CN107582513A

CN107582513A - A kind of postoperative biology cellulose facial mask of laser beautifying and preparation method thereof

Info

Publication number: CN107582513A
Application number: CN201710821478.4A
Authority: CN
Inventors: 李喆
Original assignee: Suzhou Lanshi Medical Mstar Technology Ltd
Current assignee: Jiaxing University
Priority date: 2016-09-14
Filing date: 2017-09-13
Publication date: 2018-01-16
Anticipated expiration: 2037-09-13
Also published as: CN107582513B

Abstract

The present invention relates to postoperative biology cellulose facial mask of a kind of laser beautifying and preparation method thereof, preparation method is：Bacterium solution is added to dispersed rear quiescent culture in culture emulsion and obtains biological cellulose membrane, after post-treated and natural component extract is compound obtains the postoperative biology cellulose facial mask of laser beautifying, bacterium solution is the system that bacterial strain disperses in water, culture emulsion is emulsion oil-in-water, wherein aqueous phase is strain cultured solution, and bacterial strain is to refer to the cellulose nano-fibrous microorganism of biosynthesis.Postoperative biology cellulose facial mask of a kind of laser beautifying of the present invention and preparation method thereof, it is simple and easy, green non-pollution, material self structure easy-regulating in preparation process, the postoperative biology cellulose facial mask retentiveness of laser beautifying made from final is high, and the submissive property of face is good, using with carrying simplicity, the malaise symptoms such as postoperative caused facial red, swollen, scorching hot, the dry or water shortage of laser beautifying can effectively be alleviated rapidly, there is fabulous promotional value.

Description

A kind of postoperative biology cellulose facial mask of laser beautifying and preparation method thereof

Technical field

The invention belongs to field of medical materials, is related to a kind of biology cellulose facial mask and preparation method thereof, particularly relates to energy Skin of face is spread on, biology cellulose facial mask postoperative for laser beautifying and preparation method thereof.

Background technology

In recent years, with the theoretical development with technology of laser medicine, laser beautifying has turned into a kind of fast, efficient, safe Beautifying technique.Laser beautifying has the incomparable superiority of traditional treatment, have bleeding is few, pain is small, operation precision is high, Time short the advantages that lacking with scar.With the popularization of laser beautifying, associated laser beautifying consumptive material is also by with wide Market.

Laser is the monochromatic light with certain penetration power, focuses on accurately, is commonly applied to during beauty treatment, main function High heat is produced in the local of tissue simultaneously in tissue, so as to reach beauty or therapeutic purposes.Laser beautifying process Middle laser can produce strong fuel factor with skin histology, the epidermis, corium or skin accessory organ of skin is caused different journeys Degree is burnt, and so as to inevitably cause tissue to dry and the phenomenon of water shortage, have impact on the protection of skin itself, moisturizing, anti- Inflammation and the physiological function such as sun-proof.For conventional non-exfoliative laser beautifying, clinical signs occur for aftertreatment position Slight red and swollen and burning heat sensation, phenomena such as even obvious oedema, blister and oozing of blood occurs in severe patient.Therefore, laser beautifying art Nursing need carries out fast and effectively cooling and moisturizing to therapentic part while accelerates wound repair afterwards.

Patent CN104474577B discloses a kind of face-beautifying dressing liquid after being used for laser operation, postoperative by dressing in laser beautifying Liquid is applied to whole face, has the function that moisturizing and accelerates wound repair.But the dressing liquid of liquid is smeared when using more not Just, and cooling-down effect is very limited.Patent CN201310141257.4 discloses a kind of laser prepared using bacteria cellulose Facial mask is repaired after iatrotechnics, has upper surface is fine and close to be gradually transitions the loose structure in lower surface downwards.The structure lacks micron Level macropore, the drugloading rate and water-holding capacity during actual use are less, it is difficult to meet the requirement of usage time.

Therefore, study a kind of moisture content is high, drugloading rate is high, it is easy to use, prepare material itself in easy and preparation process Postoperative biology cellulose facial mask of laser beautifying of structure easy-regulating and preparation method thereof is extremely important.

The content of the invention

The purpose of the present invention is the biology cellulose facial mask for overcoming laser beautifying present in prior art postoperative using not Just, drugloading rate is low, water-holding capacity is low, preparation technology is complicated and preparation process in material self structure be difficult to regulation and control the problem of, there is provided A kind of moisture content is high, drugloading rate is high, laser easy to use, preparing material self structure easy-regulating in easy and preparation process Biology cellulose facial mask and preparation method thereof after cosmetology.

In order to achieve the above object, the technical solution adopted by the present invention is：

A kind of preparation method of the postoperative biology cellulose facial mask of laser beautifying, bacterium solution is added in culture emulsion and uniformly divided Quiescent culture obtains biological cellulose membrane after dissipating, and after post-treated and natural component extract is compound obtains the postoperative life of laser beautifying Fibres vegetarian noodles film；The bacterium solution is the system that bacterial strain disperses in water, and the culture emulsion is emulsion oil-in-water, its reclaimed water It is mutually strain cultured solution, the bacterial strain is to refer to the cellulose nano-fibrous microorganism of biosynthesis.

As preferable technical scheme：

A kind of preparation method of the postoperative biology cellulose facial mask of laser beautifying as described above, is comprised the following steps that：

(1) strain cultured solution is prepared；

By different component it is well mixed after obtain strain cultured solution, each component and its content are in the strain cultured solution： 2~5wt% of glucose, 0.05~0.5wt% of peptone, 0.05~0.5wt% of yeast extract, 0.01~0.1wt% of citric acid, phosphorus Sour 0.02~0.2wt% of disodium hydrogen, 0.01~0.1wt% of potassium dihydrogen phosphate, surplus are water；

(2) culture emulsion and bacterium solution are prepared；

The culture emulsion that pH value is 5~7 will be obtained after different component emulsifying, counted in parts by weight, the culture breast Each component and its content are in liquid：50~80 parts of strain cultured solution, 5~30 parts of oil, 3~15 parts of emulsifying agent, thickening stabilizing agent 5~ 22 parts；

By the bacterial strain of refrigeration in thaw at RT, be placed in strain cultured solution, under the conditions of 28~32 DEG C shaking table culture 6~ 24h, supplement add strain cultured solution and are well mixed, and quiescent culture obtains bacterium solution in 3~5 days under the conditions of 28~32 DEG C, described The rotating speed of shaking table is 50~150r/min during shaking table culture, and the strain cultured solution for supplementing addition is the 10 of opportunistic pathogen strain nutrient solution volume ~100 times；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 28~32 DEG C quiescent culture given birth within 2~5 days The volume ratio of fibres element film, the bacterium solution and culture emulsion is 1:10~1000；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 1~4wt% is dipped to and boils guarantor Hold 0.5~2 hour, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 40~80wt% that biology cellulose face mask substrate material, which is dehydrated to water content, is then immersed in containing 10~30wt% days So in the aqueous solution of component extract, emulsion or cream, 10~30min of stewing process, packaged and sterilizing obtains laser beautifying Postoperative biology cellulose facial mask.

A kind of preparation method of the postoperative biology cellulose facial mask of laser beautifying as described above, in step (2), the oil is One or more of peanut oil, coconut oil, soybean oil or Dormant oils；

The emulsifying agent is in polysorbate, sucrose fatty ester, hydrolecithin or Crodaret More than one；

The thickening stabilizing agent is one in gelatin, sodium alginate, chitosan, Sodium Hyaluronate or sodium carboxymethylcellulose More than kind.

A kind of preparation method of the postoperative biology cellulose facial mask of laser beautifying as described above, in step (2), the bacterial strain For gluconacetobacter, produce acetobacter, acetify bacillus, Pasteur's acetobacter, grape saccharobacillus, Agrobacterium, rhizobium, sarcine, One or more of Pseudomonas cepacia, Pseudomonas cocovenenans or campylobacter jejuni；The species of bacterial strain is included but not in the present invention Be limited to this, can the cellulose nano-fibrous microorganism of biosynthesis can serve as the present invention bacterial strain.

