CN107582513B - Biological cellulose facial mask after laser cosmetology and preparation method thereof - Google Patents

Abstract

The invention relates to a biological cellulose facial mask after laser cosmetology and a preparation method thereof, wherein the preparation method comprises the following steps: adding the bacterial liquid into a culture emulsion, uniformly dispersing, standing and culturing to obtain a biological cellulose membrane, performing post-treatment, and compounding with a natural component extract to obtain the biological cellulose facial mask after laser cosmetology, wherein the bacterial liquid is a system in which bacterial strains are dispersed in water, and the culture emulsion is an oil-in-water emulsion, wherein the water phase is a strain culture solution, and the bacterial strains refer to microorganisms capable of biologically synthesizing cellulose nanofibers. The preparation method of the biological cellulose facial mask after the laser cosmetology is simple and easy to implement, green and pollution-free, the self structure of the material is easy to regulate and control in the preparation process, the finally prepared biological cellulose facial mask after the laser cosmetology has high water holding capacity and good facial spreadability, is simple and convenient to use and carry, can quickly and effectively relieve the uncomfortable symptoms of red, swollen, scorched, dry or water-deficient face and the like after the laser cosmetology, and has extremely good popularization value.

Description

Biological cellulose facial mask after laser cosmetology and preparation method thereof

Technical Field

The invention belongs to the field of medical materials, and relates to a biological cellulose facial mask and a preparation method thereof, in particular to a biological cellulose facial mask which can be applied to facial skin and used after laser cosmetology and a preparation method thereof.

Background

In recent years, with the development of laser medical theory and technology, laser beauty has become a fast, efficient and safe beauty technology. The laser beauty treatment has incomparable superiority with the traditional treatment, and has the advantages of less bleeding, less pain, high operation precision, short time, less scars and the like. With the popularization of laser beauty, the laser beauty consumables related to the laser beauty consumables have a wide market.

The laser is monochromatic light with certain penetrating power, is accurate in focusing, is usually applied to the cosmetic surgery process, mainly acts on human tissues and generates high heat on local parts of the human tissues, and therefore the cosmetic or therapeutic purpose is achieved. In the laser beauty treatment process, laser and skin tissues can generate strong heat effect, so that the epidermis, dermis or skin accessory organs of the skin can be burnt in different degrees, the phenomena of tissue drying and water shortage are inevitably caused, and the physiological functions of the skin such as protection, moisture preservation, anti-inflammation, sun protection and the like are influenced. For the commonly used non-exfoliative laser beauty treatment, the clinical manifestations are that slight red swelling and burning sensation appear at the treatment part after the operation, and even obvious phenomena such as edema, blister and blood seepage appear in severe cases. Therefore, the treatment part needs to be quickly and effectively cooled and moisturized and the wound repair is accelerated at the same time in the laser cosmetology postoperative care.

Patent CN104474577B discloses a dressing liquid for laser postoperative beauty, which is applied on the whole face after laser beauty operation, and has the functions of moisturizing and accelerating wound repair. However, the liquid dressing liquid is inconvenient to apply and has a very limited cooling effect. Patent CN201310141257.4 discloses a laser treatment postoperative repair mask prepared by adopting bacterial cellulose, which has a structure that the upper surface is dense and gradually transits downwards to the lower surface is loose. The structure lacks micron-sized macropores, so that the drug loading and water holding capacity are small in actual use, and the requirement on use time is difficult to meet.

Therefore, the research on the post-laser cosmetology biological cellulose facial mask which has the advantages of high water content, high drug loading rate, convenient use, simple and convenient preparation and easy regulation and control of the material structure in the preparation process and the preparation method thereof have extremely important significance.

Disclosure of Invention

The invention aims to solve the problems of inconvenient use, low drug-loading rate, low water-holding capacity, complex preparation process and difficult regulation and control of the structure of the material in the preparation process of a biological cellulose facial mask after laser cosmetology in the prior art, and provides the biological cellulose facial mask after laser cosmetology, which has the advantages of high water content, high drug-loading rate, convenient use, simple and convenient preparation and easy regulation and control of the structure of the material in the preparation process, and the preparation method thereof.

In order to achieve the purpose, the invention adopts the technical scheme that:

a preparation method of a biological cellulose facial mask after laser cosmetology comprises adding a bacterial solution into a culture emulsion, uniformly dispersing, standing and culturing to obtain a biological cellulose membrane, and post-treating and then compounding with a natural component extract to obtain the biological cellulose facial mask after laser cosmetology; the bacterial liquid is a system in which bacterial strains are dispersed in water, the culture emulsion is an oil-in-water emulsion, the water phase is a bacterial strain culture liquid, and the bacterial strains refer to microorganisms capable of biologically synthesizing cellulose nanofibers.

As a preferred technical scheme:

the preparation method of the biological cellulose facial mask after the laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the strain culture solution comprises the following components in percentage by weight: 2-5 wt% of glucose, 0.05-0.5 wt% of peptone, 0.05-0.5 wt% of yeast extract, 0.01-0.1 wt% of citric acid, 0.02-0.2 wt% of disodium hydrogen phosphate, 0.01-0.1 wt% of potassium dihydrogen phosphate and the balance of water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying different components to obtain a culture emulsion with a pH value of 5-7, wherein the culture emulsion comprises the following components in parts by weight: 50-80 parts of a strain culture solution, 5-30 parts of oil, 3-15 parts of an emulsifier and 5-22 parts of a thickening stabilizer;

unfreezing the refrigerated strain at room temperature, placing the refrigerated strain into a strain culture solution, carrying out shake culture at 28-32 ℃ for 6-24 h, supplementing and adding the strain culture solution, uniformly mixing, and carrying out standing culture at 28-32 ℃ for 3-5 days to obtain a bacterial solution, wherein the rotating speed of a shaker during shake culture is 50-150 r/min, and the supplemented and added strain culture solution is 10-100 times of the volume of the original strain culture solution;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 2-5 days at the temperature of 28-32 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 10-1000;

(4) post-treatment;

cutting a biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 1-4 wt%, boiling and keeping for 0.5-2 hours, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

dehydrating the biological cellulose facial mask base material until the water content is 40-80 wt%, then soaking the biological cellulose facial mask base material in an aqueous solution, emulsion or cream containing 10-30 wt% of natural component extracts, standing for 10-30 min, packaging and sterilizing to obtain the biological cellulose facial mask after laser cosmetology.

The preparation method of the biological cellulose facial mask after laser cosmetology comprises the following steps of (2), wherein the oil is more than one of peanut oil, coconut oil, soybean oil or mineral oil;

the emulsifier is more than one of polysorbate, sucrose fatty acid ester, hydrogenated lecithin or polyoxyethylene hydrogenated castor oil;

the thickening stabilizer is more than one of gelatin, sodium alginate, chitosan, sodium hyaluronate or sodium carboxymethylcellulose.

In the method for preparing the bio-cellulose facial mask after laser cosmetology, in the step (2), the bacterial strain is more than one of acetobacter gluconicum, acetobacter, acetobacter pasteurianus, gluconobacter, agrobacterium tumefaciens, rhizobium, sarcina, pseudomonas cepacia, pseudomonas cocoi or campylobacter jejuni; the species of the strain of the present invention include, but are not limited to, microorganisms capable of biosynthesis of cellulose nanofibers can be used as the strain of the present invention.

The number of the strain cells in unit volume in the bacterial liquid is 1.0 multiplied by 10³～1.0×10⁶one/mL.

