CN107616439A - A kind of technique for being enriched with Holothurian machining liquid nutritional ingredient - Google Patents
A kind of technique for being enriched with Holothurian machining liquid nutritional ingredient Download PDFInfo
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- CN107616439A CN107616439A CN201710966763.5A CN201710966763A CN107616439A CN 107616439 A CN107616439 A CN 107616439A CN 201710966763 A CN201710966763 A CN 201710966763A CN 107616439 A CN107616439 A CN 107616439A
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- holothurian machining
- machining liquid
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Abstract
The invention discloses a kind of technique for being enriched with Holothurian machining liquid nutritional ingredient, NaHCO is added after Holothurian machining liquid is filtered, centrifuged3And Na2CO3And mixed solution, 0.2 0.5moL/L HCl solution is then added, is finally placed in PDMS membrane bioreactors, pervaporation is carried out, obtains concentrate;Concentrate is sterilized using pasteurization methods, is then refrigerated at 4 DEG C standby.By PROCESS FOR TREATMENT Holothurian machining liquid provided by the invention can not only enriching and recovering utilize nutritional ingredient in Holothurian machining liquid, and Holothurian machining liquid after treatment, the liquid of discharge are the vapor condensed out, reduce the pollution to surrounding environment.
Description
Technical field
The present invention relates to a kind of Holothurian machining liquid processing method, more particularly to it is a kind of to Holothurian machining liquid business into separating/enriching
The technique of recycling.
Background technology
Holothurian machining liquid is the waste liquid in sea cucumber transport process, during sea cucumber is soaked, due to the self-dissolving of sea cucumber
Reason, cause the nutrient loss such as protein, polysaccharide and amino acid in sea cucumber into soak, thus Holothurian machining liquid
In be rich in the various nutritional ingredients beneficial to human body, some component contents have been even more than the sea cucumber soaked in itself.It is but right
All it is directly to discard mostly in the processing mode of Holothurian machining liquid, not only causes the loss of nutriment, and pollute
Surrounding environment.
The content of the invention
The defects of in order to overcome environmental pollution caused by existing Holothurian machining liquid directly discards and nutrition to waste, the present invention
A kind of technique for being enriched with Holothurian machining liquid nutritional ingredient is provided, can not only enriching and recovering utilization after the PROCESS FOR TREATMENT
Nutritional ingredient in Holothurian machining liquid, and the downstream water of concentration out can be expelled directly out, and will not also cause the dirt to environment
Dye.
In order to solve the above technical problems, the technical solution adopted in the present invention is:
A kind of technique for being enriched with Holothurian machining liquid nutritional ingredient, it is characterised in that:Comprise the technical steps that
Step 1, Holothurian machining liquid is removed into the solid and suspension in waste liquid by plate and frame filter;
Step 2, liquid step 1 treated, is placed in a centrifuge carry out centrifugal treating, centrifugation time is 20~40 min;
Step 3, NaHCO is added in the liquid treated to step 23And Na2CO3And mixed solution, the concentration of mixed solution are:
0.1~0.25 moL/L, the amount of addition are:Mixed solution and the volume ratio of liquid of processing are: 1:(10~25), during reaction
Between be 20-40 min, be filtrated to get liquid;
Step 4,0.2~0.50 moL/L HCl solution is added into the liquid in step 3, the amount of addition is:HCL solution with
The volume ratio of the liquid of processing is: 1:(20~50), liquid is then heated to 35~55 DEG C again, stirs 26~40 min;
Step 5, the liquid after step 4 is handled is placed in PDMS(Silicon rubber)In membrane bioreactor, pervaporation is carried out,
Obtain concentrate;
Step 6, the concentrate after step 5 processing is sterilized using pasteurization methods, refrigerated at 4 DEG C standby.
In step 5, in PDMS(Silicon rubber)In membrane bioreactor, the temperature control of upstream Holothurian machining liquid is 28~40
DEG C, the rotating speed control of agitating paddle is 120~180 r/min, and working fluid flows to downstream membrane device, film with 160~220L/h speed
The vacuum degree control in downstream is 0.8~1.5 kPa, condensation temperature of the vapor that downstream sub-argument comes out at -4~-10 DEG C
Lower condensation.
