CN107616355A

CN107616355A - The preparation method of grouper nutrition fortifier

Info

Publication number: CN107616355A
Application number: CN201710732791.0A
Authority: CN
Inventors: 陈义娜
Original assignee: Pujiang County Cooperative Animal Feed Technology Development Co Ltd
Current assignee: Pujiang County Cooperative Animal Feed Technology Development Co Ltd
Priority date: 2017-08-24
Filing date: 2017-08-24
Publication date: 2018-01-23

Abstract

The invention discloses a kind of preparation method of grouper nutrition fortifier, including：The preparation of Chinese medicine preparation, the preparation of liposome, extraction fish guts oil, fish oil modification, compounding hardening agent.Have the beneficial effect that：Chinese medicine preparation in grouper nutrition fortifier can strengthen the disease-resistant antibacterial ability of grouper, improve immunity of organisms, build up health；Rich in polyunsaturated fatty acids such as DHA, EPA in modified fish guts oil, the nutrition fortifier of grouper is suitable as, after being fed by grouper, Modified Fish Oil can improve the lipid structure in grouper body；Contain the nutritional ingredients such as abundant traditional Chinese medicine ingredients, phosphatide, unrighted acid, protein, complex enzyme, mineral element in hardening agent, phagostimulant is coordinated to support chemical conversion point with anti-, it is suitable as the nutrition fortifier of grouper, after being eaten with feed formulation, stress ability, immunocompetence, the disease-resistant antibacterial ability of grouper can be strengthened, promote it to digest and assimilate nutriment, accelerate growing for grouper.

Description

The preparation method of grouper nutrition fortifier

Technical field

The invention belongs to aquatic biological nutrient laden hardening agent field, more particularly to a kind of preparation of grouper nutrition fortifier Method.

Technical background

Grouper, belong to Perciformes, body oblong is slightly flat-sided, and mouth is big, has supplemental maxillary, tooth apicule, and some is expanded into dog Tooth, body are had and are embedded in often subcutaneous by small ctenoid scale, and dorsal fin and stern fin spine are flourishing, and tail fin is circular or spill, and variation in body color is a lot of, often It is brown or red, and have striped and spot, it is the big-and-middle-sized seafood fish of water warm.Lithosporic fish body ellipse, flat-sided, head Greatly, kiss is short and blunt circle, mouth are big, and have prosperity spreads bone, and body drapes over one's shoulders tiny ctenoid scale, and dorsal fin is powerful, and body colour can change with environmental change Become.Into fish length generally at 20~30 centimetres.Grouper is nutritious, and fine and tender taste is pure white, similar chicken, is known as " extra large chicken " Title.Grouper is a kind of low fat, the first-class food fish of high protein, is pushed away by Hong Kong and Macao as one of four big fishes of China.Stone The content of spot fish protein is high, and fat content is low, in addition to the amino acid necessary to containing body metabolism, also rich in plurality of inorganic salt With iron, calcium, phosphorus and various vitamins.The nutritional ingredient of fish-skin colloid, to strengthening the complete growth of epithelial tissue and promoting glue The synthesis of archaeocyte plays an important role, and is referred to as the fish of beauty and skin care, is especially suitable for women and eats in postpartum.Grouper nutritive value It is very high, rich in nutritional ingredients such as protein, vitamin A, vitamin D, calcium, phosphorus, potassium.

Technology of preparing on the feed of grouper has many methods, and prior art is as applied for public number

The B of CN 101803673 Chinese invention patent, discloses a kind of grouper compound feed and preparation method thereof, the invention Method is by fish meal, dregs of beans, wheat Strong flour, flour, dusty yeast, B B-complex, composite mineral matter, choline, biphosphate Calcium, gelatin, VC, shrimp powder are mixed with feed, have and prepare the advantages of simple, nutrition is comprehensive, but its primary raw material fish meal Expensive, scale application improves the cost of feed, and lacks fat constituent in feed with improving the medicine of grouper Composition, unleavened dregs of beans contain anti-nutrition component, have impact on grouper and feed nutrition is assimilated.

The content of the invention

The purpose of this method is to provide a kind of preparation method of grouper nutrition fortifier, and the preparation method is simply easy Control, nutrition fortifier is nutritious, can effectively improve grouper immunity of organisms, promote it to grow.

