CN107593961A

CN107593961A - A kind of gingkgo health care fermented tea and its preparation method and application

Info

Publication number: CN107593961A
Application number: CN201610544325.5A
Authority: CN
Inventors: 黄海波; 陈岑; 张小贵; 乔洪翔; 任红; 蒋亚良; 田飞宇; 于国栋; 王如伟; 陈君; 李�杰; 沈晓蓉
Original assignee: Yunnan Hikon Biotechnology Co Ltd; Yunnan Kangenbei Plant Research Institute Co Ltd; ZHEJIANG CONBA PHARMACEUTICAL CO Ltd; Yunnan Grand Early Food Co Ltd
Current assignee: Yunnan Hikon Biotechnology Co Ltd; Yunnan Kangenbei Plant Research Institute Co Ltd; ZHEJIANG CONBA PHARMACEUTICAL CO Ltd; Yunnan Grand Early Food Co Ltd
Priority date: 2016-07-12
Filing date: 2016-07-12
Publication date: 2018-01-19
Anticipated expiration: 2036-07-12
Also published as: CN107593961B

Abstract

The invention discloses a kind of gingkgo health care fermented tea and its preparation method and application.P. kingianum, bitter buckwheat, jujube are extracted with hydrous ethanol respectively, after extracting concentrated liquor dissolved in purified water, are well mixed to be placed in fermenting house with large leaf sun withering tea, ginkgo leaf and STEVIA REBAUDIANA and are fermented 20~50 days, produce gingkgo health care fermented tea.Present invention process is simple, and fermentation time is short, is advantageous to industrial production.STEVIA REBAUDIANA, bitter buckwheat and the jujube of addition can improve the bitter taste of ginkgo leaf, abundant mouthfeel, make tea flavour mellow, and by optimizing the central temperature of fermented tea heap, the content of ginkgoic acid effectively is controlled, and remains Ginkgolide Component to greatest extent, while Product Safety is improved, health-care efficacy is also increased, is had a good application prospect.

Description

A kind of gingkgo health care fermented tea and its preparation method and application

Technical field

The invention belongs to tea product zymolysis process technology field, and in particular to a kind of gingkgo health care fermented tea and preparation side Method.

Background technology

Ginkgo is Ginkgoaceae ginkgo platymiscium, is one of Relict Plant most ancient in the world, have " plant living fossil it Claim ".Ginkgo leaf is the main medicinal part of ginkgo, and its chemical composition includes flavonoids, terpene lactone, ginkgoic acid, organic acid, poly- Pentenol etc..Pharmacological research shows that ginkgo leaf, which has, removes free radical and peroxide, reduces blood fat, improves cerebral ischemia, suppresses Formation of platelet aggregation and thrombus and other effects, it is usually used in preventing, treats cardiovascular and cerebrovascular disease.Due to its unique medicinal, guarantor Strong value, develops the health product based on ginkgo leaf and gradually rises.Ginkgo leave tea are using ginkgo leaf as raw material, arrange in pairs or groups or do not arrange in pairs or groups A kind of Novel health beverage of tealeaves or other Chinese medicines, for prevention or auxiliary treatment atherosclerosis, high fat of blood, high blood The diseases such as pressure, hyperglycaemia.

Pu'er tea is the distinctive well-known tea in Yunnan,《Chinese tea passes through》Described in its manufacture craft " kneaded and dried with fixing Large leaf solar dried green tea is tea material, is made through pile-fermentation ".It is warm-natured after Pu'er tea is fermented, have slake thirst and help produce saliva, clear sharp head Mesh, promoting digestion greasy, removing toxic substances are sobered up and other effects.Modern pharmacology research shows that Pu'er tea has reducing blood lipid, anti-atherogenic hard Change, prevent the health-care efficacy such as coronary heart disease, antifatigue；Its chemical composition and green tea, black tea etc. differ widely, and research shows, is sending out During ferment, chemical change of the polyphenol components through the complexity such as bioconversion, oxidative condensation based on flavonoids, Tea Polyphenols, Activity higher theaflavin and thearubigin are formed, this is probably the material base of the bioactivity such as Pu'er tea hypoglycemic；Meanwhile These compositions can also make the soup look uniqueness of millet paste, flavour mellow.In addition, soluble polysaccharide, lipid-like substances, protein and life The conversion of thing alkaloids, the fragrance for improving tealeaves is can aid in, increase the fresh refreshing degree of millet paste, form the distinctive taste of Pu'er tea Taste.

At present, commercially available ginkgo leave tea and few, it is difficult to popularize, its reason is probably：First, ginkgo containing toxic component in ginkgo leave tea Acid, research show that ginkgoic acid has sensitization, cytotoxicity and immunotoxicity etc., it is necessary to using certain technical controlling ginkgoic acid Content, potential safety hazard can be produced by otherwise drinking for a long time.2nd, ginkgo leave tea bitterness is highly seasoned, and mouthfeel is bad, and entrance returns hardship；In ginkgo leaf It is both the active material in ginkgo leaf rich in flavonoids and lactone compound, the main reason for being ginkgo leave tea bitter taste again. 3rd, ginkgolides is fat-soluble compound, and directly limited with brewed in hot water active ingredient dissolution, its practical efficiency is relatively It is low.Therefore ginkgo acid content how is reduced, while increase mouthfeel, improve the focus that the utilization rate of active ingredient is studied into ginkgo leave tea And difficult point.

At present, have some ginkgo tea preparation process for being directed to above section problem.The A of Publication No. CN 102835712 《A kind of ginkgo leaf takes off bitter method》After ginkgo biloba p.e is mixed with Fructus Monordicae extract, heating, after testing after de- hardship Through without GINKGO BILOBA EXTRACT and amarolide, thus it is good in taste, but ginkgo acid content is not controlled.The A of publication number CN 101628018 《A kind of preparation method of enriched-flavone low-toxicity gingko leaves》, by ginkgo leaf rinse airing knead after under anoxic conditions itself Fermentation, so as to obtain toxic component reduction, the increased ginkgo leaf of flavones content, but mouthfeel is not good enough.Publication No. CN 104286247《A kind of preparation method of detoxification ginkgoic acid》Using inoculation coronoid process dissipate capsule bacterium as main fermentation virus-free strain, system Standby leavening, ferments for ginkgo leaf, can effectively reduce ginkgo acid substance, removes ginkgo leave tea bitter taste, but separates identification strain, It is complex to prepare leavening, cost is higher.As can be seen here, fermentation process has attenuation, but traditional zymotic to ginkgo leaf Technique, fermentation condition is not controlled, is vulnerable to the harmful effect of external environment, vulnerable to pollution be present, fermentation is uneven, fermentation Time is grown, and fermentation gained ginkgo leaf has the problems such as bilgy odour.