Increase removes cationic step after step 4, by entering back into step 5 afterwards except cationic step, except cation
Step is specially:The liquid after step 4 is handled with amberplex, except decationizing, the liquid after processing enters
Step 5.
The rotating speed control of centrifuge is 5000 r/min, centrifugation time 30min in step 2.
NaHCO in step 33And Na2CO3The concentration of mixed solution is 0.1 moL/L, NaHCO in mixed liquor3Solution with
Na2CO3Solution mole is:1:9.
In step 4, the concentration of HCl solution is 0.2moL/L, and heating-up temperature is 45 DEG C, mixing time 30min.
In step 5, in PDMS(Silicon rubber)In membrane bioreactor, the temperature control of upstream Holothurian machining liquid at 35 DEG C,
The rotating speed control of agitating paddle is 150 r/min, and working fluid flows to downstream membrane device, the vacuum control in film downstream with 200L/h speed
It is made as 1000 Pa.
The present invention has the advantages that relative to prior art:
1st, the present invention, by Holothurian machining liquid by plate and frame filter, removes the solid and suspension in waste liquid by step 1;
Step 2, liquid step 1 treated, carry out centrifugal treating is placed in a centrifuge, centrifugation time is 20~40 minutes, centrifugation
Processing removes the fungal cells such as macromolecular substances and the saccharomycete in liquid;Step 3, add in the liquid treated to step 2
Enter NaHCO3And Na2CO3And mixed solution, remove the Ca in liquid2+、Mg2+、Cu2+Plasma;Step 4, to the liquid in step 3
HCl solution is added in body, liquid is then heated to 35~55 DEG C again, stirs 26~40min, removes the HCO in solution3 —From
Son;Step 5, the liquid after step 4 is handled is placed in PDMS(Silicon rubber)In membrane bioreactor, pervaporation is carried out,
Obtain concentrate;Pass through PDMS(Silicon rubber)PDMS membrane filtrations in membrane bioreactor remove the moisture in liquid, leave egg
The nutritional ingredients such as white matter, polysaccharide and amino acid, the concentrate of these nutritional ingredients is obtained, to use, removed using UF membrane
Moisture concentrating spent liquor is removed, for existing concentration technique, it is concentrated because temperature is than relatively low(28~40 DEG C), so energy
Enough the bioactivity of material is kept not change.Step 6, the concentrate after step 5 processing is entered using pasteurization methods
Row sterilization, the concentrate after sterilization turn into safe and non-toxic nutritional concentrated solution, are easy to later-stage utilization.Pass through the place of above-mentioned processing step
Reason and control, could be recycled, the water of discharge is vapor, to surrounding environment to the nutritional ingredient in Holothurian machining liquid
Without any harm, the purpose of energy-saving and emission-reduction is reached.
2nd, in steps of 5, in PDMS(Silicon rubber)In membrane bioreactor, the temperature control of upstream Holothurian machining liquid is 28
~40 DEG C, the rotating speed control of agitating paddle is 120~180 r/min, and working fluid flows to downstream film with 160~220L/h speed
Device, the vacuum degree control in film downstream are 0.8~1.5 kPa, condensation of the vapor that downstream sub-argument comes out at -4~-10 DEG C
Condensed out in device.By the control of these parameters, ensure PDMS(Silicon rubber)Membrane bioreactor can be carried out most to liquid
The concentration of big degree, maximum recovery is carried out to the nutritional ingredient in Holothurian machining liquid, filtering is thorough, filter efficiency
Height, and temperature control completes liquid concentration at a lower temperature than relatively low, can fully ensure that the bioactivity of material is not sent out
It is raw to change.
3rd, after step 4 increase remove cationic step, by except cationic step afterwards enter back into step 5, except sun from
Sub-step is specially:The liquid after step 4 is handled with amberplex, obtained liquid enters step 5.Pass through this
The effect of step, harmful heavy metal ion can be particularly to the cation in Holothurian machining liquid and handled, purify liquid
In harmful substance, reduce the pollution to surrounding environment.
