CN107586847A - 一种环形接头及其应用 - Google Patents
一种环形接头及其应用 Download PDFInfo
- Publication number
- CN107586847A CN107586847A CN201711039852.1A CN201711039852A CN107586847A CN 107586847 A CN107586847 A CN 107586847A CN 201711039852 A CN201711039852 A CN 201711039852A CN 107586847 A CN107586847 A CN 107586847A
- Authority
- CN
- China
- Prior art keywords
- sequence
- ring joint
- ion
- complementary
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000295 complement effect Effects 0.000 claims abstract description 21
- 238000002493 microarray Methods 0.000 claims abstract description 6
- 239000002773 nucleotide Substances 0.000 claims description 9
- 125000003729 nucleotide group Chemical group 0.000 claims description 9
- 230000004048 modification Effects 0.000 claims description 5
- 238000012986 modification Methods 0.000 claims description 5
- 230000026731 phosphorylation Effects 0.000 claims description 4
- 238000006366 phosphorylation reaction Methods 0.000 claims description 4
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 238000010276 construction Methods 0.000 abstract description 6
- 238000005859 coupling reaction Methods 0.000 abstract description 4
- 230000008878 coupling Effects 0.000 abstract description 2
- 238000010168 coupling process Methods 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 description 36
- 239000000523 sample Substances 0.000 description 36
- 239000002585 base Substances 0.000 description 18
- 108020004414 DNA Proteins 0.000 description 16
- 150000002500 ions Chemical class 0.000 description 16
- 238000012163 sequencing technique Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 10
- 238000001514 detection method Methods 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 238000000137 annealing Methods 0.000 description 6
- 230000035772 mutation Effects 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 239000011324 bead Substances 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 230000004907 flux Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000012224 working solution Substances 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 2
- 230000009514 concussion Effects 0.000 description 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 238000012350 deep sequencing Methods 0.000 description 2
- 239000012154 double-distilled water Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 239000002096 quantum dot Substances 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 125000005909 ethyl alcohol group Chemical group 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 101150044508 key gene Proteins 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010206 sensitivity analysis Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Barcode Name | Sample | Mapped Reads | On Taret | Mean Depth | Uniformity |
gao-4-1 | gao-4-1 | 16232 | 85.54% | 544.2 | 89.55% |
gao-4-2 | gao-4-2 | 8026 | 67.54% | 207.8 | 90.88% |
Barcode Name | Sample | Bases | ≥Q20Bases | Reads | Mean Read Length |
gao-4-1 | gao-4-1 | 2042523 | 1887663 | 16635 | 123bp |
gao-4-2 | gao-4-2 | 1042969 | 958366 | 8477 | 123bp |
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711039852.1A CN107586847B (zh) | 2017-10-30 | 2017-10-30 | 一种环形接头及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711039852.1A CN107586847B (zh) | 2017-10-30 | 2017-10-30 | 一种环形接头及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107586847A true CN107586847A (zh) | 2018-01-16 |
CN107586847B CN107586847B (zh) | 2024-06-21 |
Family
ID=61045014
