CN107595896A - The preparation method of one main laminaria polyphenol and its application - Google Patents
The preparation method of one main laminaria polyphenol and its application Download PDFInfo
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- CN107595896A CN107595896A CN201710823313.0A CN201710823313A CN107595896A CN 107595896 A CN107595896 A CN 107595896A CN 201710823313 A CN201710823313 A CN 201710823313A CN 107595896 A CN107595896 A CN 107595896A
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Abstract
The invention belongs to kelp processing preparation field, the method and its application of sea-tangle polyphenol are extracted in a more particularly to main laminaria.Its technical scheme is：The preparation method of one main laminaria polyphenol, its feature comprise the following steps：Clean dried and crushed, microwave, enzymolysis pre-treatment, water-bath extraction, vacuum rotary steam.Obtained sea-tangle polyphenol is applied to treatment diabetes.Microwave of the present invention and the compound extraction of enzymolysis are pre-treatment means, sea-tangle polyphenol are made using solvent extraction method, operating procedure is simple, recovery rate is high, is up to 2.78%；Obtained sea-tangle polyphenol is applied to treatment diabetes, can significantly reduce blood glucose value.
Description
Technical field
The invention belongs to kelp processing preparation field, the method for sea-tangle polyphenol is extracted in a more particularly to main laminaria and its is answered
With.
Background technology
Marine alga is lived under special ecological environment, generally contains bioactive substance such as phenols, Yin with specific function
The compounds such as diindyl class, terpene, it is the important sources of marine natural medicine.Seeweed polyphenol (algaepolyphenols, AP) be from
The polyphenol compound general name extracted in marine alga, turn into the research heat in phenols field because of unique physiological function that it has
Point.Then content is larger in brown alga for seeweed polyphenol class compound, and species is more.Phaeophyta there are about 250 category, more than 1500 kinds, be
An advanced big monoid in marine alga.The common brown alga of China has sargassum kjellmanianum Yendo (Sar-gassumkjellmanianum), sargassum thunbergii
(Sargas-sumthunbergii), sea-tangle (Laminariajaponi-ca), undaria pinnitafida (Undariapinnatifida).
This special environment of marine alga long term growth Yu Haiyang, many active components caused by it are that land biology institute can not
Analogy.China's algae culturing industry occupy world lead level, marine algae resource very abundant, is the high-valued of China's marine algae resource
Using providing abundant raw material.Wherein, sea-tangle polyphenol, it is a kind of important brown algae polyphenols compound, because sea-tangle polyphenol has
There is the bioactivity such as antitumor, antiviral, therefore get more and more people's extensive concerning.
The extraction process of plant polyphenol there has been suitable research at present, but focus mostly in terrestrial plant.Wherein solvent soaks
Carry, precipitation by metallic ion is research and the more extraction method of report, have also appeared some new methods such as resin in recent years and inhaled
Attached method, biological enzymolysis extraction, membrane technology extraction, supercritical fluid extraction, ultrasonic-leaching method and microwave digestion method etc., the above
Method respectively has its strong point.The change of Content of Phlorotannins is relevant with several factors, and extraction process must be simply efficient.
To realize the deep development of sea-tangle and comprehensive utilization, the extraction process of sea-tangle polyphenol is studied, is current urgent need to resolve
Technical problem.
The content of the invention
In order to solve it is above-mentioned the problems of in the prior art, it is an object of the invention to provide a main laminaria polyphenol
Preparation method and its application.
Its technical scheme is：The preparation method of one main laminaria polyphenol, its feature comprise the following steps：
A. dried and crushed is cleaned：Fresh Laminaria Japonica is cleaned with running water and crushed after naturally dry with pulverizer；
B. microwave：The ethanol of sea-tangle and 50%~90% will be crushed is 1 in mass ratio:2~1:10 mixing, enter
Row microwave；
C. pre-treatment is digested：Pectase will be added in mixture after microwave treatment, regulation pH value is 5.0~6.5, is placed in
In 50 DEG C~80 DEG C of thermostat water bath, taken out after digesting 1h~5h；
D. water-bath extracts：It is then placed in 50 DEG C~80 DEG C shaking baths, rotating speed 100r/min, lucifuge extraction 1h~6h；
E. vacuum rotary steam：Take supernatant to carry out vacuum rotary steam and remove ethanol, obtain sea-tangle polyphenol.