According to the preparation method of the biological cellulose facial mask after the laser cosmetology, in the step (5), the dehydration is performed by centrifugal draining, vacuum drying or mechanical squeezing;

the natural component extract is more than one of radix Scutellariae extract, radix moutan extract, flos Matricariae Chamomillae extract, herba et Gemma Agrimoniae extract, fructus Chaenomelis extract, herba Saussureae Involueratae extract, Tremella extract, rhizoma Bletillae extract, herba Leonuri extract, Bulbus Lilii extract or fructus Luffae extract;

the sterilization refers to high-energy ray irradiation sterilization, pressure steam sterilization or electron beam sterilization.

The invention also provides the biological cellulose facial mask after the laser cosmetology, which is prepared by the preparation method and has a double-layer structure, wherein the biological cellulose facial mask after the laser cosmetology is composed of an upper compact part and a lower porous loose part, and the pores of the double-layer structure are filled with aqueous solution, emulsion or cream containing natural component extracts;

the upper-layer compact part is a mutually-interwoven nanofiber network formed by overlapping cellulose nanofibers in a layered manner, and the density of the upper-layer compact part is 30-50 kg/m³；

The lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of a pore wall of the lower porous loose part, and the density of the lower porous loose part is 10-20 kg/m³(ii) a The mutually communicated porous networks can ensure that biological signals, oxygen and nutrient substances enter the material, improve the proliferation and migration speed of cells and promote the vascularization of tissues and the formation of new tissues; the nanofiber material with high porosity and specific surface area can affect the interaction of cells and materials, promote substance transmission, and improve the cell behaviors such as cell adhesion, proliferation, differentiation, matrix expression and the like. The lower porous loose part of the biological cellulose facial mask is a porous loose structure which is formed by the biological cellulose nano fibers which are mutually staggered and has micro-pores and nano fibers, has the common advantages of the porous network and the nano fiber material, can promote cell adhesion and proliferation, guides cells to enter the material, and acceleratesVascularization of the surrounding tissue, thereby significantly accelerating the repair of skin wounds.

The upper dense part and the lower porous loose part are tightly combined through intramolecular and intermolecular hydrogen bonds in beta-1, 4-glucose chains.

As a preferred technical scheme:

the content of the water solution, the lotion or the cream containing the natural component extract in the double-layer structure of the biological cellulose facial mask after the laser cosmetology is 0.1-3 wt%;

the thickness of the biological cellulose facial mask after the laser cosmetology is 0.5-3 mm, wherein the thickness of the compact part of the upper layer is 0.01-0.1 mm; the normal human skin thickness is 0.5-4 mm, and the difference of the skin thickness of different physiological parts is larger. Therefore, there is a need to control the thickness of the bio-cellulose mask. The thickness of the material can be accurately regulated and controlled by regulating the culture time, the number of strains, the using amount of culture emulsion and the like, and the proportional structure of the double-layer structure can be further regulated by changing the viscosity of a culture system. The thickness of the compact part of the upper layer has obvious influence on the cell barrier effect, the water vapor transmission rate, the mechanical property and the like of the biological cellulose mask; and the lower porous loose part structure can influence the indexes of the biological cellulose mask, such as water absorption performance, cell proliferation rate, vascularization and the like. Therefore, the thickness and the ratio of the upper layer to the lower layer of the bio-cellulose mask should be comprehensively considered according to the actual application when designing the material.

The saturated water holding rate of the biological cellulose facial mask after laser cosmetology is 180-360%, and the water vapor transmission rate is 5500-8500 g.m^-2·24h^-1。

The cellulose nano-fiber of the biological cellulose facial mask after laser cosmetology has the average diameter of 20-60 nm.

The post-laser-cosmetology biological cellulose facial mask comprises an upper dense part and a lower porous loose part, wherein the average pore diameter of the upper dense part is 3-5 mu m, and the average pore diameter of the lower porous loose part is 20-243 mu m.

The invention mechanism is as follows:

the invention aims to prepare a biological cellulose facial mask which has a double-layer structure of compact upper layer and loose and porous lower layer and is similar to a human skin tissue acellular matrix after laser cosmetology. The traditional culture method of the biological cellulose membrane comprises the steps of uniformly dispersing strains in a strain culture solution of a water phase, and performing static fermentation to obtain a compact three-dimensional network structure formed by interweaving biological cellulose nanofibers. In the process of biological cellulose synthesis, strain cells are used as a biological synthesis reactor, nutrient substances generate cellulose microfibrils under the action of enzyme catalysis, the strain cells carry microfibril bundles to randomly move in a water phase of culture emulsion, strains can only biologically synthesize cellulose fibers in the water phase due to the steric hindrance of oil phase liquid drops, so that a porous structure of a biological cellulose lower layer is formed, a thin-layer water phase liquid surface is formed on the water phase on the emulsion liquid surface due to the action of surface tension and the self gravity of the oil phase liquid drops on the upper surface of a culture system, and the culture of the strain is similar to the traditional mode in a small area, so that a dense biological cellulose film on the upper layer is formed on the upper surface of the culture system. By adopting the preparation method, strain cells are uniformly dispersed in the water phase of the culture system, so that the formed biological cellulose mask after laser cosmetology is of an integral structure, the upper dense part and the lower porous loose part are formed by continuous cellulose nano fibers which are interwoven with each other through intramolecular and intermolecular hydrogen bond combination, no obvious external force stratification phenomenon exists, meanwhile, the thickening agent in the culture emulsion can adjust the viscosity of the culture system, and the distribution of oil phase liquid drops and the dissolved oxygen index in the system are controlled, so that the structures of the upper dense part and the lower porous loose part in the biological cellulose aggregate are further adjusted.

Has the advantages that:

(1) the preparation method of the biological cellulose facial mask after the laser cosmetology is simple and easy to implement, green and pollution-free, and the finally prepared biological cellulose facial mask after the laser cosmetology has high water holding capacity, good facial spreadability and simple and convenient use and carrying.

(2) The biological cellulose facial mask after laser cosmetology has a double-layer structure, and can be better attached to the surface of the skin. The upper compact part can reduce water volatilization, the lower porous loose part has large drug-loading rate and high water-holding capacity, and the natural component extract can be more effectively transferred to the skin by contacting with the skin, so that the skin water can be continuously, quickly and effectively supplemented, and the wound repair is accelerated.

(3) The biological cellulose facial mask after the laser cosmetology can quickly and effectively relieve the uncomfortable symptoms of red, swollen, scorching, dryness or water shortage and the like of the face after the non-peeling laser cosmetology.

Drawings

FIG. 1 is a schematic cross-sectional view of a bio-cellulose mask of the present invention after laser cosmetology;

FIG. 2 is a scanning electron micrograph of a cross-section of a bio-cellulose mask after laser cosmetology in accordance with the present invention;

FIG. 3 is a scanning electron micrograph of a cross-section of the dense portion of the upper layer of FIG. 2;

FIG. 4 is a scanning electron micrograph of a cross-section of the porous, porous portion of the lower layer of FIG. 2;

wherein, 1 is an upper layer compact part, and 2 is a lower layer porous loose part.

Detailed Description

The invention will be further illustrated with reference to specific embodiments. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.

