CN107646529A - A kind of carrier for silk ball bacteria cultivation - Google Patents
A kind of carrier for silk ball bacteria cultivation Download PDFInfo
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- CN107646529A CN107646529A CN201710725359.9A CN201710725359A CN107646529A CN 107646529 A CN107646529 A CN 107646529A CN 201710725359 A CN201710725359 A CN 201710725359A CN 107646529 A CN107646529 A CN 107646529A
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- China
- Prior art keywords
- carrier
- sparassis crispa
- oxamides
- endoxan
- ester
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Abstract
The invention discloses a kind of carrier for silk ball bacteria cultivation, including following composition：Acrylic monomers, oxamides, to ethoxy benzamide, 4 hydroperoxyl endoxan, 1,1 cyclohexanediacetic acid monoamides, potassium peroxydisulfate, tricresyl phosphate (butoxyethyl group) ester.Carrier prepared by the present invention, formula material is simple and easy to get, can more preferably preserve nutriment compared with agar so that Sparassis crispa yield is higher.
Description
Technical field
The present invention relates to a kind of mushroom cultivation carrier, more particularly to a kind of carrier for silk ball bacteria cultivation.
Background technology
Sparassis crispa, also known as silk ball mushroom, Classification system are Sparassis crispa, are non-pleat pore fungi mesh, silk ball Cordycepps, embroider
Coccus, its fructification meat is pure white fresh and tender, tasty, because wild resource is rare, belongs to one of rare wild edible fungus,
The ground such as China Hebei, Jilin, Heilungkiang, Guangdong are distributed in, are also distributed in Japan, Europe, Oceania, North America.Sparassis crispa
Activation immunocompetence with superelevation, containing substantial amounts of beta glucan, polyphenoils, ask dispelling the skin such as melanin deposition
Topic has good effects.In addition experiment proves that Sparassis crispa has good inhibition to tumour, particularly to breast cancer, stomach cancer
It is evident in efficacy, and also have good clinical effectiveness to hypertension, diabetes.
In the prior art, it is most of that the culture to Sparassis crispa, the main carriers of soilless culture are realized by soilless culture
It is agar.But agar can not play the ability of locking nutriment very well as carrier so that Sparassis crispa growing way is bad.Separately
The outer report for also having some at present on Sparassis crispa liquid fermentation technology, but still there is limitations applied to cultivation, generally
Problem be present：1st, hypha biomass is low；2nd, culture medium raw material source inconvenience；3rd, complex operation, it is unfavorable for large-scale production.Cause
This, it is significant to develop a kind of raw material sources cultural method that is extensive, easy to operate, being adapted to large-scale production.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of silk ball bacteria cultivation for being used to substitute agar
Carrier.
To achieve the above object, the present invention provides a kind of carrier for silk ball bacteria cultivation, including following composition：Acrylic acid
Monomer, oxamides, to ethoxy benzamide, 4- hydroperoxyls endoxan, 1,1- cyclohexanediacetic acids monoamides, persulfuric acid
Potassium, tricresyl phosphate (butoxyethyl group) ester.
Preferably, the present invention provides a kind of carrier for silk ball bacteria cultivation, including following parts by weight composition：
Acrylic monomers：200；
Oxamides：20；
To ethoxy benzamide：10；
4- hydroperoxyl endoxan：10；
1,1- cyclohexanediacetic acid monoamides：2；
Potassium peroxydisulfate：1；
Tricresyl phosphate (butoxyethyl group) ester：10.
The present invention also provides the preparation method of the above-mentioned carrier for silk ball bacteria cultivation：
(1) acrylic monomers is weighed, pH value is adjusted by sodium hydroxide, is configured to the sodium acrylate solution that pH is 6-7；
(2) oxamides, formyl phenetidin, 4- hydroperoxyl endoxan are added to be well mixed, is warming up to 30 DEG C, instead
Answer 30 minutes；
(3) 1,1- cyclohexanediacetic acids monoamides, potassium peroxydisulfate are added, 60 DEG C of reaction 1-2 hours is continuously heating to, obtains
Polymer；
(4) resulting polymers are mixed with tricresyl phosphate (butoxyethyl group) ester, reacts 20 minutes, carried at 40 DEG C
Body.
Above each component is weighed according to formulation weight part.
Embodiment
Embodiment 1
A kind of carrier for silk ball bacteria cultivation, including following parts by weight composition：
Acrylic monomers：200；
Oxamides：20；
To ethoxy benzamide：10；
4- hydroperoxyl endoxan：10；
1,1- cyclohexanediacetic acid monoamides：2；
Potassium peroxydisulfate：1；
Tricresyl phosphate (butoxyethyl group) ester：10.
Prepare by the following method：
(1) acrylic monomers is weighed, pH value is adjusted by sodium hydroxide, is configured to the sodium acrylate solution that pH is 6-7；
(2) oxamides, formyl phenetidin, 4- hydroperoxyl endoxan are added to be well mixed, is warming up to 30 DEG C, instead
Answer 30 minutes；
(3) 1,1- cyclohexanediacetic acids monoamides, potassium peroxydisulfate are added, 60 DEG C of reaction 1-2 hours is continuously heating to, obtains
Polymer；
(4) resulting polymers are mixed with tricresyl phosphate (butoxyethyl group) ester, reacts 20 minutes, carried at 40 DEG C
Body.