The content of the invention

It is an object of the invention to provide a kind of gingkgo health care fermented tea, composition based on large leaf sun withering tea, ginkgo, Also using STEVIA REBAUDIANA, P. kingianum, bitter buckwheat, jujube combination co-fermentation, ginkgo leave tea flavour and freshness can be increased, reduce ginkgo leaf Bitter taste, entrance time is sweet, is easy to be accepted, and pharmacological research shows, its protective effect to cardiovascular and cerebrovascular is relative to ginkgo Leaf is stronger；Meanwhile the present invention also provides a kind of method for preparing the gingkgo health care fermented tea, this method is by optimizing work of fermenting Skill, toxic component ginkgo acid content in ginkgo leaf can be significantly reduced, increase utilization rate of active components, improve its security.

The purpose of the present invention is achieved by the following technical programs：

A kind of gingkgo health care fermented tea, is made up of the raw material of following weight parts：100~300 parts of large leaf sun withering tea, ginkgo 50~300 parts of leaf, 20~100 parts of STEVIA REBAUDIANA, 10~50 parts of P. kingianum, 10~50 parts of bitter buckwheat, 10~50 parts of jujube.

The preparation method of gingkgo health care fermented tea of the present invention comprises the following steps：

1st, the P. kingianum of above-mentioned recipe quantity, bitter buckwheat, jujube are extracted with 60%~80% ethanol respectively, and extract solution concentrates respectively Be 1.20 ± 0.05 to relative density, the extraction concentrate of three mixed, and be dissolved in large leaf sun withering tea, ginkgo leaf and STEVIA REBAUDIANA for gross weight 50%~100% pure water in, it is standby as fermented tea absorbent solution；

2nd, the STEVIA REBAUDIANA of recipe quantity uses steam sterilizing, removes weeding fishy smell, standby after cooling；The ginkgo leaf of recipe quantity is washed Only, dry, it is standby；

3rd, by the fermented tea absorbent solution of step 1, the large leaf sun withering tea with recipe quantity, the STEVIA REBAUDIANA of step 2, ginkgo leaf Tealeaves mixture is mixed in proportion into, fully absorbs it；

4th, 25 DEG C -35 DEG C, relative humidity 60%-90% of fermenting house temperature is set, the tealeaves mixture of step 3 is placed in hair Into pile fermentation, pile fermentation is highly controlled in 50~100cm in ferment room, is carried out within the 1st~5 day first time turning, is carried out within the 6th~10 day second Turning, carried out a turning every 4~6 days later, and since the third time turning every time by raw material gross weight 20%~ 80% moisturizing, and the central temperature of fermented tea pile fermentation is controlled between 50~58 DEG C；

5th, continuing fermentation is after 20~50 days, and fermented tea pile fermentation is made thinner naturally dry or drying, and control moisture 8~ 12%, produce gingkgo health care fermented tea.

Wherein, gingkgo health care fermented tea, it preferably is selected from being made up of the raw material of following weight parts：200 parts of large leaf sun withering tea, silver 100 parts of apricot leaf, 50 parts of STEVIA REBAUDIANA, 20 parts of P. kingianum, 20 parts of bitter buckwheat, 20 parts of jujube.

The preparation method of gingkgo health care fermented tea of the present invention, can further it optimize as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 70% ethanol respectively, and extract solution is concentrated into relative density respectively For 1.20, the extraction concentrate of three is mixed, and is dissolved in using large leaf sun withering tea, ginkgo leaf and STEVIA REBAUDIANA as gross weight It is standby as fermented tea absorbent solution in 85% pure water；

4th, set 30 DEG C of fermenting house temperature, relative humidity 80%, the tealeaves mixture of step 3 is placed in fermenting house into wet Heap, pile fermentation are highly controlled in 50cm, carry out within the 3rd day first time turning, second of turning are carried out within the 8th day, later every 4~6 days A turning is carried out, and every time by 50% moisturizing of raw material gross weight since the third time turning, and control fermented tea pile fermentation Central temperature is at 55 DEG C；

5th, continuing fermentation is after 30 days, and fermented tea pile fermentation is made thinner naturally dry or drying, moisture 10% is controlled, produces Gingkgo health care fermented tea.

The ginkgo fermented tea of the present invention can be further prepared into loose tea, compressed tea, instant tea, tea beverage using conventional method Or tea bag.

Relative to prior art, the present invention has advantages below：

1st, the preparation method of gingkgo health care fermented tea provided by the invention, there is some following advantage：

A, mixed using ginkgo leaf with large leaf sun withering tea, STEVIA REBAUDIANA, while add P. kingianum, bitter buckwheat and Fructus Jujubae extract Combined fermentation mode, enrich the nutriment of fermented tea pile fermentation, compared with conventional Pu'er tea zymotechnique, this preparation method adds The fast curing of Pu'er tea, shortens fermentation time.

B, STEVIA REBAUDIANA, tartary buckwheat extract, Fructus Jujubae extract can improve the bitter taste of ginkgo leaf, while abundant mouthfeel, system It is mellow to obtain ginkgo leaf tea flavour.

C, by the central temperature to fermented tea pile fermentation and the optimization of fermentation time, the content of ginkgoic acid is effectively controlled, And active component lactone is remained to greatest extent.

2nd, compared with traditional ginkgo leave tea, gingkgo health care fermented tea of the invention is after everfermentation, the content drop of ginkgoic acid Low about 80%；Not only greatly reduce the content of toxic component；Meanwhile the molten of ginkgoic acid is also controlled in brewing process Go out, improve the security of product.Discovery, traditional ginkgo leave tea dissolution are measured to the ginkgoic acid brewed in rear solution About 10~20ppm ginkgoic acids, and gingkgo health care fermented tea dissolution about 1~2ppm of the present invention.

3rd, this process of fermenting in preparation method of the present invention has the work of enhancing P. kingianum ethanol extract and ginkgo leaf Blood stagnation resolvation, the synergy for protecting cardiovascular and cerebrovascular；Therefore after the gingkgo health care fermented tea of the invention prepared brews, because the aqueous solution omits Band is acid, is more beneficial for the dissolution of ginkgolides, improves the healthcare function of fermented tea.