Example 1

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 2wt%, the content of peptone is 0.3 wt%, the content of yeast extract is 0.5wt%, the content of citric acid is 0.05 wt%, the content of disodium hydrogen phosphate is 0.02 wt%, the content of potassium dihydrogen phosphate is 0.1wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 50 parts by weight of strain culture solution, 6 parts by weight of peanut oil, 15 parts by weight of polysorbate and 5 parts by weight of gelatin to obtain a culture emulsion with a pH value of 5;

thawing refrigerated gluconacetobacter at room temperature, placing into strain culture solution, shake culturing at 28 deg.C for 7 hr, supplementing and adding strain culture solution, mixing well, and standing and culturing at 32 deg.C for 3 days to obtain bacterial liquid, wherein the rotation speed of shaking table is 100r/min, the supplemented strain culture solution is 20 times of original strain culture solution volume, and the number of strain cells in unit volume of the obtained bacterial liquid is 1.0 × 10³Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and standing and culturing for 3 days at 28 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 30;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 1wt%, boiling and keeping for 0.5 hour, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

centrifugally draining the biological cellulose facial mask base material until the water content is 40 wt%, then soaking in cream containing 10 wt% of radix Scutellariae extract and radix moutan extract (mass ratio is 1:1), standing for 30min, packaging, and sterilizing by high-energy ray irradiation to obtain the biological cellulose facial mask after laser cosmetology.

As shown in fig. 1 to 2, the post-laser-cosmetology biocellulose mask prepared by the above steps has a double-layer structure consisting of an upper dense part 1 and a lower porous loose part 2, and the pores of the double-layer structure are filled with 0.1wt% of cream containing scutellaria baicalensis root extract and moutan root extract, which are tightly bound by intramolecular and intermolecular hydrogen bonds in β -1, 4-glucose chains. As shown in FIG. 3, the upper dense part 1 is a network of mutually interwoven nanofibers formed by layering and overlapping cellulose nanofibers, and the measured density is 40kg/m³The average pore diameter is 3 mu m; as shown in FIG. 4, the lower porous loose part 2 is an interconnected porous structure composed of cellulose nanofibers, and a network of cellulose nanofibers is present on the surface of the pore walls, and the measured density is 20kg/m³And an average pore diameter of 150 μm, wherein the average diameter of the cellulose nanofibers is 46 nm.

The obtained biocellulose mask after laser cosmetology has a thickness of 1mm, wherein the upper dense part has a thickness of 0.02mm, the saturated water holding rate of the biocellulose mask after laser cosmetology is 200%, and the water vapor transmission rate is 5700 g.m^-2·24h^-1。

Example 2

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 3wt%, the content of peptone is 0.10 wt%, the content of yeast extract is 0.05 wt%, the content of citric acid is 0.09 wt%, the content of disodium hydrogen phosphate is 0.05 wt%, the content of potassium dihydrogen phosphate is 0.01 wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 52 parts by weight of strain culture solution, 5 parts by weight of coconut oil, 3 parts by weight of sucrose fatty acid ester and 22 parts by weight of sodium alginate to obtain culture emulsion with the pH value of 7;

thawing refrigerated acetobacter at room temperature, placing into strain culture solution, shake culturing at 28 deg.C for 24 hr, supplementing and adding strain culture solution, mixing well, and standing and culturing at 32 deg.C for 3 days to obtain bacterial liquid, wherein the rotation speed of shaking table is 60r/min, the supplemented strain culture solution is 30 times of original strain culture solution volume, and the number of strain cells in unit volume of the obtained bacterial liquid is 5.5 × 10³Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 2 days at 32 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 10;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 2wt%, boiling and keeping for 0.5 hour, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

vacuum drying the biological cellulose facial mask base material to water content of 45 wt%, soaking in cream containing 15 wt% of flos Matricariae Chamomillae extract and herba et Gemma Agrimoniae extract (mass ratio of 1:2), standing for 25min, packaging, and sterilizing with pressure steam to obtain the final product.

The bio-cellulose mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, the pores of the double-layer structure are filled with 0.3 wt% of cream containing chamomile extract and hairyvein agrimonia herb and bud extract, and the two are tightly combined through intramolecular and intermolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 30kg/m³The average pore diameter is 3 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 10.5kg/m³Average pore diameter of 20 mum, wherein the cellulose nanofibers have an average diameter of 20 nm.

The obtained biocellulose facial mask after laser cosmetology has a thickness of 1.5mm, wherein the upper dense part has a thickness of 0.08mm, the saturated water holding rate of the biocellulose facial mask after laser cosmetology is 180%, and the water vapor transmission rate is 5500 g.m^-2·24h^-1。

Example 3

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 4wt%, the content of peptone is 0.4 wt%, the content of yeast extract is 0.15 wt%, the content of citric acid is 0.1wt%, the content of disodium hydrogen phosphate is 0.02 wt%, the content of potassium dihydrogen phosphate is 0.08 wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 56 parts by weight of strain culture solution, 9 parts by weight of soybean oil, 5 parts by weight of hydrogenated lecithin and 21 parts by weight of chitosan to obtain culture emulsion with the pH value of 6;

thawing refrigerated bacillus aceticus at room temperature, placing the thawed bacillus aceticus in a strain culture solution, performing shake culture at 29 ℃ for 10 hours, supplementing and adding the strain culture solution, uniformly mixing, and performing standing culture at 29 ℃ for 3 days to obtain a bacterial solution, wherein the rotating speed of a shaker during shake culture is 50r/min, the supplemented and added strain culture solution is 10 times of the volume of the original strain culture solution, and the number of strain cells in unit volume of the obtained bacterial solution is 1.0 multiplied by 10⁴one/mL.

(3) Standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 3 days at the temperature of 28 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 70;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into 3wt% sodium hydroxide solution, boiling and keeping the solution for 1 hour, and cleaning the membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

mechanically squeezing the biological cellulose facial mask substrate until the water content is 50 wt%, then soaking in cream containing 20 wt% of pawpaw extract, snow lotus extract and tremella extract (mass ratio is 1:2:2), standing for 20min, packaging, and sterilizing by electron beam to obtain the biological cellulose facial mask after laser cosmetology.

The biological cellulose facial mask prepared by adopting the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, the pores of the double-layer structure are filled with 0.5wt% of cream containing papaya extract, snow lotus extract and tremella extract, and the two are tightly combined with intermolecular hydrogen bonds through intramolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 38kg/m³The average pore diameter is 4 μm; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 10kg/m³And an average pore diameter of 80 μm, wherein the average diameter of the cellulose nanofibers is 30 nm.

The obtained biocellulose mask after laser cosmetology had a thickness of 2.0mm, wherein the upper dense part had a thickness of 0.02mm, the saturated water holding rate of the biocellulose mask after laser cosmetology was 220%, and the water vapor transmission rate was 5800 g.m^-2·24h^-1。

Example 4

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 5wt%, the content of peptone is 0.05 wt%, the content of yeast extract is 0.1wt%, the content of citric acid is 0.08 wt%, the content of disodium hydrogen phosphate is 0.04 wt%, the content of potassium dihydrogen phosphate is 0.07 wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 65 parts by weight of strain culture solution, 12 parts by weight of mineral oil, 7 parts by weight of polyoxyethylene hydrogenated castor oil and 17 parts by weight of sodium hyaluronate to obtain culture emulsion with the pH value of 7;

thawing refrigerated Acetobacter pasteurianus at room temperature, placing in a strain culture solution, performing shake culture at 30 ℃ for 6h, supplementing and adding the strain culture solution, uniformly mixing, performing static culture at 28 ℃ for 4 days to obtain a bacterial solution, wherein the rotation speed of a shaker during shake culture is 90r/min, the supplemented and added strain culture solution is 40 times of the volume of the original strain culture solution, and the number of strain cells in unit volume of the obtained bacterial solution is 6.2 × 10⁵Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 2 days at the temperature of 30 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 90;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 4wt%, boiling and keeping for 2 hours, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose membrane substrate;

(5) compounding;

centrifugally draining the biological cellulose facial mask base material until the water content is 55 wt%, then soaking in cream containing 25 wt% of bletilla striata extract, motherwort extract and lily extract (mass ratio is 1:1:1), standing for 15min, packaging and sterilizing by adopting electron beams to obtain the biological cellulose facial mask after laser cosmetology.

