CN102428965A - Specialized form spore powder of helminthosporium carposaprum echinochloa crusgalli - Google Patents
Specialized form spore powder of helminthosporium carposaprum echinochloa crusgalli Download PDFInfo
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- CN102428965A CN102428965A CN2011102834678A CN201110283467A CN102428965A CN 102428965 A CN102428965 A CN 102428965A CN 2011102834678 A CN2011102834678 A CN 2011102834678A CN 201110283467 A CN201110283467 A CN 201110283467A CN 102428965 A CN102428965 A CN 102428965A
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- spore
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Abstract
Specialized form spores powder of helminthosporium carposaprum echinochloa crusgalli belongs to the technical field of weeding fungi pesticide. The spore powder comprises the following raw material by weight in parts: weeding fungi specialized form spore powder of the helminthosporium carposaprum echinochloa crusgalli, stabilizer, vegetable oil and surfactant. Aiming at the specialized form industrialization needs of the microbial herbicide which is the helminthosporium carposaprum echinochloa crusgalli, the invention mixes the auxiliaries, such as surfactant, the stabilizer and antioxidant, to form the stable spore powder. The spore powder is sprayed through a normal sprayer after directly added to water according to rate while being used, so the use is convenient. The specialized form spores powder provided by the invention can prolong the storage time for over one year and have high preventing rate of the echinochloa crusgalli. The average preventing rate is over 65%, and the highest preventing rate is over 90%.
Description
Technical field
The invention belongs to weeding fungal farm chemicals technical field, the standing grain length that is specifically related to a kind of weeding spore barnyard grass grass specialized form (having another name called Goosegrass bipolaris) the spore pulvis of wriggling.
Background technology
The barnyard grass grass is the rice field malignant weed, if do not prevent and kill off having a strong impact on rice yield, mainly prevents and kill off through chemical herbicide at present.Though chemical herbicide can be prevented and kill off barnyard grass in paddy field fast; But long-term a large amount of uses also bring a lot of negative effects, increase like barnyard grass grass pesticide resistance biotype; Farmland, some areas and water environment are contaminated, to environment and ecological safety and agricultural sustainable development formation potential threat etc.Microbial herbicide is the biologic product that utilizes biological living or bioactivator exploitation; Have environmentally safe, noresidue and be difficult for making advantages such as crop develops immunity to drugs; Thereby be that the fine of chemical herbicide replenished and substitute, receive various countries scientist's generally attention.The standing grain length spore barnyard grass grass specialized form of wriggling is to separate from natural infected cockspur grass to obtain weeds biological and ecological methods to prevent plant disease, pests, and erosion potentiality bacterium, to crop safeties such as paddy rice, wheat, corn, cotton and rapes.The standing grain length spore barnyard grass grass specialized form spore of wriggling has stronger pathogenicity to the barnyard grass grass, and its suspending agent can reach 85.2% to the highest preventive effect of barnyard grass grass, average preventive effect about 50%.Spore barnyard grass grass specialized form spore suspending agent has higher preventive effect to weeds in paddy field such as barnyard grass grass though standing grain length is wriggled, and this formulation is difficult for long term storage, and spore suction back is prone to sprout and lost efficacy.Therefore, developing a kind of standing grain length that both can prolong and wriggle the spore barnyard grass grass specialized form spore storage time, can guarantee the formulation of its herbicidal effect again, is the wriggle important topic of spore barnyard grass grass specialized form large scale application of present standing grain length.
Summary of the invention
To the problem that prior art exists, the objective of the invention is to design provides a kind of anti-storage, easy to use, standing grain length technical scheme of spore barnyard grass grass specialized form spore pulvis of wriggling efficiently stablized.
The described standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that containing the raw material of following weight parts:
Wriggle 50～75 parts of spore barnyard grass grass specialized form conidial powder of weeding fungi standing grain length
5～20 parts of stabilizing agents
10～25 parts of vegetable oil
5～15 parts in surfactant.
The described standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that containing the raw material of following weight parts:
Wriggle 55～70 parts of spore barnyard grass grass specialized form conidial powder of weeding fungi standing grain length
8～15 parts of stabilizing agents
12～20 parts of vegetable oil
5～10 parts in surfactant.
The described standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described stabilizing agent is one or more mixtures in sodium alginate, sodium carboxymethylcellulose and the potassium alginate.