The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：The system of grouper nutrition fortifier Preparation Method, including：The preparation of Chinese medicine preparation, the preparation of liposome, extraction fish guts oil, fish oil modification, compounding hardening agent, specifically Comprise the following steps：

The preparation of Chinese medicine preparation：By 10-15 parts coptis, 3-5 parts fleece-flower root, 5-8 parts golden cypress and 10-15 parts mast respectively with crushing Machine crushes, and crosses 80-100 mesh sieves, compound Chinese medicinal preparation is made, weighs 10-12 part compound Chinese medicinal preparations, is wrapped with degreasing cotton yarn, Add in 100-120 part water, it is boiled after immersion 1-2 hours, 30-40 minutes are cooked by slow fire, are filtered with gauze, filter residue enters again Row decocts, and filtrate is mixed, it is 40-50% that low temperature, which is concentrated into water content, is saved backup in 3-4 DEG C；Chinese medicine preparation can strengthen stone The disease-resistant antibacterial ability of spot fish, immunity of organisms is improved, is built up health；

The preparation of liposome：Weigh the sodium dihydrogen phosphate and disodium hydrogen phosphate mixed solution that 0.4-0.5mol/L, pH are 7.5-7.8 80-85 parts, Tea Polyphenols 7.5-8.0 parts are slowly added to, 50-52 DEG C is heated under strong stirring and keeps constant temperature；Weigh yolk ovum Phosphatidase 2 00-250 parts, cholesterol 30-32 parts, VE5-10 parts, are added in 300-350 part absolute ethyl alcohols, are heated to 50-52 DEG C Stirring and dissolving, mixed solution is added in Tea Polyphenols solution after dissolving completely, after keeping temperature strong stirring 30-45 minutes, Rotary evaporation 30-45 minutes remove residual ethanol under 50-55 DEG C of temperature, 0.2-0.3MPa vacuum, are then ultrasonically treated, ultrasonic work( Rate is 200-230W, and frequency is 23-25 KHz, and 3-4 minutes, ultrasound knot are handled with the frequency ultrasound of interval 4-5 seconds ultrasonic 3-4 seconds Tea polyphenol liposomes are placed in 3-4 DEG C of preservation after beam；Tea polyphenol liposomes envelop rate prepared by the method is high, is easy to absorb, and The physics and chemistry processing containing potentially harmful substances such as ether is avoided and preparation method is simple, in preparation process, is especially suitable for industry Change large-scale production；

Extract fish guts oil：Fresh silver carp internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, it is even in refiner Slurry, the homogenate of 200-210 parts fish guts is weighed into container, adds 60-65 part distilled water, stir lower warming-in-water to 30-34 DEG C, PH to 8-9 is adjusted, continues stirring and is warming up to 45-55 DEG C, be incubated 25-35 minutes, adds fish guts weight 8-10% potassium chloride stirring Saltout after dissolving and centrifuge while hot after 20-30 minutes, separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, to enter one Step modification is prepared；This method fish oil yield is high, simple to operate, and waste water, waste residue can be made into green high-efficient fertilizer total integration profit With, be also used as extract nucleic acid raw material it is further high-valued, reached the purpose of waste's reclamation；

Fish oil is modified：Weigh crude fish oil 100-110 parts, vitamin C 0.07-0.08 parts are put in closed container and are well mixed, general Reaction vessel is placed in Ultrasound Instrument, is heated to 70-73 DEG C and is kept, and is continually fed into oxygen 5-8 minutes, is then added desaturase 0.3-0.4 parts, reduced Coenzyme II 0.05-0.06 parts, functional polypeptide 0.02-0.04 parts, it is 400-500W, frequency in power To be stand-by after the 80-90KHz praetersonic effect lower reaction 60-80 seconds；Siphon enzymatic fish oil and with hot water wash 3-4 times, while hot Centrifugation, takes supernatant liquid, and modified fish guts oil is drying to obtain with vacuum drying chamber；The amino acid sequence of functional polypeptide is： SCASRCKSRCRARRCRARRCGIIYFGKRCICKCFRC；Enzymatic reaction can be by the centre position carbon-carbon double bond of saturated fatty acid Hydrogen generation water is seized by oxygen so that chain saturated fatty acids lose hydrogen and are converted into corresponding unrighted acid so that Rich in polyunsaturated fatty acids such as DHA, EPA in fish guts oil；Functional polypeptide can be to the reactant and product of enzymatic reaction Space structure have an impact, increase product in cis unsaturated fatty acid accounting, reduce product in trans unsaturated fat The content of acid, so as to strengthen the nutritive value of unrighted acid, makes it be highly suitable as the fortification composition of grouper；

