CN107646879A - A kind of biological agent for preventing and treating powdery mildew of strawberry - Google Patents
A kind of biological agent for preventing and treating powdery mildew of strawberry Download PDFInfo
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- CN107646879A CN107646879A CN201710877568.5A CN201710877568A CN107646879A CN 107646879 A CN107646879 A CN 107646879A CN 201710877568 A CN201710877568 A CN 201710877568A CN 107646879 A CN107646879 A CN 107646879A
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- biological agent
- strawberry
- powdery mildew
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of biological agent for preventing and treating powdery mildew of strawberry, belong to biological pesticide technical field.The biological agent includes active component metconazole, biomass and auxiliary agent.Proved through Toxicity Determination, active component metconazole and the biomass compounding of biological agent of the present invention have significant synergies；Field test proves that the preventive effect of biological agent of the present invention is substantially better than control group, and toxic side effect is small.
Description
Technical field
The present invention relates to technical field of pesticide, more particularly to a kind of biological agent for preventing and treating powdery mildew of strawberry.
Background technology
The applicant has found that illness occurs in the strawberry in ground when holiday time does farm work.Confirmed as by lookup data
Powdery mildew of strawberry, and learn strawberryIn vainPowder disease is the Major Diseases in strawberry production, and in recent years, the generation of powdery mildew of strawberry is gradual
Aggravate, particularly protecting field powdery mildew of strawberry, when occurring serious, sick leaf rate is more than 45%, and diseased fruit rate is more than 50%, seriously
It has impact on the yield, quality and economic benefit of strawberry.It is 20 DEG C that strawberry powdery mildew, which produces conidial preference temperature, 5 DEG C with
It is lower not fallen ill with more than 35 DEG C.Sporangium easily ruptures in water and discharges spore.Conidia germination and germ tube elongation
Environmental optima condition is：15~25 DEG C of temperature, relative humidity 75%-98%.Intense light irradiation substantially suppresses spore germination and mycelia
Growth.Decline with time lengthening conidium viability, but infect after 5 months and still have that part conidium is active, it is mitogenetic
Germination rate of the spore on young leaves is apparently higher than old leaf.Rainwater, which has, suppresses the pathogenetic effect of white powder, and spore is flown afternoon with fine day
Dissipate most, rainfall then strong inhibition spore dispersal.Leaf back is compared with susceptible disease, because leaf back stomata is more, and leaf back cuticula compared with
Thin, germ easily invades.Survive the winter or more the viability of summer germ and pathogenicity depend on conidial production.White powder is critically ill
Blade, petiole, flower, fruit and the carpopodium of evil strawberry.Strawberry Leaves early stage, very thin white hypha is grown on blade face
Layer, with aggravation, leaf margin is gradually scrolled up, and dark-coloured stain and the white powder thing to differ in size, later stage are produced on blade
In bronzing scab, blade edge atrophy is shrivelled, bud and spend it is susceptible after, petal is changed into red, and bud can not open.Fruit
After susceptible, young fruit can not normally expand, and dry up.Fruit face is covered with one layer of white powder thing, tarnishes and hardens.At present, the disease
Also based on chemical pesticide control, its drug effect Residual time length, ecological environment is had a strong impact on.Therefore, the applicant utilizes what is learned
Biological knowledge, with the help of relevant information, a kind of biological agent for preventing and treating powdery mildew of strawberry is developed, has carried out field examination
Test, achieve preferable effect.
The content of the invention
It is an object of the invention to provide a kind of biological agent for preventing and treating powdery mildew of strawberry.
The purpose of the present invention is achieved through the following technical solutions, and the biological agent includes active component metconazole, life
Material and auxiliary agent.
Described active ingredient metconazole 3-7 parts, biomass 13-19 parts.
The auxiliary agent includes phenethyl phenol polyethenoxy ether 1-3 parts, ethylene glycol 1-3 parts, 2,6- di-t-butyl -4- methylbenzenes
Phenol 0.8-1.2 parts, polyvinyl alcohol 0.8-1.4 parts, triethanolamine 0.5-0.9 parts.