Above each component is weighed according to formulation weight part.
Comparative example 1
From agar as carrier.
In embodiment 1 and comparative example 1, the amount for choosing carrier is 200g, adds starch 25g, maltose 25g, albumen
Peptone 6g, potassium phosphate 0.2g, magnesium sulfate 0.2g obtain matrix as nutriment.Detection for example below 2.
Embodiment 2
By silk ball strain (preserving number：ACCC51488, purchased from Chinese agriculture microbial resources storehouse) it is inoculated in potato
Activate, 25 DEG C of cultivation temperature, cultivate 14 days on solid medium.It is seeded to respectively by embodiment 1 and contrast by 10% inoculum concentration
In matrix made from the carrier of example 1, then bottling carries out shaken cultivation 20 days under 25 DEG C, rotating speed 150rpm natural lightings,
6000rpm removes supernatant after centrifuging 10 minutes, obtains Sparassis crispa mycelium, measures its yield.
| Yield | |
| Embodiment 1 | 8.2g/L |
| Comparative example 1 | 0.6g/L |
As can be seen from the above table, carrier of the invention compared with agar nutriment can be played more preferable preservation effect so as to
Sparassis crispa is preferably grown, obtains higher yields.In addition, from composition, raw material of the invention be also it is simple and easy to get,
And performance is relatively stable.
The general principle of the present invention, principal character and advantages of the present invention, the technology of the industry has been shown and described above
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.
Claims (5)
1. a kind of carrier for silk ball bacteria cultivation, including following composition：Acrylic monomers, oxamides, to ethoxybenzene formyl
Amine, 4- hydroperoxyls endoxan, 1,1- cyclohexanediacetic acids monoamides, potassium peroxydisulfate, tricresyl phosphate (butoxyethyl group) ester.
2. a kind of carrier for silk ball bacteria cultivation, including following parts by weight composition：
Acrylic monomers：200；
Oxamides：20；
To ethoxy benzamide：10；
4- hydroperoxyl endoxan：10；
1,1- cyclohexanediacetic acid monoamides：2；
Potassium peroxydisulfate：1；
Tricresyl phosphate (butoxyethyl group) ester：10.
3. the preparation method according to claim 1 or 2 for being used to cultivate the carrier of Sparassis crispa, including：
(1) acrylic monomers is weighed, pH value is adjusted by sodium hydroxide, is configured to the sodium acrylate solution that pH is 6-7；
(2) oxamides, formyl phenetidin, 4- hydroperoxyl endoxan are added to be well mixed, is warming up to 30 DEG C, reaction 30
Minute；
(3) 1,1- cyclohexanediacetic acids monoamides, potassium peroxydisulfate are added, 60 DEG C of reaction 1-2 hours is continuously heating to, is polymerize
Thing；
(4) resulting polymers are mixed with tricresyl phosphate (butoxyethyl group) ester, is reacted 20 minutes at 40 DEG C, obtain carrier.
4. the carrier of claim 1 or 2 is used for the application for preparing Sparassis crispa matrix, it is characterised in that chooses carrier 200g, adds
Starch 25g, maltose 25g, peptone 6g, potassium phosphate 0.2g, magnesium sulfate 0.2g obtain matrix as nutriment.
5. application according to claim 4, it is characterised in that：Sparassis crispa is seeded in the matrix and cultivated, obtains Sparassis crispa
Mycelial yield is 8.2g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710725359.9A CN107646529A (en) | 2017-08-22 | 2017-08-22 | A kind of carrier for silk ball bacteria cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710725359.9A CN107646529A (en) | 2017-08-22 | 2017-08-22 | A kind of carrier for silk ball bacteria cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107646529A true CN107646529A (en) | 2018-02-02 |
Family
ID=61128565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710725359.9A Pending CN107646529A (en) | 2017-08-22 | 2017-08-22 | A kind of carrier for silk ball bacteria cultivation |
Country Status (1)
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101955392A (en) * | 2010-09-19 | 2011-01-26 | 福建省农业科学院食用菌研究所 | Formula of culture medium for industrial production of sparasis crispa and production process |
| CN106305143A (en) * | 2016-08-29 | 2017-01-11 | 张伟峰 | Carrier used for sparassis crispa cultivation |
| CN106365800A (en) * | 2016-08-29 | 2017-02-01 | 福建容益菌业科技研发有限公司 | Matrix for cultivating Sparassis crispa |
-
2017
- 2017-08-22 CN CN201710725359.9A patent/CN107646529A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101955392A (en) * | 2010-09-19 | 2011-01-26 | 福建省农业科学院食用菌研究所 | Formula of culture medium for industrial production of sparasis crispa and production process |
| CN106305143A (en) * | 2016-08-29 | 2017-01-11 | 张伟峰 | Carrier used for sparassis crispa cultivation |
| CN106365800A (en) * | 2016-08-29 | 2017-02-01 | 福建容益菌业科技研发有限公司 | Matrix for cultivating Sparassis crispa |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180202 |
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| RJ01 | Rejection of invention patent application after publication |