The number of strain cell is 1.0 × 10 in unit volume in the bacterium solution³~1.0 × 10⁶Individual/mL.

A kind of preparation method of the postoperative biology cellulose facial mask of laser beautifying as described above, in step (5), the dehydration The method used drains for centrifugation, is dried in vacuo or mechanical expression；

The natural component extract refers to that radix scutellariae root extract, peony root extractive, german chamomile flower extract, hairyvein agrimony carry Take thing, papaya extractives, Saussurea involucrata extract, dried tremella extract, bletilla striata extract, Leonurus extract, Bulbus Lilii extract or silk One or more of melon extract；

The sterilizing refer to sterilized using high-energy ray irradiation, pressuresteam sterilization or electron beam sterilization.

Present invention also offers the postoperative biology cellulose facial mask of laser beautifying made from a kind of preparation method as described above, The postoperative biology cellulose facial mask of laser beautifying has double-decker, is made up of upper strata tight section and lower floor's porosity and looseness part, The aqueous solution, emulsion or the cream of the extract containing natural component are filled with the hole of double-decker；

The upper strata tight section is the nanometer fiber net being interweaved overlapped to form by cellulose nano-fibrous stratiform Network, the density of upper strata tight section is 30~50kg/m³；

Lower floor's porosity and looseness part by the cellulose nano-fibrous interconnected loose structure formed, while its For hole wall surface there is cellulose nano-fibrous network, the density of lower floor's porosity and looseness part is 10~20kg/m³；It is interconnected Porous network can ensure that bio signal, oxygen and nutriment enter material internal, improve cell propagation and migration velocity, Promotion organization vascularization and the formation of cambium；And the nano-fiber material with high porosity and specific surface area can influence The interaction of cell-material, promote mass transfer, improve the cell behaviors such as cell adhesion, propagation, differentiation and matrix expression Speed.Lower floor's porosity and looseness part of biology cellulose facial mask is interlaced biology cellulose nanofiber in the present invention The porous loose structure for having um porous and nanofiber concurrently formed, have above-mentioned porous network and nano-fiber material common Advantage, can promote cell adherence and propagation, be oriented to cell and enter material internal, accelerate the vascularization of surrounding tissue so that Dramatically speed up the reparation of skin trauma.

The upper strata tight section passes through the intramolecular and molecule in β -1,4- grape sugar chains with lower floor's porosity and looseness part Between hydrogen bond combine closely.

As preferable technical scheme：

The postoperative biology cellulose facial mask of laser beautifying as described above, the extract containing natural component in the double-decker The content of the aqueous solution, emulsion or cream is 0.1~3wt%；

The thickness of the postoperative biology cellulose facial mask of laser beautifying is 0.5~3mm, the thickness of its tight section at the middle and upper levels For 0.01~0.1mm；Normal human body skin thickness is 0.5~4mm, and different physiological site skin thickness variations are larger. Therefore, the thickness of biology cellulose facial mask is controlled to have certain necessity.The biology cellulose facial mask of the present invention can pass through tune Save incubation time, number of strains and cultivate the thickness of the accuracy controlling materials such as emulsion dosage, while change cultivating system viscosity energy The proportion structure of enough further regulation double-deckers.Upper strata tight section thickness obstructs for the cell of biology cellulose facial mask to be made Had a significant impact with, moisture-vapor transmission and mechanical property etc.；And lower floor's porosity and looseness part-structure can influence biological fiber The indexs such as water absorbing properties, cell proliferation rate and the vascularization of vegetarian noodles film.Therefore, should be according to the purposes of reality in design of material Consider the thickness of biology cellulose facial mask and upper and lower stratum proportion.

The saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 180~360%, and moisture-vapor transmission is 5500~8500gm^-2·24h^-1。

The postoperative biology cellulose facial mask of laser beautifying as described above, the cellulose nano-fibrous average diameter are 20 ~60nm.

The postoperative biology cellulose facial mask of laser beautifying as described above, the average pore size of the upper strata tight section is 3~5 μm, the average pore size of lower floor's porosity and looseness part is 20~243 μm.

Invention mechanism：

The purpose of the present invention is to prepare a kind of to have similar human skin tissue acellular matrix " upper strata is fine and close, lower floor It is loose porous " the postoperative biology cellulose facial mask of laser beautifying of double-decker.The classical culture protocols of biological cellulose membrane be by Bacterial strain is dispersed in the strain cultured solution of aqueous phase, static fermentation, obtains being interleaved with each other shape by biology cellulose nanofiber Into comparatively dense three-dimensional net structure, the present invention using emulsion oil-in-water as fermentation system, oil phase is with fine droplet Form be emulsified agent parcel be dispersed in aqueous phase (strain cultured solution), while the introducing of thickener cause aqueous phase have compared with High viscosity, in the quiescent culture stage, bacterium solution is added in culture emulsion, due to bacterium solution and the aqueous phase (bacterium in culture emulsion Strain nutrient solution) it is waterborne liquid, bacterial strain will be dispersed in the aqueous phase of culture emulsion, simultaneously as the protection of emulsifying agent And the influence of aqueous phase viscosity, bacterial strain are difficult to enter into oil phase particulate.In biology cellulose biochemistry building-up process, bacterial strain Nutriment is generated cellulose microfibril, strain cell carries by cell as biosynthesis reaction device under enzyme catalysis Microfibril bundle random motion in the aqueous phase of culture emulsion, because the steric hindrance of oil phase drop acts on, strain can only be in water Biosynthesis cellulose fibre in phase, so as to form the porous structure of biology cellulose lower floor, and in the upper table of cultivating system Face, due to surface tension and oil phase drop self gravitation effect, aqueous phase forms thin layer aqueous phase liquid level on emulsion liquid level, In this less region, the culture of bacterial strain is similar to traditional approach, therefore, upper strata cause is formd in the upper surface of cultivating system Close biology cellulose film.Using the preparation method of the present invention, because strain cell is the water that is dispersed in cultivating system In phase, therefore the postoperative biology cellulose facial mask of laser beautifying formed is overall structure, and upper strata tight section and lower floor are porous Loose part is by continuous, cellulose nano-fibrous being combined by intramolecular with intermolecular hydrogen bonding to be formed of being interleaved with each other , without obvious external force lamination, while cultivate the thickener in emulsion and can adjust culture systems viscosity, control oil phase liquid Dissolved oxygen index in the distribution and system of drop, so as to further adjust biological fiber cellulose aggregate tight section and lower floor at the middle and upper levels The structure of porosity and looseness part.

Beneficial effect：

(1) the postoperative biology cellulose facial mask preparation method of a kind of laser beautifying of the invention, simple and easy, green is without dirt Dye, finally the obtained postoperative biology cellulose facial mask retentiveness of laser beautifying is high, and the submissive property of face is good, using easy with carrying.

(2) the postoperative biology cellulose facial mask of a kind of laser beautifying of the invention, has double-decker, facial mask can be made more preferable Fit perfectly in skin surface.Upper strata tight section can reduce moisture evaporation, and lower floor's porosity and looseness part drugloading rate is big, retentiveness is high, Natural component extract more effectively can be transferred to skin with skin contact, can continue, quickly, effectively supplement skin water Divide and accelerate wound repair.

(3) the postoperative biology cellulose facial mask of a kind of laser beautifying of the invention, can effectively alleviate rapidly non-exfoliative and swash The malaise symptoms such as red, swollen, scorching hot, dry or water shortage of caused face after light cosmetology.

Brief description of the drawings

Fig. 1 is the schematic cross-section of the postoperative biology cellulose facial mask of laser beautifying of the present invention；

Fig. 2 is the cross-sectional scans electron microscope of the postoperative biology cellulose facial mask of laser beautifying of the present invention；

Fig. 3 is the cross-sectional scans electron microscope of Fig. 2 tight sections at the middle and upper levels；

Fig. 4 is the cross-sectional scans electron microscope of Tu2Zhong lower floors porosity and looseness part；

Wherein, 1- upper stratas tight section, 2- lower floors porosity and looseness part.