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711039852.1A Active CN107586847B (zh) | 2017-10-30 | 2017-10-30 | 一种环形接头及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107586847B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111139533A (zh) * | 2019-09-27 | 2020-05-12 | 上海英基生物科技有限公司 | 稳定性增加的测序文库接头 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016058134A1 (zh) * | 2014-10-14 | 2016-04-21 | 深圳华大基因科技有限公司 | 一种接头元件和使用其构建测序文库的方法 |
CN105714383A (zh) * | 2014-12-22 | 2016-06-29 | 深圳华大基因研究院 | 一种基于分子反向探针的测序文库构建方法和试剂 |
CN106906211A (zh) * | 2017-04-13 | 2017-06-30 | 江苏为真生物医药技术股份有限公司 | 一种分子接头及其应用 |
KR20210090603A (ko) * | 2019-12-26 | 2021-07-20 | 이원다이애그노믹스(주) | 이중 가닥 핵산 분자 및 이를 이용한 dna 라이브러리 내 유리 어댑터 제거 방법 |
-
2017
- 2017-10-30 CN CN201711039852.1A patent/CN107586847B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016058134A1 (zh) * | 2014-10-14 | 2016-04-21 | 深圳华大基因科技有限公司 | 一种接头元件和使用其构建测序文库的方法 |
CN105714383A (zh) * | 2014-12-22 | 2016-06-29 | 深圳华大基因研究院 | 一种基于分子反向探针的测序文库构建方法和试剂 |
CN106906211A (zh) * | 2017-04-13 | 2017-06-30 | 江苏为真生物医药技术股份有限公司 | 一种分子接头及其应用 |
KR20210090603A (ko) * | 2019-12-26 | 2021-07-20 | 이원다이애그노믹스(주) | 이중 가닥 핵산 분자 및 이를 이용한 dna 라이브러리 내 유리 어댑터 제거 방법 |
Non-Patent Citations (2)
Title |
---|
TATYANA GORBACHEVA 等: "Improved transposon-based library preparation for the Ion Torrent platform", BIOTECHNIQUES, vol. 58, no. 4, pages 200, XP055403825, DOI: 10.2144/000114277 * |
杨琼 等: "水稻二化螟和三化螟基因组ddRADseq 文库的构建", 环境昆虫学报, vol. 38, no. 6, pages 1114 - 1120 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111139533A (zh) * | 2019-09-27 | 2020-05-12 | 上海英基生物科技有限公司 | 稳定性增加的测序文库接头 |
Also Published As
Publication number | Publication date |
---|---|
CN107586847B (zh) | 2024-06-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105821138B (zh) | 一种基于连接反应构建双茎环结构dna模板检测核酸的方法 | |
CN107541791A (zh) | 血浆游离dna甲基化检测文库的构建方法、试剂盒及应用 | |
CN110257480A (zh) | 核酸序列测序接头及其构建测序文库的方法 | |
CN108753954B (zh) | 痴呆相关基因的捕获探针组、试剂盒、文库构建方法和用途 | |
CN106835291B (zh) | Dna文库的制备方法以及试剂盒 | |
CN108624695B (zh) | 一种与甲状腺乳头状癌辅助诊断相关的循环miRNA标志物及其应用 | |
CN104946639B (zh) | 构建基因突变测序文库的引物和方法以及试剂盒 | |
CN106987640A (zh) | Pik3ca基因突变检测引物探针及其试剂盒 | |
CN106757379A (zh) | 肺癌多基因变异文库构建方法 | |
CN108998508A (zh) | 扩增子测序文库的构建方法及引物组和试剂盒 | |
CN107619867A (zh) | 用于同时检测肺癌多种基因突变类型的序列组合和探针 | |
CN107312770A (zh) | 一种用于高通量测序检测的肿瘤brca1/2基因变异文库的构建方法及其应用 | |
CN109385469A (zh) | 一种高灵敏度双链循环肿瘤dna检测方法及试剂盒 | |
CN106868124A (zh) | 一组甲基化基因及其检测方法 | |
CN106754878A (zh) | 乳腺癌易感基因变异文库构建方法 | |
WO2019149093A1 (zh) | 一种用于检测食管癌的基因标志物及其用途和检测方法 | |
CN103998625B (zh) | 用于病毒检测的方法和系统 | |
CN108624693A (zh) | miR-577在制备肾病诊断标志物中的应用 | |
CN107058579A (zh) | 肺腺癌相关的miRNA、组合物及其应用 | |
CN107604067A (zh) | 一种用于检测目的基因低频突变的引物及试剂盒 | |
CN107586847A (zh) | 一种环形接头及其应用 | |
CN109252224A (zh) | 一种环状探针和基于环状探针捕获的测序文库构建方法 | |
CN107058548A (zh) | c‑kit基因突变检测引物探针及其试剂盒 | |
CN110241211A (zh) | 诊断结直肠癌的分子组合标志物及检测试剂盒 | |
CN106757378B (zh) | 结直肠癌易感基因变异文库构建方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20231101 Address after: Gao Lou Zhen Hong Di Cun, Rui'an City, Wenzhou City, Zhejiang Province, 325200 Applicant after: Wang Conghai Address before: 200436, 2nd Floor, Wenjing Building, No.1 Shangda Road, Baoshan District, Shanghai Applicant before: SHANGHAI SHINES PHARMACEUTICALS Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20240516 Address after: Room 332, 3rd Floor, Building 2, No. 36 Zhenxing Road, Science and Technology Park, Changping District, Beijing, 100000 Applicant after: Beijing Zhongmei Technology Co.,Ltd. Country or region after: China Address before: Gao Lou Zhen Hong Di Cun, Rui'an City, Wenzhou City, Zhejiang Province, 325200 Applicant before: Wang Conghai Country or region before: China |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant |