CN107663231A - Ben Shi cigarette TMP14 albumen and its application in Antiphytoviral - Google Patents

Ben Shi cigarette TMP14 albumen and its application in Antiphytoviral Download PDF

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Publication number
CN107663231A
CN107663231A CN201710667567.8A CN201710667567A CN107663231A CN 107663231 A CN107663231 A CN 107663231A CN 201710667567 A CN201710667567 A CN 201710667567A CN 107663231 A CN107663231 A CN 107663231A
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tmp14
ben shi
albumen
shi cigarette
cigarette
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CN107663231B (en
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张永强
张超
占锦
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Biotechnology Research Institute of CAAS
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

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  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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  • Peptides Or Proteins (AREA)

Abstract

Application the invention discloses Ben Shi cigarette TMP14 albumen and its in Antiphytoviral.The amino acid sequence of the Ben Shi cigarette TMP14 albumen is as shown in sequence table SEQ ID NO1.The invention also discloses one of Ben Shi cigarette TMP14 albumen to optimize mutant.Direct interaction be present with TSWV NSm in the Ben Shi cigarette TMP14 albumen of the present invention, it, which is overexpressed transgenic tobacco plant, has the function of Antiphytoviral, the discovery provides theoretical and practical basis for the research antiviral crops of transgenosis, has broad application prospects.