Compound hardening agent：Weigh Chinese medicine preparation 0.2-0.25 parts, tea polyphenol liposomes 5-10 parts, Modified Fish Oil 30-50 parts, fluorobenzene Buddhist nun examines 0.02-0.03 parts, citric acid 0.2-0.5 parts, compound nucleotide 1.0-2.0 parts, complex enzyme formulation 1.0-2.0 parts, corn Albumen powder 20-25 parts, Gluten 4.0-6.0 parts, peanut meal 5.0-8.0 parts, poly-β-hybroxybutyric acid 0.5-0.8 parts, chlorination courage Alkali 0.3-0.5 parts, taurine 1.0-1.5 parts, ethoxyquin beautiful jade 0.05-0.06 parts, sodium alginate 0.6-0.7 parts, cholesterol Crushed after 0.8-1.0 parts, phagostimulant 2.0-3.0 parts are well mixed, cross 100-120 mesh sieves, outsifting continues to crush until all Sieving, produces grouper nutrition fortifier；Contain abundant traditional Chinese medicine ingredients, phosphatide, unrighted acid, albumen in hardening agent The nutritional ingredients such as matter, complex enzyme, mineral element, coordinate phagostimulant to support chemical conversion point with anti-, be suitable as the fortification of grouper Agent, after being eaten with feed formulation, stress ability, immunocompetence, the disease-resistant antibacterial ability of grouper can be strengthened, promote it to battalion Digesting and assimilating for material is supported, accelerates growing for grouper.

Preferably, compound nucleotide used is adenosine acid disodium and guanosine acid disodium and cytimidine The mixture of disodium 5 '-ribonucleotide and uridine acid disodium and inosinic acid disodium；Nucleotides can be used as protein Kinases strengthens metabolic enzyme activity, the synthesis of inducing hormone or enzyme, promotes the synthesis of juvenile fish vivo protein, so that increase body Weight, promote growth.

Preferably, complex enzyme formulation used be protease and amylase with cellulase and carbohydrase and lipase with The mixture of pectase；Complex enzyme formulation can improve grouper and nutritional ingredient is digested and assimilated, and promote it to grow.

Preferably, phagostimulant used is glycine betaine or neopentanoic acid thetin or glycine or dusty yeast or alanine Or 5- inosinyl phosphate inosines or dried orange peel or brewex's grains or dried silkworm chrysalis meal；Composite feeding promoting agent can strengthen the palatability of feed, so as to promote children Fish feeds.

Compared with prior art, the advantage of the invention is that：1）Chinese medicine preparation in grouper nutrition fortifier can increase The disease-resistant antibacterial ability of strong grouper, improves immunity of organisms, builds up health；2）By being modified to fish guts oil, make fish The polyunsaturated fatty acids such as DHA, EPA are rich in Application of Viscera Oil, the nutrition fortifier of grouper are highly suitable as, when by grouper After feed, Modified Fish Oil can improve the lipid structure in grouper body；3）In hardening agent containing abundant traditional Chinese medicine ingredients, phosphatide, The nutritional ingredients such as unrighted acid, protein, complex enzyme, mineral element, coordinate phagostimulant to support chemical conversion point with anti-, be suitable as The nutrition fortifier of grouper, with feed formulation it is edible after, the stress ability of grouper, immunocompetence, disease-resistant anti-can be strengthened Bacterium ability, promotes it to digest and assimilate nutriment, accelerates growing for grouper.