4th, the rotating speed control of centrifuge is 5000 r/min in step 2, centrifugation time 30min, NaHCO in step 33With
Na2CO3The concentration of mixed solution is 0.1 moL/L, NaHCO3And Na2CO3NaHCO in mixed liquor3Solution and Na2CO3Solution rubs
You are:1:9, in step 4, the concentration of HCl solution is 0.2 moL/L, and heating-up temperature is 45 DEG C, mixing time 30min, step
In 5, in PDMS(Silicon rubber)In membrane bioreactor, the temperature control of upstream Holothurian machining liquid is at 35 DEG C, the rotating speed of agitating paddle
Control and downstream membrane device is flowed to 200L/h speed for 150 r/min, working fluid, the vacuum degree control in film downstream is 1000Pa.
Specifically controlled by the synthesis of parameter, ensure that the harmful substance in Holothurian machining liquid can be got rid of, obtained concentrate safety
Nontoxic, nutritional ingredient is high, recycles value greatly, the liquid of discharge is the vapor condensed out, is reduced to surrounding environment
Pollution.
Embodiment
Embodiment 1
Present embodiments provide a kind of technique for being enriched with Holothurian machining liquid nutritional ingredient, it is characterised in that:Walked including following technique
Suddenly
Step 1, Holothurian machining liquid is removed into the solid and suspension in waste liquid by plate and frame filter;
Step 2, liquid step 1 treated, carry out centrifugal treating is placed in a centrifuge, centrifugation time is 20 minutes, centrifugation
Speed is 4800r/min;
Step 3, NaHCO is added in the liquid treated to step 23And Na2CO3And mixed solution, the concentration of mixed solution are:
0.1moL/L, the volume ratio of liquid of the mixed solution of addition with handling are:1:10, the reaction time is 20 min, is filtrated to get
Liquid;
Step 4,0.1moL/L HCl solution is added into the liquid in step 3, the amount of addition is 1:20
, liquid is then heated to 35 DEG C again, stirs 40min;
Step 5, the liquid after step 4 is handled is placed in PDMS(Silicon rubber)In membrane bioreactor, pervaporation is carried out,
Obtain concentrate;
Step 6, the concentrate after step 5 processing is sterilized using pasteurization methods, refrigerated at 4 DEG C standby.
In step 5, in PDMS(Silicon rubber)In membrane bioreactor, the temperature control of upstream Holothurian machining liquid at 40 DEG C,
The rotating speed control of agitating paddle is 180 r/min, and working fluid flows to downstream membrane device, the vacuum control in film downstream with 160L/h speed
0.8kPa is made as, the vapor that downstream sub-argument comes out condenses under the condensation temperature at -4 DEG C.
Increase removes cationic step after step 4, by entering back into step 5 afterwards except cationic step, except cation
Step is specially:The liquid after step 4 is handled with amberplex, except decationizing, the liquid after processing enters
Step 5.
Before concentration, in Holothurian machining liquid, the content of crude protein is 284.9mg/L, Thick many candies 2.91mg/L, after concentration in
The content of crude protein is 854.7 mg/L, Thick many candies content is 8.73 mg/L, lead before concentration(Pb)Content be 1.2mg/kg,
Chromium(Cd)Content be 0.15 mg/kg, mercury(Hg)Content be 0.8 mg/kg, lead after concentration(Pb)Content be 0.82mg/
Kg, chromium(Cd)Content be 0.08mg/kg, mercury(Hg)Content be 0.42mg/kg.
Embodiment 2
A kind of technique for being enriched with Holothurian machining liquid nutritional ingredient, it is characterised in that:Comprise the technical steps that
Step 1, Holothurian machining liquid is removed into the solid and suspension in waste liquid by plate and frame filter;
Step 2, liquid step 1 treated, carry out centrifugal treating is placed in a centrifuge, centrifugation time is 25 minutes, centrifugation
Speed is 4200r/min;
Step 3, NaHCO is added in the liquid treated to step 23And Na2CO3And mixed solution, the concentration of mixed solution are:
0.11 moL/L, the volume ratio of liquid of the mixed solution of addition with handling are:1:15, the reaction time is 30 min, is filtered
To liquid;
Step 4,0.2moL/L HCl solution is added into the liquid in step 3, the amount of addition is:HCL solution and the liquid of processing
The volume ratio of body is:1:25, liquid is then heated to 40 DEG C again, stirs 30min;
Step 5, the liquid after step 4 is handled is placed in PDMS(Silicon rubber)In membrane bioreactor, pervaporation is carried out,
Obtain concentrate;
Step 6, the concentrate after step 5 processing is sterilized using pasteurization methods, refrigerated at 4 DEG C standby.
