 45
Arg Ala Ala Lys Thr Thr Ala Tyr Cys Arg Lys Ile Ala Arg Asn Val
50 55 60
Val Ala Met Ala Thr Ser Thr Gly Glu Val Ala Thr Thr Thr Glu Ala
65 70 75 80
Ser Ser Ala Asp Ile Ala Thr Thr Glu Leu Pro Ala Glu Leu Leu Gln
85 90 95
Lys Ile Gln Glu Ala Trp Asp Lys Leu Asp Asp Lys Tyr Ala Val Ser
100 105 110
Ser Leu Gly Val Ala Ala Leu Leu Leu Leu Trp Ser Ser Thr Gly Val
115 120 125
Ile Ser Ala Ile Asp Arg Leu Pro Leu Ile Pro Gly Val Leu Glu Leu
130 135 140
Val Gly Ile Gly Tyr Thr Gly Trp Phe Ala Tyr Lys Asn Leu Val Phe
145 150 155 160
Lys Pro Asp Arg Glu Ala Leu Ile Ser Lys Ile Lys Asp Leu Tyr Lys
165 170 175
Glu Val Ile Gly Ser Ser
180
<210> 2
<211> 549
<212> DNA
<213> Nicotiana benthamiana
<400> 2
atggcctcaa ctacctcccc ttcactttct ctttcctctt catcgacttt ggtcgacggc 60
aagacaactc gtcagtcagc tgctgctgcc tcctcccaat gtgtcaccct acccaccctc 120
cctcctccac ctgccgtcca aagccgtgct gccaaaacca ctgcgtactg tcgtaagatt 180
gcaagaaatg tggtcgcaat ggcaacgtct actggagagg ttgcaactac tacagaggcc 240
tcatcagctg atatagcaac cactgagcta ccagctgagc ttctccaaaa aattcaagaa 300
gcttgggaca aacttgacga taagtacgca gtcagctcac tcggtgttgc tgcactactt 360
ctactatgga gctccactgg agttatctcg gcaattgaca ggcttcctct gattcctggt 420
gttcttgagc ttgtaggaat tggttacacc ggttggtttg cttacaagaa cttggtcttc 480
aaaccagaca gagaagcttt gatatcaaag atcaaagact tgtacaagga agtaattggg 540
agcagctga 549
<210> 3
<211> 182
<212> PRT
<213> Nicotiana benthamiana
<400> 3
Met Ala Ser Thr Thr Ser Pro Ser Pro Pro Leu Ser Ser Ser Ser Thr
1 5 10 15
Leu Val Asp Gly Lys Thr Thr Arg Gln Ser Ala Ala Ala Ala Ser Ser
20 25 30
Gln Cys Val Thr Leu Pro Thr Leu Pro Pro Pro Pro Ala Val Gln Ser
35 40 45
Arg Ala Ala Lys Thr Thr Ala Tyr Cys Arg Lys Ile Ala Arg Asn Val
50 55 60
Val Ala Met Ala Thr Ser Thr Gly Glu Val Ala Thr Thr Thr Glu Ala
65 70 75 80
Ser Ser Ala Asp Ile Ala Thr Thr Glu Leu Pro Ala Glu Leu Leu Gln
85 90 95
Lys Ile Gln Glu Ala Trp Asp Lys Leu Asp Asp Lys Tyr Ala Val Ser
100 105 110
Ser Leu Gly Val Ala Ala Leu Leu Leu Leu Trp Ser Ser Thr Gly Val
115 120 125
Ile Ser Ala Ile Asp Arg Leu Pro Leu Ile Pro Gly Val Leu Glu Leu
130 135 140
Val Gly Ile Gly Tyr Thr Gly Trp Phe Ala Tyr Lys Asn Leu Val Phe
145 150 155 160
Lys Pro Asp Arg Glu Ala Leu Ile Ser Lys Ile Lys Asp Leu Tyr Lys
165 170 175
Glu Val Ile Gly Ser Ser
180
<210> 4
<211> 549
<212> DNA
<213> Nicotiana benthamiana
<400> 4
atggcctcaa ctacctcccc ttcacctcct ctttcctctt catcgacttt ggtcgacggc 60
aagacaactc gtcagtcagc tgctgctgcc tcctcccaat gtgtcaccct acccaccctc 120
cctcctccac ctgccgtcca aagccgtgct gccaaaacca ctgcgtactg tcgtaagatt 180
gcaagaaatg tggtcgcaat ggcaacgtct actggagagg ttgcaactac tacagaggcc 240
tcatcagctg atatagcaac cactgagcta ccagctgagc ttctccaaaa aattcaagaa 300
gcttgggaca aacttgacga taagtacgca gtcagctcac tcggtgttgc tgcactactt 360
ctactatgga gctccactgg agttatctcg gcaattgaca ggcttcctct gattcctggt 420
gttcttgagc ttgtaggaat tggttacacc ggttggtttg cttacaagaa cttggtcttc 480
aaaccagaca gagaagcttt gatatcaaag atcaaagact tgtacaagga agtaattggg 540
agcagctga 549
Claims (7)
1. The Nicotiana benthamiana TMP14 protein is characterized in that the amino acid sequence of the Nicotiana benthamiana TMP14 protein is shown as a sequence table SEQ ID NO. 1;
wherein, the Nicotiana benthamiana TMP14 protein directly interacts with tomato spotted wilt virus NSm protein.
2. The coding gene of the Bt-TMP 14 protein of claim 1, wherein the nucleotide sequence is represented by SEQ ID NO.2 of the sequence Listing.
3. A vector comprising the gene encoding the Bt TMP14 protein of claim 1.
4. A host bacterium comprising the vector of claim 3.
5. The mutant of the Byssocyanus benthamiana TMP14 protein is characterized in that the amino acid sequence of the Byssocyanus benthamiana TMP14 protein mutant is shown in a sequence table SEQ ID NO. 3.
6. The coding gene of the mutant of Bt TMP14 protein of claim 5, wherein the nucleotide sequence is represented by SEQ ID NO.4 of the sequence Listing.
7. Use of the Nicotiana benthamiana TMP14 protein of claim 1 or the Nicotiana benthamiana TMP14 protein mutant of claim 5 for increasing resistance of tobacco to tomato spotted wilt virus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710667567.8A CN107663231B (en) | 2017-08-07 | 2017-08-07 | Nicotiana benthamiana TMP14 protein and application thereof in resisting plant viruses |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710667567.8A CN107663231B (en) | 2017-08-07 | 2017-08-07 | Nicotiana benthamiana TMP14 protein and application thereof in resisting plant viruses |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107663231A CN107663231A (en) | 2018-02-06 |
| CN107663231B true CN107663231B (en) | 2020-11-27 |
Family
ID=61098271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710667567.8A Active CN107663231B (en) | 2017-08-07 | 2017-08-07 | Nicotiana benthamiana TMP14 protein and application thereof in resisting plant viruses |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107663231B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110499390B (en) * | 2019-09-29 | 2023-03-10 | 云南省烟草农业科学研究院 | Molecular marker primer for tobacco anti-spotted wilt RTSW gene auxiliary selection, auxiliary selection method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194431A (en) * | 2013-04-17 | 2013-07-10 | 北京大学 | Application of RING1 protein for improving RDV (rice dwarf virus) resistance of plants |
| CN109295024A (en) * | 2017-07-24 | 2019-02-01 | 北京大学 | It reduces OsSAMS1 albumen and its encoding gene expression is improving plant to the application in fractilinea oryzae resistance |
-
2017
- 2017-08-07 CN CN201710667567.8A patent/CN107663231B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194431A (en) * | 2013-04-17 | 2013-07-10 | 北京大学 | Application of RING1 protein for improving RDV (rice dwarf virus) resistance of plants |
| CN109295024A (en) * | 2017-07-24 | 2019-02-01 | 北京大学 | It reduces OsSAMS1 albumen and its encoding gene expression is improving plant to the application in fractilinea oryzae resistance |
Non-Patent Citations (6)
| Title |
|---|
| Anastassia Khrouchtchova等.A previously found thylakoid membrane protein of 14kDa (TMP14) is a novel subunit of plant photosystem I and is designated PSI-P.《FEBS Letters》.2005,第579卷(第21期),第4808-4812页. * |
| Ignacio Luque.CURT1,CAAD-containing aaRSs, thylakoid curvature and gene translation.《Trends in Plant Science》.2014,第19卷(第2期),第63-66页. * |
| Unknown.Nicotiana sylvestris protein CURVATURE THYLAKOID 1B, chloroplastic (LOC104223406), mRNA.《GenBank: XM_009774839.1》.2014,ORIGIN部分. * |
| Unknown.protein CURVATURE THYLAKOID 1B, chloroplastic [Nicotiana sylvestris].《GenBank: XP_009773141.1》.2014,ORIGIN部分. * |
| Ute Armbruster.Arabidopsis CURVATURE THYLAKOID1 proteins modify thylakoid architecture by inducing membrane curvature.《Plant Cell》.2013,第25卷(第7期),第2661-2678. * |