Embodiment

Embodiment 1

Gingkgo health care fermented tea in all embodiments carries out the detection of following index：

1st, the said goods are subjected to sensory evaluation classification and measure Tea Polyphenols, crude fiber content according to GB/T2111-2008；

2nd, the total content measure of theaflavin, thearubigin and theabrownin is carried out；

3rd, finished product ginkgoic acid and lactone content measure are carried out；

4th, finished product is brewed 5 times with boiling water, merges the solution that brews of 5 times, and measure brews ginkgoic acid and lactone content in solution Measure.

The large leaf sun withering tea ratio of embodiment 2

(1), gingkgo health care fermented tea prescription 1 and preparation method

Gingkgo health care fermented tea prescription 1：Large leaf shines green tea 100kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 70% ethanol, and extract solution is concentrated into relative density and is respectively 1.20, the extraction concentrate of three is mixed, and be dissolved in 250L pure water, it is standby as fermented tea absorbent solution；

3rd, by the fermented tea absorbent solution of step 1, the large leaf sun withering tea with recipe quantity, the STEVIA REBAUDIANA of step volume, ginkgo Leaf mixes in proportion into tealeaves mixture, fully absorbs it；

(2), gingkgo health care fermented tea prescription 2 and preparation method

Gingkgo health care fermented tea prescription 2：Large leaf sun withering tea 200kg, take ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Preparation method is only the weight of large leaf sun withering tea by 100kg with the preparation method of gingkgo health care fermented tea prescription 1 It is replaced by 200kg.

(3), gingkgo health care fermented tea prescription 3 and preparation method

Gingkgo health care fermented tea prescription 3：Large leaf sun withering tea 300kg, take ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Preparation method is only the weight of large leaf sun withering tea by 100kg with the preparation method of gingkgo health care fermented tea prescription 1 It is replaced by 300kg.

From the point of view of the result of table 1, large leaf sun withering tea is sampled in 100~300kg, and the various indexs of tea are similar, with 200kg is slightly good.

The large leaf sun withering tea ratiometric result of table 1

Note：Ginkgo acid content in raw material ginkgo leaf is 2.15%, lactone content 0.33%；The above results are to convert extremely The weight of ginkgo leaf, rather than the gross weight of all raw materials.

The ginkgo leaf ratio of embodiment 3

(1), gingkgo health care fermented tea prescription 4 and preparation method

Gingkgo health care fermented tea prescription 4：Large leaf shines green tea 200kg, ginkgo leaf 50kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 70% ethanol, and extract solution is concentrated into relative density and is respectively 1.20, the extraction concentrate of three is mixed, and be dissolved in 300L pure water, it is standby as fermented tea absorbent solution；

(2), gingkgo health care fermented tea prescription 5 and preparation method

Gingkgo health care fermented tea prescription 5：Large leaf sun withering tea 200kg, take ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Preparation method is only that the weight of ginkgo leaf is replaced by by 50kg with the preparation method of gingkgo health care fermented tea prescription 4 100kg。

(3), gingkgo health care fermented tea prescription 6 and preparation method

Gingkgo health care fermented tea prescription 6：Large leaf sun withering tea 200kg, take ginkgo leaf 300kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Preparation method is only that the weight of ginkgo leaf is replaced by by 50kg with the preparation method of gingkgo health care fermented tea prescription 4 300kg。

The ginkgo leaf ratiometric result of table 2

Note：Ginkgo acid content in raw material ginkgo leaf is 2.15%, lactone content 0.33%；The above results are to convert extremely The weight of ginkgo leaf, rather than the gross weight of all raw materials

The STEVIA REBAUDIANA ratio of embodiment 4

(1), gingkgo health care fermented tea prescription 7 and preparation method

Gingkgo health care fermented tea prescription 7：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 20kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

(2), gingkgo health care fermented tea prescription 8 and preparation method

Gingkgo health care fermented tea prescription 8：Large leaf sun withering tea 200kg, take ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Preparation method is only that the weight of STEVIA REBAUDIANA is replaced by by 20kg with the preparation method of gingkgo health care fermented tea prescription 7 50kg。

(3), gingkgo health care fermented tea prescription 9 and preparation method

Gingkgo health care fermented tea prescription 9：Large leaf sun withering tea 200kg, take ginkgo leaf 300kg, STEVIA REBAUDIANA 100kg, Yunnan yellow Smart 20kg, bitter buckwheat 20kg, jujube 20kg.

Preparation method is only that the weight of STEVIA REBAUDIANA is replaced by by 20kg with the preparation method of gingkgo health care fermented tea prescription 7 100kg。

From the point of view of the result of table 3, STEVIA REBAUDIANA is sampled in 20~100kg, and the various indexs of tea are similar, omited with 50kg It is good.

The STEVIA REBAUDIANA ratiometric result of table 3

The P. kingianum ratio of embodiment 5

(1), gingkgo health care fermented tea prescription 10 and preparation method

Gingkgo health care fermented tea prescription 10：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 10kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

(2), gingkgo health care fermented tea prescription 11 and preparation method

Gingkgo health care fermented tea prescription 11：Large leaf sun withering tea 200kg, take ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, Yunnan yellow Smart 20kg, bitter buckwheat 20kg, jujube 20kg.

Preparation method is only that the weight of P. kingianum is replaced by by 10kg with the preparation method of gingkgo health care fermented tea prescription 10 20kg。

(3), gingkgo health care fermented tea prescription 12 and preparation method

Gingkgo health care fermented tea prescription 12：Large leaf sun withering tea 200kg, take ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, Yunnan yellow Smart 50kg, bitter buckwheat 20kg, jujube 20kg.

Preparation method is only that the weight of P. kingianum is replaced by by 10kg with the preparation method of gingkgo health care fermented tea prescription 10 50kg。

From the point of view of the result of table 4, P. kingianum is sampled in 10~50kg, and the various indexs of tea are similar, slightly good with 20kg.

The P. kingianum ratiometric result of table 4

The bitter buckwheat ratio of embodiment 6

(1), gingkgo health care fermented tea prescription 13 and preparation method

Gingkgo health care fermented tea prescription 13：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 10kg, jujube 20kg.

Its preparation method is as follows：

(2), gingkgo health care fermented tea prescription 14 and preparation method

Gingkgo health care fermented tea prescription 14：Large leaf sun withering tea 200kg, take ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, Yunnan yellow Smart 20kg, bitter buckwheat 20kg, jujube 20kg.

