CN107636004A - 聚合酶变体及其用途 - Google Patents

聚合酶变体及其用途 Download PDF

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Publication number
CN107636004A
CN107636004A CN201680027667.8A CN201680027667A CN107636004A CN 107636004 A CN107636004 A CN 107636004A CN 201680027667 A CN201680027667 A CN 201680027667A CN 107636004 A CN107636004 A CN 107636004A
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CN
China
Prior art keywords
polymerase
modification
dna polymerase
archaeal dna
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201680027667.8A
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English (en)
Chinese (zh)
Inventor
A.艾尔
C.阿诺德
C.施瓦布
E.泰
I.莱德曼
C.麦克高
T.舒尔茨
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/012,317 external-priority patent/US10308918B2/en
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN107636004A publication Critical patent/CN107636004A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201680027667.8A 2015-05-14 2016-05-13 聚合酶变体及其用途 Pending CN107636004A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201562161571P 2015-05-14 2015-05-14
US62/161571 2015-05-14
US201562202895P 2015-08-09 2015-08-09
US62/202895 2015-08-09
US15/012317 2016-02-01
US15/012,317 US10308918B2 (en) 2015-02-02 2016-02-01 Polymerase variants
US15/151364 2016-05-10
US15/151,364 US10526588B2 (en) 2015-05-14 2016-05-10 Polymerase variants and uses thereof
PCT/US2016/032258 WO2016183403A2 (en) 2015-05-14 2016-05-13 Polymerase variants and uses thereof

Publications (1)

Publication Number Publication Date
CN107636004A true CN107636004A (zh) 2018-01-26

Family

ID=57248698

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680027667.8A Pending CN107636004A (zh) 2015-05-14 2016-05-13 聚合酶变体及其用途

Country Status (6)

Country Link
US (3) US10526588B2 (https=)
EP (1) EP3294749A4 (https=)
JP (3) JP2018515103A (https=)
CN (1) CN107636004A (https=)
CA (2) CA3028797C (https=)
WO (1) WO2016183403A2 (https=)

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US10526588B2 (en) * 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
EP3377648B1 (en) 2015-11-16 2021-01-06 Stratos Genomics, Inc. Dp04 polymerase variants
US10590480B2 (en) 2016-02-29 2020-03-17 Roche Sequencing Solutions, Inc. Polymerase variants
US20170268052A1 (en) 2016-02-29 2017-09-21 Genia Technologies, Inc. Polymerase-template complexes
CN108699539B (zh) * 2016-02-29 2022-11-18 吉尼亚科技公司 外切核酸酶缺陷的聚合酶
US10655174B2 (en) 2016-05-27 2020-05-19 Roche Sequencing Solutions, Inc. Tagged multi-nucleotides useful for nucleic acid sequencing
US10669580B2 (en) 2016-08-26 2020-06-02 Roche Sequencing Solutions, Inc. Tagged nucleotides useful for nanopore detection
JP7157048B2 (ja) 2016-09-22 2022-10-19 エフ．ホフマン－ラ ロシュ アーゲー Ｐｏｌ６ポリメラーゼバリアント
US11708566B2 (en) 2017-05-04 2023-07-25 Stratos Genomics, Inc. DP04 polymerase variants
CA3084812A1 (en) * 2017-12-11 2019-06-20 Stratos Genomics, Inc. Dpo4 polymerase variants with improved accuracy
WO2019166457A1 (en) 2018-02-28 2019-09-06 F. Hoffmann-La Roche Ag Tagged nucleoside compounds useful for nanopore detection
WO2019166458A1 (en) 2018-02-28 2019-09-06 F. Hoffmann-La Roche Ag Alpha-hemolysin variants and uses thereof
CN115052882B (zh) 2020-02-06 2024-05-24 豪夫迈·罗氏有限公司 减少模板穿入纳米孔的组合物
ES2999861T3 (en) 2020-08-11 2025-02-27 Hoffmann La Roche Nucleoside-5'-oligophosphates tagged with positively-charged polymers, nanopores incorporating negative charges, and methods and systems using the same
WO2022263496A1 (en) 2021-06-17 2022-12-22 F. Hoffmann-La Roche Ag Engineered nanopore with a negatively charged polymer threaded through the channel
WO2022263489A1 (en) 2021-06-17 2022-12-22 F. Hoffmann-La Roche Ag Nucleoside-5 -oligophosphates having a cationically-modified nucleobase
EP4373925A1 (en) 2021-07-21 2024-05-29 F. Hoffmann-La Roche AG Alpha-hemolysin variants forming narrow channel pores and uses thereof
EP4437090A1 (en) 2021-11-22 2024-10-02 F. Hoffmann-La Roche AG Thermostable polymerase variants
WO2026050292A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Optimized reagent introduction during a nanopore sequencing assay
WO2026050290A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Lipid composition and methods of use in a nanopore sequencing assay
WO2026050289A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Pre-and post-sequencing cleaning protocols for nanopore sequencing

Citations (2)

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CN1218832A (zh) * 1997-09-11 1999-06-09 霍夫曼—拉罗奇有限公司 用于测序的改变的热稳定dna聚合酶
CN102245761A (zh) * 2008-11-03 2011-11-16 卡帕生物系统 修饰a型dna聚合酶

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EP1888743B1 (en) 2005-05-10 2011-08-03 Illumina Cambridge Limited Improved polymerases
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WO2012083249A2 (en) 2010-12-17 2012-06-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
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CN102245761A (zh) * 2008-11-03 2011-11-16 卡帕生物系统 修饰a型dna聚合酶

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Also Published As

Publication number Publication date
US11788073B2 (en) 2023-10-17
JP2019187424A (ja) 2019-10-31
WO2016183403A2 (en) 2016-11-17
CA2978194A1 (en) 2016-11-17
EP3294749A2 (en) 2018-03-21
US20160333327A1 (en) 2016-11-17
US20200087636A1 (en) 2020-03-19
CA3028797C (en) 2023-01-03
US10526588B2 (en) 2020-01-07
US10968438B2 (en) 2021-04-06
CA2978194C (en) 2021-11-16
US20210189355A1 (en) 2021-06-24
WO2016183403A3 (en) 2017-01-05
JP2018515103A (ja) 2018-06-14
JP2021121221A (ja) 2021-08-26
EP3294749A4 (en) 2018-10-31
CA3028797A1 (en) 2016-11-17

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