The bio-cellulose facial mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein the pores of the double-layer structure are filled with 0.8 wt% of cream containing bletilla striata extract, leonurus extract and lily extract, and the two are tightly combined through intramolecular and intermolecular hydrogen bonds in beta-1, 4-glucose chains. The upper layer isThe dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 40kg/m³The average pore diameter is 4.2 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 11kg/m³And an average pore diameter of 20 μm, wherein the average diameter of the cellulose nanofibers is 28 nm.

The obtained biocellulose facial mask after laser cosmetology has a thickness of 0.5mm, wherein the upper dense part has a thickness of 0.06mm, the saturated water holding rate of the biocellulose facial mask after laser cosmetology is 270%, and the water vapor transmission rate is 8500 g.m^-2·24h^-1。

Example 5

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 2.5 wt%, the content of peptone is 0.25 wt%, the content of yeast extract is 0.2wt%, the content of citric acid is 0.07 wt%, the content of disodium hydrogen phosphate is 0.08 wt%, the content of potassium dihydrogen phosphate is 0.06 wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 68 parts by weight of strain culture solution, 17 parts by weight of a mixture of peanut oil and soybean oil (the mass ratio is 1:1), 8 parts by weight of a mixture of polysorbate and sucrose fatty acid ester (the mass ratio is 2:1) and 22 parts by weight of sodium carboxymethylcellulose to obtain a culture emulsion with a pH value of 6;

thawing refrigerated gluconobacter at room temperature, placing in a strain culture solution, performing shake culture at 29 deg.C for 8h, supplementing and adding the strain culture solution, mixing well, and performing static culture at 30 deg.C for 4 days to obtain a bacterial solution, wherein the rotation speed of the shaker during shake culture is 90r/min, the supplemented and added strain culture solution is 20 times of the original strain culture solution, and the number of strain cells in unit volume in the obtained bacterial solutionIs 1.0X 10⁶Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 5 days at 31 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 700;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 2wt%, boiling and keeping for 0.5 hour, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

vacuum drying the biological cellulose facial mask base material to water content of 60 wt%, soaking in 30wt% of emulsion containing rhizoma Bletillae extract, herba Leonuri extract, Bulbus Lilii extract and fructus Luffae extract (mass ratio of 1:1:2:2), standing for 10min, packaging, and sterilizing with electron beam to obtain the final product.

The bio-cellulose facial mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein 1wt% of emulsion containing bletilla striata extract, leonurus extract, lily extract and towel gourd extract is filled in pores of the double-layer structure, and the two are tightly combined through intramolecular and intermolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 31kg/m³The average pore diameter is 3.5 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 13.5kg/m³And an average pore diameter of 185 μm, wherein the average diameter of the cellulose nanofibers is 60 nm.

The obtained biocellulose mask after laser cosmetology had a thickness of 1.7mm, wherein the upper dense part had a thickness of 0.1mm, the saturated water holding rate of the biocellulose mask after laser cosmetology was 310%, and the water vapor transmission rate was 5500 g.m^-2·24h^-1。

Example 6

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 3.5 wt%, the content of peptone is 0.45 wt%, the content of yeast extract is 0.25 wt%, the content of citric acid is 0.06 wt%, the content of disodium hydrogen phosphate is 0.09 wt%, the content of potassium dihydrogen phosphate is 0.04 wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 50 parts by weight of strain culture solution, 20 parts by weight of a mixture of coconut oil and mineral oil (the mass ratio is 2:1), 12 parts by weight of a mixture of hydrogenated lecithin and polyoxyethylene hydrogenated castor oil (the mass ratio is 1:1) and 13 parts by weight of a mixture of gelatin and sodium alginate (the mass ratio is 2:1) to obtain a culture emulsion with the pH value of 6;

thawing refrigerated agrobacterium at room temperature, placing in a strain culture solution, performing shake culture at 31 ℃ for 11h, supplementing and adding the strain culture solution, uniformly mixing, performing static culture at 30 ℃ for 3 days to obtain a bacterial solution, wherein the rotation speed of a shaking table during shake culture is 110r/min, the supplemented and added strain culture solution is 60 times of the volume of the original strain culture solution, and the number of strain cells in unit volume of the obtained bacterial solution is 1.6 multiplied by 10⁴Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 2 days at 29 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 100;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 1wt%, boiling and keeping for 1.5 hours, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

mechanically squeezing the biological cellulose facial mask base material to water content of 65 wt%, soaking in 28 wt% emulsion containing Scutellariae radix extract, peony root extract, flos Matricariae Chamomillae extract and herba et Gemma Agrimoniae extract (mass ratio of 1:1:1:2), standing for 12min, packaging, and sterilizing with high-energy ray irradiation to obtain the final product.

The bio-cellulose mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein 1.2 wt% of emulsion containing scutellaria baicalensis root extract, peony root extract, matricaria flower extract and hairyvein agrimonia herb and bud extract is filled in pores of the double-layer structure, and the two are tightly combined through intramolecular and intermolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 50kg/m³The average pore diameter is 3.6 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 10kg/m³And an average pore diameter of 125 μm, wherein the average diameter of the cellulose nanofibers is 49 nm.

The obtained biological cellulose facial mask after laser cosmetology has a thickness of 1.6mm, wherein the upper dense part has a thickness of 0.1mm, the saturated water holding rate of the biological cellulose facial mask after laser cosmetology is 270%, and the water vapor transmission rate is 8000 g.m^-2·24h^-1。

Example 7

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 4.5 wt%, the content of peptone is 0.35 wt%, the content of yeast extract is 0.35 wt%, the content of citric acid is 0.05 wt%, the content of disodium hydrogen phosphate is 0.11 wt%, the content of potassium dihydrogen phosphate is 0.05 wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 60 parts by weight of strain culture solution, 23 parts by weight of peanut oil, a mixture of coconut oil and mineral oil (the mass ratio is 1:2:1), 15 parts by weight of a mixture of polysorbate and polyoxyethylene hydrogenated castor oil (the mass ratio is 1:1) and 12 parts by weight of a mixture of chitosan and sodium hyaluronate (the mass ratio is 1:1) to obtain a culture emulsion with the pH value of 7;

thawing refrigerated rhizobia at room temperature, placing in a strain culture solution, shake culturing at 32 deg.C for 13h, adding the strain culture solution, mixing, and standing at 32 deg.C for 5 days to obtain a bacterial solution, wherein the rotation speed of the shaker is 120r/min, the strain culture solution is 80 times of the original strain culture solution, and the number of strain cells in unit volume of the obtained bacterial solution is 8.0 × 10⁵Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 5 days at 31 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 200;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into 3wt% sodium hydroxide solution, boiling and keeping the solution for 1.2 hours, and cleaning the membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

centrifugally draining the biological cellulose facial mask base material until the water content is 70 wt%, then soaking in an aqueous solution containing 10 wt% of scutellaria baicalensis root extract, standing for 10min, packaging and sterilizing by adopting high-energy ray irradiation to obtain the biological cellulose facial mask after laser cosmetology.