Embodiment

The present invention program is described further below by embodiment：

Embodiment 1：

The preparation method of grouper nutrition fortifier, comprises the following steps：

1）10 portions of coptiss, 3 parts of fleece-flowers root, 5 portions of golden cypresses and 10 parts of masts are crushed with pulverizer respectively, 80 mesh sieves are crossed, in being made Medicine compound preparation, 10 parts of compound Chinese medicinal preparations are weighed, are wrapped with degreasing cotton yarn, added in 100 parts of water, it is boiled after soaking 1 hour, It is cooked by slow fire 30 minutes, is filtered with gauze, filter residue is decocted again, and filtrate is mixed, and low temperature is concentrated into water content and is 40%, saved backup in 3 DEG C；Chinese medicine preparation can strengthen the disease-resistant antibacterial ability of grouper, improve immunity of organisms, reinforcement Matter；

2）80 parts of the sodium dihydrogen phosphate and disodium hydrogen phosphate mixed solution that 0.4mol/L, pH are 7.5 are weighed, is slowly added to Tea Polyphenols 7.5 parts, 50 DEG C are heated under strong stirring and keeps constant temperature；200 parts of egg yolk lecithin, 30 parts of cholesterol, VE5 parts are weighed, is added Enter into 300 parts of absolute ethyl alcohols, be heated to 50 DEG C of stirring and dissolvings, add mixed solution in Tea Polyphenols solution after dissolving completely, After keeping temperature strong stirring 30 minutes, rotary evaporation removes residual ethanol in 30 minutes under 50 DEG C of temperature, 0.2MPa vacuum, so After be ultrasonically treated, ultrasonic power 200W, frequency 23KHz, handled 3 minutes, surpassed with ultrasonic 3 seconds intervals frequency ultrasound of 4 seconds Tea polyphenol liposomes are placed in 3 DEG C of preservations by sound after terminating；

3）Take 200 parts of fresh silver carp internal organ homogenate to add 60 parts of distilled water into container, stir lower warming-in-water to 30 DEG C, adjust PH to 8 is saved, continues stirring and is warming up to 45 DEG C, be incubated 25 minutes, is taken advantage of after being saltoutd 20 minutes after 16 parts of potassium chloride stirring and dissolvings of addition Heat centrifugation, separates to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, is prepared for further modification；

4）Weigh 100 parts of crude fish oil, 0.07 part of vitamin C be put in closed container be well mixed, reaction vessel is placed in ultrasound In instrument, it is heated to 70 DEG C and keeps, be continually fed into oxygen 5 minutes, then adds 0.3 part of desaturase, reduced Coenzyme II 0.05 part, 0.02 part of functional polypeptide, treated after being reacted 60 seconds under the praetersonic effect that power is 400W, frequency is 80KHz With；Siphon enzymatic fish oil and with hot water wash 3 times, centrifuges, takes supernatant liquid, fish guts are drying to obtain with vacuum drying chamber while hot Oil；The hydrogen of the centre position carbon-carbon double bond of saturated fatty acid can be seized generation water by enzymatic reaction by oxygen, so that long-chain saturation Aliphatic acid loses hydrogen and is converted into corresponding unrighted acid, so that rich in how unsaturated fat such as DHA, EPA in fish guts oil Fat acid；

5）Weigh 0.2 part of Chinese medicine preparation, 5 parts of tea polyphenol liposomes, 30 parts of Modified Fish Oil, 0.02 part of Florfenicol, citric acid 0.2 Part, 1.0 parts of compound nucleotide, 1.0 parts of complex enzyme formulation, 20 parts of corn protein powder, 4.0 parts of Gluten, 5.0 parts of peanut meal, gather 0.5 part of beta-hydroxy-butanoic acid ester, 0.3 part of Choline Chloride, 1.0 parts of taurine, 0.05 part of ethoxyquin beautiful jade, 0.6 part of sodium alginate, courage Crushed after 0.8 part of sterol, 1.0 parts of glycine betaine, 1.0 parts of neopentanoic acid thetin are well mixed, cross 100 mesh sieves, outsifting continues Crush until all sievings, produce grouper nutrition fortifier；Contain abundant traditional Chinese medicine ingredients, phosphatide, unsaturation in hardening agent The nutritional ingredients such as aliphatic acid, protein, complex enzyme, mineral element, coordinate phagostimulant to support chemical conversion point with anti-, be suitable as grouper Nutrition fortifier, with feed formulation it is edible after, stress ability, immunocompetence, the disease-resistant antibacterial ability of grouper can be strengthened, Promote it to digest and assimilate nutriment, accelerate growing for grouper.