Description

Ben Shi cigarette TMP14 albumen and its application in Antiphytoviral
Technical field
The invention belongs to technical field of molecular biology, and in particular to a kind of Ben Shi cigarette TMP14 albumen and its in anti-plant Application in virus.
Background technology
TMP14 is a kind of phosphorylated protein (thylakoid membrane for being positioned at Thylakoid membrane phosphoprotein,TMP).The albumen is by nuclear gene encoding, precursor bag to be shown to arabidopsis TMP14 (AtTMP14) researchs Chloroplast signal peptide containing 45 amino acid compositions.AtTMP14 molecular weight is 14kDa, thus it is speculated that N-terminal is located at chloroplaset base Matter, intermediate region include 2 transbilayer helixs, and the threonine of 21 or 22 is phosphorylation site.TMP14 is the light such as plant, blue-green algae A kind of peculiar albumen of symphysis thing coding, but the concrete function in photosynthesis does not understand still so far.It recent studies have shown that, TMP14 is one of composition subunit of plant photosystem-I (photosystem I, PSI) pigment-protein complexes, therefore is named again For PSI-P, thus it is speculated that it plays an important roll in plant light reaction.NbTMP14 is Ben Shi cigarette chloroplaset photosystem-I composition Subunit, full length gene 549nt, the precursor protein of coding include the chloroplast signal peptide of 20 amino acid compositions.
Tomato spotted wilf virus (Tomato spotted wilt virus, TSWV) is bunyaviridae (Bunyaviridae) representative species of Tospovirus (Tospovirus), is propagated by Frankliniella occidentalis, infects host 1000 Remaining kind, trigger the classical symptoms such as chlorisis, necrosis, ring spot in organs such as the blade of plant, stalk, fruits, seriously endanger global agriculture Industry produces.NSm is the distinctive encoding proteins of Tospovirus viruses, and NSm is in Tospovirus viruses and host plant interaction mistake There is very important effect in journey.Found using being studied the methods of biochemistry, histochemistry TSWV NSm, NSm has The characteristic feature of motor protein (movement protein, MP), such as：1) it is close with cytoplasma membrane, cell wall-bound, 2) RNA Affine, 3) it is positioned at plasmodesmus, 4) modification plasmodesmus.At present, still not on TMP14 albumen and TSWV NSm albumen Interaction, and the report of plant virus is resisted, the effect of Ben Shi cigarette TMP14 albumen is also unknown.
The content of the invention
It is an object of the invention to provide a kind of Ben Shi cigarette TMP14 albumen and its encoding gene.
The present invention also aims to provide Ben Shi cigarette TMP14 albumen with TSWV NSm interactions between protein and its in anti-phytopathy Application in poison.
A kind of Ben Shi cigarette TMP14 albumen, the amino acid sequence such as sequence table SEQ ID of the Ben Shi cigarette TMP14 albumen Shown in NO1.
The gene order of the Ben Shi cigarette TMP14 albumen is as shown in sequence table SEQ ID NO2.
The genophore of above-mentioned Ben Shi cigarette TMP14 albumen.
The Host Strains of the genophore of above-mentioned Ben Shi cigarette TMP14 albumen.
Expand the primer of any fragment in above-mentioned Ben Shi cigarette TMP14 genes.
A kind of Ben Shi cigarette TMP14 protein mutants, the amino acid sequence such as sequence of the Ben Shi cigarette TMP14 protein mutants Shown in table SEQ ID NO3.
Above-mentioned Ben Shi cigarette TMP14 protein mutants, its gene order is as shown in sequence table SEQ ID NO4.
The interaction of above-mentioned Ben Shi cigarette TMP14 albumen and TSWV NSm albumen.
The application of above-mentioned Ben Shi cigarette TMP14 albumen and Ben Shi cigarette TMP14 protein mutants in plant virus is resisted.
Beneficial effects of the present invention：Direct interaction be present with TSWV NSm in the Ben Shi cigarette TMP14 albumen of the present invention；It crosses table There is the function of Antiphytoviral up to transgenic tobacco plant, the discovery provides reason for the research antiviral crops of transgenosis Practical basis is referred to, is had broad application prospects；Its mutain has more excellent Antiphytoviral effect.
Brief description of the drawings
Fig. 1 is the yeast two-hybrid figure of Ben Shi cigarette TMP14 albumen and TSWV NSm interactions between protein.
Double fluorescence complementary method validation Ben Shi cigarette TMP14 and TSWV the NSm interactions of Fig. 2.