Preparation method is only that the weight of bitter buckwheat is replaced by by 10kg with the preparation method of gingkgo health care fermented tea prescription 13 20kg。

(3), gingkgo health care fermented tea prescription 15 and preparation method

Gingkgo health care fermented tea prescription 15：Large leaf sun withering tea 200kg, take ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, Yunnan yellow Smart 20kg, bitter buckwheat 50kg, jujube 20kg.

Preparation method is only that the weight of bitter buckwheat is replaced by by 10kg with the preparation method of gingkgo health care fermented tea prescription 13 50kg。

From the point of view of the result of table 5, bitter buckwheat is sampled in 10~50kg, and the various indexs of tea are similar, slightly good with 20kg.

The bitter buckwheat ratiometric result of table 5

The jujube ratio of embodiment 7

(1), gingkgo health care fermented tea prescription 16 and preparation method

Gingkgo health care fermented tea prescription 16：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 10kg.

Its preparation method is as follows：

(2), gingkgo health care fermented tea prescription 17 and preparation method

Gingkgo health care fermented tea prescription 17：Large leaf sun withering tea 200kg, take ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, Yunnan yellow Smart 20kg, bitter buckwheat 20kg, jujube 20kg.

Preparation method is only that the weight of jujube is replaced by by 10kg with the preparation method of gingkgo health care fermented tea prescription 16 20kg。

(3), gingkgo health care fermented tea prescription 18 and preparation method

Gingkgo health care fermented tea prescription 18：Large leaf sun withering tea 200kg, take ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, Yunnan yellow Smart 20kg, bitter buckwheat 20kg, jujube 50kg.

Preparation method is only that the weight of jujube is replaced by by 10kg with the preparation method of gingkgo health care fermented tea prescription 16 50kg。

From the point of view of the result of table 6, jujube is sampled in 10~50kg, and the various indexs of tea are similar, slightly good with 20kg.

The jujube ratiometric result of table 6

The proportion of ethanol of embodiment 8

(1), gingkgo health care fermented tea prescription 19 and preparation method

Gingkgo health care fermented tea prescription 19：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 60% ethanol, and extract solution is concentrated into relative density and is respectively 1.20, the extraction concentrate of three is mixed, and be dissolved in 300L pure water, it is standby as fermented tea absorbent solution；

(2), gingkgo health care fermented tea prescription 20 and preparation method

Gingkgo health care fermented tea prescription 20：With gingkgo health care fermented tea prescription 19.

Its preparation method is as follows：

Preparation method 2~5 is only that proportion of ethanol is replaced by by 60% with the preparation method of gingkgo health care fermented tea prescription 19 70%.

(3), gingkgo health care fermented tea prescription 21 and preparation method

Gingkgo health care fermented tea prescription 21：With gingkgo health care fermented tea prescription 19.

Its preparation method is as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 80% ethanol, and extract solution is concentrated into relative density and is respectively 1.20, the extraction concentrate of three is mixed, and be dissolved in 300L pure water, it is standby as fermented tea absorbent solution；

Preparation method 2~5 is only that proportion of ethanol is replaced by by 60% with the preparation method of gingkgo health care fermented tea prescription 19 80%.

From the point of view of the result of table 7, for concentration of alcohol at 60~80%, the various indexs of tea are similar.

The proportion of ethanol result of table 7

The extract solution of embodiment 9 concentrates relative density

(1), gingkgo health care fermented tea prescription 22 and preparation method

Gingkgo health care fermented tea prescription 22：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 70% ethanol, and extract solution is concentrated into relative density and is respectively 1.15, the extraction concentrate of three is mixed, and be dissolved in 300L pure water, it is standby as fermented tea absorbent solution；

(2), gingkgo health care fermented tea prescription 23 and preparation method

Gingkgo health care fermented tea prescription 23：With gingkgo health care fermented tea prescription 22.

Its preparation method is as follows：

Preparation method 2~5 with the preparation method of gingkgo health care fermented tea prescription 22, be only extract solution concentration relative density by 1.15 it is replaced by 1.20.

(3), gingkgo health care fermented tea prescription 24 and preparation method

Gingkgo health care fermented tea prescription 24：With gingkgo health care fermented tea prescription 22.

Its preparation method is as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 70% ethanol, and extract solution is concentrated into relative density and is respectively 1.25, the extraction concentrate of three is mixed, and be dissolved in 300L pure water, it is standby as fermented tea absorbent solution；

Preparation method 2~5 with the preparation method of gingkgo health care fermented tea prescription 22, be only extract solution concentration relative density by 1.15 it is replaced by 1.25.

From the point of view of the result of table 8, extract solution concentrates relative density at 1.15~1.25, and the various indexs of tea are similar.

The extract solution of table 8 concentrates relative density ratiometric result

The fermented tea absorbent solution volume of embodiment 10

(1), gingkgo health care fermented tea prescription 25 and preparation method

Gingkgo health care fermented tea prescription 25：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 70% ethanol, and extract solution is concentrated into relative density and is respectively 1.20, the extraction concentrate of three is mixed, and be dissolved in 175L pure water, correspond to large leaf sun withering tea, ginkgo leaf and The 50% of STEVIA REBAUDIANA gross weight, it is standby as fermented tea absorbent solution；

(2), gingkgo health care fermented tea prescription 26 and preparation method

Gingkgo health care fermented tea prescription 26：With gingkgo health care fermented tea prescription 25.

Its preparation method is as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 70% ethanol, and extract solution is concentrated into relative density and is respectively 1.20, the extraction concentrate of three is mixed, and be dissolved in 262.5L pure water, correspond to large leaf sun withering tea, ginkgo leaf It is standby as fermented tea absorbent solution with the 75% of STEVIA REBAUDIANA gross weight；

Preparation method 2~5 with the preparation method of gingkgo health care fermented tea prescription 25, be only fermented tea absorbent solution volume by 175L is replaced by 262.5L.

(3), gingkgo health care fermented tea prescription 27 and preparation method

Gingkgo health care fermented tea prescription 27：With gingkgo health care fermented tea prescription 25.

Its preparation method is as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 70% ethanol, and extract solution is concentrated into relative density and is respectively 1.20, the extraction concentrate of three is mixed, and be dissolved in 350L pure water, correspond to large leaf sun withering tea, ginkgo leaf and The 100% of STEVIA REBAUDIANA gross weight, it is standby as fermented tea absorbent solution；

Preparation method 2~5 with the preparation method of gingkgo health care fermented tea prescription 25, be only fermented tea absorbent solution volume by 175L is replaced by 350L.