Embodiment

The invention will be further elucidated with reference to specific embodiments.It should be understood that these embodiments are merely to illustrate this hair Bright rather than limitation the scope of the present invention.In addition, it is to be understood that after the content of the invention lectured has been read, art technology Personnel can make various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited Fixed scope.

Embodiment 1

A kind of preparation method of the postoperative biology cellulose facial mask of laser beautifying, is comprised the following steps that：

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 2wt%, the content of peptone are 0.3wt%, and the content of yeast extract is 0.5wt%, and the content of citric acid is 0.05wt%, phosphoric acid The content of disodium hydrogen is 0.02wt%, and the content of potassium dihydrogen phosphate is 0.1wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

By strain cultured solution, 6 parts of peanut oil, 15 parts of polysorbate and 5 parts of the gelatin that parts by weight are 50 parts The culture emulsion that pH value is 5 is obtained after carrying out emulsifying；

The gluconacetobacter of refrigeration is thawed at room temperature, is placed in strain cultured solution, shaking table is trained under the conditions of 28 DEG C 7h is supported, supplement adds strain cultured solution and is well mixed, and quiescent culture obtains bacterium solution for 3 days under the conditions of 32 DEG C, and wherein shaking table is trained The rotating speed of shaking table is 100r/min when supporting, and the strain cultured solution for supplementing addition is 20 times of opportunistic pathogen strain nutrient solution volume, is obtained The number of strain cell is 1.0 × 10 in unit volume in bacterium solution³Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 28 DEG C quiescent culture obtain biology cellulose within 3 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:30；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 1wt% is dipped to and boils holding 0.5 hour, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 40wt% that the centrifugation of biology cellulose face mask substrate material, which is drained to water content, is then immersed in the radix scutellariae containing 10wt% Root extract and peony root extractive (mass ratio 1:1) in cream, stewing process 30min, packaged and use high energy is penetrated Line irradiation sterilization obtains the postoperative biology cellulose facial mask of laser beautifying.

As shown in Fig. 1~2, had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps and caused by upper strata The double-decker that compact part point 1 and lower floor's porosity and looseness part 2 are formed, it is filled with content in the hole of double-decker 0.1wt% root extract containing radix scutellariae and the cream of peony root extractive, both pass through the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain Combined closely with intermolecular hydrogen bonding.As shown in figure 3, upper strata tight section 1 is overlapped to form by cellulose nano-fibrous stratiform The Nanofiber Network being interweaved, the density measured are 40kg/m³, average pore size is 3 μm；Dredged as shown in figure 4, lower floor is porous Loose part 2 is by the cellulose nano-fibrous interconnected loose structure formed, while there is cellulose for its hole wall surface Nanofiber Network, the density measured are 20kg/m³, average pore size is 150 μm, wherein cellulose nano-fibrous average diameter For 46nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 1mm, the thickness of its tight section at the middle and upper levels For 0.02mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 200%, moisture-vapor transmission 5700g m^-2·24h^-1。

Embodiment 2

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 3wt%, the content of peptone are 0.10wt%, and the content of yeast extract is 0.05wt%, and the content of citric acid is 0.09wt%, phosphorus The content of sour disodium hydrogen is 0.05wt%, and the content of potassium dihydrogen phosphate is 0.01wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

By strain cultured solution that parts by weight are 52 parts, 5 parts of coconut oil, 3 parts of sucrose fatty ester and 22 parts Sodium alginate obtains the culture emulsion that pH value is 7 after carrying out emulsifying；

The production acetobacter of refrigeration is thawed at room temperature, is placed in strain cultured solution, the shaking table culture under the conditions of 28 DEG C 24h, supplement add strain cultured solution and are well mixed, and quiescent culture obtains bacterium solution, wherein shaking table culture in 3 days under the conditions of 32 DEG C When shaking table rotating speed be 60r/min, the strain cultured solution for supplementing addition is 30 times of opportunistic pathogen strain nutrient solution volume, obtained bacterium solution The number of strain cell is 5.5 × 10 in middle unit volume³Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 32 DEG C quiescent culture obtain biology cellulose within 2 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:10；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 2wt% is dipped to and boils holding 0.5 hour, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 45wt% that biology cellulose face mask substrate material is dried under vacuum into water content, is then immersed in the german chamomile containing 15wt% Flower extract and Herba Agrimoniae extract (mass ratio 1:2) in cream, stewing process 25min, packaged and use pressure is steamed Vapour sterilizes to obtain the postoperative biology cellulose facial mask of laser beautifying.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being carried containing Flos Chrysanthemi for 0.3wt% in the hole of double-decker The cream of thing and Herba Agrimoniae extract is taken, both are closely tied by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding Close.Upper strata tight section is the Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, is measured Density is 30kg/m³, average pore size is 3 μm；Lower floor's porosity and looseness part by it is cellulose nano-fibrous form be interconnected Loose structure, while its hole wall surface, there is cellulose nano-fibrous network, the density measured is 10.5kg/m³, it is average Aperture is 20 μm, wherein cellulose nano-fibrous average diameter is 20nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 1.5mm, the thickness of its tight section at the middle and upper levels Spend for 0.08mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 180%, and moisture-vapor transmission is 5500g·m^-2·24h^-1。

Embodiment 3

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 4wt%, the content of peptone are 0.4wt%, and the content of yeast extract is 0.15wt%, and the content of citric acid is 0.1wt%, phosphoric acid The content of disodium hydrogen is 0.02wt%, and the content of potassium dihydrogen phosphate is 0.08wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

By strain cultured solution, 9 parts of soybean oil, 5 parts of hydrolecithin and 21 parts of the shell that parts by weight are 56 parts Glycan obtains the culture emulsion that pH value is 6 after carrying out emulsifying；

The bacillus that acetifies of refrigeration is thawed at room temperature, is placed in strain cultured solution, the shaking table culture under the conditions of 29 DEG C 10h, supplement add strain cultured solution and are well mixed, and quiescent culture obtains bacterium solution, wherein shaking table culture in 3 days under the conditions of 29 DEG C When shaking table rotating speed be 50r/min, the strain cultured solution for supplementing addition is 10 times of opportunistic pathogen strain nutrient solution volume, obtained bacterium solution The number of strain cell is 1.0 × 10 in middle unit volume⁴Individual/mL.

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 28 DEG C quiescent culture obtain biology cellulose within 3 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:70；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 3wt% is dipped to and boils holding 1 Hour, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 50wt% by biology cellulose face mask substrate material mechanical expression to water content, is then immersed in the pawpaw containing 20wt% Extract, Saussurea involucrata extract and dried tremella extract (mass ratio 1:2:2) in cream, stewing process 20min is packaged and adopt The postoperative biology cellulose facial mask of laser beautifying is obtained with electron beam sterilization.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being extracted containing pawpaw for 0.5wt% in the hole of double-decker The cream of thing, Saussurea involucrata extract and dried tremella extract, both pass through the intramolecular and intermolecular hydrogen bonding in β-Isosorbide-5-Nitrae-grape sugar chain Combine closely.Upper strata tight section is the Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, The density measured is 38kg/m³, average pore size is 4 μm；Lower floor's porosity and looseness part is by the cellulose nano-fibrous phase formed Intercommunicated loose structure, while its hole wall surface, there is cellulose nano-fibrous network, the density measured is 10kg/m³, Average pore size is 80 μm, wherein cellulose nano-fibrous average diameter is 30nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 2.0mm, the thickness of its tight section at the middle and upper levels Spend for 0.02mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 220%, and moisture-vapor transmission is 5800g·m^-2·24h^-1。

Embodiment 4

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 5wt%, the content of peptone are 0.05wt%, and the content of yeast extract is 0.1wt%, and the content of citric acid is 0.08wt%, phosphorus The content of sour disodium hydrogen is 0.04wt%, and the content of potassium dihydrogen phosphate is 0.07wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