The post-laser-cosmetology biocellulose mask prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, pores of the double-layer structure are filled with 1.4 wt% of aqueous solution containing scutellaria baicalensis root extract, and the two are tightly combined through intramolecular and intermolecular hydrogen bonds in beta-1, 4-glucose chains. The upper compact part is formed by overlapping cellulose nano-fibers in a layer mannerOf a network of mutually interwoven nanofibers having a measured density of 49kg/m³The average pore diameter is 3 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 18kg/m³And an average pore diameter of 20 μm, wherein the average diameter of the cellulose nanofibers is 40 nm.

The obtained biocellulose mask after laser cosmetology has a thickness of 3mm, wherein the upper dense part has a thickness of 0.05mm, the saturated water holding rate of the biocellulose mask after laser cosmetology is 220%, and the water vapor transmission rate is 5900 g.m^-2·24h^-1。

Example 8

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 5wt%, the content of peptone is 0.15 wt%, the content of yeast extract is 0.3 wt%, the content of citric acid is 0.03 wt%, the content of disodium hydrogen phosphate is 0.12 wt%, the content of potassium dihydrogen phosphate is 0.1wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 74 parts by weight of strain culture solution, 26 parts by weight of coconut oil, a mixture of soybean oil and mineral oil (the mass ratio is 2:1: 1), 4 parts by weight of polysorbate, a mixture of sucrose fatty acid ester and hydrogenated lecithin (the mass ratio is 2:1:2) and 10 parts by weight of a mixture of sodium alginate, chitosan and sodium hyaluronate (the mass ratio is 1:1:2) to obtain a culture emulsion with the pH value of 5;

thawing refrigerated sarcina at room temperature, placing in a strain culture solution, performing shake culture at 32 ℃ for 6h, supplementing and adding the strain culture solution, uniformly mixing, performing static culture at 28 ℃ for 3 days to obtain a bacterial solution, wherein the rotation speed of a shaking table during shake culture is 50r/min, the supplemented and added strain culture solution is 10 times of the volume of the original strain culture solution, and the volume of the obtained bacterial solution in the bacterial solution is singleThe number of cells of the inner strain was 3.0X 10⁴Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and standing and culturing for 3 days at 32 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 300;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 4wt%, boiling and keeping for 1.7 hours, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

vacuum drying the biological cellulose facial mask base material to water content of 75 wt%, soaking in 17 wt% emulsion of peony root extract, standing for 14min, packaging, and sterilizing with pressure steam to obtain the final product.

The bio-cellulose mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein the pores of the double-layer structure are filled with 1.8 wt% of emulsion containing the peony root extract, and the emulsion are tightly combined through intramolecular and intermolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 31kg/m³The average pore diameter is 3.3 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 18kg/m³And an average pore diameter of 65 μm, wherein the average diameter of the cellulose nanofibers is 35 nm.

The obtained biocellulose facial mask after laser cosmetology has a thickness of 0.9mm, wherein the upper dense part has a thickness of 0.01mm, the saturated water holding rate of the biocellulose facial mask after laser cosmetology is 200%, and the water vapor transmission rate is 7000 g.m^-2·24h^-1。

Example 9

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 2wt%, the content of peptone is 0.2wt%, the content of yeast extract is 0.4 wt%, the content of citric acid is 0.02 wt%, the content of disodium hydrogen phosphate is 0.14 wt%, the content of potassium dihydrogen phosphate is 0.03 wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 72 parts by weight of strain culture solution, 30 parts by weight of a mixture of peanut oil, coconut oil, soybean oil and mineral oil (the mass ratio is 1:2:2:1), 8 parts by weight of a mixture of sucrose fatty acid ester, hydrogenated lecithin and polyoxyethylene hydrogenated castor oil (the mass ratio is 1:1:2) and 8 parts by weight of a mixture of gelatin, sodium alginate, sodium hyaluronate and sodium carboxymethylcellulose (the mass ratio is 1:2:2:1) to obtain a culture emulsion with the pH value of 6;

thawing refrigerated Pseudomonas cepacia at room temperature, placing in strain culture solution, shake culturing at 29 deg.C for 15 hr, adding strain culture solution, mixing, and standing at 31 deg.C for 4 days to obtain bacterial liquid, wherein the rotation speed of shaking table is 130r/min, the strain culture solution is 85 times of original strain culture solution, and the number of strain cells in unit volume of the obtained bacterial liquid is 1.0 × 10³Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and standing and culturing for 4 days at 28 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 600;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into 3wt% sodium hydroxide solution, boiling and keeping for 0.6 hour, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

mechanically squeezing the biological cellulose facial mask substrate until the water content is 80wt%, soaking in 13 wt% of mother chrysanthemum extract water solution, standing for 18min, packaging, and sterilizing by high-energy ray irradiation to obtain the final product.

The bio-cellulose facial mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein the pores of the double-layer structure are filled with 2.1 wt% of aqueous solution containing chamomile extract, and the two are tightly combined with intermolecular hydrogen bonds through intramolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 37kg/m³The average pore diameter is 4 μm; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 12kg/m³And an average pore diameter of 200 μm, wherein the average diameter of the cellulose nanofibers is 20 nm.

The obtained biocellulose mask after laser cosmetology had a thickness of 1.5mm, wherein the upper dense part had a thickness of 0.05mm, the saturated water holding rate of the biocellulose mask after laser cosmetology was 190%, and the water vapor transmission rate was 8500 g.m^-2·24h^-1。

Example 10

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 3wt%, the content of peptone is 0.10 wt%, the content of yeast extract is 0.45 wt%, the content of citric acid is 0.01 wt%, the content of disodium hydrogen phosphate is 0.15 wt%, the content of potassium dihydrogen phosphate is 0.02 wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 80 parts by weight of strain culture solution, 5 parts by weight of a mixture of peanut oil and mineral oil (the mass ratio is 1:1), 3 parts by weight of a mixture of polysorbate, hydrogenated lecithin and polyoxyethylene hydrogenated castor oil (the mass ratio is 1:2:2) and 6 parts by weight of a mixture of sodium alginate, chitosan, sodium hyaluronate and sodium carboxymethylcellulose (the mass ratio is 1:1:2:1) to obtain a culture emulsion with the pH value of 7;

thawing refrigerated Pseudomonas cocoanut at room temperature, placing in strain culture solution, shake culturing at 30 deg.C for 22 hr, adding strain culture solution, mixing, and standing at 28 deg.C for 5 days to obtain bacterial liquid, wherein the rotation speed of shaking table is 140r/min, the strain culture solution is 90 times of original strain culture solution, and the number of strain cells in unit volume of the obtained bacterial liquid is 4.8 × 10³Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 5 days at 29 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 800;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 1wt%, boiling and keeping for 0.8 hour, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

centrifugally draining the biological cellulose facial mask substrate until the water content is 80wt%, soaking in 10 wt% of herba et Gemma Agrimoniae extract emulsion, standing for 21min, packaging, and sterilizing with electron beam to obtain the final product.

The bio-cellulose facial mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein the pores of the double-layer structure are filled with 2wt% of emulsion containing hairyvein agrimonia herb and bud extract, and the two parts are tightly combined with intermolecular hydrogen bonds through intramolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a mutually interwoven nanofiber network formed by overlapping cellulose nanofibers in a layered manner, and the measured density is 42kg/m³The average pore diameter is 4.9 μm; lower layer is provided withThe loose part of the pores is a mutually communicated porous structure consisting of cellulose nanofibers, and a cellulose nanofiber network exists on the surface of the pore wall of the loose part of the pores, and the measured density is 17.3kg/m³And an average pore diameter of 190 μm, wherein the average diameter of the cellulose nanofibers is 38 nm.