In step 5, in PDMS(Silicon rubber)In membrane bioreactor, the temperature control of upstream Holothurian machining liquid at 32 DEG C,
The rotating speed control of agitating paddle is 160 r/min, and working fluid flows to downstream membrane device, the vacuum control in film downstream with 165L/h speed
1.2 kPa are made as, the vapor that downstream sub-argument comes out condenses under the condensation temperature at -6 DEG C.
Increase removes cationic step after step 4, by entering back into step 5 afterwards except cationic step, except cation
Step is specially:The liquid after step 4 is handled with amberplex, except decationizing, the liquid after processing enters
Step 5.
Before concentration, in Holothurian machining liquid, the content of crude protein is 284.9mg/L, Thick many candies 2.91mg/L, after concentration in
The content of crude protein is 1424.5 mg/L, Thick many candies content is 14.55 mg/L, lead before concentration(Pb)Content be 1.2mg/
Kg, chromium(Cd)Content be 0.15 mg/kg, mercury(Hg)Content be 0.8 mg/kg, lead after concentration(Pb)Content be
0.085mg/kg, chromium(Cd)Content be 0.09mg/kg, mercury(Hg)Content be 0.48mg/kg.
Embodiment 3
A kind of technique for being enriched with Holothurian machining liquid nutritional ingredient, it is characterised in that:Comprise the technical steps that
Step 1, Holothurian machining liquid is removed into the solid and suspension in waste liquid by plate and frame filter;
Step 2, liquid step 1 treated, is placed in a centrifuge carry out centrifugal treating, and centrifugation time is 35 min, centrifugation
Speed is 5200r/min;
Step 3, NaHCO is added in the liquid treated to step 23And Na2CO3And mixed solution, the concentration of mixed solution are:
0.18 moL/L, the volume ratio of liquid of the mixed solution of addition with handling are:1:20, the reaction time is that 35 min are filtrated to get
Liquid;
Step 4,0.35moL/L HCl solution is added into the liquid in step 3, the amount of addition is HCL solution and the liquid of processing
The volume ratio of body is:1:35, liquid is then heated to 40 DEG C again, stirs 32min;
Step 5, the liquid after step 4 is handled is placed in PDMS(Silicon rubber)In membrane bioreactor, pervaporation is carried out,
Obtain concentrate;
Step 6, the concentrate after step 5 processing is sterilized using pasteurization methods, refrigerated at 4 DEG C standby.
In step 5, in PDMS(Silicon rubber)In membrane bioreactor, the temperature control of upstream Holothurian machining liquid at 32 DEG C,
The rotating speed control of agitating paddle is 175 r/min, and working fluid flows to downstream membrane device, the vacuum control in film downstream with 195L/h speed
1.35 kPa are made as, the vapor that downstream sub-argument comes out condenses under the condensation temperature at -8 DEG C.
Increase removes cationic step after step 4, by entering back into step 5 afterwards except cationic step, except cation
Step is specially:The liquid after step 4 is handled with amberplex, except decationizing, the liquid after processing enters
Step 5.
Before concentration, in Holothurian machining liquid, the content of crude protein is 284.9mg/L, Thick many candies 2.91mg/L, after concentration in
The content of crude protein is 2279.2mg/L, Thick many candies content is 23.28mg/L, lead before concentration(Pb)Content be 1.2mg/kg,
Chromium(Cd)Content be 0.15 mg/kg, mercury(Hg)Content be 0.8 mg/kg, lead after concentration(Pb)Content be 0.75mg/
Kg, chromium(Cd)Content be 0.078mg/kg, mercury(Hg)Content be 0.46 mg/kg.