Embodiment 2：

1）The preparation of Chinese medicine preparation：15 portions of coptiss, 5 parts of fleece-flowers root, 8 portions of golden cypresses and 15 parts of masts are crushed with pulverizer respectively, 100 mesh sieves are crossed, compound Chinese medicinal preparation is made, 12 parts of compound Chinese medicinal preparations is weighed, is wrapped with degreasing cotton yarn, are added in 120 parts of water, It is boiled after soaking 2 hours, it is cooked by slow fire 40 minutes, is filtered with gauze, filter residue is decocted again, and filtrate is mixed, low temperature It is 50% to be concentrated into water content, is saved backup in 4 DEG C；Chinese medicine preparation can strengthen the disease-resistant antibacterial ability of grouper, improve body Immunity, build up health；

2）The preparation of liposome：85 parts of the sodium dihydrogen phosphate and disodium hydrogen phosphate mixed solution that 0.5mol/L, pH are 7.8 are weighed, 8.0 parts of Tea Polyphenols is slowly added to, 52 DEG C are heated under strong stirring and keeps constant temperature；Weigh 250 parts of egg yolk lecithin, cholesterol 32 parts, VE10 parts, it is added in 350 parts of absolute ethyl alcohols, is heated to 52 DEG C of stirring and dissolvings, adds mixed solution after dissolving completely In Tea Polyphenols solution, keeping temperature strong stirring is after 45 minutes, and rotary evaporation removes for 45 minutes under 55 DEG C of temperature, 0.3MPa vacuum Residual ethanol is gone, is then ultrasonically treated, ultrasonic power 230W, frequency is 25 KHz, is surpassed with ultrasonic 4 seconds intervals frequency of 5 seconds Tea polyphenol liposomes are placed in 4 DEG C of preservations by sonication 4 minutes, ultrasound after terminating；Tea polyphenol liposomes encapsulating prepared by the method Rate is high, is easy to absorb, and preparation method is simple；

3）Extract fish guts oil：Fresh silver carp internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, in refiner Homogenate, 210 parts of fish guts homogenate are weighed into container, add 65 parts of distilled water, stir lower warming-in-water to 34 DEG C, regulation pH is arrived 9, continue stirring and be warming up to 55 DEG C, be incubated 35 minutes, centrifuged while hot after being saltoutd 30 minutes after 21 parts of potassium chloride stirring and dissolvings of addition, Separate to obtain crude fish oil；This method fish oil yield is high, simple to operate, and waste water, waste residue can be made into green high-efficient fertilizer total integration profit With, be also used as extract nucleic acid raw material it is further high-valued, reached the purpose of waste's reclamation；

4）Fish oil is modified：Weigh 110 parts of crude fish oil, 0.08 part of vitamin C be put in closed container be well mixed, by reaction vessel It is placed in Ultrasound Instrument, is heated to 73 DEG C and keeps, be continually fed into oxygen 8 minutes, then adds 0.4 part of desaturase, reduced form 0.06 part of codehydrogenase Ⅱ, 0.04 part of functional polypeptide, react 80 under the praetersonic effect that power is 500W, frequency is 90KHz It is stand-by after second；Siphon enzymatic fish oil and with hot water wash 4 times, centrifuges, takes supernatant liquid, fish is drying to obtain with vacuum drying chamber while hot Application of Viscera Oil；The amino acid sequence of functional polypeptide is：SCASRCKSRCRARRCRARRCGIIYFGKRCICKCFRC；Enzymatic reaction The hydrogen of the centre position carbon-carbon double bond of saturated fatty acid can be seized generation water by oxygen, so that chain saturated fatty acids lose hydrogen Corresponding unrighted acid is converted into, so that rich in polyunsaturated fatty acids such as DHA, EPA in fish guts oil；

5）Compound hardening agent：Weigh 0.25 part of Chinese medicine preparation, 10 parts of tea polyphenol liposomes, 50 parts of Modified Fish Oil, Florfenicol 0.03 part, 0.5 part of citric acid, 2.0 parts of compound nucleotide, 2.0 parts of complex enzyme formulation, 25 parts of corn protein powder, Gluten 6.0 Part, 8.0 parts of peanut meal, 0.8 part of poly-β-hybroxybutyric acid, 0.5 part of Choline Chloride, 1.5 parts of taurine, ethoxyquin beautiful jade 0.06 Part, 0.7 part of sodium alginate, 1.0 parts of cholesterol, 1.0 parts of alanine, 1.0 parts of 5- inosinyl phosphate inosines, 1.0 parts of dried silkworm chrysalis meal are well mixed After crush, cross 120 mesh sieves, outsifting continue crush until all sieving, produce grouper nutrition fortifier.