Further, the microwave power of the microwave described in step B is 200W~1500W, the time be 30s~
150s, temperature are 50 DEG C~80 DEG C；
Further, the microwave power of the microwave described in step B is 1000W, and time 70s, temperature is 70 DEG C；
Concentration of alcohol is 80%, mass ratio 1:7；
Further, the enzymolysis pre-treatment pectase addition 20000U/kg described in step C, regulation pH value are 6.0, water
Bath temperature is 60 DEG C, enzymolysis time 2h；
Further, the bath temperature described in step D is 60 DEG C, extraction time 4h；
Further, 3 progress of water-bath extraction point described in step D.
Further, applied to treatment diabetes.
Microwave of the present invention and the compound extraction of enzymolysis are pre-treatment means, and sea-tangle polyphenol is made using solvent extraction method, operates
Step is simple, and the recovery rate of sea-tangle polyphenol is high, and up to 2.78%；Obtained sea-tangle polyphenol is applied to treatment diabetes, can show
Writing reduces blood glucose value.
Brief description of the drawings
Embodiment
Technical scheme is explained with reference to accompanying drawing as follows.
Example 1：The preparation method of one main laminaria polyphenol, its feature comprise the following steps：
A. dried and crushed is cleaned：Fresh Laminaria Japonica is cleaned with running water and crushed after naturally dry with pulverizer；
B. microwave：Sea-tangle 1g will be crushed to mix with 80% ethanol 50mL, carry out microwave, microwave power
For 1000W, time 70s, temperature is 70 DEG C；
C. pre-treatment is digested：Mixture after microwave treatment is added into pectase by 20000U/kg, regulation pH value is 6.0,
It is placed in 60 DEG C of thermostat water bath, is taken out after digesting 2h；
D. water-bath extracts：It is then placed in 60 DEG C of shaking baths, rotating speed 100r/min, lucifuge extraction 4h, point 3 progress；
E. vacuum rotary steam：Take supernatant to carry out vacuum rotary steam and remove ethanol, obtain sea-tangle polyphenol recovery rate and reach 2.78%.
Example 2：The preparation method of one main laminaria polyphenol, its feature comprise the following steps：
A. dried and crushed is cleaned：Fresh Laminaria Japonica is cleaned with running water and crushed after naturally dry with pulverizer；
B. microwave：Sea-tangle 1g will be crushed to mix with 80% ethanol 50mL, carry out microwave, microwave power
For 1000W, time 70s, temperature is 70 DEG C；
C. pre-treatment is digested：Mixture after microwave treatment is added into pectase by 20000U/kg, regulation pH value is 6.0,
It is placed in 60 DEG C of thermostat water bath, is taken out after digesting 2h；
D. water-bath extracts：It is then placed in 60 DEG C of shaking baths, rotating speed 100r/min, lucifuge extraction 6h；
E. vacuum rotary steam：Take supernatant to carry out vacuum rotary steam and remove ethanol, obtain sea-tangle polyphenol recovery rate and reach 2.39%.
Example 3：The preparation method of one main laminaria polyphenol, its feature comprise the following steps：
A. dried and crushed is cleaned：Fresh Laminaria Japonica is cleaned with running water and crushed after naturally dry with pulverizer；
B. microwave：Sea-tangle 1g will be crushed to mix with 80% ethanol 80mL, carry out microwave, microwave power
For 800W, time 100s, temperature is 80 DEG C；
C. pre-treatment is digested：Mixture after microwave treatment is added into pectase by 20000U/kg, regulation pH value is 6.0,
It is placed in 60 DEG C of thermostat water bath, is taken out after digesting 3h；
D. water-bath extracts：It is then placed in 60 DEG C of shaking baths, rotating speed 100r/min, lucifuge extraction 4h；
E. vacuum rotary steam：Take supernatant to carry out vacuum rotary steam and remove ethanol, obtain sea-tangle polyphenol recovery rate and reach 2.09%.
