JP2018515103A - ポリメラーゼバリアントおよびその使用 - Google Patents

ポリメラーゼバリアントおよびその使用 Download PDF

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Publication number
JP2018515103A
JP2018515103A JP2017559318A JP2017559318A JP2018515103A JP 2018515103 A JP2018515103 A JP 2018515103A JP 2017559318 A JP2017559318 A JP 2017559318A JP 2017559318 A JP2017559318 A JP 2017559318A JP 2018515103 A JP2018515103 A JP 2018515103A
Authority
JP
Japan
Prior art keywords
polymerase
modified
nucleotide
dna polymerase
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017559318A
Other languages
English (en)
Japanese (ja)
Inventor
エイヤー，アルナ
シュワブ，チャールズ
アーノルド，クレオマ
レダーマン，イリア
タイ，アイリーン
シュルツ，タイラー・オブライエン
マクガー，コリン・アレクサンダー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/012,317 external-priority patent/US10308918B2/en
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2018515103A publication Critical patent/JP2018515103A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2017559318A 2015-05-14 2016-05-13 ポリメラーゼバリアントおよびその使用 Pending JP2018515103A (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201562161571P 2015-05-14 2015-05-14
US62/161,571 2015-05-14
US201562202895P 2015-08-09 2015-08-09
US62/202,895 2015-08-09
US15/012,317 US10308918B2 (en) 2015-02-02 2016-02-01 Polymerase variants
US15/012,317 2016-02-01
US15/151,364 US10526588B2 (en) 2015-05-14 2016-05-10 Polymerase variants and uses thereof
US15/151,364 2016-05-10
PCT/US2016/032258 WO2016183403A2 (en) 2015-05-14 2016-05-13 Polymerase variants and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019104273A Division JP2019187424A (ja) 2015-05-14 2019-06-04 ポリメラーゼバリアントおよびその使用

Publications (1)

Publication Number Publication Date
JP2018515103A true JP2018515103A (ja) 2018-06-14

Family

ID=57248698

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2017559318A Pending JP2018515103A (ja) 2015-05-14 2016-05-13 ポリメラーゼバリアントおよびその使用
JP2019104273A Pending JP2019187424A (ja) 2015-05-14 2019-06-04 ポリメラーゼバリアントおよびその使用
JP2021086806A Pending JP2021121221A (ja) 2015-05-14 2021-05-24 ポリメラーゼバリアントおよびその使用

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2019104273A Pending JP2019187424A (ja) 2015-05-14 2019-06-04 ポリメラーゼバリアントおよびその使用
JP2021086806A Pending JP2021121221A (ja) 2015-05-14 2021-05-24 ポリメラーゼバリアントおよびその使用

Country Status (6)

Country Link
US (3) US10526588B2 (https=)
EP (1) EP3294749A4 (https=)
JP (3) JP2018515103A (https=)
CN (1) CN107636004A (https=)
CA (2) CA2978194C (https=)
WO (1) WO2016183403A2 (https=)

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JP2019512217A (ja) * 2016-02-29 2019-05-16 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ

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JP6736565B2 (ja) 2015-02-02 2020-08-05 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ポリメラーゼバリアント
US10526588B2 (en) * 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
US10745685B2 (en) 2015-11-16 2020-08-18 Stratos Genomics, Inc. DP04 polymerase variants
EP3423574B1 (en) * 2016-02-29 2021-03-17 Genia Technologies, Inc. Polymerase-template complexes for nanopore sequencing
US10590480B2 (en) 2016-02-29 2020-03-17 Roche Sequencing Solutions, Inc. Polymerase variants
US10655174B2 (en) 2016-05-27 2020-05-19 Roche Sequencing Solutions, Inc. Tagged multi-nucleotides useful for nucleic acid sequencing
US10669580B2 (en) 2016-08-26 2020-06-02 Roche Sequencing Solutions, Inc. Tagged nucleotides useful for nanopore detection
US10829747B2 (en) 2016-09-22 2020-11-10 Roche Sequencing Solutions, Inc. Polymerase variants
WO2018204707A1 (en) 2017-05-04 2018-11-08 Stratos Genomics Inc. Dp04 polymerase variants
CA3084812A1 (en) 2017-12-11 2019-06-20 Stratos Genomics, Inc. Dpo4 polymerase variants with improved accuracy
WO2019166457A1 (en) 2018-02-28 2019-09-06 F. Hoffmann-La Roche Ag Tagged nucleoside compounds useful for nanopore detection
CN112041331B (zh) 2018-02-28 2024-05-28 豪夫迈·罗氏有限公司 α-溶血素变体及其用途
WO2021156370A1 (en) 2020-02-06 2021-08-12 F. Hoffmann-La Roche Ag Compositions that reduce template threading into a nanopore
EP4196608B1 (en) 2020-08-11 2024-10-23 F. Hoffmann-La Roche AG Nucleoside-5'-oligophosphates tagged with positively-charged polymers, nanopores incorporating negative charges, and methods and systems using the same
EP4355757A1 (en) 2021-06-17 2024-04-24 F. Hoffmann-La Roche AG Nucleoside-5 -oligophosphates having a cationically-modified nucleobase
US20250283162A1 (en) 2021-06-17 2025-09-11 Roche Sequencing Solutions, Inc. Engineered nanopore with a negatively charged polymer threaded through the channel
CN117999346A (zh) 2021-07-21 2024-05-07 豪夫迈·罗氏有限公司 形成窄通道孔的α-溶血素变体及其用途
WO2023089175A1 (en) 2021-11-22 2023-05-25 F. Hoffmann-La Roche Ag Thermostable polymerase variants
WO2026050292A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Optimized reagent introduction during a nanopore sequencing assay
WO2026050290A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Lipid composition and methods of use in a nanopore sequencing assay
WO2026050289A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Pre-and post-sequencing cleaning protocols for nanopore sequencing

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2016124543A1 (en) * 2015-02-02 2016-08-11 F. Hoffmann-La Roche Ag Polymerase variants

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA2243985C (en) 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2006120433A1 (en) 2005-05-10 2006-11-16 Solexa Limited Improved polymerases
GB0509508D0 (en) 2005-05-10 2005-06-15 Solexa Ltd Improved polymerases
WO2009122191A1 (en) 2008-04-04 2009-10-08 It-Is International Ltd Thermal control system and method for chemical and biochemical reactions
CA2742594C (en) * 2008-11-03 2020-03-24 Kapa Biosystems, Inc. Modified type a dna polymerases
CA2995178C (en) 2010-02-08 2023-09-12 Genia Technologies, Inc. Method and system for forming a nanopore in a lipid bilayer
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EP2652153B1 (en) 2010-12-17 2017-07-05 The Trustees of Columbia University in the City of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
US10059984B2 (en) 2011-06-16 2018-08-28 The Regents Of The University Of California Salt-tolerant DNA polymerases
CN103857805B (zh) 2011-08-10 2017-07-18 生命技术公司 聚合酶组合物、制备和使用所述聚合酶组合物的方法
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JP2016171215A (ja) 2015-03-12 2016-09-23 株式会社東芝 半導体記憶装置
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WO2017148862A1 (en) 2016-02-29 2017-09-08 Genia Technologies, Inc. Polymerase variants
CN108699539B (zh) 2016-02-29 2022-11-18 吉尼亚科技公司 外切核酸酶缺陷的聚合酶

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WO2016124543A1 (en) * 2015-02-02 2016-08-11 F. Hoffmann-La Roche Ag Polymerase variants

Non-Patent Citations (1)

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PLOS ONE, vol. Vol.7, Issue 5, JPN6018051901, 2012, pages 38283, ISSN: 0003952270 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019512217A (ja) * 2016-02-29 2019-05-16 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ

Also Published As

Publication number Publication date
US10526588B2 (en) 2020-01-07
US10968438B2 (en) 2021-04-06
US20160333327A1 (en) 2016-11-17
US11788073B2 (en) 2023-10-17
WO2016183403A3 (en) 2017-01-05
EP3294749A4 (en) 2018-10-31
CA2978194A1 (en) 2016-11-17
JP2021121221A (ja) 2021-08-26
CA2978194C (en) 2021-11-16
CA3028797C (en) 2023-01-03
JP2019187424A (ja) 2019-10-31
EP3294749A2 (en) 2018-03-21
CN107636004A (zh) 2018-01-26
US20200087636A1 (en) 2020-03-19
CA3028797A1 (en) 2016-11-17
US20210189355A1 (en) 2021-06-24
WO2016183403A2 (en) 2016-11-17

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