Embodiment 3：

The preparation method of grouper nutrition fortifier, including：The preparation of Chinese medicine preparation, the preparation of liposome, extraction fish guts oil, Fish oil is modified, compounding hardening agent, specifically includes following steps：

The preparation of Chinese medicine preparation：12 portions of coptiss, 4 parts of fleece-flowers root, 6 portions of golden cypresses and 14 parts of masts are crushed with pulverizer respectively, mistake 80 mesh sieves, are made compound Chinese medicinal preparation, weigh 12 parts of compound Chinese medicinal preparations, are wrapped with degreasing cotton yarn, add in 120 parts of water, leaching Bubble is boiled after 2 hours, is cooked by slow fire 40 minutes, is filtered with gauze, filter residue is decocted again, and filtrate is mixed, and low temperature is dense It is 45% to be reduced to water content, is saved backup in 4 DEG C；Chinese medicine preparation can strengthen the disease-resistant antibacterial ability of grouper, improve body and exempt from Epidemic disease power, builds up health；

The preparation of liposome：80 parts of the sodium dihydrogen phosphate and disodium hydrogen phosphate mixed solution that 0.4mol/L, pH are 7.6 are weighed, is delayed It is slow to add 8.0 parts of Tea Polyphenols, it is heated to 52 DEG C under strong stirring and keeps constant temperature；Weigh 240 parts of egg yolk lecithin, cholesterol 32 Part, VE8 parts, are added in 350 parts of absolute ethyl alcohols, are heated to 50 DEG C of stirring and dissolvings, mixed solution is added into tea after dissolving completely In more phenol solutions, keeping temperature strong stirring is after 35 minutes, and rotary evaporation removes for 45 minutes under 52 DEG C of temperature, 0.2MPa vacuum Residual ethanol, then it is ultrasonically treated, ultrasonic power 200W, frequency 23KHz, at the frequency ultrasound of 5 seconds of ultrasonic 4 seconds intervals Tea polyphenol liposomes are placed in 4 DEG C of preservations by reason 3 minutes, ultrasound after terminating；Tea polyphenol liposomes envelop rate prepared by the method is high, It is easy to absorb, and preparation method is simple, and the physics and chemistry processing containing potentially harmful substances such as ether is avoided in preparation process, it is non- Often it is adapted to industrialization large-scale production；

Extract fish guts oil：Fresh silver carp internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, it is even in refiner Slurries, 200 parts of fish guts homogenate are weighed into container, add 65 parts of distilled water, stir lower warming-in-water to 32 DEG C, adjust pH to 8, Continue stirring and be warming up to 55 DEG C, be incubated 35 minutes, centrifuged while hot after being saltoutd 20 minutes after 16 parts of potassium chloride stirring and dissolvings of addition, point From crude fish oil；Potassium method extracts fish guts oil to remove impurity, is prepared for further modification；This method fish oil yield is high, behaviour Make simply, waste water, waste residue can be made into the utilization of green high-efficient fertilizer total integration, and the raw material for being also used as extracting nucleic acid is further It is high-valued, reach the purpose of waste's reclamation；

Fish oil is modified：Weigh 110 parts of crude fish oil, 0.08 part of vitamin C be put in closed container be well mixed, reaction vessel is put In Ultrasound Instrument, it is heated to 73 DEG C and keeps, be continually fed into oxygen 8 minutes, it is auxiliary then adds 0.4 part of desaturase, reduced form II 0.05 parts of enzyme, 0.02 part of functional polypeptide, reacted 65 seconds under the praetersonic effect that power is 400W, frequency is 90KHz It is stand-by afterwards；Siphon enzymatic fish oil and with hot water wash 4 times, centrifuges, takes supernatant liquid, be drying to obtain with vacuum drying chamber in fish while hot Dirty oil；The amino acid sequence of functional polypeptide is：SCASRCKSRCRARRCRARRCGIIYFGKRCICKCFRC；Enzymatic reaction energy The hydrogen of the centre position carbon-carbon double bond of saturated fatty acid is seized into generation water by oxygen, so that chain saturated fatty acids lose hydrogen and turned Corresponding unrighted acid is turned to, so that rich in polyunsaturated fatty acids such as DHA, EPA in fish guts oil；Functional polypeptide The reactant of enzymatic reaction and the space structure of product can be had an impact, cis unsaturated fatty acid accounts in increase product Than reducing the content of trans unrighted acid in product, so as to strengthen the nutritive value of unrighted acid, making it very suitable Cooperate the fortification composition for grouper；