Fig. 3 Ben Shi cigarettes are inoculated with TSWV and VIGS and lower TMP14 Symptoms；
In figure, A.TSWV be inoculated with 10 days after compared with healthy plant；B.TRV VIGS method silence TMP14 Symptoms； C. be inoculated with TSWV after 10 days qPCR detect TMP14 expression quantity；D.qPCR detects TMP14 silencing efficiencies.
Fig. 4 is that electron microscope observation is inoculated with TSWV and lowers Ben Shi cigarette chloroplaset form after TMP14 expression.
The Ben Shi cigarette plant inoculation TSWV Symptoms and the detection of viral accumulation that Fig. 5 TMP14 expression is lowered.
Fig. 6 turns the identification of TMP14 genes Ben Shi cigarette and TSWV challenge tests；
In figure, Symptoms after A. is inoculated with TSWV10 days；B.qPCR analyzes TMP14 expression quantity；C.TSWV accumulations are examined Survey；D.Western Blot detect transfer-gen plant.
Embodiment
The present invention will be further described with specific embodiment below in conjunction with the accompanying drawings.
Direct interaction be present in the TSWV NSm of embodiment 1 and Ben Shi cigarette TMP14
TSWV NSm are inserted into pBT respectively3- STE, NbTMP14 are inserted into pPR3- N, obtain pBT3- STE-NSm with pPR3Two carriers of-N-TMP14.Both carriers are transferred in yeast strain NMY51 simultaneously, while with pBT3-STE-NSm With empty carrier pPR3- N, with pPR3- N-TMP14 and empty carrier pBT3- STE cotransformations yeast applies ferment as two groups of negative controls Mother two lacks plate, then identifies positive colony by bacterium solution PCR.Thalline is collected after positive colony is shaken into bacterium, is diluted to various concentrations, Further apply yeast three and lack plate, observation three lacks the growing way of plate.As a result pBT is shown3- STE-NSm and pPR3- N-TMP14 cotransformations Yeast can lack normal growth on plates, and pBT three3- STE-NSm and empty carrier pPR3-N、pPR3- N-TMP14 and empty carrier pBT3The yeast of-STE cotransformations is then suppressed, and illustrates that TSWV NSm and Ben Shi cigarette TMP14 has direct interaction (Fig. 1).
NbTMP14 and TSWV NSm are inserted respectively into bimolecular fluorescence carrier YFPN and YFPC, obtain TMP14- Two carriers of YFPN and NSm-YFPC.TMP14-YFPN and NSm-YFPC carriers are transformed into Agrobacterium GV3101.Utilize agriculture The mode that bacillus is infected, respectively to being seen when Ben Shi cigarette co-injection TMP14-YFPN and NSm-YFPC Agrobacterium, 36h-48h or so Examine TMP14 positioning.It was found that in wild type Ben Shi cigarette, there is yellow fluorescence protein, and be positioned on chloroplaset, this is also further TMP14 and NSm interaction are demonstrated, and the position of interaction occurs in chloroplaset (Fig. 2).
Result above proves that Ben Shi cigarette TMP14 albumen has direct interaction with TSWV NSm.NSm is the one of TSWV codings The important multifunctional protein of kind.Mediate retroviral completes transcellular movement, and related to symptom generation.TMP14 therewith interaction in virus The effect played in infection processs needs further to be studied.
The TSWV of embodiment 2 inoculations Ben Shi cigarette causes the lower Development of Chloroplasts that reconciles of TMP14 expression abnormal
Ben Shi cigarette frictional inoculation virus TSWV, tobacco pathogenesis symptom is observed, after inoculation 10 days, Ben Shi cigarette system blade goes out Now obvious yellow wilting symptom.Incidence of leaf is taken to extract total serum IgE, after RT-PCR confirms virus infection, qPCR detection TMP14 tables Up to amount, find TMP14 expression quantity substantially reduces (A in Fig. 3, C) compared with health tobacco.
Incidence of leaf is taken utilize electron microscope observation eucaryotic cell structure simultaneously, the chloroplaset of tobacco is bright after discovery virus inoculation It is aobvious bigger than the amylum body on the chloroplaset of wild-type tobacco, and the structure of chloroplaset is destroyed, and form generation is bright Aobvious change (Fig. 4).
In order to further determine that virus symptoms occur and TMP14 expression is lowered relation, using TRV VIGS mode, Expressed downward.Two carriers of pTRV2-GFP, pTRV2-TMP14 are built respectively；By converting agriculture bar GV3101, pTRV2- GFP, pTRV2-TMP14 respectively with TRV1 1:Tobacco is injected after 1 mixing；10 days phenotypes for obvious yellow occur, qPCR after injection Detect Ben Shi cigarette TMP14 expression quantity and decline (B in Fig. 3, D), using the form of electron microscopic observation chloroplaset, find silence TMP14 Tobacco chloroplast compared with the form of chloroplaset of control Ben Shi cigarette by heavy damage (Fig. 4).
The downward Ben Shi cigarette TMP14 expression of embodiment 3 is advantageous to TSWV and infected, and up-regulation then improves to its resistance
TSWV accumulations are caused to raise after 1.TRV VIGS silences TMP14 expression
TRV2-GFP, TRV2-TMP14 respectively with TRV1 1:Tobacco is injected after 1 mixing；10 days after injection, sampling detects this Two expression quantity for growing tobacco middle TMP14, as a result find the expression quantity of TMP14 in TRV2-TMP14 substantially than in TRV2-GFP TMP14 expression quantity is low.It is viral that TSWV is further inoculated with to pTRV2-GFP, pTRV2-TMP14 tobacco, 10 after virus inoculation My god, sampling detects the accumulation of TSWV in both tobaccos, find in pTRV2-TMP14 tobaccos viral accumulation substantially than Viral accumulation will height (Fig. 5) in pTRV2-GFP tobaccos.
2. transgenosis is overexpressed TMP14 Ben Shi cigarettes and TSWV resistances is improved
Gene fusion construct TMP14-GFP, TMP14-GFP fusions are inserted into PBI121 carriers, obtain carrier TMP14-GFP-PBI121；Agrobacterium LBA4404 is converted again, obtains strain.Finally tested using genetic transformation by TMP14- This carriers of GFP-PBI121 conversion tissue culture tobacco, induced by callus, the screening of kanamycin-resistant callus tissue, the differentiation of kanamycin-resistant callus tissue and Root induction, finally give TMP14-GFP-PBI121 transgene tobacco seedling TMP14-OE.PCR, protein spots are utilized simultaneously Miscellaneous and Western is tested to identify that the transgene tobacco is positive plant.QPCR detects the transgene tobacco and contemporaneity TMP14 expression quantity in wild-type tobacco, find the expression quantity of TMP14 in the transgene tobacco substantially than in wild-type tobacco TMP14 expression quantity is high.Tobacco further is turned to this and wild-type tobacco inoculation TSWV is viral, 10 days after virus inoculation, TMP14-OE plant non-evident sympton produces, and obvious leaf rolling occurs in wild type Ben Shi cigarette.Sampling detects both cigarettes TSWV accumulation in grass, find accumulation viral in the transgene tobacco substantially accumulation more viral than in wild-type tobacco Measure low (Fig. 6).
Result above proves that Ben Shi cigarette TMP14 plays a significant role during virus infection, and virus infection causes its table Up to downward, and then chloroplaset normal development is influenceed, form serious virus symptoms.When being overexpressed in Ben Shi cigarette body, It is unfavorable for TSWV virus accumulation, resistance is produced to it.Analyze its reason, be on the one hand overexpressed TMP14 make its largely with NSm interactions With reference to disturbing its normal function；Being overexpressed TMP14 in addition helps to maintain chloroplaset normal morphology, prevents symptom.
Embodiment 4
It is masterplate using TMP14-GFP-PBI121 plant expression vectors, is mutated by the method for Overlap extension PCR Body, mutational site are that the 9th and the 10th amino acids residue LS sports PP.Rebuild plant expression vector TMP14 '-GFP- PBI121, and by Agrobacterium-mediated transformation tobacco, obtain the transgene tobacco of the TMP14 ' genes of 35S promoter driving Plant.
Embodiment 5
Plantation turns tobacco and the wild-type tobacco of TMP14 genes, and tobacco growing was by 60 days, when seedling stage will terminate, Take and grow in the same size, the consistent tobacco plant of lobe numbers, inoculation TSWV is viral, and after virus inoculation after 10 days, statistics produces The plant number (produce obvious patch more than 3 be recorded as Infective) of viral plaque.
Experimental result：The infection rate for turning the tobacco of TMP14 genes is 38%, and the infection rate of wild-type tobacco is 99%.
Embodiment 6
Plantation turns tobacco and the wild-type tobacco of TMP14 ' genes, and tobacco growing was by 60 days, when seedling stage will terminate, Take and grow in the same size, the consistent tobacco plant of lobe numbers, inoculation TSWV is viral, and after virus inoculation after 10 days, statistics produces The plant number (produce obvious patch more than 3 be recorded as Infective) of viral plaque.
Experimental result：The infection rate for turning the tobacco of TMP14 genes is 2%, and the infection rate of wild-type tobacco is 99%.
The aphids resistance of embodiment 7 (Semiptera) is tested
Plantation turns TMP14 genes and turns tobacco and the wild-type tobacco of TMP14 ' genes, and tobacco growing was by 60 days, seedling When phase will terminate, take and grow in the same size, the consistent tobacco plant of lobe numbers, turn the tobacco plant 30 of PtTI genes Strain, 30 plants of wild-type tobacco, 100 aptery aphids of maturation are connect on every plant of tobacco, after 30 hours, count aphid inhibiting rate：Aphid Worm inhibiting rate %=(remaining aphid on 100- blades)/100.
Experimental result：The aphid inhibiting rate for turning the tobacco of TMP14 genes is 88%, turns the aphid of the tobacco of TMP14 ' genes Inhibiting rate is 2%, and the aphid inhibiting rate of wild-type tobacco is 2%.
SEQUENCE LISTING
<110>Biological Technology institute, Chinese Academy of Agricultural Sciences
<120>Ben Shi cigarette TMP14 albumen and its application in Antiphytoviral
<130> 1111
<160> 4
<170> PatentIn version 3.5
<210> 1
<211> 182
<212> PRT
<213> Nicotiana benthamiana
<400> 1
Met Ala Ser Thr Thr Ser Pro Ser Leu Ser Leu Ser Ser Ser Ser Thr
1 5 10 15
Leu Val Asp Gly Lys Thr Thr Arg Gln Ser Ala Ala Ala Ala Ser Ser
20 25 30
Gln Cys Val Thr Leu Pro Thr Leu Pro Pro Pro Pro Ala Val Gln Ser
35 40 45
Arg Ala Ala Lys Thr Thr Ala Tyr Cys Arg Lys Ile Ala Arg Asn Val
50 55 60
Val Ala Met Ala Thr Ser Thr Gly Glu Val Ala Thr Thr Thr Glu Ala
65 70 75 80
Ser Ser Ala Asp Ile Ala Thr Thr Glu Leu Pro Ala Glu Leu Leu Gln
85 90 95
Lys Ile Gln Glu Ala Trp Asp Lys Leu Asp Asp Lys Tyr Ala Val Ser
100 105 110
Ser Leu Gly Val Ala Ala Leu Leu Leu Leu Trp Ser Ser Thr Gly Val
115 120 125
Ile Ser Ala Ile Asp Arg Leu Pro Leu Ile Pro Gly Val Leu Glu Leu
130 135 140
Val Gly Ile Gly Tyr Thr Gly Trp Phe Ala Tyr Lys Asn Leu Val Phe
145 150 155 160
Lys Pro Asp Arg Glu Ala Leu Ile Ser Lys Ile Lys Asp Leu Tyr Lys
165 170 175
Glu Val Ile Gly Ser Ser
180
<210> 2
<211> 549
<212> DNA
<213> Nicotiana benthamiana
<400> 2
atggcctcaa ctacctcccc ttcactttct ctttcctctt catcgacttt ggtcgacggc 60
aagacaactc gtcagtcagc tgctgctgcc tcctcccaat gtgtcaccct acccaccctc 120
cctcctccac ctgccgtcca aagccgtgct gccaaaacca ctgcgtactg tcgtaagatt 180
gcaagaaatg tggtcgcaat ggcaacgtct actggagagg ttgcaactac tacagaggcc 240
tcatcagctg atatagcaac cactgagcta ccagctgagc ttctccaaaa aattcaagaa 300
gcttgggaca aacttgacga taagtacgca gtcagctcac tcggtgttgc tgcactactt 360
ctactatgga gctccactgg agttatctcg gcaattgaca ggcttcctct gattcctggt 420
gttcttgagc ttgtaggaat tggttacacc ggttggtttg cttacaagaa cttggtcttc 480
aaaccagaca gagaagcttt gatatcaaag atcaaagact tgtacaagga agtaattggg 540
agcagctga 549
<210> 3
<211> 182
<212> PRT
<213> Nicotiana benthamiana
<400> 3
Met Ala Ser Thr Thr Ser Pro Ser Pro Pro Leu Ser Ser Ser Ser Thr
1 5 10 15
Leu Val Asp Gly Lys Thr Thr Arg Gln Ser Ala Ala Ala Ala Ser Ser
20 25 30
Gln Cys Val Thr Leu Pro Thr Leu Pro Pro Pro Pro Ala Val Gln Ser
35 40 45
Arg Ala Ala Lys Thr Thr Ala Tyr Cys Arg Lys Ile Ala Arg Asn Val
50 55 60
Val Ala Met Ala Thr Ser Thr Gly Glu Val Ala Thr Thr Thr Glu Ala
65 70 75 80
Ser Ser Ala Asp Ile Ala Thr Thr Glu Leu Pro Ala Glu Leu Leu Gln
85 90 95
Lys Ile Gln Glu Ala Trp Asp Lys Leu Asp Asp Lys Tyr Ala Val Ser
100 105 110
Ser Leu Gly Val Ala Ala Leu Leu Leu Leu Trp Ser Ser Thr Gly Val
115 120 125
Ile Ser Ala Ile Asp Arg Leu Pro Leu Ile Pro Gly Val Leu Glu Leu
130 135 140
Val Gly Ile Gly Tyr Thr Gly Trp Phe Ala Tyr Lys Asn Leu Val Phe
145 150 155 160
Lys Pro Asp Arg Glu Ala Leu Ile Ser Lys Ile Lys Asp Leu Tyr Lys
165 170 175
Glu Val Ile Gly Ser Ser
180
<210> 4
<211> 549
<212> DNA
<213> Nicotiana benthamiana
<400> 4
atggcctcaa ctacctcccc ttcacctcct ctttcctctt catcgacttt ggtcgacggc 60
aagacaactc gtcagtcagc tgctgctgcc tcctcccaat gtgtcaccct acccaccctc 120
cctcctccac ctgccgtcca aagccgtgct gccaaaacca ctgcgtactg tcgtaagatt 180
gcaagaaatg tggtcgcaat ggcaacgtct actggagagg ttgcaactac tacagaggcc 240
tcatcagctg atatagcaac cactgagcta ccagctgagc ttctccaaaa aattcaagaa 300
gcttgggaca aacttgacga taagtacgca gtcagctcac tcggtgttgc tgcactactt 360
ctactatgga gctccactgg agttatctcg gcaattgaca ggcttcctct gattcctggt 420
gttcttgagc ttgtaggaat tggttacacc ggttggtttg cttacaagaa cttggtcttc 480
aaaccagaca gagaagcttt gatatcaaag atcaaagact tgtacaagga agtaattggg 540
agcagctga 549