The biomass is crushed by Japanese Stephania Root, 80% ethanol of 10 times of amounts of addition, dipping 3 times, 18 hours every time, mistake
Filter, merge maceration extract, be recovered under reduced pressure to without alcohol taste and be made.
The preparation of biological agent of the present invention：By the metconazole weighed, phenethyl phenol polyethenoxy ether, 2,6- di-t-butyls-
4- methylphenols and ethylene glycol are added in reactor, and being heated to 60 DEG C with 30 DEG C/min speed melts active compound, and are stirred equal
Even obtained oil phase.The polyvinyl alcohol weighed, biomass, triethanolamine and suitable quantity of water are well mixed, and are heated slowly to 70 DEG C,
Aqueous phase is made.Oil phase is slowly injected into aqueous phase, sheared using high-speed intermittent cutter, shear time is 50-70 points
Clock, and room temperature is cooled down to, biological agent of the present invention is made.
Embodiment
Embodiment one：The biological agent is by metconazole 3g, phenethyl phenol polyethenoxy ether 1g, ethylene glycol 1g, 2,6- bis-
Tert-butyl-4-methyl-Phenol 0.8g, polyvinyl alcohol 0.8g, biomass 13g, triethanolamine 0.5g are made.The biomass be by
Japanese Stephania Root crush, add 10 times amount 80% ethanol, impregnate 3 times, 18 hours every time, filtering, merge maceration extract, be recovered under reduced pressure to
It is made without alcohol taste.By the metconazole weighed, phenethyl phenol polyethenoxy ether, 2,6- di-tert-butyl-4-methy phenols and ethylene glycol
Add in reactor, being heated to 60 DEG C with 30 DEG C/min speed melts active compound, and the obtained oil phase that stirs.By what is weighed
Polyvinyl alcohol, biomass, triethanolamine and suitable quantity of water are well mixed, and are heated slowly to 70 DEG C, and aqueous phase is made.Oil phase is slow
Inject in aqueous phase, sheared using high-speed intermittent cutter, shear time is 60 minutes, and cools down to room temperature, is made
Biological agent of the present invention.
Embodiment two：The biological pesticide is by metconazole 5g, phenethyl phenol polyethenoxy ether 2g, ethylene glycol 2g, 2,6- bis-
Tert-butyl-4-methyl-Phenol 1g, polyvinyl alcohol 1.1g, biomass 16g, triethanolamine 0.7g are made, the same embodiment party of preparation method
Formula one.
Embodiment three：The biological pesticide is by metconazole 7g, phenethyl phenol polyethenoxy ether 3g, ethylene glycol 3g, 2,6- bis-
Tert-butyl-4-methyl-Phenol 1.2g, polyvinyl alcohol 1.4g, biomass 19g, triethanolamine 0.9g are made, and preparation method is the same as implementation
Mode one.
Heat storage stability is tested
10g biological agents of the present invention are injected in clean ampoule bottle with syringe, sealed rapidly with thermal-flame, will be close
The ampoule bottle being honored as a queen is placed in 50 DEG C of baking oven, is taken out after static heat storage 20d, detection outward appearance without layering, mobility, dispersiveness,
The indices such as bioactivity are unchanged before being store with heat.Biological agent heat storage of the present invention is qualified.Its result is equivalent to storage at normal temperature 2
Year qualified products.
Low-temperature stability is tested
10g biological agents of the present invention are injected in clean ampoule bottle with syringe and (avoid sample contacts bottleneck), with height
Warm fire flame (alcohol blast burner) sealing (avoiding solvent from volatilizing) rapidly, the ampoule bottle after sealing is placed under 0 DEG C of low temperature after cold storage 20d
Take out, detection observation outward appearance is unchanged before being store without the indices such as layering, mobility, dispersiveness, bioactivity with heat, is low temperature
Qualified stability.