Compound hardening agent：Weigh 0.2 part of Chinese medicine preparation, 8 parts of tea polyphenol liposomes, 32 parts of Modified Fish Oil, 0.02 part of Florfenicol, 0.4 part of citric acid, 2.0 parts of compound nucleotide, 1.5 parts of complex enzyme formulation, 24 parts of corn protein powder, 6.0 parts of Gluten, peanut meal 6.5 parts, 0.6 part of poly-β-hybroxybutyric acid, 0.3 part of Choline Chloride, 1.2 parts of taurine, 0.05 part of ethoxyquin beautiful jade, sodium alginate 0.6 part, 0.8 part of cholesterol, 0.5 part of glycine betaine, 0.5 part of alanine, 1.0 parts of dried orange peel it is well mixed after crush, cross 120 mesh sieves, Outsifting continues to crush until all sievings, produce grouper nutrition fortifier；Contain abundant traditional Chinese medicine ingredients, phosphorus in hardening agent The nutritional ingredients such as fat, unrighted acid, protein, complex enzyme, mineral element, coordinate phagostimulant to support chemical conversion point with anti-, be adapted to As the nutrition fortifier of grouper, with feed formulation it is edible after, the stress ability of grouper, immunocompetence, anti-can be strengthened Sick antibacterial ability, promotes it to digest and assimilate nutriment, accelerates growing for grouper.

Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.

Technical scheme is described in detail embodiment described above, it should be understood that it is described above only For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should be included in the scope of the protection.

Sequence table

<110>Animal feed technological development Co., Ltd is full of by Pujiang County association

<120>The preparation method of grouper nutrition fortifier

<130> 3

<160> 1

<170> SIPOSequenceListing 1.0

<210> 2

<211> 36

<212> PRT

<213>Artificial synthesized (Mytilus coruscus)

<400> 2

Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Arg

1 5 10 15

Ala Arg Arg Cys Gly Ile Ile Tyr Phe Gly Lys Arg Cys Ile Cys Lys

20 25 30

Cys Phe Arg Cys

35

Claims

1. the preparation method of grouper nutrition fortifier, including：The preparation of Chinese medicine preparation, the preparation of liposome, extraction fish guts Oil, fish oil are modified, compounding hardening agent, it is characterised in that：The step of described compounding hardening agent is：It is more to weigh Chinese medicine preparation, tea Crushed after phenol liposome, Modified Fish Oil, compound nucleotide, complex enzyme formulation, phagostimulant are well mixed, cross 100-120 mesh sieves, sieve Go out thing to continue to crush until all sievings, produce grouper nutrition fortifier.

2. the preparation method of grouper nutrition fortifier according to claim 1, it is characterised in that：Described Chinese medicine preparation Preparation steps be：10-15 parts coptis, 3-5 parts fleece-flower root, 5-8 parts golden cypress and 10-15 parts mast are used into pulverizer powder respectively It is broken, 80-100 mesh sieves are crossed, compound Chinese medicinal preparation is made, weighs 10-12 part compound Chinese medicinal preparations, is wrapped with degreasing cotton yarn, are added It is boiled after immersion 1-2 hours in 100-120 part water, 30-40 minutes are cooked by slow fire, are filtered with gauze, filter residue is decocted again Boil, filtrate is mixed, it is 40-50% that low temperature, which is concentrated into water content, is saved backup in 3-4 DEG C.