The strain cultured solution, 12 parts of Dormant oils, 7 parts of Crodaret and 17 for being 65 parts by parts by weight The Sodium Hyaluronate of part obtains the culture emulsion that pH value is 7 after carrying out emulsifying；

Pasteur's acetobacter of refrigeration is thawed at room temperature, is placed in strain cultured solution, shaking table is trained under the conditions of 30 DEG C 6h is supported, supplement adds strain cultured solution and is well mixed, and quiescent culture obtains bacterium solution for 4 days under the conditions of 28 DEG C, and wherein shaking table is trained The rotating speed of shaking table is 90r/min when supporting, and supplements the strain cultured solution of addition as 40 times of opportunistic pathogen strain nutrient solution volume, obtained bacterium The number of strain cell is 6.2 × 10 in unit volume in liquid⁵Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 30 DEG C quiescent culture obtain biology cellulose within 2 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:90；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 4wt% is dipped to and boils holding 2 Hour, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 55wt% that the centrifugation of biology cellulose face mask substrate material, which is drained to water content, is then immersed in the bletilla striata containing 25wt% Extract, Leonurus extract and Bulbus Lilii extract (mass ratio 1:1:1) in cream, stewing process 15min, it is packaged and The postoperative biology cellulose facial mask of laser beautifying is obtained using electron beam sterilization.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being extracted containing the bletilla striata for 0.8wt% in the hole of double-decker The cream of thing, Leonurus extract and Bulbus Lilii extract, both pass through the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain and intermolecular hydrogen Key is combined closely.Upper strata tight section is the nanometer fiber net being interweaved overlapped to form by cellulose nano-fibrous stratiform Network, the density measured are 40kg/m³, average pore size is 4.2 μm；Lower floor's porosity and looseness part is made up of cellulose nano-fibrous Interconnected loose structure, while its hole wall surface, there is cellulose nano-fibrous network, the density measured is 11kg/ m³, average pore size is 20 μm, wherein cellulose nano-fibrous average diameter is 28nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 0.5mm, the thickness of its tight section at the middle and upper levels Spend for 0.06mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 270%, and moisture-vapor transmission is 8500g·m^-2·24h^-1。

Embodiment 5

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 2.5wt%, the content of peptone is 0.25wt%, and the content of yeast extract is 0.2wt%, and the content of citric acid is 0.07wt%, The content of disodium hydrogen phosphate is 0.08wt%, and the content of potassium dihydrogen phosphate is 0.06wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

Mixture (mass ratio 1 by parts by weight for 68 parts of strain cultured solution, 17 parts of peanut oil and soybean oil: 1), the mixture of 8 parts of polysorbate and sucrose fatty ester (mass ratio 2:1) and 22 parts of sodium carboxymethylcellulose enters The culture emulsion that pH value is 6 is obtained after row emulsifying；

The grape saccharobacillus of refrigeration is thawed at room temperature, is placed in strain cultured solution, shaking table is trained under the conditions of 29 DEG C 8h is supported, supplement adds strain cultured solution and is well mixed, and quiescent culture obtains bacterium solution for 4 days under the conditions of 30 DEG C, and wherein shaking table is trained The rotating speed of shaking table is 90r/min when supporting, and supplements the strain cultured solution of addition as 20 times of opportunistic pathogen strain nutrient solution volume, obtained bacterium The number of strain cell is 1.0 × 10 in unit volume in liquid⁶Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 31 DEG C quiescent culture obtain biology cellulose within 5 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:700；

(4) post-process；

(5) it is compound；

It is 60wt% that biology cellulose face mask substrate material is dried under vacuum into water content, is then immersed in the bletilla striata containing 30wt% Extract, Leonurus extract, Bulbus Lilii extract and Fructus Luffae extract (mass ratio 1:1:2:2) in emulsion, stewing process 10min, packaged and use electron beam sterilization obtain the postoperative biology cellulose facial mask of laser beautifying.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, in the hole of double-decker filled with content be 1wt% containing bletilla striata extract, The emulsion of Leonurus extract, Bulbus Lilii extract and Fructus Luffae extract, both by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with Intermolecular hydrogen bonding is combined closely.Upper strata tight section is the nanometer being interweaved overlapped to form by cellulose nano-fibrous stratiform Network of fibers, the density measured are 31kg/m³, average pore size is 3.5 μm；Lower floor's porosity and looseness part is by cellulose Nanowire The interconnected loose structure of composition is tieed up, while its hole wall surface is there is cellulose nano-fibrous network, the density measured For 13.5kg/m³, average pore size is 185 μm, wherein cellulose nano-fibrous average diameter is 60nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 1.7mm, the thickness of its tight section at the middle and upper levels Spend for 0.1mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 310%, moisture-vapor transmission 5500g m^-2·24h^-1。

Embodiment 6

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 3.5wt%, the content of peptone is 0.45wt%, and the content of yeast extract is 0.25wt%, and the content of citric acid is 0.06wt%, The content of disodium hydrogen phosphate is 0.09wt%, and the content of potassium dihydrogen phosphate is 0.04wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

Mixture (mass ratio 2 by parts by weight for 50 parts of strain cultured solution, 20 parts of coconut oil and Dormant oils:1)、 12 parts of hydrolecithin and the mixture (mass ratio 1 of Crodaret:And 13 parts of gelatin and marine alga 1) Mixture (the mass ratio 2 of sour sodium:1) the culture emulsion that pH value is 6 is obtained after carrying out emulsifying；

The Agrobacterium of refrigeration is thawed at room temperature, is placed in strain cultured solution, the shaking table culture under the conditions of 31 DEG C 11h, supplement add strain cultured solution and are well mixed, and quiescent culture obtains bacterium solution, wherein shaking table culture in 3 days under the conditions of 30 DEG C When shaking table rotating speed be 110r/min, the strain cultured solution for supplementing addition is 60 times of opportunistic pathogen strain nutrient solution volume, obtained bacterium The number of strain cell is 1.6 × 10 in unit volume in liquid⁴Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 29 DEG C quiescent culture obtain biology cellulose within 2 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:100；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 1wt% is dipped to and boils holding 1.5 hours, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 65wt% by biology cellulose face mask substrate material mechanical expression to water content, is then immersed in the radix scutellariae containing 28wt% Root extract, peony root extractive, german chamomile flower extract and Herba Agrimoniae extract (mass ratio 1:1:1:2) it is quiet in emulsion Processing 12min is put, packaged and use high-energy ray irradiation sterilizes to obtain the postoperative biology cellulose facial mask of laser beautifying.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being carried containing scutellariae,radix for 1.2wt% in the hole of double-decker The emulsion of thing, peony root extractive, german chamomile flower extract and Herba Agrimoniae extract is taken, both pass through in β-Isosorbide-5-Nitrae-grape sugar chain Intramolecular is combined closely with intermolecular hydrogen bonding.Upper strata tight section is the mutual friendship overlapped to form by cellulose nano-fibrous stratiform The Nanofiber Network knitted, the density measured are 50kg/m³, average pore size is 3.6 μm；Lower floor's porosity and looseness part is by fiber The interconnected loose structure of plain nanofiber composition, while its hole wall surface is surveyed there is cellulose nano-fibrous network The density obtained is 10kg/m³, average pore size is 125 μm, wherein cellulose nano-fibrous average diameter is 49nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 1.6mm, the thickness of its tight section at the middle and upper levels Spend for 0.1mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 270%, moisture-vapor transmission 8000g m^-2·24h^-1。

Embodiment 7

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 4.5wt%, the content of peptone is 0.35wt%, and the content of yeast extract is 0.35wt%, and the content of citric acid is 0.05wt%, The content of disodium hydrogen phosphate is 0.11wt%, and the content of potassium dihydrogen phosphate is 0.05wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