Freeze-thaw stability is tested
10g samples to be tested are injected in clean ampoule bottle with syringe and (avoid sample contacts bottleneck), use thermal-flame
(alcohol blast burner) sealing (avoiding solvent from volatilizing) rapidly, after the ampoule bottle after sealing is placed in 15 under -18 DEG C of low temperature, by ampoule bottle
10h at a temperature of taking-up is placed in 20 DEG C, this process are considered as one cycle, are checked after circulating in triplicate, and sample separates out without solid is
It is qualified.
Dilution stability is tested in 150mL beakers, is added 100mL32 DEG C of ± 4 DEG C of standard hard waters, is fitted with pipette, extract
Biological agent of the present invention is measured, is being slowly added into the case of being stirred continuously in hard water, it is made into 100mL emulsion.After adding,
Continue the speed stirring 30s with 6r/s, emulsion is moved into cleaning immediately, dried in 100mL graduated cylinders, and graduated cylinder is placed in constant temperature
In water-bath, in the range of 29 DEG C ± 3 DEG C of temperature, stand 2h, take out, observation emulsion in graduated cylinder without oil slick (cream), heavy oil and
Precipitation, then it is qualified to judge stability of emulsion.
Field control effectiveness test
The use of by this bio-chemical control made of embodiment two is experimental group；Wettable powder is restrained using 50% Tobe
Agent preventing and treating for preventing and treating group；Metconazole active compound only is used, is according to what medicine A made of the preparation method in embodiment was prevented and treated
A groups；Main ingredient biomass only is used, is B groups according to medicine B preventing and treatings made of the preparation method in embodiment；Clear water is CK,
Each group dilutes 600 times of liquid.
Experimental field positioned at Linyi City Yinan County strawberry cultivating, powdery mildew of strawberry is sent out, before experiment during 7 days and experiment
Other agricultural chemicals are not applied.Experimental field soil property is medium, and rich water is medium, and management is consistent.Temperature is put down at 22-29 degrees Celsius during experiment
Equal 27 degrees Celsius of temperature；5 processing are set in this field test.Each processing is repeated 2 times, and totally 10 processing cells, select growing way
Basically identical field originates the 1st medication of phase in powdery mildew of strawberry with falling ill, and hereafter every 7d medications 1 time, continuous 2 times, uses
Knapsack sprayer, blade and stem are uniform by medicine.Second of medicine is applied after being spaced 7 days after first time dispenser, common dispenser is twice.Apply
Proceed by investigation within 7 days after medicine, take five point sampling, pinpoint adventive, determine the method for the indefinite leaf of the number of sheets, every is investigated 12
Blade (bottom 4 is opened, and middle part 4 is opened, and top 4 is opened), the classification investigation state of an illness, counts the incidence of disease and disease index, calculates prevention effect.
Severity Scaling standard：0 grade, disease-free spot on leaf；1 grade, lesion area accounts for whole leaf area below 1/4；2 grades, scab
Area accounts for the 1/4~1/2 of whole leaf area；3 grades, lesion area accounts for the 1/2~3/4 of whole leaf area；4 grades, lesion area accounts for
More than the 3/4 of whole leaf area.
Disease index (%)=∑ (number of blade of the sick level × sick level) × 100/ highest disease level × total leaf number.
Prevention effect (the %)=control disease index of (control disease index-processing disease index) × 100/.
Experimental result：Prevention effect of the different disposal of table 3 to mulberry tree powdery mildew
As seen from the above table, twice after dispenser, experimental group preventive effect is best.
Toxicity Determination
The assay method recommended with reference to FAO (FAO (Food and Agriculture Organization of the United Nation)).Indoor virulence of three kinds of medicaments to powdery mildew of strawberry
Measurement result such as following table.
| Medicament title | Time/hour | Virulence regression equation | EC50 | Co-toxicity coefficient |
| Biological agent | 48 | Y=2.101x+2.993 | 2.181mg/L | 129.1 |
| Medicine A | 48 | Y=1.314x+2.441 | 98.16mg/L | - |
| Medicine B | 48 | Y=1.743x+0.897 | 224.82mg/L | - |
Biomass and the connection of metconazole mixture in biological agent of the present invention are evaluated using the co-toxicity coefficient method of the abundant propositions of Sun Yun
Cooperation is used, and is antagonism if the co-toxicity coefficient (CTC) of mixture is less than 80；80~120 be summation action；More than 120, to increase
Effect acts on.Therefore, the synergy of main ingredient metconazole and biomass mixture is synergistic effect in biological agent of the present invention, this with
Upper experimental result is consistent.