The obtained biological cellulose facial mask after laser cosmetology has a thickness of 0.5mm, wherein the upper dense part has a thickness of 0.1mm, the saturated water holding rate of the biological cellulose facial mask after laser cosmetology is 360%, and the water vapor transmission rate is 6400 g.m^-2·24h^-1。

Example 11

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 3.8 wt%, the content of peptone is 0.05 wt%, the content of yeast extract is 0.25 wt%, the content of citric acid is 0.01 wt%, the content of disodium hydrogen phosphate is 0.2wt%, the content of potassium dihydrogen phosphate is 0.01 wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 56 parts by weight of strain culture solution, 15 parts by weight of peanut oil, a mixture of coconut oil and mineral oil (the mass ratio is 1:1:2), 10 parts by weight of polysorbate, sucrose fatty acid ester, a mixture of hydrogenated lecithin and polyoxyethylene hydrogenated castor oil (the mass ratio is 1:1:2:1) and 5 parts by weight of a mixture of gelatin, sodium alginate, chitosan, sodium hyaluronate and sodium carboxymethylcellulose (the mass ratio is 1:1:2:2:1) to obtain a culture emulsion with the pH value of 5;

thawing refrigerated campylobacter jejuni at room temperature, placing in strain culture solution, shake culturing at 29 deg.C for 24 hr, supplementing and adding strain culture solution, mixing well, and standing and culturing at 31 deg.C for 4 days to obtain bacterial liquid, wherein the rotation speed of shaking table is 150r/min, the supplemented strain culture solution is 100 times of original strain culture solution volume, and the number of strain cells in unit volume in the obtained bacterial liquidIs 1.0X 10⁴Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 4 days at the temperature of 28 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 1000;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 4wt%, boiling and keeping for 0.9 hour, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

vacuum drying the biological cellulose facial mask base material to water content of 77 wt%, soaking in aqueous solution containing fructus Chaenomelis extract 11 wt%, standing for 15min, packaging, and sterilizing with high-energy ray irradiation to obtain the final product.

The biological cellulose facial mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein the pores of the double-layer structure are filled with aqueous solution containing papaya extract with the content of 3wt%, and the aqueous solution are tightly combined through intramolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 50kg/m³The average pore diameter is 3 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 10kg/m³And an average pore diameter of 243 μm, wherein the average diameter of the cellulose nanofibers is 35 nm.

The obtained biocellulose mask after laser cosmetology had a thickness of 2.2mm, wherein the upper dense part had a thickness of 0.03mm, the saturated water holding rate of the biocellulose mask after laser cosmetology was 280%, and the water vapor transmission rate was 8500 g.m^-2·24h^-1。

Example 12

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 2wt%, the content of peptone is 0.5wt%, the content of yeast extract is 0.5wt%, the content of citric acid is 0.05 wt%, the content of disodium hydrogen phosphate is 0.02 wt%, the content of potassium dihydrogen phosphate is 0.1wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 50 parts by weight of strain culture solution, 6 parts by weight of a mixture of coconut oil and mineral oil (the mass ratio is 1:1), 15 parts by weight of polysorbate and 5 parts by weight of a mixture of chitosan and sodium hyaluronate (the mass ratio is 1:2) to obtain a culture emulsion with the pH value of 6;

thawing refrigerated gluconacetobacter and onion pseudomonas (mass ratio of 1:2) at room temperature, placing into strain culture solution, shake-culturing at 28 deg.C for 24h, supplementing and adding strain culture solution, mixing well, and standing at 32 deg.C for 3 days to obtain bacterial solution, wherein the rotation speed of shaker during shake-culturing is 100r/min, the supplemented strain culture solution is 30 times of original strain culture solution volume, and the number of strain cells in unit volume of the obtained bacterial solution is 1.0 × 10³Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and standing and culturing for 3 days at 28 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 80;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 2wt%, boiling and keeping for 2 hours, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose membrane substrate;

(5) compounding;

mechanically squeezing the biological cellulose facial mask substrate until the water content is 64 wt%, soaking in 29 wt% aqueous solution of herba Saussureae Involueratae extract, standing for 10min, packaging, and sterilizing with electron beam to obtain the final product.

The biological cellulose facial mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein the pores of the double-layer structure are filled with 2.2 wt% of aqueous solution containing saussurea involucrate extract, and the two are tightly combined with intermolecular hydrogen bonds through the intramolecular of beta-1, 4-glucose chains. The upper dense layer is a network of interwoven nanofibers formed by the layered overlapping of cellulose nanofibers and having a measured density of 45kg/m³The average pore diameter is 4.5 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 19.6kg/m³And an average pore diameter of 28 μm, wherein the average diameter of the cellulose nanofibers is 28 nm.

The obtained biocellulose mask after laser cosmetology has a thickness of 3mm, wherein the upper dense part has a thickness of 0.06mm, the saturated water holding rate of the biocellulose mask after laser cosmetology is 210%, and the water vapor transmission rate is 6850 g.m^-2·24h^-1。

Example 13

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 2wt%, the content of peptone is 0.3 wt%, the content of yeast extract is 0.5wt%, the content of citric acid is 0.05 wt%, the content of disodium hydrogen phosphate is 0.02 wt%, the content of potassium dihydrogen phosphate is 0.1wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 60 parts by weight of strain culture solution, 7 parts by weight of peanut oil, 15 parts by weight of a mixture (mass ratio is 2:2) of sucrose fatty acid ester and hydrogenated lecithin and 20 parts by weight of sodium alginate to obtain a culture emulsion with a pH value of 5;

acetifying the refrigerated stalkThawing bacteria and Acetobacter pasteurianus (mass ratio of 2:1) at room temperature, placing into strain culture solution, shake-culturing at 29 deg.C for 24h, supplementing and mixing strain culture solution, standing and culturing at 32 deg.C for 3 days to obtain bacterial liquid, wherein the rotation speed of shaker during shake-culturing is 100r/min, the supplemented strain culture solution is 20 times of original strain culture solution volume, and the number of strain cells in unit volume in the obtained bacterial liquid is 5.6 × 10³Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and standing and culturing for 3 days at 28 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 30;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 4wt%, boiling and keeping for 1.1 hours, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

and centrifugally draining the biological cellulose facial mask base material until the water content is 56 wt%, then soaking in an aqueous solution containing 14 wt% of tremella fuciformis extract, standing for 30min, packaging and sterilizing by adopting pressure steam to obtain the post-laser-cosmetology biological cellulose facial mask.

The post-laser-cosmetology biocellulose mask prepared by the above steps has a double-layer structure composed of an upper dense part and a lower porous loose part, pores of the double-layer structure are filled with an aqueous solution containing a Tremella extract with a content of 2.4 wt%, and the two are tightly bonded with intermolecular hydrogen bonds through intramolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 46kg/m³The average pore diameter is 3.3 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 20kg/m³And an average pore diameter of 37 μm, wherein the average diameter of the cellulose nanofibers is 60 nm.