| 郑璐平.拟南芥柯浩体蛋白(Atcoilin)的功能预测及亚细胞定位.《农业生物技术学报》.2013,第21卷(第11期),第1270-1278页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107663231A (en) | 2018-02-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1625199B1 (en) | Methods of increasing abiotic stress tolerance and/or biomass in plants and plants generated thereby | |
| EP1941045B1 (en) | Use of a nucleic acid sequence for the generation of a transgenic plant having enhanced drought tolerance | |
| AU2019276382B2 (en) | Use of Yr4DS gene of Aegilops tauschii in stripe rust resistance breeding of Triticeae plants | |
| CN110713526B (en) | Wheat stress-resistant protein TaBZR2D and coding gene and application thereof | |
| CN113215127A (en) | Method for cultivating broad-spectrum disease-resistant TaWRK2A gene-transferred wheat and related biological material thereof | |
| JPH09507124A (en) | Plant pathogenicity gene and its use | |
| CN114276429B (en) | Method for cultivating TaLRK-R gene-transferred wheat with resistance to sheath blight and stem base rot and related biological material thereof | |
| CN110713994B (en) | Plant stress tolerance associated protein TaMAPK3, and coding gene and application thereof | |
| US20150128304A1 (en) | Plant Body Showing Improved Resistance Against Environmental Stress and Method for Producing Same | |
| CN111574606B (en) | Wheat disease-resistant and heading regulation gene TaCOK and related biological material and application thereof | |
| CN107663231B (en) | Nicotiana benthamiana TMP14 protein and application thereof in resisting plant viruses | |
| KR102000454B1 (en) | Promoter recognition site by Xanthomonas oryzae pv. oryzae and uses thereof | |
| CN111574604B (en) | Wheat disease-resistant protein TaAFRK and related biological material and application thereof | |
| US10221427B2 (en) | Gene capable of enhancing salicylic acid-induced cell death in a plant cell and contributing to resistance to the fungal virulence factor deoxynivalenol, resistance to fusarium fungi and fusarium head blight disease, and a recombinant construct including the gene | |
| US10526611B2 (en) | Gene targeting using mutant Agrobacterium strains | |
| US7910801B2 (en) | Plants with reduced expression of phosphatase type 2C gene for enhanced pathogen resistance | |
| US7659447B2 (en) | Increasing host plant susceptibility to agrobacterium infection by overexpression of the arabidopsis VIP1 gene | |
| CN109593782B (en) | Disease-resistant plant obtained by using HiR3s gene of Nicotiana benthamiana and application of gene | |
| CN110684114B (en) | Application of plant stress tolerance associated protein TaBAKL in regulation and control of plant stress tolerance | |
| CN107177602B (en) | NtDR1 gene related to drought tolerance of plant and application thereof | |
| WO1999054490A2 (en) | Plant-derived resistance gene | |
| CN110194791B (en) | Application of SPL3 protein in regulation and control of plant inflorescence or carpopodium development | |
| CN112143737B (en) | Application of OsbZIP62-VP64 fusion expression in improving agronomic traits of rice | |
| CN111205355B (en) | Plant stress tolerance related protein SiWRKY76 and coding gene and application thereof | |
| CN111285927B (en) | Plant stress tolerance related protein SiWRKY78 and coding gene and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |