CN107660652A - The preparation method of prawn breeding period fortified feed - Google Patents

The preparation method of prawn breeding period fortified feed Download PDF

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Publication number
CN107660652A
CN107660652A CN201710732794.4A CN201710732794A CN107660652A CN 107660652 A CN107660652 A CN 107660652A CN 201710732794 A CN201710732794 A CN 201710732794A CN 107660652 A CN107660652 A CN 107660652A
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parts
prawn
preparation
breeding period
feed
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陈义娜
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Pujiang County Cooperative Animal Feed Technology Development Co Ltd
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Pujiang County Cooperative Animal Feed Technology Development Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/24—Animal feeding-stuffs from material of animal origin from blood
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Abstract

The invention discloses a kind of preparation method of prawn breeding period fortified feed, including：Fermented bean dregs, the preparation of tea polyphenol liposomes, compounding.Have the beneficial effect that：The ANFs in dregs of beans after fermentation is decomposed removal, rich in protein, organic matter, middle trace element, is suitable as the base-material of prawn feed；Microorganism can change microbiologic population's composition of gut of shrimp after entering in prawn body, improve the prawn speed of growth, enhancing resistance against diseases, improve immunocompetence；Rich in nutriments such as protein, amino acid, fat, vitamin, mineral elements in feed, it is aided with phagostimulant, the nutritional requirement needed for close shrimp growth and development can be met, promote prawn growth and gonad development, be ready for its breeding, be it is a kind of be efficiently directed to, the nutrient enrichment fodder of the prawn breeding period of safe and non-toxic secondary residual.

Description

The preparation method of prawn breeding period fortified feed
Technical field
The invention belongs to aquatic livestock fortified feed field, more particularly to a kind of preparation side of prawn breeding period fortified feed Method.
Technical background
Prawn, scientific name oriental prawn, also known as Chinese prawn, Crustin, spot section shrimp.Arthropoda, hapalonychia guiding principle, ten Sufficient mesh, Penaeidae, Penaeus.Penaeus individual is big, is generally called prawn.General 16~22 lis of the female growing individual body length of prawn Rice, 50~80 grams are weighed about, maximum is reachable 30 centimetres, weighs 250 grams；Male is smaller, and body grows 13~18 centimetres, weighs 30~50 grams. Prawn is wide warm eurysalinity aquatic/marine animals.Mang oxen island prawn body is in long tubular, and left and right is flat-sided, and body is divided into head, chest and belly, by 20 body segment compositions.Belly is longer, muscular, and merogenesis is obvious.Shrimp is nutritious, and its meat is soft, easy to digest, to body People that is weak and needing to raise after being ill is fabulous food, contains abundant magnesium in shrimp, magnesium has important to cardiomotility Adjustment effect, cardiovascular system can be protected well, it can reduce blood cholesterol level, prevent artery sclerosis, also simultaneously Energy coronary artery dilator, is advantageous to preventing hypertension and myocardial infarction；The lactation promoting effect of shrimp is stronger, and is rich in phosphorus, calcium, to small Youngster, pregnant woman have help effect especially；A kind of critically important material is exactly astaxanthin in prawn body, is exactly the composition of surface red color, Astaxanthin is the most strong a kind of antioxidant having now been found that, color is deeper, and explanation content astaxanthin is higher.It is widely used in cosmetic Product, food addition and medicine.
Technology of preparing on prawn feed has many methods, and as applied, public number is the B of CN 102106478 to prior art Chinese invention patent, disclose a kind of prawn feed, in the prawn feed of the inventive method containing fish meal, dregs of beans, peanut press pulp, Rapeseed dregs, brewer's yeast, flour, starch, corn oil, fish oil, K2HPO4, Chinese herbaceous peony general flavone, the inventive method prepare Chinese herbaceous peony it is total Petroleum ether extraction is used in the step of flavones, the residual of petroleum ether can be caused so as to produce potential hazard to prawn, and dregs of beans, Contain anti-nutrition component in rapeseed dregs, absorption of the prawn to feed nutrient can be adversely affected, it is main in feed Component fish meal prices are expensive, add the cost of feed.