The obtained biocellulose mask after laser cosmetology has a thickness of 2mm, wherein the upper dense part has a thickness of 0.01mm, the saturated water holding rate of the biocellulose mask after laser cosmetology is 180%, and the water vapor transmission rate is 6200 g.m^-2·24h^-1。

Example 14

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 2wt%, the content of peptone is 0.3 wt%, the content of yeast extract is 0.5wt%, the content of citric acid is 0.05 wt%, the content of disodium hydrogen phosphate is 0.02 wt%, the content of potassium dihydrogen phosphate is 0.1wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 75 parts by weight of strain culture solution, 10 parts by weight of soybean oil, 15 parts by weight of polyoxyethylene hydrogenated castor oil and 5 parts by weight of a mixture (mass ratio is 1:1:1) of sodium alginate, chitosan and sodium hyaluronate to obtain a culture emulsion with a pH value of 7;

thawing refrigerated Acetobacter pasteurianus, Gluconobacter and Agrobacterium (mass ratio of 1:1:2) at room temperature, placing into strain culture solution, shake culturing at 28 deg.C for 24h, supplementing and adding strain culture solution, mixing well, standing and culturing at 32 deg.C for 4 days to obtain bacterial solution, wherein the rotation speed of shake culturing at shake bed is 100r/min, the supplemented strain culture solution is 20 times of original strain culture solution volume, and the number of strain cells in unit volume of the obtained bacterial solution is 3.5 × 10⁵Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and standing and culturing for 3 days at 28 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 300;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into 3wt% sodium hydroxide solution, boiling and keeping the solution for 1.5 hours, and cleaning the membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

vacuum drying the biological cellulose facial mask base material to water content of 48 wt%, soaking in 15 wt% bletilla striata extract emulsion, standing for 30min, packaging, and sterilizing with pressure steam to obtain the final product.

The bio-cellulose facial mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein the pores of the double-layer structure are filled with 2.5 wt% of emulsion containing bletilla striata extract, and the emulsion are tightly combined through intramolecular and intermolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 38kg/m³The average pore diameter is 3 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 12.8kg/m³And an average pore diameter of 180 μm, wherein the average diameter of the cellulose nanofibers is 55 nm.

The obtained biocellulose mask after laser cosmetology has a thickness of 0.7mm, wherein the upper dense part has a thickness of 0.1mm, the saturated water holding rate of the biocellulose mask after laser cosmetology is 200%, and the water vapor transmission rate is 6500 g.m^-2·24h^-1。

Example 15

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 5wt%, the content of peptone is 0.3 wt%, the content of yeast extract is 0.5wt%, the content of citric acid is 0.05 wt%, the content of disodium hydrogen phosphate is 0.02 wt%, the content of potassium dihydrogen phosphate is 0.1wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 75 parts by weight of strain culture solution, 20 parts by weight of a mixture of coconut oil and soybean oil (the mass ratio is 2:2), 15 parts by weight of polysorbate and 22 parts by weight of gelatin to obtain a culture emulsion with a pH value of 5;

thawing refrigerated gluconacetobacter, pseudomonas cocoi and campylobacter jejuni (mass ratio of 1:1:1) at room temperature, placing into strain culture solution, shake culturing at 28 deg.C for 24h, supplementing and adding strain culture solution, mixing well, and standing and culturing at 32 deg.C for 3 days to obtain bacterial liquid, wherein the rotation speed of shaking table is 100r/min during shake culturing, the supplemented strain culture solution is 20 times of original strain culture solution volume, and the number of strain cells in unit volume in the obtained bacterial liquid is 1.0 × 10⁶Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and standing and culturing for 3 days at 28 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 300;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 1wt%, boiling and keeping for 0.5 hour, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

mechanically squeezing the biological cellulose facial mask substrate until the water content is 80wt%, soaking in 10 wt% of herba Leonuri extract water solution, standing for 10min, packaging, and sterilizing with electron beam to obtain the final product.

The bio-cellulose mask after laser cosmetology prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein the pores of the double-layer structure are filled with 0.8 wt% of an aqueous solution containing a motherwort extract, and the two are tightly combined with intermolecular hydrogen bonds through intramolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is made of cellulose nanoThe fibers were layered to form an interwoven nanofiber network having a measured density of 50kg/m³The average pore diameter is 3.1 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 17.7kg/m³And an average pore diameter of 80 μm, wherein the average diameter of the cellulose nanofibers is 46 nm.

The obtained biocellulose mask after laser cosmetology has a thickness of 2.3mm, wherein the upper dense part has a thickness of 0.05mm, the saturated water holding rate of the biocellulose mask after laser cosmetology is 180%, and the water vapor transmission rate is 7700 g.m^-2·24h^-1。

Example 16

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 2wt%, the content of peptone is 0.3 wt%, the content of yeast extract is 0.5wt%, the content of citric acid is 0.05 wt%, the content of disodium hydrogen phosphate is 0.02 wt%, the content of potassium dihydrogen phosphate is 0.1wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 55 parts by weight of strain culture solution, 6 parts by weight of mineral oil, 3 parts by weight of polysorbate, a mixture (in a mass ratio of 2:1:1:2) of sucrose fatty acid ester, hydrogenated lecithin and polyoxyethylene hydrogenated castor oil and 5 parts by weight of chitosan to obtain culture emulsion with a pH value of 6;

thawing refrigerated acetobacter acetogenins, acetification bacillus, acetobacter pasteurianus and gluconobacter (mass ratio is 1:1:2:1) at room temperature, placing the thawed acetogenins, acetification bacillus, acetogenins and gluconobacter in a strain culture solution, performing shake cultivation at 28 ℃ for 24 hours, supplementing and adding the strain culture solution, uniformly mixing, performing standing cultivation at 32 ℃ for 3 days to obtain a bacterial solution, wherein the rotation speed of a shaker during shake cultivation is 100r/min, the supplemented and added strain culture solution is 20 times of the volume of the original strain culture solution, and the obtained bacterial solution is added into the bacterial solutionThe number of cells of the strain per unit volume was 2.0X 10³Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 5 days at the temperature of 28 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 120;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 4wt%, boiling and keeping for 0.9 hour, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

centrifugally draining the biological cellulose facial mask base material until the water content is 80wt%, then soaking in an aqueous solution containing 10 wt% of lily extract, standing for 22min, packaging, and sterilizing by high-energy ray irradiation to obtain the biological cellulose facial mask after laser cosmetology.

The post-laser-cosmetology bio-cellulose facial mask prepared by the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, wherein the pores of the double-layer structure are filled with 3wt% of aqueous solution containing lily extract, and the two are tightly combined with intermolecular hydrogen bonds through intramolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 39kg/m³The average pore diameter is 5 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 14kg/m³And an average pore diameter of 143 μm, wherein the average diameter of the cellulose nanofibers is 20 nm.

The obtained biocellulose mask after laser cosmetology has a thickness of 3mm, wherein the upper dense part has a thickness of 0.01mm, the saturated water holding rate of the biocellulose mask after laser cosmetology is 300%, and the water vapor transmission rate is 8300 g.m^-2·24h^-1。

Example 17

A preparation method of a biological cellulose facial mask after laser cosmetology comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution, wherein the content of glucose in the strain culture solution is 3wt%, the content of peptone is 0.3 wt%, the content of yeast extract is 0.5wt%, the content of citric acid is 0.05 wt%, the content of disodium hydrogen phosphate is 0.02 wt%, the content of potassium dihydrogen phosphate is 0.1wt%, and the balance is water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying 58 parts by weight of strain culture solution, 6 parts by weight of peanut oil, 15 parts by weight of hydrogenated lecithin and 5 parts by weight of a mixture (mass ratio of 2:1) of sodium hyaluronate and sodium carboxymethylcellulose to obtain a culture emulsion with a pH value of 5;

thawing refrigerated agrobacterium, rhizobium, sarcina and pseudomonas cepacia (mass ratio of 2:1:2:1) at room temperature, placing the thawed agrobacterium, rhizobium, sarcina and pseudomonas cepacia (mass ratio of 2:1:2:1) in a strain culture solution, performing shake culture at 28 ℃ for 24 hours, supplementing and uniformly mixing the strain culture solution, performing standing culture at 32 ℃ for 3 days to obtain a bacterial solution, wherein the rotating speed of a shaker during shake culture is 100r/min, the supplemented and added strain culture solution is 20 times of the volume of an original strain culture solution, and the number of strain cells in unit volume of the obtained bacterial solution is 3.8 multiplied by 10⁵Per mL;

(3) standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and standing and culturing for 3 days at 28 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 500;

(4) post-treatment;

cutting the biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 2wt%, boiling and keeping for 2 hours, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose membrane substrate;

(5) compounding;

mechanically squeezing the biological cellulose facial mask substrate until the water content is 50 wt%, soaking in cream containing 20 wt% of fructus Luffae extract, standing for 19min, packaging, and sterilizing with pressure steam to obtain the final product.