3. the preparation method of grouper nutrition fortifier according to claim 1, it is characterised in that：Described liposome Preparation process be：Weigh the sodium dihydrogen phosphate and disodium hydrogen phosphate mixed solution 80- that 0.4-0.5mol/L, pH are 7.5-7.8 85 parts, Tea Polyphenols 7.5-8.0 parts are slowly added to, 50-52 DEG C is heated under strong stirring and keeps constant temperature；Weigh egg yolk lecithin 200-250 parts, cholesterol 30-32 parts, VE5-10 parts, are added in 300-350 part absolute ethyl alcohols, are heated to 50-52 DEG C of stirring Dissolving, mixed solution is added in Tea Polyphenols solution after dissolving completely, after keeping temperature strong stirring 30-45 minutes, in 50-55 Rotary evaporation 30-45 minutes remove residual ethanol under DEG C temperature, 0.2-0.3MPa vacuum, are then ultrasonically treated, ultrasonic power is 200-230W, frequency are 23-25 KHz, 3-4 minutes are handled with the frequency ultrasound of interval 4-5 seconds ultrasonic 3-4 seconds, after ultrasound terminates Tea polyphenol liposomes are placed in 3-4 DEG C of preservation.

4. the preparation method of grouper nutrition fortifier according to claim 1, it is characterised in that：In described extraction fish Dirty oily step is：Fresh silver carp internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, be homogenized, weigh in refiner 200-210 parts fish guts are homogenized into container, add 60-65 part distilled water, are stirred lower warming-in-water to 30-34 DEG C, are adjusted pH To 8-9, continue stirring and be warming up to 45-55 DEG C, be incubated 25-35 minutes, after adding fish guts weight 8-10% potassium chloride stirring and dissolving Saltout and centrifuge while hot after 20-30 minutes, separate to obtain crude fish oil.

5. the preparation method of grouper nutrition fortifier according to claim 1, it is characterised in that：Described fish oil is modified Step is：Weigh crude fish oil 100-110 parts, vitamin C 0.07-0.08 parts be put in closed container be well mixed, will reaction hold Device is placed in Ultrasound Instrument, is heated to 70-73 DEG C and is kept, and is continually fed into oxygen 5-8 minutes, then adds desaturase 0.3- 0.4 part, reduced Coenzyme II 0.05-0.06 parts, functional polypeptide 0.02-0.04 parts, power be 400-500W, frequency be It is stand-by after the 80-90KHz praetersonic effect lower reaction 60-80 seconds；Siphon enzymatic fish oil and with hot water wash 3-4 times, while hot from The heart, supernatant liquid is taken, modified fish guts oil is drying to obtain with vacuum drying chamber.

6. the preparation method of grouper nutrition fortifier according to claim 5, it is characterised in that：Described fish oil is modified The amino acid sequence of functional polypeptide in step is：SCASRCKSRCRARRCRARRCGIIYFGKRCICKCFRC.

7. the preparation method of grouper nutrition fortifier according to claim 1, it is characterised in that：Described compounding is strengthened Agent step is：Weigh Chinese medicine preparation 0.2-0.25 parts, tea polyphenol liposomes 5-10 parts, Modified Fish Oil 30-50 parts, Florfenicol 0.02-0.03 parts, citric acid 0.2-0.5 parts, compound nucleotide 1.0-2.0 parts, complex enzyme formulation 1.0-2.0 parts, zein Powder 20-25 parts, Gluten 4.0-6.0 parts, peanut meal 5.0-8.0 parts, poly-β-hybroxybutyric acid 0.5-0.8 parts, Choline Chloride 0.3-0.5 parts, taurine 1.0-1.5 parts, ethoxyquin beautiful jade 0.05-0.06 parts, sodium alginate 0.6-0.7 parts, cholesterol 0.8- 1.0 being crushed after part, phagostimulant 2.0-3.0 parts are well mixed, 100-120 mesh sieves are crossed, outsifting continues to crush up to all sievings, Produce grouper nutrition fortifier.

8. the preparation method of grouper nutrition fortifier according to claim 7, it is characterised in that：Described compounding is strengthened Compound nucleotide in agent step for adenosine acid disodium and guanosine acid disodium and cytidine acid disodium with The mixture of uridine acid disodium and inosinic acid disodium, complex enzyme formulation are protease and amylase and fiber Plain enzyme and carbohydrase and lipase and the mixture of pectase, phagostimulant be glycine betaine or neopentanoic acid thetin or glycine or Dusty yeast or alanine or 5- inosinyl phosphate inosines or dried orange peel or brewex's grains or dried silkworm chrysalis meal.