Embodiment 4
A kind of technique for being enriched with Holothurian machining liquid nutritional ingredient, it is characterised in that:Comprise the technical steps that
Step 1, Holothurian machining liquid is removed into the solid and suspension in waste liquid by plate and frame filter;
Step 2, liquid step 1 treated, carry out centrifugal treating is placed in a centrifuge, centrifugation time is 40 minutes, centrifugation
Speed is 5000 r/min;
Step 3, NaHCO is added in the liquid treated to step 23And Na2CO3And mixed solution, the concentration of mixed solution are:
0.1 moL/L, the amount of addition are:Mixed solution and the volume ratio of liquid of processing are: 1:25, the reaction time is 40 min,
It is filtrated to get liquid;
It is filtrated to get liquid;
Step 4,0.2moL/L HCl solution is added into the liquid in step 3, the amount of addition is HCL solution and the liquid of processing
The volume ratio of body is:1:50, liquid is then heated to 45 DEG C again, stirs 30min;
Step 5, the liquid after step 4 is handled is placed in PDMS(Silicon rubber)In membrane bioreactor, pervaporation is carried out,
Obtain concentrate;
Step 6, the concentrate after step 5 processing is sterilized using pasteurization methods, refrigerated at 4 DEG C standby.
In step 5, in PDMS(Silicon rubber)In membrane bioreactor, the temperature control of upstream Holothurian machining liquid at 35 DEG C,
The rotating speed control of agitating paddle is 150 r/min, and working fluid flows to downstream membrane device, the vacuum control in film downstream with 200L/h speed
Be made as 1.0kPa, the vapor that downstream sub-argument comes out DEG C at condenser in condense out.
Increase removes cationic step after step 4, by entering back into step 5 afterwards except cationic step, except cation
Step is specially:The liquid after step 4 is handled with amberplex, except decationizing, the liquid after processing enters
Step 5.
Before concentration, in Holothurian machining liquid, the content of crude protein is 284.9mg/L, Thick many candies 2.91mg/L, after concentration in
The content of crude protein is 2849 mg/ L, Thick many candies content is 29.1 mg/ L, lead before concentration(Pb)Content be 1.2mg/kg,
Chromium(Cd)Content be 0.15 mg/kg, mercury(Hg)Content be 0.8 mg/kg, lead after concentration(Pb)Content be 0.74mg/
Kg, chromium(Cd)Content be 0.06mg/kg, mercury(Hg)Content be 0.38mg/kg.
Embodiment 5
A kind of technique for being enriched with Holothurian machining liquid nutritional ingredient, it is characterised in that:Comprise the technical steps that
Step 1, Holothurian machining liquid is removed into the solid and suspension in waste liquid by plate and frame filter;
Step 2, liquid step 1 treated, carry out centrifugal treating being placed in a centrifuge, centrifugation time is 20~40 minutes,
Centrifugal speed is 5600r/min;
Step 3, NaHCO is added in the liquid treated to step 23And Na2CO3And mixed solution, the concentration of mixed solution are:
0.25 moL/L, the amount of addition are that the volume ratio of mixed solution and the liquid of processing is: 1:18, the reaction time is 38 min, mistake
Filter obtains liquid;
Step 4,0.32moL/L HCl solution is added into the liquid in step 3, the amount of addition is HCL solution and the liquid of processing
The volume ratio of body is:1:45, liquid is then heated to 55 DEG C again, stirs 40min;
Step 5, the liquid after step 4 is handled is placed in PDMS(Silicon rubber)In membrane bioreactor, pervaporation is carried out,
Obtain concentrate;
Step 6, the concentrate after step 5 processing is sterilized using pasteurization methods, refrigerated at 4 DEG C standby.
In step 5, in PDMS(Silicon rubber)In membrane bioreactor, the temperature control of upstream Holothurian machining liquid at 40 DEG C,
The rotating speed control of agitating paddle is 180 r/min, and working fluid flows to downstream membrane device, the vacuum control in film downstream with 220L/h speed
Be made as 1.5 kPa, the vapor that downstream sub-argument comes out DEG C at condenser in condense out.
Increase removes cationic step after step 4, by entering back into step 5 afterwards except cationic step, except cation
Step is specially:The liquid after step 4 is handled with amberplex, except decationizing, the liquid after processing enters
Step 5.