The bio-cellulose facial mask after laser cosmetology prepared by adopting the steps has a double-layer structure consisting of an upper compact part and a lower porous loose part, the pores of the double-layer structure are filled with 0.5wt% of cream containing the towel gourd extract, and the cream are tightly combined through intramolecular and intermolecular hydrogen bonds in beta-1, 4-glucose chains. The upper dense part is a network of mutually interwoven nanofibers formed by the layered overlapping of cellulose nanofibers, and the measured density is 40kg/m³The average pore diameter is 3.5 mu m; the lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of the pore wall of the lower porous loose part, and the measured density is 11.8kg/m³And an average pore diameter of 200 μm, wherein the average diameter of the cellulose nanofibers is 56 nm.

The obtained biocellulose mask after laser cosmetology has a thickness of 0.5mm, wherein the upper dense part has a thickness of 0.02mm, the saturated water holding rate of the biocellulose mask after laser cosmetology is 280%, and the water vapor transmission rate is 7950 g.m^-2·24h^-1。

Claims (5)

1. A preparation method of a biological cellulose facial mask after laser cosmetology is characterized by comprising the following steps: the method comprises the following specific steps:

(1) preparing a strain culture solution;

uniformly mixing different components to obtain a strain culture solution;

the strain culture solution comprises the following components in percentage by weight: 2-5 wt% of glucose, 0.05-0.5 wt% of peptone, 0.05-0.5 wt% of yeast extract, 0.01-0.1 wt% of citric acid, 0.02-0.2 wt% of disodium hydrogen phosphate, 0.01-0.1 wt% of potassium dihydrogen phosphate and the balance of water;

(2) preparing culture emulsion and bacterial liquid;

homogenizing and emulsifying different components to obtain a culture emulsion with the pH value of 5-7;

the culture emulsion comprises the following components in parts by weight: 50-80 parts of a strain culture solution, 5-30 parts of oil, 3-15 parts of an emulsifier and 5-22 parts of a thickening stabilizer;

unfreezing the refrigerated strain at room temperature, placing the refrigerated strain into a strain culture solution, carrying out shake culture at 28-32 ℃ for 6-24 h, supplementing and adding the strain culture solution, uniformly mixing, and carrying out standing culture at 28-32 ℃ for 3-5 days to obtain a bacterial solution, wherein the rotating speed of a shaker during shake culture is 50-150 r/min, and the supplemented and added strain culture solution is 10-100 times of the volume of the original strain culture solution;

the strain is more than one of gluconacetobacter, acetobacter, aceticus, acetobacter pasteurianus, gluconobacter, agrobacterium tumefaciens, rhizobium, sarcina, pseudomonas cepacia, pseudomonas cocoi or campylobacter jejuni;

the number of the strain cells in unit volume in the bacterial liquid is 1.0 multiplied by 10³~1.0×10⁶Per mL

(3) Standing and culturing;

adding the bacterial liquid into the culture emulsion, uniformly dispersing, and then standing and culturing for 2-5 days at the temperature of 28-32 ℃ to obtain a biological cellulose membrane, wherein the volume ratio of the bacterial liquid to the culture emulsion is 1: 10-1000;

(4) post-treatment;

cutting a biological cellulose membrane into a shape of a facial mask, soaking the biological cellulose membrane into a sodium hydroxide solution with the concentration of 1-4 wt%, boiling and keeping for 0.5-2 hours, and cleaning the biological cellulose membrane with purified water until the pH value is 7.0 to obtain a biological cellulose facial mask substrate;

(5) compounding;

dehydrating the biological cellulose facial mask base material until the water content is 40-80 wt%, then soaking the biological cellulose facial mask base material in an aqueous solution, emulsion or cream containing 10-30 wt% of natural component extracts, standing for 10-30 min, packaging and sterilizing to obtain the biological cellulose facial mask after laser cosmetology;

the dehydration adopts a centrifugal draining method, a vacuum drying method or a mechanical squeezing method;

the natural component extract is more than one of radix Scutellariae extract, radix moutan extract, flos Matricariae Chamomillae extract, herba et Gemma Agrimoniae extract, fructus Chaenomelis extract, herba Saussureae Involueratae extract, Tremella extract, rhizoma Bletillae extract, herba Leonuri extract, Bulbus Lilii extract or fructus Luffae extract;

the sterilization refers to high-energy ray irradiation sterilization, pressure steam sterilization or electron beam sterilization;

the biological cellulose facial mask after laser cosmetology has a double-layer structure, which consists of an upper compact part and a lower porous loose part, wherein the pores of the double-layer structure are filled with aqueous solution, emulsion or cream containing natural component extracts;

the upper-layer compact part is a mutually-interwoven nanofiber network formed by overlapping cellulose nanofibers in a layered manner, and the density of the upper-layer compact part is 30-50 kg/m³；

The lower porous loose part is a mutually communicated porous structure consisting of cellulose nanofibers, a cellulose nanofiber network exists on the surface of a pore wall of the lower porous loose part, and the density of the lower porous loose part is 10-20 kg/m³；

The upper dense part and the lower porous loose part are tightly combined through intramolecular and intermolecular hydrogen bonds in beta-1, 4-glucose chains.

2. The method for preparing the bio-cellulose facial mask after laser cosmetology according to the claim 1, characterized in that, in the step (2), the oil is more than one of peanut oil, coconut oil, soybean oil or mineral oil;

the emulsifier is more than one of polysorbate, sucrose fatty acid ester, hydrogenated lecithin or polyoxyethylene hydrogenated castor oil;

the thickening stabilizer is more than one of gelatin, sodium alginate, chitosan, sodium hyaluronate or sodium carboxymethylcellulose.

3. The preparation method of the bio-cellulose facial mask after the laser cosmetology according to claim 1, characterized in that the content of the water solution, the lotion or the cream containing the natural component extract in the double-layer structure is 0.1-3 wt%;

the thickness of the biological cellulose facial mask after the laser cosmetology is 0.5-3 mm, wherein the thickness of the compact part of the upper layer is 0.01-0.1 mm;

the saturated water holding rate of the biological cellulose facial mask after laser cosmetology is 180-360%, and the water vapor transmission rate is 5500-8500 g.m^-2·24h^-1。

4. The method for preparing the bio-cellulose facial mask after the laser cosmetology according to claim 1, wherein the average diameter of the cellulose nano-fibers is 20-60 nm.

5. The method for preparing a bio-cellulose facial mask after laser cosmetology according to claim 1, wherein the average pore diameter of the dense part of the upper layer is 3-5 μm, and the average pore diameter of the porous and loose part of the lower layer is 20-243 μm.

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