From the point of view of the result of table 9, fermented tea absorbent solution volume and large leaf sun withering tea, ginkgo leaf and STEVIA REBAUDIANA gross weight ratio For value at 50%~100%, the various indexs of tea are similar.

The fermented tea absorbent solution volume result of table 9

The fermenting house temperature of embodiment 11 (fermented tea pile fermentation central temperature)

(1), gingkgo health care fermented tea prescription 28 and preparation method

Gingkgo health care fermented tea prescription 28：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

1st, the P. kingianum of recipe quantity, bitter buckwheat, jujube are extracted with 70% ethanol, and extract solution is concentrated into relative density and is respectively 1.20, the extraction concentrate of three is mixed, and be dissolved in 300L pure water, correspond to large leaf sun withering tea, ginkgo leaf and The 50% of STEVIA REBAUDIANA gross weight, it is standby as fermented tea absorbent solution；

4th, set 25 DEG C of fermenting house temperature, relative humidity 80%, the tealeaves mixture of step 3 is placed in fermenting house into wet Heap, pile fermentation are highly controlled in 50cm, carry out within the 3rd day first time turning, second of turning are carried out within the 8th day, later every 4~6 days A turning is carried out, and every time by 50% moisturizing of raw material gross weight since the third time turning, and control fermented tea pile fermentation Central temperature is at 52 DEG C；

(2), gingkgo health care fermented tea prescription 29 and preparation method

Gingkgo health care fermented tea prescription 29：With gingkgo health care fermented tea prescription 28.

Its preparation method is as follows：

Preparation method 1~3,5 with the preparation method of gingkgo health care fermented tea prescription 28, be only fermenting house temperature by 25 DEG C more 30 DEG C are changed to, the central temperature of fermented tea pile fermentation is replaced by 55 DEG C by 52 DEG C.

(3), gingkgo health care fermented tea prescription 30 and preparation method

Gingkgo health care fermented tea prescription 30：With gingkgo health care fermented tea prescription 28.

Its preparation method is as follows：

4th, set 35 DEG C of fermenting house temperature, relative humidity 80%, the tealeaves mixture of step 3 is placed in fermenting house into wet Heap, pile fermentation are highly controlled in 50cm, carry out within the 3rd day first time turning, second of turning are carried out within the 8th day, later every 4~6 days A turning is carried out, and every time by 50% moisturizing of raw material gross weight since the third time turning, and control fermented tea pile fermentation Central temperature is at 58 DEG C；

Preparation method 1~3,5 with the preparation method of gingkgo health care fermented tea prescription 28, be only fermenting house temperature by 25 DEG C more 35 DEG C are changed to, the central temperature of fermented tea pile fermentation is replaced by 58 DEG C by 52 DEG C.

From the point of view of the result of table 10, influence of the fermented tea heap central temperature for ginkgo fermented tea quality is very big, temperature 52 When DEG C (25 DEG C of fermenting house temperature) or 58 DEG C (35 DEG C of fermenting house temperature), ginkgo fermented tea is classified as 5 grades to 3 by sensory evaluation Between level, quality is less than 55 DEG C (30 DEG C of fermenting house temperature).During 52 DEG C of fermented tea heap central temperature, ginkgo acid content is far above hair Ferment tea heap central temperature 55 and 58 DEG C,；During 58 DEG C of fermented tea heap central temperature, lactone content is far below fermented tea heap central temperature 52 and 55 DEG C, all in all, 55 DEG C of fermented tea heap central temperature is optimal.

The fermenting house temperature of table 10 (fermented tea heap central temperature) result

The fermenting house relative humidity of embodiment 12 (fermented tea heap central temperature)

(1), gingkgo health care fermented tea prescription 31 and preparation method

Gingkgo health care fermented tea prescription 31：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

4th, set 30 DEG C of fermenting house temperature, relative humidity 60%, the tealeaves mixture of step 3 is placed in fermenting house into wet Heap, pile fermentation are highly controlled in 50cm, carry out within the 3rd day first time turning, second of turning are carried out within the 8th day, later every 4~6 days A turning is carried out, and every time by 50% moisturizing of raw material gross weight since the third time turning, and control fermented tea pile fermentation Central temperature is at 50 DEG C；

(2), gingkgo health care fermented tea prescription 32 and preparation method

Gingkgo health care fermented tea prescription 32：With gingkgo health care fermented tea prescription 31.

Its preparation method is as follows：

4th, set 30 DEG C of fermenting house temperature, relative humidity 70%, the tealeaves mixture of step 3 is placed in fermenting house into wet Heap, pile fermentation are highly controlled in 50cm, carry out within the 3rd day first time turning, second of turning are carried out within the 8th day, later every 4~6 days A turning is carried out, and every time by 50% moisturizing of raw material gross weight since the third time turning, and control fermented tea pile fermentation Central temperature is at 53 DEG C；

Preparation method 1~3,5 with the preparation method of gingkgo health care fermented tea prescription 31, be only fermenting house relative humidity by 60% is replaced by 70%, and the central temperature of fermented tea pile fermentation is replaced by 53 DEG C by 50 DEG C.

(3), gingkgo health care fermented tea prescription 33 and preparation method

Gingkgo health care fermented tea prescription 33：With gingkgo health care fermented tea prescription 31.

Its preparation method is as follows：

Preparation method 1~3,5 with the preparation method of gingkgo health care fermented tea prescription 31, be only fermenting house relative humidity by 60% is replaced by 80%, and the central temperature of fermented tea pile fermentation is replaced by 55 DEG C by 50 DEG C.

(4), gingkgo health care fermented tea prescription 34 and preparation method

Gingkgo health care fermented tea prescription 34：With gingkgo health care fermented tea prescription 31.

Its preparation method is as follows：

4th, set 30 DEG C of fermenting house temperature, relative humidity 90%, the tealeaves mixture of step 3 is placed in fermenting house into wet Heap, pile fermentation are highly controlled in 50cm, carry out within the 3rd day first time turning, second of turning are carried out within the 8th day, later every 4~6 days A turning is carried out, and every time by 50% moisturizing of raw material gross weight since the third time turning, and control fermented tea pile fermentation Central temperature is at 57 DEG C；

Preparation method 1~3,5 with the preparation method of gingkgo health care fermented tea prescription 31, be only fermenting house relative humidity by 60% is replaced by 90%, and the central temperature of fermented tea pile fermentation is replaced by 57 DEG C by 50 DEG C.