The content of the invention
The purpose of this method is to provide a kind of preparation method of prawn breeding period fortified feed, and preparation method is simply easily grasped Make, fortified feed nutrition general equilibrium, nutritive value is higher, and can promote the Gonad Differentiation of breeding period prawn, promotes its property Allelotaxis.
Microorganism fungus kind and its bought enterprise used are in the inventive method：Bacillus licheniformis, saccharomycete, lactic acid Bacterium, the industrial Bioisystech Co., Ltd in Cangzhou.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Prawn breeding period fortified feed Preparation method, including：Fermented bean dregs, the preparation of tea polyphenol liposomes, compounding, specifically include following steps：
Fermented bean dregs：Dregs of beans 100-150 parts, molasses 2-3 parts are weighed, it is 40-50% to add distillation water management water content, adjusts pH For 6.8-7.2, temperature is 23-28 DEG C, is inoculated with bacillus licheniformis 2.0-2.5%, saccharomycete 1.8-2.0%, lactic acid bacteria 2.0- 2.2%, ferment 72-96 hours, low temperature dewatering produces fermented bean dregs per 24-36 hours stirring once；Through everfermentation, in dregs of beans ANFs is decomposed removal, rich in protein, organic matter, middle trace element, is suitable as the base-material of prawn feed；Micro- life Thing can change microbiologic population's composition of gut of shrimp after entering in prawn body, improve the prawn speed of growth, the disease-resistant energy of enhancing Power, improve immunocompetence；
The preparation of tea polyphenol liposomes：Weigh the sodium dihydrogen phosphate and disodium hydrogen phosphate that 0.08-0.10mol/L, pH are 7.3-7.5 Mixed solution 400-450 parts, Tea Polyphenols 8.2-8.5 parts are slowly added to, 55-58 DEG C is heated under strong stirring and keeps constant temperature； Egg yolk lecithin 250-280 parts, cholesterol 32-35 parts, VE10-15 parts are weighed, is added in 200-300 part absolute ethyl alcohols, adds Heat adds mixed solution in Tea Polyphenols solution after dissolving completely, keeping temperature strong stirring 50- to 55-58 DEG C of stirring and dissolving 60 minutes, then rotary evaporation 30-35 minutes removed residual ethanol under 45-50 DEG C of temperature, 0.10-0.15MPa vacuum, then Be ultrasonically treated, ultrasonic power 250-280W, frequency is 28-30 KHz, with the ultrasonic 4-5 seconds, the interval 3-4 seconds frequency ultrasound at Manage the 90-100 seconds, tea polyphenol liposomes are placed in 4-6 DEG C of preservation by ultrasound after terminating；Tea polyphenol liposomes encapsulating prepared by the method Rate is high, is easy to the absorption of prawn, and preparation method is simple, is avoided in preparation process containing potentially harmful substances such as ether Physics and chemistry processing, it is especially suitable for industrialization large-scale production；
Compounding：Weigh fermented bean dregs 45-60 parts, congealed fat soya-bean oil 10-12 parts, blood meal 2-3 parts, meat meal tankage 1-2 parts, dusty yeast 3-5 Part, salt 3.0-5.0 parts, calcium dihydrogen phosphate 1.0-1.5 parts, krill meal 5.0-6.0 parts, cuttlefish powder 5.0-6.0 parts, active peptides 0.03-0.04 parts, Choline Chloride 0.3-0.5 parts, taurine 1.0-1.5 parts, vitamin premix 2.0-2.5 parts, mineral matter are pre- Batch mixing 1.0-1.2 parts, calcium propionate 0.05-0.06 parts, ethoxyquin beautiful jade 0.05-0.06 parts, sodium alginate 0.6-0.7 parts, courage are solid The sub-sieve of 80-100 mesh is crossed after alcohol 0.8-1.0 parts, composite feeding promoting agent 1.0-2.0 part co-grindings, particle is sifted out and crushes again Sieving, feed meal injection comminutor wet granulation, particle diameter 2-4mm, it is numerous to produce prawn again with mixer mixing 10-15 minutes Grow the fortified feed of phase；The amino acid sequence of active peptides is：SCASRCKSRCRARRCRARRCGYYYFGKRCICKCFRC；It is living Property polypeptide can stimulate prawn pituitary gonadotropin cell ahead of time develop and enter secretory activity, promote sexual gland divide Change, promote the development of sexual organ；It is auxiliary rich in nutriments such as protein, amino acid, fat, vitamin, mineral elements in feed With phagostimulant, the nutritional requirement needed for close shrimp growth and development can be met, promote prawn growth and gonad development, for its breeding Be ready, be it is a kind of be efficiently directed to, the nutrient enrichment fodder of the prawn breeding period of safe and non-toxic secondary residual.