The described standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described vegetable oil is one or more mixtures in soybean oil, corn oil and the rapeseed oil.
The described standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described surfactant is a nonionic surface active agent.
The described standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that also containing the antioxidant that accounts for each raw material gross weight 0.1～1.0%.
The described standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that also containing the uv-protector that accounts for each raw material gross weight 0.1～1.0%.
The described standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described nonionic surface active agent is one or more mixtures in sapn and the tween.
The described standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described antioxidant is one or more mixtures in BHA, BHT and the vitamin E.
The described standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described uv-protector is one or more mixtures in Tea Polyphenols, fluorescein sodium and the ascorbic acid.
The selection principle of above auxiliary agent is and the compatible synergy of spore barnyard grass grass specialized form spore of wriggling of standing grain length.
The present invention is applicable to the wriggle processing and fabricating of spore barnyard grass grass specialized form conidial powder of the weeding fungi standing grain length that obtains through liquid-solid fermentation.The weeding fungi standing grain length spore barnyard grass grass specialized form conidial powder of wriggling is to be 201010209596.8 through application number, and the method for disclosure of the Invention file record that name is called the cultural method of helminthosporium sporangium spores obtains.
The standing grain length spore barnyard grass grass specialized form spore pulvis processing method flow process of wriggling:
1) utilize the high shear dispersion machine to mix surfactant, antioxidant and vegetable oil by said weight ratio, subsequent use;
2) adopt the spraying stirring technique, said vegetable oil mixt of step 1) and the weeding fungi standing grain length spore barnyard grass grass specialized form conidial powder of wriggling is mixed formation skim protectiveness oil film by said weight ratio;
3) in said ratio with step 2) wriggle spore barnyard grass grass specialized form conidial powder and stabilizing agent, uv-protector of gained weeding fungi standing grain length mix in agitator and can obtain the standing grain length careless specialized form spore of the spore barnyard grass pulvis of wriggling.
The present invention is directed to the microbial herbicide standing grain length spore barnyard grass grass specialized form industrialization needs of wriggling; Compatibility principle according to mycobiology characteristic and the compatible synergy of microbial pesticide; According to optimization of C auxiliary component mixed preparing such as surfactant, stabilizing agent, antioxidant are formed stable spore pulvis; Adopt conventional sprayer to spray behind the direct in proportion thin up during use and get final product, easy to use.And the present invention can prolong the storage time, generally can store more than 1 year, and is high to the control rate of barnyard grass grass, and average control rate can reach more than 65%, and the highest control rate reaches more than 90%.
Embodiment
Further specify the present invention below in conjunction with specific embodiment.
Embodiment forms
Embodiment 1～5, and according to the form below is got different component, earlier surfactant, antioxidant, vegetable oil mixed, and adopts the spraying stirring technique again, and above-mentioned vegetable oil and the fungal spore powder that is mixed with surfactant and antioxidant mixed; At last fungal spore powder and other auxiliary agents are mixed in agitator and get final product.
The table 1 standing grain length spore barnyard grass grass specialized form conidial powder agent prescription (weight: gram or milliliter) of wriggling
Among the embodiment 1～5, the mixture that vegetable oil can adopt one or more arbitrary proportions in soybean oil, corn oil and the rapeseed oil to mix; The mixture that stabilizing agent can adopt one or more arbitrary proportions in sodium alginate, sodium carboxymethylcellulose and the potassium alginate to mix; Surfactant can adopt non-ionic surface active agent, like the mixture of one or more arbitrary proportions mixing in sapn and the tween, or other nonionic; The mixture that antioxidant can adopt one or more arbitrary proportions in BHA, BHT and the vitamin E to mix; The mixture that uv-protector can adopt one or more arbitrary proportions in Tea Polyphenols, fluorescein sodium and the ascorbic acid to mix.
Embodiment is to the control efficiency of weeds and to the safety evaluatio of paddy rice
(1) indoor biometrics result
Select for use paddy rice (
Oryza sativaL.) and barnyard grass grass (
Echinochloa crus-galli(L.) Beauv.) be the test target; The test water rice varieties is Japan fine (japonica rice), 9311 (long-grained nonglutinous rices) and osmanthus fragrant silk glutinous P106 (glutinous rice).
The greenhouse pot culture method is adopted in test, and it is the special-purpose soil of test of not medication plot collection that soil is used in test, gets bore 7.5cm, dark 6cm flowerpot, and soil is filled to 3/4 height.After adding water and treating that soil is fully moistening, each kind rice paddy seed program request that vernalization is showed money or valuables one carries unintentionally is to each flowerpot, and the barnyard grass grass directly broadcasts sowing.About every basin 20 strains, cover the thick mixed husky fine earth of 0.5～1.0cm, flowerpot bottom adds water makes the soil incubation growth in the saturated rearmounted greenhouse that absorbs water, and regular moisturizing is to keep 100% soil moisture, and temperature is between 20～35 ℃ in the greenhouse, and air humidity is more than 50%.Treat that paddy rice grew to for 3～3.5 leaf phases, the cauline leaf spraying is handled barnyard grass grass 2.5 leaves carry out bud during the phase after.
Adopt RD (90 g a.i./hm
2) under paddy rice and barnyard grass grass carried out bud after the cauline leaf spraying handle.3 repetitions are established in every processing, and other establishes blank.Handle in the rearmounted greenhouse and grow, paddy rice keeps certain water layer to cultivate, and weeds are handled and irritated 3～5cm water layer in back 24 hours.
Regularly observe the plant reaction symptom after the chemicals treatment, do not find that test medicine has visible symptom of chemical damage such as inhibition, chlorisis or deformity to the growth of paddy rice.30d " Invest, Then Investigate " paddy rice fresh weight, and to the activity of weeding of barnyard grass grass, result of the test (seeing table 2) shows that standing grain length wriggles spore barnyard grass grass specialized form spore pulvis to rice safety, can reach more than 80% the fresh weight inhibiting rate of barnyard grass grass.
Table 2 standing grain length is wriggled spore barnyard grass grass specialized form spore pulvis to safety of paddy rice and the preventive effect (indoor biometrics result) careless to barnyard grass
(2) field plot trial result
The confession rice varieties that tries the water is a japonica rice show water 09, and at 28 days length of time rice seedlings grow (d) during transplanting, seeding row spacing 15cmX15cm, single subdistrict area are 20m
2, each handles repetition 3 times.Transplant back 7～10d and handle with the standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling, consumption is 45～90 g a.i./hm
2, not dispenser blank and chemical herbicide contrast (60% butachlor EC, 110 mL/667m are established in test
2).7d, 14d and 28d investigation rice safety property and barnyard grass grass preventive effect after medication.
Result of the test is seen table 3.Observe the paddy growth situation behind the dispenser 7d, find not have obvious paddy rice damage symptom.28d " Invest, Then Investigate " paddy rice plant height and rice tillering number, find the embodiment various dose handle with not dispenser contrast between do not have significant difference, see that totally standing grain length wriggles the careless specialized form spore of spore barnyard grass pulvis to rice safety.Under 4 different tests dosage were handled, field barnyard grass grass blade number and plant fresh weight preventive effect all reached more than 70%, and the highest preventive effect surpasses 90%.
Table 3 standing grain length is wriggled spore barnyard grass grass specialized form spore pulvis to safety of paddy rice and the preventive effect (field test results) careless to barnyard grass
Annotate: "-" expression does not obviously damage symptom; Fate after " DAT " expression dispenser; Same letter representes that difference is not remarkable under 5% level in the same row of form.
Other embodiment test
Among the embodiment 6～10, the mixture that vegetable oil can adopt one or more arbitrary proportions in soybean oil, corn oil and the rapeseed oil to mix; The mixture that stabilizing agent can adopt one or more arbitrary proportions in sodium alginate, sodium carboxymethylcellulose and the potassium alginate to mix; Surfactant can adopt non-ionic surface active agent, like the mixture of one or more arbitrary proportions mixing in sapn and the tween, or other nonionic; The mixture that antioxidant can adopt one or more arbitrary proportions in BHA, BHT and the vitamin E to mix; The mixture that uv-protector can adopt one or more arbitrary proportions in Tea Polyphenols, fluorescein sodium and the ascorbic acid to mix.
Embodiment 6～10 through identical with embodiment 1～5 to weeds control efficiency and to the safety evaluatio test of paddy rice, average preventive effect reaches more than 65%, and good to rice safety property.
Claims (10)
1. the standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that containing the raw material of following weight parts:
Wriggle 50～75 parts of spore barnyard grass grass specialized form conidial powder of weeding fungi standing grain length
5～20 parts of stabilizing agents
10～25 parts of vegetable oil
5～15 parts in surfactant.