Fermenting house relative humidity is 70%, and finished product ginkgo acid content is of a relatively high；Fermenting house relative humidity is 90%, finished product Lactone content declines to a great extent；From the point of view of relatively, fermenting house relative humidity is 80% (55 DEG C of fermented tea heap central temperature), fermentation effect Fruit is optimal.

The fermenting house relative humidity of table 11 (fermented tea heap central temperature) result

The pile fermentation of embodiment 13 height

(1), gingkgo health care fermented tea prescription 35 and preparation method

Gingkgo health care fermented tea prescription 35：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

(2), gingkgo health care fermented tea prescription 36 and preparation method

Gingkgo health care fermented tea prescription 36：With gingkgo health care fermented tea prescription 35.

Its preparation method is as follows：

4th, set 30 DEG C of fermenting house temperature, relative humidity 80%, the tealeaves mixture of step 3 is placed in fermenting house into wet Heap, pile fermentation are highly controlled in 75cm, carry out within the 3rd day first time turning, second of turning are carried out within the 8th day, later every 4~6 days A turning is carried out, and every time by 50% moisturizing of raw material gross weight since the third time turning, and control fermented tea pile fermentation Central temperature is at 55 DEG C；

Preparation method 1~3,5 is only that pile fermentation height is changed by 50cm with the preparation method of gingkgo health care fermented tea prescription 35 For 75cm.

(3), gingkgo health care fermented tea prescription 37 and preparation method

Gingkgo health care fermented tea prescription 37：With gingkgo health care fermented tea prescription 35.

Its preparation method is as follows：

4th, set 30 DEG C of fermenting house temperature, relative humidity 80%, the tealeaves mixture of step 3 is placed in fermenting house into wet Heap, pile fermentation are highly controlled in 100cm, carry out within the 3rd day first time turning, second of turning are carried out within the 8th day, later every 4~6 days A turning is carried out, and every time by 50% moisturizing of raw material gross weight since the third time turning, and control fermented tea pile fermentation Central temperature is at 55 DEG C；

Preparation method 1~3,5 is only that pile fermentation height is changed by 50cm with the preparation method of gingkgo health care fermented tea prescription 35 For 100cm.

From the point of view of the result of table 12, pile fermentation height is preferably controlled in 50~75cm.

The pile fermentation height results of table 12

The rate of water make-up of embodiment 14

(1), gingkgo health care fermented tea prescription 38 and preparation method

Gingkgo health care fermented tea prescription 38：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

4th, set 30 DEG C of fermenting house temperature, relative humidity 80%, the tealeaves mixture of step 3 is placed in fermenting house into wet Heap, pile fermentation are highly controlled in 50cm, carry out within the 3rd day first time turning, second of turning are carried out within the 8th day, later every 4~6 days A turning is carried out, and every time by 20% moisturizing of raw material gross weight since the third time turning, and control fermented tea pile fermentation Central temperature is at 55 DEG C；

(2), gingkgo health care fermented tea prescription 39 and preparation method

Gingkgo health care fermented tea prescription 39：With gingkgo health care fermented tea prescription 38.

Its preparation method is as follows：

Preparation method 1~3,5 is only that rate of water make-up is replaced by by 20% with the preparation method of gingkgo health care fermented tea prescription 38 50%.

(3), gingkgo health care fermented tea prescription 40 and preparation method

Gingkgo health care fermented tea prescription 40：With gingkgo health care fermented tea prescription 38.

Its preparation method is as follows：

4th, set 30 DEG C of fermenting house temperature, relative humidity 80%, the tealeaves mixture of step 3 is placed in fermenting house into wet Heap, pile fermentation are highly controlled in 50cm, carry out within the 3rd day first time turning, second of turning are carried out within the 8th day, later every 4~6 days A turning is carried out, and every time by 80% moisturizing of raw material gross weight since the third time turning, and control fermented tea pile fermentation Central temperature is at 55 DEG C；

Preparation method 1~3,5 is only that rate of water make-up is replaced by by 20% with the preparation method of gingkgo health care fermented tea prescription 35 80%.

From the point of view of the result of table 13, rate of water make-up is preferably controlled 50%~80%.

The rate of water make-up result of table 13

The fermentation time of embodiment 15

(1), gingkgo health care fermented tea prescription 41 and preparation method

Gingkgo health care fermented tea prescription 41：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

5th, continuing fermentation is after 20 days, and fermented tea pile fermentation is made thinner naturally dry or drying, moisture 10% is controlled, produces Gingkgo health care fermented tea.

(2), gingkgo health care fermented tea prescription 42 and preparation method

Gingkgo health care fermented tea prescription 42：With gingkgo health care fermented tea prescription 41.

Its preparation method is as follows：

Preparation method 1~4 is only that continuing fermentation was replaced by by 20 days with the preparation method of gingkgo health care fermented tea prescription 41 30 days.

(3), gingkgo health care fermented tea prescription 43 and preparation method

Gingkgo health care fermented tea prescription 43：With gingkgo health care fermented tea prescription 41.

Its preparation method is as follows：

5th, continuing fermentation is after 40 days, and fermented tea pile fermentation is made thinner naturally dry or drying, moisture 10% is controlled, produces Gingkgo health care fermented tea.

Preparation method 1~4 is only that continuing fermentation was replaced by by 20 days with the preparation method of gingkgo health care fermented tea prescription 41 40 days.

(4), gingkgo health care fermented tea prescription 44 and preparation method

Gingkgo health care fermented tea prescription 44：With gingkgo health care fermented tea prescription 41.

Its preparation method is as follows：

5th, continuing fermentation is after 50 days, and fermented tea pile fermentation is made thinner naturally dry or drying, moisture 10% is controlled, produces Gingkgo health care fermented tea.

Preparation method 1~4 is only that continuing fermentation was replaced by by 20 days with the preparation method of gingkgo health care fermented tea prescription 41 50 days.

From the point of view of the result of table 14, fermentation time was preferably controlled at 30~40 days.

The fermentation time result of table 14

The moisture content of finished products content of embodiment 16

(1), gingkgo health care fermented tea prescription 45 and preparation method

Gingkgo health care fermented tea prescription 45：Large leaf shines green tea 200kg, ginkgo leaf 100kg, STEVIA REBAUDIANA 50kg, P. kingianum 20kg, bitter buckwheat 20kg, jujube 20kg.

Its preparation method is as follows：

5th, continuing fermentation is after 30 days, and fermented tea pile fermentation is made thinner naturally dry or drying, moisture 8% is controlled, produces Gingkgo health care fermented tea.