Before concentration, in Holothurian machining liquid, the content of crude protein is 284.9mg/L, Thick many candies 2.91mg/L, after concentration in
The content of crude protein is 1709.4 mg/ L, Thick many candies content is 17.46mg/L, lead before concentration(Pb)Content be 1.2 mg/
Kg, chromium(Cd)Content be 0.15 mg/kg, mercury(Hg)Content be 0.8 mg/kg, lead after concentration(Pb)Content be
0.84mg/kg, chromium(Cd)Content be 0.85mg/kg, mercury(Hg)Content be 0.48mg/kg.
Claims (7)
- A kind of 1. technique for being enriched with Holothurian machining liquid nutritional ingredient, it is characterised in that:Comprise the technical steps thatStep 1, Holothurian machining liquid is removed into the solid and suspension in waste liquid by plate and frame filter;Step 2, liquid step 1 treated, is placed in a centrifuge carry out centrifugal treating, and centrifugation time is 20~40 minutes;Step 3, NaHCO is added in the liquid treated to step 23And Na2CO3And mixed solution, the concentration of mixed solution are: 0.1~0.25 moL/L, the amount of addition are:Mixed solution and the volume ratio of liquid of processing are: 1:(10~25), during reaction Between be 20-40 min, be filtrated to get liquid;Step 4,0.2~0.50 moL/L HCl solution is added into the liquid in step 3, the amount of addition is:HCL solution with The volume ratio of the liquid of processing is: 1:(20~50), liquid is then heated to 35~55 DEG C again, stirs 26~40min;Step 5, the liquid after step 4 is handled is placed in PDMS membrane bioreactors, carries out pervaporation, concentrated Liquid;Step 6, the concentrate after step 5 processing is sterilized using pasteurization methods, refrigeration is standby.
- A kind of 2. technique for being enriched with Holothurian machining liquid nutritional ingredient according to claim 1, it is characterised in that:In step 5, In PDMS membrane bioreactors, for the temperature control of upstream Holothurian machining liquid at 28~40 DEG C, the rotating speed control of agitating paddle is 120 ~180 r/min, working fluid flow to downstream membrane device with 160~220L/h speed, the vacuum degree control in film downstream for 0.8~ 1.5 kPa, the vapor that downstream sub-argument comes out condense under the condensation temperature at -4~-10 DEG C.
- A kind of 3. technique for being enriched with Holothurian machining liquid nutritional ingredient according to claim 1 or 2, it is characterised in that:In step Increase removes cationic step after rapid 4, by entering back into step 5 afterwards except cationic step, except cationic step is specially:With Amberplex is handled the liquid after step 4, and except decationizing, the liquid after processing enters step 5.
- A kind of 4. technique for being enriched with Holothurian machining liquid nutritional ingredient according to claim 1, it is characterised in that:In step 2 The rotating speed control of centrifuge is 5000 r/min, centrifugation time 30min.
- A kind of 5. technique for being enriched with Holothurian machining liquid nutritional ingredient according to claim 1, it is characterised in that:In step 3 NaHCO3And Na2CO3The concentration of mixed solution is 0.1 moL/L, NaHCO in mixed liquor3Solution and Na2CO3Solution mole is: 1:9。
- A kind of 6. technique for being enriched with Holothurian machining liquid nutritional ingredient according to claim 1, it is characterised in that:In step 4, The concentration of HCl solution is 0.2 moL/L, and heating-up temperature is 45 DEG C, mixing time 30min.
- A kind of 7. technique for being enriched with Holothurian machining liquid nutritional ingredient according to claim 1, it is characterised in that:In step 5, In PDMS membrane bioreactors, for the temperature control of upstream Holothurian machining liquid at 35 DEG C, the rotating speed control of agitating paddle is 150 r/ Min, working fluid flow to downstream membrane device with 200L/h speed, and the vacuum degree control in film downstream is 1000Pa.
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CN109498658A (en) * | 2019-01-07 | 2019-03-22 | 哈尔滨工业大学(威海) | A kind of Holothurian machining liquid active principle extracting method |
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