2. the standing grain length as claimed in claim 1 spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that containing the raw material of following weight parts:
Wriggle 55～70 parts of spore barnyard grass grass specialized form conidial powder of weeding fungi standing grain length
8～15 parts of stabilizing agents
12～20 parts of vegetable oil
5～10 parts in surfactant.
3. according to claim 1 or claim 2 the standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described stabilizing agent is one or more mixtures in sodium alginate, sodium carboxymethylcellulose and the potassium alginate.
4. according to claim 1 or claim 2 the standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described vegetable oil is one or more mixtures in soybean oil, corn oil and the rapeseed oil.
5. according to claim 1 or claim 2 the standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described surfactant is a nonionic surface active agent.
6. according to claim 1 or claim 2 the standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that also containing the antioxidant that accounts for each raw material gross weight 0.1～1.0%.
7. according to claim 1 or claim 2 the standing grain length spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that also containing the uv-protector that accounts for each raw material gross weight 0.1～1.0%.
8. the standing grain length as claimed in claim 5 spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described nonionic surface active agent is one or more mixtures in sapn and the tween.
9. the standing grain length as claimed in claim 6 spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described antioxidant is one or more mixtures in BHA, BHT and the vitamin E.
10. the standing grain length as claimed in claim 7 spore barnyard grass grass specialized form spore pulvis of wriggling is characterized in that described uv-protector is one or more mixtures in Tea Polyphenols, fluorescein sodium and the ascorbic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102834678A CN102428965A (en) | 2011-09-22 | 2011-09-22 | Specialized form spore powder of helminthosporium carposaprum echinochloa crusgalli |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102834678A CN102428965A (en) | 2011-09-22 | 2011-09-22 | Specialized form spore powder of helminthosporium carposaprum echinochloa crusgalli |
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| CN102428965A true CN102428965A (en) | 2012-05-02 |
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| CN2011102834678A Pending CN102428965A (en) | 2011-09-22 | 2011-09-22 | Specialized form spore powder of helminthosporium carposaprum echinochloa crusgalli |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039444A (en) * | 2012-12-31 | 2013-04-17 | 深圳诺普信农化股份有限公司 | Photolysis-resistant pesticide and application thereof |
| CN108684715A (en) * | 2018-07-05 | 2018-10-23 | 安徽袁粮水稻产业有限公司 | A kind of compounding improvement medicament and preparation method thereof for preventing barnyard grass |
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| CN1545885A (en) * | 2003-12-05 | 2004-11-17 | 重庆大学 | Fungi oil spore admixture |
| CN101658186A (en) * | 2009-09-16 | 2010-03-03 | 甘肃省科学院生物研究所 | Verticillium lecanii quick-release dispersible tablet and preparation method thereof |
| CN101878785A (en) * | 2010-06-26 | 2010-11-10 | 中国农业科学院兰州兽医研究所 | Tick-killing biological control agent and preparation method thereof |
| CN101886058A (en) * | 2010-06-24 | 2010-11-17 | 中国水稻研究所 | Culture method of helminthosporium sporangium spores |
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2011
- 2011-09-22 CN CN2011102834678A patent/CN102428965A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1438312A (en) * | 2002-02-10 | 2003-08-27 | 中国水稻研究所 | Method for producing Bicao anti-bateria spore using Bicao substrate |
| CN1545885A (en) * | 2003-12-05 | 2004-11-17 | 重庆大学 | Fungi oil spore admixture |
| CN101658186A (en) * | 2009-09-16 | 2010-03-03 | 甘肃省科学院生物研究所 | Verticillium lecanii quick-release dispersible tablet and preparation method thereof |
| CN101886058A (en) * | 2010-06-24 | 2010-11-17 | 中国水稻研究所 | Culture method of helminthosporium sporangium spores |
| CN101878785A (en) * | 2010-06-26 | 2010-11-10 | 中国农业科学院兰州兽医研究所 | Tick-killing biological control agent and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039444A (en) * | 2012-12-31 | 2013-04-17 | 深圳诺普信农化股份有限公司 | Photolysis-resistant pesticide and application thereof |
| CN108684715A (en) * | 2018-07-05 | 2018-10-23 | 安徽袁粮水稻产业有限公司 | A kind of compounding improvement medicament and preparation method thereof for preventing barnyard grass |
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Application publication date: 20120502 |