(2), gingkgo health care fermented tea prescription 46 and preparation method

Gingkgo health care fermented tea prescription 46：With gingkgo health care fermented tea prescription 45.

Its preparation method is as follows：

Preparation method 1~4 is only that moisture is replaced by by 8% with the preparation method of gingkgo health care fermented tea prescription 45 10%.

(3), gingkgo health care fermented tea prescription 47 and preparation method

Gingkgo health care fermented tea prescription 47：With gingkgo health care fermented tea prescription 45.

Its preparation method is as follows：

5th, continuing fermentation is after 30 days, and fermented tea pile fermentation is made thinner naturally dry or drying, moisture 12% is controlled, produces Gingkgo health care fermented tea.

Preparation method 1~4 is only that moisture is replaced by by 8% with the preparation method of gingkgo health care fermented tea prescription 45 12%.

From the point of view of the result of table 15, moisture content of finished products content is controlled 8~12%.

The moisture content of finished products content results of table 15

Moisture content of finished products content	8%	10%	12%
				Sensory evaluation is classified	Three-level is to one-level	Three-level is to one-level	Three-level is to one-level
Polyphenol content (%)	13.4	13.5	13.5
				Crude fiber content (%)	12.3	12.3	12.2
Theaflavin, thearubigin and theabrownin total content (%)	17.8	17.8	17.7
				Finished product ginkgo acid content (%)	0.43	0.41	0.42
Finished product lactone content (%)	0.27	0.26	0.27
				Brew solution ginkgo acid content (PPM)	1.2	1.2	1.3
Brew solution lactone content (%)	0.16	0.16	0.16

The cardiovascular protective effect of the gingkgo health care fermented tea of embodiment 17

Medicine 1：Gingkgo health care fermented tea is prepared by the preparation method of gingkgo health care fermented tea prescription 46,100g is taken, with boiling Water brews 5 times, each 2L, merge it is all brews solution, be concentrated and dried, obtain 30g fermented tea extracts (dosage 60mg/kg, Conversion is 200mg/kg to crude drug, and ginkgoic acid dissolution is 1.3ppm).

Medicine 2：P. kingianum is lacked, other carry out preparing gingkgo health care hair with the preparation method of gingkgo health care fermented tea prescription 46 Ferment tea, takes 100g, is brewed 5 times, each 2L with boiling water, and merging is all to brew solution, is concentrated and dried, obtains 30g fermented tea extracts (dosage 60mg/kg, it is 200mg/kg to convert to crude drug, and ginkgoic acid dissolution is 1.4ppm)

Medicine 3：Traditional ginkgo leave tea (i.e. ginkgo leaf), take 100g, are brewed 5 times, each 2L with boiling water, merge all brew Solution, it is concentrated and dried, obtaining 30g ginkgo biloba p.es, (dosage 60mg/kg, it is 200mg/kg to convert to crude drug, ginkgoic acid Dissolution is 18.9ppm)

Medicine 4：Ginkgo biloba p.e (dosage 40mg/kg, it is 200mg/kg to convert to crude drug)

Male SD rat is randomly divided into 6 groups, model group, sham-operation group, medicine 1, medicine 2, medicine 3, medicine 4；Medicine 1, 2nd, 3,4 groups carry out gastric infusion for three days on end, and model group and sham-operation group give the distilled water of same volume.After administration three days, greatly After mouse is weighed, the anesthesia of 20% urethane is injected intraperitoneally by 1g/kg, dorsal position is fixed, and four limbs are subcutaneously inserted needle electrode, with monitoring The lead electrocardiogram of standard limb II.Neck median incision, separate tracheae and be intubated, connect miniature artificial respirator, it is left along breastbone Edge the 3rd, 4 intercostals open thoracic cavity, carefully tear pericardium, exposure heart, with the left coronal of left auricle of heart pulmonary conus intersection Vein is mark, standby away from being threaded at left auricle of heart lower edge 2mm in coronary artery anterior descending branch with small round needle and No. 5 non-traumatic sewing threads One is put with, line center and is about 0.6cm, the small plastic tube of diameter 0.1cm hard, and small plastic tube is just pressed in arteria coroaria sinistra during ligation On descending anterior branch, to block and recover blood flow, substantially raised with electrocardiogram S-T segment to ligature successful standard (sham-operation after ligation Group does not ligature).Small plastic tube Reperfu- sion 60min is pulled out after ligation 30min.Reperfu- sion takes rat heart after terminating, section, 37 DEG C 30min is incubated in 1%TTC solution, calculating myocardium Infarct area after 4h is fixed in 10% neutral formalin.

The result of table 16 can obtain, after ginkgo leaf is fermented, still with cardiovascular protective effect, and with P. kingianum alcohol extract Ferment together, cardiovascular protective effect substantially increases, even better than ginkgo biloba p.e.

The cardiovascular protective effect of the gingkgo health care fermented tea of table 16

Group	n	Infarct size (%)
			Sham-operation group	8	0
Model group	8	30.52±4.76
			1 group of medicine	8	17.24±2.43**^##$$&
2 groups of medicine	8	23.71±4.30**
			3 groups of medicine	8	24.55±4.16*
4 groups of medicine	8	21.68±3.19**

With model group ratio：*：P<0.05；**：P<0.01；

1 group of medicine and 2 groups of ratios of medicine：##：P<0.05.

1 group of medicine and 3 groups of ratios of medicine：$$<0.05.

1 group of medicine and 4 groups of ratios of medicine：&<0.05

The cerebrovascular protective effect of the gingkgo health care fermented tea of embodiment 18

Medicine 1：Gingkgo health care fermented tea is prepared by the preparation method of gingkgo health care fermented tea prescription 46,100g is taken, with boiling Water brews 5 times, each 2L, and merging is all to brew solution, is concentrated and dried, obtains 30g fermented tea extracts (dosage 90mg/ Kg, it is 300mg/kg to convert to crude drug, and ginkgoic acid dissolution is 1.3ppm)

Medicine 2：P. kingianum is lacked, the preparation method of other gingkgo health care fermented tea prescriptions 46 prepares gingkgo health care fermented tea, 100g is taken, is brewed 5 times, each 2L with boiling water, merging is all to brew solution, is concentrated and dried, and obtains (the administration of 30g fermented tea extracts Dosage 90mg/kg, it is 300mg/kg to convert to crude drug, and ginkgoic acid dissolution is 1.4ppm)