Example 4：The application of sea-tangle polyphenol as made from embodiment 1, applied to treatment diabetic, blood glucose significantly drops
It is low.Diabetic mice is tested, after to the dosage group of diabetic mice gavage (0.1g/kg.d) 14 days, its blood glucose value shows
Work property reduces 31.98%；For high dose group (0.2g/kg.d) after 14 days, its blood glucose value conspicuousness reduces 50.24% (P ＜
0.01)。
Blood sugar reducing function of the sea-tangle polyphenol of table 1 to blood glucose in diabetic rats value
Claims (7)
1. the preparation method of a main laminaria polyphenol, its feature comprise the following steps：
A. dried and crushed is cleaned：Fresh Laminaria Japonica is cleaned with running water and crushed after naturally dry with pulverizer；
B. microwave：The ethanol of sea-tangle and 50%~90% will be crushed is 1 in mass ratio:2~1:10 mixing, are carried out micro-
Wavefront processing；
C. pre-treatment is digested：Pectase will be added in mixture after microwave treatment, regulation pH value is 5.0~6.5, is placed in 50 DEG C
In~80 DEG C of thermostat water bath, taken out after digesting 1h~5h；
D. water-bath extracts：It is then placed in 50 DEG C~80 DEG C shaking baths, rotating speed 100r/min, lucifuge extraction 1h~6h；
E. vacuum rotary steam：Take supernatant to carry out vacuum rotary steam and remove ethanol, obtain sea-tangle polyphenol.
2. the preparation method of main laminaria polyphenol as claimed in claim 1, it is characterized in that：Microwave described in step B
Microwave power be 200W~1500W, the time is 30s~150s, and temperature is 50 DEG C~80 DEG C.
3. the preparation method of main laminaria polyphenol as claimed in claim 1, it is characterized in that：Microwave described in step B
Microwave power be 1000W, time 70s, temperature is 70 DEG C；Concentration of alcohol is 80%, mass ratio 1:7.
4. the preparation method of the main laminaria polyphenol as described in claim 1-3 is one of any, it is characterized in that：Described in step C
Pre-treatment pectase addition 20000U/kg is digested, regulation pH value is 6.0, and bath temperature is 60 DEG C, enzymolysis time 2h.
5. the preparation method of the main laminaria polyphenol as described in claim 1-4 is one of any, it is characterized in that：Described in step D
Bath temperature is 60 DEG C, extraction time 4h.
6. the preparation method of the main laminaria polyphenol as described in claim 1-5 is one of any, it is characterized in that：Water described in step D
3 progress of bath extraction point.
7. the application of sea-tangle polyphenol as made from claim 1-5 is one of any, it is characterized in that applied to treatment diabetes.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111000875A (en) * | 2019-11-18 | 2020-04-14 | 青岛科技大学 | Extraction method of kelp polyphenol |
| CN113662112A (en) * | 2021-08-27 | 2021-11-19 | 大连工业大学 | Compound fruit and vegetable juice with eye protection function and preparation method thereof |
| CN115380918A (en) * | 2022-08-29 | 2022-11-25 | 福州大学 | Response surface method optimized extraction method of kelp polyphenol |
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| KR101201987B1 (en) * | 2009-03-05 | 2012-11-15 | 주식회사 보양 | Method of manufacturing functional sea tangle for boiled rice with sea tangle |
| CN106619744A (en) * | 2017-02-08 | 2017-05-10 | 中英阿诺康（宁夏）生物科技有限公司 | Extraction technique and preparation method of algal polyphenols |
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2017
- 2017-09-13 CN CN201710823313.0A patent/CN107595896A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101201987B1 (en) * | 2009-03-05 | 2012-11-15 | 주식회사 보양 | Method of manufacturing functional sea tangle for boiled rice with sea tangle |
| CN106619744A (en) * | 2017-02-08 | 2017-05-10 | 中英阿诺康（宁夏）生物科技有限公司 | Extraction technique and preparation method of algal polyphenols |
Non-Patent Citations (1)
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| 张振国: "褐藻多酚衍生物潜在的药物应用", 《辽宁化工》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111000875A (en) * | 2019-11-18 | 2020-04-14 | 青岛科技大学 | Extraction method of kelp polyphenol |
| CN113662112A (en) * | 2021-08-27 | 2021-11-19 | 大连工业大学 | Compound fruit and vegetable juice with eye protection function and preparation method thereof |
| CN115380918A (en) * | 2022-08-29 | 2022-11-25 | 福州大学 | Response surface method optimized extraction method of kelp polyphenol |
| CN115380918B (en) * | 2022-08-29 | 2024-01-26 | 福州大学 | Method for optimized extraction of kelp polyphenol by response surface method |
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