Claims (9)

  1. A kind of 1. Ben Shi cigarette TMP14 albumen, it is characterised in that the amino acid sequence such as sequence table of the Ben Shi cigarette TMP14 albumen Shown in SEQ ID NO1.
  2. 2. Ben Shi cigarette TMP14 albumen according to claim 1, it is characterised in that the gene sequence of the Ben Shi cigarette TMP14 albumen Row are as shown in sequence table SEQ ID NO2.
  3. A kind of 3. genophore containing Ben Shi cigarette TMP14 albumen described in claim 1.
  4. 4. the Host Strains containing the genophore of Ben Shi cigarette TMP14 albumen described in claim 3.
  5. 5. expand the primer of any fragment in the Ben Shi cigarette TMP14 genes described in claim 1.
  6. A kind of 6. Ben Shi cigarette TMP14 protein mutants, it is characterised in that the amino acid of the Ben Shi cigarette TMP14 protein mutants Sequence is as shown in sequence table SEQ ID NO3.
  7. 7. Ben Shi cigarette TMP14 protein mutants according to claim 6, it is characterised in that the Ben Shi cigarette TMP14 albumen is dashed forward The gene order of variant is as shown in sequence table SEQ ID NO4.
  8. 8. the interaction of Ben Shi cigarette TMP14 albumen described in claim 1 and TSWV NSm albumen.
  9. 9. Ben Shi cigarette TMP14 protein mutants described in Ben Shi cigarette TMP14 albumen described in claim 1 and claim 6 are being resisted Application in plant virus.
CN201710667567.8A 2017-08-07 2017-08-07 Nicotiana benthamiana TMP14 protein and application thereof in resisting plant viruses Active CN107663231B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110499390A (en) * 2019-09-29 2019-11-26 云南省烟草农业科学研究院 Molecular labeling primer, the method and its application of assisted Selection for the anti-spotted wilt RTSW gene-assisted selection of tobacco

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103194431B (en) * 2013-04-17 2014-07-09 北京大学 Application of RING1 protein for improving RDV (rice dwarf virus) resistance of plants
CN109295024B (en) * 2017-07-24 2022-02-08 北京大学 Application of OsSAMS1 protein and coding gene expression thereof in improving resistance of plants to rice dwarf virus

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110499390A (en) * 2019-09-29 2019-11-26 云南省烟草农业科学研究院 Molecular labeling primer, the method and its application of assisted Selection for the anti-spotted wilt RTSW gene-assisted selection of tobacco
CN110499390B (en) * 2019-09-29 2023-03-10 云南省烟草农业科学研究院 Molecular marker primer for tobacco anti-spotted wilt RTSW gene auxiliary selection, auxiliary selection method and application thereof

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