Mixture (mass ratio by parts by weight for 60 parts of strain cultured solution, 23 parts of peanut oil, coconut oil and Dormant oils For 1:2:1), the mixture of 15 parts of polysorbate and Crodaret (mass ratio 1:And 12 parts of shell 1) The mixture of glycan and Sodium Hyaluronate (mass ratio 1:1) the culture emulsion that pH value is 7 is obtained after carrying out emulsifying；

The rhizobium of refrigeration are thawed at room temperature, are placed in strain cultured solution, the shaking table culture under the conditions of 32 DEG C 13h, supplement add strain cultured solution and are well mixed, and quiescent culture obtains bacterium solution, wherein shaking table culture in 5 days under the conditions of 32 DEG C When shaking table rotating speed be 120r/min, the strain cultured solution for supplementing addition is 80 times of opportunistic pathogen strain nutrient solution volume, obtained bacterium The number of strain cell is 8.0 × 10 in unit volume in liquid⁵Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 31 DEG C quiescent culture obtain biology cellulose within 5 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:200；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 3wt% is dipped to and boils holding 1.2 hours, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 70wt% that the centrifugation of biology cellulose face mask substrate material, which is drained to water content, is then immersed in the radix scutellariae containing 10wt% In the aqueous solution of root extract, stewing process 10min is packaged and sterilize to obtain laser beautifying using high-energy ray irradiation postoperative Biology cellulose facial mask.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being carried containing scutellariae,radix for 1.4wt% in the hole of double-decker The aqueous solution of thing is taken, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata compact part It is the Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform to divide, and the density measured is 49kg/m³, Average pore size is 3 μm；Lower floor's porosity and looseness part is by the cellulose nano-fibrous interconnected loose structure formed, simultaneously For its hole wall surface there is cellulose nano-fibrous network, the density measured is 18kg/m³, average pore size is 20 μm, wherein fine The average diameter for tieing up plain nanofiber is 40nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 3mm, the thickness of its tight section at the middle and upper levels For 0.05mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 220%, moisture-vapor transmission 5900g m^-2·24h^-1。

Embodiment 8

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 5wt%, the content of peptone are 0.15wt%, and the content of yeast extract is 0.3wt%, and the content of citric acid is 0.03wt%, phosphorus The content of sour disodium hydrogen is 0.12wt%, and the content of potassium dihydrogen phosphate is 0.1wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

Mixture (mass ratio by parts by weight for 74 parts of strain cultured solution, 26 parts of coconut oil, soybean oil and Dormant oils For 2：1：1), the mixture (mass ratio 2 of 4 parts of polysorbate, sucrose fatty ester and hydrolecithin:1:And 10 2) Part sodium alginate, the mixture (mass ratio 1 of chitosan and Sodium Hyaluronate:1:2) pH value is obtained after carrying out emulsifying For 5 culture emulsion；

The sarcine of refrigeration is thawed at room temperature, is placed in strain cultured solution, the shaking table culture under the conditions of 32 DEG C 6h, supplement add strain cultured solution and are well mixed, and quiescent culture obtains bacterium solution, wherein shaking table culture in 3 days under the conditions of 28 DEG C When shaking table rotating speed be 50r/min, the strain cultured solution for supplementing addition is 10 times of opportunistic pathogen strain nutrient solution volume, obtained bacterium solution The number of strain cell is 3.0 × 10 in middle unit volume⁴Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 32 DEG C quiescent culture obtain biology cellulose within 3 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:300；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 4wt% is dipped to and boils holding 1.7 hours, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 75wt% that biology cellulose face mask substrate material is dried under vacuum into water content, is then immersed in the tree peony containing 17wt% The emulsion of root extract, stewing process 14min, packaged and use pressuresteam sterilization obtain the postoperative biological fiber of laser beautifying Vegetarian noodles film.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being carried containing tree peony root for 1.8wt% in the hole of double-decker The emulsion of thing is taken, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata tight section It is the Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, the density measured is 31kg/m³, put down Equal aperture is 3.3 μm；Lower floor's porosity and looseness part is by the cellulose nano-fibrous interconnected loose structure formed, simultaneously For its hole wall surface there is cellulose nano-fibrous network, the density measured is 18kg/m³, average pore size is 65 μm, wherein fine The average diameter for tieing up plain nanofiber is 35nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 0.9mm, the thickness of its tight section at the middle and upper levels Spend for 0.01mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 200%, and moisture-vapor transmission is 7000g·m^-2·24h^-1。

Embodiment 9

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 2wt%, the content of peptone are 0.2wt%, and the content of yeast extract is 0.4wt%, and the content of citric acid is 0.02wt%, phosphoric acid The content of disodium hydrogen is 0.14wt%, and the content of potassium dihydrogen phosphate is 0.03wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

Mixture by parts by weight for 72 parts of strain cultured solution, 30 parts of peanut oil, coconut oil, soybean oil and Dormant oils (mass ratio 1:2:2:1), the mixture (matter of 8 parts of sucrose fatty ester, hydrolecithin and Crodaret Amount is than being 1:1:And 8 parts of gelatin, sodium alginate, the mixture (mass ratio of Sodium Hyaluronate and sodium carboxymethylcellulose 2) For 1:2:2:1) the culture emulsion that pH value is 6 is obtained after carrying out emulsifying；

The Pseudomonas cepacia of refrigeration is thawed at room temperature, is placed in strain cultured solution, the shaking table under the conditions of 29 DEG C 15h is cultivated, supplement adds strain cultured solution and is well mixed, and quiescent culture obtains bacterium solution, wherein shaking table in 4 days under the conditions of 31 DEG C The rotating speed of shaking table is 130r/min during culture, and the strain cultured solution for supplementing addition is 85 times of opportunistic pathogen strain nutrient solution volume, is obtained Bacterium solution in unit volume the number of strain cell be 1.0 × 10³Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 28 DEG C quiescent culture obtain biology cellulose within 4 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:600；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 3wt% is dipped to and boils holding 0.6 hour, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 80wt% by biology cellulose face mask substrate material mechanical expression to water content, is then immersed in the german chamomile containing 13wt% In the aqueous solution of flower extract, stewing process 18min is packaged and sterilize to obtain laser beautifying using high-energy ray irradiation postoperative Biology cellulose facial mask.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being carried containing Flos Chrysanthemi for 2.1wt% in the hole of double-decker The aqueous solution of thing is taken, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata compact part It is the Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform to divide, and the density measured is 37kg/m³, Average pore size is 4 μm；Lower floor's porosity and looseness part is by the cellulose nano-fibrous interconnected loose structure formed, simultaneously For its hole wall surface there is cellulose nano-fibrous network, the density measured is 12kg/m³, average pore size is 200 μm, wherein fine The average diameter for tieing up plain nanofiber is 20nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 1.5mm, the thickness of its tight section at the middle and upper levels Spend for 0.05mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 190%, and moisture-vapor transmission is 8500g·m^-2·24h^-1。

Embodiment 10

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 3wt%, the content of peptone are 0.10wt%, and the content of yeast extract is 0.45wt%, and the content of citric acid is 0.01wt%, phosphorus The content of sour disodium hydrogen is 0.15wt%, and the content of potassium dihydrogen phosphate is 0.02wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

Mixture (mass ratio 1 by parts by weight for 80 parts of strain cultured solution, 5 parts of peanut oil and Dormant oils:1)、3 Part polysorbate, the mixture (mass ratio 1 of hydrolecithin and Crodaret:2:And 6 parts of sea 2) Mosanom, chitosan, the mixture (mass ratio 1 of Sodium Hyaluronate and sodium carboxymethylcellulose:1:2:1) emulsifying is carried out The culture emulsion that pH value is 7 is obtained afterwards；