Preferably, vitamin premix used is VA or VB1Or VB2Or VB6Or VB12Or VC or VD or VE or VK or Calcium pantothenate or at least five kinds of mixture of nicotinic acid or folic acid or biotin or inositol or microcrystalline cellulose；Vitamin premix can To strengthen the antibacterial resistance against diseases of close shrimp, immunity, copingability are improved, reduces its death rate.
Preferably, mineral substance premix used is MgSO4·7H2O and CuSO4·5H2O and FeSO4·H2O with ZnSO4·H2O and MnSO4·H2O and CoCl2·H2O and Ca (IO3)2With Na2SeO3With the mixture of zeolite powder；Mineral matter premixes Trace element in expecting necessary to being grown for close shrimp supplement.
Preferably, composite feeding promoting agent used is glycine betaine or neopentanoic acid thetin or glycine or alanine or 5- At least three kinds of mixture in inosinyl phosphate inosine or dried orange peel or brewex's grains or dried silkworm chrysalis meal；Composite feeding promoting agent can strengthen the suitable of feed Mouth property, so as to promote close shrimp to feed.
Compared with prior art, the advantage of the invention is that：1）The ANFs in dregs of beans after fermentation is decomposed Remove, rich in protein, organic matter, middle trace element, be suitable as the base-material of prawn feed；Microorganism is after entering in prawn body Microbiologic population's composition of gut of shrimp can be changed, the prawn speed of growth, enhancing resistance against diseases is improved, improve immunocompetence；2） Active peptides can stimulate prawn pituitary gonadotropin cell to develop ahead of time and enter secretory activity, promote point of sexual gland Change, promote the development of sexual organ；3）The nutriments such as protein, amino acid, fat, vitamin, mineral element are rich in feed, It is aided with phagostimulant, the nutritional requirement needed for close shrimp growth and development can be met, promote prawn growth and gonad development, is that its is numerous Grow and be ready, be it is a kind of be efficiently directed to, the nutrient enrichment fodder of the prawn breeding period of safe and non-toxic secondary residual.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
The preparation method of prawn breeding period fortified feed, comprises the following steps：
1）100 parts of dregs of beans, 2 parts of molasses are weighed, it is 40% to add distillation water management water content, and regulation pH is 6.8, and temperature is 23 DEG C, Bacillus licheniformis 2.0% is inoculated with, saccharomycete 1.8%, lactic acid bacteria 2.0%, once, fermentation 72 hours, low temperature takes off every 24 hours stirrings Water produces fermented bean dregs；Through everfermentation, the ANFs in dregs of beans is decomposed removal, rich in protein, organic matter, in it is micro Element, it is suitable as the base-material of prawn feed；
2）400 parts of the sodium dihydrogen phosphate and disodium hydrogen phosphate mixed solution that 0.08mol/L, pH are 7.3 are weighed, it is more to be slowly added to tea 8.2 parts of phenol, 55 DEG C are heated under strong stirring and keeps constant temperature；250 parts of egg yolk lecithin, 32 parts of cholesterol, VE10 parts are weighed, It is added in 200 parts of absolute ethyl alcohols, is heated to 55 DEG C of stirring and dissolvings, mixed solution is added into Tea Polyphenols solution after dissolving completely In, keeping temperature strong stirring 50 minutes, then rotary evaporation removes residual in 30 minutes under 45 DEG C of temperature, 0.10MPa vacuum Ethanol, then it is ultrasonically treated, ultrasonic power 250W, frequency 28KHz, with ultrasonic 4 seconds, the interval frequency ultrasound of 3 seconds processing 90 seconds, tea polyphenol liposomes were placed in 4 DEG C of preservations by ultrasound after terminating；