Medicine 3：Traditional ginkgo leave tea (i.e. ginkgo leaf), take 100g, are brewed 5 times, each 2L with boiling water, merge it is all brew it is molten Liquid, it is concentrated and dried, obtaining 30g extracts, (dosage 90mg/kg, it is 300mg/kg to convert to crude drug, and ginkgoic acid dissolution is 18.9ppm)

Medicine 4：Ginkgo biloba p.e (dosage 60mg/kg, it is 300mg/kg to convert to crude drug)

Male ICR mouse is randomly divided into 6 groups, model group, sham-operation group, medicine 1, medicine 2, medicine 3, medicine 4；Medicine 1, 2nd, 3,4 groups carry out gastric infusion for three days on end, and model group and sham-operation group give the distilled water of same volume.It is small after administration three days After mouse is weighed, 4% chloral hydrate anesthesia, the sterilization of skin of neck unhairing, median incision, separation left side is injected intraperitoneally by 400mg/kg Arteria carotis communis and external carotid artery.External carotid artery is ligatured, an osculum is cut off on arteria carotis communis, a head end is burnt to globulate A diameter of 0.11mm fishing line is inserted to arteria cerebri media crotch, insertion depth is about through internal carotid along arteria carotis communis to encephalic 12~13mm, ligature internal carotid and arteria carotis communis, fixed fishing line, skin suture.Ischemic gently pulls out fishing line to neck after 2 hours At inside and outside aortic bifurcation, Reperfu- sion is realized.Sham-operation group only separates arteria carotis communis and external carotid artery, does not insert fishing line.Mouse revives It is that model is successfully prepared to occur operation contralateral limbs dyskinesia afterwards.Neurological deficit scoring is commented with reference to Longa 5 points of systems Minute mark is accurate to carry out neurological deficit scoring.0 point is normal, impassivity injury symptoms；Before l points is are unable to full extension offside Pawl；2 points are to be turn-taked after carrying tail to outside；3 points are toppled over when being walking to offside；4 points is can not spontaneous walking or the loss of consciousness.Hand By mouse sacrificed by decapitation, brain is taken within postoperative 24 hours, the SOD calculated in brain infarction area percentage, and measure brain tissue homogenate lives Property and MDA contents.Concrete outcome is shown in Table 8.

The result of table 17 can obtain, after ginkgo leaf is fermented, still with cerebrovascular protective effect, and with P. kingianum alcohol extract Ferment together, cerebrovascular protective effect substantially increases, even better than ginkgo biloba p.e.

The cerebrovascular protective effect of the gingkgo health care fermented tea of table 17

With model group ratio：*：P<0.05；**：P<0.01；

1 group of medicine and 2 groups of ratios of medicine：##：P<0.05.

1 group of medicine and 3 groups of ratios of medicine：$$<0.05.

1 group of medicine and 4 groups of ratios of medicine：&<0.05.

Claims

1. a kind of gingkgo health care fermented tea, is made up of the raw material of following weight parts：100~300 parts of large leaf sun withering tea, ginkgo leaf 50~300 parts, 20~100 parts of STEVIA REBAUDIANA, 10~50 parts of P. kingianum, 10~50 parts of bitter buckwheat, 10~50 parts of jujube.

2. a kind of gingkgo health care fermented tea as claimed in claim 1, is made up of the raw material of following weight parts：Large leaf sun withering 200 parts of tea, 100 parts of ginkgo leaf, 50 parts of STEVIA REBAUDIANA, 20 parts of P. kingianum, 20 parts of bitter buckwheat, 20 parts of jujube.

3. a kind of a kind of preparation method of gingkgo health care fermented tea as described in claims 1 or 2, specific preparation process is such as Under：

(1), the P. kingianum of above-mentioned recipe quantity, bitter buckwheat, jujube are extracted with 60%~80% ethanol respectively, and extract solution is concentrated into respectively Relative density is 1.20 ± 0.05, and the extraction concentrate of three is mixed, and is dissolved in large leaf sun withering tea, ginkgo leaf and sweet tea Ye Ju be gross weight 50%~100% pure water in, it is standby as fermented tea absorbent solution；

(2), the STEVIA REBAUDIANA of recipe quantity uses steam sterilizing, removes weeding fishy smell, standby after cooling；The ginkgo leaf of recipe quantity is cleaned, Drying, it is standby；

(3), by the fermented tea absorbent solution of step 1, the large leaf sun withering tea with recipe quantity, the STEVIA REBAUDIANA of step 2, ginkgo leaf are pressed Ratio is mixed into tealeaves mixture, fully absorbs it；

(4) 25 DEG C -35 DEG C, relative humidity 60%-90% of fermenting house temperature, is set, the tealeaves mixture of step 3 is placed in fermentation Into pile fermentation, pile fermentation is highly controlled in 50~100cm, is carried out first time turning within the 1st~5 day, is turned over for the second time within the 6th~10 day in room Heap, a turning was carried out every 4~6 days later, and every time by the 20%~80% of raw material gross weight since the third time turning Moisturizing, and the central temperature of fermented tea pile fermentation is controlled between 50~58 DEG C；

(5), continuing fermentation is after 20~50 days, and fermented tea pile fermentation is made thinner naturally dry or drying, and control moisture 8~ 12%, produce gingkgo health care fermented tea.

4. a kind of preparation method of gingkgo health care fermented tea as claimed in claim 3, it is characterised in that step 1) is recipe quantity P. kingianum, bitter buckwheat, jujube extracted respectively with 70% ethanol, extract solution is concentrated into relative density as 1.20 respectively, by three's Concentrate mixing is extracted, and is dissolved in using large leaf sun withering tea, ginkgo leaf and STEVIA REBAUDIANA as in the 85% of gross weight pure water, It is standby as fermented tea absorbent solution.

5. a kind of preparation method of gingkgo health care fermented tea as claimed in claim 3, it is characterised in that step 4) is sent out for setting 30 DEG C of ferment room temperature, relative humidity 80%, the tealeaves mixture of step 3 is placed in fermenting house and highly controlled into pile fermentation, pile fermentation 50cm, first time turning is carried out within the 3rd day, carry out second of turning within the 8th day, carried out a turning every 4~6 days later, and certainly Third time turning starts every time by 50% moisturizing of raw material gross weight, and controls the central temperature of fermented tea pile fermentation at 55 DEG C.

6. the preparation method of a kind of gingkgo health care fermented tea according to claim 3, it is characterised in that step 5) is lasting After fermentation 30 days, fermented tea pile fermentation is made thinner naturally dry or drying, controls moisture 10%, produces gingkgo health care fermentation Tea.