The Pseudomonas cocovenenans of refrigeration are thawed at room temperature, are placed in strain cultured solution, the shaking table under the conditions of 30 DEG C 22h is cultivated, supplement adds strain cultured solution and is well mixed, and quiescent culture obtains bacterium solution, wherein shaking table in 5 days under the conditions of 28 DEG C The rotating speed of shaking table is 140r/min during culture, and the strain cultured solution for supplementing addition is 90 times of opportunistic pathogen strain nutrient solution volume, is obtained Bacterium solution in unit volume the number of strain cell be 4.8 × 10³Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 29 DEG C quiescent culture obtain biology cellulose within 5 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:800；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 1wt% is dipped to and boils holding 0.8 hour, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 80wt% that the centrifugation of biology cellulose face mask substrate material, which is drained to water content, is then immersed in the red crowned crane containing 10wt% In the emulsion of careless extract, stewing process 21min, packaged and use electron beam sterilization obtains the postoperative biological fiber of laser beautifying Vegetarian noodles film.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being extracted containing hairyvein agrimony for 2wt% in the hole of double-decker The emulsion of thing, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata tight section is The Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, the density measured are 42kg/m³, it is average Aperture is 4.9 μm；Lower floor's porosity and looseness part by the cellulose nano-fibrous interconnected loose structure formed, while its For hole wall surface there is cellulose nano-fibrous network, the density measured is 17.3kg/m³, average pore size is 190 μm, wherein fine The average diameter for tieing up plain nanofiber is 38nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 0.5mm, the thickness of its tight section at the middle and upper levels Spend for 0.1mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 360%, moisture-vapor transmission 6400g m^-2·24h^-1。

Embodiment 11

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 3.8wt%, the content of peptone is 0.05wt%, and the content of yeast extract is 0.25wt%, and the content of citric acid is 0.01wt% , the content of disodium hydrogen phosphate is 0.2wt%, and the content of potassium dihydrogen phosphate is 0.01wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

Mixture (mass ratio by parts by weight for 56 parts of strain cultured solution, 15 parts of peanut oil, coconut oil and Dormant oils For 1:1:2), the mixture of 10 parts of polysorbate, sucrose fatty ester, hydrolecithin and Crodaret (mass ratio 1:1:2:1) and 5 parts of gelatin, sodium alginate, chitosan, Sodium Hyaluronate and sodium carboxymethylcellulose it is mixed Compound (mass ratio 1:1:2:2:1) the culture emulsion that pH value is 5 is obtained after carrying out emulsifying；

The campylobacter jejuni of refrigeration is thawed at room temperature, is placed in strain cultured solution, shaking table is trained under the conditions of 29 DEG C 24h is supported, supplement adds strain cultured solution and is well mixed, and quiescent culture obtains bacterium solution for 4 days under the conditions of 31 DEG C, and wherein shaking table is trained The rotating speed of shaking table is 150r/min when supporting, and the strain cultured solution for supplementing addition is 100 times of opportunistic pathogen strain nutrient solution volume, is obtained The number of strain cell is 1.0 × 10 in unit volume in bacterium solution⁴Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 28 DEG C quiescent culture obtain biology cellulose within 4 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:1000；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 4wt% is dipped to and boils holding 0.9 hour, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 77wt% that biology cellulose face mask substrate material is dried under vacuum into water content, is then immersed in the pawpaw containing 11wt% In the aqueous solution of extract, stewing process 15min, packaged and use high-energy ray irradiation sterilizes to obtain the postoperative life of laser beautifying Fibres vegetarian noodles film.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is that 3wt% contains papaya extractives in the hole of double-decker The aqueous solution, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata tight section is The Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, the density measured are 50kg/m³, it is average Aperture is 3 μm；Lower floor's porosity and looseness part is by the cellulose nano-fibrous interconnected loose structure formed, while its hole For wall surface there is cellulose nano-fibrous network, the density measured is 10kg/m³, average pore size is 243 μm, wherein cellulose The average diameter of nanofiber is 35nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 2.2mm, the thickness of its tight section at the middle and upper levels Spend for 0.03mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 280%, and moisture-vapor transmission is 8500g·m^-2·24h^-1。

Embodiment 12

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 2wt%, the content of peptone are 0.5wt%, and the content of yeast extract is 0.5wt%, and the content of citric acid is 0.05wt%, phosphoric acid The content of disodium hydrogen is 0.02wt%, and the content of potassium dihydrogen phosphate is 0.1wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

Mixture (mass ratio 1 by parts by weight for 50 parts of strain cultured solution, 6 parts of coconut oil and Dormant oils:1)、 15 parts of polysorbate and the mixture of 5 parts of chitosan and Sodium Hyaluronate (mass ratio 1:2) after carrying out emulsifying Obtain the culture emulsion that pH value is 6；

By the gluconacetobacter of refrigeration and onion vacation unit cell (mass ratio 1:2) thaw at room temperature, be placed in bacterial strain training In nutrient solution, the shaking table culture 24h under the conditions of 28 DEG C, supplement adds strain cultured solution and is well mixed, and is stood under the conditions of 32 DEG C It is 100r/min to cultivate 3 days rotating speeds for obtaining shaking table during bacterium solution, wherein shaking table culture, and the strain cultured solution for supplementing addition is opportunistic pathogen Strain 30 times of nutrient solution volume, the number of strain cell is 1.0 × 10 in unit volume in obtained bacterium solution³Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 28 DEG C quiescent culture obtain biology cellulose within 3 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:80；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 2wt% is dipped to and boils holding 2 Hour, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 64wt% by biology cellulose face mask substrate material mechanical expression to water content, is then immersed in the saussurea involucrata containing 29wt% In the aqueous solution of extract, stewing process 10min, packaged and use electron beam sterilization obtains the postoperative biological fiber of laser beautifying Vegetarian noodles film.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being extracted containing saussurea involucrata for 2.2wt% in the hole of double-decker The aqueous solution of thing, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata tight section The Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, the density measured are 45kg/m³, it is average Aperture is 4.5 μm；Lower floor's porosity and looseness part by the cellulose nano-fibrous interconnected loose structure formed, while its For hole wall surface there is cellulose nano-fibrous network, the density measured is 19.6kg/m³, average pore size is 28 μm, wherein fine The average diameter for tieing up plain nanofiber is 28nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 3mm, the thickness of its tight section at the middle and upper levels For 0.06mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 210%, moisture-vapor transmission 6850g m^-2·24h^-1。

Embodiment 13

(1) strain cultured solution is prepared；

(2) culture emulsion and bacterium solution are prepared；

By strain cultured solution, 7 parts of peanut oil, 15 parts of sucrose fatty ester and the hydrogenation lecithin that parts by weight are 60 parts Mixture (the mass ratio 2 of fat:2) and after 20 parts of sodium alginate progress emulsifying the culture emulsion that pH value is 5 is obtained；

Refrigeration is acetified into bacillus and Pasteur's acetobacter (mass ratio 2:1) thaw at room temperature, be placed in strain culturing In liquid, the shaking table culture 24h under the conditions of 29 DEG C, supplement adds strain cultured solution and is well mixed, and training is stood under the conditions of 32 DEG C It is 100r/min to support 3 days rotating speeds for obtaining shaking table during bacterium solution, wherein shaking table culture, and the strain cultured solution for supplementing addition is opportunistic pathogen strain 20 times of nutrient solution volume, the number of strain cell is 5.6 × 10 in unit volume in obtained bacterium solution³Individual/mL；

(3) quiescent culture；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 4wt% is dipped to and boils holding 1.1 hours, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 56wt% that the centrifugation of biology cellulose face mask substrate material, which is drained to water content, is then immersed in the white fungus containing 14wt% In the aqueous solution of extract, stewing process 30min, it is fine that packaged and use pressuresteam sterilization obtains the postoperative biology of laser beautifying Tie up vegetarian noodles film.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being extracted containing white fungus for 2.4wt% in the hole of double-decker The aqueous solution of thing, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata tight section It is the Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, the density measured is 46kg/m³, put down Equal aperture is 3.3 μm；Lower floor's porosity and looseness part is by the cellulose nano-fibrous interconnected loose structure formed, simultaneously For its hole wall surface there is cellulose nano-fibrous network, the density measured is 20kg/m³, average pore size is 37 μm, wherein fine The average diameter for tieing up plain nanofiber is 60nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 2mm, the thickness of its tight section at the middle and upper levels For 0.01mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 180%, moisture-vapor transmission 6200g m^-2·24h^-1。

Embodiment 14

(1) strain cultured solution is prepared；

(2) culture emulsion and bacterium solution are prepared；

By parts by weight be 75 parts strain cultured solution, 10 parts of soybean oil, 15 parts of Crodaret with And 5 parts of sodium alginate, the mixture (mass ratio 1 of chitosan and Sodium Hyaluronate:1:1) pH is obtained after carrying out emulsifying It is worth the culture emulsion for 7；

By Pasteur's acetobacter of refrigeration, grape saccharobacillus and Agrobacterium (mass ratio 1:1:2) thaw at room temperature, juxtaposition In strain cultured solution, the shaking table culture 24h under the conditions of 28 DEG C, supplement adds strain cultured solution and is well mixed, in 32 DEG C of bars 4 days rotating speeds for obtaining shaking table during bacterium solution, wherein shaking table culture of quiescent culture are 100r/min under part, supplement the strain culturing of addition Liquid is 20 times of opportunistic pathogen strain nutrient solution volume, and the number of strain cell is 3.5 × 10 in unit volume in obtained bacterium solution⁵Individual/ mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 28 DEG C quiescent culture obtain biology cellulose within 3 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:300；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 3wt% is dipped to and boils holding 1.5 hours, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 48wt% that biology cellulose face mask substrate material is dried under vacuum into water content, is then immersed in the bletilla striata containing 15wt% In the emulsion of extract, stewing process 30min, packaged and use pressuresteam sterilization obtains the postoperative biological fiber of laser beautifying Vegetarian noodles film.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being extracted containing the bletilla striata for 2.5wt% in the hole of double-decker The emulsion of thing, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata tight section is The Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, the density measured are 38kg/m³, it is average Aperture is 3 μm；Lower floor's porosity and looseness part is by the cellulose nano-fibrous interconnected loose structure formed, while its hole For wall surface there is cellulose nano-fibrous network, the density measured is 12.8kg/m³, average pore size is 180 μm, wherein fiber The average diameter of plain nanofiber is 55nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 0.7mm, the thickness of its tight section at the middle and upper levels Spend for 0.1mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 200%, moisture-vapor transmission 6500g m^-2·24h^-1。

Embodiment 15

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 5wt%, the content of peptone are 0.3wt%, and the content of yeast extract is 0.5wt%, and the content of citric acid is 0.05wt%, phosphoric acid The content of disodium hydrogen is 0.02wt%, and the content of potassium dihydrogen phosphate is 0.1wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

Mixture (mass ratio 2 by parts by weight for 75 parts of strain cultured solution, 20 parts of coconut oil and soybean oil: 2), 15 parts of polysorbate and 22 parts of gelatin obtain the culture emulsion that pH value is 5 after carrying out emulsifying；

By gluconacetobacter, Pseudomonas cocovenenans and the campylobacter jejuni (mass ratio 1 of refrigeration:1:1) solve at room temperature To freeze, be placed in strain cultured solution, the shaking table culture 24h under the conditions of 28 DEG C, supplement adds strain cultured solution and is well mixed, 3 days rotating speeds for obtaining shaking table during bacterium solution, wherein shaking table culture of quiescent culture are 100r/min under the conditions of 32 DEG C, supplement the bacterium of addition Strain nutrient solution is 20 times of opportunistic pathogen strain nutrient solution volume, in obtained bacterium solution in unit volume the number of strain cell for 1.0 × 10⁶Individual/mL；

(3) quiescent culture；

(4) post-process；

(5) it is compound；

It is 80wt% by biology cellulose face mask substrate material mechanical expression to water content, it is female is then immersed in the benefit containing 10wt% In the aqueous solution of careless extract, stewing process 10min, it is fine that packaged and use electron beam sterilization obtains the postoperative biology of laser beautifying Tie up vegetarian noodles film.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being carried containing motherwort for 0.8wt% in the hole of double-decker The aqueous solution of thing is taken, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata compact part It is the Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform to divide, and the density measured is 50kg/m³, Average pore size is 3.1 μm；Lower floor's porosity and looseness part is by the cellulose nano-fibrous interconnected loose structure formed, together When its hole wall surface there is cellulose nano-fibrous network, the density measured is 17.7kg/m³, average pore size is 80 μm, its In cellulose nano-fibrous average diameter be 46nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 2.3mm, the thickness of its tight section at the middle and upper levels Spend for 0.05mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 180%, and moisture-vapor transmission is 7700g·m^-2·24h^-1。

Embodiment 16

(1) strain cultured solution is prepared；

(2) culture emulsion and bacterium solution are prepared；

It is 55 parts of strain cultured solution, 6 parts of Dormant oils, 3 parts of polysorbate, sucrose fatty ester, hydrogen by parts by weight Change the mixture (mass ratio 2 of lecithin and Crodaret:1:1:2) and 5 parts chitosan carry out homogeneous The culture emulsion that pH value is 6 is obtained after emulsification；

By the production acetobacter of refrigeration, acetify bacillus, Pasteur's acetobacter and grape saccharobacillus (mass ratio 1:1:2:1) in room Temperature is lower to thaw, and is placed in strain cultured solution, the shaking table culture 24h under the conditions of 28 DEG C, supplement adds strain cultured solution and mixed Uniformly, when quiescent culture under the conditions of 32 DEG C obtains bacterium solution, wherein shaking table culture in 3 days, the rotating speed of shaking table is 100r/min, supplement The strain cultured solution of addition is 20 times of opportunistic pathogen strain nutrient solution volume, in obtained bacterium solution in unit volume strain cell number For 2.0 × 10³Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 28 DEG C quiescent culture obtain biology cellulose within 5 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:120；

(4) post-process；

(5) it is compound；

It is 80wt% that the centrifugation of biology cellulose face mask substrate material, which is drained to water content, is then immersed in the lily containing 10wt% In the aqueous solution of extract, stewing process 22min, packaged and use high-energy ray irradiation sterilizes to obtain the postoperative life of laser beautifying Fibres vegetarian noodles film.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is that 3wt% contains Bulbus Lilii extract in the hole of double-decker The aqueous solution, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata tight section is The Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, the density measured are 39kg/m³, it is average Aperture is 5 μm；Lower floor's porosity and looseness part is by the cellulose nano-fibrous interconnected loose structure formed, while its hole For wall surface there is cellulose nano-fibrous network, the density measured is 14kg/m³, average pore size is 143 μm, wherein cellulose The average diameter of nanofiber is 20nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 3mm, the thickness of its tight section at the middle and upper levels For 0.01mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 300%, moisture-vapor transmission 8300g m^-2·24h^-1。

Embodiment 17

(1) strain cultured solution is prepared；

Strain cultured solution is obtained after different component is well mixed, wherein the content of glucose is in strain cultured solution 3wt%, the content of peptone are 0.3wt%, and the content of yeast extract is 0.5wt%, and the content of citric acid is 0.05wt%, phosphoric acid The content of disodium hydrogen is 0.02wt%, and the content of potassium dihydrogen phosphate is 0.1wt%, and surplus is water；

(2) culture emulsion and bacterium solution are prepared；

By strain cultured solution that parts by weight are 58 parts, 6 parts of peanut oil, 15 parts of hydrolecithin and 5 parts it is saturating Mixture (the mass ratio 2 of bright matter acid sodium and sodium carboxymethylcellulose:1) culture that pH value is 5 is obtained after carrying out emulsifying Emulsion；

By the Agrobacterium of refrigeration, rhizobium, sarcine and Pseudomonas cepacia (mass ratio 2:1:2:1) at room temperature To thaw, be placed in strain cultured solution, the shaking table culture 24h under the conditions of 28 DEG C, supplement adds strain cultured solution and is well mixed, When quiescent culture under the conditions of 32 DEG C obtains bacterium solution, wherein shaking table culture in 3 days, the rotating speed of shaking table is 100r/min, supplements addition Strain cultured solution is 20 times of opportunistic pathogen strain nutrient solution volume, and the number of strain cell is 3.8 in unit volume in obtained bacterium solution ×10⁵Individual/mL；

(3) quiescent culture；

By bacterium solution be added to culture emulsion in it is dispersed after under the conditions of 28 DEG C quiescent culture obtain biology cellulose within 3 days The volume ratio of film, wherein bacterium solution and culture emulsion is 1:500；

(4) post-process；

(5) it is compound；

It is 50wt% by biology cellulose face mask substrate material mechanical expression to water content, is then immersed in the sponge gourd containing 20wt% In cream in extract, stewing process 19min, it is fine that packaged and use pressuresteam sterilization obtains the postoperative biology of laser beautifying Tie up vegetarian noodles film.