When the Pesticide Residue of strawberry after we study dispenser, but have been surprisingly found that strawberry nutrition composition is varied from.
Its concrete condition is as shown in the table.
Experimental result：Influence of the different disposal to strawberry nutrition composition (per the content of 100g nutriments)
As seen from the above table, after using agricultural chemicals of the present invention, nutritional ingredient is better than other each groups, particularly non agricultural chemical residuum.
Claims (5)
1. a kind of biological agent for preventing and treating powdery mildew of strawberry, it is characterised in that the biological agent includes active component metconazole, life
Material and auxiliary agent.
A kind of 2. biological agent for preventing and treating powdery mildew of strawberry as claimed in claim 1, it is characterised in that the active ingredient leaf
Bacterium azoles 3-7 parts, biomass 13-19 parts.
3. a kind of biological agent for preventing and treating powdery mildew of strawberry as claimed in claim 1, it is characterised in that the auxiliary agent includes benzene
Ethyl phenol polyethenoxy ether 1-3 parts, ethylene glycol 1-3 parts, 2,6- di-tert-butyl-4-methy phenol 0.8-1.2 parts, polyvinyl alcohol
0.8-1.4 parts, triethanolamine 0.5-0.9 parts.
A kind of 4. biological agent for preventing and treating powdery mildew of strawberry as claimed in claim 1, it is characterised in that the biomass be by
Japanese Stephania Root crush, add 10 times amount 80% ethanol, impregnate 3 times, 18 hours every time, filtering, merge maceration extract, be recovered under reduced pressure to
It is made without alcohol taste.
5. a kind of preparation method for the biological agent for preventing and treating powdery mildew of strawberry, it is characterised in that by the metconazole weighed, phenethyl
Phenol polyethenoxy ether, 2,6- di-tert-butyl-4-methy phenols and ethylene glycol are added in reactor, are heated with 30 DEG C/min speed
Active compound is melted to 60 DEG C, and the obtained oil phase that stirs；By the polyvinyl alcohol weighed, biomass, triethanolamine and suitable quantity of water
It is well mixed, and 70 DEG C are heated slowly to, aqueous phase is made；Oil phase is slowly injected into aqueous phase, uses high-speed intermittent cutter
Sheared, shear time is 50-70 minutes, and cools down to room temperature, and biological agent of the present invention is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710877568.5A CN107646879A (en) | 2017-09-15 | 2017-09-15 | A kind of biological agent for preventing and treating powdery mildew of strawberry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710877568.5A CN107646879A (en) | 2017-09-15 | 2017-09-15 | A kind of biological agent for preventing and treating powdery mildew of strawberry |
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| Publication Number | Publication Date |
|---|---|
| CN107646879A true CN107646879A (en) | 2018-02-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710877568.5A Pending CN107646879A (en) | 2017-09-15 | 2017-09-15 | A kind of biological agent for preventing and treating powdery mildew of strawberry |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108849993A (en) * | 2018-08-07 | 2018-11-23 | 沂南县迎辉农业开发有限公司 | A kind of biological agent and preparation method for preventing and treating mulberry tree powdery mildew |
| CN112715582A (en) * | 2020-12-29 | 2021-04-30 | 南京启佑生物科技有限公司 | Preparation method and application of pesticide special for strawberries |
-
2017
- 2017-09-15 CN CN201710877568.5A patent/CN107646879A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108849993A (en) * | 2018-08-07 | 2018-11-23 | 沂南县迎辉农业开发有限公司 | A kind of biological agent and preparation method for preventing and treating mulberry tree powdery mildew |
| CN112715582A (en) * | 2020-12-29 | 2021-04-30 | 南京启佑生物科技有限公司 | Preparation method and application of pesticide special for strawberries |
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Application publication date: 20180202 |