3）Weigh 45 parts of fermented bean dregs, 10 parts of congealed fat soya-bean oil, 2 parts of blood meal, 1 part of meat meal tankage, 3 parts of dusty yeast, 3.0 parts of salt, phosphorus 1.0 parts of acid dihydride calcium, 5.0 parts of krill meal, 5.0 parts of cuttlefish powder, 0.03 part of active peptides, 0.3 part of Choline Chloride, taurine 1.0 Part, 2.0 parts of vitamin premix, 1.0 parts of mineral substance premix, 0.05 part of calcium propionate, 0.05 part of ethoxyquin beautiful jade, sodium alginate 0.6 part, 0.8 part of cholesterol, 0.5 part of glycine betaine, 0.2 part of neopentanoic acid thetin, 80 mesh are crossed after 0.3 part of co-grinding of glycine Sub-sieve, sift out particle and pulverize and sieve again, feed meal again with mixer mix 10 minutes, inject comminutor wet granulation, Particle diameter is 2mm, produces the fortified feed of prawn breeding period；The amino acid sequence of active peptides is： SCASRCKSRCRARRCRARRCGYYYFGKRCICKCFRC；Active peptides can stimulate prawn pituitary gonadotrophin secretion thin Born of the same parents develop and enter secretory activity ahead of time, promote the differentiation of sexual gland, promote the development of sexual organ.
Embodiment 2：
The preparation method of prawn breeding period fortified feed, comprises the following steps：
1）Fermented bean dregs：150 parts of dregs of beans, 3 parts of molasses are weighed, it is 50% to add distillation water management water content, and regulation pH is 7.2, temperature Spend for 28 DEG C, be inoculated with bacillus licheniformis 2.5%, saccharomycete 2.0%, lactic acid bacteria 2.2%, once, fermentation 96 is small for every 36 hours stirrings When, low temperature dewatering produces fermented bean dregs；Microorganism can change microbiologic population's composition of gut of shrimp after entering in prawn body, Improve the prawn speed of growth, enhancing resistance against diseases, improve immunocompetence；
2）The preparation of tea polyphenol liposomes：Weigh 0.10mol/L, pH be 7.5 sodium dihydrogen phosphate mixed with disodium hydrogen phosphate it is molten 450 parts of liquid, 8.5 parts of Tea Polyphenols is slowly added to, 58 DEG C are heated under strong stirring and keeps constant temperature；Weigh egg yolk lecithin 280 Part, 35 parts of cholesterol, VE15 parts, are added in 300 parts of absolute ethyl alcohols, are heated to 58 DEG C of stirring and dissolvings, will be mixed after dissolving completely Close solution to add in Tea Polyphenols solution, keeping temperature strong stirring 60 minutes, then in 50 DEG C of temperature, 0.15MPa vacuum backspins Turn evaporation and remove residual ethanol in 35 minutes, be then ultrasonically treated, ultrasonic power 280W, frequency be 30 KHz, with ultrasonic 5 seconds, The interval frequency ultrasound of 4 seconds is handled 100 seconds, and tea polyphenol liposomes are placed in 6 DEG C of preservations by ultrasound after terminating；Tea prepared by the method Polyphenol liposome encapsulation is high, is easy to the absorption of prawn, and preparation method is simple, is avoided in preparation process containing ether etc. The physics and chemistry processing of potentially harmful substance, is especially suitable for industrialization large-scale production；
3）Compounding：Weigh 45 parts of fermented bean dregs, 12 parts of congealed fat soya-bean oil, 3 parts of blood meal, 2 parts of meat meal tankage, 5 parts of dusty yeast, salt 5.0 Part, 1.5 parts of calcium dihydrogen phosphate, 6.0 parts of krill meal, 6.0 parts of cuttlefish powder, 0.04 part of active peptides, 0.5 part of Choline Chloride, ox sulphur 1.5 parts of acid, 2.5 parts of vitamin premix, 1.2 parts of mineral substance premix, 0.06 part of calcium propionate, 0.06 part of ethoxyquin beautiful jade, sea 0.5 part of 0.7 part of mosanom, 1.0 parts of cholesterol, 0.5 part of alanine, 0.5 part of 5- inosinyl phosphate inosines, 0.5 part of dried orange peel, brewex's grains mixing The sub-sieve of 100 mesh is crossed after crushing, particle is sifted out and pulverizes and sieves again, feed meal is mixed 15 minutes with mixer again, and injection is made Grain machine wet granulation, particle diameter 4mm, produces the fortified feed of prawn breeding period；In feed rich in protein, amino acid, fat, The nutriments such as vitamin, mineral element, are aided with phagostimulant, can meet the nutritional requirement needed for close shrimp growth and development, promote Enter prawn growth and gonad development, be ready for its breeding, be it is a kind of be efficiently directed to, the safe and non-toxic secondary prawn remained breeds The nutrient enrichment fodder of phase.
Embodiment 3：
The preparation method of prawn breeding period fortified feed, including：Fermented bean dregs, the preparation of tea polyphenol liposomes, compounding, specific bag Include following steps：
Fermented bean dregs：120 parts of dregs of beans, 3 parts of molasses are weighed, it is 50% to add distillation water management water content, and regulation pH is 7.0, temperature For 25 DEG C, bacillus licheniformis 2.5% is inoculated with, saccharomycete 2.0%, lactic acid bacteria 2.0%, once, fermentation 96 is small for every 24 hours stirrings When, low temperature dewatering produces fermented bean dregs；Through everfermentation, the ANFs in dregs of beans is decomposed removal, rich in protein, organic Matter, middle trace element, it is suitable as the base-material of prawn feed；Microorganism can change the micro- of gut of shrimp after entering in prawn body Biocoene forms, and improves the prawn speed of growth, enhancing resistance against diseases, improves immunocompetence；
The preparation of tea polyphenol liposomes：Weigh the sodium dihydrogen phosphate and disodium hydrogen phosphate mixed solution that 0.10mol/L, pH are 7.4 420 parts, 8.4 parts of Tea Polyphenols is slowly added to, 56 DEG C are heated under strong stirring and keeps constant temperature；Weigh 250 parts of egg yolk lecithin, 32 parts of cholesterol, VE12 parts, it is added in 280 parts of absolute ethyl alcohols, is heated to 55 DEG C of stirring and dissolvings, will be mixed after dissolving completely molten Liquid is added in Tea Polyphenols solution, keeping temperature strong stirring 50 minutes, is then rotated and is steamed under 50 DEG C of temperature, 0.12MPa vacuum Hair removes residual ethanol in 30 minutes, is then ultrasonically treated, ultrasonic power 260W, and frequency is 28 KHz, with ultrasonic 4 seconds, interval 4 Second frequency ultrasound handle 100 seconds, tea polyphenol liposomes are placed in 5 DEG C of preservations by ultrasound after terminating；Tea Polyphenols prepared by the method Liposome encapsulation is high, is easy to the absorption of prawn, and preparation method is simple, is avoided in preparation process potential containing ether etc. The physics and chemistry processing of harmful substance, is especially suitable for industrialization large-scale production；
Compounding：Weigh 460 parts of fermented bean dregs, 12 parts of congealed fat soya-bean oil, 3 parts of blood meal, 2 parts of meat meal tankage, 3 parts of dusty yeast, salt 3.0 Part, 1.0 parts of calcium dihydrogen phosphate, 6.0 parts of krill meal, 5.0 parts of cuttlefish powder, 0.04 part of active peptides, 0.5 part of Choline Chloride, ox sulphur 1.5 parts of acid, 2.5 parts of vitamin premix, 1.2 parts of mineral substance premix, 0.06 part of calcium propionate, 0.06 part of ethoxyquin beautiful jade, sea 100 purposes are crossed after 0.4 part of 0.7 part of mosanom, 1.0 parts of cholesterol, 0.2 part of dried orange peel, 0.4 part of brewex's grains, dried silkworm chrysalis meal co-grinding Sub-sieve, sift out particle and pulverize and sieve again, feed meal is mixed 15 minutes with mixer again, injects comminutor wet granulation, grain Footpath is 3mm, produces the fortified feed of prawn breeding period；The amino acid sequence of active peptides is： SCASRCKSRCRARRCRARRCGYYYFGKRCICKCFRC；Active peptides can stimulate prawn pituitary gonadotrophin secretion thin Born of the same parents develop and enter secretory activity ahead of time, promote the differentiation of sexual gland, promote the development of sexual organ；Rich in protein, ammonia in feed The nutriment such as base acid, fat, vitamin, mineral element, is aided with phagostimulant, can meet the battalion needed for close shrimp growth and development Foster requirement, the growth of promotion prawn and gonad development, are ready for its breeding, are that one kind is efficiently directed to, safe and non-toxic pair remains Prawn breeding period nutrient enrichment fodder.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should be included in the scope of the protection.
Sequence table
<110>Animal feed technological development Co., Ltd is full of by Pujiang County association
<120>The preparation method of prawn breeding period fortified feed
<130> 1
<160> 1
<170> SIPOSequenceListing 1.0
<210> 2
<211> 36
<212> PRT
<213>Artificial synthesized (Mytilus coruscus)
<400> 2
Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Arg
1 5 10 15
Ala Arg Arg Cys Gly Tyr Tyr Tyr Phe Gly Lys Arg Cys Ile Cys Lys
20 25 30
Cys Phe Arg Cys
35

Claims (8)

1. the preparation method of prawn breeding period fortified feed, including：Fermented bean dregs, the preparation of tea polyphenol liposomes, compounding, it is special Sign is：Described compounding step is：It is pre- to weigh fermented bean dregs, congealed fat soya-bean oil, active peptides, vitamin premix, mineral matter The sub-sieve of 80-100 mesh is crossed after batch mixing, composite feeding promoting agent co-grinding, particle is sifted out and pulverizes and sieves again, feed meal is again with mixed Conjunction machine mixing 10-15 minutes, comminutor wet granulation is injected, particle diameter 2-4mm, produces the fortified feed of prawn breeding period.
2. the preparation method of prawn breeding period fortified feed according to claim 1, it is characterised in that：Described fermentation beans Dregs of rice step is：Dregs of beans 100-150 parts, molasses 2-3 parts are weighed, it is 40-50% to add distillation water management water content, and regulation pH is 6.8-7.2, temperature are 23-28 DEG C, are inoculated with bacillus licheniformis 2.0-2.5%, saccharomycete 1.8-2.0%, lactic acid bacteria 2.0-2.2%, Per 24-36 hours stirring once, ferment 72-96 hours, low temperature dewatering produces fermented bean dregs.
3. the preparation method of prawn breeding period fortified feed according to claim 1, it is characterised in that：Described Tea Polyphenols The preparation process of liposome is：The sodium dihydrogen phosphate that 0.08-0.10mol/L, pH are 7.3-7.5 is weighed to mix with disodium hydrogen phosphate Solution 400-450 parts, Tea Polyphenols 8.2-8.5 parts are slowly added to, 55-58 DEG C is heated under strong stirring and keeps constant temperature；Weigh Egg yolk lecithin 250-280 parts, cholesterol 32-35 parts, VE10-15 parts, are added in 200-300 part absolute ethyl alcohols, are heated to 55-58 DEG C of stirring and dissolving, mixed solution is added in Tea Polyphenols solution after dissolving completely, 50-60 points of keeping temperature strong stirring Clock, then rotary evaporation 30-35 minutes remove residual ethanol under 45-50 DEG C of temperature, 0.10-0.15MPa vacuum, then ultrasonic Processing, ultrasonic power 250-280W, frequency is 28-30 KHz, is handled with the frequency ultrasound of ultrasonic 4-5 seconds, interval 3-4 seconds Tea polyphenol liposomes are placed in 4-6 DEG C of preservation by 90-100 seconds, ultrasound after terminating.
4. the preparation method of prawn breeding period fortified feed according to claim 1, it is characterised in that：Described compounding step Suddenly it is：Weigh fermented bean dregs 45-60 parts, congealed fat soya-bean oil 10-12 parts, blood meal 2-3 parts, meat meal tankage 1-2 parts, dusty yeast 3-5 parts, food Salt 3.0-5.0 parts, calcium dihydrogen phosphate 1.0-1.5 parts, krill meal 5.0-6.0 parts, cuttlefish powder 5.0-6.0 parts, active peptides 0.03- 0.04 part, Choline Chloride 0.3-0.5 parts, taurine 1.0-1.5 parts, vitamin premix 2.0-2.5 parts, mineral substance premix 1.0-1.2 parts, calcium propionate 0.05-0.06 parts, ethoxyquin beautiful jade 0.05-0.06 parts, sodium alginate 0.6-0.7 parts, cholesterol The sub-sieve of 80-100 mesh is crossed after 0.8-1.0 parts, composite feeding promoting agent 1.0-2.0 part co-grindings, particle is sifted out and crushed again Sieve, feed meal inject comminutor wet granulation, particle diameter 2-4mm, produce prawn and breed again with mixer mixing 10-15 minutes The fortified feed of phase.
5. the preparation method of prawn breeding period fortified feed according to claim 4, it is characterised in that：Described compounding step The amino acid sequence of active peptides in rapid is：SCASRCKSRCRARRCRARRCGYYYFGKRCICKCFRC.
6. the preparation method of prawn breeding period fortified feed according to claim 5, it is characterised in that：Described compounding step Vitamin premix in rapid is VA or VB1Or VB2Or VB6Or VB12Or VC or VD or VE or VK or calcium pantothenate or nicotinic acid or folic acid Or biotin or at least five kinds of mixture of inositol or microcrystalline cellulose.
7. the preparation method of prawn breeding period fortified feed according to claim 5, it is characterised in that：Described compounding step Mineral substance premix in rapid is MgSO4·7H2O and CuSO4·5H2O and FeSO4·H2O and ZnSO4·H2O and MnSO4·H2O With CoCl2·H2O and Ca (IO3)2With Na2SeO3With the mixture of zeolite powder.
8. the preparation method of prawn breeding period fortified feed according to claim 5, it is characterised in that：Described compounding step Composite feeding promoting agent in rapid for glycine betaine or neopentanoic acid thetin or glycine or alanine or 5- inosinyl phosphate inosines or dried orange peel or At least three kinds of mixture in brewex's grains or dried silkworm chrysalis meal.
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