Had using the postoperative biology cellulose facial mask of laser beautifying made from above-mentioned steps by upper strata tight section and lower floor The double-decker that porosity and looseness part is formed, filled with content it is being extracted containing sponge gourd for 0.5wt% in the hole of double-decker Cream in thing, both are combined closely by the intramolecular in β-Isosorbide-5-Nitrae-grape sugar chain with intermolecular hydrogen bonding.Upper strata tight section It is the Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, the density measured is 40kg/m³, put down Equal aperture is 3.5 μm；Lower floor's porosity and looseness part is by the cellulose nano-fibrous interconnected loose structure formed, simultaneously For its hole wall surface there is cellulose nano-fibrous network, the density measured is 11.8kg/m³, average pore size is 200 μm, wherein Cellulose nano-fibrous average diameter is 56nm.

The thickness of the postoperative biology cellulose facial mask of resulting laser beautifying is 0.5mm, the thickness of its tight section at the middle and upper levels Spend for 0.02mm, the saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 280%, and moisture-vapor transmission is 7950g·m^-2·24h^-1。

Claims

1. a kind of preparation method of the postoperative biology cellulose facial mask of laser beautifying, it is characterized in that：Bacterium solution is added to culture emulsion In it is dispersed after quiescent culture obtain biological cellulose membrane, it is post-treated after compound to obtain laser beautiful with natural component extract Hold postoperative biology cellulose facial mask；The bacterium solution is the system that bacterial strain disperses in water, and the culture emulsion is oil-in-water type breast Liquid, wherein aqueous phase are strain cultured solution, and the bacterial strain is to refer to the cellulose nano-fibrous microorganism of biosynthesis.

A kind of 2. preparation method of the postoperative biology cellulose facial mask of laser beautifying according to claim 1, it is characterised in that Comprise the following steps that：

(1) strain cultured solution is prepared；

By different component it is well mixed after obtain strain cultured solution, each component and its content are in the strain cultured solution：Grape Sugar 2~5wt%, 0.05~0.5wt% of peptone, 0.05~0.5wt% of yeast extract, 0.01~0.1wt% of citric acid, phosphoric acid hydrogen 0.02~0.2wt% of disodium, 0.01~0.1wt% of potassium dihydrogen phosphate, surplus are water；

(2) culture emulsion and bacterium solution are prepared；

The culture emulsion that pH value is 5~7 will be obtained after different component emulsifying, counted in parts by weight, in the culture emulsion Each component and its content are：50~80 parts of strain cultured solution, 5~30 parts of oil, 3~15 parts of emulsifying agent, thickening stabilizing agent 5~22 Part；

By the bacterial strain of refrigeration in thaw at RT, it is placed in strain cultured solution, 6~24h of shaking table culture under the conditions of 28~32 DEG C, Supplement adds strain cultured solution and is well mixed, and quiescent culture obtains bacterium solution, the shaking table for 3~5 days under the conditions of 28~32 DEG C The rotating speed of shaking table is 50~150r/min during culture, supplement the strain cultured solution of addition for opportunistic pathogen strain nutrient solution volume 10~ 100 times；

(3) quiescent culture；

By bacterium solution be added in culture emulsion it is dispersed after under the conditions of 28~32 DEG C quiescent culture obtain within 2~5 days biological fibre Plain film is tieed up, the bacterium solution is 1 with the volume ratio for cultivating emulsion:10~1000；

(4) post-process；

Biological cellulose membrane is cut into facial mask shape, the sodium hydroxide solution that concentration is 1~4wt% is dipped to and boils holding 0.5~2 hour, cleaned with pure water to pH value and obtain biology cellulose face mask substrate material for 7.0；

(5) it is compound；

It is 40~80wt% that biology cellulose face mask substrate material, which is dehydrated to water content, be then immersed in containing 10~30wt% naturally into Divide in the aqueous solution, emulsion or the cream of extract, 10~30min of stewing process, it is postoperative that packaged and sterilizing obtains laser beautifying Biology cellulose facial mask.

A kind of 3. preparation method of the postoperative biology cellulose facial mask of laser beautifying according to claim 2, it is characterised in that In step (2), the oil is one or more of peanut oil, coconut oil, soybean oil or Dormant oils；

The emulsifying agent is one kind in polysorbate, sucrose fatty ester, hydrolecithin or Crodaret More than；

The thickening stabilizing agent be in gelatin, sodium alginate, chitosan, Sodium Hyaluronate or sodium carboxymethylcellulose it is a kind of with On.

A kind of 4. preparation method of the postoperative biology cellulose facial mask of laser beautifying according to claim 2, it is characterised in that In step (2), the bacterial strain is gluconacetobacter, produces acetobacter, acetifies bacillus, Pasteur's acetobacter, grape saccharobacillus, agriculture bar One or more of bacterium, rhizobium, sarcine, Pseudomonas cepacia, Pseudomonas cocovenenans or campylobacter jejuni；

A kind of 5. preparation method of the postoperative biology cellulose facial mask of laser beautifying according to claim 2, it is characterised in that In step (5), the method that uses of being dehydrated is drained for centrifugation, is dried in vacuo or mechanical expression；

The natural component extract refers to radix scutellariae root extract, peony root extractive, german chamomile flower extract, hairyvein agrimony extraction Thing, papaya extractives, Saussurea involucrata extract, dried tremella extract, bletilla striata extract, Leonurus extract, Bulbus Lilii extract or sponge gourd One or more of extract；

6. the postoperative biology cellulose facial mask of laser beautifying made from the preparation method as described in any one of Claims 1 to 5 is used, It is characterized in that：The postoperative biology cellulose facial mask of laser beautifying has double-decker, by upper strata tight section and lower floor's porosity and looseness Part is formed, and the aqueous solution, emulsion or the cream of the extract containing natural component are filled with the hole of double-decker；

The upper strata tight section is the Nanofiber Network being interweaved overlapped to form by cellulose nano-fibrous stratiform, on The density of layer tight section is 30~50kg/m³；

Lower floor's porosity and looseness part is by the cellulose nano-fibrous interconnected loose structure formed, while its hole wall There is cellulose nano-fibrous network, the density of lower floor's porosity and looseness part is 10~20kg/m on surface³；

The upper strata tight section passes through the intramolecular in β -1,4- grape sugar chains and intermolecular hydrogen with lower floor's porosity and looseness part Key is combined closely.

7. the postoperative biology cellulose facial mask of laser beautifying according to claim 6, it is characterised in that in the double-decker The content of the aqueous solution of the extract containing natural component, emulsion or cream is 0.1~3wt%；

The thickness of the postoperative biology cellulose facial mask of laser beautifying is 0.5~3mm, and the thickness of its tight section at the middle and upper levels is 0.01~0.1mm；

The saturation specific retention of the postoperative biology cellulose facial mask of laser beautifying is 180~360%, moisture-vapor transmission 5500 ~8500gm^-2·24h^-1。

8. the postoperative biology cellulose facial mask of laser beautifying according to claim 6, it is characterised in that the cellulose nanometer The average diameter of fiber is 20~60nm.

9. the postoperative biology cellulose facial mask of laser beautifying according to claim 6, it is characterised in that the upper strata compact part The average pore size divided is 3~5 μm, and the average pore size of lower floor's porosity and looseness part is 20~243 μm.